CN111543443A - Biological insect-resist agent and preparation method and application thereof - Google Patents
Biological insect-resist agent and preparation method and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/26—Meliaceae [Chinaberry or Mahogany family], e.g. mahogany, langsat or neem
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Natural Medicines & Medicinal Plants (AREA)
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Abstract
The invention relates to the technical field of insect-resist agents, in particular to a biological insect-resist agent and a preparation method and application thereof, wherein the biological insect-resist agent is prepared from chinaberry bark, anise, dry hot pepper and fermentation strains, the fermentation strains comprise saccharomycetes, lactic acid bacteria, photosynthetic bacteria and actinomycetes, and per 100kg of fermentation liquor: 0.5-0.8 kg of cortex meliae, 0.5-0.8 kg of anise, 0.5-0.8 kg of dried pepper and 5-6 kg of EM.1 strain; the biological insect-resist agent has the advantages that the raw materials are simple and easy to obtain, and after the biological insect-resist agent is sprayed on the leaf surfaces, the metabolism of plants can be enhanced, the cuticle of the protective film of the leaf can be strengthened, and pathogenic bacteria can be prevented from entering the leaves; in addition, the leavening agent contains ester components, which are not decomposed in the bodies of the herbivorous pests to cause physiological barrier and death, and in addition, due to the action of stimulating substances such as capsaicin and the like, the pests can be dislocated to escape, the pest prevention and removal effect is obvious, and the leavening agent has the advantages of simple preparation process, convenient use, low production cost and suitability for industrial development.
Description
Technical Field
The invention relates to the technical field of insect-resist agents, in particular to a biological insect-resist agent and a preparation method and application thereof.
Background
The disadvantages of chemical pesticide disinfestation are: the increase of residual toxicity, environmental pollution and killing of natural enemies of organisms have certain threats to people and livestock, the balance of organisms is damaged, and pests are easy to generate drug resistance after continuous use, so that biological insect prevention is more and more emphasized by scientific researchers. Biological insect control comprises three modes: birds, insects, and fermented microbial preparations.
The microbial preparation is a product containing active microorganisms, is applied to agricultural production, can provide special nutrients for crops, promotes the metabolism of the crops, and has the effects of bacteriostasis and insect prevention.
Patent 201910215886.4 discloses a biological antibacterial and insect-proof ecological system preparation, its preparation method and application, which is prepared by adding functional bacteria into the Chinese traditional medicine, fruit, vegetable and vegetable wastes respectively, fermenting again, adding carbonized organic substance, wood vinegar, natural mineral elements, agricultural and chitosan oligosaccharide, agricultural trace elements and plant extracts, and is an ecological system preparation suitable for microbial growth and integrating microorganisms, organic active carrier, organic nutrition, trace element nutrition and auxiliary insect-proof plant extracts, and can be rapidly propagated around the rhizosphere after application to form dominant bacteria, thereby playing the roles of antibacterial and insect-proof, continuous cropping resistance, crop growth stimulation, fertilizer utilization rate improvement, crop yield and quality, but the effect is: the beneficial microorganisms have high growth and reproduction capability after being applied to soil, have insect prevention and disease resistance effects on the growth of crops, increase safety, have no harmful substances, improve the safety of agricultural products, and have no other functions except the insect pest releasing effect in the specification, so the biological insect repellent has complex components, high raw material cost and complex and complicated production process flow from the function of the insect repellent.
Disclosure of Invention
The purpose of the invention is: overcomes the defects in the prior art and provides a biological insect-resist agent with simple components and high insect-resist efficiency.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a biological insect-resist agent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.5-0.8 kg of cortex meliae, 0.5-0.8 kg of anise, 0.5-0.8 kg of dried pepper and 5-6 kg of EM.1 strain.
Furthermore, the cortex meliae and the aniseed are mature dry substances, and before fermentation, the cortex meliae, the aniseed and the dry hot pepper are crushed, wherein the crushing grain size is 0.5-1 mm.
Further, the pulverized cortex meliae, star anise and dry hot pepper before fermentation are soaked in clear water.
Further, per 100kg of fermentation broth, the following steps are required: 0.7kg of cortex meliae, 0.6kg of anise, 0.6kg of dried pepper and 5.6kg of EM.1 strain.
Another object of the invention is: provides a preparation method of the biological insect-resist agent with low production cost and simple production process.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows:
a preparation method of a biological insect-resist agent is characterized by comprising the following steps: the preparation method comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
and S3, putting the EM.1 strain into a heat-preservation fermentation tank, and fully fermenting to obtain the compound biological preparation.
Further, in the step S3, the fermentation temperature in the heat-preservation fermentation tank is 25-36 ℃, and the fermentation lasts for 5-7 days.
Further, the fermentation in the step S3 is anaerobic fermentation.
Still another object of the present invention is: provides an application of a biological insect-resist agent in the aspect of preventing and controlling nematodes and pests eating plant stems and leaves.
The technical scheme adopted by the invention has the beneficial effects that:
the biological insect-resist agent has the advantages that the raw materials are simple and easy to obtain, and after the biological insect-resist agent is sprayed on the leaf surfaces, the metabolism of plants can be enhanced, the cuticle of the protective film of the leaf can be strengthened, and pathogenic bacteria can be prevented from entering the leaves; in addition, the leavening agent contains ester components, which are not decomposed in the bodies of the herbivorous pests to cause physiological barrier and death, and in addition, due to the action of stimulating substances such as capsaicin and the like, the pests can be dislocated to escape, the pest prevention and removal effect is obvious, and the leavening agent has the advantages of simple preparation process, convenient use, low production cost and suitability for industrial development.
Detailed Description
The following examples are intended to provide those skilled in the art with a more complete understanding of the present invention, and are not intended to limit the scope of the present invention. Reference herein to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic may be included in at least one implementation of the invention. The appearances of the phrase "in one embodiment" in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments.
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.5-0.8 kg of cortex meliae, 0.5-0.8 kg of anise, 0.5-0.8 kg of dried pepper and 5-6 kg of EM.1 strain. Preferably, per 100kg of fermentation broth: 0.7kg of cortex meliae, 0.6kg of anise, 0.6kg of dried pepper and 5.6kg of EM.1 strain.
The function is as follows: the metabolism of plants is enhanced, the protective film cuticle of the leaves is strengthened, and pathogenic bacteria are prevented from entering the leaves; in addition, the leaven contains ester components, which are not decomposed in the body of the herbivorous pests, and cause physiological barrier to kill the pests.
The preparation method comprises the steps of crushing the chinaberry bark, the star anise and the dry hot pepper to obtain dry substances with the particle size of 0.5-1 mm, wherein the dry substances with the particle size of 0.5-1 mm are crushed before fermentation, so that the dry substances are easier to soak in the soaking process, later-stage fermentation is facilitated, and effective ingredients in the raw materials are fully fermented and utilized, so that the insect prevention efficiency of the biological insect repellent is improved.
The pulverized cortex meliae, fructus Anisi Stellati and dry Capsici fructus before fermentation are soaked in clear water, and after soaking, fermentation conditions are provided for microorganisms, thereby improving fermentation efficiency.
A preparation method of a biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank.
Preferably, the fermentation temperature in the heat-preservation fermentation tank in the step S3 is 25-36 ℃, and the fermentation lasts for 5-7 days.
Preferably, the fermentation in step S3 is an anaerobic fermentation.
The fermentation process has mild conditions, simple steps, high production efficiency and low cost
The biological insect-resist agent is applied to the prevention and control of nematodes and pests eating plant stems and leaves.
Example 1
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.5kg of cortex meliae, 0.5kg of anise, 0.5kg of dried pepper and 5kg of EM.1 strain.
Before fermentation, crushing the cortex meliae, the anise and the dry hot pepper, wherein the crushed grain size is 0.5 mm; soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
The preparation method of the biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank for anaerobic fermentation at the fermentation temperature of 25-36 ℃ for 5 days, and fully fermenting to obtain the compound biological agent.
The using method comprises the following steps: the product is diluted by 500 times with water, and is sprayed on plant leaf surface before insect pest occurs, and both front and back surfaces of the leaf surface are sprayed.
The biological insect-resist agent is suitable for all plants, has obvious control effect on nematodes and pests eating plant stems and leaves, and has the insect-resist control efficiency of 75.7 percent in field tests.
Example 2
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.6kg of cortex meliae, 0.5kg of anise, 0.56kg of dried pepper and 5.5kg of EM.1 strain.
Before fermentation, crushing the cortex meliae, the anise and the dry hot pepper, wherein the crushed grain size is 0.6 mm; soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
The preparation method of the biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank for anaerobic fermentation at the fermentation temperature of 25-36 ℃ for 5 days, and fully fermenting to obtain the compound biological agent.
The using method comprises the following steps: the product is diluted by 800 times with water, and is sprayed on plant leaf surfaces before insect pests occur, and the front and back surfaces of the leaf surfaces are sprayed.
The biological insect-resist agent is suitable for all plants, has obvious control effect on nematodes and pests eating plant stems and leaves, and has the insect pest control efficiency of 76.2 percent in field tests.
Example 3
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.7kg of cortex meliae, 0.6kg of anise, 0.6kg of dried pepper and 5.6kg of EM.1 strain.
The preparation method comprises the following steps of (1) crushing the cortex meliae, the anise and the dry hot pepper into powder, wherein the cortex meliae and the anise are mature dry substances, and the particle size of the crushed powder is 0.5-1 mm before fermentation; soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
The preparation method of the biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank for anaerobic fermentation at the fermentation temperature of 25-36 ℃ for 5-7 days, and fully fermenting to obtain the compound biological agent.
The using method comprises the following steps: the product is diluted by 600 times with water, and sprayed on plant leaf surface before insect pest occurs, and both front and back surfaces of the leaf surface are sprayed.
The biological insect-resist agent is suitable for all plants, has obvious control effect on nematodes and pests eating plant stems and leaves, and has the insect-resist control efficiency of 77.8 percent in field tests.
Example 4
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.7kg of cortex meliae, 0.6kg of anise, 0.7kg of dried pepper and 5.8kg of EM.1 strain.
Before fermentation, crushing the cortex meliae, the anise and the dry hot pepper, wherein the crushed grain size is 0.8 mm; soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
The preparation method of the biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank for anaerobic fermentation at the fermentation temperature of 25-36 ℃ for 5-7 days, and fully fermenting to obtain the compound biological agent.
The using method comprises the following steps: the product is diluted by 600 times with water, and sprayed on plant leaf surface before insect pest occurs, and both front and back surfaces of the leaf surface are sprayed.
The biological insect-resist agent is suitable for all plants, has obvious control effect on nematodes and pests eating plant stems and leaves, and has the insect-resist control efficiency of 77.8 percent in field tests.
Example 5
A biological insect repellent is prepared from cortex Meliae, fructus Anisi Stellati, dried Capsici fructus and fermentation strain including yeast, lactobacillus, photosynthetic bacteria and actinomycetes, wherein per 100kg fermentation liquid: 0.8kg of cortex meliae, 0.8kg of anise, 0.8kg of dried pepper and 6kg of EM.1 strain.
The preparation method comprises the following steps of (1) crushing the cortex meliae, the anise and the dry hot pepper into powder, wherein the cortex meliae and the anise are mature dry substances, and the particle size of the crushed powder is 0.5-1 mm before fermentation; soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
The preparation method of the biological insect-resist agent comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
s3, putting the EM.1 strain into a heat-preservation fermentation tank for anaerobic fermentation at the fermentation temperature of 25-36 ℃ for 5-7 days, and fully fermenting to obtain the compound biological agent.
The using method comprises the following steps: the product is diluted 1000 times with water, and sprayed on plant leaf surface before insect pest occurs, and both front and back surfaces of the leaf surface are sprayed.
The biological insect-resist agent is suitable for all plants, has obvious control effect on nematodes and pests eating plant stems and leaves, and has the insect-resist control efficiency of 78.2 percent in field tests.
In light of the foregoing description of the preferred embodiment of the present invention, many modifications and variations will be apparent to those skilled in the art without departing from the spirit and scope of the invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention. The technical scope of the present invention is not limited to the content of the specification, and must be determined according to the scope of the claims.
Claims (8)
1. A biological insect repellent, which is characterized in that: the biological insect-resist agent is prepared from chinaberry bark, anise, dry hot pepper and fermentation strains, wherein the fermentation strains comprise saccharomycetes, lactic acid bacteria, photosynthetic bacteria and actinomycetes, and each 100kg of fermentation liquor needs: 0.5-0.8 kg of cortex meliae, 0.5-0.8 kg of anise, 0.5-0.8 kg of dried pepper and 5-6 kg of EM.1 strain.
2. The biological insect repellent according to claim 1, wherein: the cortex meliae and the star anise are mature dry substances, and before fermentation, the cortex meliae, the star anise and the dry hot pepper are ground, wherein the ground particle size is 0.5-1 mm.
3. The biological insect repellent according to claim 2, wherein: soaking pulverized cortex Meliae, fructus Anisi Stellati and dried Capsici fructus in clear water before fermentation.
4. The biological insect repellent according to claim 1, wherein: the fermentation liquor per 100kg needs to be as follows: 0.7kg of cortex meliae, 0.6kg of anise, 0.6kg of dried pepper and 5.6kg of EM.1 strain.
5. The method for preparing a biological insect repellent according to any one of claims 1 to 4, wherein: the preparation method comprises the following steps:
s1, preparing raw materials and water according to the proportion, and soaking the raw materials in clear water for 12 hours after the raw materials are crushed;
s2, heating the water temperature in the step S1 to 25 +/-2 ℃;
and S3, putting the EM.1 strain into a heat-preservation fermentation tank, and fully fermenting to obtain the compound biological preparation.
6. The method for preparing biological insect-resist agent according to claim 5, wherein the biological insect-resist agent comprises the following steps: and S3, fermenting for 5-7 days at the fermentation temperature of 25-36 ℃ in the heat-preservation fermentation tank.
7. The method for preparing biological insect-resist agent according to claim 5, wherein the biological insect-resist agent comprises the following steps: the fermentation in step S3 is anaerobic fermentation.
8. The use of the biological insect repellent of claim 1 for controlling nematodes and pests that feed on plant stems and leaves.
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Cited By (1)
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| CN113951283A (en) * | 2021-09-17 | 2022-01-21 | 毛升明 | Fermented pesticide and preparation method and application thereof |
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