CN101531969A - Antibacterial and environment-friendly deodorant of microorganism strains - Google Patents
Antibacterial and environment-friendly deodorant of microorganism strains Download PDFInfo
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- CN101531969A CN101531969A CN200810014748A CN200810014748A CN101531969A CN 101531969 A CN101531969 A CN 101531969A CN 200810014748 A CN200810014748 A CN 200810014748A CN 200810014748 A CN200810014748 A CN 200810014748A CN 101531969 A CN101531969 A CN 101531969A
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Abstract
The invention relates to an antibacterial and environment-friendly deodorant of microorganism strains and a preparation method thereof. The traditional method for removing odor generally uses essence to cover up the odor or pumping odor physically or uses ozone to oxidize and degrade the odor or uses a chemical method. The methods also can alleviate the odor to a certain extent, but finally the problem can not be solved really and fundamentally and the essence and chemical deodorization also have secondary pollution. The microbial deodorization technology utilizes the effects of high-efficient absorption, absorption and metabolism of particular microorganism strains which can convert or degrade odorant, to purify the odorous gases of amyl mercaptan, benzene methanethiol, methyl sulfide, ethanethiol, hydrogen sulfide, sulfur dioxide, p-benzyl mercaptan, ammonia, dimethylamine, trimethylamine and carbon disulfide and the like which are emitted from human life areas, domestic sewage and domestic garbage and the like and to convert the odorous components of amyl mercaptan, benzene methanethiol, methyl sulfide, ethanethiol, hydrogen sulfide, sulfur dioxide, p-benzyl mercaptan, ammonia, dimethylamine, trimethylamine and carbon disulfide and the like into matters without harm and odor, thus achieving the purpose of thoroughly improving air quality and protecting health of people. The preparation method utilizes a fermentation method of microorganism strains to prepare deodorant products.
Description
Technical field:
The present invention relates to a kind of antibacterial and environment-friendly deodorant agent and preparation method of microorganism fungus strain.
Background technology:
Stench is many-sided to people's influence.It not only makes a large amount of pathophorous insects, bacteria breed procreation, and directly by olfactory system, respiratory system, neural system, the recycle system, endocrine system is produced the strong impulse effect.The effect of short period of time, the sense that engenders a repugnance, feel sick, symptom such as vomiting, stimulation can cause serious symptoms such as endocrine disturbance, cardiovascular disorder for a long time.
Traditional elimination peculiar smell method is decomposed peculiar smell often with the essence covering smell or the mode of bleeding with physics, or with ozone oxidation, or is used chemical process.
Although these methods also can make peculiar smell obtain to a certain degree mitigation, it finally can not fundamentally real dealing with problems, and also there are secondary pollution in essence and chemical deodorizing.
Microbial deodorant technology of the present invention is the sulfur-bearing that utilizes efficient absorption, absorption and the metabolism of the peculiar microorganism fungus strain of the repugnant substance that can transform or degrade that sanitary sewage and domestic refuse etc. are distributed, contain foul gas such as ammonia purifies; odor pollutants such as hydrogen sulfide, mercaptan and ammonia are converted into the material of harmless odorless, reach the thorough target of improving Air quality, protection people's health
Summary of the invention:
The ultimate principle of microbiological treatment foul smell of the present invention is to utilize microorganism that the repugnant substance in the dissolving water is absorbed in microorganism in health, makes a kind of process of its degraded by the Metabolic activity of microorganism.Basically be divided into three processes: the 1. dissolution process of foul gas, promptly changed into the mass transfer process of liquid phase by gas phase; 2. foul smell soluble in water is absorbed by microorganism by microbial cell wall and cytolemma, and water-fast foul smell is earlier attached to outside the microbe, is decomposed into soluble substance by the perienzyme of microorganism secretion, infiltrates cell again; 3. after foul smell enters cell, be that Institute of Micro-biology decomposes, utilizes, makes foul smell to be removed as nutritive substance in vivo.The biological degradation of repugnant substance is the rate-limiting step of this process, and visible microorganism is in the core status of biological deodorization.Microorganism digests and assimilates the nutriment that the metabolite that produces behind the repugnant substance is re-used as other microorganisms, continues to assimilate, and so circulation is progressively degraded repugnant substance.Fungal growth speed is fast, and the mycelia net of formation can effectively increase the contact area with gas, is applicable to multiple insoluble foul smell.
The technical solution used in the present invention is:
1, strain separating screening
Adopt 3 kinds of methods to separate: (1) line partition method, promptly to rule at the solid culture primary surface with inoculating needle picking microbiological specimens, felicity condition is cultivated down, obtains single bacterium colony; (2) coating partition method, spreading rod dips in gets nutrient solution, or earlier a small amount of nutrient solution is dropped in the solid culture primary surface, and the solid medium surface coated is even again with spreading rod again; (3) isolation by dilution method, it is bigger to obtain purebred probability, after this method is the solid medium and a certain amount of bacteria suspension mixing of will reduce to about 50 ℃, pours into dull and stereotyped to obtain single bacterium colony again.
Filtered out 80 strains altogether by primary election and final election and had the bacterial strain of deodorizing capability by force, wherein bacterium, filamentous fungus, actinomycetes, yeast are respectively 35,15,10,20 strains.
The major microorganisms kind classification of the deodorizing microorganism fungus strain that is separated to is:
(1) bacterium class: Alkaligenes (Alcaligeaes), Rhodopseudomonas Latin formal name used at school [Rhodopseudomonas (Kluyver and van Niel) Czurdaand Maresch, 1937] (photosynthetic bacterium class Rhodopseudomonas), lactobacillus (lactic acid bacteria class Lactobacillus), corynebacterium (Corynebacterium), acetic acid acetobacter (Acetobactcr aceti).
(2) budding fungus: bacterial classification candida tropicalis Candida tropicalis (Cast.), yeast belong (Saccharomyces);
(3) filamentous fungus class: Rhizopus (Rhizopus), Mucor (Mucoraceae), Aspergillus (Aspergillus);
(4) ray fungi: Nocardia (Nocardia).
The deodorizing bacterial strain of the present invention's screening mainly is photosynthetic bacterium class, acetobacter class, Bacterium lacticum class, bacillus class, Rhodopseudomonas, yeast, filamentous fungus and ray fungi.
2, mixed bacterium liquid extension zymotechnique flow process:
3, the selection of substratum and kind
At different microorganisms and different nutritional requirements, different substratum can be arranged, but culture medium preparation must be followed certain principle: (1) nutritive substance should satisfy the needs of microorganism; (2) concentration of nutrition and proportioning should be appropriate; (3) Shi Yi pH; (4) meet the purpose of cultivation.Except that time, the content, sterilization that also should be taken into account impurity in the viscosity, raw material of substratum whether easily and thoroughly, sterilization back nutrition destructiveness etc., they are all to thalli growth with product is synthetic exerts an influence.We are according to the characteristic of used bacterial classification and fermentation purposes thing, additives such as nutrition such as carbon source, nitrogenous source, inorganic salt, somatomedin and promotor, buffer reagent have been carried out one-factor experiment one by one, understand the influence of these factor pair thalli growths and product property, combine the mutual relationship of various factors, carry out orthogonal test, draw the composition and the preparation of following substratum:
4, strain preparation scheme and seed quality measure of control: (1) is being seeded to triangular flask with actinomycetes, yeast, acetobacter, and shaking table is cultivated; Shaking speed 150r/min, temperature is controlled at 28 ℃-30 ℃, and total count content reaches 1 * 10
8The order of magnitude stops cultivating.(2) cultivation of photosynthetic bacterium: photosynthetic bacterium is seeded to the thin mouthful vial (2L) of semi closed, room temperature, illumination (800Lux) is cultivated, and the gap is shaken.(3) cultivation of milk-acid bacteria: in the extremely airtight thin mouthful of vial of lactobacillus inoculum, 28 ℃ of static cultivations down.
On the basis of single bacterium culture technique of research and selected deodorizing microorganism mutualism, create microbial deodorant fungus strain bacterial classification and the fermentation system of oneself.
The big jar of general technological process of production of solution fermentation is: bacterial classification inoculation is amassed wealth by heavy taxation foster → seed tank culture → production jar cultivation → discharging nutrient solution and is added an amount of carrier → environment-friendly deodorant fungus strain product
5, the culture condition of mixed bacterium
In the culturing process of deodorizing mixed bacterium, for the amount of obtaining is big rapidly, inexpensive high reactivity cell must be created a good living environment for it, such as: suitable humidity, pH value, oxygen content, temperature and nutritive ingredient etc.Control relative humidity remains on 80%～90% in the test.
The present invention has carried out the test of biological deodorizing fungus strain industrial fermentation, and the result shows: lemon 5%, peppermint 2%, Herba Cymbopogonis Citrari 4%, Rosmarinus officinalis 1%, brown rice vinegar 18%, molasses 20%, pure water 50%, pH value are 3-5, and growth temperature is 18-35 ℃.Industrial fermentation nutrient solution as the Black Liquor with Efficient Bacteria of deodorization functions.
Embodiment:
In the synthetic medium of seed fermentation liquid with each bacterial strain in the ratio access mixed bacterium growth of bacterium class 15%, budding fungus 20%, filamentous fungus class 30%, ray fungi 35%, ferment according to above-mentioned industrial fermentation flow process, the viable count of monitoring simultaneously in the fermenting process in the fermented liquid is that total count reaches 1 * 10 in the fermented liquid
8The order of magnitude.Stop fermentation as product stoste.
Application aspect environment-friendly deodorant
(1) aquaculture
Adopt dual mode to test at aquaculture, 1, be added in the feed with the form of additive, to increase digesting and assimilating of feedstuff protein, reduce the foul smell discharging.2, raise the place for 100 times-300 times with stoste dilution and directly spray, stench has for many years faded away, and fly density descends greatly, and it is docile, quiet that livestock and poultry become, and lays eggs, meat productivity obviously increases.
(2) application in domestic rubbish disposal
At the domestic refuse of disposal site and family's generation every day, spray deodorizing with 100 times of dilutions of stoste, 2-5 minute moment can be removed peculiar smell.
(3) application in industrial production
At the peculiar smell that paper mill, feed factory, chemical enterprise etc. produce in process of production, use this microorganism fungus strain stoste to dilute 100 times of diffusion deodorizing that atomize and obtain good result.
(4) public place is used
To shopping center, school, hospital, the home for the aged, kindergarten, laundry, cinema, Public toilets, toilet, Karaoke room, public place of entertainment, health-care facilities, museum of new and advanced technology exchanging meeting, hotel guest-room, hotel's parlor, fitting space, zoological park etc., test shows to have good deodorizing freshness effect.
(5) application of domestic hygiene aspect
This project product can be used for deodorizing in the family room, and the objectionable impurities that contains in to finishing material through test (pentan-thiol, beneze methane thiol, dimethyl sulfide, sulfur alcohol, hydrogen sulfide, sulfurous gas, right-benzyl mercaptan, ammonia, dimethylamine, Trimethylamine 99, dithiocarbonic anhydride etc.) has good decomposition and inversion effect.And germicidal action preferably arranged.
Claims (3)
1, a kind of antibacterial and environment-friendly deodorant of microorganism strains is multiple microbial fermentation fungus symbiotic system, it is characterized in that wherein containing:
(1) bacterium class: Alkaligenes (Alcaligeaes), Rhodopseudomonas Latin formal name used at school [Rhodopseudomonas (Kluyver and van Niel) Czurda and Maresch, 1937] (photosynthetic bacterium class Rhodopseudomonas), lactobacillus (lactic acid bacteria class Lactobacillus), corynebacterium (Corynebacterium), acetic acid acetobacter (Acetobacter aceti).
(2) budding fungus: bacterial classification candida tropicalis Candida tropicalis (Cast.), yeast belong (Saccharomyces);
(3) filamentous fungus class: Rhizopus (Rhizopus), Mucor (Mucoraceae), Aspergillus (Aspergillus);
(4) ray fungi: Nocardia (Nocardia).
Total count is 1 * 10
7-1 * 10
8The order of magnitude.
2, require described antibacterial and environment-friendly deodorant of microorganism strains according to right 1, it is characterized in that this fungus strain products production ferments in following nutrient solution:
Lemon 5%, peppermint 2%, Herba Cymbopogonis Citrari 4%, Rosmarinus officinalis 1%, brown rice vinegar 18%, molasses 20%, pure water 50%, pH value are 3-5, and growth temperature is 18-35 ℃.
3, a kind of preparation method of antibacterial and environment-friendly deodorant of microorganism strains
Mixed bacterium liquid extension zymotechnique flow process:
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