CN114702357B - High-activity compound microbial fertilizer and preparation method thereof - Google Patents

High-activity compound microbial fertilizer and preparation method thereof Download PDF

Info

Publication number
CN114702357B
CN114702357B CN202210036953.8A CN202210036953A CN114702357B CN 114702357 B CN114702357 B CN 114702357B CN 202210036953 A CN202210036953 A CN 202210036953A CN 114702357 B CN114702357 B CN 114702357B
Authority
CN
China
Prior art keywords
parts
corn steep
steep liquor
weight
microbial fertilizer
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202210036953.8A
Other languages
Chinese (zh)
Other versions
CN114702357A (en
Inventor
杨春文
王程璐
任晓燕
安仲财
孙娜
马龙昆
张志勇
晋晓璐
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shandong Lvbang Biotechnology Co ltd
Original Assignee
Shandong Lvbang Biotechnology Co ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shandong Lvbang Biotechnology Co ltd filed Critical Shandong Lvbang Biotechnology Co ltd
Priority to CN202210036953.8A priority Critical patent/CN114702357B/en
Publication of CN114702357A publication Critical patent/CN114702357A/en
Application granted granted Critical
Publication of CN114702357B publication Critical patent/CN114702357B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05B—PHOSPHATIC FERTILISERS
    • C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C3/00—Fertilisers containing other salts of ammonia or ammonia itself, e.g. gas liquor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C5/00—Fertilisers containing other nitrates
    • C05C5/04—Fertilisers containing other nitrates containing calcium nitrate
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05C—NITROGENOUS FERTILISERS
    • C05C9/00—Fertilisers containing urea or urea compounds
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D5/00—Fertilisers containing magnesium
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00—Other inorganic fertilisers
    • C05D9/02—Other inorganic fertilisers containing trace elements
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F9/00—Fertilisers from household or town refuse
    • C05F9/04—Biological compost
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Fertilizers (AREA)

Abstract

The invention provides a high-activity compound microbial fertilizer which is prepared from the following raw materials: 5 to 20 weight parts of corn steep liquor fermentation liquor; 30-60 parts of corn steep liquor; 10-30 parts of molasses; 10-30 parts of mineral elements; 4-10 parts of calcium sugar alcohol; 0.5 to 5 weight portions of microelements. Compared with the prior art, the high-activity compound microbial fertilizer provided by the invention adopts components with specific content, realizes better interaction, has comprehensive nutrition, high colony number, high activity and long shelf life, can effectively solve the problems of soil hardening and low phosphorus and potassium absorption rate caused by excessive application of fertilizer, improves the fertilizer utilization rate, and obviously improves the product yield.

Description

High-activity compound microbial fertilizer and preparation method thereof
Technical Field
The invention relates to the technical field of microbial fertilizer preparation, in particular to a high-activity compound microbial fertilizer and a preparation method thereof.
Background
Corn steep liquor (corn steep liquor) is a byproduct of the production of corn starch using a wet process. When the corn starch is produced, the purified corn is soaked in water and dehydrated, and then soaked in 0.30% -0.5% sulfurous acid solution for 60-70 hours, wherein the soaking solution is corn slurry, and corn soluble nutrient substances such as soluble protein, free fatty acid, soluble sugar, vitamin and the like enter the corn soaking solution in the soaking process, so that the corn slurry is rich in nutrient substances. The corn steep liquor contains 24.7% of organic matters, 6.6% of total nutrition and 1.17g/L of sulfur, and is a natural microbial fermentation medium.
The compound microbial fertilizer is a living microbial fertilizer which is formed by compounding specific microorganisms and nutrient substances, and can not only provide or improve plant nutrition, but also increase the yield of agricultural products and improve the quality of the agricultural products. The compound microbial fertilizer plays roles by virtue of two parts of microorganisms and mineral elements, and the microbial effect mainly depends on thalli and metabolites; the microorganisms contained in the compound microbial fertilizer are mainly functional microorganisms including lactobacillus, saccharomycetes, bacillus, mould and the like, and mainly play roles in improving soil, dissolving phosphorus, dissolving potassium and the like, and the life activities of the microorganisms increase the supply amount of nutrient elements or improve the granular structure of the soil, so that the nutrition level of plants is improved, and the yield and quality are promoted; the plant nutrient absorption effect can be promoted by the stimulation of the plant by the metabolic substances generated by the microorganism, such as hormone substances, or the pathogenic effect of certain pathogenic microorganisms can be antagonized, so that the plant diseases and insect pests are relieved, and the crop yield is increased.
Currently, researches on producing organic fertilizer or liquid fertilizer by using corn steep liquor are carried out, for example, patent CN109305831A is used for producing biological organic fertilizer by using corn steep liquor and lysine fermentation waste liquid, patent CN111606756A is used for preparing biological composite liquid fertilizer by using corn steep liquor, and patent CN107353063A is used for producing liquid fertilizer by using corn steep liquor and thiourea slag. However, these liquid fertilizers or compound microbial fertilizers, especially liquid compound microbial fertilizers, do not fundamentally solve the problems of short shelf life and low bacterial load, and the fertilizer efficiency is reduced after long-term storage, so that a high-activity compound microbial fertilizer is needed to solve the technical problems.
Disclosure of Invention
In view of the above, the invention aims to provide the high-activity compound microbial fertilizer and the preparation method thereof, and the high-activity compound microbial fertilizer provided by the invention has the advantages of comprehensive nutrition, high colony number, high activity and long shelf life, can effectively solve the problems of soil hardening and low phosphorus and potassium absorption rate caused by excessive application of chemical fertilizer, improves the fertilizer utilization rate, and obviously improves the product yield.
The invention provides a high-activity compound microbial fertilizer which is prepared from the following raw materials:
5 to 20 weight parts of corn steep liquor fermentation liquor;
30-60 parts of corn steep liquor;
10-30 parts of molasses;
10-30 parts of mineral elements;
4-10 parts of calcium sugar alcohol;
0.5 to 5 weight portions of microelements.
Preferably, the corn steep liquor is prepared by fermenting the following raw materials by fermentation strains:
30-60 parts of corn steep liquor;
5-20 parts of corn starch;
0.5 to 3 parts by weight of peptone;
0.1 to 1 weight part of ammonium chloride;
0.1 to 1 weight part of magnesium sulfate;
0.05 to 1 part by weight of monopotassium phosphate;
0.05 to 1 part by weight of dipotassium hydrogen phosphate.
Preferably, the fermentation strain is autonomously screened and separated bacillus megatherium, and the preservation number is CGMCC No.24159.
Preferably, the preparation method of the corn steep liquor fermentation liquor specifically comprises the following steps:
a) Inoculating bacillus megatherium with the preservation number of CGMCC No.24159 into an LB liquid culture medium, and performing shake culture for 24-48 hours at the temperature of 35-37 ℃ to obtain bacillus megatherium seed liquid with the preservation number of CGMCC No.24159;
b) Placing corn steep liquor, corn starch, peptone, ammonium chloride, magnesium sulfate, monopotassium phosphate and dipotassium phosphate into a fermentation tank, and regulating the pH value to 6.8-7.0 by using 30-50 wt% alkali solution; maintaining the tank pressure at 0.08-0.15 MPa, after the tank pressure is eliminated for 25-35 min, cooling to 36-38 ℃, inoculating bacillus megatherium seed liquid with the preservation number of CGMCC No.24159, controlling the air flow at 25-35L/min, and fermenting for 20-50 h at the rotating speed of 120-200 rpm at 0.04-0.06 MPa to obtain the corn steep liquor fermentation liquor.
Preferably, the corn steep liquor is a supernatant of liquid corn steep liquor which is allowed to stand for 24 hours.
Preferably, the molasses is selected from one or more of beet molasses, cane molasses, grape molasses and corn molasses.
Preferably, the mineral elements consist of the following components in mass ratio of 7: (4-6): (4-6) Urea, KH 2 PO 4 And KNO 3 Composition is prepared.
Preferably, the sugar alcohol calcium is prepared from erythritol waste mother liquor, water and calcium nitrate.
Preferably, the trace elements are selected from one or more of EDTA-zinc, EDTA-boron, EDTA-copper, EDTA-molybdenum, EDTA-iron and EDTA-manganese.
The invention also provides a preparation method of the high-activity compound microbial fertilizer, which comprises the following steps:
and uniformly mixing the corn steep liquor, molasses, mineral elements, sugar alcohol calcium and trace elements to obtain the high-activity compound microbial fertilizer.
The invention provides a high-activity compound microbial fertilizer which is prepared from the following raw materials: 5 to 20 weight parts of corn steep liquor fermentation liquor; 30-60 parts of corn steep liquor; 10-30 parts of molasses; 10-30 parts of mineral elements; 4-10 parts of calcium sugar alcohol; 0.5 to 5 weight portions of microelements. Compared with the prior art, the high-activity compound microbial fertilizer provided by the invention adopts components with specific content, realizes better interaction, has comprehensive nutrition, high colony number, high activity and long shelf life, can effectively solve the problems of soil hardening and low phosphorus and potassium absorption rate caused by excessive application of fertilizer, improves the fertilizer utilization rate, and obviously improves the product yield.
In addition, the preparation method provided by the invention has the advantages of simple process, mild and easily controlled conditions, low production cost, easiness in industrial mass production and wide application prospect.
Drawings
FIG. 1 is a schematic diagram of a compound microbial fertilizer provided in example 1 of the present invention;
FIG. 2 is a graph showing the time course of the compound microbial fertilizer microorganism provided in example 1 of the present invention;
FIG. 3 shows the results of the composite microbial fertilizer effect verification-plant height measurement provided in example 1 of the present invention;
FIG. 4 shows the results of the effect verification-stem thickness measurement of the compound microorganism fertilizer provided in example 1 of the present invention;
FIG. 5 is a graph showing the yield statistics of the compound microbial fertilizer.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The invention provides a high-activity compound microbial fertilizer which is prepared from the following raw materials:
5 to 20 weight parts of corn steep liquor fermentation liquor;
30-60 parts of corn steep liquor;
10-30 parts of molasses;
10-30 parts of mineral elements;
4-10 parts of calcium sugar alcohol;
0.5 to 5 weight portions of microelements.
In the invention, the high-activity compound microbial fertilizer is prepared from raw materials including corn steep liquor, molasses, mineral elements, calcium sugar alcohol and trace elements, and is preferably prepared from corn steep liquor, molasses, mineral elements, calcium sugar alcohol and trace elements.
In the invention, the corn steep liquor is preferably prepared by fermenting the following raw materials by fermentation strains:
30-60 parts of corn steep liquor;
5-20 parts of corn starch;
0.5 to 3 parts by weight of peptone;
0.1 to 1 weight part of ammonium chloride;
0.1 to 1 weight part of magnesium sulfate;
0.05 to 1 part by weight of monopotassium phosphate;
0.05 to 1 part by weight of dipotassium hydrogen phosphate.
More preferably, the feed is prepared by fermenting the following raw materials by fermentation strains:
30-60 parts of corn steep liquor;
5-20 parts of corn starch;
0.5 to 3 parts by weight of peptone;
0.1 to 1 weight part of ammonium chloride;
0.1 to 1 weight part of magnesium sulfate;
0.05 to 1 part by weight of monopotassium phosphate;
0.05 to 1 part by weight of dipotassium hydrogen phosphate;
more preferably, the feed is prepared by fermenting the following raw materials by fermentation strains:
48-52 parts of corn steep liquor;
14-16 parts of corn starch;
0.8 to 1.2 weight parts of peptone;
0.4 to 0.6 weight parts of ammonium chloride;
0.15 to 0.25 weight part of magnesium sulfate;
0.08 to 0.12 weight parts of monopotassium phosphate;
0.08 to 0.12 weight parts of dipotassium hydrogen phosphate.
The sources of the corn steep liquor, corn starch, peptone, ammonium chloride, magnesium sulfate, monopotassium phosphate and dipotassium phosphate are not particularly limited, and commercially available products well known to those skilled in the art can be used.
In the invention, the fermentation strain is preferably autonomously screened and separated bacillus megatherium, and the preservation number is CGMCC No.24159. The invention screens out a microorganism strain for decomposing phosphorus and potassium from soil, which is named LBSW21-03, and carries out 16SrDNA sequencing, and the detected 16SrDNA sequence is subjected to BLAST comparison. The strain has the morphological characteristics of about 2mm of colony diameter, regular colony edge, off-white color, smooth surface and spore production. The bacillus megatherium (Bacillus megaterium) is identified on the molecular level and is preserved in China general microbiological culture Collection center (CGMCC) with the preservation number of 24159.
In the invention, the preparation method of the corn steep liquor fermentation liquor preferably comprises the following specific steps:
a) Inoculating bacillus megatherium with the preservation number of CGMCC No.24159 into an LB liquid culture medium, and performing shake culture for 24-48 hours at the temperature of 35-37 ℃ to obtain bacillus megatherium seed liquid with the preservation number of CGMCC No.24159;
b) Placing corn steep liquor, corn starch, peptone, ammonium chloride, magnesium sulfate, monopotassium phosphate and dipotassium phosphate into a fermentation tank, and regulating the pH value to 6.8-7.0 by using 30-50 wt% alkali solution; maintaining the tank pressure at 0.08-0.15 MPa, after the tank pressure is eliminated for 25-35 min, cooling to 36-38 ℃, inoculating bacillus megatherium seed liquid with the preservation number of CGMCC No.24159, controlling the air flow at 25-35L/min, and fermenting for 20-50 h at the rotating speed of 120-200 rpm at 0.04-0.06 MPa to obtain the corn steep liquor fermentation liquor.
Inoculating bacillus megatherium with the preservation number of CGMCC No.24159 into an LB liquid culture medium, and performing shake culture at the temperature of 35-37 ℃ for 24-48 hours to obtain bacillus megatherium seed liquid with the preservation number of CGMCC No.24159; then placing corn steep liquor, corn starch, peptone, ammonium chloride, magnesium sulfate, monopotassium phosphate and dipotassium phosphate into a fermentation tank, and regulating the pH value to 6.8-7.0 by using 30-50 wt% alkali solution; maintaining the tank pressure at 0.08-0.15 MPa, after the tank pressure is eliminated for 25-35 min, cooling to 36-38 ℃, inoculating bacillus megatherium seed liquid with the preservation number of CGMCC No.24159, controlling the air flow at 25-35L/min, and fermenting for 20-50 h at the rotating speed of 120-200 rpm at 0.04-0.06 MPa to obtain the corn steep liquor fermentation liquor. The bacillus bacterial liquid is prepared from the corn starch processing byproducts, namely the corn steep liquor, has wide sources, high organic matter content and low production cost, belongs to comprehensive utilization of resources, and reduces environmental pollution.
In the present invention, the alkali solution is preferably a sodium hydroxide solution, and the concentration is preferably 30wt% to 50wt%, more preferably 40wt%.
In the present invention, the tank pressure is preferably 0.1MPa to 0.12MPa, and preferably after being practically eliminated for 30min, the tank pressure is cooled to 37 ℃.
In the present invention, the air flow rate is preferably 30L/min, the tank pressure is preferably 0.05MPa, the rotation speed is preferably 140rpm to 160rpm, and the fermentation time is preferably 24h to 48h.
In the present invention, the high-activity composite microbial fertilizer comprises 5 to 20 parts by weight of corn steep liquor, preferably 8 to 12 parts by weight.
In the present invention, the corn steep liquor is preferably a supernatant of liquid corn steep liquor that is allowed to stand for 24 hours. In the present invention, the high activity composite microbial fertilizer comprises 30 to 60 parts by weight of corn steep liquor, preferably 45 to 50 parts by weight.
In the present invention, the molasses is preferably one or more selected from beet molasses, cane molasses, grape molasses and corn molasses, more preferably beet molasses, cane molasses, grape molasses or corn molasses. The source of the molasses is not particularly limited and commercially available products known to those skilled in the art may be used. In the present invention, the high activity compound microbial fertilizer comprises 10 to 30 parts by weight of molasses, preferably 15 to 25 parts by weight.
In the present invention, the mineral elements are preferably composed of a mass ratio of 7: (4-6): (4-6) Urea, KH 2 PO 4 And KNO 3 Composition is prepared. The invention aims at the urea and KH 2 PO 4 And KNO 3 The source of (c) is not particularly limited and commercially available products known to those skilled in the art may be used. In the invention, the high-activity compound microbial fertilizer comprises 10 to 30 weight partsThe amount of mineral elements is preferably 15 to 20 parts by weight.
In the invention, the sugar alcohol calcium is preferably prepared from erythritol waste mother liquor, water and calcium nitrate. The sugar alcohol calcium is produced by using the waste erythritol mother liquor, the erythritol is comprehensively utilized to reduce environmental pollution, and meanwhile, the produced sugar alcohol calcium has good freeness and high plant absorption and utilization rate. In the present invention, the high activity composite microbial fertilizer includes 4 to 10 parts by weight of mineral elements, preferably 5 to 8 parts by weight.
In the present invention, the trace element is preferably one or more selected from EDTA-zinc, EDTA-boron, EDTA-copper, EDTA-molybdenum, EDTA-iron and EDTA-manganese, more preferably EDTA-zinc, EDTA-boron, EDTA-molybdenum, EDTA-iron and EDTA-manganese. The source of the trace elements is not particularly limited, and commercially available products known to those skilled in the art may be used. In the present invention, the high activity composite microbial fertilizer comprises 0.5 to 5 parts by weight of trace elements, preferably 1 to 2 parts by weight.
The high-activity compound microbial fertilizer provided by the invention adopts components with specific content, realizes better interaction, and improves the nutrition level and osmotic pressure of the compound microbial fertilizer by adding corn steep liquor, molasses and mineral elements, so that the microorganism is in a eutrophic and high osmotic pressure state, and the microorganism cannot effectively germinate under the condition and is in a spore state; in addition, the final spore number of the high-activity compound microbial fertilizer provided by the invention is more than or equal to 2.0 multiplied by 10 ^8 cfu/mL. The high-activity compound microbial fertilizer provided by the invention has comprehensive nutrition, has the characteristics of promoting growth and improving the yield of agricultural products, contains various plant nutrition sources and chelated medium trace elements, promotes the growth of roots, stems and leaves of crops, and improves the yield of the agricultural products.
The invention also provides a preparation method of the high-activity compound microbial fertilizer, which comprises the following steps:
and uniformly mixing the corn steep liquor, molasses, mineral elements, sugar alcohol calcium and trace elements to obtain the high-activity compound microbial fertilizer.
In the present invention, the corn steep liquor, molasses, mineral elements, sugar calcium alkoxide and microelements are the same as those in the above technical solutions, and will not be described here again. The mixing mode is not particularly limited, and mechanical stirring or manual stirring technical schemes well known to those skilled in the art can be adopted, so that the raw materials are uniformly mixed.
The preparation method provided by the invention has the advantages of simple process, mild and easily controlled conditions, low production cost, easiness in industrial mass production and wide application prospect.
The invention provides a high-activity compound microbial fertilizer which is prepared from the following raw materials: 5 to 20 weight parts of corn steep liquor fermentation liquor; 30-60 parts of corn steep liquor; 10-30 parts of molasses; 10-30 parts of mineral elements; 4-10 parts of calcium sugar alcohol; 0.5 to 5 weight portions of microelements. Compared with the prior art, the high-activity compound microbial fertilizer provided by the invention adopts components with specific content, realizes better interaction, has comprehensive nutrition, high colony number, high activity and long shelf life, can effectively solve the problems of soil hardening and low phosphorus and potassium absorption rate caused by excessive application of fertilizer, improves the fertilizer utilization rate, and obviously improves the product yield.
In addition, the preparation method provided by the invention has the advantages of simple process, mild and easily controlled conditions, low production cost, easiness in industrial mass production and wide application prospect.
In order to further illustrate the present invention, the following examples are provided. The raw materials used in the following examples of the present invention are all commercial products;
biological preservation description:
LBSW21-03, classification naming: bacillus subtilis, deposited on the China general microbiological culture Collection center (CGMCC) at 12 months and 17 days of 2021, with the address of Hospital No. 3 of North Chen West Lu 1, korea, beijing, and the deposit number of CGMCC No.24159;
in the following examples, the bacillus seed liquid with the preservation number of CGMCC No.24159 is obtained according to the following steps: inoculating bacillus megatherium with the preservation number of CGMCC No.24159 into an LB liquid culture medium, and performing shake culture for 24 hours at the temperature of 35-37 ℃ to obtain bacillus megatherium seed liquid with the preservation number of CGMCC No.24159;
the corn steep liquor is supernatant liquid of liquid corn steep liquor which is kept stand for 24 hours, molasses is specifically cane molasses, and mineral elements are prepared from the following components in percentage by mass: 5: urea, KH 5 2 PO 4 And KNO 3 The sugar alcohol calcium is prepared from erythritol waste mother liquor, water and excessive calcium nitrate, and the microelements are EDTA-zinc, EDTA-boron, EDTA-molybdenum, EDTA-iron and EDTA-manganese.
Example 1
(1) Preparing corn steep liquor:
50 parts of corn steep liquor, 15 parts of corn starch, 1 part of peptone, 0.5 part of ammonium chloride, 0.2 part of magnesium sulfate, 0.1 part of monopotassium phosphate and 0.1 part of dipotassium phosphate are put into a 50L fermentation tank, the liquid filling volume is 30L, and the pH is adjusted to 6.8-7.0 by 40wt% of sodium hydroxide; maintaining the tank pressure at 0.10-0.12 MPa, sterilizing for 30min, cooling to 37deg.C, inoculating bacillus seed liquid with preservation number of CGMCC No.24159; air flow is 30L/min, tank pressure is 0.05MPa, rotating speed is 150rpm, and fermentation is carried out for 48 hours, thus obtaining corn steep liquor fermentation liquor; the number of effective bacterial colonies of the corn steep liquor fermentation liquor is more than or equal to 3.0X10 ^9 cfu/mL。
(2) Preparing a compound microbial fertilizer:
10 parts by weight of corn steep liquor fermentation liquor obtained in the step (1), 47 parts by weight of corn steep liquor, 20 parts by weight of molasses, 17 parts by weight of mineral elements, 5 parts by weight of calcium sugar alcohol and 1 part by weight of trace elements are mixed and stirred uniformly to obtain the compound microbial fertilizer; a schematic of the product is shown in figure 1.
The compound microbial fertilizer provided by the embodiment 1 of the invention is dark brown liquid, the pH is 4.5-5.5, the effective viable count is more than or equal to 2.0 hundred million cfu/mL, the total nutrition is more than or equal to 10%, and the organic matters are more than or equal to 20%.
Performance test:
(1) Stability test of compound microbial fertilizer:
the test starts at day 16 of 3, 2021 and ends at day 21, 6. The compound microbial fertilizer is placed at room temperature (about 21-29 ℃) and the effective bacterial colony number of the compound microbial fertilizer is detected every 5-7 days, the detection method refers to the measurement of the total number of bacterial colonies detected by GB 4789.2-2016 food microbiology, and the result is shown in figure 2; FIG. 2 is a graph showing the time course of the compound microbial fertilizer microorganism according to example 1 of the present invention, wherein the ordinate represents the colony count 10 ^7 cfu/mL。
From fig. 2, it can be seen that the effective colony number of the compound microbial fertilizer exceeds the theoretical addition value after 3 months of test, and the compound microbial fertilizer has high activity and better stability.
(2) Influence of the composite microbial fertilizer on tomato growth and development:
test site: the greenhouse of Shandong Lv bang biotechnology limited company is divided into three groups of 10 tomatoes, the composite microbial fertilizer product provided in the example 1 is diluted 100 times and root irrigated, the same dilution times of clear water and commercial liquid fertilizer are used for a control test, the relative growth amounts of plant height, stem thickness and the like are measured after fertilization for 1 week, and the results are shown in figures 3 and 4; wherein, fig. 3 is a result of the effect verification-plant height measurement of the compound microorganism fertilizer provided in the embodiment 1 of the present invention, the ordinate is plant height in cm, and fig. 4 is a result of the effect verification-stem thickness measurement of the compound microorganism fertilizer provided in the embodiment 1 of the present invention, the ordinate is plant height in mm.
As can be seen from figures 3-4, the compound microorganism fertilizer provided by the invention can promote growth, and the relative growth amount of 7 days exceeds that of the commercial fertilizer and the clean water group, so that the growth promoting effect is obvious.
The field verification test of the compound microorganism fertilizer was performed in the store town Dai Licun in borgxian county: and A, treatment: the compound microbial fertilizer is applied from 8 months to 12 months and is continuously used for 4 times, 2.5L/time, and the rest operations are the same as those of a control group. B, treatment: normally using fertilizer, applying 6 kg of water-soluble fertilizer, applying 5L of topdressing boron fertilizer per mu in the flowering period and applying 5L of calcium sugar alkoxide per mu in the swelling period. The results are shown in FIG. 5, and FIG. 5 shows the yield statistics of the compound microbial fertilizer.
As can be seen from FIG. 5, after the compound microbial fertilizer is applied, the yield of the experimental group is 643 jin/mu higher than that of the control group, and the profit is created to 2100 yuan/mu, and the compound microbial fertilizer provided by the embodiment 1 of the invention has high beneficial bacteria activity through verification, is promoted to be used as growth, and promotes the increase of both production and income of farmers.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (5)

1. The high-activity compound microbial fertilizer is prepared from the following raw materials in parts by weight:
5-20 parts of corn steep liquor fermentation liquor;
30-60 parts of corn steep liquor;
10-30 parts of molasses;
10-30 parts of mineral elements;
4-10 parts of calcium sugar alcohol;
0.5-5 parts by weight of microelements;
the corn steep liquor is prepared by fermenting the following raw materials by fermentation strains:
30-60 parts of corn steep liquor;
5-20 parts of corn starch;
0.5-3 parts by weight of peptone;
0.1 to 1 part by weight of ammonium chloride;
0.1-1 parts by weight of magnesium sulfate;
0.05-1 part by weight of monopotassium phosphate;
0.05-1 part by weight of dipotassium hydrogen phosphate;
the fermentation strain is bacillus megatherium which is independently screened and separated, and the preservation number is CGMCC No.24159;
the preparation method of the corn steep liquor fermentation liquor specifically comprises the following steps:
a) Inoculating bacillus megatherium with the preservation number of CGMCC No.24159 into an LB liquid culture medium, and performing shake cultivation for 24-48 hours at the temperature of 35-37 ℃ to obtain bacillus megatherium seed liquid with the preservation number of CGMCC No.24159;
b) Placing corn steep liquor, corn starch, peptone, ammonium chloride, magnesium sulfate, monopotassium phosphate and dipotassium phosphate into a fermentation tank, and adjusting the pH to 6.8-7.0 by using 30-50 wt% alkali solution; maintaining the tank pressure at 0.08-0.15 MPa, cooling to 36-38 ℃ after implementing sterilization for 25-35 min, inoculating bacillus megatherium seed liquid with the preservation number of CGMCC No.24159, controlling the air flow at 25-35L/min, and fermenting for 20-50 h at the speed of 120-200 rpm under the tank pressure of 0.04-0.06 MPa to obtain corn steep liquor;
the mineral elements are prepared from the following components in percentage by mass: (4-6): (4-6) urea, KH 2 PO 4 And KNO 3 Composition;
the microelements are selected from one or more of EDTA-zinc, EDTA-boron, EDTA-copper, EDTA-molybdenum, EDTA-iron and EDTA-manganese.
2. The high activity composite microbial fertilizer according to claim 1, wherein the corn steep liquor is a supernatant of liquid corn steep liquor which is allowed to stand for 24 hours.
3. The high activity complex microbial fertilizer of claim 1, wherein the molasses is selected from one or more of beet molasses, cane molasses, grape molasses and corn molasses.
4. The high activity composite microbial fertilizer according to claim 1, wherein the sugar alcohol calcium is prepared from erythritol waste mother liquor, water and calcium nitrate.
5. A method for preparing the high-activity composite microbial fertilizer according to any one of claims 1 to 4, comprising the following steps:
and uniformly mixing the corn steep liquor, molasses, mineral elements, sugar alcohol calcium and trace elements to obtain the high-activity compound microbial fertilizer.
CN202210036953.8A 2022-01-13 2022-01-13 High-activity compound microbial fertilizer and preparation method thereof Active CN114702357B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202210036953.8A CN114702357B (en) 2022-01-13 2022-01-13 High-activity compound microbial fertilizer and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202210036953.8A CN114702357B (en) 2022-01-13 2022-01-13 High-activity compound microbial fertilizer and preparation method thereof

Publications (2)

Publication Number Publication Date
CN114702357A CN114702357A (en) 2022-07-05
CN114702357B true CN114702357B (en) 2023-05-23

Family

ID=82167255

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202210036953.8A Active CN114702357B (en) 2022-01-13 2022-01-13 High-activity compound microbial fertilizer and preparation method thereof

Country Status (1)

Country Link
CN (1) CN114702357B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115259945A (en) * 2022-08-03 2022-11-01 伊犁哈萨克自治州林业科学研究院 Preparation method and application of weakly acidic full-nutritional amino acid liquid fertilizer
CN116730773A (en) * 2023-06-13 2023-09-12 齐齐哈尔大学 Application of corn steep liquor fermented organic fertilizer in improving soybean yield

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102242075B (en) * 2011-04-21 2013-08-28 马鞍山科邦生态肥有限公司 Semi-continuous fermentation method for bacillus used for microbial fertilizer
CN102911905A (en) * 2012-11-02 2013-02-06 黑龙江省麒麟工贸公司 Preparation method for microbial fertilizer fungicide
CN104232527A (en) * 2014-08-29 2014-12-24 湖北省生物农药工程研究中心 Process for preparing viable bacillus megaterium preparation
CN105439726A (en) * 2015-12-19 2016-03-30 佛山市艳晖生物科技有限公司 Bacillus megaterium bacterial fertilizer for onsite fermentation in farm and applications thereof
CN106010995B (en) * 2016-01-07 2019-12-03 扬州大学 A kind of method of bacillus megaterium preparation fermentation
CN108285397A (en) * 2017-01-10 2018-07-17 刘永先 A kind of pure grain fermenting organism organic fungi-manure and preparation method thereof
CN107312740A (en) * 2017-07-21 2017-11-03 江苏绿科生物技术有限公司 A kind of method for improving bacillus megaterium cell density
CN108017445A (en) * 2017-12-05 2018-05-11 菏泽金正大生态工程有限公司 A kind of reclamation of salinep-alkali soil type microbial bacterial agent and preparation method thereof
CN107794240B (en) * 2017-12-05 2021-02-12 金正大生态工程集团股份有限公司 Bacillus megaterium, polypeptide agricultural microbial agent thereof, and preparation method and application thereof
CN109305831A (en) * 2018-12-03 2019-02-05 寿光金远东变性淀粉有限公司 A method of biological organic fertilizer is made using corn pulp and fermentation waste liquid of lysine
CN110317753A (en) * 2019-06-27 2019-10-11 扬州大学 A kind of bacillus megaterium microbial inoculum, liquid fermentation process and its application
CN111172066B (en) * 2020-01-06 2022-07-01 河南新仰韶生物科技有限公司 Bacillus megaterium and application thereof
CN110923180A (en) * 2020-01-11 2020-03-27 济南航晨生物科技有限公司 Bacillus megaterium liquid high-density fermentation medium and supplementary culture method thereof

Also Published As

Publication number Publication date
CN114702357A (en) 2022-07-05

Similar Documents

Publication Publication Date Title
CN114702357B (en) High-activity compound microbial fertilizer and preparation method thereof
CN111362751B (en) Special compound microbial disease-resistant growth-promoting bacterial fertilizer for ginseng and preparation method thereof
CN102965319B (en) Microbial agent and bio-organic fertilizer special for winter jujube and preparation methods thereof
CN108865161B (en) Composite saline-alkali soil modifier and preparation and application thereof
CN105039210A (en) Complex lively bacterial preparation as well as water soluble fertilizer and application thereof
CN104744182A (en) Tobacco water retention biological organic fertilizer and preparation method thereof
CN101948780A (en) Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof
CN104177138B (en) Biological bacterial fertilizer prepared through solid fermentation technology and application of biological bacterial fertilizer
CN102942420B (en) Special microbial agent and bio-organic fertilizer for sweet cherry and preparation methods of special microbial agent
CN101993301B (en) Biological energizer
CN109355197B (en) Growth-promoting bacterium for promoting growth of saline-alkali soil alfalfa and microbial organic fertilizer thereof
CN101659931B (en) Antagonistic bacteria preventing and removing continuous cropping cucumber rhizoctonia rot and microbial organic fertilizer thereof
CN108383653A (en) A kind of method and bio-organic fertilizer preparing fertilizer using stalk
CN111484368A (en) Solid fermentation production method of microbial fertilizer and solid composite microbial fertilizer
CN107974423B (en) Soil biological activator and preparation method thereof
CN109097313B (en) Fermentation method and application of bacillus
CN1332911C (en) Biological composite bacterial manure for ginseng
CN113897316A (en) Bacillus licheniformis BLc06, functional melon and fruit seedling biological matrix prepared from same and application of functional melon and fruit seedling biological matrix
CN113881601A (en) In-situ efficient compound rhizobium group screening method
CN113755394A (en) Biological carbon-based microbial fertilizer containing bacillus amyloliquefaciens and application thereof
WO2022222136A1 (en) Environment-friendly seedling culturing fertilizer suitable for flowering plants, and production process therefor
CN106754449A (en) A kind of utilization cow dung or horsehit prepare technology and its application of liquid bio-bacterial manure
CN112795518A (en) Disease-resistant and quality-improving microbial agent special for tobacco and application thereof
CN112063552A (en) Microbial agent for preventing and treating asparagus wilt, preparation method and application thereof
CN111670782A (en) Composite microbial cultivation substrate and application thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
PE01 Entry into force of the registration of the contract for pledge of patent right

Denomination of invention: A highly active composite microbial fertilizer and its preparation method

Effective date of registration: 20230803

Granted publication date: 20230523

Pledgee: Boxing Shandong rural commercial bank Limited by Share Ltd.

Pledgor: Shandong Lvbang Biotechnology Co.,Ltd.

Registration number: Y2023980050869

PE01 Entry into force of the registration of the contract for pledge of patent right