CN112898081A - Preparation and use method of rice rooting and seedling strengthening nutrient substance - Google Patents

Preparation and use method of rice rooting and seedling strengthening nutrient substance Download PDF

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CN112898081A
CN112898081A CN202110288617.8A CN202110288617A CN112898081A CN 112898081 A CN112898081 A CN 112898081A CN 202110288617 A CN202110288617 A CN 202110288617A CN 112898081 A CN112898081 A CN 112898081A
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molasses
seaweed
culture solution
fermentation
solution
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熊战之
赵桂东
张凯
吴险平
曹凯歌
刘伟中
陈香华
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JIANGSU XUHUAI DISTRICT HUAIYIN AGRICULTURAL SCIENCE RESEARCH INSTITUTE
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JIANGSU XUHUAI DISTRICT HUAIYIN AGRICULTURAL SCIENCE RESEARCH INSTITUTE
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F1/00Fertilisers made from animal corpses, or parts thereof
    • C05F1/005Fertilisers made from animal corpses, or parts thereof from meat-wastes or from other wastes of animal origin, e.g. skins, hair, hoofs, feathers, blood
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • A01G22/22Rice
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/40Treatment of liquids or slurries
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/60Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Soil Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Plant Pathology (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to a preparation and application method of a nutrient substance for rooting and strengthening rice seedlings, which comprises the following steps: preparing molasses culture solution; preparing seaweed residue composite matrix powder; fermenting, namely adding a microbial inoculum into the sugar liquid culture solution, adding the sugar liquid culture solution containing the microbial inoculum into the seaweed complex medium, and fermenting to obtain a fermentation primary product; distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid. The sugar solution culture solution can provide enough carbon source, the actinomycetes has degradation effect on substances which are difficult to decompose and utilize, such as hemicellulose, cellulose, lignin and the like contained in the seaweed stock solution, the produced substances are easy to be absorbed by plants, the resistance and the immunity of the plants and the animals to various diseases are enhanced, nutrient components which are easy to absorb by rice are produced after fermentation, and the residual saccharomycetes, filamentous fungi and actinomycetes can continuously play a role to synthesize active substances for promoting root system growth and cell division.

Description

Preparation and use method of rice rooting and seedling strengthening nutrient substance
Technical Field
The invention relates to the technical field of plant nutrient preparation, in particular to a preparation and application method of a nutrient for rooting and strengthening rice seedlings.
Background
The seaweed cultivation amount in China is at the beginning and the end of the world, the industrial production value of the seaweed plays an important role in marine economy, the seaweed cultivation amount contains substances such as hemicellulose, cellulose and lignin which are difficult to decompose and utilize, and also contains a large amount of nutrient substances such as protein, the content of arginine, lysine, methionine and cysteine in the composition of the protein is high, and the seaweed cultivation amount also contains a part of trace elements, particularly abundant iodine. At present, most seaweed is used as a fertilizer after being simply retted by seaweed processing enterprises, so that the seaweed has certain effects of nutrition, disease resistance and yield increase on crops, but the utilization rate is not high, and high-value utilization research is urgently needed.
Molasses is a byproduct of sugar industry, has different compositions due to different sugar-making raw materials and processing conditions, mainly contains a large amount of fermentable sugar (mainly sucrose), is a good fermentation raw material, can be used as a substrate or a base material of fermentation products such as yeast, monosodium glutamate, organic acid and the like, and needs to be supplemented with various nutrient salts in the fermentation process.
Disclosure of Invention
In order to overcome the defects, the invention aims to provide a preparation method and a use method of a nutrient substance for rooting and strengthening rice seedlings.
The technical scheme adopted by the invention for solving the technical problems is as follows: a preparation method of a nutrient substance for rooting and strengthening rice seedlings comprises the following steps:
D1. preparing molasses culture solution;
D2. preparing a seaweed complex base, wherein the seaweed stock solution, bran and a phosphorus source are mixed according to a mass ratio of 100: mixing at a ratio of 10-15:5-10, heating to 100 deg.C, sterilizing, and soaking for 48 hr to obtain seaweed compound base;
D3. fermenting, namely adding a microbial inoculum into a sugar liquid culture solution, wherein the inoculation amount of the microbial inoculum is 1%, the microbial inoculum comprises saccharomycetes, filamentous fungi and anti-microbial fungi, and the ratio of the microbial inoculum is 1: 0.5-1: 0.5-1, uniformly mixing, adding a sugar liquid culture solution containing the microbial inoculum into the seaweed complex base while stirring, wherein the mass ratio of the sugar liquid culture solution to the seaweed complex base is 40-60:100, uniformly mixing, performing composting fermentation, monitoring the temperature, and completing the fermentation when the temperature tends to be stable to obtain a fermentation primary product;
D4. distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid.
Specifically, the preparation method of the molasses culture solution comprises the following steps:
D11. diluting and acidifying molasses, wherein the brix of the molasses is 80-90Bx, and the sugar content is more than 50%, so that diluted molasses is firstly diluted, diluted sugar solution I is obtained when the brix of the molasses is 40-50Bx by adding water, acid is added for acidification at the moment, after uniform mixing, the propagation of mixed bacteria is prevented, the precipitation of ash and colloidal substances in the molasses is accelerated, and the acidity of the diluted sugar solution is adjusted;
D12. sterilizing molasses, introducing steam into the diluted sugar solution II, heating to 80-90 deg.C, maintaining for 1 hr, and sterilizing;
D13. clarifying molasses, introducing compressed air for 1 hr, standing for clarifying for 1 hr, taking out supernatant as diluted sugar solution II, and removing SO by ventilation2Or NO2And the like, as well as volatile acids and other volatile substances; on the other hand, the oxygen content in the sugar solution can be increased, and the dissolved oxygen coefficient of the sugar solution is improved, so that the proliferation of yeast is facilitated;
D14. adding water, diluting to a concentration of 20-30Bx, and adjusting pH to 4.0-4.5 to obtain molasses culture solution.
Specifically, concentrated sulfuric acid is used for acidification, the adding amount of the concentrated sulfuric acid is 0.2-0.3% of the volume of the dilute sugar solution, and a mixer is used for uniformly mixing.
Specifically, the phosphorus source adopts feather or animal hair.
A method for using the nutritive substance for rooting and strengthening the seedlings of paddy rice includes such steps as applying 30-50g of fermented liquid to one mu of paddy field, diluting by 800 times, spraying it on the leaves of paddy rice, and transplanting.
The invention has the beneficial effects that: the sugar solution culture solution can provide enough carbon source, is very suitable for the fermentation of yeast, and the amino acid, the carbohydrate and other organic matters generate fermentation force, the addition of the molasses is very beneficial to the growth of the yeast, and can provide enough carbon source, the yeast can also promote the growth of actinomycetes and the like, and the actinomycetes have degradation effect on substances which are difficult to decompose and utilize, such as hemicellulose, cellulose, lignin and the like, contained in the seaweed stock solution, the produced substances are easy to be absorbed by plants, and the resistance and the immunity of animals and plants to various diseases are enhanced; the seaweed stock solution also contains nutrient substances, such as protein, the content of arginine, lysine, methionine and cysteine in the protein is high, a part of trace elements, particularly iodine, are rich, protein and partial nutrient salt are provided, filamentous fungi can coexist with other microorganisms, particularly esters are generated beneficially, the alcohol generating capacity is high, maggots and other insect pests can be prevented, the stink can be eliminated, bran can increase a part of nitrogen source, the propagation of microbial inoculum is facilitated, some phosphorus sources are added, the mutual supplement and promotion effects are achieved, and easily-absorbed nutrient components of rice are generated after fermentation;
the residual saccharomycetes, filamentous fungi and actinomycetes can continuously play a role, amino acid, saccharide and other organic matters in the rice field of the saccharomycetes generate fermentation capacity, active substances for promoting root growth and cell division are synthesized, the growth of the actinomycetes and the filamentous fungi is promoted, the generated unicellular protein is indispensable nutrient for animals, the combined production of aquatic products such as fishes and shrimps is facilitated, the actinomycetes can obtain amino acid, nitrogen and the like from photosynthetic bacteria, various antibiotics, vitamins and enzymes are generated, and pathogenic bacteria can be directly inhibited.
Detailed Description
The present invention will now be described in further detail.
Example 1
A preparation method of a nutrient substance for rooting and strengthening rice seedlings comprises the following steps:
D1. preparing molasses culture solution, and treating molasses by adopting the following method:
D11. diluting and acidifying molasses, wherein the brix of the molasses is 80-90Bx, and the sugar content is more than 50%, so that diluting is carried out firstly, when the brix of the molasses is diluted to 40-50Bx by adding water, a first dilute sugar solution is obtained, acid is added for acidification at the moment, after uniform mixing, the propagation of mixed bacteria is prevented, the precipitation of ash and colloidal substances in the molasses is accelerated, and meanwhile, the acidity of the dilute sugar solution is adjusted, and a second dilute sugar solution is obtained;
D12. sterilizing the molasses, namely introducing steam into the diluted sugar solution II, heating to 80 ℃, maintaining for 1 hour, and sterilizing;
D13. clarifying molasses, introducing compressed air for 1 hr, standing for clarifying for 1 hr, taking out supernatant as diluted sugar solution III, and ventilating to remove SO2Or NO2And the like, as well as volatile acids and other volatile substances; on the other hand, the oxygen content in the sugar solution can be increased, and the dissolved oxygen coefficient of the sugar solution is improved, so that the proliferation of yeast is facilitated;
D14. adding water, diluting to a volume of 20-30Bx, and adjusting pH to 4.0 to obtain molasses culture solution.
Specifically, concentrated sulfuric acid is used for acidification, the adding amount of the concentrated sulfuric acid is 0.2-0.3% of the volume of the dilute sugar solution, and a mixer is used for uniformly mixing.
D2. Preparing a seaweed complex base, wherein the seaweed stock solution, bran and a phosphorus source are mixed according to a mass ratio of 100: mixing at a ratio of 10:10, heating to 100 ℃, sterilizing, disinfecting, and soaking for 48h to obtain a seaweed composite base;
D3. fermenting, namely adding a microbial inoculum into a sugar liquid culture solution, wherein the inoculation amount of the microbial inoculum is 1%, the microbial inoculum comprises saccharomycetes, filamentous fungi and anti-microbial fungi, and the ratio of the microbial inoculum is 1: 0.5: 0.5, uniformly mixing, adding a sugar solution culture solution containing the microbial inoculum into the seaweed complex base while stirring, wherein the mass ratio of the sugar solution culture solution to the seaweed complex base is 40:100, uniformly mixing, fermenting, monitoring the temperature, and finishing fermentation when the temperature tends to be stable to obtain a fermentation primary product;
D4. distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid.
Specifically, in the compost fermentation process, the water content is monitored, the water content is controlled to be 50% -55%, and rapid fermentation is promoted.
Specifically, the phosphorus source adopts feather or animal hair.
A method for using the nutritive substance to take root and strengthen seedlings of paddy rice includes such steps as diluting the fermented liquid by 1000 times, spraying it on the surface of the newly transplanted rice field, and spraying it once every 15 days.
Example 2
A preparation method of a nutrient substance for rooting and strengthening rice seedlings comprises the following steps:
D1. preparing molasses culture solution, and treating molasses by adopting the following method:
D11. diluting and acidifying molasses, wherein the brix of the molasses is 80-90Bx, and the sugar content is more than 50%, so that diluting is carried out firstly, when the brix of the molasses is diluted to 40-50Bx by adding water, a first dilute sugar solution is obtained, acid is added for acidification at the moment, after uniform mixing, the propagation of mixed bacteria is prevented, the precipitation of ash and colloidal substances in the molasses is accelerated, and meanwhile, the acidity of the dilute sugar solution is adjusted, and a second dilute sugar solution is obtained;
D12. sterilizing molasses, namely introducing steam into the diluted sugar solution II, heating to 85 ℃, maintaining for 1 hour, and sterilizing;
D13. clarifying molasses, introducing compressed air for 1 hr, standing for clarifying for 1 hr, taking out supernatant as diluted sugar solution III, and ventilating to remove SO2Or NO2And the like, as well as volatile acids and other volatile substances; on the other hand, the oxygen content in the sugar solution can be increased, and the dissolved oxygen coefficient of the sugar solution is improved, so that the proliferation of yeast is facilitated;
D14. adding water, diluting to a concentration of 20-30Bx, and adjusting pH to pH4.5 to obtain molasses culture solution.
Specifically, concentrated sulfuric acid is used for acidification, the adding amount of the concentrated sulfuric acid is 0.25 percent of the volume of the dilute sugar solution, and a mixer is used for mixing uniformly.
D2. Preparing a seaweed complex base, wherein the seaweed stock solution, bran and a phosphorus source are mixed according to a mass ratio of 100: mixing at a ratio of 13:5, heating to 100 deg.C, sterilizing, and soaking for 48 hr to obtain seaweed complex base;
D3. fermenting, namely adding a microbial inoculum into a sugar liquid culture solution, wherein the inoculation amount of the microbial inoculum is 1%, the microbial inoculum comprises saccharomycetes, filamentous fungi and anti-microbial fungi, and the ratio of the microbial inoculum is 1: 1: 0.8, uniformly mixing, adding a sugar solution culture solution containing the microbial inoculum into the seaweed complex base while stirring, wherein the mass ratio of the sugar solution culture solution to the seaweed complex base is 50:100, uniformly mixing, fermenting, monitoring the temperature, and finishing fermentation when the temperature tends to be stable to obtain a fermentation primary product;
D4. distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid.
Specifically, in the compost fermentation process, the water content is monitored, the water content is controlled to be 50% -55%, and rapid fermentation is promoted.
Specifically, the phosphorus source adopts feather or animal hair.
A method for using the nutritive substance to take root and strengthen seedlings of paddy rice includes such steps as diluting the fermented liquid by 900 times in the amount of 40g per mu of paddy field, spraying it on the surface of paddy field, and spraying it once every 15 days.
Example 3
A preparation method of a nutrient substance for rooting and strengthening rice seedlings comprises the following steps:
D1. preparing molasses culture solution, and treating molasses by adopting the following method:
D11. diluting and acidifying molasses, wherein the brix of the molasses is 80-90Bx, and the sugar content is more than 50%, so that diluting is carried out firstly, when the brix of the molasses is diluted to 40-50Bx by adding water, a first dilute sugar solution is obtained, acid is added for acidification at the moment, after uniform mixing, the propagation of mixed bacteria is prevented, the precipitation of ash and colloidal substances in the molasses is accelerated, and meanwhile, the acidity of the dilute sugar solution is adjusted, and a second dilute sugar solution is obtained;
D12. sterilizing molasses, namely introducing steam into the diluted sugar solution II, heating to 90 ℃, maintaining for 1 hour, and sterilizing;
D13. clarifying molasses, introducing compressed air for 1 hr, standing for clarifying for 1 hr, taking out supernatant as diluted sugar solution III, and ventilating to remove SO2Or NO2And the like, as well as volatile acids and other volatile substances; on the other hand, the oxygen content in the sugar solution can be increased, and the dissolved oxygen coefficient of the sugar solution is improved, so that the proliferation of yeast is facilitated;
D14. adding water, diluting to a concentration of 20-30Bx, and adjusting pH to 5 to obtain molasses culture solution.
Specifically, concentrated sulfuric acid is used for acidification, the adding amount of the concentrated sulfuric acid is 0.3 percent of the volume of the dilute sugar solution, and a mixer is used for mixing uniformly.
D2. Preparing a seaweed complex base, wherein the seaweed stock solution, bran and a phosphorus source are mixed according to a mass ratio of 100: mixing at a ratio of 15:8, heating to 100 deg.C, sterilizing, and soaking for 48 hr to obtain seaweed complex base;
D3. fermenting, namely adding a microbial inoculum into a sugar liquid culture solution, wherein the inoculation amount of the microbial inoculum is 1%, the microbial inoculum comprises saccharomycetes, filamentous fungi and anti-microbial fungi, and the ratio of the microbial inoculum is 1: 0.8: 1, uniformly mixing, adding a sugar solution culture solution containing a microbial inoculum into a seaweed complex base while stirring, wherein the mass ratio of the sugar solution culture solution to the seaweed complex base is 60:100, uniformly mixing, fermenting, monitoring the temperature, and finishing fermentation when the temperature tends to be stable to obtain a fermentation primary product;
D4. distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid.
Specifically, in the compost fermentation process, the water content is monitored, the water content is controlled to be 50% -55%, and rapid fermentation is promoted.
Specifically, the phosphorus source adopts feather or animal hair.
A method for using the nutritive substance to take root and strengthen seedlings of paddy rice includes such steps as diluting the fermented liquid by 800 times in an amount of 50g per mu of paddy field, spraying it on the surface of the paddy field, and spraying it once every 15 days.
In comparison of experiments, finished fermentation liquor obtained in the examples 1, 2 and 3 is taken and applied according to a mode, compared with a non-applied one-mu rice field, the rice field adopts the same rice seedling and adopts a mechanical transplanting mode to transplant rice, and the results show that: the finished fertilizer prepared by the embodiment has the advantages of short and strong seedling plants, high chlorophyll content, deep leaf color, thick and strong stem bases, more roots, uniform and regular growth of seedlings and high granulation rate.
Figure BDA0002981485710000081
Figure BDA0002981485710000091
Figure BDA0002981485710000092
The present invention is not limited to the above embodiments, and any structural changes made under the teaching of the present invention shall fall within the protection scope of the present invention, which is similar or similar to the technical solutions of the present invention.
The techniques, shapes, and configurations not described in detail in the present invention are all known techniques.

Claims (6)

1. A preparation method of a nutrient substance for rooting and strengthening rice seedlings is characterized by comprising the following steps: the method comprises the following steps:
D1. preparing molasses culture solution;
D2. preparing a seaweed complex base, wherein the seaweed stock solution, bran and a phosphorus source are mixed according to a mass ratio of 100: mixing at a ratio of 10-15:5-10 to obtain seaweed complex group;
D3. fermenting, namely adding a microbial inoculum into a sugar liquid culture solution, wherein the inoculation amount of the microbial inoculum is 1%, the microbial inoculum comprises saccharomycetes, filamentous fungi and anti-microbial fungi, and the ratio of the microbial inoculum is 1: 0.5-1: 0.5-1, uniformly mixing, adding a sugar liquid culture solution containing the microbial inoculum into the seaweed residue seaweed compound medium, stirring while adding, wherein the mass ratio of the sugar liquid culture solution to the seaweed residue seaweed compound medium is 40-60:100, uniformly mixing, fermenting, monitoring the temperature, and finishing fermentation when the temperature tends to be stable to obtain a fermentation primary product;
D4. distilling to remove alcohol, distilling the primary fermentation product, removing alcohol, and filtering the residual mixture containing the strain to obtain the final fermentation liquid.
2. The method for preparing the nutrient substances for rooting and strengthening the seedlings of the rice as claimed in claim 1, wherein the nutrient substances comprise the following components: the preparation method of the molasses culture solution comprises the following steps:
D11. diluting and acidifying molasses, wherein the brix of the molasses is 80-90Bx, and the sugar content is more than 50%, so diluting the molasses firstly, adding water to dilute the molasses to 40-50Bx to obtain a first dilute sugar solution, adding acid to acidify the first dilute sugar solution, and mixing the first dilute sugar solution and the second dilute sugar solution uniformly to obtain a second dilute sugar solution;
D12. sterilizing molasses, introducing steam into the diluted sugar solution II, heating to 80-90 deg.C, maintaining for 1 hr, and sterilizing;
D13. clarifying the molasses, introducing compressed air for 1 hour, standing for clarification for 1 hour, and taking out supernatant as dilute sugar solution III;
D14. adding water, diluting to a concentration of 20-30Bx, and adjusting pH to 4.0-4.5 to obtain molasses culture solution.
3. The method for preparing the nutrient substances for rooting and strengthening the seedlings of the rice as claimed in claim 2, wherein the nutrient substances comprise the following components: the acidification in the molasses culture solution treatment mode adopts concentrated sulfuric acid, the adding amount of the concentrated sulfuric acid is 0.2-0.3% of the volume of the dilute sugar solution, and a mixer is used for uniformly mixing.
4. The method for preparing the nutrient substances for rooting and strengthening the seedlings of the rice as claimed in claim 1, wherein the nutrient substances comprise the following components: the processing mode of the seaweed complex base is as follows: heating to 100 deg.C, sterilizing, and soaking for 48 h.
5. The method for preparing the nutrient substances for rooting and strengthening the seedlings of the rice as claimed in claim 1, wherein the nutrient substances comprise the following components: the phosphorus source adopts feather or animal hair.
6. A method for using nutrient substances for rooting and strengthening rice seedlings is characterized by comprising the following steps: the fermentation broth obtained from the claims 1-5 is diluted by 800-fold and sprayed on the leaf surface according to the amount of 30-50g per mu of rice field fertilizer.
CN202110288617.8A 2021-03-18 2021-03-18 Preparation and use method of rice rooting and seedling strengthening nutrient substance Pending CN112898081A (en)

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