CN102586145A - Garbage fermentation agent - Google Patents
Garbage fermentation agent Download PDFInfo
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- CN102586145A CN102586145A CN2012100306037A CN201210030603A CN102586145A CN 102586145 A CN102586145 A CN 102586145A CN 2012100306037 A CN2012100306037 A CN 2012100306037A CN 201210030603 A CN201210030603 A CN 201210030603A CN 102586145 A CN102586145 A CN 102586145A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention discloses a garbage fermentation agent which comprises the following bacterial broth in volume ratio: 10-40 percent of bacillus subtilis, 5-25 percent of acetic acid bacteria, 5-15 percent of bacillus polymyxa, 5-20 percent of aspergillus niger, 1-8 percent of nocardia, 3-10 percent of trichoderma viride and 5-15 percent of saccharomycetes. The garbage fermentation agent disclosed by the invention has the advantages that environment malodor is eliminated and the multiplication of harmful bacteria is inhibited; organic garbage is subjected to composting treatment and resources are recycled, so that the environmental pollution is reduced; and dirt in a sewer is reduced, and thus the odor is reduced. The garbage fermentation agent disclosed by the invention is suitable for being widely applied to fermentation of various kinds of garbage.
Description
Technical field
The present invention relates to a kind of rubbish fermentation agent, belong to the refuse treatment field.
Background technology
Along with the development of Population Urbanization, domestic waste output is also increased sharply simultaneously, how scientifically to handle domestic waste, has become the important topic of pendulum in face of governments at all levels.Because it not only influences city look and feel, more serious is to cause environmental pollution, the quality of life that influences the masses of the people, jeopardizes public health and economic Sustainable development, and this is antipathetic with building harmonious health social.
Want science to handle domestic waste, must understand fully its main moity earlier.The staple of forming domestic waste has coal ash, kitchen slag, pericarp, plastics, fallen leaves, fabric, timber, glass, pottery, leather and paper and a spot of battery, pharmaceutical pack material of aluminum foil, SP composite package/bag, rubber etc.; And these divide into groups to change also apparent in view with changing season in northern city.Because China is vast in territory, Economic development is uneven, and living condition and custom difference are huge, and the domestic refuse composition that is therefore produced also has very big difference.The high city of economically developed, two gas rates, garbage organism content is high, otherwise then lower.
The refuse treatment processing mode of China's employing at present mainly adopts the sanitary landfill mode, adopts burning electricity generation, comprehensive utilization, composting mode to handle on a small quantity.Different modes respectively has characteristics, is fit to various conditions, and different cities will adopt the treatment process that is fit to local characteristics with the area.
The sanitary landfill mode is that rubbish is inserted compacting in the ready hole, and earthing is buried, and makes it that biology, physics, chemical transformation take place, and decomposing organic matter reaches minimizing and innoxious purpose.
Above-mentioned treatment process cost is high, can cause further pollution to environment, and therefore a kind of waste disposal method efficiently is provided, and reduces second environmental pollution, has enormous social.
Summary of the invention
The purpose of this invention is to provide a kind of pollution-free, efficient, safe mikrobe rubbish fermentation agent.
For achieving the above object, the present invention adopts following steps:
Described starter is mixed by microbial fermentation solution.The microbial fermentation solution volume ratio is:
The subtilis of 10-40%; The acetic bacteria of 5-25% (Acetobacter); The bacillus polymyxa of 5-15% (Paenibacillus polymyxa); 5-20% black mold (Aspergillusniger), the Nocardia bacteria of 1-8%, 3-10% viride (Trichodermaviride), 5-15% yeast.
Preferably; Described microbial fermentation solution volume ratio is: the subtilis of 20-35%; The acetic bacteria of 10-22%; The bacillus polymyxa of 6-14%, 8-18% black mold (Aspergillusniger), the Nocardia bacteria of 2-6%, 4-8% viride (Trichodermaviride), 8-13% yeast.
Further preferred; Described microbial fermentation solution volume ratio is: 35% subtilis, 20% acetic bacteria, 12% bacillus polymyxa; 13% black mold (Aspergillusniger), 4% Nocardia bacteria, 6% viride (Trichodermaviride), 10% yeast.
Described Nocardia bacteria is preferably the glanders Nocardia bacteria.Described yeast is preferably yeast saccharomyces cerevisiae (Saccharomyces cerevisiae).
Described mikrobe classification is respectively cultivated, and then the fermented liquid proportional mixing is promptly got.
Black mold
Seed culture: the black mold of purifying is seeded in the Cha Shi substratum, 32-37 ℃, cultivated 3-5 days.
Fermentation culture: substratum (weight ratio): straw powder 3%, molasses 1%, wheat bran 1%, soybean cake powder 3%, sodium-chlor 0.3%, surplus is a water; 28-35 ℃, cultivated 5-7 days.
Viride
Seed culture: the viride of purifying is seeded in the Cha Shi substratum, 32-37 ℃, cultivated 3-5 days.
Fermentation culture: substratum (weight ratio): straw powder 3%, molasses 1%, wheat bran 1%, soybean cake powder 3%, sodium-chlor 0.3%, surplus is a water; 28-35 ℃, cultivated 5-7 days.
Bacillus polymyxa
One-level is cultivated
Glucose 1.5%, wheat bran filtrating 1%, potassium primary phosphate 0.4%, ammonium chloride 0.1%, SODIUM PHOSPHATE, MONOBASIC 0.5%, sodium-chlor 0.04%, agar 3% was cultivated 2-3 days for excess water 25-27 ℃.
Secondary medium
Glucose 3.3%, meat peptone 1%, fish peptone 0.5%, sodium-chlor 0.3%, bubble enemy 0.01%, lime carbonate 0.5%, surplus is a water.PH value 6.3-6.7 cultivated 3-5 days for 25-27 ℃.
Three-stage culture medium
Medium component is by percentage to the quality:
W-Gum 16%, ammonium sulfate 1.3%, steeping water 0.4%, L-aspartic acid 0.2%, 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, surplus are water.Cultivated 3-5 days for 25-27 ℃.
Subtilis
One-level is cultivated
Conventional yam adds nutrient agar, cultivates 2-3 days for 25-27 ℃.
Secondary is cultivated
Remove agar, cultivated 3-5 days for 25-27 ℃.
Three grades of cultivations
Medium component is by percentage to the quality: 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, surplus are water.Cultivated 5-7 days for 25-27 ℃.
Acetic bacteria
One-level is cultivated
Sucrose 1.5%, potassium primary phosphate 0.05%, lime carbonate 0.05%, sal epsom 0.05%, sodium-chlor 0.02%, yeast extract paste 0.05%, agar 3%, surplus is a water.Cultivated 2-3 days for 25-27 ℃.
Secondary is cultivated:
The one-level substratum removes agar, cultivates 3-5 days for 25-27 ℃.
Three-stage culture medium
Medium component is by percentage to the quality: 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash.Adjust pH is seeded in the secondary cultured strain in the seeding tank, cultivates 5-7 days for 25-27 ℃.
Nocardia bacteria
One-level is cultivated
Medium component is by percentage to the quality: 0.5% yeast extract paste, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, PH6 ~ 8, temperature 28-32 ℃, time 2-5 days.
Secondary is cultivated
The one-level substratum adds 0.1% ferric ammonium citrate, and culture condition is cultivated with one-level.
Three grades of cultivations
The secondary cultured strain is seeded in the seeding tank, temperature 28-32 ℃, time 5-7 days.
Substratum: glucose 1-3%, yeast water 1-2%, potassium primary phosphate 0.01-0.05%, sodium-chlor 0.02-0.05%, ferrous sulfate 0.001-0.005%, surplus is a water.
Yeast
One-level is cultivated
Medium component is by percentage to the quality: 0.5% yeast extract paste, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, PH6 ~ 8, temperature 28-32 ℃, time 2-5 days.
Secondary is cultivated
The one-level substratum adds 0.1% ferric ammonium citrate, and culture condition is cultivated with one-level.
Three grades of cultivations
The secondary cultured strain is seeded in the seeding tank, temperature 28-32 ℃, time 5-7 days.
Substratum: glucose 1-3%, yeast water 1-2%, potassium primary phosphate 0.01-0.05%, sodium-chlor 0.02-0.05%, ferrous sulfate 0.001-0.005%, surplus is a water.
After the mentioned microorganism fermented liquid proportionally prepared, mixed bacteria liquid evenly is sprayed on the rubbish, the volume ratio of starter and rubbish is 1-2:400-500, gets final product after stirring.
Through detecting, starter bacterial count of the present invention is more than 30,000,000,000/ml.
Above-mentioned fermented liquid also can add sorbent material, vehicle, is prepared into solid fungicide after the cryodrying.
Starter of the present invention also can be used to handle water drain, organic garbage of city etc., in order to preparation fertilizer.
NM parameter or method among the present invention are this area ordinary method.
Beneficial effect:
1, eliminates environment stench, inhibition unwanted bacteria propagation;
2, organic waste composting, the recycle resource reduces environmental pollution;
3, reduce the water drain dirt, alleviate stink.
Table 1 is that the composition before and after each rubbish fermentation changes (the oven dry back is measured)
Table 1 rubbish fermentation component content changes contrast
Can know that through table 1 rubbish is after fermentation, each change of component is obvious.Starter of the present invention has tangible effect to the degraded of various daily rubbish.
Embodiment
Embodiment 1
Black mold
Seed culture: the black mold of purifying is seeded in the Cha Shi substratum, 32-37 ℃, cultivated 3-5 days.
Fermentation culture: substratum (weight ratio): straw powder 3%, molasses 1%, wheat bran 1%, soybean cake powder 3%, sodium-chlor 0.3%, surplus is a water; 28-35 ℃, cultivated 5-7 days.
Viride
Seed culture: the viride of purifying is seeded in the Cha Shi substratum, 32-37 ℃, cultivated 3-5 days.
Fermentation culture: substratum (weight ratio): straw powder 3%, molasses 1%, wheat bran 1%, soybean cake powder 3%, sodium-chlor 0.3%, surplus is a water; 28-35 ℃, cultivated 5-7 days.
Bacillus polymyxa
One-level is cultivated
Glucose 1.5%, wheat bran filtrating 1%, potassium primary phosphate 0.4%, ammonium chloride 0.1%, SODIUM PHOSPHATE, MONOBASIC 0.5%, sodium-chlor 0.04%, agar 3% was cultivated 2-3 days for excess water 25-27 ℃.
Secondary medium
Glucose 3.3%, meat peptone 1%, fish peptone 0.5%, sodium-chlor 0.3%, bubble enemy 0.01%, lime carbonate 0.5%, surplus is a water.PH value 6.3-6.7 cultivated 3-5 days for 25-27 ℃.
Three-stage culture medium
Medium component is by percentage to the quality:
W-Gum 16%, ammonium sulfate 1.3%, steeping water 0.4%, L-aspartic acid 0.2%, 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, surplus are water.Cultivated 3-5 days for 25-27 ℃.
Subtilis
One-level is cultivated
Conventional yam adds nutrient agar, cultivates 2-3 days for 25-27 ℃.
Secondary is cultivated
Remove agar, cultivated 3-5 days for 25-27 ℃.
Three grades of cultivations
Medium component is by percentage to the quality: 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, surplus are water.Cultivated 5-7 days for 25-27 ℃.
Acetic bacteria
One-level is cultivated
Sucrose 1.5%, potassium primary phosphate 0.05%, lime carbonate 0.05%, sal epsom 0.05%, sodium-chlor 0.02%, yeast extract paste 0.05%, agar 3%, surplus is a water.Cultivated 2-3 days for 25-27 ℃.
Secondary is cultivated:
The one-level substratum removes agar, cultivates 3-5 days for 25-27 ℃.
Three-stage culture medium
Medium component is by percentage to the quality: 0.5% sodium-acetate, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash.Adjust pH is seeded in the secondary cultured strain in the seeding tank, cultivates 5-7 days for 25-27 ℃.
Nocardia bacteria
One-level is cultivated
Medium component is by percentage to the quality: 0.5% yeast extract paste, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, PH6 ~ 8, temperature 28-32 ℃, time 2-5 days.
Secondary is cultivated
The one-level substratum adds 0.1% ferric ammonium citrate, and culture condition is cultivated with one-level.
Three grades of cultivations
The secondary cultured strain is seeded in the seeding tank, temperature 28-32 ℃, time 5-7 days.
Substratum: glucose 1-3%, yeast water 1-2%, potassium primary phosphate 0.01-0.05%, sodium-chlor 0.02-0.05%, ferrous sulfate 0.001-0.005%, surplus is a water.
Yeast
One-level is cultivated
Medium component is by percentage to the quality: 0.5% yeast extract paste, 0.5% peptone, 0.05% sodium-chlor, 0.05% potassium hydrogenphosphate, 0.05% sal epsom, 0.05% yellow soda ash, PH6 ~ 8, temperature 28-32 ℃, time 2-5 days.
Secondary is cultivated
The one-level substratum adds 0.1% ferric ammonium citrate, and culture condition is cultivated with one-level.
Three grades of cultivations
The secondary cultured strain is seeded in the seeding tank, temperature 28-32 ℃, time 5-7 days.
Substratum: glucose 1-3%, yeast water 1-2%, potassium primary phosphate 0.01-0.05%, sodium-chlor 0.02-0.05%, ferrous sulfate 0.001-0.005%, surplus is a water.
Embodiment 2
Fermented liquid is carried out proportioning by following volume ratio:
40% subtilis, 21% acetic bacteria, 5% bacillus polymyxa, 20% black mold, 1% Nocardia bacteria, 3% viride, 10% yeast.
After the mentioned microorganism fermented liquid proportionally prepared, mixed bacteria liquid evenly is sprayed on the rubbish, the volume ratio of starter and rubbish is 1:400, gets final product after stirring.
Embodiment 3
35% subtilis, 20% acetic bacteria, 10% bacillus polymyxa, 10% black mold, 6% Nocardia bacteria, 4% viride, 15% yeast.
After the mentioned microorganism fermented liquid proportionally prepared, mixed bacteria liquid evenly is sprayed on the feces of livestock and poultry, the volume ratio of starter and feces of livestock and poultry is 1:500, gets final product after stirring.
Embodiment 4
36% subtilis, 20% acetic bacteria, 12% bacillus polymyxa, 18% black mold, 1% Nocardia bacteria, 8% viride, 5% yeast.
After the mentioned microorganism fermented liquid proportionally prepared, mixed bacteria liquid evenly is sprayed on the feces of livestock and poultry, the volume ratio of starter and feces of livestock and poultry is 1:400, gets final product after stirring.
Embodiment 5
The microbial fermentation solution volume ratio is: 35% subtilis, 20% acetic bacteria, 12% bacillus polymyxa, 13% black mold, 4% Nocardia bacteria, 6% viride, 10% yeast.
After the mentioned microorganism fermented liquid proportionally prepared, mixed bacteria liquid evenly is sprayed on the feces of livestock and poultry, the volume ratio of starter and feces of livestock and poultry is 2:500, gets final product after stirring.
Claims (5)
1. rubbish fermentation agent comprises the microbial fermentation solution of following volume ratio: the subtilis of 10-40%, the acetic bacteria of 5-25%, the bacillus polymyxa of 5-15%, 5-20% black mold, the Nocardia bacteria of 1-8%, 3-10% viride, 5-15% yeast.
2. starter according to claim 1 is characterized in that, described microbial fermentation solution volume ratio is: the subtilis of 20-35%; The acetic bacteria of 10-22%; The bacillus polymyxa of 6-14%, 8-18% black mold, the Nocardia bacteria of 2-6%, 4-8% viride, 8-13% yeast.
3. starter according to claim 2 is characterized in that, described microbial fermentation solution volume ratio is: 35% subtilis; 20% acetic bacteria, 12% bacillus polymyxa, 13% black mold; 4% Nocardia bacteria, 6% viride, 10% yeast.
4. according to claim 1,2 or 3 described starters, it is characterized in that described microbial fermentation solution bacterial count is more than 30,000,000,000/ml.
5. according to claim 1,2 or 3 described starters, it is characterized in that the volume ratio of starter and feces of livestock and poultry is 1-2:400-500.
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| CN2012100306037A CN102586145A (en) | 2012-02-13 | 2012-02-13 | Garbage fermentation agent |
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| CN2012100306037A CN102586145A (en) | 2012-02-13 | 2012-02-13 | Garbage fermentation agent |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747065A (en) * | 2012-07-30 | 2012-10-24 | 宜春强微生物科技有限公司 | High-efficiency fermenting agent for poultry excrement and fermenting method of fermenting agent |
| CN103088072A (en) * | 2013-01-31 | 2013-05-08 | 东莞市粤源包装有限公司 | Method for automatic treatment of organic household garbage |
| CN103272361A (en) * | 2013-05-15 | 2013-09-04 | 钱臻 | Active biological complexing agent for degradation and digestion of organic wet garbage and preparation method of active biological complexing agent |
| CN103468614A (en) * | 2013-09-16 | 2013-12-25 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN111635871A (en) * | 2020-06-01 | 2020-09-08 | 上海滢弘环保科技有限公司 | Preparation method of biological full-degradable kitchen waste FDM (fused deposition modeling) microbial inoculum |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747065A (en) * | 2012-07-30 | 2012-10-24 | 宜春强微生物科技有限公司 | High-efficiency fermenting agent for poultry excrement and fermenting method of fermenting agent |
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| CN103272361B (en) * | 2013-05-15 | 2015-11-04 | 钱臻 | A kind of organic wet refuse degraded is dissolved active bio-compounding agent and preparation method thereof |
| CN103468614A (en) * | 2013-09-16 | 2013-12-25 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN103468614B (en) * | 2013-09-16 | 2015-05-20 | 中国人民解放军总后勤部军需装备研究所 | Kitchen waste decomposition bacterial agent and preparation method thereof |
| CN111635871A (en) * | 2020-06-01 | 2020-09-08 | 上海滢弘环保科技有限公司 | Preparation method of biological full-degradable kitchen waste FDM (fused deposition modeling) microbial inoculum |
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Application publication date: 20120718 |