CN102503645B - Nutrient medium for planting ganoderma lucidum - Google Patents
Nutrient medium for planting ganoderma lucidum Download PDFInfo
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- CN102503645B CN102503645B CN201110343617.XA CN201110343617A CN102503645B CN 102503645 B CN102503645 B CN 102503645B CN 201110343617 A CN201110343617 A CN 201110343617A CN 102503645 B CN102503645 B CN 102503645B
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- tea oil
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- glossy ganoderma
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The invention provides a nutrient medium for planting ganoderma lucidum, the invention aims at providing a nutrient medium capable of facilitating the production of medical ganoderma lucidum with high quality and high value and protecting the forest resources so as to solve the increasingly serious fungus and forest conflict problem in the development of medical ganoderma lucidum industry; the technical scheme is as follows: the nutrient medium comprises the following raw materials in percentage by weight: 5-90% of tea tree residue, 0-70% of saw dust, 8-18% of wheat bran, 0-5% of corn flour, 1% of sugar and 1% of gypsum; and the invention belongs to the technical field of biological medium.
Description
Technical field
The present invention relates to a kind of nutrition base, relate in particular to a kind of nutrition base that glossy ganoderma is used of planting, belong to biological medium technical field.
Background technology
Increasingly in short supply in resources such as soil, grains, ecotope constantly worsens, today that human health care's consciousness strengthens gradually, robust fibre can be converted into high-quality tropina, to human body, have the food of fungi of unique nourishing function increasingly to be favored, mushroom industry has become the model of green, environmental protection, efficient, circular agriculture.
So far, Ganoderma preparation is raw material mainly with sporophore or solid culture mycelia, and the production cycle is long, is subject to environmental pollution, certainly its quality is exerted an influence.Liquid culture technology, in the application in this field, has realized the heavy industrialization of glossy ganoderma and has cultivated.But send regret, in existing liquid culture technology, substratum all enters culture tank, substratum contains insoluble solid, in liquid culture process, discharge ferment, cause nutrient solution to have peculiar smell, and cause the separation difficulty of mycelia, insoluble solid and nutrient solution; In addition, the most of method that adopts cultivation on sawdust and cultivation basswood of cultivation of glossy ganoderma, need to consume a large amount of timber resources; be unfavorable for the protection of ecotope; extensive development cultivation on sawdust and cultivation basswood Edible Fungi, be subject to forest reserves restriction, and therefore " bacterium woods contradiction " is more and more outstanding
Tea oil tree is the dungarunga of mutual leaf white spirit layer (Melaleuca alternifonia) Myrtaceae white spirit layer platymiscium, originates in New South Wales, Australia region following the line of the sea, among the people be commonly used to anti-infective, anti-inflammatory and treatment diarrhoea and wound.From the fresh branches and leaves of mutual leaf white spirit layer, extract the tea tree oil (Tea Tree Oil, TTO) obtaining, belong to white spirit layer terpin type spice oil, main component is loose alkene hydrocarbon.Tea tree oil tool is antiviral, antibiotic, immuno-stimulating, expelling parasite desinsection, reduce phlegm, the effect such as calmness, pain relieving, promotion wound healing, be one of the most effective natural antibacterial agent of finding up to now, started to be widely used in the industries such as medical and health, daily-use chemical industry, spices, food, agricultural chemicals.Test-results shows, to the streptococcus aureus of more common skin infections, micrococci, enterococcus faecalis, Pseudomonas aeruginosa and Candida albicans etc., the MIC of tea tree oil is no more than 2%.Its anti-microbial activity, far away higher than traditional Chinese medicine antiseptic, even can reach the bactericidal levels of middle effect disinfectants.These series product belong to pure natural and extract, nontoxic, harmless, to people, animal blandness, noresidue, little to plant soil harm, meet the social orientation of " green living, energy-conserving and environment-protective ", meet the requirement of modern's health environment-friendly, there is good pharmaceutical use and industrial value.
In June, 2010, tea oil tree essential oil successfully refines in bio tech ltd, Fuyang, Guangdong, through inspection center of South China Normal University, detecting indices meets international standards, outside the tea tree ethereal oil and Zhongshan University's R & D Cooperation bio-pharmaceuticals extracting, tea oil tree tree slag plantation edible mushrooms technology is is successfully researched and developed in the tea oil tree Shu Zhayeyu Zhongshan University cooperation of producing.Reach 6000 mu to company in 2012 the tea oil tree area of can gathering, can adopt to obtain more than 15000 ton of biomass, can refine 150 tons of tea tree ethereal oils, the crude oil output value can 3,000 ten thousand yuan, and corresponding 12000 tons of the tea oil tree tree slags that will produce will be produced and furnish ample material for development tea oil tree tree slag edible fungus industrial.
Summary of the invention
The object of the invention is for above-mentioned deficiency, provide a kind of tea oil tree tree slag that utilizes to replace wood chip as the nutrition base of plantation medicinal lucid ganoderma.
A kind of nutrition base that glossy ganoderma is used of planting of the present invention, this nutrition base comprises the raw material of following weight per-cent: tea oil tree tree slag 5～90%, wood chip 0～70%, wheat bran 8～18%, Semen Maydis powder 0～5%, sugar 1%, gypsum 1%.
Above-mentioned a kind of nutrition base that glossy ganoderma is used of planting, described nutrition base comprises the raw material of following weight per-cent: slag tea oil tree tree 20～70%, wood chip 5～55%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%.
Above-mentioned a kind of nutrition base that glossy ganoderma is used of planting, described nutrition base comprises the raw material of following weight per-cent: slag tea oil tree tree 30～60%, wood chip 15～45%, wheat bran 8%, Semen Maydis powder 5%, sugar 1%, gypsum 1%.
Above-mentioned a kind of nutrition base that glossy ganoderma is used of planting, described nutrition base pH value is 7～7.5.
Further, above-mentioned a kind of nutrition base that glossy ganoderma is used of planting, citric acid or lime powder regulate for described pH value.
Further, above-mentioned a kind of nutrition base that glossy ganoderma is used of planting, described tea oil tree tree slag is to extract the tea oil tree tree slag after tea oil by boiling mode.
Of the present invention have a following beneficial effect:
1. utilize tea oil tree tree slag to replace wood chip and linden as nutrition base plantation glossy ganoderma, the object that reach recycle, turns waste into wealth; So both be conducive to produce medicinal lucid ganoderma high-quality, high value, and can have played the effect protecting forest resources again, solved day by day serious " bacterium woods contradiction " problem in development mushroom industry.
2. by boiling, extracted the tea oil tree tree slag for cultivating medicinal lucid ganoderma of essential oil, aseptic because of the tree slag through high temperature steaming, do not need the sterilization through pharmaceutical chemicals to can be used to cultivate glossy ganoderma, completely environmental and ecological; The a small amount of tea tree oil also containing in tree slag, is natural bactericidal agent, can kill other contaminated bacterias in glossy ganoderma process of growth, in the process of plantation glossy ganoderma, does not need to spray insecticide again; More loosening through boiling slag wooden structures and wood cell, its nutritive ingredient is easily absorbed by glossy ganoderma, to produce glossy ganoderma output high quality good.
Embodiment
Below in conjunction with the concrete formula of implementing, the present invention is described in further details, but becomes any limitation of the invention not.
Embodiment 1
1. the compound method of culture material
(1) weighing: ready raw material is taken in the definite ratio of formula; Wherein, usedly tea oil tree is set to slag with pulverizer, pulverize.
(2) spice: during preparation, tea oil tree tree slag and water-fast auxiliary material wheat bran, rice bran, wood chip etc. stir, and the form that the water-soluble materials such as sugar, starch is mixed with to the aqueous solution adds.Stir, through repeatedly turning, allow culture material fully absorb moisture.
(3) regulate water content: fine day water evaporation quantity is large, moisture should omit high, and the cloudy day should be on the low side; Spice place water-absorbent is strong, and moisture should be heightened, otherwise should turn down.During with tea oil tree tree slag culture material spice, with hand, pick up a raw material of mixing, holding water can ooze out from finger seam, drips and while not becoming line, water content is 65% left and right; Only have washmarking to occur, water content is 60% left and right.In addition, material must be in a huddle after mixing, and allows moisture fully infiltrate raw material.
(4) measure pH value: material is mixed afterwards and measured its pH with pH test paper, and general pH 7～7.5 is advisable.If meta-alkali, regulates with citric acid; If meta-acid, regulates with lime powder.
2. pack sterilizing
Press culture medium prescription, raw and auxiliary material is got ready, add water and stir, then pack.Adopt polyacrylic film bag (long 40～50cm, wide 22～24cm, 0.05～0.06 centimetre of thickness).
(1) pack
Allow sack two go out glossy ganoderma, before charging, first with mouthful circle and paper, rope, tie sack one end.First grab 2～3 culture material is put in bag, with the corner at the bottom of handgrip bag, be pressed in bag, and compress culture material and make it into cylindrically, a bag end, steadily can stand on ground.When filling with substance elasticity is suitable, and charging limit, general limit is real with hand, while being filled to from sack 5～6cm, stops charging, by the light applanation surface of have gentle hands, makes a call to a hole, then seal sack with the sharp wooden stick of diameter 2.5cm in the middle of Bag Material.The unsuitable tension of when filling with substance, after too tight sterilizing, sack is easily heated and bursts, and ventilation property is too poor, and mycelial growth is too slow; Too pine, crosses loose metal and leaves many spaces with bag wall, causes nutrition waste.Preferably after charging, sack is straight and upright and not crooked, and it is tighter than middle outline that two material fills.In handling process, be difficult for to stop up like this, during sterilizing, steam easily penetrates culture material, and sterilizing can be more thorough, and at the bottom of original seed falls into hole during inoculation, accelerates mycelial growth, shortens Culture spawn incubation time.After culture material installs, sack and surface are cleaned, put the duroplasts collar on sack, allow sack film pass through in encircling, and outwards along ring wall, overturn down, then, by sack leveling, tampon, enters a pot sterilizing beyond the Great Wall.
(2) sterilizing
The pocket installing should not be put for a long time, should within 4h, carry out sterilizing.High temperature season particularly, be especially noted that otherwise substratum at high temperature, microbial reproduction is rapid, can make very soon culture material become sour.Sterilizing adopts free flowing steam sterilization method, with the vapor pressure sterilizing of 1.5 kg/cm 1.5 hours, or atmospheric steam sterilizing 8～10 hours, after sterilizing, take out cooling.When pocket enters sterilizing storehouse, should pile and put well, too not crowded, leave steam channel, allow steam have room of manoeuvre in steaming storehouse, avoid causing the dead angle of sterilizing.
(3) inoculation and cultivation
Cultivar should, by aseptic technique requirement inoculation in sterile cupboard or sterilisable chamber, then be cultivated with original seed similarity condition.
Embodiment 2
The glossy ganoderma speed of growth contrast of different ingredients nutrition base, in the conventional nutrition base in this area, sugared conventional amount used scope is 0.5～3%, and the conventional amount used scope of gypsum is 1～3%, for ease of contrast, in the following embodiment of the present invention, the consumption of fixing sugar and gypsum is 1%.
Test conditions: cultivate base temperature: 21～25 ℃, humidity 82%～93%;
Respectively fill a prescription (proportioning of each raw material is weight percentage) specific as follows of plantation medicinal lucid ganoderma, preparation method is referring to embodiment 1.
Formula 1: wood chip 75%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 2: tea oil tree tree slag 5%, wood chip 70%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 3: tea oil tree tree slag 20%, wood chip 55%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 4: tea oil tree tree slag 30%, wood chip 45%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 5: tea oil tree tree slag 40%, wood chip 35%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 6: tea oil tree tree slag 50%, wood chip 25%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 7: tea oil tree tree slag 60%, wood chip 15%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 8: tea oil tree tree slag 70%, wood chip 5%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Formula 9: tea oil tree tree slag 80%, wheat bran 18%, sugar 1%, gypsum 1%;
Formula 10: tea oil tree tree slag 90%, wheat bran 8%, sugar 1%, gypsum 1%;
Test-results: the cycle of growing that different ingredients bottle plant to be processed and speed is in Table 1, different culture media formula glossy ganoderma ultimate production analysis in table 2.
Table 1 different ingredients bag is planted the cycle of growing and the speed of processing
Table 2 different culture media formula medicinal lucid ganoderma ultimate production is analyzed
From table 1, table 2, can find out, former base divergaence time while adopting culture medium culturing medicinal lucid ganoderma disclosed in this invention, the first damp mushroom time are significantly shorter than former base divergaence time, the first damp mushroom time of the substratum while not adding tea oil tree tree slag, the long speed of mycelia, ultimate production, biological efficiency former base differentiation mycelia length speed, ultimate production, the biological efficiency when not adding tea oil tree tree slag.
Embodiment 3
The bacteriostatic experiment of substratum
In employing agar diffusion method of the paper mensuration embodiment 2, substratum 1 is to the fungistatic effect of 8 pairs of escherichia colis of substratum, streptococcus aureus, the false silk of white bacteria culture fluid, in Table 5.
Table 3 fungistatic effect
Note: * indicate that, without fungistatic effect, √ indicates fungistatic effect.
Claims (5)
1. plant the nutrition base that glossy ganoderma is used, it is characterized in that, described nutrition base comprises the raw material of following weight per-cent: tea oil tree tree slag 20～70%, wood chip 5～55%, wheat bran 18%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Wherein: described tea oil tree tree slag is to extract the tea oil tree tree slag after tea oil by boiling mode.
2. plant the nutrition base that glossy ganoderma is used, it is characterized in that, described nutrition base comprises the raw material of following weight per-cent: tea oil tree tree slag 30～60%, wood chip 15～45%, wheat bran 8%, Semen Maydis powder 5%, sugar 1%, gypsum 1%;
Wherein: described tea oil tree tree slag is to extract the tea oil tree tree slag after tea oil by boiling mode.
3. according to right, want 1 to 2 arbitrary described a kind of nutrition base that glossy ganoderma is used of planting, it is characterized in that, described nutrition base pH value is 7～7.5.
4. according to right, want a kind of described in 3 to plant the nutrition base that glossy ganoderma is used, it is characterized in that, described pH value regulates with citric acid.
5. according to right, want a kind of described in 3 to plant the nutrition base that glossy ganoderma is used, it is characterized in that, described pH value regulates with lime powder.
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| CN104030848B (en) * | 2014-06-30 | 2016-01-20 | 桂林丰茂源农业技术开发有限公司 | A kind of glossy ganoderma substituting stuff cultivation substratum that with the addition of Flower of Chinese Globeflower |
| CN104756760A (en) * | 2015-03-18 | 2015-07-08 | 金寨金芝源生物工程有限公司 | Selenium-enriched ganoderma lucidum cultivation material and preparation method thereof |
| CN105884463A (en) * | 2016-05-25 | 2016-08-24 | 广州聚注专利研发有限公司 | Nutrient medium for lucid ganoderma cultivation |
| CN106242750A (en) * | 2016-07-29 | 2016-12-21 | 浦北县健翔食品厂 | A kind of Ganoderma substituting stuff cultivation culture medium that can suppress rhizopus stolonifer |
| CN106242749A (en) * | 2016-07-29 | 2016-12-21 | 浦北县健翔食品厂 | A kind of Ganoderma substituting stuff cultivation culture medium that can suppress butt Hypoxylon |
| CN106631344A (en) * | 2016-09-30 | 2017-05-10 | 桂林淮安天然保健品开发有限公司 | Lucid ganoderma bag cultivation culture medium capable of increasing content of ganoderan and selenium |
| CN112493054A (en) * | 2020-11-23 | 2021-03-16 | 安徽农业大学 | Ganoderma lucidum culture material based on moso bamboo sawdust and preparation method and application thereof |
| CN115918509B (en) * | 2022-11-29 | 2023-08-18 | 广州汉方医学生物科技有限公司 | Culture method and application of dendrobium candidum |
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| CN1257856A (en) * | 1998-12-24 | 2000-06-28 | 中国科学院长沙大地构造研究所 | Process for preparing fertilizer used to cultivate straw mushroom |
| CN1231336A (en) * | 1999-01-30 | 1999-10-13 | 隋进清 | Bag-cultivating black edible fungus and cultivating technology thereof |
| CN101450879A (en) * | 2007-11-29 | 2009-06-10 | 姜堰市现代农业科技实验场 | Mushroom cultivation substrate |
| CN101723759B (en) * | 2009-10-30 | 2012-07-25 | 山东芳绿农业科技有限公司 | Black fungus cultivation method and cultivation material thereof |
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