CN102911905A - Preparation method for microbial fertilizer fungicide - Google Patents
Preparation method for microbial fertilizer fungicide Download PDFInfo
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- CN102911905A CN102911905A CN2012104325836A CN201210432583A CN102911905A CN 102911905 A CN102911905 A CN 102911905A CN 2012104325836 A CN2012104325836 A CN 2012104325836A CN 201210432583 A CN201210432583 A CN 201210432583A CN 102911905 A CN102911905 A CN 102911905A
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Abstract
The invention relates to a preparation method for a fungicide, in particular to a preparation method for a microbial fertilizer fungicide. The method is realized through the following steps: mixing bran, maize meal and rice bran in the proportion of 80:10:10; adding water into a mixture of corn steep liquor, urea, ammonium sulfate, monopotassium phosphate and magnesium sulfate , dissolving and mixing uniformly, then mixing mixture with the bran, the maize meal and the rice bran, steaming for one hour under the normal pressure, and cooling to 30 degree C; inoculating five bacterial strains to the solid medium; fermenting for 56 to 60 hours; and finally drying to obtain a finished product. When the fungicide prepared by the method is used to produce the microbial fertilizer, the addition volume of the fungicide is 0.1-0.2%. By using the product prepared through the method for producing the microbial fertilizer, not only can the use amount of the fertilizer be reduced, the yield of the crop be increased, the quality of the crop product be improved, the nutrition of the crop be increased and the harmful ingredient be reduced, but also plant diseases and insect pests can be resisted, the soil fertility can be improved and the environment pollution can be reduced.
Description
Technical field
The present invention relates to the making method of a kind of making method of microbial inoculum, particularly a kind of microbe fertilizer microbial inoculum.
Background technology
[0002] China is world agriculture big country, has supported the population that accounts for the world 22% with the arable land that accounts for the world 9%, obtained huge achievement in agriculture production, but huge cost has also been paid in the behind of achievement.Over nearly 30 years, China is for satisfying the grain-production needs, and its agrochemicals drop into the quite high ratio that reached.From the nineties in 20th century, China becomes chemical fertilizers production the biggest in the world and country of consumption.At present, China is one of the highest area of global chemical fertilizer Input Level.
The agricultural-food of throughout the year edible residual chemical fertilizer, can produce certain harm to human body, nitrate in the chemical fertilizer can be become nitrite by the human body bacterial reduction, and this is a kind of carcinogenic substance, and hypertension, heart trouble, diabetes and other diseases are brought out in the simultaneously throughout the year accumulation in human body of the toxin in the chemical fertilizer easily.For soil, use chemical fertilizer throughout the year, can cause that soil acidity changes, cause that hydrogen ion increases in the soil, easily cause soil acidification.Can dissolve some nutritive substances in the soil behind the soil acidification, under the effect of rainfall and irrigation, permeate recharge groundwater downwards, so that nutrient loss causes soil leanness, affect the growth of crop.Use chemical fertilizer throughout the year, also can cause soil compaction, fertility descends.For environment, chemical fertilizer also can produce soil and pollute.Make in the raw mineral materials and industrial chemicals of chemical fertilizer, contain various heavy radioactive substance and other objectionable constituent, they enter agricultural land soil with fertilising and pollute.Therefore, China from 2004 so far, central authorities' " No. 1 file " propose the requirement of the raising soil organism, and the planning of having formulated development " ecological agriculture " for years, this has stepped into the ranks of " sunrise industry " before impelling fertilizer, bio-fertilizer again to go to platform from the backstage.
Summary of the invention
The object of the invention is to that traditional fertilizer affects HUMAN HEALTH, destroy to plough for using, the deficiency of contaminate environment and the making method for the production of the microbe fertilizer microbial inoculum of bio-fertilizer that provides.
The object of the present invention is achieved like this:
1, the preparation of seed liquor
1. the preparation of blown-ball Azotobacter seed liquor
Take by weighing dipotassium hydrogen phosphate 0.8 gram, potassium primary phosphate 0.2 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, yeast extract paste 0.5 gram, N.F,USP MANNITOL 20 grams are dissolved in water and are settled to 1000ml, regulate PH to 7.0～7.2, namely get blown-ball Azotobacter seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation blown-ball Azotobacter was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the blown-ball Azotobacter seed liquor.
2. the preparation of bacillus megaterium seed liquor
Take by weighing glucose 50 grams, 20 milliliters of corn steep liquors, calcium carbonate 3 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, sodium-chlor 0.3 gram, Repone K 0.3 gram, manganous sulfate 0.05 gram, ferrous sulfate 0.05 gram, be dissolved in water and be settled to 1000ml, regulate PH to 7.0, namely get bacillus megaterium seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus megaterium was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the bacillus megaterium seed liquor.
3. the preparation of bacillus cereus seed liquor
Take by weighing Zulkovsky starch 30 grams, soybean cake powder 20 grams, dipotassium hydrogen phosphate 3 grams, MgSO
41.5 gram, CaCl
20.1 gram is dissolved in water and is settled to 1000ml, regulates PH to 7.2, namely gets bacillus cereus seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus cereus was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the bacillus cereus seed liquor.
4. the preparation of Pseudomonas chlororaphis seed liquor
Take by weighing peptone 5 gram extractum carniss 3 grams, sodium-chlor 5 grams are dissolved in water and are settled to 1000ml, regulate PH to 7.0, namely get Pseudomonas chlororaphis seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation Pseudomonas chlororaphis was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the Pseudomonas chlororaphis seed liquor.
5. the preparation of bacillus thuringiensis seed liquor
Get 25 milliliters of Zulkovsky starch 6 grams, glucose 6 grams, yeast powder 4 grams, potassium primary phosphate 1 gram, sal epsom 0.5 gram, corn steep liquor, be dissolved in water and be settled to 1000ml, regulate PH to 7.0, namely get bacillus thuringiensis seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus thuringiensis was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the bacillus thuringiensis seed liquor.
2, the preparation of microbe fertilizer microbial inoculum
Prepare first the solid fermentation substratum, with wheat bran, Semen Maydis powder, rice bran mixes in the ratio of 80:10:10, with wheat bran, the corn steep liquor of Semen Maydis powder and rice bran gross weight 2%, 2% urea, 1% ammonium sulfate, 0.1% potassium primary phosphate, 0.05% sal epsom is dissolved in water and admixes wheat bran behind the mixing, in Semen Maydis powder and the rice bran, moisture content in medium is controlled at 60～65%, mix, normal pressure steamed 1 hour, be cooled to 30 ℃, with the blown-ball Azotobacter seed liquor, the bacillus megaterium seed liquor, the bacillus cereus seed liquor, the Pseudomonas chlororaphis seed liquor, the bacillus thuringiensis seed liquor is inoculated into respectively in the above-mentioned solid medium, inoculum size be every kind of bacterial classification each 5%, mix rear tiling to the bamboo curtain splint of sterilization, 4～6 centimetres of thickness, in 30～32 ℃ of thermostat containers, fermented 56～60 hours, then be dried to moisturely below 10% under 45 ℃ of conditions, get product.
Produce microbial fertilizer with microbial inoculum of the present invention, raw material ratio is more extensive, can be mud, the agricultural crop straw of feces of livestock and poultry, changing food waste, municipal sewage plant, also can be the waste material of food processing enterprises, the waste residue in paper mill, waste residue of sugar refinery brewery etc., addition 0.1～0.2%.Not only can reduce the quality of the consumption of chemical fertilizer, the output that increases farm crop, raising crop products with products production microbial fertilizer of the present invention, increase the nutrition of farm crop, reduce objectionable constituent, can also resist disease and pest, increase soil fertility, environmental contamination reduction.
(4), specific embodiments
5 bacterial strains that the present invention adopts are all purchased in Chinese industrial microbial strains preservation administrative center, are respectively: blown-ball Azotobacter CICC20603, bacillus megaterium CICC20665, bacillus cereus CICC21687, Pseudomonas chlororaphis CICC20676, bacillus thuringiensis CICC23706.
1, the preparation of seed liquor
1. the preparation of blown-ball Azotobacter seed liquor
Take by weighing dipotassium hydrogen phosphate 0.8 gram, potassium primary phosphate 0.2 gram, sal epsom 0.2 gram, calcium sulfate 0.1 gram, yeast extract paste 0.5 gram, N.F,USP MANNITOL 20 grams are dissolved in water and are settled to 1000ml, regulate PH to 7.0, namely get blown-ball Azotobacter seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation blown-ball Azotobacter was cultivated 24 hours under rotary shaker 150rpm, 30 ℃ condition, namely got the blown-ball Azotobacter seed liquor.
2. the preparation of bacillus megaterium seed liquor
Take by weighing glucose 50 grams, 20 milliliters of corn steep liquors, calcium carbonate 3 grams, dipotassium hydrogen phosphate 1 gram, sal epsom 0.5 gram, sodium-chlor 0.3 gram, Repone K 0.3 gram, manganous sulfate 0.05 gram, ferrous sulfate 0.05 gram, be dissolved in water and be settled to 1000ml, regulate PH to 7.0, namely get bacillus megaterium seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus megaterium was cultivated 24 hours under rotary shaker 150rpm, 30 ℃ condition, namely got the bacillus megaterium seed liquor.
3. the preparation of bacillus cereus seed liquor
Take by weighing Zulkovsky starch 30 grams, soybean cake powder 20 grams, dipotassium hydrogen phosphate 3 grams, MgSO
41.5 gram, CaCl
20.1 gram is dissolved in water and is settled to 1000ml, regulates PH to 7.2, namely gets bacillus cereus seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus cereus was cultivated 24 hours under rotary shaker 150rpm, 30 ℃ condition, namely got the bacillus cereus seed liquor.
4. the preparation of Pseudomonas chlororaphis seed liquor
Take by weighing peptone 5 gram extractum carniss 3 grams, sodium-chlor 5 grams are dissolved in water and are settled to 1000ml, regulate PH to 7.0, namely get Pseudomonas chlororaphis seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation Pseudomonas chlororaphis was cultivated 24 hours under rotary shaker 150rpm, 30 ℃ condition, namely got the Pseudomonas chlororaphis seed liquor.
5. the preparation of bacillus thuringiensis seed liquor
Get 25 milliliters of Zulkovsky starch 6 grams, glucose 6 grams, yeast powder 4 grams, potassium primary phosphate 1 gram, sal epsom 0.5 gram, corn steep liquor, be dissolved in water and be settled to 1000ml, regulate PH to 7.0, namely get bacillus thuringiensis seed liquor substratum.Get the 100ml seed liquor and cultivate based in the 500ml triangular flask, 121 ℃ of sterilizations 20 minutes are cooled to 32 ℃, and the inoculation bacillus thuringiensis was cultivated 24 hours under rotary shaker 150rpm, 28～32 ℃ condition, namely got the bacillus thuringiensis seed liquor.
2, the preparation of microbe fertilizer microbial inoculum
Take by weighing wheat bran 800 grams, Semen Maydis powder 100 grams, rice bran 100 grams mix, corn steep liquor with 20 milliliters, the urea of 20 grams, the ammonium sulfate of 10 grams, the potassium primary phosphate of 1 gram, 0.5 adding water, the sal epsom of gram all admixes wheat bran after the dissolving, in Semen Maydis powder and the rice bran, moisture content in medium is controlled at 60～65%, mix, normal pressure steamed 1 hour, be cooled to 30 ℃, with the blown-ball Azotobacter seed liquor, the bacillus megaterium seed liquor, the bacillus cereus seed liquor, the Pseudomonas chlororaphis seed liquor, the bacillus thuringiensis seed liquor each 50 milliliters be inoculated into respectively in the above-mentioned solid medium, mix rear tiling to the bamboo curtain splint of sterilization, 4～6 centimetres of thickness, fermentation is 60 hours in 30 ℃ of thermostat containers, then be dried to moisturely below 10% under 45 ℃ of conditions, get product.
Claims (2)
1. the making method of a microbe fertilizer microbial inoculum, it is characterized in that: with wheat bran, Semen Maydis powder, rice bran mixes in the ratio of 80:10:10, with wheat bran, the corn steep liquor of Semen Maydis powder and rice bran gross weight 2%, 2% urea, 1% ammonium sulfate, 0.1% potassium primary phosphate, 0.05% sal epsom is dissolved in water and admixes wheat bran behind the mixing, in Semen Maydis powder and the rice bran, moisture content in medium is controlled at 60～65%, mix, normal pressure steamed 1 hour, be cooled to 30 ℃, 5 strain bacterial classification seed liquor are inoculated into respectively in the above-mentioned solid medium, inoculum size be every kind of bacterial classification each 5%, mix rear tiling to the bamboo curtain splint of sterilization, 4～6 centimetres of thickness, in 30～32 ℃ of thermostat containers, fermented 56～60 hours, then be dried to moisturely below 10% under 45 ℃ of conditions, get product.
2. the making method of a kind of microbe fertilizer microbial inoculum according to claim 1, it is characterized in that: institute's strain 5 strain bacterial classifications are respectively blown-ball Azotobacter, bacillus megaterium, bacillus cereus, Pseudomonas chlororaphis, bacillus thuringiensis.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333845A (en) * | 2013-07-19 | 2013-10-02 | 上海农乐生物制品股份有限公司 | Pseudomonas chlororaphis and fermenting cultivation method thereof |
| CN103787712A (en) * | 2014-03-11 | 2014-05-14 | 南通禾宝生物科技有限公司 | Method for preparing organic fertilizer |
| CN103952366A (en) * | 2014-05-22 | 2014-07-30 | 段占娥 | Composite biocontrol agent as well as preparation method and application thereof |
| CN104030740A (en) * | 2014-06-27 | 2014-09-10 | 柳州市汉森机械制造有限公司 | Additive of organic fertilizer for crops and preparation method of additive |
| CN114702357A (en) * | 2022-01-13 | 2022-07-05 | 山东绿邦生物科技有限公司 | High-activity composite microbial fertilizer and preparation method thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1594538A (en) * | 2004-07-08 | 2005-03-16 | 中国海洋大学 | Microbial community and its preparation method and use |
| CN1860908A (en) * | 2005-10-26 | 2006-11-15 | 孙荣高 | Method for producing thallus protein forage by using solid state fermentation of pomace and dreg or stalks with mixed aerobic bacteria liquor |
| CN101885625A (en) * | 2010-06-22 | 2010-11-17 | 武汉凯迪工程技术研究总院有限公司 | Solid fermentation method for producing microbial agent by using biomass power plant ash as carrier |
-
2012
- 2012-11-02 CN CN2012104325836A patent/CN102911905A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1594538A (en) * | 2004-07-08 | 2005-03-16 | 中国海洋大学 | Microbial community and its preparation method and use |
| CN1860908A (en) * | 2005-10-26 | 2006-11-15 | 孙荣高 | Method for producing thallus protein forage by using solid state fermentation of pomace and dreg or stalks with mixed aerobic bacteria liquor |
| CN101885625A (en) * | 2010-06-22 | 2010-11-17 | 武汉凯迪工程技术研究总院有限公司 | Solid fermentation method for producing microbial agent by using biomass power plant ash as carrier |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103333845A (en) * | 2013-07-19 | 2013-10-02 | 上海农乐生物制品股份有限公司 | Pseudomonas chlororaphis and fermenting cultivation method thereof |
| CN103787712A (en) * | 2014-03-11 | 2014-05-14 | 南通禾宝生物科技有限公司 | Method for preparing organic fertilizer |
| CN103787712B (en) * | 2014-03-11 | 2016-05-18 | 南通禾宝生物科技有限公司 | The preparation method of fertilizer |
| CN103952366A (en) * | 2014-05-22 | 2014-07-30 | 段占娥 | Composite biocontrol agent as well as preparation method and application thereof |
| CN104030740A (en) * | 2014-06-27 | 2014-09-10 | 柳州市汉森机械制造有限公司 | Additive of organic fertilizer for crops and preparation method of additive |
| CN114702357A (en) * | 2022-01-13 | 2022-07-05 | 山东绿邦生物科技有限公司 | High-activity composite microbial fertilizer and preparation method thereof |
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Application publication date: 20130206 |