CN108059554A - A kind of biology expelling parasite foliar fertilizer and preparation method thereof - Google Patents
A kind of biology expelling parasite foliar fertilizer and preparation method thereof Download PDFInfo
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- CN108059554A CN108059554A CN201610981741.1A CN201610981741A CN108059554A CN 108059554 A CN108059554 A CN 108059554A CN 201610981741 A CN201610981741 A CN 201610981741A CN 108059554 A CN108059554 A CN 108059554A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C9/00—Fertilisers containing urea or urea compounds
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/50—Treatments combining two or more different biological or biochemical treatments, e.g. anaerobic and aerobic treatment or vermicomposting and aerobic treatment
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The present invention relates to a kind of biological expelling parasite foliar fertilizers and preparation method thereof, and raw material includes following weight parts component：Compound 75 85 parts of seaweed bio-fungus liquid, 4 10 parts of horseradish leaf juice, 48 parts of capsicum water, 37 parts of molasses, 4 parts of amino acid/11,0.1 1 parts of trace element.Biological expelling parasite foliar fertilizer made of the present invention has the food cauline leaf insects such as stinkbug and significantly drives and preventive effect；There is promotion plant strain growth simultaneously, improve photosynthesis, the effect for improving product quality, increasing crop yield.The present invention is using the water of biological source as repellent materials, the compound seaweed microbial bacterial agent rich in seaweed elite, amino acid and appropriate trace element, it then follows the research and development theory of " ground pest is based on driveing " meets the requirement of ecological protection.Simple production process is convenient, and Product Green environmental protection, expelling parasite, effect of increasing production are apparent.
Description
Technical field
The invention belongs to microbial bacterial agent technical field scopes, and in particular to a kind of biology expelling parasite foliar fertilizer and its preparation side
Method.
Background technology
With the increase of world population, especially developing country, grain and agricultural product are needed increasingly to increase, chemistry
Pesticide is still the means the most cost-effective for ensureing cereal crops volume increase, stablizing.Pesticide is a kind of toxic chemical, pesticide
With a long history in use agriculturally, it can ensure the growth for promoting plant and crops, can with desinsection sterilization weeding,
Agricultural product receive after accumulating and processing etc. during also function to important function, even if pesticide is write in the survival and development history of the mankind
Dense one has been descended, but the harmfulness of pesticide cannot be ignored, pesticide has carcinogenic teratogenesis in vivo, influences nerve etc.
Toxic action, and destroy ecological environment.
Pesticide has given birth to serious harm to human body and other biological productions, and threat is constituted to bio-diversity.It is planted to prevent
Object pest and disease damage, the whole world have more than 600 ten thousand tons of chemical pesticides to be sprayed in natural environment every year, actual to play only the 1% of efficiency,
Remaining and 99% all dissipate among soil, air and water body.
In recent years, since pesticide variety is more and more, some peasants not attention method and approach when in use cause tight
The ecological pollution of weight.It with chemical pesticide large area, uses in all directions, entire Agro-ecological System deteriorates increasingly.Together
When the intoxicating phenomenon that occurs due to pesticide residue it is also commonplace.With the raising of lives of the people level, people more value
Quality of the life, also natural to select those delicate mouthfeels, safe pure green vegetables, these all cause researcher and production
Enterprise starts attention to turn to low toxicity, low-residual, the exploitation and use for being not likely to produce drug-fast biological pesticide.
Biological foliage fertilizer is the general designation for the fertilizer for providing nutriment for crop by crop leaf, and nutrient is applied
In crops blade surface, basic function is played by the absorption of blade.The major function of blade is carried out using solar energy
Photosynthesis synthesizes organic matter, and synthetic product is carried out certain biochemical transformation and is transported to other organs.High terrestrial
The blade of plant is generally made of epidermis, mesophyll, vein three parts.The epidermis of leaf is divided into the palisade cell and lower epidermis of epicuticle
Sponge cell.There is the aperture of the lens-shaped of guard cell's formation in the upper lower epidermis of blade, be known as stomata, be that carbon is same
The access of air and vapor in the gaseous metabolisms such as change, transpiration.Research shows that blade and external world's progress mass exchange are main
It is the hydrophilic aperture of stomata by blade surface and cuticula.There is one layer of cuticula outside plant leaf blade epidermal cell, by one
Kind can allow aqueous solution to penetrate into leaf with hydroxyl, carboxylated hydrophilic group.In addition the matter ectodesma of blade cell can lead to
It crosses active absorption and nutriment is transported to blade interior.
, need not be by soil since foliar fertilizer is directly to spray application on leaf, therefore there are following features：(1) fertilizer efficiency
It is high.Foliar fertilizer is directly sprayed on blade face, and utilization rate is higher, it is possible to reduce the total dosage of fertilizer.(2) Nutrient Absorption is fast.It is not required to
It to be absorbed by root system, very long transportational process, the foliage dressing such as stalk transport can rapidly improve plant nutrient shape in time
Condition.(3) it is with strong points.It can spray the element that supplement lacks in time according to crop leaf nutritional deficiency feature and improve symptom.(4)
Supplement deficiency of the root to Nutrient Absorption.The plant growth later stage since root functionality fails, absorbs nutrient energy force difference, by using leaf
Leaven can improve yield, improving quality.(5) method of administration is simple and practicable, remarkable in economical benefits.
A variety of advantages of foliar fertilizer cause it to be widely used in agricultural, and kind is also more and more.In recent years,
With the production of multi-element leaf fertilizer, the update of fertilizer type, the application of foliar fertilizer has made great progress, and people start to consider
Pest-resistant anthelmintic ingredient is added in foliar fertilizer, fertilizer efficiency and drug effect are combined into one.
CN104725145A discloses a kind of organic foliar fertilizer with anthelmintic action, and composition includes biogas slurry, extract A
With plant powder B, the wherein raw material of biogas slurry include chicken manure, sheep manure, tea seed cake, pine needle, wormwood wheat stalk；Extract A
Raw material include sweetscented oleander leaf, barna leaf, setose thistle leaf, camphortree leaf, chrysanthemum cinesariaefolium, cordate houttuynia；The raw material bag of plant powder B
Include camphor tree dry hide, Chinese prickly ash, Cortex Phellodendri.Chicken manure typically contains the very copper of a large amount, mercury, chromium, cadmium, lead, arsenic etc. in raw material selected by the invention
Heavy metal also has more hormone, antibiotic residue, can cause agricultural product heavy metals exceeding standard, hidden danger is brought to food security；
On fermentation time, foregoing invention fermentation time needs at least six months, and the cycle is longer；In addition, though the expelling parasite in foregoing invention
What ingredient all selected is plant source, but sweetscented oleander leaf, chrysanthemum cinesariaefolium etc. belong to poisonous plant, except there is certain danger to human body
Evil is outer, can also kill insect (including beneficial insect and harmful insect), can have a negative impact to the ecosystem.
CN104130068A discloses a kind of composite multi-functional biological foliage fertilizer, is made of the raw material of following parts by weight meter
Uniformly it is mixed to prepare：Trichoderma Viride liquid 30-45, Rhizopus stolonifer zymotic fluid 30-45, Boratex 1-3, water-soluble chitosan
0.5-1.5, potassium fulvate 1-2, composite aminophenol powder 5-10.Rhizopus stolonifer is a kind of pathogenic bacteria in raw material selected by the invention, energy
A variety of storage grains such as grain, fruit is enough caused to go mouldy, and the invention does not contain the ingredient of expelling parasite.
The content of the invention
Therefore, it is solution problem above, present invention offer one kind is of low cost, is formulated reasonable, short preparation period, user
Just biological expelling parasite foliar fertilizer that, performance is stable, biological effectiveness is high and preparation method thereof uses complex micro organism fungicide to carry
Body, the horseradish leaf juice and capsicum water for adding plant source are pest repellant, and a kind of two-in-one biological leaf for possessing fertilizer efficiency and drug effect is made
Leaven meets the development trend of water-fertilizer integral and biological pesticide.
The technical solution adopted by the present invention is as follows：
A kind of biology expelling parasite foliar fertilizer, raw material include following weight parts component：
Compound 75-85 parts of seaweed bio-fungus liquid, 4-10 parts of horseradish leaf juice, 4-8 parts of capsicum water, 3-7 parts of molasses, amino acid/11-
4 parts, 0.1-1 parts of trace element.
Preferably, biological expelling parasite leaf fertilizer raw material includes following weight parts component：
Compound 82 parts of seaweed bio-fungus liquid, 6 parts of horseradish leaf juice, 5 parts of capsicum water, 5 parts of molasses .9 parts of amino acid/11, micro member
0.1 part of element.
The compound seaweed bio-fungus liquid is mixed by aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution.
The aerobic fermentation bacterium solution, anaerobic fermentation bacterium solution press (35-45)：The ratio mixing of (55-65).
The aerobic fermentation bacterium solution be finished by streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris and film mould it is red
Saccharomycete carry out expanding respectively it is numerous after, carry out ventilation in the medium and cultivate；The anaerobic fermentation bacterium solution is Cheesecake breast bar
Anaerobic culturel is carried out in the medium after bacterium expansion is numerous to form；The medium component includes seaweed slurry, molasses, urea, low-diet salt
And water.
Streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris and film mould finish red ferment in the aerobic fermentation bacterium solution
Female bacterium weight ratio is (1.5-2.5)：(4.5-5.5)：(1.5-2.5)：(0.5-1.5).
The horseradish leaf juice is to pass through the liquid squeezed the juice by raw material of fresh horseradish leaf.
The capsicum water is to pass through the obtained liquid of boiling using capsicum annum fasciculatum as raw material, and the proportion of capsicum annum fasciculatum and water is (3.5-
7.5)：(92.5-96.5), boiling time are 0.4-2h, preferably 0.5h.
The molasses are cane molasses；The amino acid be amino acid original powder, content >=45%；The trace element is chela
State copper, Chelating state zinc, Chelating state boron are closed, its ratio be 4：15：1.
Living bacteria count is greater than or equal to 2 × 10 in the biology expelling parasite foliar fertilizer8CFU/mL。
A kind of preparation method of biology expelling parasite foliar fertilizer, comprises the following steps：
S1, fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
S2, capsicum annum fasciculatum is taken, after adding boiling boiling 0.4-2h, capsicum water is made in filtering, spare；；
S3, streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, Pichia membranaefaciens bacterium are expanded respectively
After numerous, aerobic fermentation is carried out in the culture medium starched containing seaweed in formula ratio ratio, obtains aerobic fermentation bacterium solution；
S4, by Lactobacillus casei expand it is numerous after, anaerobic fermentation is carried out in the culture medium starched containing seaweed, obtains anaerobism
Zymocyte liquid；
S5, aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are mixed in proportion, compound seaweed bio-fungus liquid is made, it is spare；
S6, by 75-85 parts of the compound seaweed bio-fungus liquid of formula ratio, 4-10 parts of horseradish leaf juice, 4-8 parts of capsicum water, molasses
3-7 parts, -4 parts of amino acid/11,0.1-1 parts of trace element be mixed and stirred for uniformly, ferment at a temperature of 25-37 DEG C 2-4 days to get
Required biological expelling parasite foliar fertilizer.
Fermentation temperature is 25-37 DEG C in step S3, S4, and fermentation time is 2-4 days.
Seaweed slurry in step S3, S4 in culture medium is through clear water soaking flushing, powder by algae such as kelp or Enteromorphas
After broken, grinding, it is delivered to pulper slurrying and obtains.
The present invention has the advantages that compared with the prior art：
(1) strain contained by the present invention is the combination of a variety of effective microbes, the training that each microorganism species pass through adaptation
It supports fermentation under base and condition of culture and obtains the preferable bacterium solution of vigor, these microorganisms are by synergistic effect, Relationship With Proliferation each other
Forming complexity, stable structure, a function widely has the flora of multiple-microorganism, can rapid decomposing organic matter, and generation
It thanks out to antioxidant, inhibits harmful microbe growth and breeding, and plant cell division, cell repair and enhancing can be promoted certainly
My regulatory function.
(2) horseradish leaf juice of the present invention is the liquid that is obtained by way of squeezing using horseradish leaf as raw material, wherein containing
There are effectively expelling parasite composition glucosinolate, glucosinolate of the invention that enzyme digestion reaction can occur after extruding is squeezed the juice, generate
The isosulfocyanate compounds such as 3- cyclobutenyls isothiocyanates, phenyl isothiocyanate and benzyl isothiocyanate, are commonly called as peppery
Root element, has unique irritation and pungent fragrant spicy.Athomin is the system that plant generates in long-term evolutionary process
The adaptation environment of row protects the Mechanism and FunctionsDNA of itself, it can resist the infringement of pathogen or insect.
(3) capsicum water of the present invention is the capsaicine extracted by way of boiling in capsicum.Capsaicine can be used for driving
Worm, desinsection.In organic production plantation, common capsicum water cooperation suds kill aphid.
(4) seaweed slurry in bacterium solution fermentation medium of the present invention contains more Sargassum protein and iodine, seaweed egg
In vain repair be completely cured, bad root, cellular damage and strong crop root etc. have fabulous effect, iodine is then naturally to kill virus
Fungicide；Seaweeds in seaweed slurry contain various plants somatomedin and mineral element, chelated metal ions and sea
Foreign bioactive substance, the body fluid for plant cell can be promoted quickly to divide, enhance metabolism, accelerating growth, improve body are exempted from
Epidemic disease promotes the cellular immunity of body, improves immunity, resistance, and cold, arid and the ability for alleviating pest and disease damage are resisted in enhancing.
(5) biological expelling parasite foliage fertilizer formulation of the present invention, is obtained by multiple a large number of experiments.It is closed by adding
Suitable horseradish leaf juice ratio, reach can expelling parasite will not have much impact again to microbial growth in fertilizer.Empirical tests,
Spray the pachyrhizus after the biological expelling parasite foliar fertilizer for preparing of the present invention, 1 it is small when to stinkbug purge ratio up to more than 90%, 3 it is small when drive away
Rate up to 100%, 120 it is small when interior no rebound phenomenon, anthelminthic effect is apparent.
(6) the biological expelling parasite foliar fertilizer that the present invention is produced is rich in multiple beneficial microorganism, amino acid, trace element and sea
Contained multiple nutritional components, are combined by biological fertilizer efficiency and drug effect in algae, have remarkable result in terms of volume increase.Through examination
It tests, is sprayed using commercially available common micro-organisms foliar fertilizer, the cowpea per mu yield commodity cowpea 1250kg of plantation；Identical agronomic conditions
The cowpea of the lower biological expelling parasite foliar fertilizer application prepared using the present invention, per mu yield commodity cowpea 1430g, volume increase 14.40%, and nothing
Insect pest occurs, and commodity rate effect of increasing production is apparent.
(7) product of the present invention is with obvious effects in terms of growth and development of plants, the generation of prevention pest and disease damage, using water wisely is promoted.
Description of the drawings
In order to make the content of the present invention more clearly understood, below according to specific embodiments of the present invention and combine
Attached drawing, the present invention is described in further detail, wherein
Fig. 1 is biological expelling parasite foliar fertilizer preparation technology flow chart.
Specific embodiment
The present invention can be embodied in many different forms, and should not be construed as limited to embodiment set forth herein.
On the contrary, provide these embodiments so that the disclosure will be thorough and complete, and the design of the present invention will be fully conveyed to
Those skilled in the art, the present invention will only be defined by the appended claims.
Because of the features such as absorption of trace elements is difficult, stability is poor, easily precipitated when being coexisted with other materials, to avoid the above problem
Occur, the present invention selects Chelating state when using trace element.Chelated microelements refer to chelating agent trace element
It wraps up, the present invention selects EDTD (C10H16N2O8) and its disodium salt (C10H14N2Na2O8) do chelating agent, gained Chelating state
Copper, Chelating state zinc, Chelating state boron are respectively EDTA- copper, EDTA- zinc and EDTA- boron.
Chelating state copper that the present invention uses, Chelating state zinc, Chelating state boron are carried by the production of Chengdu auspicious cloud Chemical Co., Ltd.
For.
Embodiment 1：
Biological expelling parasite foliar fertilizer in the present embodiment is prepared in following manner：
(1) horseradish leaf juice is prepared：Fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
(2) capsicum water is prepared：5.5 kilograms of capsicum annum fasciculatum is taken, after 94.5 kg of water is added to boil 0.4h, capsicum water is made in filtering,
It is spare；
(3) seaweed slurry is prepared：Kelp is taken after clear water soaking flushing, crushing, grinding, is delivered to pulper slurrying, is made
Seaweed slurry is obtained, it is spare.
(4) compound seaweed bio-fungus liquid is prepared：By streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, film mould
Pichia yeast carry out expanding respectively it is numerous after, be by weight proportion 1.5：4.5：2.5：1.5 mix and access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C ventilation culture 3 days, obtain aerobic fermentation bacterium solution, it is spare；By Lactobacillus casei expand it is numerous after, access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C Anaerobic culturel 3 days, obtains anaerobic fermentation bacterium solution, spare；Aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are pressed 35：65 ratio
Mixing, is made compound seaweed bio-fungus liquid, spare；
(5) mixed fermentation：By compound seaweed bio-fungus liquid, horseradish leaf juice, capsicum water, cane molasses, amino acid original powder (its
Middle amino acid content >=45%), Chelating state copper, Chelating state zinc, (its ratio be 4 for Chelating state boron：15：1) trace element presses 81：7：
4：6：1.6：0.4 ratio is mixed and stirred for uniformly, and 4 days are fermented at a temperature of 25-37 DEG C to get required biological expelling parasite blade face
Fertilizer.
Embodiment 2：
Biological expelling parasite foliar fertilizer in the present embodiment is prepared in following manner：
(1) horseradish leaf juice is prepared：Fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
(2) capsicum water is prepared：4 kilograms of capsicum annum fasciculatum is taken, after 96 kg of water is added to boil 1h, capsicum water is made in filtering, spare；
(3) algae slurry is prepared：Enteromorpha is taken to be delivered to pulper slurrying after clear water soaking flushing, crushing, grinding, is made
Seaweed slurry, it is spare.
(4) compound seaweed bio-fungus liquid is prepared：By streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, film mould
Pichia yeast carry out expanding respectively it is numerous after, be by weight proportion 2：5.5：1.5：1 mixes and accesses containing 8% seaweed slurry, 8% sugar
Honey, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt% of culture medium, 25-37 DEG C of ventilation
Culture 2 days, obtains aerobic fermentation bacterium solution, spare；By Lactobacillus casei expand it is numerous after, access contains 8% seaweed slurry, 8% sugar
Honey, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt% of culture medium, 25-37 DEG C of anaerobism
Culture 2 days, obtains anaerobic fermentation bacterium solution, spare；Aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are pressed 40：60 ratio mixing,
Compound seaweed bio-fungus liquid is made, it is spare；
(5) mixed fermentation：By compound seaweed bio-fungus liquid, horseradish leaf juice, capsicum water, cane molasses, amino acid original powder (its
Middle amino acid content >=45%), Chelating state copper, Chelating state zinc, (its ratio be 4 for Chelating state boron：15：1) trace element presses 75：
10：5：5：4：1 ratio is mixed and stirred for uniformly, and 3 days are fermented at a temperature of 25-37 DEG C to get required biological expelling parasite blade face
Fertilizer.
Embodiment 3：
Biological expelling parasite foliar fertilizer in the present embodiment is prepared in following manner：
(1) horseradish leaf juice is prepared：Fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
(2) capsicum water is prepared：7 kilograms of capsicum annum fasciculatum is taken, after 93 kg of water is added to boil 2h, capsicum water is made in filtering, spare；
(3) algae slurry is prepared：Kelp is taken to be delivered to pulper slurrying after clear water soaking flushing, crushing, grinding, is made
Seaweed slurry, it is spare.
(4) compound seaweed bio-fungus liquid is prepared：By streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, film mould
Pichia yeast carry out expanding respectively it is numerous after, be by weight proportion 2.5：5.5：1.5：0.5 mix and access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C ventilation culture 3 days, obtain aerobic fermentation bacterium solution, it is spare；By Lactobacillus casei expand it is numerous after, access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C Anaerobic culturel 3 days, obtains anaerobic fermentation bacterium solution, spare；Aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are pressed 35：65 ratio
Mixing, is made compound seaweed bio-fungus liquid, spare；
(5) mixed fermentation：By compound seaweed bio-fungus liquid, horseradish leaf juice, capsicum water, cane molasses, amino acid original powder (its
Middle amino acid content >=45%), Chelating state copper, Chelating state zinc, (its ratio be 4 for Chelating state boron：15：1) trace element presses 82：6：
4：6：1.5：0.5 ratio is mixed and stirred for uniformly, and 4 days are fermented at a temperature of 25-37 DEG C to get required biological expelling parasite blade face
Fertilizer.
Embodiment 4：
Biological expelling parasite foliar fertilizer in the present embodiment is prepared in following manner：
(1) horseradish leaf juice is prepared：Fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
(2) capsicum water is prepared：6.5 kilograms of capsicum annum fasciculatum is taken, after 93.5 kg of water is added to boil 0.5h, capsicum water is made in filtering,
It is spare；
(3) algae slurry is prepared：Enteromorpha is taken to be delivered to pulper slurrying after clear water soaking flushing, crushing, grinding, is made
Seaweed slurry, it is spare.
(4) compound seaweed bio-fungus liquid is prepared：By streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, film mould
Pichia yeast carry out expanding respectively it is numerous after, be by weight proportion 1.5：5.5：1.5：1.5 mix and access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C ventilation culture 4 days, obtain aerobic fermentation bacterium solution, it is spare；By Lactobacillus casei expand it is numerous after, access containing 8% seaweed slurry,
8% molasses, 0.1% urea, 0.1% low-diet salt and water aseptic culture medium in, inoculum concentration is the 10wt%, 25-37 of culture medium
DEG C Anaerobic culturel 3 days, obtains anaerobic fermentation bacterium solution, spare；Aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are pressed 45：55 ratio
Mixing, is made compound seaweed bio-fungus liquid, spare；
(5) mixed fermentation：By compound seaweed bio-fungus liquid, horseradish leaf juice, capsicum water, cane molasses, amino acid original powder (its
Middle amino acid content >=45%), Chelating state copper, Chelating state zinc, (its ratio be 4 for Chelating state boron：15：1) trace element presses 78：6：
8：7：2.8：0.2 ratio is mixed and stirred for uniformly, and 2 days are fermented at a temperature of 25-37 DEG C to get required biological expelling parasite blade face
Fertilizer.
Application examples 1
Biological expelling parasite foliar fertilizer prepared by embodiment 1 is carried out 1 to pachyrhizus：300 spray, and 1 reaches stinkbug purge ratio when small
More than 90%, 3 it is small when purge ratio up to 100%, 120 it is small when interior no rebound phenomenon.
Comparative example 1
Under identical agronomic conditions, commercially available common micro-organisms microbial inoculum foliar fertilizer, no anthelminthic effect are sprayed.
Application examples 2
Biological expelling parasite foliar fertilizer prepared by embodiment 2 is carried out 1 to cowpea：300 spray, and spray in every 10 days once, is produced through surveying,
1380-1460 kilograms of the cowpea per mu yield of plantation, 1430 kilograms of average yield per mu, during which no disease and pests harm generation, commodity rate 100%.
Comparative example 2
Under identical agronomic conditions, commercially available common micro-organisms microbial inoculum foliar fertilizer is sprayed, is produced through surveying, the cowpea per mu yield of plantation
1330-1470 kilograms, 1410 kilograms of average yield per mu, part cowpea finds that there are small holes caused by worms phenomenons not selling, and can sell cowpea 1250
Kilogram, commodity rate 88.65%.
The above embodiments are merely examples for clarifying the description, and is not intended to limit the embodiments.For institute
For the those of ordinary skill in category field, other various forms of variations or change can also be made on the basis of the above description
It is dynamic.There is no necessity and possibility to exhaust all the enbodiments.And the obvious variation or change thus extended out
Among moving still in the protection domain of the invention.
Claims (13)
1. a kind of biology expelling parasite foliar fertilizer, which is characterized in that its raw material includes following weight parts component：
Compound 75-85 parts of seaweed bio-fungus liquid, 4-10 parts of horseradish leaf juice, 4-8 parts of capsicum water, 3-7 parts of molasses, -4 parts of amino acid/11,
It is 0.1-1 parts micro-.
2. a kind of biological expelling parasite foliar fertilizer according to claim 1, which is characterized in that its raw material includes following weight parts group
Point：
Compound 82 parts of seaweed bio-fungus liquid, 6 parts of horseradish leaf juice, 5 parts of capsicum water, 5 parts of molasses .9 parts of amino acid/11, trace element
0.1 part.
3. a kind of biological expelling parasite foliar fertilizer according to claim 2, which is characterized in that the compound seaweed bio-fungus liquid be by
Aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution mix.
4. a kind of biological expelling parasite foliar fertilizer according to claim 3, which is characterized in that the aerobic fermentation bacterium solution, anaerobism hair
Yeast-like fungi liquid presses (35-45)：The ratio mixing of (55-65).
5. a kind of biological expelling parasite foliar fertilizer according to claim 4, which is characterized in that the aerobic fermentation bacterium solution is by thin yellow
Streptomycete, bacillus subtilis, Rhodopseudomonas palustris and Pichia membranaefaciens bacterium carry out expanding respectively it is numerous after, in the medium into
Row ventilation is cultivated；The anaerobic fermentation bacterium solution be Cheesecake lactobacillus expand it is numerous after carry out Anaerobic culturel in the medium and
Into；The medium component includes seaweed slurry, molasses, urea, low-diet salt and water.
A kind of 6. biological expelling parasite foliar fertilizer according to claim 5, which is characterized in that thin Huang chain in the aerobic fermentation bacterium solution
Mould, bacillus subtilis, Rhodopseudomonas palustris and Pichia membranaefaciens bacterium weight ratio are (1.5-2.5)：(4.5-5.5)：
(1.5-2.5)：(0.5-1.5).
7. a kind of biological expelling parasite foliar fertilizer according to claim 6, which is characterized in that the horseradish leaf juice is with fresh horseradish leaf
Through the liquid squeezing the juice for raw material.
8. a kind of biological expelling parasite foliar fertilizer according to claim 7, which is characterized in that in the biology expelling parasite foliar fertilizer effectively
Viable count is greater than or equal to 2 × 108CFU/mL。
9. a kind of biological expelling parasite foliar fertilizer according to claim 8, which is characterized in that the capsicum water is using capsicum annum fasciculatum as original
The proportion of the liquid that material is obtained by boiling, capsicum annum fasciculatum and water is (3.5-7.5)：(92.5-96.5), boiling time are 0.4-
2h。
10. a kind of biological expelling parasite foliar fertilizer according to claim 9, which is characterized in that the molasses are cane molasses；It is described
Amino acid be amino acid original powder, content >=45%；The trace element is Chelating state copper, Chelating state zinc, Chelating state boron, ratio
For 4：15：1.
11. a kind of preparation method of biology expelling parasite foliar fertilizer, which is characterized in that comprise the following steps：
S1, fresh horseradish leaf is taken, by way of extruding, squeezes horseradish leaf juice, it is spare；
S2, capsicum annum fasciculatum is taken, after adding boiling boiling 0.4-2h, capsicum water is made in filtering, spare；
S3, by streptomyces microflavus, bacillus subtilis, Rhodopseudomonas palustris, Pichia membranaefaciens bacterium carry out expanding respectively it is numerous after,
Aerobic fermentation is carried out in the culture medium starched containing seaweed in formula ratio ratio, obtains aerobic fermentation bacterium solution；
S4, by Lactobacillus casei expand it is numerous after, anaerobic fermentation is carried out in the culture medium starched containing seaweed, obtains anaerobic fermentation
Bacterium solution；
S5, aerobic fermentation bacterium solution and anaerobic fermentation bacterium solution are mixed in proportion, compound seaweed bio-fungus liquid is made, it is spare；
S6, by 75-85 parts of the compound seaweed bio-fungus liquid of formula ratio, 4-10 parts of horseradish leaf juice, 4-8 parts of capsicum water, molasses 3-7
Part, -4 parts of amino acid/11,0.1-1 parts of trace element are mixed and stirred for uniformly, 2-4 days being fermented at a temperature of 25-37 DEG C to get institute
The biological expelling parasite foliar fertilizer needed.
12. a kind of preparation method of biology expelling parasite foliar fertilizer according to claim 11, which is characterized in that the step S3,
Fermentation temperature is 25-37 DEG C in S4, and fermentation time is 2-4 days.
13. a kind of preparation method of biology expelling parasite foliar fertilizer according to claim 12, which is characterized in that the step S3,
In S4 in culture medium seaweed slurry be by algae such as kelp or Enteromorphas after clear water soaking flushing, crushing, grinding, be delivered to slurrying
Mechanism starches to obtain.
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