CN103992191A - Compound biological bacterial fertilizer - Google Patents

Compound biological bacterial fertilizer Download PDF

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Publication number
CN103992191A
CN103992191A CN201410244060.8A CN201410244060A CN103992191A CN 103992191 A CN103992191 A CN 103992191A CN 201410244060 A CN201410244060 A CN 201410244060A CN 103992191 A CN103992191 A CN 103992191A
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vat liquor
preparation
dregs
beans
liquid
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CN103992191B (en
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尹成亮
李宪明
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Inner Mongolia Wofeng Agricultural Development Co., Ltd.
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SHANDONG FUGUO BIOTECHNOLOGY CO Ltd
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Abstract

The invention belongs to the field of foliar fertilizers, and discloses a compound biological bacterial fertilizer. The bacterial fertilizer is prepared from 30-40 parts of edible fungi waste extract, 30-40 parts of bean pulp extract, 5-10 parts of mixed bacterial solution, 2-3 parts of potassium fulvate and 1-2 parts of diethyl aminoethyl hexanoate. The invention further discloses a preparation method of the bacterial fertilizer. The bacterial fertilizer has the advantages of yield increase, disease resistance, environmental friendliness and the like.

Description

A kind of complex biological bacterial manure
 
Technical field
The present invention relates to foliage fertilizer technical field, be specifically related to a kind of complex biological bacterial manure and preparation method thereof.
Background technology
Foliar applied fertilizer is a kind of measures of fertilizer in agriculture production, can not meet completely under the prerequisite of high grade and yield of crops demand at rhizosphere fertilization, often supplement nutrient, regulation and control plant growth by foliage-spray nutritive element or active substance, increase crop yield and improve quality.As supplementing of soil application, leaf dressing has that consumption is few, efficiency is high, nutrition absorption transforms fast feature, can also prevent that nutrient is fixed at soil, the particularly trace element such as iron, manganese, copper, zinc, thereby be widely used in farm crop produce.
Foliage fertilizer is to provide the general designation of the fertilizer of nutritive substance by blade for crop, by functional classification, and the nutritious type foliage fertilizer of current foliage fertilizer main Types, adjustment type foliage fertilizer, biotype foliage fertilizer, single type microelement foliage fertilizer, composite foliage fertilizer etc.Along with the development of foliage fertilizer application, composite multifunction changes into the direction into foliage fertilizer development now.Composite multifunction type foliage fertilizer not only contains the required nutritive element of plant, also contain biologically active substance amino acid, plant-growth regulator etc., simultaneously can also add the pest-resistant material of different sorts diseases prevention according to the actual needs of pest control treatment, thereby reach extra-nutrition, growth regulation, raising output, improve quality, prevent and treat the multiple action effects such as disease.
Microorganism foliage fertilizer is that foliage fertilizer contains microbe and metabolite thereof, and spraying such foliage fertilizer can stimulate plant growth, promotes crop metabolism, alleviates and prevent the generation of disease and pest.The present invention relates to a kind of novel microorganism foliage fertilizer, utilize natural nutrient substance and fermented liquid by functional bacteria to work in coordination, reach promote plant growth, disease-resistant, volume increase object.
Summary of the invention
The object of the present invention is to provide complex biological bacterial manure of a kind of volume increase, disease-resistant, environmental protection and preparation method thereof.
For achieving the above object, the present invention is by the following technical solutions:
A kind of complex biological bacterial manure, its raw material by following weight part is prepared from:
Waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part, amine DA-6 1-2 part;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
The clout (comprising mushroom pin, broken mushroom etc.) that fresh edible mushrooms is plucked or the course of processing produces is cleaned up, add water by weight the ratio of 1:1,80 DEG C are extracted 1h, filter, and get supernatant liquor and are waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, and get supernatant liquor and are dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Adopt respectively seeding tank to ferment respectively trichoderma harzianum and subtilis, after having fermented, concentration>=3 × 10 of trichoderma harziarum fermented liquid 8individual/ml, concentration>=1 × 10 in fermentation of bacillus subtilis liquid 9individual/ml; 1:1 mixes trichoderma harziarum and fermentation of bacillus subtilis liquid by volume, stirs and makes mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part and amine DA-6 1-2 part mix, and to obtain final product.
The fermentation of bacillus subtilis liquid using in biological organic fertilizer of the present invention, is made by subtilis (Bacillus subtilis) FG01 fermentation.Subtilis (Bacillus subtilis) FG01 carries out preservation, preserving number at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms: CGMCC No.8082; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The trichoderma harziarum fermented liquid using in biological organic fertilizer of the present invention, is made by trichoderma harziarum (Trichoderma harzianum) FG02 fermentation.Trichoderma harziarum (Trichoderma harzianum) FG02 carries out preservation, preserving number at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms: CGMCC No.8081; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.
Bacterial manure of the present invention is foliage fertilizer, and concrete method of application is: after three leaves of plant-growth or the critical period of growing be watered 500 times of dilutions by every 100mL, 7-15 days sprinkling blade faces once, leaf pros and cons even spraying, having water droplet drippage taking blade face is degree.
The present invention is applicable to the multiple kinds of crops such as vegetables, fruit tree, cash crop.
The beneficial effect that the present invention obtains mainly comprise following some:
The Edible mushroom processing clout using in bacterial manure of the present invention and dregs of beans are processing byproduct, discarded rate is higher, in its vat liquor, contain nutrition and the biologically active substances such as abundant amino acid, trace element, fungus polysaccharide, soybean polysaccharide, both for plant provides nutrition, disease resistance again.In Edible mushroom processing clout and dregs of beans vat liquor, the nutritive substance such as contained amino acid can make up mutually the other side's deficiency, and a large amount of amino acid whose synergistic effects have improved the utilization ratio of nutrient, and nutrition more comprehensively, is more conducive to absorption and the utilization of plant; Realize and having turned waste into wealth simultaneously.
Trichoderma harziarum (Trichoderma harzianum) FG02 and subtilis (Bacillus subtilis) FG01 that the present invention uses are the antagonistic strains filtering out from occurring in nature, all have that growth is fast, strong resistance, stable feature goes down to posterity, can occupy rapidly the effect that ecological niche performance suppress pathogenic bacteria growth, overcome part and showed the shortcoming that good antagonistic strain is often performed poor in field in laboratory.Two kinds of microorganisms of the present invention adopt rational proportion, by symbiosis Relationship With Proliferation, have formed a complexity and stable microecosystem, effectively prevention and elimination of disease and pests.
Bacterial manure of the present invention is foliage fertilizer, has the advantages such as volume increase, disease-resistant, environmental protection, and its preparation technology is simple, is applicable to suitability for industrialized production.
Embodiment
Below the mode that adopts specific embodiment is carried out to detailed description to the present invention, but should not be construed limitation of the present invention.
Embodiment 1
The acquisition of subtilis (Bacillus subtilis) FG01:
Gather the healthy cucumber plant rhizosphere soil of falling ill in serious plot from the cucumber fusarium axysporum of Luozhuang District, Shandong as test materials, in laboratory, from this pedotheque, carry out bacterium separation, on LB substratum, carry out separating for several times purifying, obtain pure culture bacterium colony, then uv irradiating 1min.After 30 DEG C of cultivation 48h of this bacterium, thalline subcircular, is light yellow, and bacterium colony is larger, and raw or near middle raw in gemma position, gemma expands, without parasporal crystal, and single appearance; Pcr amplification 16S rDNA, through comparison find, with the sequence similarity of subtilis be 99%, therefore, by its called after subtilis (Bacillus subtilis) FG01.This bacterial strain carries out preservation, preserving number at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms: CGMCC No.8082; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.The fermentation culture conditions of this bacterial strain is: substratum: wheat bran 2.8%, rice husk 1%, Semen Maydis powder 2.5%, dregs of beans 1.5%, magnesium sulfate 0.05%, ammonium sulfate 0.5%, 25 DEG C of culture temperature, natural pH value.
The acquisition of trichoderma harziarum (Trichoderma harzianum) FG02:
Gather the healthy grapevine seedling rhizosphere soil of falling ill in serious plot from the fruit white rot of grape of Luozhuang District, Shandong as test materials, in laboratory, from this pedotheque, carry out bacterium separation, on TSM culture medium flat plate, even with the L shaped spreading rod coating of sterilizing, the constant incubator that is placed in 23-26 DEG C is cultivated 2-3 days.Picking form is transferred to and on PDA flat board, carries out purifying cultivation like single bacterium colony of Trichoderma, on LB substratum, carry out separating for several times purifying, obtain pure culture bacterium colony, cultivate one week, bacterium colony spider's thread shape is to ulotrichy, white, and surface is light green, the back side is colourless, and the later stage is because sporulation quantity ambassador bacterium colony is slightly green; Subsphaeroidal or the obovate of conidium, wall is smooth, light green, pcr amplification 16S rDNA, finds through comparison, with the sequence similarity of trichoderma harziarum be 99%; This bacterial strain carries out preservation, preserving number at the common micro-organisms center C GMCC of China Committee for Culture Collection of Microorganisms: CGMCC No.8081; The preservation time: on 08 28th, 2013; Preservation address: China Committee for Culture Collection of Microorganisms's common micro-organisms center.The fermentation culture conditions of this bacterial strain is: 25 DEG C of culture temperature, natural pH value, substratum: 2.00% wheat bran, 1.00% soybean cake powder, 1.00% (NH4) 2SO4,0.25%MgSO 4.7H2O, 0.50%KH2PO4, all the other are water.
Embodiment 2
A kind of complex biological bacterial manure, its raw material by following weight part is prepared from:
30 parts of waste material of edible mushroom vat liquors, 30 parts of dregs of beans vat liquors, 5 parts of mixed bacteria liquids, 2 parts of potassium fulvates, 1 part of amine DA-6;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
The clout (comprising mushroom pin, broken mushroom etc.) that fresh edible mushrooms is plucked or the course of processing produces is cleaned up, add water by weight the ratio of 1:1,80 DEG C are extracted 1h, filter, and get supernatant liquor and are waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, and get supernatant liquor and are dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Adopt respectively seeding tank to ferment respectively trichoderma harzianum FG02 and subtilis FG01, after having fermented, the concentration 5 × 10 of trichoderma harziarum fermented liquid 8individual/ml, the concentration 1 × 10 in fermentation of bacillus subtilis liquid 9individual/ml; 1:1 mixes trichoderma harziarum and fermentation of bacillus subtilis liquid by volume, stirs and makes mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix according to weight part, to obtain final product.
Embodiment 3
A kind of complex biological bacterial manure, its raw material by following weight part is prepared from:
40 parts of waste material of edible mushroom vat liquors, 40 parts of dregs of beans vat liquors, 10 parts of mixed bacteria liquids, 3 parts of potassium fulvates, 2 parts of amine DA-6s;
The preparation method of above-mentioned bio-bacterial manure comprises the steps:
The preparation of waste material of edible mushroom vat liquor:
The clout (comprising mushroom pin, broken mushroom etc.) that fresh edible Xianggu-mushroom is plucked or the course of processing produces is cleaned up, add water by weight the ratio of 1:1,80 DEG C are extracted 1h, filter, and get supernatant liquor and are waste material of edible mushroom vat liquor;
The preparation of dregs of beans vat liquor:
Fresh, qualified dregs of beans adds water in the ratio of 1:1, stirs, and 80 DEG C are extracted 1h, filter, and get supernatant liquor and are dregs of beans vat liquor;
The preparation of mixed bacteria liquid:
Adopt respectively seeding tank to ferment respectively trichoderma harzianum and subtilis, after having fermented, the concentration 5 × 10 of trichoderma harziarum fermented liquid 8individual/ml, the concentration 2 × 10 in fermentation of bacillus subtilis liquid 9individual/ml; 1:1 mixes trichoderma harziarum and fermentation of bacillus subtilis liquid by volume, stirs and makes mixed bacteria liquid;
The preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix, and to obtain final product.
Embodiment 4
The manure trial of bacterial manure of the present invention:
One. taking Huangguan Pear as example:
The impact of the bacterial manure prepared of the application embodiment of the present invention 1 on Huangguan Pear output and quality, chooses life in 10 years, Huangguan Pear fruit tree that growing way is consistent, evenly sprays respectively blade face, to spray equivalent clear water as contrast in sprouting, florescence, young fruit period, expanding stage and painted phase.After use, take identical control measures, when fruit maturation, 10 fruits are adopted in each processing, and fruit yield and quality are observed; Result, as shown in table 1,2, can increase Huangguan Pear single fruit weight and proportion after using this product, improves fruit quality.
 
The impact of table 1 the present invention on Huangguan Pear single fruit weight and proportion
Process Single fruit weight/(g/) Proportion/(g/mL)
Test group 306 1.14
Control group 262 1.06
The impact of table 2 the present invention on Huangguan Pear fruit quality
Two. taking tomato as example:
Microbial inoculum prepared by the embodiment of the present invention 2 impact on tomato yield and quality.Test group is used preparation of the present invention, and to spray equivalent clear water as contrast, tomato variety is that gingko is rich by force, line-spacing 35cm, spacing in the rows 30cm, the high 15cm in ridge, Routine Management.Result as shown in Table 3, 4, can increase tomato yield after using this product, shortens Time To Market, strengthens tomato resistance against diseases, can also improve fruit quality, increases the economic worth of tamato fruit.
The impact of table 3 the present invention on tomato yield, collection period and disease resistance
The impact of table 4 the present invention on Quality of Tomato Fruit
Three. taking cucumber as example:
The impact of the embodiment of the present invention 1 bacterial manure on cucumber yield and quality.Spring plastic tent cucumber experimental cultivar is Tianjin spring No. 3, adopts high furrow ridge culture, wide-and narrow-row cultivation, community area 10m 2this product of foliage-spray (test group 1) and commercially available Kang Piao foliage fertilizer (lion horse is green) (test group 2), connect and spray 3 times, taking foliage-spray clear water as contrast, measure its early yield (contrast reaches and begins to receive the output in after date 15 d), every community is got 5 strains and is observed the increment in its 15 d.Result, as shown in table 5-6, can promote cucumber growth after using this product, strengthens cucumber resistance against diseases, improves cucumber yield and product quality.On indices, be all better than or be not less than commercially available conventional foliage fertilizer.
 
The impact of table 5 the present invention on cucumber growth and output
The impact of table 6 the present invention on cucumber quality
Although, above with general explanation and embodiment, this case being done to detailed explanation, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, the amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.

Claims (3)

1. a complex biological bacterial manure, its raw material by following weight part is prepared from:
Waste material of edible mushroom vat liquor 30-40 part, dregs of beans vat liquor 30-40 part, mixed bacteria liquid 5-10 part, potassium fulvate 2-3 part, amine DA-6 1-2 part.
2. bacterial manure as claimed in claim 1, is characterized in that, the preparation method of described fertilizer comprises the steps:
1) preparation of waste material of edible mushroom vat liquor:
The clout (comprising mushroom pin, broken mushroom etc.) that fresh edible mushrooms is plucked or the course of processing produces is cleaned up, add water by weight the ratio of 1:1,80 DEG C are extracted 1h, filter, and get supernatant liquor and are waste material of edible mushroom vat liquor;
2) preparation of dregs of beans vat liquor:
Dregs of beans and water are mixed in the ratio of 1:1, stir, 80 DEG C are extracted 1h, filter, and get supernatant liquor and are dregs of beans vat liquor;
3) preparation of mixed bacteria liquid:
Adopt respectively seeding tank to ferment respectively trichoderma harzianum and subtilis, after having fermented, concentration>=3 × 10 of trichoderma harziarum fermented liquid 8individual/ml, concentration>=1 × 10 in fermentation of bacillus subtilis liquid 9individual/ml; 1:1 mixes trichoderma harziarum and fermentation of bacillus subtilis liquid by volume, stirs and makes mixed bacteria liquid;
4) preparation of bio-bacterial manure:
By waste material of edible mushroom vat liquor, dregs of beans vat liquor, mixed bacteria liquid, potassium fulvate and amine DA-6 mix according to weight part, to obtain final product;
Described subtilis is subtilis (Bacillus subtilis) FG01, and preserving number is: CGMCC No.8082; Described trichoderma harziarum is trichoderma harziarum (Trichoderma harzianum) FG02, and preserving number is: CGMCC No.8081.
Bacterial manure described in claim 1 or 2 increasing crop yield or disease-resistant in application.
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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104446676A (en) * 2014-12-24 2015-03-25 湖南省春华生物科技有限公司 Method for preparing amino acid water-soluble fertilizer from mushroom residue extract
CN107050492A (en) * 2015-07-27 2017-08-18 合肥智慧殿投资管理有限公司 A kind of organic waste deodorant
CN107371669A (en) * 2016-11-16 2017-11-24 田阳农瑞德种苗专业合作社 A kind of cultural method of organic vegetable
CN108207493A (en) * 2018-02-09 2018-06-29 山东省农业科学院农业资源与环境研究所 A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application
CN108929166A (en) * 2018-08-14 2018-12-04 上海绿乐生物科技有限公司 A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism
CN109169129A (en) * 2018-09-12 2019-01-11 吉林冠州生物科技有限公司 Prolong the cultural method that the red oil of Region In Yanbian phoenix uses tree peony
CN109485502A (en) * 2018-11-29 2019-03-19 镇江贝思特有机活性肥料有限公司 A kind of excitation crop forms the preparation of water soluble fertilizer dilution and its application method of antibody
CN111066790A (en) * 2019-11-06 2020-04-28 广东植物龙生物技术股份有限公司 Plant growth regulator combining diethyl aminoethyl hexanoate and bacillus altitudinis fungus-containing metabolites
CN113929531A (en) * 2021-11-19 2022-01-14 淮滨县以琳有机肥料有限公司 Fertilizer based on edible fungus waste material and preparation method and application thereof

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* Cited by examiner, † Cited by third party
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CN101041601A (en) * 2007-04-30 2007-09-26 山东省农业科学院土壤肥料研究所 Foliage fertilizer containing glossy ganoderma leach and preparation method thereof
CN103342604A (en) * 2013-07-10 2013-10-09 湖南泰谷生物科技股份有限公司 Bio-control bacterium foliage fertilizer, and preparation method and application thereof

Patent Citations (2)

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Publication number Priority date Publication date Assignee Title
CN101041601A (en) * 2007-04-30 2007-09-26 山东省农业科学院土壤肥料研究所 Foliage fertilizer containing glossy ganoderma leach and preparation method thereof
CN103342604A (en) * 2013-07-10 2013-10-09 湖南泰谷生物科技股份有限公司 Bio-control bacterium foliage fertilizer, and preparation method and application thereof

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104446676A (en) * 2014-12-24 2015-03-25 湖南省春华生物科技有限公司 Method for preparing amino acid water-soluble fertilizer from mushroom residue extract
CN107050492A (en) * 2015-07-27 2017-08-18 合肥智慧殿投资管理有限公司 A kind of organic waste deodorant
CN107050492B (en) * 2015-07-27 2020-02-18 深圳璐璐兴家居用品有限公司 Organic garbage deodorant
CN107371669A (en) * 2016-11-16 2017-11-24 田阳农瑞德种苗专业合作社 A kind of cultural method of organic vegetable
CN108207493A (en) * 2018-02-09 2018-06-29 山东省农业科学院农业资源与环境研究所 A kind of grass corruption type edible fungus liquid culture growth medium, preparation method and application
CN108929166A (en) * 2018-08-14 2018-12-04 上海绿乐生物科技有限公司 A kind of a great number of elements Water soluble fertilizer and its production method containing microorganism
CN109169129A (en) * 2018-09-12 2019-01-11 吉林冠州生物科技有限公司 Prolong the cultural method that the red oil of Region In Yanbian phoenix uses tree peony
CN109485502A (en) * 2018-11-29 2019-03-19 镇江贝思特有机活性肥料有限公司 A kind of excitation crop forms the preparation of water soluble fertilizer dilution and its application method of antibody
CN111066790A (en) * 2019-11-06 2020-04-28 广东植物龙生物技术股份有限公司 Plant growth regulator combining diethyl aminoethyl hexanoate and bacillus altitudinis fungus-containing metabolites
CN113929531A (en) * 2021-11-19 2022-01-14 淮滨县以琳有机肥料有限公司 Fertilizer based on edible fungus waste material and preparation method and application thereof

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Address before: 277724 South of Qianyongquan Village, Zhuangwu Town, Lanling County, Linyi City, Shandong Province

Patentee before: Shandong Fuguo Biotechnology Co., Ltd.