CN105053044A - Production method of microbial insectproof liquid - Google Patents
Production method of microbial insectproof liquid Download PDFInfo
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- CN105053044A CN105053044A CN201510454632.XA CN201510454632A CN105053044A CN 105053044 A CN105053044 A CN 105053044A CN 201510454632 A CN201510454632 A CN 201510454632A CN 105053044 A CN105053044 A CN 105053044A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a production method of a microbial insectproof liquid. The production method comprises the following steps: inoculating lactobacillus acidophilus into an anaerobic medium, inoculating candida utilis into an aerobic medium, inoculating rhodopseudomonas palustris into a light irradiation medium; when the cell concentration of each bacterium seed solution reaches 106/mL, respectively inoculating the bacteria into a mixed medium, mixing, then carrying out anaerobic fermentation, stopping fermentation when pH value reaches a certain value; mixing capsaicin, citronellal, and lavender essential oil according to a certain ratio, then adding the mixture into the fermentation broth, stirring so as to obtain the finished product. According to the provided production method, three beneficial strains are chosen and compounded reasonably, the selected bacteria are all beneficial and environment-friendly; and the bacteria are worthy for promotion and application. The medium ratio is scientifically compounded, the fermentation technology is reasonable, and different fermentation conditions are provided according to the different physiological-ecological characters of different bacteria. The produced microbial insectproof liquid can kill and dispel insects and kill bacteria.
Description
Technical field
The present invention relates to a kind of production method of microbial pest control liquid.
Background technology
In agricultural production, agricultural chemicals is the indispensable means of production, and the development of agricultural chemicals is to safety, efficient, ecological, environmentally friendly future development.In recent years, along with emergence and the fast development of the ecological agriculture, microbial pesticide is used widely.The microbial pesticide of extensive use is on the market mainly based on bacillus now, comprise the different kind organism agricultural chemicals produced for strain fermentation with the bacterial strain such as bacillus thuringiensis, Bacillus sphaericus, these biopesticides have good desinsection, antibacterial function, but the subject matter existed is: working condition requirement is higher, production cost is higher, it is slow to take effect, the drawbacks such as physical and chemical index and active constituent content instability.These factors drastically influence applying of biopesticide, also become the bottleneck that ecological agriculture fast development not can bypass simultaneously.
Summary of the invention
The object of the invention is just to provide the production method of the microbial pest control liquid that a kind of production technology is reasonable, properties of product are stable, and the microbial pest control liquid that the method is produced, to eco-friendly, safety, has the function of expelling parasite, desinsection, sterilization.
The production method of microbial pest control liquid of the present invention, comprises and gives step:
1, lactobacillus acidophilus is accessed row Anaerobic culturel in anaerobic culture medium, the percentage by weight of Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Cultivated by oxygen of acting charitably in candida utili bacterium access aerobic culture medium, the percentage by weight of aerobic culture medium raw material is, water: molasses: urea: yeast extract=60%:35%:4.5%:0.5%; Rhodopseudomonas palustris is accessed illumination cultivation base row illumination cultivation, illuminance is 3000-4000LX, the percentage by weight of illumination cultivation based raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract: potassium dihydrogen phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
2, when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with in the ratio access mixed culture medium of 1%, the percentage by weight of Mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%, anaerobic fermentation 19-22 days in fermenter is loaded after mixing, stop fermentation to pH value 3.2-3.8, filter, obtain zymotic fluid for subsequent use;
3, capsaicine, citronellal, Lavender are pressed the part by weight mixing of 2:1:1, then press mixture: the part by weight of zymotic fluid=5%:95%, is added into mixture in zymotic fluid and stirs, finished product.
The production method of microbial pest control liquid of the present invention, screened three kinds of safety, meet national relevant laws and regulations, the useful bacterial strain of metabolite tool expelling parasite usefulness, according to the biological property of each bacterial strain, reasonably combined, be combined into fermentation compound bacteria bacterial classification, bacterial classification used is beneficial bacterium, belongs to environmentally friendly mushroom, under the new situation flourish in the ecological agriculture, there is wide popularizing application prospect; According to the nutritional need of bacterial classification, it is medium component that optimum choice can meet nutritional need raw material cheap and easy to get again, and Medium Proportion is with strong points; Zymotechnique is reasonable, according to the physiological ecological habit of different strain, provides the cultivation and fermentation condition being suitable for different strain.
The microbial pest control liquid that the present invention produces, has following functions:
1, expelling parasite: there is repellent performance to most insects; The action principle of expelling parasite is that said preparation has certain excitant, causes most insect to be shied away;
2, desinsection: have interior absorption killing efficiency to Lepidoptera, Hymenoptera sector of breakdown insect; The mechanism of insecticidal efficacy is the digestive function that microbial metabolic products in said preparation and Extracts from Plant Recourses can affect insect, causes its physiologic derangement and dead;
3, sterilization: part mould, fungi tool are suppressed and killing effect; Sterilization mechanism is capsaicine, citronellal, Lavender etc. all have function that is antibacterial and sterilizing.
Embodiment
A production method for microbial pest control liquid, comprises and gives step:
1, lactobacillus acidophilus is accessed row Anaerobic culturel in anaerobic culture medium, the percentage by weight of Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Cultivated by oxygen of acting charitably in candida utili bacterium access aerobic culture medium, the percentage by weight of aerobic culture medium raw material is, water: molasses: urea: yeast extract=60%:35%:4.5%:0.5%; Rhodopseudomonas palustris is accessed illumination cultivation base row illumination cultivation, illuminance is 3000-4000LX, the percentage by weight of illumination cultivation based raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract: potassium dihydrogen phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
2, when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with in the ratio access mixed culture medium of 1%, the percentage by weight of Mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%, anaerobic fermentation 19-22 days in fermenter is loaded after mixing, stop fermentation to pH value 3.2-3.8, filter, obtain zymotic fluid for subsequent use;
3, capsaicine, citronellal, Lavender are pressed the part by weight mixing of 2:1:1, then press mixture: the part by weight of zymotic fluid=5%:95%, is added into mixture in zymotic fluid and stirs, finished product.
Claims (1)
1. a production method for microbial pest control liquid, comprises and gives step:
(1), by lactobacillus acidophilus access row Anaerobic culturel in anaerobic culture medium, the percentage by weight of Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Cultivated by oxygen of acting charitably in candida utili bacterium access aerobic culture medium, the percentage by weight of aerobic culture medium raw material is, water: molasses: urea: yeast extract=60%:35%:4.5%:0.5%; Rhodopseudomonas palustris is accessed illumination cultivation base row illumination cultivation, illuminance is 3000-4000LX, the percentage by weight of illumination cultivation based raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract: potassium dihydrogen phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
(2), when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with in the ratio access mixed culture medium of 1%, the percentage by weight of Mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%, anaerobic fermentation 19-22 days in fermenter is loaded after mixing, stop fermentation to pH value 3.2-3.8, filter, obtain zymotic fluid for subsequent use;
(3), by capsaicine, citronellal, Lavender press the part by weight mixing of 2:1:1, then press mixture: the part by weight of zymotic fluid=5%:95%, is added into mixture in zymotic fluid and stirs, finished product.
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| CN201510454632.XA CN105053044A (en) | 2015-07-30 | 2015-07-30 | Production method of microbial insectproof liquid |
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| CN201510454632.XA CN105053044A (en) | 2015-07-30 | 2015-07-30 | Production method of microbial insectproof liquid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111149814A (en) * | 2020-01-14 | 2020-05-15 | 河南农贝得农业科技有限公司 | Agricultural microbial agent with insect expelling effect and preparation method thereof |
| CN111543443A (en) * | 2020-06-12 | 2020-08-18 | 江苏益睦生物科技有限公司 | Biological insect-resist agent and preparation method and application thereof |
| CN113693084A (en) * | 2021-08-27 | 2021-11-26 | 烟台凯多海洋生物研究院有限公司 | Fig leaf-derived insect repellent and preparation and application thereof |
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| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN102910988A (en) * | 2012-11-18 | 2013-02-06 | 蒋常德 | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer |
| CN103911312A (en) * | 2013-09-13 | 2014-07-09 | 领绿生物镇江有限公司 | Microbial preparation with efficacy of purifying water, fertilizing water and caring health and preparation method thereof |
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- 2015-07-30 CN CN201510454632.XA patent/CN105053044A/en active Pending
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| CN101270335A (en) * | 2007-03-21 | 2008-09-24 | 赵明亮 | Multipurpose microorganism additive agent |
| CN101671212A (en) * | 2009-09-28 | 2010-03-17 | 上海创博现代自然农业（集团）有限公司 | Microbial agent capable of reducing leaf vegetable plant diseases and insect pests and preparing method thereof |
| CN102910988A (en) * | 2012-11-18 | 2013-02-06 | 蒋常德 | Compound microorganism liquid bacterial fertilizer for preventing plant diseases and insect pests and preparation method of compound microorganism liquid bacterial fertilizer |
| CN103911312A (en) * | 2013-09-13 | 2014-07-09 | 领绿生物镇江有限公司 | Microbial preparation with efficacy of purifying water, fertilizing water and caring health and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111149814A (en) * | 2020-01-14 | 2020-05-15 | 河南农贝得农业科技有限公司 | Agricultural microbial agent with insect expelling effect and preparation method thereof |
| CN111543443A (en) * | 2020-06-12 | 2020-08-18 | 江苏益睦生物科技有限公司 | Biological insect-resist agent and preparation method and application thereof |
| CN113693084A (en) * | 2021-08-27 | 2021-11-26 | 烟台凯多海洋生物研究院有限公司 | Fig leaf-derived insect repellent and preparation and application thereof |
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