CN103964969A - Special culture substrate for traditional Chinese medicinal materials and application of special culture substrate - Google Patents
Special culture substrate for traditional Chinese medicinal materials and application of special culture substrate Download PDFInfo
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- CN103964969A CN103964969A CN201410228080.6A CN201410228080A CN103964969A CN 103964969 A CN103964969 A CN 103964969A CN 201410228080 A CN201410228080 A CN 201410228080A CN 103964969 A CN103964969 A CN 103964969A
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- 239000000758 substrate Substances 0.000 title claims abstract description 60
- 239000000463 material Substances 0.000 title claims abstract description 59
- 241000223259 Trichoderma Species 0.000 claims abstract description 31
- 230000001580 bacterial effect Effects 0.000 claims abstract description 29
- 241000196324 Embryophyta Species 0.000 claims abstract description 17
- 238000000855 fermentation Methods 0.000 claims abstract description 17
- 230000004151 fermentation Effects 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 5
- 239000011159 matrix material Substances 0.000 claims description 26
- 238000002360 preparation method Methods 0.000 claims description 24
- 150000001875 compounds Chemical class 0.000 claims description 18
- 241000894006 Bacteria Species 0.000 claims description 17
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims description 16
- 239000004033 plastic Substances 0.000 claims description 15
- 229920003023 plastic Polymers 0.000 claims description 15
- 238000003756 stirring Methods 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 12
- 150000001413 amino acids Chemical class 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 10
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 10
- 238000002156 mixing Methods 0.000 claims description 10
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 9
- QNBTYORWCCMPQP-JXAWBTAJSA-N (Z)-dimethomorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(Cl)=CC=1)=C/C(=O)N1CCOCC1 QNBTYORWCCMPQP-JXAWBTAJSA-N 0.000 claims description 6
- 239000005761 Dimethomorph Substances 0.000 claims description 6
- 241000233866 Fungi Species 0.000 claims description 6
- 239000005802 Mancozeb Substances 0.000 claims description 6
- 241000222336 Ganoderma Species 0.000 claims description 5
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- 235000012538 ammonium bicarbonate Nutrition 0.000 claims description 5
- 238000013467 fragmentation Methods 0.000 claims description 5
- 238000006062 fragmentation reaction Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002985 plastic film Substances 0.000 claims description 5
- 229920006255 plastic film Polymers 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 210000000582 semen Anatomy 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 5
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 3
- 241000244987 Daiswa polyphylla Species 0.000 claims description 3
- 241000233855 Orchidaceae Species 0.000 claims description 3
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 3
- 239000006013 carbendazim Substances 0.000 claims description 3
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 3
- 241000234280 Liliaceae Species 0.000 claims description 2
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- 229940125904 compound 1 Drugs 0.000 claims description 2
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 claims description 2
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- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a special culture substrate for traditional Chinese medicinal materials and an application of the special culture substrate. The special culture substrate for traditional Chinese medicinal materials is obtained by preparing a mother bacterial strain from trichoderma, preparing a fermentation substrate and preparing the culture substrate; the special substrate can be directly added to a decomposition agent, an organic fertilizer or a biological agent and thus applied to the culture of orchidaceous anoectochilus formosanus, dendrobium officinale, liliaceous polyphylla, lilium brownii, radix tetrastigme and other traditional Chinese medicinal materials symbiotic with the trichoderma. The trichoderma has biological antagonistic activity, and is capable of secreting metabolites and competing with pathogenic bacteria for nutrients and the growing space, and therefore, the growth, reproduction and infection of the pathogenic bacteria can be inhibited and an excellent ecological environment is provided for the growth of crops; the special culture substrate further has the effects of improving the defense capability of the crops to diseases, promoting the growth of seeds, improving the vigor of plants and the like, and also has an excellent effect on disease control in the culture process of the traditional Chinese medicinal materials.
Description
Technical field
The invention belongs to Chinese medicinal materials cultivation field, be specifically related to a kind of Chinese medicinal materials plantation substrate and application.
Background technology
Along with social progress, people more and more profoundly recognize the long-term a large amount of harm of chemical pesticide to ecotope and human health that uses.Biological control is more and more subject to people's attention owing to can effectively overcoming these drawbacks, wherein extremely people's concern of the application of biological antagonist in disease flocking biocontrol.
Biological antagonist refers to the mutual resistance between microorganism, mutually exclusive, even internecine struggle.The advantage of biological antagonist is: 1. good to disease-controlling effect, nontoxic to person poultry safety, free from environmental pollution, and noresidue; 2. disease is killed and wounded to high specificity, do not injure natural enemy and beneficial organism, be conducive to keep balance of nature; 3. raw materials for production and effective constituent belong to natural product, can keep environment sustainable development; 4. many factors and composition play a role jointly, make insect and pathogenic bacteria be difficult to develop immunity to drugs; 5. production technique is simple, and expense is lower than chemical pesticide.In the antagonistic microbe of having found at present, Trichoderma (Trichodermaspp) has good biological and ecological methods to prevent plant disease, pests, and erosion activity.
Trichoderma, in Deuteromycotina, hyphomycetes, hyphomycetales, sticky spore mushroom, is the ubiquitous fungi of a class.Wherein, viride is extensive in distributed in nature, normal saprophytic on timber, seed and plant residue.Viride can produce the multiple bioactive enzyme system that has, as cellulase, chitinase, zytase etc.Viride is one of bacterial strain that cellulase-producing activity is the highest, and the cellulase producing has high degrading activity.Viride is again a kind of resourceful antagonistic microbe, can prevent and treat crop root disease, has important effect in plant pathology biological control.
In recent years, along with the development of the research methods such as genetics, molecular biology, biological chemistry and ecology, research to Trichoderma is deepened continuously, make Trichoderma and meta-bolites thereof at Promoting plant growth, the material absorbing utilization ratio that has additional nutrients, raising crop yield, and strengthen the aspects such as corps diseases defense and there is more wide application prospect, particularly, under the overall background of advocating green agriculture, the large scale application of Trichoderma has become an indispensable part in structure modern times, high-performance bio prevention and elimination of disease and pests system.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicinal materials plantation substrate and application thereof, not only can the Ant agonism by Trichoderma suppress pathogenic bacteria growth, breed and infect, inducing plant produces egodefense ability, also can promote the accumulation of Chinese medicinal materials plant-growth, Effective Component of Chinese Medicine, the disease control in centering the planting of medicinal materials process has good result.
For achieving the above object, the present invention adopts following technical scheme:
A kind of Chinese medicinal materials plantation substrate, be by Trichoderma through bacterial classification female plant prepare, the preparation of fermented substrate and the preparation of cultivation matrix, obtain described Chinese medicinal materials plantation substrate.
Its preparation method comprises the following steps:
1) the female preparation of planting of bacterial classification: get the waste mushroom stick after the wood-decay fungi such as weedtree mushroom culture, black fungus and glossy ganoderma, remove plastics bag, after fragmentation as strain raw material; Add brown sugar, 3-10% wheat bran, 2-8% Semen Maydis powder, 1-7% bicarbonate of ammonia, the 3-15% calcium superphosphate of strain raw material weight 0.5-3%, after stirring in stirrer as bacterial classification compound material; The former powder of Trichoderma spore alive is fully mixed by weight 1:1000-1500 with bacterial classification compound material, with white plastic sealed by film covering fermentation 15-20 days, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, be flocculence or fine and close clump pencil, it is green that color is, and bacterium colony surface obtains the female kind of bacterial classification while being concentric wheel stripe shape; Fermentation condition is: pH value 7-8, CO
2concentration is 0.5%, and culture temperature is 20-28 DEG C, humidity 65-70%;
2) preparation of fermented substrate: get the waste mushroom stick after the wood-decay fungi such as weedtree mushroom culture, black fungus and glossy ganoderma, remove plastics bag, broken rear and calcium superphosphate 100-200:1 mixing and stirring by weight, obtains compound; Plant and compound 1:100-250 mixing and stirring by weight female the bacterial classification after fermentation,, pile fermentation 20-30 days airtight with plastic film, obtain fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add 150-250 times of liquid of dimethomorph mancozeb and amino acid SODm in fermented substrate, mix, making amino acid concentration in matrix is 100-200g/L, and MnZn total amount is 20-40g/L, SODm
'smassfraction is 2-10%; Add again matrix volume 300-600 doubly, the massfraction mould cellulose solution of spring flower bud that is 4%, or matrix volume 400-800 doubly, the massfraction carbendazim solution that is 50%, or matrix volume 500-800 doubly, the massfraction thiophanate_methyl solution that is 70%, mix rear adjustment water content of substrate at 25-30%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Living bacteria count>=10 of Trichoderma in the former powder of step 1) Trichoderma spore alive
9cfu/g.
Described Chinese medicinal materials plantation substrate is directly added in decomposing agent, organic fertilizer or bacteria agent, for the cultivation of Chinese medicinal materials.
Described Chinese medicinal materials is the orchid family Herba Anoectochili roxburghii, Herba Dendrobii, Liliaceae Paris polyphylla, lily, Radix Apioris Fortunei (Radix Lespedezae Buergeri) and other can with the Chinese medicinal materials of Trichoderma symbiosis.
For disease and pest, biological control has following effect to Chinese medicinal materials plantation substrate of the present invention:
(1) antagonistic action: the Trichoderma in matrix of the present invention may, by producing micromolecular microbiotic, macromolecular antibacterial protein or cell-wall degrading enzyme class, suppress growth, the breeding of pathogenic bacteria; Meanwhile, antibacterial protein or the lyase of Trichoderma secretion also can suppress infecting of plant pathogenic fungi.
(2) Competition: the Trichoderma in matrix of the present invention is captured moisture and nutrient by Fast Growth, breeding, take up room, consume oxygen etc., suppress and disturb growth, the breeding of other pathogens in same living environment, for Chinese medicinal materials growth provides good ecotope, be particularly useful for that Chinese medicinal materials is wild fosters.
(3) induction of resistance: the Trichoderma in matrix of the present invention can not only directly suppress the Growth and reproduction of Botrytis cinerea, can also induce host plant to produce defensive raction, makes to do deposits yields IRCM Self-Defence System IRCM and obtains disease resistance.
(4) growth-promoting functions: find through field experiment, use matrix of the present invention can not only control disease and occur, can also increase the length of germination rate, root and seedling and the vigor of plant of seed.
(5) inrichment: matrix of the present invention can effectively decompose, activate and enrichment soil in the nutritive element that has been cured, reduce the mutual antagonism between element, the absorption rate of trace element in raising, make Chinese medicinal materials fully absorb various nutritive substances, be conducive to the raising of Chinese medicinal materials output and quality.
Embodiment
Embodiment mono-
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) the female preparation of planting of bacterial classification: get the waste mushroom stick after weedtree mushroom culture, remove plastics bag, after fragmentation as strain raw material; Add brown sugar, 3% wheat bran, 2% Semen Maydis powder, 1% bicarbonate of ammonia, 3% calcium superphosphate of strain raw material weight 0.5%, after stirring in stirrer as bacterial classification compound material; Trichoderma is lived, and (living bacteria count of contained Trichoderma is 2.9 × 10 to the former powder of spore
9cfu/g) fully mix by weight 1:1000 with bacterial classification compound material, cover and ferment 15 days with white plastic sealed by film, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, be flocculence or fine and close clump pencil, it is green that color is, and bacterium colony surface obtains the female kind of bacterial classification while being concentric wheel stripe shape; Fermentation condition is: pH value 7, CO
2concentration is 0.5%, and culture temperature is 20 DEG C, humidity 65%;
2) preparation of fermented substrate: get the waste mushroom stick after weedtree mushroom culture, remove plastics bag, broken rear and calcium superphosphate 100:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:100 mixing and stirring female the bacterial classification after fermentation,, pile fermentation airtight with plastic film 20 days, obtains fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add 150 times of liquid of dimethomorph mancozeb and amino acid SODm in fermented substrate, mix, making amino acid concentration in matrix is 100g/L, and MnZn total amount is 20g/L, and the massfraction of SODm is 2%; Add the mould cellulose solution of spring flower bud that 300 times of matrix volumes, massfraction are 4% again, mix rear adjustment water content of substrate 25%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Gained Chinese medicinal materials plantation substrate can directly add the cultivation for orchid Herba Anoectochili roxburghii in decomposing agent.
Embodiment bis-
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) the female preparation of planting of bacterial classification: get the waste mushroom stick after weedtree cultivating black fungus, remove plastics bag, after fragmentation as strain raw material; Add brown sugar, 5% wheat bran, 4% Semen Maydis powder, 6% bicarbonate of ammonia, 8% calcium superphosphate of strain raw material weight 1.0%, after stirring in stirrer as bacterial classification compound material; Trichoderma is lived, and (living bacteria count of contained Trichoderma is 3.6 × 10 to the former powder of spore
9cfu/g) fully mix by weight 1:1000 with bacterial classification compound material, cover and ferment 17 days with white plastic sealed by film, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, be flocculence or fine and close clump pencil, it is green that color is, and bacterium colony surface obtains the female kind of bacterial classification while being concentric wheel stripe shape; Fermentation condition is: pH value 7.5, CO
2concentration is 0.5%, and culture temperature is 22 DEG C, humidity 68%;
2) preparation of fermented substrate: get the waste mushroom stick after weedtree cultivating black fungus bacterium mushroom, remove plastics bag, broken rear and calcium superphosphate 150:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:200 mixing and stirring female the bacterial classification after fermentation,, pile fermentation airtight with plastic film 25 days, obtains fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add 200 times of liquid of dimethomorph mancozeb and amino acid SODm in fermented substrate, mix, making amino acid concentration in matrix is 100g/L, and MnZn total amount is 30g/L, and the massfraction of SODm is 6%; Add the carbendazim solution that 400 times of matrix volumes, massfraction are 50% again, mix rear adjustment water content of substrate 26%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Gained Chinese medicinal materials plantation substrate can directly add in organic fertilizer, for the cultivation of liliaceous plant Paris polyphylla.
Embodiment tri-
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) the female preparation of planting of bacterial classification: get the waste mushroom stick after weedtree cultivating ganoderma, remove plastics bag, after fragmentation as strain raw material; Add brown sugar, 10% wheat bran, 8% Semen Maydis powder, 7% bicarbonate of ammonia, 10% calcium superphosphate of strain raw material weight 3%, after stirring in stirrer as bacterial classification compound material; Trichoderma is lived, and (living bacteria count of contained Trichoderma is 3.6 × 10 to the former powder of spore
9cfu/g) fully mix by weight 1:1500 with bacterial classification compound material, cover and ferment 20 days with white plastic sealed by film, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, be flocculence or fine and close clump pencil, it is green that color is, and bacterium colony surface obtains the female kind of bacterial classification while being concentric wheel stripe shape; Fermentation condition is: pH value 8, CO
2concentration is 0.5%, and culture temperature is 28 DEG C, humidity 70%;
2) preparation of fermented substrate: get weedtree and plant the waste mushroom stick after glossy ganoderma, remove plastics bag, broken rear and calcium superphosphate 200:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:250 mixing and stirring female the bacterial classification after fermentation,, pile fermentation airtight with plastic film 30 days, obtains fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add 250 times of liquid of dimethomorph mancozeb and amino acid SODm in fermented substrate, mix, making amino acid concentration in matrix is 200g/L, and MnZn total amount is 40g/L, and the massfraction of SODm is 10%; Add the thiophanate_methyl solution that 600 times of matrix volumes, massfraction are 70% again, mix rear adjustment water content of substrate 30%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Gained Chinese medicinal materials plantation substrate can directly add the cultivation for Chinese medicinal materials Radix Apioris Fortunei (Radix Lespedezae Buergeri) in organic fertilizer.
Chinese medicinal materials plantation substrate of the present invention utilizes Trichoderma as microbial strains, by its secretion metabolite and with pathogenic bacteria competition for nutrients and growing space, suppress pathogenic bacteria growth, breed and infect, for plant growth provides good ecotope; Simultaneously Trichoderma also has and improves the defence capability of crop to disease, promotes seed growth, improves the effects such as plant vigor.
The foregoing is only preferred embodiment of the present invention, all equalizations of doing according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.
Claims (8)
1. a Chinese medicinal materials plantation substrate, is characterized in that: by Trichoderma through bacterial classification female plant prepare, the preparation of fermented substrate and the preparation of cultivation matrix, obtain described Chinese medicinal materials plantation substrate.
2. Chinese medicinal materials plantation substrate according to claim 1, is characterized in that: its preparation method comprises the following steps:
1) the female preparation of planting of bacterial classification: get the waste mushroom stick after weedtree cultivation wood-decay fungi, remove after plastics bag, fragmentation as strain raw material; Add brown sugar, 3-10% wheat bran, 2-8% Semen Maydis powder, 1-7% bicarbonate of ammonia, the 3-15% calcium superphosphate of strain raw material weight 0.5-3%, after stirring in stirrer as bacterial classification compound material; The former powder of Trichoderma spore alive is fully mixed by weight 1:1000-1500 with bacterial classification compound material, with white plastic sealed by film covering fermentation 15-20 days, obtain bacterial classification mother and plant;
2) preparation of fermented substrate: get useless bacterium rod after weedtree cultivation wood-decay fungi, remove plastics bag, broken after with calcium superphosphate 100-200:1 mixing and stirring by weight, obtain compound; Plant and compound 1:100-250 mixing and stirring by weight female the bacterial classification after fermentation,, pile fermentation 20-30 days airtight with plastic film, obtain fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add 150-250 times of liquid of dimethomorph mancozeb and amino acid SODm in fermented substrate, mix; Add again matrix volume 300-600 doubly, the massfraction mould cellulose solution of spring flower bud that is 4%, or matrix volume 400-800 doubly, the massfraction carbendazim solution that is 50%, or matrix volume 500-800 doubly, the massfraction thiophanate_methyl solution that is 70%, mix rear adjustment water content of substrate at 25-30%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved.
3. Chinese medicinal materials is cultivated dedicated substrate according to claim 2, it is characterized in that: described wood-decay fungi comprises mushroom, black fungus and glossy ganoderma.
4. Chinese medicinal materials is cultivated dedicated substrate according to claim 2, it is characterized in that: the condition of fermenting in step 1) is: pH value 7-8, CO
2concentration is 0.5%, and culture temperature is 20-28 DEG C, humidity 65-70%.
5. Chinese medicinal materials is cultivated dedicated substrate according to claim 1, it is characterized in that: living bacteria count>=10 of Trichoderma in the former powder of step 1) Trichoderma spore alive
9cfu/g.
6. Chinese medicinal materials is cultivated dedicated substrate according to claim 1, it is characterized in that: step 3) adds after dimethomorph mancozeb and amino acid SODm solution, in matrix, amino acid concentration is 100-200g/L, and MnZn total amount is 20-40g/L, and the massfraction of SODm is 2-10%.
7. an application for Chinese medicinal materials plantation substrate as claimed in claim 1, is characterized in that: described Chinese medicinal materials plantation substrate is directly added in decomposing agent, organic fertilizer or bacteria agent, for the cultivation of Chinese medicinal materials.
8. the application of Chinese medicinal materials plantation substrate according to claim 7, is characterized in that: described Chinese medicinal materials is the orchid family Herba Anoectochili roxburghii, Herba Dendrobii, Liliaceae Paris polyphylla, lily, Radix Apioris Fortunei (Radix Lespedezae Buergeri) and other can with the Chinese medicinal materials of Trichoderma symbiosis.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105559068A (en) * | 2015-12-31 | 2016-05-11 | 浙江省林业科学研究院 | Composition obtained by fermenting tetrastigma hemsleyanum diels et gilg. through edible and medicinal fungi and preparation method of composition |
| CN105746168A (en) * | 2016-05-13 | 2016-07-13 | 贡享本草（永泰）生物科技有限公司 | Imitated wild cultivation method for roxburgh anoectochilus terminal buds |
| CN105900665A (en) * | 2016-05-18 | 2016-08-31 | 江苏道诚生物科技有限公司 | Method for stereo planting of dendrobium candidum and lucid ganoderma in greenhouse |
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| CN105900665A (en) * | 2016-05-18 | 2016-08-31 | 江苏道诚生物科技有限公司 | Method for stereo planting of dendrobium candidum and lucid ganoderma in greenhouse |
| CN105900665B (en) * | 2016-05-18 | 2018-10-16 | 江苏道诚生物科技有限公司 | A kind of method of dendrobium candidum and ganoderma lucidum solid growth in greenhouse |
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| CN110150093A (en) * | 2019-06-03 | 2019-08-23 | 丽水学院 | A kind of compost and its application for improving polysaccharide content of dendrobium candidum |
| CN111727793A (en) * | 2020-05-22 | 2020-10-02 | 贵州省亚热带作物研究所 | Stereoscopic cultivation method for crude drug tsaoko in karst mountain land |
| CN113229278A (en) * | 2021-04-06 | 2021-08-10 | 浙江大学 | Method for improving amino acid content of anoectochilus formosanus |
| CN113491231A (en) * | 2021-07-01 | 2021-10-12 | 深圳市博林环保工程有限公司 | Planting substrate special for urban roof three-dimensional greening and preparation method thereof |
| CN114467683A (en) * | 2022-01-19 | 2022-05-13 | 忻州师范学院 | Cultivation medium for preventing and treating diseases of root-stem medicinal materials and preparation method thereof |
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