CN109593010A - A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer - Google Patents

A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer Download PDF

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Publication number
CN109593010A
CN109593010A CN201910010560.8A CN201910010560A CN109593010A CN 109593010 A CN109593010 A CN 109593010A CN 201910010560 A CN201910010560 A CN 201910010560A CN 109593010 A CN109593010 A CN 109593010A
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foliar fertilizer
organic
compound
preparation
organic foliar
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陈家童
喻其林
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Tianjin New Platform Biological Technology Co Ltd
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Tianjin New Platform Biological Technology Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C11/00Other nitrogenous fertilisers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • C05G5/23Solutions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The invention discloses a kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer.The invention is can generate the lactobacillus of lactic acid and nisin, streptococcus lactis as fermentation strain, the fermentation liquid rich in lactic acid and nisin is obtained under the conditions of constant temperature incubation, and then by adding the plant nutrient ingredients such as potassium hydroxide, compound organic matter, microelement in fermentation liquid, high compound disease-resistant organic foliar fertilizer is prepared.This method have many advantages, such as it is low in cost, prepare it is simple, environmentally protective, production overall process without any waste water, waste, exhaust gas discharge, prepared organic foliar fertilizer has the characteristics that nutrition is comprehensive, enhancing plant disease-resistant ability, has no toxic side effect, bio-safety problem, crop quality and yield is not caused to significantly improve, it is the Biological organic foliar fertilizer with real meaning, good economic benefit and social benefit can be generated.

Description

A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer
Technical field
The invention belongs to bio-organic fertilizer fields, are related to novel organic foliar fertilizer exploitation, fruits and vegetables plantation, afforestation etc. Aspect, and in particular to the preparation and its application of compound organic foliar fertilizer.
Background technique
Foliar fertilizer is a kind of novel vegetable fertilizer.Compared with traditional rhizosphere fertilizer, foliar fertilizer has with strong points, suction Receive the advantages that fast, effect is good, environmental pollution is small, application is simple and convenient, can be good at making up rhizosphere fertilizer absorb it is slow, be lost it is tight The deficiencies of weight, becomes the hot spot that agricultural fertilizer is developed with applied in recent years.With carrying out in a deep going way for foliar fertilizer research, a series of leaves Sourdough product continues to bring out, such as compound amino acid foliar fertilizer, chelating type microelement foliage fertilizer, chelating type rare earth foliar fertilizer, corruption Grow sour foliar fertilizer etc..However, there are with low content of technology, content of organic matter difference is big, unbalanced nutrition (especially potassium for these products Content is low), do not have many defects such as disease-resistant performance, product quality be irregular, largely limit the big of foliar fertilizer Scale promotes and applies.Therefore, exploration is efficiently, green, environmental protection, nutrition is balanced, disease-resistant new foliar fertilizer is imperative.
In recent years, with the rapid development of biotechnology, the research increasing that organic fertilizer becomes new is prepared using fermentation process Long, this is because such method has, working condition is mild, reaction process is easily-controllable, nutritional ingredient is balanced, difficult utilize can be achieved The recycling of organic matter, secondary metabolite has many advantages, such as multi-biological is active, thus will become novel organic The important trend of fertilizer.The development and application of organic foliar fertilizer be human health, food safety, green agriculture production it is important It ensures.Based on microbial fermentation processes, nutrition equilibrium, the compound organic foliar fertilizer with anti-disease activity are developed, will be become novel The important development direction of organic foliar fertilizer, correlative study have particularly important theory significance and real value.However, being so far Only, based on fermentation process preparation the still rare report of organic liquor fabric and application, related-art process urgently improve with it is perfect.
Summary of the invention
The invention aims to solve existing foliar fertilizer unbalanced nutrition, do not have the deficiencies of anti-disease activity, and mention The fermentation liquid of high lactic acid and high streptococcus lactis cellulose content is obtained using lactobacillus-fermented for a kind of, so by with a certain alkalinity Inorganic elements neutralizes, and forms the organic element substance for being more conducive to plant absorption, then by amino acid and trace element built mode, Obtain the preparation method of the comprehensive compound organic foliar fertilizer of nutrition.Prepared compound organic foliar fertilizer can be used for fruits and vegetables and plant Green, fruit retention, fresh adding, volume increase, disease-resistant is protected in journey, is enlarged afforested area during afforestation, is extended viewing period, raising plant drought cold-resistant energy Power.
One kind is that use can generate lactic acid and streptococcus lactis using the compound disease-resistant organic foliar fertilizer of height of fermentation method preparation Element lactobacillus-fermented preparation compound organic foliar fertilizer, rich in organic matter, specific organic metal element, nisin, Amino acid and microelement, can satisfy the nutritional need of different growing stage plant, while can be improved the disease-resistant, anti-of plant Drought, cold tolerance.
A kind of preparation method of high compound disease-resistant organic foliar fertilizer, is the lactic acid can generate lactic acid and nisin Bacterium adds the potassium element of high concentration into culture solution as fermentation strain, by changing condition of culture, prepares rich potassium and rich in cream The fermentation liquid of acid streptococci element, and then nutrition-balanced compound organic foliar fertilizer is obtained using preparation method.It includes following step It is rapid:
1) the step of lactic acid bacteria activates;
2) the step of prepared by lactic fermentation liquid;
3) the step of fermentation liquid compounds;
The lactobacillus bulgaricus (Lactobacillus of lactic acid and nisin can be generated Bulgaricus), Lactobacillus casei (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus Rhamnosus), lactobacillus plantarum (Lactobacillus plantarum) and streptococcus thermophilus (Streptococcus Thermophilus composite fermentation bacterial strain, activation condition are as follows: by each bacterial strain streak inoculation and MRS solid medium (grape) are used as Sugar 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, phosphoric acid hydrogen two Potassium 0.2%, epsom salt 0.05%, four water manganese sulfates 0.025%, agar 2%, pH=6.2~6.5), 37 degree of cultures 24 are small When, the lactic acid bacteria culturers after being activated.
Streptococcus acidi lactici fermented solution preparation:
Each lactic acid bacteria culturers combined inoculation after activation is to modified MRS culture medium (glucose 2%, peptone 1%, beef Cream 1%, yeast extract 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.2~1%, epsom salt 0.05%, four water manganese sulfates 0.025%, pH=6.2~6.5), at 37-42 DEG C, cultivate 36~48 hours, obtain rich in cream The streptococcus acidi lactici fermented solution of acid and nisin.
Foliar fertilizer compounding:
In the streptococcus acidi lactici fermented solution rich in lactic acid and nisin, organic fertilizer compounding is carried out in the following proportions, is stirred Dissolution is mixed, compound organic foliar fertilizer finished product is obtained.
The compound organic foliar fertilizer of the method for the present invention preparation can be used in fruits and vegetables planting process protecting flower, fruit retention, enlarge afforested area, increase It produces, enlarge afforested area during afforestation, extend viewing period, improve plant drought cold tolerance etc..The specific method is as follows: by preparation Compound organic foliar fertilizer is diluted with water 200~400 times, and dilution is sprayed in plant leaf blade and stalk surface, every 10 days~ It sprays within 20 days once, plant yield-increasing can be promoted to increase income, maintain ornamental performance, it is inverse to arid, pest and disease damage, cold etc. to improve plant The tolerance in border.
The advantages of the present invention:
The method of the present invention, which has, operates convenient, environment-friendly and green, no coupling product, non-toxic ingredient generation, and product has property Stablize, nutrition equilibrium, be rich in the features such as biologically active polypeptide, the fertilizer efficiency of production efficiency and product can be effectively improved, to produce Raw good economic benefit and social benefit.
Detailed description of the invention
Fig. 1 is the technology path of high compound disease-resistant organic foliar fertilizer preparation.
The present invention will be described in detail with reference to the accompanying drawings and embodiments.
Specific embodiment
Embodiment one: the preparation and application of high compound disease-resistant organic foliar fertilizer
The technology path of high compound disease-resistant organic foliar fertilizer preparation is shown in attached drawing 1.
(1) prepared by streptococcus acidi lactici fermented solution
The lactic acid bacteria saved from -80 degree refrigerator glycerol tubes is activated.Lactic acid bacteria used include can generate lactic acid and Lactobacillus bulgaricus (Lactobacillus bulgaricus), the Lactobacillus casei of nisin (Lactobacillus casei), Lactobacillus rhamnosus (Lactobacillus rhamnosus), lactobacillus plantarum (Lactobacillus plantarum) and streptococcus thermophilus (Streptococcus thermophilus).Using above-mentioned multiple Combined bacteria strain carries out lactic fermentation liquid preparation.Activation condition are as follows: choose each bacterial strain from glycerol tube, streak inoculation and MRS solid Culture medium (glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.05%, four water manganese sulfates 0.025%, agar 2%, pH=6.2~ 6.5) it, cultivates 24 hours for 37 degree, the lactic acid bacteria culturers after being activated.
By each lactic acid bacteria culturers combined inoculation after activation to modified MRS culture medium (glucose 2%, peptone 1%, ox Meat extract 1%, yeast extract 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.2~1%, seven water sulfuric acid Magnesium 0.05%, four water manganese sulfates 0.025%, pH=6.2~6.5), at 37-42 DEG C, shaking table culture 36~48 hours, obtain Streptococcus acidi lactici fermented solution rich in lactic acid and nisin.
(2) fermentation liquid compounds
In the streptococcus acidi lactici fermented solution rich in lactic acid and nisin, organic fertilizer compounding is carried out in the following proportions: hair Zymotic fluid 500mL, potassium hydroxide 60-80g, compound amino acid 100-120g, microelement 2-3g.First potassium hydroxide is dissolved in In 100mL water, it is slowly added to lactic acid after cooling, it is cooling.Then fermentation liquid is mixed with the potassium hydroxide solution that lactic acid neutralizes It is even.Compound amino acid, glutamic acid, microelement are successively added again.After stirring and dissolving, compound organic foliar fertilizer finished product is obtained.
(4) application of compound organic foliar fertilizer
The compound organic foliar fertilizer of the method for the present invention preparation can be used in fruits and vegetables planting process protecting flower, fruit retention, enlarge afforested area, increase It produces, enlarge afforested area during afforestation, extend viewing period, improve plant drought cold tolerance etc..The specific method is as follows:
The compound organic foliar fertilizer of preparation is diluted with water 200-400 times, dilution is sprayed in plant leaf blade and stalk Surface, sprayed primary every 10 days~20 days, plant yield-increasing can be promoted to increase income, maintain ornamental performance, improve plant to arid, The tolerance of the adverse circumstances such as pest and disease damage, cold.
By the actual use to ornamental flower and Pepper crops, prepare 2 basins respectively in the case where the same terms are planted and are cultivated Plant, and 2 basin plant is made to keep essentially identical in height, size even contained blade, color etc., using the side of comparison Formula, wherein a basin is as a comparison case, another basin uses product of the present invention to carry out spraying cultivation as embodiment.Through after a period of time Afterwards, the plant size of the ornamental flower and capsicum of embodiment, blade, situation of blooming are significantly better than that comparative example, can effectively mention The fertilizer efficiency of high efficiency and product.

Claims (7)

1. a kind of preparation method of high compound disease-resistant organic foliar fertilizer, this method comprises:
1) the step of lactic acid bacteria activates;
2) the step of prepared by lactic fermentation liquid;
3) the step of fermentation liquid compounds;
Lactic acid bacteria used in it includes: lactobacillus bulgaricus Lactobacillus bulgaricus, Lactobacillus casei Lactobacillus casei, Lactobacillus rhamnosus Lactobacillus rhamnosus, lactobacillus plantarum Lactobacillus plantarum and streptococcus thermophilus Streptococcus thermophilus;Fermentation liquid compounding is past Be added in streptococcus acidi lactici fermented solution: compound organic foliar fertilizer is prepared in potassium hydroxide, compound organic matter, microelement.
2. the preparation method of the compound disease-resistant organic foliar fertilizer of height according to claim 1, it is characterised in that: in step 1) Activation condition are as follows: cultivate each bacterial strain streak inoculation and MRS solid medium 24 hours at 37 degree, the lactic acid bacteria after being activated Strain;Wherein MRS solid medium includes consisting of ingredient: glucose 2%, peptone 1%, beef extract 1%, yeast extract Object 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, dipotassium hydrogen phosphate 0.2%, epsom salt 0.05%, four water manganese sulfates 0.025%, agar 2%, pH=6.2~6.5.
3. the preparation method of the compound disease-resistant organic foliar fertilizer of height according to claim 1, it is characterised in that: step 2) be by To modified MRS culture medium at 37-42 DEG C, shaking table culture 36~48 is small for each lactic acid bacteria culturers combined inoculation after step 1) activation When, obtain the streptococcus acidi lactici fermented solution rich in lactic acid and nisin;Wherein modified MRS culture medium include consisting of at Point: glucose 2%, peptone 1%, beef extract 1%, yeast extract 0.5%, ammonium citrate 0.2%, sodium acetate 0.5%, phosphorus Sour hydrogen dipotassium 0.2~1%, epsom salt 0.05%, four water manganese sulfates 0.025%, pH=6.2~6.5.
4. the preparation method of the compound disease-resistant organic foliar fertilizer of height according to claim 1, it is characterised in that: pressed in step 3) Following ratio carries out organic fertilizer compounding: fermentation liquid 500mL, potassium hydroxide 60-80g, compound amino acid 100-120g, microelement 2-3g after stirring and dissolving, obtains compound organic foliar fertilizer finished product.
5. the preparation method of the compound disease-resistant organic foliar fertilizer of height according to claim 1-4, it is characterised in that: benefit With the organic acid of fermentation liquid itself, the organic metal element of a variety of high concentrations is introduced in existing compound organic foliar fertilizer, The compound organic foliar fertilizer of gained contains but is not limited to organic potassium, organic calcium.
6. the preparation method of the compound disease-resistant organic foliar fertilizer of height according to claim 5, it is characterised in that: be prepared multiple Organic foliar fertilizer is closed rich in organic acid, potassium ion, phosphate radical, micro elements by plants, amino acid, nisin.
7. the application of the compound disease-resistant organic foliar fertilizer of height described in claim 1, it is characterised in that: by compound organic leaf of preparation Leaven is diluted with water 200-400 times, and dilution is sprayed in plant leaf blade and stalk surface, sprays one every 10 days~20 days It is secondary.
CN201910010560.8A 2019-01-07 2019-01-07 A kind of preparation method and applications of high compound disease-resistant organic foliar fertilizer Pending CN109593010A (en)

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CN111567261A (en) * 2020-05-14 2020-08-25 中国科学院沈阳应用生态研究所 Plant toner and application and use method thereof
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Application publication date: 20190409