CN103992191B - A kind of complex biological bacterial manure - Google Patents
A kind of complex biological bacterial manure Download PDFInfo
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- CN103992191B CN103992191B CN201410244060.8A CN201410244060A CN103992191B CN 103992191 B CN103992191 B CN 103992191B CN 201410244060 A CN201410244060 A CN 201410244060A CN 103992191 B CN103992191 B CN 103992191B
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Abstract
The invention belongs to foliage fertilizer field, disclose a kind of complex biological bacterial manure, this bacterial manure is by waste material of edible mushroom vat liquor 30-40 part, and dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part and amine DA-6 1-2 part are prepared from.The invention also discloses the preparation method of above-mentioned bacterial manure.Bacterial manure of the present invention has the advantages such as volume increase, disease-resistant, environmental protection.
Description
Technical field
The present invention relates to foliage fertilizer technical field, be specifically related to a kind of complex biological bacterial manure and preparation method thereof.
Background technology
Foliar applied fertilizer is a kind of measures of fertilizer in agriculture production, under rhizosphere fertilization can not meet the prerequisite of high grade and yield of crops demand completely, come supplementary nutrient, regulation and control plant growth often through foliage-spray nutritive element or active substance, increase crop yield and oil recovery enhancement.Supplementing as soil application, leaf dressing has that consumption is few, efficiency is high, nutrition absorption transforms fast feature, and nutrient can also be prevented to be fixed at soil, and particularly the trace element such as iron, manganese, copper, zinc, is thus widely used in production estimation.
Foliage fertilizer is the general designation of the fertilizer being provided nutritive substance by blade for crop, by functional classification, and the nutritious type foliage fertilizer of current foliage fertilizer main Types, adjustment type foliage fertilizer, biotype foliage fertilizer, single type microelement foliage fertilizer, composite foliage fertilizer etc.Along with the development of foliage fertilizer application, composite multifunction changes into the direction into foliage fertilizer development now.Composite multifunction type foliage fertilizer is not only containing the nutritive element needed for plant, also containing biologically active substance amino acid, plant-growth regulator etc., the pest-resistant material of different sorts diseases prevention can also be added according to the actual needs of pest control treatment simultaneously, thus reach the multiple action effects such as extra-nutrition, growth regulation, raising output, oil recovery enhancement, controlling disease.
Microorganism foliage fertilizer is that spraying such foliage fertilizer can stimulate plant growth containing microbe and metabolite thereof in foliage fertilizer, promotes crop metabolism, alleviates and prevent the generation of disease and pest.The present invention relates to a kind of novel microorganism foliage fertilizer, utilize natural nutrient substance and fermented liquid by functional bacteria to work in coordination, reach promote plant growth, disease-resistant, volume increase object.
Summary of the invention
The object of the present invention is to provide the complex biological bacterial manure and preparation method thereof of a kind of volume increase, disease-resistant, environmental protection.
For achieving the above object, the present invention is by the following technical solutions:
A kind of complex biological bacterial manure, it is prepared from by the raw material of following weight part:
Waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part, amine DA-6 1-2 part;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
Cleaned up by the clout (comprising mushroom pin, broken mushroom etc.) that fresh edible mushrooms is plucked or the course of processing produces, the ratio by weight 1:1 adds water, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Seeding tank is adopted to ferment respectively respectively trichoderma harzianum and subtilis, after having fermented, concentration>=3 × 10 of trichoderma harziarum fermented liquid
8individual/ml, concentration>=1 × 10 in fermentation of bacillus subtilis liquid
9individual/ml; 1:1 is by trichoderma harziarum and the mixing of fermentation of bacillus subtilis liquid by volume, and stir obtained mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part and amine DA-6 1-2 part mix, and to obtain final product.
The fermentation of bacillus subtilis liquid used in biological organic fertilizer of the present invention, is obtained by subtilis (Bacillussubtilis) FG01 fermentation.Subtilis (Bacillussubtilis) FG01 carries out preservation, preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC: CGMCCNo.8082; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The trichoderma harziarum fermented liquid used in biological organic fertilizer of the present invention, is obtained by trichoderma harziarum (Trichodermaharzianum) FG02 fermentation.Trichoderma harziarum (Trichodermaharzianum) FG02 carries out preservation, preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC: CGMCCNo.8081; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Bacterial manure of the present invention is foliage fertilizer, and concrete method of application is: after plant-growth three leaves or the critical period of growing be watered dilution 500 times by every 100mL, 7-15 days sprinkling blade faces once, leaf pros and cons even spraying, with blade face have water droplet drip for degree.
The present invention is applicable to the multiple kinds of crops such as vegetables, fruit tree, cash crop.
The beneficial effect that the present invention obtains mainly comprise following some:
The Edible mushroom processing clout used in bacterial manure of the present invention and dregs of beans are processing byproduct, discarded rate is higher, containing abundant nutrition and the biologically active substance such as amino acid, trace element, fungus polysaccharide, soybean polysaccharide in its vat liquor, both for plant provides nutrition, disease resistance again.The nutritive substances such as amino acid contained in Edible mushroom processing clout and dregs of beans vat liquor can make up mutually the deficiency of the other side, and a large amount of amino acid whose synergistic effects improve the utilization ratio of nutrient, and nutrition more comprehensively, is more conducive to absorption and the utilization of plant; Achieve simultaneously and turn waste into wealth.
Trichoderma harziarum (Trichodermaharzianum) FG02 that the present invention uses and subtilis (Bacillussubtilis) FG01 is the antagonistic strain filtered out from occurring in nature, all have that growth is fast, strong resistance, go down to posterity stable feature, ecological niche can be occupied rapidly and play and suppress the effect of growth of pathogenic bacteria, overcome part at the excellent antagonistic strain of laboratory performance often in the shortcoming that variable rate technology is not good.Two kinds of microorganisms of the present invention adopt rational proportion, by symbiosis Relationship With Proliferation, define a complexity and stable microecosystem, effectively prevention and elimination of disease and pests.
Bacterial manure of the present invention is foliage fertilizer, has the advantages such as volume increase, disease-resistant, environmental protection, and its preparation technology is simple, is applicable to suitability for industrialized production.
Embodiment
To the mode of specific embodiment be adopted to carry out detailed description to the present invention below, but should not be construed limitation of the present invention.
Embodiment 1
The acquisition of subtilis (Bacillussubtilis) FG01:
Gather the healthy cucumber plant rhizosphere soil of falling ill in serious plot from the cucumber fusarium axysporum of Luozhuang District, Shandong as test materials, from this pedotheque, bacteria distribution is carried out in laboratory, LB substratum carries out separating for several times purifying, obtains pure culture bacterium colony, then uv irradiating 1min.After this bacterium 30 DEG C cultivates 48h, thalline subcircular, in light yellow, bacterium colony is comparatively large, and raw or near middle raw in gemma position, gemma expands, without parasporal crystal, single appearance; Pcr amplification 16SrDNA, finding through comparison, is 99% with the sequence similarity of subtilis, therefore, by its called after subtilis (Bacillussubtilis) FG01.This bacterial strain carries out preservation, preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC: CGMCCNo.8082; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.The fermentation culture conditions of this bacterial strain is: substratum: wheat bran 2.8%, rice husk 1%, Semen Maydis powder 2.5%, dregs of beans 1.5%, magnesium sulfate 0.05%, ammonium sulfate 0.5%, culture temperature 25 DEG C, natural ph.
The acquisition of trichoderma harziarum (Trichodermaharzianum) FG02:
Gather the healthy grapevine seedling rhizosphere soil of falling ill in serious plot from the fruit white rot of grape of Luozhuang District, Shandong as test materials, from this pedotheque, bacteria distribution is carried out in laboratory, on TSM culture medium flat plate, even with the L shape spreading rod coating of sterilizing, the constant incubator being placed in 23-26 DEG C cultivates 2-3 days.Picking form is transferred on PDA flat board like single bacterium colony of Trichoderma carries out purifying cultivation, LB substratum carries out separating for several times purifying, obtain pure culture bacterium colony, cultivate one week, bacterium colony spider's thread shape is to ulotrichy, white, and surface is in light green, the back side is colourless, and the later stage is because sporulation quantity ambassador bacterium colony is in slightly green; Subsphaeroidal or the obovate of conidium, wall is smooth, light green, pcr amplification 16SrDNA, and finding through comparison, is 99% with the sequence similarity of trichoderma harziarum; This bacterial strain carries out preservation, preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center CGMCC: CGMCCNo.8081; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.The fermentation culture conditions of this bacterial strain is: culture temperature 25 DEG C, natural ph, substratum: 2.00% wheat bran, 1.00% soybean cake powder, 1.00% (NH4) 2SO4,0.25%MgSO
4.7H2O, 0.50%KH2PO4, all the other are water.
Embodiment 2
A kind of complex biological bacterial manure, it is prepared from by the raw material of following weight part:
Waste material of edible mushroom vat liquor 30 parts, dregs of beans vat liquor 30 parts, mixed bacteria liquid 5 parts, potassium fulvate 2 parts, amine DA-6 1 part;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
Cleaned up by the clout (comprising mushroom pin, broken mushroom etc.) that fresh edible mushrooms is plucked or the course of processing produces, the ratio by weight 1:1 adds water, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Seeding tank is adopted to ferment respectively respectively trichoderma harzianum FG02 and subtilis FG01, after having fermented, the concentration 5 × 10 of trichoderma harziarum fermented liquid
8individual/ml, the concentration 1 × 10 in fermentation of bacillus subtilis liquid
9individual/ml; 1:1 is by trichoderma harziarum and the mixing of fermentation of bacillus subtilis liquid by volume, and stir obtained mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix according to weight part, to obtain final product.
Embodiment 3
A kind of complex biological bacterial manure, it is prepared from by the raw material of following weight part:
Waste material of edible mushroom vat liquor 40 parts, dregs of beans vat liquor 40 parts, mixed bacteria liquid 10 parts, potassium fulvate 3 parts, amine DA-6 2 parts;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
Cleaned up by the clout (comprising mushroom pin, broken mushroom etc.) that fresh edible Xianggu-mushroom is plucked or the course of processing produces, the ratio by weight 1:1 adds water, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Seeding tank is adopted to ferment respectively respectively trichoderma harzianum and subtilis, after having fermented, the concentration 5 × 10 of trichoderma harziarum fermented liquid
8individual/ml, the concentration 2 × 10 in fermentation of bacillus subtilis liquid
9individual/ml; 1:1 is by trichoderma harziarum and the mixing of fermentation of bacillus subtilis liquid by volume, and stir obtained mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix, and to obtain final product.
Embodiment 4
The manure trial of bacterial manure of the present invention:
One. for Huangguan Pear:
Bacterial manure prepared by the application embodiment of the present invention 1, on the impact of Huangguan Pear Yield and qualities, is chosen life in 10 years, Huangguan Pear fruit tree that growing way is consistent, is evenly sprayed blade face respectively in sproutings, florescence, young fruit period, expanding stage and painted phase, to spray equivalent clear water for contrasting.Take identical control measures after using, during fruit maturation, 10 fruits are adopted in each process, observe fruit yield and quality; Result, as shown in table 1,2, can increase Huangguan Pear single fruit weight and proportion after using this product, improves fruit quality.
Table 1 the present invention is on the impact of Huangguan Pear single fruit weight and proportion
| Process | Single fruit weight/(g/) | Proportion/(g/mL) |
| Test group | 306 | 1.14 |
| Control group | 262 | 1.06 |
Table 2 the present invention is on the impact of Huangguan Pear fruit quality
Two. for tomato:
Microbial inoculum prepared by the embodiment of the present invention 2 is on the impact of tomato yield and quality.Test group uses invention formulation, and to spray equivalent clear water for contrast, tomato variety is that gingko is rich by force, line-spacing 35cm, spacing in the rows 30cm, ridge height 15cm, Routine Management.Result as shown in Table 3, 4, can increase tomato yield after using this product, shortens Time To Market, strengthens tomato resistance against diseases, can also improve fruit quality, increases the economic worth of tamato fruit.
Table 3 the present invention is on the impact of tomato yield, collection period and disease resistance
Table 4 the present invention is on the impact of Quality of Tomato Fruit
Three. for cucumber:
The embodiment of the present invention 1 bacterial manure is on the impact of cucumber yield and quality.Spring plastic tent cucumber experimental cultivar is Tianjin spring No. 3, adopts high furrow ridge culture, and wide-and narrow-row is cultivated, plot area 10m
2this product of foliage-spray (test group 1) and commercially available Kang Piao foliage fertilizer (lion horse is green) (test group 2), connect spray 3 times, with foliage-spray clear water for contrast, measure its early yield (contrast reaches and begins to receive the output in after date 15d), every community is got 5 strains and is observed increment in its 15d.Result as illustrated in tables 5-6, can promote cucumber growth after using this product, strengthens cucumber resistance against diseases, improves cucumber yield and product quality.Indices is all better than or is not less than commercially available conventional foliage fertilizer.
Table 5 the present invention is on the impact of cucumber growth and output
Table 6 the present invention is on the impact of cucumber quality
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (1)
1. a complex biological bacterial manure, it is prepared from by the raw material of following weight part:
Waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part, amine DA-6 1-2 part;
itspreparation method comprises the steps:
1) preparation of waste material of edible mushroom vat liquor:
Cleaned up by the clout that fresh edible mushrooms is plucked or the course of processing produces, the ratio by weight 1:1 adds water, and 80 DEG C are extracted 1h, filter, get supernatant liquor and be waste material of edible mushroom vat liquor;
2) preparation of dregs of beans vat liquor:
Dregs of beans and water are mixed in the ratio of 1:1, stir, 80 DEG C are extracted 1h, filter, get supernatant liquor and be dregs of beans vat liquor;
3) preparation of mixed bacteria liquid:
Seeding tank is adopted to ferment respectively respectively trichoderma harzianum and subtilis, after having fermented, concentration>=3 × 10 of trichoderma harziarum fermented liquid
8individual/ml, concentration>=1 × 10 in fermentation of bacillus subtilis liquid
9individual/ml; 1:1 is by trichoderma harziarum and the mixing of fermentation of bacillus subtilis liquid by volume, and stir obtained mixed bacteria liquid;
4) preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix according to weight part, to obtain final product;
Described subtilis is subtilis (Bacillussubtilis) FG01, and preserving number is: CGMCCNo.8082; Described trichoderma harziarum is trichoderma harziarum (Trichodermaharzianum) FG02, and preserving number is: CGMCCNo.8081.
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| CN104446676B (en) * | 2014-12-24 | 2017-01-11 | 湖南省食用菌研究所 | Method for preparing amino acid water-soluble fertilizer from mushroom residue extract |
| CN105169446B (en) * | 2015-07-27 | 2017-03-15 | 河南越宝生物科技有限公司 | A kind of organic waste deodorizer |
| CN107371669A (en) * | 2016-11-16 | 2017-11-24 | 田阳农瑞德种苗专业合作社 | A kind of cultural method of organic vegetable |
| CN108207493B (en) * | 2018-02-09 | 2020-01-03 | 山东省农业科学院农业资源与环境研究所 | Straw rotting type edible fungus liquid strain culture medium, preparation method and application |
| CN108929166A (en) * | 2018-08-14 | 2018-12-04 | 上海绿乐生物科技有限公司 | A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism |
| CN109169129B (en) * | 2018-09-12 | 2021-06-08 | 吉林冠州生物科技有限公司 | Method for cultivating peony for paeonia ostii oil in Yanbian area |
| CN109485502A (en) * | 2018-11-29 | 2019-03-19 | 镇江贝思特有机活性肥料有限公司 | A kind of excitation crop forms the preparation of water soluble fertilizer dilution and its application method of antibody |
| CN111066790A (en) * | 2019-11-06 | 2020-04-28 | 广东植物龙生物技术股份有限公司 | Plant growth regulator combining diethyl aminoethyl hexanoate and bacillus altitudinis fungus-containing metabolites |
| CN113929531A (en) * | 2021-11-19 | 2022-01-14 | 淮滨县以琳有机肥料有限公司 | Fertilizer based on edible fungus waste material and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041601A (en) * | 2007-04-30 | 2007-09-26 | 山东省农业科学院土壤肥料研究所 | Foliage fertilizer containing glossy ganoderma leach and preparation method thereof |
| CN103342604A (en) * | 2013-07-10 | 2013-10-09 | 湖南泰谷生物科技股份有限公司 | Bio-control bacterium foliage fertilizer, and preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101041601A (en) * | 2007-04-30 | 2007-09-26 | 山东省农业科学院土壤肥料研究所 | Foliage fertilizer containing glossy ganoderma leach and preparation method thereof |
| CN103342604A (en) * | 2013-07-10 | 2013-10-09 | 湖南泰谷生物科技股份有限公司 | Bio-control bacterium foliage fertilizer, and preparation method and application thereof |
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Effective date of registration: 20190819 Address after: 162657 Gaotaizi Village, Gaotaizi Office, Zhalantun City, Hulunbeir City, Inner Mongolia Autonomous Region Patentee after: Inner Mongolia Wofeng Agricultural Development Co., Ltd. Address before: 277724 South of Qianyongquan Village, Zhuangwu Town, Lanling County, Linyi City, Shandong Province Patentee before: Shandong Fuguo Biotechnology Co., Ltd. |