CN103964969B - A kind of Chinese medicinal materials plantation substrate and application thereof - Google Patents

A kind of Chinese medicinal materials plantation substrate and application thereof Download PDF

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CN103964969B
CN103964969B CN201410228080.6A CN201410228080A CN103964969B CN 103964969 B CN103964969 B CN 103964969B CN 201410228080 A CN201410228080 A CN 201410228080A CN 103964969 B CN103964969 B CN 103964969B
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medicinal materials
chinese medicinal
substrate
trichoderma
bacterial classification
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CN103964969A (en
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陈敏健
唐建阳
周大顺
周先治
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STRAIT (XIAMEN) MODERN AGRICULTURAL RESEARCH INSTITUTE Co Ltd
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STRAIT (XIAMEN) MODERN AGRICULTURAL RESEARCH INSTITUTE Co Ltd
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Abstract

The invention discloses a kind of Chinese medicinal materials plantation substrate and application, be Trichoderma is planted through bacterial classification mother prepare, the preparation of fermented substrate and the preparation of cultivation matrix, obtain described Chinese medicinal materials plantation substrate, this matrix can directly add in decomposing agent, organic fertilizer or bacteria agent for the orchid family Herba Anoectochili roxburghii, Herba Dendrobii, Liliaceae Paris polyphylla, lily, Radix Apioris Fortunei (Radix Lespedezae Buergeri) and other can cultivate with the Chinese medicinal materials of Trichoderma symbiosis.Utilize the biological antagonist of Trichoderma active, by its secretion metabolite and with pathogenic bacteria competition for nutrients and growing space, suppress the growth of pathogenic bacteria, breed and infect, for plant growth provides good ecotope; Also have and improve the effect such as defence capability, promotion seed growth, raising plant vigor of crop to disease, the disease control in centering the planting of medicinal materials process has good result.

Description

A kind of Chinese medicinal materials plantation substrate and application thereof
Technical field
The invention belongs to Chinese medicinal materials cultivation field, be specifically related to a kind of Chinese medicinal materials plantation substrate and application.
Background technology
Along with the progress of society, people more and more profoundly recognize that the long-term a large amount of chemical pesticide that uses is to the harm of ecotope and human health.Biological control is more and more subject to people's attention owing to can effectively overcome these drawbacks, the wherein application of biological antagonist in disease flocking biocontrol extremely people's concern.
Biological antagonist refers to the mutual resistance between microorganism, mutually exclusive, even internecine struggle.The advantage of biological antagonist is: 1. good to disease-controlling effect, nontoxic to person poultry safety, free from environmental pollution, noresidue; 2. high specificity is killed and wounded to disease, do not injure natural enemy and beneficial organism, be conducive to keeping balance of nature; 3. raw materials for production and effective constituent belong to natural product, can keep environment sustainable development; 4. many factors and composition play a role jointly, make insect and pathogenic bacteria be difficult to develop immunity to drugs; 5. production technique is simple, and expense is lower than chemical pesticide.In the antagonistic microbe found at present, it is active that Trichoderma (Trichodermaspp) has good biological and ecological methods to prevent plant disease, pests, and erosion.
Trichoderma, in Deuteromycotina, hyphomycetes, hyphomycetales, sticky spore mushroom, is the ubiquitous fungi of a class.Wherein, viride is extensive in distributed in nature, often saprophytic on timber, seed and plant residue.Viride can produce multiplely has bioactive enzyme system, as cellulase, chitinase, zytase etc.Viride is one of cellulase-producing the highest active bacterial strain, and the cellulase produced has high degrading activity.Viride is again a kind of resourceful antagonistic microbe, can prevent and treat crop root disease, in plant pathology biological control, have important effect.
In recent years, along with the development of the research methods such as genetics, molecular biology, biological chemistry and ecology, the research of Trichoderma is deepened continuously, make Trichoderma and meta-bolites thereof in Promoting plant growth, the material absorbing utilization ratio that has additional nutrients, improve crop yield, and strengthen the aspects such as corps diseases is defensive there is more wide application prospect, particularly under the overall background advocating green agriculture, the large scale application of Trichoderma has become a part indispensable in structure modern times, high-performance bio prevention and elimination of disease and pests system.
Summary of the invention
The object of the present invention is to provide a kind of Chinese medicinal materials plantation substrate and application thereof, Ant agonism not only by Trichoderma suppresses the growth of pathogenic bacteria, breeds and infect, inducing plant produces egodefense ability, also can promote the accumulation of Chinese medicinal materials plant-growth, Effective Component of Chinese Medicine, the disease control in centering the planting of medicinal materials process has good result.
For achieving the above object, the present invention adopts following technical scheme:
A kind of Chinese medicinal materials plantation substrate, be Trichoderma is planted through bacterial classification mother prepare, the preparation of fermented substrate and the preparation of cultivation matrix, obtain described Chinese medicinal materials plantation substrate.
Its preparation method comprises the following steps:
1) preparation of bacterial classification mother kind: get the waste mushroom stick after the wood-decay fungi such as weedtree mushroom culture, black fungus and glossy ganoderma, remove plastics bag, as strain raw material after fragmentation; Add the brown sugar of strain raw material weight 0.5-3%, 3-10% wheat bran, 2-8% Semen Maydis powder, 1-7% bicarbonate of ammonia, 3-15% calcium superphosphate, as bacterial classification compound material after stirring in stirrer; Original spore powder of being lived by Trichoderma fully mixes by weight 1:1000-1500 with bacterial classification compound material, with White plastic film airtight covering fermentation 15-20 days, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, in flocculence or fine and close clump pencil, color, in green, obtain bacterial classification mother and plants when bacterium colony surface is in concentric wheel stripe shape; Fermentation condition is: pH value 7-8, CO 2concentration is 0.5%, and culture temperature is 20-28 DEG C, humidity 65-70%;
2) preparation of fermented substrate: get the waste mushroom stick after the wood-decay fungi such as weedtree mushroom culture, black fungus and glossy ganoderma, removes plastics bag, and broken rear and calcium superphosphate 100-200:1 mixing and stirring by weight, obtains compound; Plant the bacterial classification mother after fermentation with compound by weight 1:100-250 mixing and stirring, pile fermentation 20-30 days airtight with plastic film, obtains fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add dimethomorph mancozeb and amino acid SODm150-250 times liquid in fermented substrate, mixing, make amino acid concentration in matrix be 100-200g/L, MnZn total amount is 20-40g/L, SODm 'smassfraction is 2-10%; Add again matrix volume 300-600 doubly, massfraction be 4% the mould cellulose solution of spring flower bud, or matrix volume 400-800 doubly, massfraction is the carbendazim solution of 50%, or matrix volume 500-800 doubly, massfraction be 70% thiophanate_methyl solution, mix rear adjustment water content of substrate at 25-30%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Living bacteria count>=10 of Trichoderma in step 1) Trichoderma original spore powder alive 9cfu/g.
Described Chinese medicinal materials plantation substrate is directly added in decomposing agent, organic fertilizer or bacteria agent, for the cultivation of Chinese medicinal materials.
Described Chinese medicinal materials is the orchid family Herba Anoectochili roxburghii, Herba Dendrobii, Liliaceae Paris polyphylla, lily, Radix Apioris Fortunei (Radix Lespedezae Buergeri) and other can with the Chinese medicinal materials of Trichoderma symbiosis.
Chinese medicinal materials plantation substrate of the present invention has following effect for disease and pest biological control:
(1) antagonistic action: the Trichoderma in matrix of the present invention by producing micromolecular microbiotic, macromolecular antibacterial protein or cell-wall degrading enzyme class, can suppress the growth of pathogenic bacteria, breeding; Meanwhile, Trichoderma secretion antibacterial protein or lyase also can suppress infecting of plant pathogenic fungi.
(2) Competition: the Trichoderma in matrix of the present invention is by growth fast, breeding deprives moisture and nutrient, take up room, consume oxygen etc., suppress and disturb growth, the breeding of other pathogens in same living environment, for Chinese medicinal materials growth provides good ecotope, be particularly useful for Chinese medicinal materials natural cultivation.
(3) induction of resistance: the Trichoderma in matrix of the present invention directly can not only suppress the Growth and reproduction of Botrytis cinerea, can also induce host plant to produce defensive raction, makes crop produce IRCM Self-Defence System IRCM and obtains disease resistance.
(4) growth-promoting functions: find through field experiment, uses matrix of the present invention can not only control disease and occurs, can also increase the length of the germination rate of seed, root and seedling and the vigor of plant.
(5) inrichment: matrix of the present invention can effectively be decomposed, activate and the nutritive element that has been cured in Enriching soil, reduce the mutual antagonism between element, improve the absorption rate of medium trace element, make Chinese medicinal materials fully absorb various nutritive substance, be conducive to the raising of Chinese medicinal materials seed output and quality.
Embodiment
Embodiment one
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) preparation of bacterial classification mother kind: get the waste mushroom stick after weedtree mushroom culture, remove plastics bag, as strain raw material after fragmentation; Add the brown sugar of strain raw material weight 0.5%, 3% wheat bran, 2% Semen Maydis powder, 1% bicarbonate of ammonia, 3% calcium superphosphate, as bacterial classification compound material after stirring in stirrer; Being lived by Trichoderma, (living bacteria count of contained Trichoderma is 2.9 × 10 to original spore powder 9cfu/g) fully mix by weight 1:1000 with bacterial classification compound material, with the airtight covering fermentation of White plastic film 15 days, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, in flocculence or fine and close clump pencil, color, in green, obtain bacterial classification mother and plants when bacterium colony surface is in concentric wheel stripe shape; Fermentation condition is: pH value 7, CO 2concentration is 0.5%, and culture temperature is 20 DEG C, humidity 65%;
2) preparation of fermented substrate: get the waste mushroom stick after weedtree mushroom culture, removes plastics bag, and broken rear and calcium superphosphate 100:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:100 mixing and stirring by the bacterial classification mother after fermentation again, pile fermentation airtight with plastic film 20 days, obtains fermented substrate for subsequent use;
3) preparation of cultivation matrix: before cultivation, add dimethomorph mancozeb and amino acid SODm150 times liquid in fermented substrate, mixing, make amino acid concentration in matrix be 100g/L, MnZn total amount is the massfraction of 20g/L, SODm is 2%; Add matrix volume 300 times again, massfraction be 4% the mould cellulose solution of spring flower bud, mix rear adjustment water content of substrate 25%, obtain described Chinese medicinal materials plantation substrate, pack sealing preserve, 90 days can be stored under normal temperature.
Gained Chinese medicinal materials plantation substrate directly can add the cultivation for orchid Herba Anoectochili roxburghii in decomposing agent.
Embodiment two
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) preparation of bacterial classification mother kind: get the waste mushroom stick after weedtree cultivating black fungus, remove plastics bag, as strain raw material after fragmentation; Add the brown sugar of strain raw material weight 1.0%, 5% wheat bran, 4% Semen Maydis powder, 6% bicarbonate of ammonia, 8% calcium superphosphate, as bacterial classification compound material after stirring in stirrer; Being lived by Trichoderma, (living bacteria count of contained Trichoderma is 3.6 × 10 to original spore powder 9cfu/g) fully mix by weight 1:1000 with bacterial classification compound material, with the airtight covering fermentation of White plastic film 17 days, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, in flocculence or fine and close clump pencil, color, in green, obtain bacterial classification mother and plants when bacterium colony surface is in concentric wheel stripe shape; Fermentation condition is: pH value 7.5, CO 2concentration is 0.5%, and culture temperature is 22 DEG C, humidity 68%;
2) preparation of fermented substrate: get the waste mushroom stick after weedtree cultivating black fungus bacterium mushroom, removes plastics bag, and broken rear and calcium superphosphate 150:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:200 mixing and stirring by the bacterial classification mother after fermentation again, pile fermentation airtight with plastic film 25 days, obtains fermented substrate for subsequent use;
3) preparation of cultivation matrix: before cultivation, add dimethomorph mancozeb and amino acid SODm200 times liquid in fermented substrate, mixing, make amino acid concentration in matrix be 100g/L, MnZn total amount is the massfraction of 30g/L, SODm is 6%; Add matrix volume 400 times again, massfraction is the carbendazim solution of 50%, mix rear adjustment water content of substrate 26%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved, and can store 90 days under normal temperature.
Gained Chinese medicinal materials plantation substrate can directly add in organic fertilizer, for the cultivation of liliaceous plant Paris polyphylla.
Embodiment three
A kind of Chinese medicinal materials plantation substrate, its preparation method comprises the following steps:
1) preparation of bacterial classification mother kind: get the waste mushroom stick after weedtree cultivating ganoderma, remove plastics bag, as strain raw material after fragmentation; Add the brown sugar of strain raw material weight 3%, 10% wheat bran, 8% Semen Maydis powder, 7% bicarbonate of ammonia, 10% calcium superphosphate, as bacterial classification compound material after stirring in stirrer; Being lived by Trichoderma, (living bacteria count of contained Trichoderma is 3.6 × 10 to original spore powder 9cfu/g) fully mix by weight 1:1500 with bacterial classification compound material, with the airtight covering fermentation of White plastic film 20 days, spore is with mucus conglomerate or subglobose spore head, bacterium colony stretches rapidly, in flocculence or fine and close clump pencil, color, in green, obtain bacterial classification mother and plants when bacterium colony surface is in concentric wheel stripe shape; Fermentation condition is: pH value 8, CO 2concentration is 0.5%, and culture temperature is 28 DEG C, humidity 70%;
2) preparation of fermented substrate: get the waste mushroom stick after weedtree cultivation glossy ganoderma, remove plastics bag, broken rear and calcium superphosphate 200:1 mixing and stirring by weight, obtains compound; Plant with compound by weight 1:250 mixing and stirring by the bacterial classification mother after fermentation again, pile fermentation airtight with plastic film 30 days, obtains fermented substrate for subsequent use;
3) preparation of cultivation matrix: before cultivation, add dimethomorph mancozeb and amino acid SODm250 times liquid in fermented substrate, mixing, make amino acid concentration in matrix be 200g/L, MnZn total amount is the massfraction of 40g/L, SODm is 10%; Add matrix volume 600 times again, massfraction be 70% thiophanate_methyl solution, mix rear adjustment water content of substrate 30%, obtain described Chinese medicinal materials plantation substrate, pack sealing preserve, 90 days can be stored under normal temperature.
Gained Chinese medicinal materials plantation substrate directly can add the cultivation for Chinese medicinal materials Radix Apioris Fortunei (Radix Lespedezae Buergeri) in organic fertilizer.
Chinese medicinal materials plantation substrate of the present invention utilizes Trichoderma as microbial strains, by its secretion metabolite and with pathogenic bacteria competition for nutrients and growing space, suppress pathogenic bacteria growth, breed and infect, for plant growth provides good ecotope; Trichoderma also has the effect such as defence capability, promotion seed growth, raising plant vigor of raising crop to disease simultaneously.
The foregoing is only preferred embodiment of the present invention, all equalizations done according to the present patent application the scope of the claims change and modify, and all should belong to covering scope of the present invention.

Claims (7)

1. a Chinese medicinal materials plantation substrate, is characterized in that: Trichoderma is planted through bacterial classification mother prepare, the preparation of fermented substrate and the preparation of cultivation matrix, obtain described Chinese medicinal materials plantation substrate;
Its preparation method comprises the following steps:
1) preparation of bacterial classification mother kind: get the waste mushroom stick after weedtree cultivation wood-decay fungi, as strain raw material after removing plastics bag, fragmentation; Add the brown sugar of strain raw material weight 0.5-3%, 3-10% wheat bran, 2-8% Semen Maydis powder, 1-7% bicarbonate of ammonia, 3-15% calcium superphosphate, as bacterial classification compound material after stirring in stirrer; Original spore powder of being lived by Trichoderma fully mixes by weight 1:1000-1500 with bacterial classification compound material, with White plastic film airtight covering fermentation 15-20 days, obtains bacterial classification mother and plants;
2) preparation of fermented substrate: get the bacterium rod given up after wood-decay fungi cultivated by weedtree, removes plastics bag, broken rear and calcium superphosphate 100-200:1 mixing and stirring by weight, obtains compound; Plant the bacterial classification mother after fermentation with compound by weight 1:100-250 mixing and stirring, pile fermentation 20-30 days airtight with plastic film, obtains fermented substrate for subsequent use again;
3) preparation of cultivation matrix: before cultivation, add dimethomorph mancozeb and amino acid SODm150-250 times liquid in fermented substrate, mixing; Add again matrix volume 300-600 doubly, massfraction be 4% the mould cellulose solution of spring flower bud, or matrix volume 400-800 doubly, massfraction is the carbendazim solution of 50%, or matrix volume 500-800 doubly, massfraction be 70% thiophanate_methyl solution, mix rear adjustment water content of substrate at 25-30%, obtain described Chinese medicinal materials plantation substrate, pack sealing is preserved.
2. Chinese medicinal materials plantation substrate according to claim 1, is characterized in that: described wood-decay fungi comprises mushroom, black fungus and glossy ganoderma.
3. Chinese medicinal materials plantation substrate according to claim 1, is characterized in that: the condition of fermenting in step 1) is: pH value 7-8, CO 2concentration is 0.5%, and culture temperature is 20-28 DEG C, humidity 65-70%.
4. Chinese medicinal materials plantation substrate according to claim 1, is characterized in that: step 1) Trichoderma lives living bacteria count>=10 of Trichoderma in original spore powder 9cfu/g.
5. Chinese medicinal materials plantation substrate according to claim 1, it is characterized in that: after step 3) adds dimethomorph mancozeb and amino acid SODm solution, in matrix, amino acid concentration is 100-200g/L, MnZn total amount is the massfraction of 20-40g/L, SODm is 2-10%.
6. an application for Chinese medicinal materials plantation substrate as claimed in claim 1, is characterized in that: directly add in decomposing agent, organic fertilizer or bacteria agent, for the cultivation of Chinese medicinal materials by described Chinese medicinal materials plantation substrate.
7. the application of Chinese medicinal materials plantation substrate according to claim 6, is characterized in that: described Chinese medicinal materials is the orchid family Herba Anoectochili roxburghii, Herba Dendrobii, Liliaceae Paris polyphylla, lily, Radix Apioris Fortunei (Radix Lespedezae Buergeri) and other can with the Chinese medicinal materials of Trichoderma symbiosis.
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