CN1594538A

CN1594538A - Microbial community and its preparation method and use

Info

Publication number: CN1594538A
Application number: CNA2004100244534A
Authority: CN
Inventors: 胡国斌; 丛日山; 刘怡
Original assignee: Ocean University of China
Current assignee: Ocean University of China
Priority date: 2004-07-08
Filing date: 2004-07-08
Publication date: 2005-03-16
Anticipated expiration: 2024-07-08
Also published as: CN1266266C

Abstract

The invention discloses a microbiological bacterium and method for making same, wherein the microbiological bacterium comprises degreased saccharomycete, Trichoderma viride, high temperature bacillus, S. melanoferus, Nocardia and solid medium. The microbiological bacterium can be utilized as dejection treatment agent. Its preparation process comprises the three stage pure culture of these bacterial strains, and proportional mixing of the culture.

Description

A kind of microbiobacterial agent and its production and application

Technical field

The present invention relates to a kind of microbiobacterial agent and its production and application, more particularly, relate to a kind of by separating the refined Rockwell yeast of fat (Yaliowia lipolytica), trichoderma viride (Trichodermaviride), high temperature genus bacillus (Bacillus sp.), giving birth to microbiobacterial agent that black streptomycete (Steptomyces pigrifacienfs), five kinds of bacterial strains of Nocardia bacteria (Nocardia sp.) and solid medium form and preparation method thereof and as the application of manure treating agent.

Background technology

At present, be applied to the existing multiple patent of microbial fermentation microbial inoculum and the bibliographical information of feces of livestock and poultry harmless treatment, they can be divided into three types: a kind of is the indigenous flora fermentation method, promptly pile up feces of livestock and poultry, under aerobic condition, utilize the microbial reproduction of refuse itself to ferment, the shortcoming of this method is that fermentation time is long, and fermenting process can bring certain influence to environment; Second method is the fermentation that some microorganisms with fermentation function is applied to ight soil, but owing to do not utilize the function of thermophilic fermentation bacterial strain and deodorization fermentation strain, often causes fermentation not thoroughly or need the long period, and can back foul after the leavened prod moisture absorption; The third method is to utilize environmental friendliness microorganism species fermentation wastes, the multi-functional that this flora has fermentation, deodorizing, kills germ, but because flora is huge, the cost height, application is restricted.

Summary of the invention

The purpose of this invention is to provide a kind of microbiobacterial agent and its production and application, it can overcome

The above-mentioned shortcoming of prior art.

A kind of microbiobacterial agent, it is characterized in that forming by separating the refined Rockwell yeast of fat, trichoderma viride, high temperature genus bacillus, living black streptomycete, five kinds of bacterial strains of Nocardia bacteria and solid medium, it is 15～30: 20～40 that this five kinds of bacterial strains bacterium in every gram microbial inoculum is counted the scope ratio: 15～30: 15～30: 15～30.

The preparation method of mentioned microorganism microbial inoculum, it is characterized in that, at first will separate the refined Rockwell yeast of fat, trichoderma viride, high temperature genus bacillus, give birth to black streptomycete, Nocardia bacteria does successively respectively that primary inclined plane is cultivated, secondary liquid oscilaltion is cultivated, three grades of solid fermentations are cultivated, the bacterium number to every gram solid medium reaches 0.5～1.2 * 10 respectively ⁸Individual, 1.0～5.0 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual; Described solid fermentation is cultivated and is carried out on solid medium, before the use, regulates moisture content to 40～50%, 121～122 ℃ in the substratum, and moist heat sterilization 30～40 minutes is cooled to room temperature; At last, each bacterial strain pure growth is at room temperature counted scope than 15～30 by the bacterium of single bacterial strain: 20～40: 15～30: 15～30: 15～30 mix.

The mentioned microorganism microbial inoculum is as manure treating agent.

Advantage of the present invention is: used microorganism can be removed the stink substance in the ight soil pointedly--lipid acid and excrement albumen, taken into account the Decomposition of middle gentle hot stage simultaneously, so this microbial inoculum can organically combine deodorizing and two kinds of functions of fermentation, microbial transformation and metabolism heating functioin have been effectively utilized, realized removing fast of feces of livestock and poultry moisture content and stink, waste can be used as the use of high-quality fertilizer material simultaneously, and method is simple, cost is low, non-environmental-pollution.

Embodiment

The present invention is used separates the refined Rockwell yeast of fat, give birth to three kinds of bacterial strains such as black streptomycete, Nocardia bacteria oneself before 7 days June in 2004 in the typical culture collection center preservation of Wuhan University China, their preserving number is respectively CCTCC NO M95017, CCTCC NO AA92021, CCTCC NOM95018; Viride bacterial strain and the strain of high temperature genus bacillus public offering on market.

Embodiment 1

Separate the cultivation of the refined Rockwell yeast strain of fat: separate the refined Rockwell yeast of fat and at first support on the base, 30 ℃, make primary inclined plane and cultivate potato; After 48 hours, be inoculated into and shake in the bottle, 30 ℃, do secondary liquid oscilaltion and cultivate; After 36 hours, be 5% inoculum size, be inoculated on the solid medium, 30 ℃, do three grades and be cultured to the bacterium number and reach 0.8 * 10 with weight ratio ⁸Individual/the gram solid medium.

The cultivation of viride bacterial strain: trichoderma viride at first on the PDA substratum, 30 ℃, is made primary inclined plane and is cultivated; After 72 hours, be inoculated into and shake in the bottle, 30 ℃, do secondary liquid oscilaltion and cultivate; After 48 hours, be 5% inoculum size, be inoculated on the solid medium, 30 ℃, do three grades and be cultured to the bacterium number and reach 3.0 * 10 with weight ratio ⁸Individual/the gram solid medium.

The cultivation of high temperature genus bacillus strain: the high temperature genus bacillus at first on the meat soup protein culture medium, 30 ℃, makes primary inclined plane and cultivates; After 48 hours, be inoculated into and shake in the bottle, 30 ℃, do secondary liquid oscilaltion and cultivate; After 24 hours, be 5% inoculum size, be inoculated on the solid medium, 30 ℃, do three grades and be cultured to the bacterium number and reach 1.5 * 10 with weight ratio ⁸Individual/the gram solid medium.

Give birth to the cultivation of black streptomyces strain: living black streptomycete at first on the Gause I substratum, 30 ℃, makes primary inclined plane and cultivates; After 48 hours, be inoculated into and shake in the bottle, 30 ℃, do secondary liquid oscilaltion and cultivate; After 36 hours, be 5% inoculum size, be inoculated on the solid medium, 30 ℃, do three grades and be cultured to the bacterium number and reach 1.5 * 10 with weight ratio ⁸Individual/the gram solid medium;

The cultivation of Nocardia bacteria strain: Nocardia bacteria at first on the GY substratum, 30 ℃, is made primary inclined plane and is cultivated; After 48 hours, be inoculated into and shake in the bottle, 30 ℃, do secondary liquid oscilaltion and cultivate; After 36 hours, be 5% inoculum size, be inoculated on the solid medium, 30 ℃, do three grades and be cultured to the bacterium number and reach 1.5 * 10 with weight ratio ⁸Individual/the gram solid medium.

The preparation of solid medium: the composition of the solid medium of above-mentioned five kinds of bacterial strains and weight percent are: rice bran 50%, wheat bran 20%, Semen Maydis powder 15%, soyflour 12%, lime powder 2.9%, ammonium sulfate 0.1%.Before the use, regulate the moisture content to 45% in the substratum, 121 ℃, moist heat sterilization 30 minutes is cooled to room temperature.

The mixing of bacterial strain pure growth: with each bacterial strain pure growth with mass ratio 2: 1: 1: mix, just obtained of the present invention microbiobacterial agent at 1: 1.

In addition feces of livestock and poultry is placed fermenter, add 40% straw powder mixing by weight, regulate moisture content to 45%, obtain the pre-treatment material.At room temperature, be that 5% inoculum size adds the mentioned microorganism microbial inoculum with weight ratio in the pre-treatment material, stir, and aeration regularly, can reach fully in 10 days and become thoroughly decomposed, water content is reduced to about 20%, and the material that becomes thoroughly decomposed is a grey, slightly the vinasse smell.

Embodiment 2

1 ton of freshly-slaughtered poultry excrement (water content 45～70%) is pressed after the method pre-treatment among the embodiment 1, the mentioned microorganism microbial inoculum that adds 0.05 ton, in the room temperature bottom fermentation, after 3 days, temperature can reach 50 ℃, regularly stirs and ventilation, does not make leavening temperature surpass 70 ℃, fermenting process finishes in 10 days, obtains the slightly leavened prod of vinasse smell of grey.With the contrast of spontaneous fermentation group, the result shows, adds microbiobacterial agent of the present invention, and fermentation period foreshortened to for 1 week by 1 first quarter moon; The product water content is reduced to about 20% by 40%; The outward appearance of product and smell are respectively grey and vinasse smell slightly, are different from the black and the mould fishy smell of spontaneous fermentation product; The product nitrogen content is 1.5 times of spontaneous fermentation, and a little less than fermentation materials not, wherein ammonia-state nitrogen has loss; Phosphorus and potassium content are suitable, and all with not fermentation materials is suitable; The product height that the organic content of product makes than spontaneous fermentation is a little less than fermentation materials not.

Claims

1. microbiobacterial agent, it is characterized in that forming by separating the refined Rockwell yeast of fat, trichoderma viride, high temperature genus bacillus, living black streptomycete, five kinds of bacterial strains of Nocardia bacteria and solid medium, it is 15～30: 20～40 that this five kinds of bacterial strains bacterium in every gram microbial inoculum is counted the scope ratio: 15～30: 15～30: 15～30.

2. microbiobacterial agent as claimed in claim 1, it is characterized in that described solid medium is made up of rice bran, wheat bran, Semen Maydis powder, soyflour, lime powder and ammonium sulfate, their weight percentage scope respectively is: 45～60%, 15～25%, 10～20%, 7～15%, 2～5%, 0.05～0.1%.

3. the preparation method of the described microbiobacterial agent of claim 1, it is characterized in that at first will separating the refined Rockwell yeast of fat, trichoderma viride, high temperature genus bacillus, give birth to black streptomycete, Nocardia bacteria does successively respectively that primary inclined plane is cultivated, secondary liquid oscilaltion is cultivated, three grades of solid fermentations are cultivated, the bacterium number to every gram solid medium reaches 0.5～1.2 * 10 respectively ⁸Individual, 1.0～5.0 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual, 1.0～2.5 * 10 ⁸Individual; Described solid fermentation is cultivated and is carried out on solid medium, before the use, regulates moisture content to 40～50%, 121～122 ℃ in the substratum, and moist heat sterilization 30～40 minutes is cooled to room temperature; At last, each bacterial strain pure growth is at room temperature counted scope than 15～30 by the bacterium of single bacterial strain: 20～40: 15～30: 15～30: 15～30 mix.

4. the described microbiobacterial agent of claim 1 is as manure treating agent.