CN104611268A - Pesticide-degrading bacterium taking bacillus amyloliquefaciens as main body as well as preparation method and application thereof - Google Patents
Pesticide-degrading bacterium taking bacillus amyloliquefaciens as main body as well as preparation method and application thereof Download PDFInfo
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- 241000193744 Bacillus amyloliquefaciens Species 0.000 title claims abstract description 48
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 241000894006 Bacteria Species 0.000 title abstract description 9
- 230000015556 catabolic process Effects 0.000 claims abstract description 50
- 238000006731 degradation reaction Methods 0.000 claims abstract description 50
- 241000193755 Bacillus cereus Species 0.000 claims abstract description 18
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 8
- 230000001580 bacterial effect Effects 0.000 claims description 50
- 239000003795 chemical substances by application Substances 0.000 claims description 41
- 241000588986 Alcaligenes Species 0.000 claims description 17
- 241000194107 Bacillus megaterium Species 0.000 claims description 17
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 17
- 241000186221 Cellulosimicrobium cellulans Species 0.000 claims description 17
- 229940097012 bacillus thuringiensis Drugs 0.000 claims description 17
- 239000002023 wood Substances 0.000 claims description 6
- 244000005700 microbiome Species 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims 1
- 239000010813 municipal solid waste Substances 0.000 abstract description 27
- 238000002156 mixing Methods 0.000 abstract description 5
- 239000012880 LB liquid culture medium Substances 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 2
- 239000002054 inoculum Substances 0.000 abstract 2
- 238000001035 drying Methods 0.000 abstract 1
- 230000002349 favourable effect Effects 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000010794 food waste Substances 0.000 description 30
- 238000000034 method Methods 0.000 description 18
- 230000008569 process Effects 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000010413 gardening Methods 0.000 description 9
- 239000001963 growth medium Substances 0.000 description 7
- 239000004382 Amylase Substances 0.000 description 6
- 102000013142 Amylases Human genes 0.000 description 6
- 108010065511 Amylases Proteins 0.000 description 6
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 6
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- 229940106157 cellulase Drugs 0.000 description 5
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- 239000002068 microbial inoculum Substances 0.000 description 5
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- 229920001817 Agar Polymers 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 3
- 230000002255 enzymatic effect Effects 0.000 description 3
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- 238000012216 screening Methods 0.000 description 3
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- 229920003023 plastic Polymers 0.000 description 2
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- 239000007787 solid Substances 0.000 description 2
- HNBDQABBWNOTRU-UHFFFAOYSA-N thalline Chemical compound C1=CC=[Tl]C=C1 HNBDQABBWNOTRU-UHFFFAOYSA-N 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
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- 102000004190 Enzymes Human genes 0.000 description 1
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- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
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- 229920002472 Starch Polymers 0.000 description 1
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
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- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
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- 235000009508 confectionery Nutrition 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/50—Reuse, recycling or recovery technologies
- Y02W30/78—Recycling of wood or furniture waste
Landscapes
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to a preparation method of a domestic garbage pesticide-degrading bacterium taking bacillus amyloliquefaciens as main body and an application in domestic garbage degradation. The strain in the domestic garbage pesticide-degrading bacterium is mainly bacillus amyloliquefaciens and additionally comprises bacillus cereus, cellulomonos and the like. The preparation method of the pesticide-degrading bacterium comprises the following steps: respectively inoculating the strains stored at 4 degrees centigrade in an LB liquid culture medium with the inoculum size of 0.5% (v/v), culturing for 24 hours at 30 degrees centigrade, and respectively transferring to inoculate in a new LB liquid culture medium to culture for 24-36 hours, wherein the inoculum size is 1% (v/v), centrifugally collecting the strain after culturing, and uniformly mixing with the wheat bran which are sterilized and baked at a high temperature after vacuum freezing and drying to obtain the pesticide-degrading bacterium. The pesticide-degrading bacterium can be used for the treatment of the domestic garbage, and is favorable for the innocent treatment and resourcezation of the garbage, and also is low in production cost, convenient to use and genetically stable.
Description
Technical field
The invention belongs to microorganism field, particularly a kind of degradation bacterial agent based on bacillus amyloliquefaciens and preparation method thereof and the application in domestic refuse degraded thereof.
Background technology
China is developing country that is populous, per capita resources shortage, but garbage as resource work progress is but very slow.How realizing garbage as resource and reduction garbage treatment quantity, is the pressing issues that China is faced with.
Changing food waste makes a general reference the food residues after the food residues and dietary consumption produced in food processing process, produces relatively concentrated, is easy to pooling of resources process.Changing food waste generally results from enterprises and institutions, school, the market of farm produce, supermarket, and food service industry etc., be the important component part of domestic waste.Along with the raising of China's city living standards of the people, the generation of changing food waste increases sharply.In recent years, the year amount of clearing of China's changing food waste has reached more than 5,000 ten thousand tons, each large main cities changing food waste day generation all more than 1000 tons.The pollution that changing food waste causes has become the subject matter of city environmental pollution, the orthobiosis of serious threat people and healthy.The harm of changing food waste has: affect the city appearance of the city and people's living environment, recycled wood materials can produce harm to human life health and changing food waste be caused the appearance of " swill pig " as forage feed domestic animal, harm food safety, brings hidden danger etc. to HUMAN HEALTH.Changing food waste is nutritious, if can in addition reasonably processing and utilizing, must turn waste into wealth, thus obtain good environmental benefit with economic benefit.Because changing food waste saliferous, oil quantity are high, be unfavorable for direct utilization, but organic content are high, have higher biodegradability, this is also for the trans-utilization of changing food waste provides feasible approach.Current most importantly biotransformation method.Bio-transformation is the metabolism utilizing microorganism self, realizes the minimizing of rubbish, resource utilization and innoxious.
The rubbish such as the resistates of local turf-mown such as gardening rubbish general reference garden, park etc. and dry branches and fallen leaves, this kind of rubbish usually can not get effective process, and especially fall leaves season in the autumn and winter, a large amount of dry branches and fallen leaves descends slowly and lightly everywhere, not only affect city look and feel, make troubles also to the trip of people.If the gardening rubbish after sanitationman's cleaning can be effectively addressed, the object of utilization of waste material can not only be realized, bring great convenience also can to the go off daily of people.
The usual water content of gardening rubbish is lower, and itself and changing food waste process by we simultaneously, utilizes gardening rubbish to regulate the water content of changing food waste, can solve the high problem of changing food waste water content to a certain extent.Because the enzyme cording of the bacillus amyloliquefaciens secretion of our screening has strong starch-splitting ability, therefore same for bacillus amyloliquefaciens bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis mixing are used for the Degradation and Transformation of domestic refuse by us as compost bacterium.At present, in process changing food waste process, the utilization of garbage disposer is more, uses sun power refuse disposal system to utilize sun power, reduce expenses, energy-conserving and environment-protective, also can reach the object of refuse treatment, realize garbage decrement, resource utilization and innoxious effect.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, a kind of degradation bacterial agent based on bacillus amyloliquefaciens and preparation method thereof and application are provided.
The object of the invention is to be achieved through the following technical solutions: a kind of degradation bacterial agent based on bacillus amyloliquefaciens, described degradation bacterial agent is made up of mixed strains and carrier, and mixed strains accounts for 10% ~ 20% of degradation bacterial agent quality; Described mixed strains comprises bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis, wherein, the mass ratio of bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is 2 ~ 4:1:1:1:1:1:1.
Further, described carrier is selected from wheat bran, dregs of beans, wood chip, and the present invention is preferably wheat bran, wood chip.
Further, the mass ratio of described bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is preferably 2:1:1:1:1:1:1.
Further, described bacillus amyloliquefaciens is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) of No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, deposit number: CGMCC No.9196, Classification And Nomenclature: bacillus amyloliquefaciens (
bacillus amyloliquefaciens), preservation date is on May 23rd, 2014.
A kind of preparation method of the degradation bacterial agent based on bacillus amyloliquefaciens, by described bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is respectively in LB substratum, 30 DEG C of constant temperature culture 24-36 hour, by bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, the nutrient solution of subtilis mixes according to volume ratio 2 ~ 4:1:1:1:1:1:1, collected by centrifugation thalline, after vacuum lyophilization, with high-temperature sterilization, carrier after oven dry mixes, the mass ratio of mixed strains and carrier is 1 ~ 2:9 ~ 8, be prepared into degradation bacterial agent.
An application for degradation bacterial agent based on bacillus amyloliquefaciens, this is applied as, and domestic refuse degradation bacterial agent is applied to the degraded of domestic refuse.
Compared with prior art, the present invention has following beneficial effect: the present invention is by mixing the mixed strains and carrier wheat bran with efficient degradation changing food waste ability, make the domestic refuse degradation bacterial agent based on bacillus amyloliquefaciens, have the following advantages for the treatment of changing food waste and gardening rubbish: bacillus amyloliquefaciens has the diastatic ability of stronger product, has good degradation effect for starchy materials such as the rice in changing food waste.Homemade degradation bacterial agent is applied to the degraded of changing food waste and gardening rubbish, degradation effect is obvious; The equal nontoxicity of each bacterial classification in mixed strains, carrier is cheap and easy to get, reduces processing cost, has broad application prospects and promotional value.Gardening rubbish water content is lower, changing food waste and gardening rubbish is mixed process and can solve the high problem of changing food waste water content to a certain extent, thus reach good treatment effect.
Embodiment
The invention provides a kind of degradation bacterial agent for the treatment of domestic refuse based on bacillus amyloliquefaciens, and utilize the method for described degradation bacterial agent process domestic refuse, described degradation bacterial agent comprises described mixed strains and carrier, described mixed strains take bacillus amyloliquefaciens as main bacteria seed, also comprises bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis in addition.
Below by specific embodiment to degradation bacterial agent of the present invention and the method using described degradation bacterial agent process domestic refuse, be described in detail and illustrate, to make better to understand the present invention, but following embodiment does not limit the scope of the invention.
embodiment 1
The bacterial classification that the present embodiment screening degradation capability is strong, to prepare degradation bacterial agent, concrete screening process is as follows:
The bacterial strain that the working foundation of bacterial isolation in early stage obtains is put respectively and is connected on proteolytic enzyme primary dcreening operation flat board, amylase primary dcreening operation flat board, lipase primary dcreening operation flat board and cellulase primary dcreening operation flat board, cultivate 24-36 hour for 30 DEG C, proteolytic enzyme primary dcreening operation flat board can directly be observed with or without transparent circle, amylase primary dcreening operation flat board need observe transparent circle with after dagger-axe formula iodine staining, cellulase primary dcreening operation flat board need observe transparent circle with after congo red staining, lipase primary dcreening operation flat board is observed bottom and whether is occurred red globules, is measured by the enzymatic productivity of aforesaid method to each bacterial strain.
Proteolytic enzyme primary dcreening operation plate culture medium used, amylase primary dcreening operation plate culture medium, lipase primary dcreening operation plate culture medium and cellulase primary dcreening operation slat chain conveyor based formulas are as follows:
Amylase primary dcreening operation plate culture medium: extractum carnis 5 g, peptone 10 g, NaCl 5 g, Zulkovsky starch 2 g, agar 20 g, water 1000mL, pH 7.0-7.2.
Proteolytic enzyme primary dcreening operation plate culture medium: extractum carnis 3 g, NaCl 5 g, casein food grade 10 g, agar 15 g, water 1000 mL, pH 7.0-7. 2.
Cellulase primary dcreening operation plate culture medium: Mandel nutritive medium, 2% carboxymethyl cellulose, yeast extract paste 0.1g, the agar of 1.5%, water 1000mL; Mandel nutritive medium consists of the following composition: NaNO
32g/L, K
2hPO
41.5g/L, CaCl
21.5g/L, MgSO
40.3g/L, FeSO
4.7H
2o 0.005g/L, MnSO
4.H
2o 0.0016g/L, ZnSO
4.H
2o 0.0014g/L, CoCl
20.0005g/L, pH 6.8-7.0.
Lipase primary dcreening operation plate culture medium: peptone 4 g, glucose 5 g, sweet oil 40 g, agar 15 g, water 1000mL, pH 7. 0 ~ 7. 2.
Wherein, proteolytic enzyme primary dcreening operation flat board directly can observe transparent circle; Amylase flat board is observed after need steaming with iodine fumersion; Lipase flat board is observed bottom and whether is occurred red globules, if any then illustrating that fat is hydrolyzed, being positive reaction, being designated as "+", otherwise is negative, is designated as "-".Cellulase flat board need with congo red staining: add 10 ml congo red staining liquid (1%, w/v), be as the criterion with submerged culture base, contaminate 30min; Dyeing terminates the method that rear sodium chloride solution (1M) wash-out and distilled water wash-out combine, and washes away residual mycelia, with sodium-chlor repeatedly to washing lotion is transparent.Observe bacterium colony size, survey transparent circle diameter, and calculate the ratio (HC) of transparent circle diameter and colony diameter.
Measured by the enzymatic productivity of aforesaid method to the bacterial classification filtered out, wherein bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis all have higher enzymatic productivity, and the hydrolysis circle of bacillus amyloliquefaciens on amylase primary dcreening operation flat board is far longer than other bacterial classifications, illustrate that bacillus amyloliquefaciens produces diastatic ability apparently higher than other bacterial classifications.
embodiment 2
According to embodiment 1, bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis have stronger degradation capability, the present embodiment is based on bacillus amyloliquefaciens, prepare degradation bacterial agent, step is as follows:
4 DEG C of bacillus amyloliquefaciens preserved, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilises are cultivated respectively in the medium, wherein, described substratum is LB substratum, the culture temperature of each bacterial classification is 30 DEG C, and incubation time is 24-36 hour.
After cultivation 24-36 hour, because each cell density difference is little, only the nutrient solution of bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis need be mixed according to volume ratio 2 ~ 4:1:1:1:1:1:1;
By mixed solution at centrifugal 6 min of 5000 rpm, collect thalline, after vacuum lyophilization, obtain microbial dry powder, then by mixed strains and high-temperature sterilization, dry after wheat bran mix, wherein, mixed strains accounts for 10% ~ 20% of degradation bacterial agent quality; Obtain the degradation bacterial agent based on bacillus amyloliquefaciens, this degradation bacterial agent is solid dry powder, and viable count is 1 × 10
8individual/gram more than.
In above-mentioned degradation bacterial agent, the carrier of described mixed strains can be fermented liquid, any one or a few mixture in substratum or other carrier that oneself knows, and in order to cost-saving, the present invention is preferably wheat bran, wood chip.
In the various mixed strainss of composite fungus agent of the present invention, each bacterial classification quantitative proportion is preferably bacillus amyloliquefaciens: bacillus cereus: cellulomonas cartae: Alcaligenes: bacillus megaterium: bacillus thuringiensis: subtilis=2:1:1:1:1:1:1.
embodiment 3
The degradation bacterial agent that embodiment 2 is prepared by the present embodiment, for the degraded of changing food waste, concrete grammar is as follows:
Degradation bacterial agent based on bacillus amyloliquefaciens is added changing food waste (to take from Zhejiang University's students' dining hall, first the foreign material such as bone, plastics bag, dixie cup are sorted out, then drain swill, fully mix.) in carry out semi-solid ferment, according to microbial inoculum: the mixing of the mass ratio of changing food waste=2 ~ 10:100, leavening temperature is 30 DEG C, and fermentation time is 5d.The degradation treatment effect (garbage degradation rate) of degradation bacterial agent embodies with the weight-loss ratio of changing food waste:
Weight-loss ratio (%)=(T1-T2)/T1
T1: drop into rubbish gross weight; T2: rubbish gross weight (dropping into the weight after changing food waste 7d) after process.
After fermentation 10d, the average weight-loss ratio going out garbage weight according to formulae discovery is 74.9%.
embodiment 4
The degradation bacterial agent that embodiment 2 is prepared by the present embodiment, for domestic refuse of degrading, concrete grammar is as follows:
Degradation bacterial agent based on bacillus amyloliquefaciens and the glad microbial inoculum of commodity digesting and absorbing the essence of foodstuff (are mainly comprised changing food waste and gardening rubbish with domestic refuse respectively, changing food waste takes from Zhejiang University's students' dining hall, first the foreign material such as bone, plastics bag, dixie cup are sorted out, then drain swill, fully mix.) according to microbial inoculum: the mass ratio mixing of domestic refuse=0.5 ~ 2:100, is placed in the sun power refuse disposal system being equipped with ventilation subsystem, ventilation 0.25m respectively
3/ (minm
3), front 13d is stuffy, rear 13d intermittent aeration (logical 5min stops 15min).
Compared with the glad microbial inoculum of commodity digesting and absorbing the essence of foodstuff, degradation bacterial agent of the present invention to the degradation rate (60.58%) of domestic refuse higher than the glad microbial inoculum of commodity digesting and absorbing the essence of foodstuff (54.80%), and utilize the heap temperature of self-control degradation bacterial agent process higher, higher heap temperature can promote complicated with decomposition that is some difficult organic compound decomposed, be more conducive to kill the pathogenic microorganism in heap body, contribute to garbage harmless and resource utilization.
Claims (6)
1. the degradation bacterial agent based on bacillus amyloliquefaciens, is characterized in that, described degradation bacterial agent is made up of mixed strains and carrier, and mixed strains accounts for 10% ~ 20% of degradation bacterial agent quality; Described mixed strains comprises bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis, wherein, the mass ratio of bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is 2 ~ 4:1:1:1:1:1:1.
2. the degradation bacterial agent based on bacillus amyloliquefaciens according to claim 1, is characterized in that, described carrier is selected from wheat bran, dregs of beans, wood chip, and the present invention is preferably wheat bran, wood chip.
3. the degradation bacterial agent based on bacillus amyloliquefaciens according to claim 1, it is characterized in that, the mass ratio of described bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is preferably 2:1:1:1:1:1:1.
4. the degradation bacterial agent based on bacillus amyloliquefaciens according to claim 1, it is characterized in that, described bacillus amyloliquefaciens is stored in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) of No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, deposit number: CGMCC No.9196, Classification And Nomenclature: bacillus amyloliquefaciens (
bacillus amyloliquefaciens).
5. the preparation method of the degradation bacterial agent based on bacillus amyloliquefaciens according to claim 1, it is characterized in that, by described bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, subtilis is respectively in LB substratum, 30 DEG C of constant temperature culture 24-36 hour, by bacillus amyloliquefaciens, bacillus cereus, cellulomonas cartae, Alcaligenes, bacillus megaterium, bacillus thuringiensis, the nutrient solution of subtilis mixes according to volume ratio 2 ~ 4:1:1:1:1:1:1, collected by centrifugation thalline, after vacuum lyophilization, with high-temperature sterilization, carrier after oven dry mixes, the mass ratio of mixed strains and carrier is 1 ~ 2:9 ~ 8, be prepared into degradation bacterial agent.
6. an application for the degradation bacterial agent based on bacillus amyloliquefaciens according to claim 1, it is characterized in that, this is applied as, and domestic refuse degradation bacterial agent is applied to the degraded of domestic refuse.
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