CN107778109A - A kind of agricultural complex micro organism fungicide and preparation method thereof - Google Patents
A kind of agricultural complex micro organism fungicide and preparation method thereof Download PDFInfo
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- CN107778109A CN107778109A CN201711076317.3A CN201711076317A CN107778109A CN 107778109 A CN107778109 A CN 107778109A CN 201711076317 A CN201711076317 A CN 201711076317A CN 107778109 A CN107778109 A CN 107778109A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
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Abstract
The present invention relates to a kind of agricultural complex micro organism fungicide, and it includes the component of following parts by weight：Sophora alopecuroide Radix Sophorae Flavescentis extractive liquid 100mL, the 1mL of persimmon leaf flavone crude extract 0.1, the 20g of inorganic nutrients composition 10 and the 2g of bacteria agent 0.5.The invention further relates to the preparation method of above-mentioned microbial inoculum.The present invention is the agricultural complex micro organism fungicide for having growth promoting and disease-proof functions, and botanical pesticide and bacteria agent are carried out into efficient combination, is applied as punching or foliar fertilizer uses in vegetable cultivation, preventive effect effect is good, growth promoting is obvious, safety and environmental protection.
Description
Technical field
The invention belongs to agricultural microbial agent, and in particular to it is a kind of with diseases prevention, growth-promoting function microbial bacterial agent and
Its preparation method.
Background technology
A large amount of apply of chemical pesticide and chemical fertilizer causes serious environmental pollution and agricultural planting obstacle, including soil
Earth organic matter reduces, the soil salinization aggravates, pest and disease damage resistance increases, agricultural product medicament residue is exceeded etc., therefore, using nature
Nutriment and the microbial resources with disease prevention growth-promoting carry out the exploitation of complex micro organism fungicide to reducing crop growth mistake
Chemical pesticide and chemical fertilizer dosage have the function that important in journey.Therefore, the present invention utilizes Sophora alopecuroide, kuh-seng, persimmon leaf etc.
Natural extract is compounded with microorganism, to form the fertilizer for vegetables diseases prevention, the plant type of growth-promoting function or microbiology class
Material exploitation, to reducing the fertile survival dose of chemistry, raising quality of agricultural product has the function that important.
The content of the invention
It is an object of the invention to provide a kind of agricultural complex micro organism fungicide with growth promoting and disease-proof functions, by plant source
Agricultural chemicals carries out efficient combination with bacteria agent, is applied as punching or foliar fertilizer uses in vegetable cultivation, and preventive effect effect is good, promotees
It is long obvious, safety and environmental protection.
The present invention adopts the following technical scheme that：
A kind of agricultural complex micro organism fungicide, it includes the component of following parts by weight：Sophora alopecuroide-Radix Sophorae Flavescentis extractive liquid 100mL, persimmon leaf
Flavones crude extract 0.1-1mL, inorganic nutrients composition 10-20g and bacteria agent 0.5-2g.
Further, the inorganic nutrients composition includes the component of following parts by weight：The urea of 20-30 parts, 30-50 parts
Dipotassium hydrogen phosphate and the potassium nitrate of 20-50 parts.
Further, the bacteria agent includes the component of following parts by weight：The bacillus megaterium microbial inoculum of 30-50 parts and
The bacillus amyloliquefaciens microbial inoculum of 50-70 parts.
Further, the Sophora alopecuroide-Radix Sophorae Flavescentis extractive liquid is prepared via a method which：Weigh Sophora alopecuroide 10-30g and kuh-seng
10-20g, soaked 1 day, boiled 2 hours with 100mL clear water, 2 layers of filtered through gauze remove residue, collect liquid and supply 100mL.
Further, the Sophora alopecuroide-Radix Sophorae Flavescentis extractive liquid is prepared via a method which：Weigh Sophora alopecuroide 20g and kuh-seng
15g, soaked 1 day, boiled 2 hours with 100mL clear water, 2 layers of filtered through gauze remove residue, collect liquid and supply 100mL.
Further, it includes the component of following parts by weight：Sophora alopecuroide-Radix Sophorae Flavescentis extractive liquid 100mL, persimmon leaf flavone crude extract
0.5mL, inorganic nutrients composition 15g and bacteria agent 1g.
Further, the inorganic nutrients composition includes the component of following parts by weight：25 parts of urea, 40 parts of phosphoric acid hydrogen
Dipotassium and 35 parts of potassium nitrate.
Further, the bacteria agent includes the component of following parts by weight：40 parts of bacillus megaterium microbial inoculum and 60
The bacillus amyloliquefaciens microbial inoculum of part.
Further, the viable count in the bacillus megaterium microbial inoculum and bacillus amyloliquefaciens microbial inoculum be 10,000,000,000/
More than gram.
Further, the bacillus megaterium microbial inoculum obtains for commercially available purchase.The bacillus amyloliquefaciens microbial inoculum is
Bacillus amyloliquefaciens are used as the spray-dried gained of adsorbent after liquid fermentation, using calcium carbonate.Calcium carbonate absorption solution starch
The specific method being spray-dried after bacillus is known technology, and the dosage of calcium carbonate solves starch gemma bar with effective absorption
Bacterium bacterium solution is suitable.The bacillus amyloliquefaciens are the bacillus amyloliquefaciens that deposit number is CGMCC No. 12190
（Bacillus amyloliquefaciens）.
A kind of preparation method of above-mentioned agricultural complex micro organism fungicide, it comprises the following steps：
1）Sophora alopecuroide and kuh-seng are weighed respectively, is soaked 1 day with 100mL clear water, are boiled 2 hours, 2 layers of filtered through gauze remove residue,
Collect liquid and supply 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, carry out the extraction of persimmon leaf flavone, crude extract is obtained, is extract II；
3）Draw persimmon leaf flavone crude extract to add in 100mL extracts I, be vegetable-derived components III；
4）Urea, dipotassium hydrogen phosphate and potassium nitrate are weighed by weight, is well mixed, is added to vegetable-derived components III, and composition is supported
Divide IV；
5）Bacillus megaterium and bacillus amyloliquefaciens are weighed by weight, is well mixed, and are added in the nutrient IV,
It is well mixed, produce.
Step 2）Described in obtain persimmon leaf flavone crude extract（That is extract II）Method be prior art, refer to disclosure
Number for CN1546014A Chinese patent in related content.
The beneficial effects of the present invention are：The present invention using with growth-promoting, the Sophora alopecuroide of prophylaxis effect, kuh-seng, persimmon leaf for plant
Thing material, using bacillus amyloliquefaciens, bacillus megaterium as functional microorganism, add wherein nitrogen, phosphorus, the nutrition such as potassium into
Point, formed with growth promoting, diseases prevention, the complex micro organism fungicide for improving fertilizer utilization efficiency.
The material that plant and microbiology class composition used in the present invention interact for environmental sound and plant growth,
Contain multiple proteins, carbohydrate, organic acid, flavonoids, pigment and alkaloid wherein in Sophora alopecuroide, these materials can promote to plant
Thing growth, the disease resistance for improving systemic resistance of plant, increasing plant；Kuh-seng is legume, containing species alkaloid in the majority and
Glucoside, have the function that to improve plant autoimmunity, promote plant growth, reduce disease.Contain a variety of Huangs in persimmon leaf
Letones, have the function that significantly to promote plant growth, improve plant autoimmunity.Bacillus megaterium is a kind of tool
Have phosphorus decomposing, potassium decomposing, a microbial strains of growth promoting effect, Field information can play regulation soil microenvironment, improved soil,
Improve the effect of utilization rate of fertilizer.Bacillus amyloliquefaciens are a kind of wide variety of growth promoting and disease-resistant microbial strains.On
State that raw material is pollution-free, has no side effect, be the green agricultural material product needed for current agricultural production.
The invention provides a kind of complex microbial inoculum with growth-promoting prophylaxis effect, applicant is following plant growth
Rule is needed on the basis of fertilizer, by plant source disease prevention growth-promoting material and bacteria agent compounding use, the plant source after science compounds
Nutritional ingredient has the function that to promote probiotic microorganisms growth and breeding, so as to more effectively improve the fertilizer efficiency of the microbial bacterial agent
And drug effect.
Embodiment
For a better understanding of the present invention, present disclosure, but this hair are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples.In the following description, give a large amount of concrete details so as to
More thorough understanding of the invention is provided.It will be apparent, however, to one skilled in the art that the present invention can be with
It is carried out without one or more of these details.
Embodiment 1
A kind of complex micro organism fungicide, it is prepared by the raw material of following parts by weight：10 parts of Sophora alopecuroide, 10 parts of kuh-seng, persimmon
0.5 part of leaf flavones crude extract, 10 parts of inorganic nutrients composition, 0.5 part of bacteria agent.
The inorganic nutrients composition includes urea, 30% dipotassium hydrogen phosphate and 50% potassium nitrate that percentage by weight is 20%.
The bacteria agent is by bacillus megaterium microbial inoculum of the percentage by weight for 30% and 70% bacillus amyloliquefaciens bacterium
Agent forms.Bacillus megaterium viable count be more than or equal to 10,000,000,000/gram, bacillus amyloliquefaciens be more than or equal to 10,000,000,000/gram.
Its preparation method, comprise the following steps：
1）Sophora alopecuroide 10g, the kuh-seng 10g of parts by weight are weighed respectively, are soaked 1 day, boiled 2 hours, 2 layers of gauze with 100mL clear water
Residue is filtered to remove, liquid is collected and supplies 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, crude extract is obtained, is extract II；
3）The persimmon leaf flavone crude extract for drawing 0.5mL is added in 100mL extracts I, is vegetable-derived components III；
4）Weighing urea, dipotassium hydrogen phosphate, potassium nitrate by weight percentage makes, the percentage composition for making three kinds of fertilizer is respectively 20%,
30%th, 50%, and be well mixed, weigh 10g and be added to vegetable-derived components III, form nutrient IV；
5）Viable count be more than or equal to 10,000,000,000/gram bacillus megaterium microbial inoculum and bacillus amyloliquefaciens microbial inoculum, respectively weigh
0.25g is added in nutrient IV, is mixed and is produced purpose complex micro organism fungicide.
Embodiment 2
A kind of complex micro organism fungicide, it is prepared by the raw material of following parts by weight：20 parts of Sophora alopecuroide, 20 parts of kuh-seng, persimmon leaf are yellow
0.1 part of ketone crude extract, 15 parts of inorganic nutrients composition, 1 part of bacteria agent.
The inorganic nutrients composition includes urea, 40% dipotassium hydrogen phosphate and 30% potassium nitrate that percentage by weight is 30%.
The bacteria agent is by bacillus megaterium microbial inoculum of the percentage by weight for 50% and 50% bacillus amyloliquefaciens bacterium
Agent forms.Bacillus megaterium viable count be more than or equal to 10,000,000,000/gram, bacillus amyloliquefaciens be more than or equal to 10,000,000,000/gram.
Its preparation method, comprise the following steps：
1）Sophora alopecuroide 20g, the kuh-seng 20g of parts by weight are weighed respectively, are soaked 1 day, boiled 2 hours, 2 layers of gauze with 100mL clear water
Residue is filtered to remove, liquid is collected and supplies 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, crude extract is obtained, is extract II；
3）The persimmon leaf flavone crude extract for drawing 0.1mL is added in 100mL extracts I, is vegetable-derived components III；
4）Weighing urea, dipotassium hydrogen phosphate, potassium nitrate by weight percentage makes, the percentage composition for making three kinds of fertilizer is respectively 30%,
40%th, 30%, and be well mixed, weigh 15g and be added to vegetable-derived components III, form nutrient IV；
5）Viable count be more than or equal to 10,000,000,000/gram bacillus megaterium microbial inoculum and bacillus amyloliquefaciens microbial inoculum, respectively weigh 0.5g
It is added in nutrient IV, mixes and produce purpose complex micro organism fungicide.
Embodiment 3
A kind of complex micro organism fungicide, it is prepared by the raw material of following parts by weight：30 parts of Sophora alopecuroide, 20 parts of kuh-seng, persimmon leaf
0.8 part of flavones crude extract, 18 parts of inorganic nutrients composition, 1.2 parts of bacteria agent.
The inorganic nutrients composition includes urea, 35% dipotassium hydrogen phosphate and 40% potassium nitrate that percentage by weight is 25%.
The bacteria agent is by bacillus megaterium microbial inoculum of the percentage by weight for 50% and 50% bacillus amyloliquefaciens bacterium
Agent forms.Bacillus megaterium viable count be more than or equal to 10,000,000,000/gram, bacillus amyloliquefaciens be more than or equal to 10,000,000,000/gram.
Its preparation method, comprise the following steps：
1）Sophora alopecuroide 30g, the kuh-seng 20g of parts by weight are weighed respectively, are soaked 1 day, boiled 2 hours, 2 layers of gauze with 100mL clear water
Residue is filtered to remove, liquid is collected and supplies 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, crude extract is obtained, is extract II；
3）The persimmon leaf flavone crude extract for drawing 0.8mL is added in 100mL extracts I, is vegetable-derived components III；
4）Weighing urea, dipotassium hydrogen phosphate, potassium nitrate by weight percentage makes, the percentage composition for making three kinds of fertilizer is respectively 25%,
35%th, 40%, and be well mixed, weigh 18g and be added to vegetable-derived components III, form nutrient IV；
5）Viable count be more than or equal to 10,000,000,000/gram bacillus megaterium microbial inoculum and bacillus amyloliquefaciens microbial inoculum, respectively weigh 0.6g
It is added in nutrient IV, mixes and produce purpose complex micro organism fungicide.
Embodiment 4
A kind of complex micro organism fungicide, it is prepared by the raw material of following parts by weight：18 parts of Sophora alopecuroide, 18 parts of kuh-seng, persimmon leaf are yellow
1 part of ketone crude extract, 20 parts of inorganic nutrients composition, 2 parts of bacteria agent.
The inorganic nutrients composition includes the urea, 50% dipotassium hydrogen phosphate, 20% potassium nitrate group that percentage by weight is 30%
Into.
The bacteria agent is by bacillus megaterium microbial inoculum of the percentage by weight for 45% and 55% bacillus amyloliquefaciens bacterium
Agent forms.Bacillus megaterium viable count be more than or equal to 10,000,000,000/gram, bacillus amyloliquefaciens be more than or equal to 10,000,000,000/gram.
Its preparation method, comprise the following steps：
1）Sophora alopecuroide 18g, the kuh-seng 18g of parts by weight are weighed respectively, are soaked 1 day, boiled 2 hours, 2 layers of gauze with 100mL clear water
Residue is filtered to remove, liquid is collected and supplies 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, crude extract is obtained, is extract II；
3）The persimmon leaf flavone crude extract for drawing 1mL is added in 100mL extracts I, is vegetable-derived components III；
4）Weighing urea, dipotassium hydrogen phosphate, potassium nitrate by weight percentage makes, the percentage composition for making three kinds of fertilizer is respectively 30%,
50%th, 20%, and be well mixed, weigh 20g and be added to vegetable-derived components III, form nutrient IV；
5）Viable count be more than or equal to 10,000,000,000/gram bacillus megaterium microbial inoculum and bacillus amyloliquefaciens, respectively weigh 1.0g addition
Into nutrient IV, mix and produce purpose complex micro organism fungicide.
Effect example
The complex micro organism fungicide that 1 ~ embodiment of Application Example 4 is prepared on tomato, is combined as with the plant source in embodiment 1
Control 1, using microbial source part in embodiment 1 as control 2, using inorganic nutrients composition in embodiment 1 as control 3.The fertilizer is
Root punching is applied, 500 times of extension rate, and continuous administration is twice, administered twice to be spaced 10 days after tomato field planting survives, to be not added with
The processing of fertilizer is blank control.Tomato growth stage statistics tomato yield, malformed fruit number, tomato wilt incidence of disease etc..Knot
Fruit is shown in Table 1.
The different agricultural complex micro organism fungicides of table 1 compare
。
Increase tomato yield, drop are respectively provided with compared with other controls by embodiment 1, embodiment 2 it can be seen from the result of table 1
Low malformed fruit number, reduce the effect of the droop incidence of disease.The vegetable-derived components of embodiment 1 and the complex micro organism fungicide, micro- life
Material resource composition is compared with inorganic nutrients composition, is achieved good volume increase, diseases prevention and the effect for reducing malformed fruit quantity, is shown
Three kinds of compositions have synergistic function, and the effect of vegetable-derived components and microbial source composition in the fertilizer plays main effect and made
With.
Embodiment described above is only that the preferred embodiment of the present invention is described, but is not limited to this, this
The technical staff in field is easy to understand according to above-described embodiment the spirit of the present invention, and makes different amplification and change, but
Without departing from the spirit of the present invention, all within protection scope of the present invention.
Claims (8)
1. a kind of agricultural complex micro organism fungicide, it is characterised in that it includes the component of following parts by weight：Sophora alopecuroide-kuh-seng carries
Take liquid 100mL, persimmon leaf flavone crude extract 0.1-1mL, inorganic nutrients composition 10-20g and bacteria agent 0.5-2g；
The inorganic nutrients composition includes the component of following parts by weight：The urea of 20-30 parts, the dipotassium hydrogen phosphate of 30-50 parts with
And the potassium nitrate of 20-50 parts；
The bacteria agent includes the component of following parts by weight：The bacillus megaterium microbial inoculum of 30-50 parts and the Xie Dian of 50-70 parts
Afnyloliquefaciens microbial inoculum.
2. a kind of agricultural complex micro organism fungicide according to claim 1, it is characterised in that the Sophora alopecuroide-kuh-seng carries
Liquid is taken to be prepared via a method which：Sophora alopecuroide 10-30g and kuh-seng 10-20g are weighed, is soaked 1 day with 100mL clear water, it is small to boil 2
When, 2 layers of filtered through gauze remove residue, collect liquid and supply 100mL.
3. a kind of agricultural complex micro organism fungicide according to claim 2, it is characterised in that the Sophora alopecuroide-kuh-seng carries
Liquid is taken to be prepared via a method which：Sophora alopecuroide 20g and kuh-seng 15g are weighed, is soaked 1 day, boiled 2 hours, 2 layers with 100mL clear water
Filtered through gauze removes residue, collects liquid and supplies 100mL.
4. a kind of agricultural complex micro organism fungicide according to claim 3, it is characterised in that it includes following parts by weight
Component：Sophora alopecuroide-Radix Sophorae Flavescentis extractive liquid 100mL, persimmon leaf flavone crude extract 0.5mL, inorganic nutrients composition 15g and bacteria agent 1g.
5. a kind of agricultural complex micro organism fungicide according to claim 4, it is characterised in that the inorganic nutrients are into subpackage
Include the component of following parts by weight：25 parts of urea, 40 parts of dipotassium hydrogen phosphate and 35 parts of potassium nitrate.
6. a kind of agricultural complex micro organism fungicide according to claim 5, it is characterised in that the bacteria agent is included such as
The component of lower parts by weight：40 parts of bacillus megaterium microbial inoculum and 60 parts of bacillus amyloliquefaciens microbial inoculum.
A kind of 7. agricultural complex micro organism fungicide according to claim 1, it is characterised in that the bacillus megaterium bacterium
The viable count of agent and bacillus amyloliquefaciens microbial inoculum is 10,000,000,000/gram more than.
A kind of 8. preparation method according to any one of the claim 1 ~ 7 agricultural complex micro organism fungicide, it is characterised in that its
Comprise the following steps：
1）Sophora alopecuroide and kuh-seng are weighed respectively, is soaked 1 day with 100mL clear water, are boiled 2 hours, 2 layers of filtered through gauze remove residue,
Collect liquid and supply 100mL, obtain extract I；
2）Mountain area persimmon fallen leaves are collected, carry out the extraction of persimmon leaf flavone, crude extract is obtained, is extract II；
3）Draw persimmon leaf flavone crude extract II to add in 100mL extracts I, be vegetable-derived components III；
4）Urea, dipotassium hydrogen phosphate and potassium nitrate are weighed by weight, is well mixed, is added to vegetable-derived components III, and composition is supported
Divide IV；
5）Bacillus megaterium microbial inoculum and bacillus amyloliquefaciens microbial inoculum are weighed by weight, are well mixed, being added to will be described
In nutrient IV, it is well mixed, produces.
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| CN114853552A (en) * | 2022-06-07 | 2022-08-05 | 新疆绿洲大洋生物科技有限公司 | Saline-alkali soil conditioner and preparation method and application thereof |
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Application publication date: 20180309 |