CN102910988A - Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer - Google Patents

Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer Download PDF

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CN102910988A
CN102910988A CN2012104635921A CN201210463592A CN102910988A CN 102910988 A CN102910988 A CN 102910988A CN 2012104635921 A CN2012104635921 A CN 2012104635921A CN 201210463592 A CN201210463592 A CN 201210463592A CN 102910988 A CN102910988 A CN 102910988A
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milliliter
bacterial fertilizer
microorganism liquid
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CN102910988B (en
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蒋常德
胡艳晖
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Popek International Ecological Technology (beijing) Co Ltd
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Abstract

The invention discloses a compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and a preparation method of the compound microorganism liquid bacterial fertilizer and belongs to the technical field of microorganisms. The bacterial fertilizer comprises raw materials of, by weight, 10-20 parts of paprika powders, 10-20 parts of chilli powders, 5-15 parts of ginkgo leaf powders, 5-15 parts of chinaberry powders, 5-15 parts of lightyellow sophora root powders, 20-40 parts of mashed garlic, 40-80 parts of molasses, 4-8 parts of soybean meal, 5-15 parts of calcium sulfate, 5-15 parts of magnesium chloride, 5-10 parts of salt, 5-15 parts of photosynthetic bacterium strains, 5-15 parts of saccharomycete strains, 5-15 parts of lactic acid bacterium strains, 5-15 parts of streptomyces jingyangensis strains, 5-15 parts of bacillus subtilis strains and 800 parts of water. According to the compound microorganism liquid bacterial fertilizer, probiotic floras which are screened and are capable of coexisting are used for fermenting plant leaching agents of pepper, chilli, ginkgo leaves, chinaberry, lightyellow sophora roots and garlic, the plant leaching agents are capable of expelling and killing the insect pests and harmful bacteria, the compound microorganism liquid bacterial fertilizer has the advantages of being high in content of live bacteria of probiotics, high in activities, high in stability, small in using amount of plant materials, fast in acting effects, non-toxic and environment-friendly, and has wide application prospects in agricultural planting.

Description

A kind of complex microorganism liquid bacterial fertilizer of preventing and treating plant pest and preparation method thereof
Technical field
The invention belongs to the microbial fertilizer technical field, be specifically related to a kind ofly can prevent and treat plant pest, replenish the calcium, mend multi-efficiency complex microorganism liquid bacterial fertilizers such as magnesium, fertilising and preparation method thereof.
Background technology
Use unreasonably for a long time chemical fertilizer, agricultural chemicals, cause the wasting of resources and production cost to increase, severe environmental pollution, agricultural product quality descends.The green barrier of international agriculture trade is that export of farm produce difficulty increases, and the peasant faces the severe challenge that Higher output is not accompanied by a higher income.In recent years, along with the raising of environmental protection and health perception, the nationwide has started " green food " heat.Simultaneously, along with developing rapidly of foreign trade, also grow with each passing day to the demand of pollution-free food in the world market.Some developed countries take Green Trade Barrier in order to protect the agricultural from state.Namely a large amount of agricultural-food of chemical fertilizer and agricultural chemicals that use are kept outside of the door.Therefore, in agriculture production, has the Important Economic benefit, environmental benefit and social benefit be to open up new microorganism medicine fertilizer even not use chemical fertilizer and agricultural chemicals to reduce, produce the agricultural-food of high yield and high quality, improve international competitiveness, promote the Sustainable development of rural economy.But biological pesticide sterilant and the microbial-bacterial fertilizer of the development and production of Present Domestic institute can improve the immunizing power of plant, and the prevention physiological barrier is to reach the purpose of stable yield and high quality.But, promote in the growth can not with chemical fertilizer relatively, can not be directly and the agricultural chemicals comparison aspect curing the disease at desinsection.Before not entering agricultural modernization, the example that China also has a large amount of use vegetable Chinese herbal medicine materials to carry out the diseases prevention insect protected, but exist the vegetable Chinese herbal medicine material usage large, effect is single, the shortcomings such as time length weak point.
Summary of the invention
The objective of the invention is for the deficiencies in the prior art, biotechnology and Chinese Chinese traditional herbs technology are combined, provide that a kind of probiotics viable bacteria content is high, the vegetable Chinese herbal medicine material usage is few, active high, stability is high, action effect is fast, have the kill harmful bacterium, kill disease and insect, replenish the calcium, mend the complex microorganism liquid bacterial fertilizer of the multi-efficiencies such as magnesium, fertilising, rehabilitating soil, volume increase.Another object of the present invention provides the preparation method of this complex microorganism liquid bacterial fertilizer.
To achieve these goals, the technical scheme that the present invention adopts is: a kind of complex microorganism liquid bacterial fertilizer of preventing and treating plant pest is characterized in that the raw material for preparing this bacterial manure is by weight: zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 0-5 part, China tree powder 0-5 part, kuh-seng powder 0-5 part, salt 5-10 part, mashed garlic 20-40 part, greater than 6,000,000,000/milliliter photosynthetic bacterium 5-15 part, greater than 500,000,000/ml yeast bacterium 5-15 part, greater than 10,000,000,000/milliliter plant lactobacillus 5-15 part, greater than 100,000,000/milliliter Jingyang streptomycete 5-15 part, greater than 6,000,000,000/milliliter subtilis 5-15 part, molasses 40-80 part, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, 800 parts in water.
The preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest of the present invention:
⑴, in fermentor tank, add successively: 800 parts of molasses 40-80 parts, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 5-15 part, China tree powder 5-15 part, kuh-seng powder 5-15 part, salt 5-10 part, water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add mashed garlic 20-40 part, greater than 6,000,000,000/milliliter photosynthetic bacterium 5-15 part, greater than 500,000,000/ml yeast bacterium 5-15 part, greater than 10,000,000,000/milliliter plant lactobacillus 5-15 part, greater than 100,000,000/milliliter Jingyang streptomycete 5-15 part, greater than 6,000,000,000/milliliter subtilis 5-15 part;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.
Can adopt prior art at step ⑵ photosynthetic bacterium bacterial classification, yeast bacterial classification, lactic acid bacteria culturers, Jingyang streptomyces species, Bacillus subtilis strain, the invention provides following methods.
1., the preparation of milk-acid bacteria liquid bacterial classification
Take out the culture presevation pipe, draw flat board with the MRS solid medium and recover, 37 degree anaerobism were cultivated 48 hours.In 50 milliliters of MRS substratum of the single colony inoculation of picking, 37 degree anaerobism were cultivated 24 hours in incubator under flat board.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 10L, and 37 degree anaerobism were cultivated 20-28 hour, detected its bacterial concentration, and bacterial content surpasses 10,000,000,000/milliliter, namely can be used as lactobacillus suspension body bacterial classification inoculation.Wherein said MRS substratum: peptone 10g/L, yeast extract 5 g/L, extractum carnis 10 g/L, glucose 20 g/L, sodium-acetate 5 g/L, dibasic ammonium citrate 2 g/L, tween-80 1 ml/L, sal epsom 0.6 g/L, manganous sulfate 0.05 g/L, dipotassium hydrogen phosphate 2 g/L, agar 20 g/L, calcium carbonate 20 g/L add agar 20 g/L in the solid medium.Wherein said milk-acid bacteria seed substratum: glucose 50 g/L, peptone 15 g/L, extractum carnis 7.5 g/L, sodium-chlor 1 g/L, manganous sulfate 0.01 g/L, sal epsom 0. 2 g/L, sodium-acetate 0. 5 g/L, calcium carbonate 50 g/L, pH6.0.The condition of each medium sterilization is: 0.10-0.15MPa, sterilized 30 minutes for 121 ℃.
2., the preparation of yeast liquid bacterial classification
Take out the culture presevation pipe, draw flat board with the PDA solid medium and recover, 30 degree were cultivated 48 hours.In 50 milliliters of PDA substratum of the single colony inoculation of picking, 30 degree concussions were cultivated 24 hours in incubator under flat board.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 5L, and 30 degree concussions were cultivated 20-28 hour, detected its bacterial concentration, and bacterial content surpasses 500,000,000/milliliter, namely can be used as yeast liquid body bacterial classification inoculation.Wherein said PDA substratum: potato 200g, sucrose 20g, water 1000mL adds agar 20 g/L in the solid medium.Wherein said yeast seed substratum: glucose 10 g/L, yeast extract paste 3 g/L, peptone 5 g/L, ammonium sulfate 5 g/L, sal epsom 0.5 g/L.The condition of each medium sterilization is: 0.10-0.15MPa, sterilized 30 minutes for 121 ℃.
3., the preparation of subtilis liquid spawn
Take out the culture presevation pipe, draw flat board with the LB solid medium and recover, 37 degree were cultivated 48 hours.In 50 milliliters of LB substratum of the single colony inoculation of picking, 37 degree concussions were cultivated 24 hours in incubator under flat board.Seed adopts 5% inoculum size, is seeded in the large triangular flask of 5L, and 37 degree concussions were cultivated 20-28 hour, detected its bacterial concentration, and gemma content surpasses 6,000,000,000/milliliter, namely can be used as the subtilis liquid-spawn inoculation.Wherein said LB substratum: peptone 5g, extractum carnis 3g, sodium-chlor 5g, water 1000mL, pH7.2 adds agar 2% in the solid medium.Wherein said subtilis liquid spawn culture medium: glucose 10g/L, Semen Maydis powder 8 g/L, bean cake powder 25 g/L, sal epsom 0.5 g/L, manganous sulfate 0.5 g/L, SODIUM PHOSPHATE, MONOBASIC 0.5 g/L, Sodium phosphate dibasic 2.3 g/L, pH7.2.The condition of each medium sterilization is: 0.10-0.15MPa, sterilized 30 minutes for 121 ℃.
4., the preparation of photosynthetic bacterium liquid bacterial classification
Take out the culture presevation pipe, draw flat board with photosynthetic bacterium solid culture medium and recover, 35 degree were cultivated 72 hours in illumination box.Intensity of illumination: 1000lex-6000lex.In 50 milliliters of photosynthetic bacterium liquid of the single colony inoculation of picking substratum, 35 degree anaerobism were cultivated 120 hours in illumination box under flat board.Seed adopts 10% inoculum size, is seeded to the large triangular flask of 5L, and 35 degree anaerobism were cultivated 72-96 hour in illumination box, detected its bacterial concentration, and bacterial content surpasses 6,000,000,000/milliliter, namely can be used as the inoculation of solution of photosynthetic bacteria body bacterial classification seed.Wherein said photosynthetic bacterium solid medium: potassium primary phosphate 1g, calcium chloride 0.1g, yellow soda ash 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, agar 20g, pH7.5, water 1000mL.Wherein said photosynthetic bacterium liquid substratum: potassium primary phosphate 1g, calcium chloride 0.1g, yellow soda ash 3g, sodium-acetate 1g, magnesium chloride 0.5g, ammonium chloride 1g, sodium-chlor 1g, pH7.5, water 1000mL.The condition of each medium sterilization is: 0.10-0.15MPa, sterilized 30 minutes for 121 ℃.
5., the preparation of Jingyang streptomycete liquid spawn
Take out the culture presevation pipe, draw flat board with Jingyang streptomycete solid medium and recover, 30 degree were cultivated 72 hours in incubator.In the streptomycete liquid nutrient medium of 50 milliliters of Jingyangs of the single colony inoculation of picking, 30 degree concussions were cultivated 72 hours in incubator on flat board.Seed adopts 10% inoculum size, is seeded in the large triangular flask of 5L, and 30 degree concussions were cultivated 72-96 hour, detected its bacterial concentration, and bacterial content surpasses 100,000,000/milliliter, namely can be used as Jingyang streptomycete liquid spawn seed inoculation.Wherein said Jingyang streptomycete solid medium: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, agar 20g, water 1000ml, pH 7.2-7.4.Wherein said Jingyang streptomycete seed substratum: saltpetre 1g, Zulkovsky starch 20g, dipotassium hydrogen phosphate 0.5g, sal epsom 0.5g, sodium-chlor 0.5g, ferrous sulfate 0.01g, water 1000ml, pH 7.2-7.4.The condition of each medium sterilization is: 0.10-0.15MPa, sterilized 30 minutes for 121 ℃.
Adopt the beneficial effect of technique scheme: this complex microorganism liquid bacterial fertilizer contains photosynthetic bacterium, and photosynthetic bacterium can promote soil material to transform, and improves Soil structure, increases soil fertility, and promotes plant growth.Photosynthetic bacterium is mostly had a nitrogen fixing capacity, can improve the soil nitrogen level, can effectively improve some organic composition, sulfide, ammonia-state nitrogen in the soil by its Metabolic activity, and promotes the conversion of noxious pollutant (such as agricultural chemicals, chemical fertilizer).Can promote simultaneously beneficial microorganism propagation, make the material cycle of common participation soil ecology.In addition, the abundant physiologically active substance that photosynthetic bacterium produces such as proline(Pro), uridylic, cytosine(Cyt), VITAMIN, ubiquinone, carotenoid etc. can both directly be absorbed by plant, help to improve crop alimentary, the activity of activated plant cell, promote root system development, improve photosynthesis and reproductive growth ability.Contain several physiological active substances in the Fermentation by Photosynthetic Bacteria liquid, such as Chlorophyll A, B, carotenoid, multivitamin (especially vitamin B group), various plants hormone (such as IAA, GA3, ABA, ethene, phytokinin), nucleic acid, Whitfield's ointment, multiple amino acids and single-cell protein etc.These physiologically active substances have or promote the accumulation of some specific component in plant root growth or the growth of promotion plant leaf or the promotion fruit or increase crop yield, thereby improve quality of agricultural product.Photosynthetic bacterium can strengthen crop disease-resistant diseases prevention ability.Photosynthetic bacterium contains antibacterium, antiviral material, the virulence of these substance passivation pathogenic agent and inhibition pathogenic growth.The simultaneously activity of photosynthetic bacterium can promote the beneficial microorganism propagation such as actinomycetes, suppresses the pernicious bacteria growths such as filamentous fungus, thereby effectively suppresses some generation of planting disease and spread.Because photosynthetic bacterium has the defending and fighting against diseases effect, can prolong the fruit shelf lives.Photosynthetic bacterium can be improved the plant chlorophyll content, promote the growth of the microorganism species such as vinelandii, root nodule bacterium, actinomycetes in the soil, after tobacco, radish, sweet potato, Soybean and Other Crops spray photosynthetic bacterium, all can improve vinelandii in the soil, root nodule bacterium, actinomycetes and bacterial number, filamentous fungus then reduces, and plays the effect of soil remediation.Photosynthetic bacterium also has and alleviates agricultural chemicals to murder by poisoning and the deteriorating pesticide residue of farm crop.
This complex microorganism liquid bacterial fertilizer contains yeast, yeast utilizes the sugar in the molasses, synthetic amino acid, carbohydrate and other organic substances of secretory product, photosynthetic bacteria that plant root produces to produce fermenting power, synthetic root growth and the fissional active substance of promoting.Yeast can promote other effective microbes (such as milk-acid bacteria, actinomycetes) to breed needed matrix (food) provides important subsistence support.
This complex microorganism liquid bacterial fertilizer contains milk-acid bacteria.It forms lactic acid by the carbohydrate that picked-up photosynthetic bacterium, yeast produce.Lactic acid has very strong sterilizing ability, can the movable and organic rapid corrupt decomposition of establishment harmful microbe.Milk-acid bacteria can be decomposed under normal conditions not labile xylogen and Mierocrystalline cellulose, and organic matter fermentation is decomposed.Milk-acid bacteria can also suppress the pathogenic bacterium propagation that continuous cropping obstacle produces.Pathogenic bacterium are active, and harmful nematode can sharply increase, and plant will be weak, and milk-acid bacteria has suppressed pathogenic bacterium, and harmful nematode just can fade away.
This complex microorganism liquid bacterial fertilizer contains the Jingyang streptomycete, the Jingyang streptomycete is a kind of actinomycetes, the Metabolic activity of Jingyang streptomycete can promote organic substance decomposing, make the nutrient effectuation, simultaneously can discharge antimicrobial substance, thereby effectively prevent the various crop disease and pest, and can produce the phytokinin of stimulating plant growth, it can also promote the formation of soil agreegate, have the formation that promotes soil ulmin and the ability that produces polysaccharide, the mixed clean bacterium effect of Jingyang streptomycete and photosynthetic bacterium is more much bigger than the lethality of Jingyang streptomycete individual combat.The Jingyang streptomycete also can promote vinelandii and VA mycodomatium bacterium propagation.
This complex microorganism liquid bacterial fertilizer contains rich sporiferous subtilis, genus bacillus is the important component part of Plant diseases Biocontrol microorganism, has significant Biocontrol Potential, can produce the contrary gemma of heat-resistant, the production, the formulation that are beneficial to biocontrol fungicide reach survival in environment, surely grow and breeding.At stability, and the aspects such as consistency of chemical pesticide, obviously be better than non-genus bacillus and fungi biocontrol fungicide.It can prevent and treat the plurality of plant diseases such as cotton wilt, tobacco mosaic virus (TMV), take-all, rice blast, leaf blight, cucumber at seedling stage samping off, potato scab, vegetable sprout term dry thread Pyrenomycetes, spinach blight, wheat scab, the red carninomatosis of apple, root rotof flax, ring rot of apple.
This complex microorganism liquid bacterial fertilizer contains a large amount of Chinese prickly ashes, capsicum, and Ginkgo Leaf, China tree, kuh-seng, white plant hopper can be driven away or kill to the vegetable materials such as garlic, aphid, nematode, looper, oriental tobacco budworm, the insects such as tangerine a red-spotted lizard, leek ground maggot, eucleid caterpillar.Destroyed its cell walls in the various probiotics fermention processes effective ingredient is discharged in a large number, greatly strengthened its deinsectization in the probiotics fermention process, the ability of kill harmful bacterium has reduced the utilization of its vegetable material.
This complex microorganism liquid bacterial fertilizer contains abundant calcium, magnesium, trace element, soybean protein, and under multiple bacterium co-fermentation, produce a large amount of organic acids, amino acid and many active substances, organic acid in these elements and the fermented liq, amino acid are complex status or negative and positive particle equilibrium state, namely be in easily by the lytic activity state of plant absorbing, play multi-efficiencies such as replenishing the calcium, mend magnesium, fertilising, rehabilitating soil, volume increase, this also is the advantage of microbial liquid fertilizer, is that other chemical fertilizer and trace element can't replace.
Above-mentioned component cooperate have that probiotics viable bacteria content is high, the vegetable material consumption is few, active high, stability is high, action effect is fast; kill harmful bacterium, controlling plant diseases worm, replenish the calcium, mend the multi-efficiency of magnesium, fertilising; and undergo microbial fermentation its effect is multiplied; many things are once finished; greatly reduce labour intensity; raw material mainly is probiotic bacterium, frequently seen plants fruit and leaf and sugar refinery waste liquid; cost is low; environment is not only formed destruction, and also have very large provide protection.This technology tallies with the national condition and the agricultural development direction, has broad application prospects at agricultural planting.
Below in conjunction with test the field effect (following abbreviation bacterial manure) that the present invention prevents and treats the complex microorganism liquid bacterial fertilizer of plant pest is described.
One, the effect of complex microorganism liquid bacterial fertilizer on tobacco
1. test site: Cotton Varieties by Small Farming Households ground, Long Zhu village, beachhead town, Longhui County, Hunan Province
2. test method: flue-cured tobacco cultivars: middle cigarette 99-10-1, during the flue-cured tobacco transplanting seedling, with 500 times of dilutions of bacterial manure liquid irrigating root once; Sprayed for the first time with 250 times of liquid of bacterial manure in 20 days behind the slow seedling, then sprayed every 20 days continuously for the second time and for the third time (altogether every mu with 4 kilograms in bacterial manure).The check plot when spraying with 250 times of liquid of bacterial manure, with the clear water sprinkling of same consumption at every turn;
3. test-results:
After using the complex microorganism liquid bacterial fertilizer, plant height, stem girth, pitch, leaves number, maximum leaf area are all greater than the contrast that sprays clear water, and explanation can effectively improve the economical character of cigarette strain, promote the healthy and strong growth of cigarette strain.Cigarette strain sickness rate and the occurring degree used significantly reduce, and disease resistance that can the strain of Effective Raise cigarette has good control effect to mosaic disease especially.As can be seen from Table 1, a situation arises according to the Major Diseases of field investigation, and sickness rate and the disease index of balck shank obviously reduce; Bacterial wilt does not find in contrast a small amount of generation is only arranged at treatment zone; Very low for the treatment of zone mosaic disease sickness rate and disease index is 6.3 and 1.2 only, reaches and controls extremely significantly effect;
Table 1 complex microorganism liquid bacterial fertilizer is to the tobacco disease prevention effect
Figure 953976DEST_PATH_IMAGE002
Table 2 complex microorganism liquid bacterial fertilizer is to tobacco production output value the effect of increasing income
Process Output (kg/acre) The output value (unit/mu) Average price (unit/kilogram)
The check plot 198.2 2505.2 12.64
Treatment zone 247.4 3527.9 14.26
Use tobacco premature ripening behind the complex microorganism liquid bacterial fertilizer, improve quality, improve to a great extent output, average price, the output value, the upper medium grade cigarette ratio of tobacco leaf.Can analyze from table 2 and to draw, experiment and demonstration district yield of tobacco, the output value, average price respectively contrast zone exceed 49.2 kg/acres, 1022.7 yuan/mu, 1.62 yuan/kilogram.Deduct 370 yuan of the costs (wherein artificial 310 yuan) of increase, can have a net increase of 652.7 yuan/mu.Using this bacterial fertilizer products can make the tobacco leaf per mu yield increase by 24.8%, tobacco leaf per mu yield value net increase 40.8%.
Two, the test effect of complex microorganism liquid bacterial fertilizer on paddy rice
1. test site: Long Zhu village, beachhead town, Longhui County, Hunan Province peasant household good farmland
2. test method:
⑴. with 100 times of complex microorganism liquid bacterial fertilizer diluent seed soaking 6-8 hour;
⑵. spray once with 250 times of diluents of complex microorganism liquid bacterial fertilizer of 1000 milliliters/mu respectively in seeding stage, tillering phase, boot stage;
3. test-results:
⑴. the impact effect to the paddy rice percentage of germination is best. and can promote seed germination, take root, improve germinating energy and percentage of germination, germinating energy amplification is 7.9～10.75%, and percentage of germination amplification is 8.8～9.5%, and it is fast and neat to germinate, well developed root system, thick white root is many, and adult seedling rate is high;
⑵. per unit area yield is grown, improved to raising rice seedlings and grown in field all positive effect, and especially to improving seedling quality, height survives seedling, and raising thousand seed weight etc. has remarkable effect.Plot experiment is along with using the difference in period, and every mu can be increased production paddy 52-96 kilogram, and amount of increase in production is 8～12%, additional income 104-200 unit, and 60 yuan of deduction costs, mu have a net increase of income 44-140 unit;
⑶. improve the drought resisting of paddy rice, the resistance such as disease-resistant, resistant to lodging, to various soil-borne diseases and bacterial disease, especially the diseases such as rice blast, banded sclerotial blight, damping-off are had stronger inhibition and an effect of prevention.
Three, the effect of complex microorganism liquid bacterial fertilizer on apple
1. test site: Qiansuo Town, Suizhong County, Liaoning Province
2. test method: test is divided into: the check plot is exactly the zone of not using bacterial manure.The test site, the zone of using bacterial manure.The operation of two other fertilizer of Zoned application and other farming is identical, rushes application of water, and contrast also uses identical water.Test site: in spring, 500 times of punchings of bacterial manure dilution are executed or spreaded manuer in holes.In apple flowering period and fruit expanding period with each spraying of 250 times of diluents of bacterial manure once (mu is used 5 kilograms altogether);
3. test-results
After using foliage-spray flowering period, investigation finds that the apple fruit setting rate obviously improves, and compares with contrast, and the test site percentage of fertile fruit improves 8-10 %; After expanding stage uses, compare with contrast, test site apple deformity number obviously reduces, during results, the one-level fruit contrast district that uses bacterial manure has improved 18%, and fruit size is more even, output obviously improves, test site yield increased district output increased 20%; The more important thing is that fruit color is even, the surface is round and smooth, and brightness increases, and pol increases 2-3 degree Beaume, and the shape delicious food is better.The check plot has apple the symptom of bitter pit, elephant skin, water core, casting skin deliquescing to occur, and test site bacterial manure contains calcium, magnesium and the trace element of the needed various chelating attitudes of fruit tree, is easy to fruit tree absorb, and these symptoms do not occur.
Four, complex microorganism liquid bacterial fertilizer test effect on strawberry
1. test site: plantation family, Tao Hong town, Longhui County, Hunan Province
2. test method: at setting date, seedling is planted, with 500 times of dilutions of bacterial manure liquid irrigating root 1 time,
At the strawberry growing period, foliage-spray 1:250 times bacterial manure, 10d(days) 1 time, connect and spray 3 times;
3. test-results:
Found that, the Blooming result, fruit is large and full, and number of results is many, heterocarp is few, mouthfeel is good, and is painted vivid, the single plant yield average out to 126.96g of strawberry (Feng Xiang), contrast (not spraying bacterial manure) improves 28.07%, and use that the soluble solid content of strawberry fruit reaches 10.12% behind the bacterial manure, contrast is relatively high by 13.8%, simultaneously, after using bacterial manure, acid content reduces, and Vitamin C content improves, the local flavor of fruit and quality be improved significantly, and anti-storage, more than 10 days, the strawberry root is stout and strong than the strawberry of the not using bacterial manure storage of Duoing, and Powdery Mildew and the root rot of strawberry have not had yet.Use the output increased 28% in the contrast district, plot of bacterial manure, pol contrast district has increased the 2-3 degree.
Five, the application test of complex microorganism liquid bacterial fertilizer on muskmelon
1, test period: in February, 2011-June
2, test site: plantation family, Cang County, Hebei province booth
3, test method: use 100 times of vacuole kinds of bacterial manure 2 hours; Emerge sprayed for the first time with 250 times of liquid of bacterial manure in rear 10 days, then sprayed every 20 days continuously for the second time to the 4th time (altogether every mu with 4 kilograms in bacterial manure);
4, test-results:
⑴. output increases, quality improvement.Muskmelon per mu yield with bacterial manure reaches 4689 kilograms, contrast volume increase 18.2%, and single melon weighs 1.46 kilograms, and contrast increases 0.214 kilogram; Refractive power pol 16.8, contrast improves 1.62;
⑵. disease is light, and disease resistance is strong.According to investigation on June 25, the disease index 4.5 of oidium, 12.3 of contrast district has reduced by 63.4%.The disease index 6.5 of Powdery Mildew, 10.3 of contrast district has reduced by 34%.There are a large amount of melon aphids at back, check plot later stage blade face, but the test site there is no;
⑶. less investment, high efficiency uses every mu in bacterial manure to increase input 60 yuan, and the recruitment cost increases by 100 yuan, always increases by 160 yuan, can increase about 500 yuan of net return, input-output ratio 1:3.
Six, the complex microorganism liquid bacterial fertilizer is at the effect of tomato
1. test period: in June, 2011-September
2. test site: plantation family, Du Lin township, Cang County, Hebei province
3. test method: before transplanting seedling is stained with 30 minutes kinds of root with 200 times of bacterial manure diluents, after after the field planting 10 days, with 500 times of dilutions of bacterial manure liquid irrigating root 1 time, at the tomato growing period, foliage-spray 1:250 times bacterial manure 20 days 1 time, connects and sprays 3 times;
4. test-results: found that, the Blooming result, fruit is large and full, and number of results is many, and heterocarp is few, mouthfeel is good, painted vivid, the average plant height of tomato contrasts (not spraying bacterial manure) and improves 11%, and yield increased increases 23%, compare photograph, can effectively prevent the tomato fruit blossom-end rot that is caused by calcium deficiency.
Seven, the complex microorganism liquid bacterial fertilizer is to the sick test of Chinese cabbage Perishing prevention
1, test period: 9-2010 October in 2010, spray before at 9 o'clock in the morning or after at 4 o'clock in afternoon;
2, test site: Cangzhou City peasant household vegetable plot;
3, test method: bacterial manure 1:250 is doubly diluted fully, spray the leaf positive and negative.Heart stage spray 1-2 time is wherein wrapped in opening quotation phase, bag heart stage spray 2-3 time.The check plot is with 3000～4000 times of liquid foliage-sprays of agricultural streptomycin of 72%;
4, test effect: spray investigation in rear 2 days, fallen ill stop the morbidity; Investigation in the 4th day, plant restore normal growth.November, the contrast district increased production 10.8% when gathering in the crops.
Eight, the complex microorganism liquid bacterial fertilizer is to Radix Gossypii maize ear rot control test
1, test period: in August, 2010-2010 year September
2, test site: Cangzhou City peasant household cotton field, the annual rotten pathogenetic zone of Radix Gossypii that has in various degree;
3, test method: bacterial manure 1:500 doubly dilutes overcast and rainy rear filling root.Fill with once behind every two weeks, the bacterial manure usage quantity is each 1 liter/mu again;
4, test-results: the check plot sickness rate reaches 20-40%, and executes the not generation disease of our bacterial manure.Reach 45% with the check plot volume increase.
Nine, the complex microorganism liquid bacterial fertilizer is to the control test of radish aphid
1, test period: on February 13rd, 2011, at 4 o'clock in afternoon
2, test site: plantation vegetable plot, family, Longhui County, Hunan Province
3, test method: bacterial manure 1:250 doubly dilutes and sprays;
4, test-results: spray rear second day, green aphid is all dead.Black aphid has not had basically yet, should have been driven away by the smell of bacterial manure.
Ten, the complex microorganism liquid bacterial fertilizer is to the control of ginger root knot nematode
1, test site: plantation vegetable plot, family, Longhui County, Hunan Province
2, test method: in ginger children's phase, bacterial manure 1:250 doubly dilutes and sprays once.In ginger vegetative period, bacterial manure 1:500 doubly dilutes rear filling root, and utilizes simultaneously bacterial manure 1:250 doubly to dilute to spray once;
3, test-results: the ginger of general planting, rot, root knot nematode are serious, and be disease-free with the ginger of bacterial manure.
Embodiment
Substantive distinguishing features of the present invention can be embodied from the following examples, but these embodiment only as an illustration, rather than limits the invention.
Embodiment 1
The raw material for preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 30 parts of mashed garlics, 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, greater than 10 parts of 6,000,000,000/milliliter photosynthetic bacteriums, greater than 10 parts of 500,000,000/ml yeast bacterium, greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, greater than 10 parts of 6,000,000,000/milliliter subtilises, 800 parts in water.
The preparation method of the complex microorganism liquid bacterial fertilizer of this control plant pest comprises the steps:
⑴, successively adding in fermentor tank: 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 800 parts in water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add 30 parts of mashed garlics, 6,000,000,000/10 parts of photosynthetic bacteriums of milliliter, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 10 parts of 6,000,000,000/milliliter subtilises;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.
Embodiment 2
The raw material for preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 20 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 20 parts of mashed garlics, 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, greater than 15 parts of 6,000,000,000/milliliter photosynthetic bacteriums, greater than 5 parts of 500,000,000/ml yeast bacterium, greater than 5 parts of 10,000,000,000/milliliter plant lactobacillus, greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, greater than 10 parts of 6,000,000,000/milliliter subtilises, 800 parts in water.
The preparation method of the complex microorganism liquid bacterial fertilizer of this control plant pest comprises the steps:
⑴, successively adding in fermentor tank: 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, 20 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 10 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 810 parts in water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add 20 parts of mashed garlics, 6,000,000,000/15 parts of photosynthetic bacteriums of milliliter, 5 parts of 500,000,000/ml yeast bacterium, 5 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 10 parts of 6,000,000,000/milliliter subtilises;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.
Embodiment 3
The raw material for preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 30 parts of mashed garlics, 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, greater than 15 parts of 6,000,000,000/milliliter photosynthetic bacteriums, greater than 10 parts of 500,000,000/ml yeast bacterium, greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, greater than 15 parts of 6,000,000,000/milliliter subtilises, 800 parts in water.
The preparation method of the complex microorganism liquid bacterial fertilizer of this control plant pest comprises the steps:
⑴, successively adding in fermentor tank: 50 parts in molasses, 5 parts of analysis for soybean powder, 10 parts in calcium sulfate, 10 parts in magnesium chloride, 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 5 parts of ginkgo leaf powders, 5 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 800 parts in water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add 30 parts of mashed garlics, 6,000,000,000/15 parts of photosynthetic bacteriums of milliliter, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 15 parts of 6,000,000,000/milliliter subtilises;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.
Embodiment 4
The raw material for preparing the complex microorganism liquid bacterial fertilizer of this control plant pest is by weight: 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 20 parts of mashed garlics, 50 parts in molasses, 5 parts of analysis for soybean powder, 15 parts in calcium sulfate, 10 parts in magnesium chloride, greater than 10 parts of 6,000,000,000/milliliter photosynthetic bacteriums, greater than 10 parts of 500,000,000/ml yeast bacterium, greater than 10 parts of 10,000,000,000/milliliter plant lactobacillus, greater than 10 parts of 100,000,000/milliliter Jingyang streptomycetes, greater than 15 parts of 6,000,000,000/milliliter subtilises, 800 parts in water.
The preparation method of the complex microorganism liquid bacterial fertilizer of this control plant pest comprises the steps:
⑴, successively adding in fermentor tank: 50 parts in molasses, 5 parts of analysis for soybean powder, 15 parts in calcium sulfate, 10 parts in magnesium chloride, 10 parts of zanthoxylum powders, 10 parts of red chilly powders, 10 parts of ginkgo leaf powders, 10 parts in China tree powder, 5 parts in kuh-seng powder, 5 parts of salt, 800 parts in water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add 20 parts of mashed garlics, 6,000,000,000/10 parts of photosynthetic bacteriums of milliliter, 10 parts of 500,000,000/ml yeast bacterium, 10 parts of 10,000,000,000/milliliter plant lactobacillus, 10 parts of 100,000,000/milliliter Jingyang streptomycetes, 15 parts of 6,000,000,000/milliliter subtilises;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.

Claims (7)

1. a preparation method who prevents and treats the complex microorganism liquid bacterial fertilizer of plant pest is characterized in that the raw material for preparing this bacterial manure is by weight: zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 5-15 part, China tree powder 5-15 part, kuh-seng powder 5-15 part, molasses 40-80 part, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, salt 5-10 part, mashed garlic 20-40 part, greater than 6,000,000,000/milliliter photosynthetic bacterium bacterial classification 5-15 part, greater than 500,000,000/ml yeast bacterium bacterial classification 5-15 part, greater than 10,000,000,000/milliliter plant lactobacillus bacterial classification 5-15 part, greater than 100,000,000/milliliter Jingyang streptomyces species 5-15 part, greater than 6,000,000,000/milliliter Bacillus subtilis strain 5-15 part, 800 parts in water.
2. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1 is characterized in that, comprises the steps:
⑴, in fermentor tank, add successively: 800 parts of molasses 40-80 parts, analysis for soybean powder 4-8 part, calcium sulfate 5-15 part, magnesium chloride 5-15 part, zanthoxylum powder 10-20 part, red chilly powder 10-20 part, ginkgo leaf powder 5-15 part, China tree powder 5-15 part, kuh-seng powder 5-15 part, salt 5-10 part, water, heated and boiled 30 minutes is cooled to 40 degree;
⑵, the cooling fluid of producing at step ⑴ add mashed garlic 20-40 part, greater than 6,000,000,000/milliliter photosynthetic bacterium bacterial classification 5-15 part, greater than 500,000,000/ml yeast bacterium bacterial classification 5-15 part, greater than 10,000,000,000/milliliter lactic acid bacteria culturers 5-15 part, greater than 100,000,000/milliliter Jingyang streptomyces species 5-15 part, greater than 6,000,000,000/milliliter Bacillus subtilis strain 5-15 part;
⑶, 30-35 degree were cultivated 120-150 hour, stirred 30 minutes in per 10 hours;
, again the static cultivation of 30-35 degree 120-150 hour, detect the viable bacteria total content greater than 4,000,000,000/milliliter;
⑸, emit bacterium liquid with four layers of filtered through gauze, packaging final prod.
3. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1 is characterized in that, mashed garlic be will cooling boiling water with etc. after the garlic of weight mixes, pulling an oar makes.
4. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1, it is characterized in that zanthoxylum powder, red chilly powder, ginkgo leaf powder, China tree powder, kuh-seng powder are that the Chinese prickly ash that will buy from the market, capsicum, Ginkgo Leaf, China tree, kuh-seng etc. were pulverized 60 orders-100 order gained.
5. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1 is characterized in that, the photosynthetic bacterium bacterial classification is Rhodopseudomonas palustris, Rhodopseudomonas spheroides, one or more in the rhodospirillum such as capsula Rhodopseudomonas.
6. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1 is characterized in that, barms is yeast saccharomyces cerevisiae, Candida utilis, one or more in the candida tropicalis.
7. the preparation method of the complex microorganism liquid bacterial fertilizer of control plant pest according to claim 1 is characterized in that, lactobacillus inoculation is plant lactobacillus, Lactobacterium acidophilum, one or more in the lactobacterium casei.
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