CN106591178A - Kitchen garbage degrading composite microbial inoculum and preparation method and application thereof - Google Patents
Kitchen garbage degrading composite microbial inoculum and preparation method and application thereof Download PDFInfo
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- CN106591178A CN106591178A CN201611080349.6A CN201611080349A CN106591178A CN 106591178 A CN106591178 A CN 106591178A CN 201611080349 A CN201611080349 A CN 201611080349A CN 106591178 A CN106591178 A CN 106591178A
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- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 71
- 238000002360 preparation method Methods 0.000 title claims abstract description 25
- 239000002131 composite material Substances 0.000 title claims abstract description 19
- 230000000593 degrading effect Effects 0.000 title claims abstract description 11
- 239000002068 microbial inoculum Substances 0.000 title abstract description 14
- 230000015556 catabolic process Effects 0.000 claims abstract description 76
- 238000006731 degradation reaction Methods 0.000 claims abstract description 76
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 57
- 230000005855 radiation Effects 0.000 claims abstract description 42
- 241000218904 Pseudomonas oryzihabitans Species 0.000 claims abstract description 39
- 241001147698 Staphylococcus cohnii Species 0.000 claims abstract description 37
- 241000589323 Methylobacterium Species 0.000 claims abstract description 36
- 241000588919 Citrobacter freundii Species 0.000 claims abstract description 31
- 238000002156 mixing Methods 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims description 103
- 230000001580 bacterial effect Effects 0.000 claims description 74
- 239000003795 chemical substances by application Substances 0.000 claims description 67
- 239000007788 liquid Substances 0.000 claims description 63
- 238000010411 cooking Methods 0.000 claims description 54
- 244000005700 microbiome Species 0.000 claims description 40
- 238000000855 fermentation Methods 0.000 claims description 30
- 230000004151 fermentation Effects 0.000 claims description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 239000006185 dispersion Substances 0.000 claims description 27
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 26
- 238000012258 culturing Methods 0.000 claims description 26
- 239000000203 mixture Substances 0.000 claims description 25
- 239000001963 growth medium Substances 0.000 claims description 24
- 239000002904 solvent Substances 0.000 claims description 22
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 21
- 238000011534 incubation Methods 0.000 claims description 18
- 238000000034 method Methods 0.000 claims description 16
- 239000011780 sodium chloride Substances 0.000 claims description 13
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000001888 Peptone Substances 0.000 claims description 10
- 108010080698 Peptones Proteins 0.000 claims description 8
- 235000019319 peptone Nutrition 0.000 claims description 8
- 235000015099 wheat brans Nutrition 0.000 claims description 8
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 7
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 7
- 238000012549 training Methods 0.000 claims description 7
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 6
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 6
- 239000005018 casein Substances 0.000 claims description 6
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 6
- 235000021240 caseins Nutrition 0.000 claims description 6
- 239000006071 cream Substances 0.000 claims description 6
- 239000000706 filtrate Substances 0.000 claims description 6
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 claims description 6
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 6
- 235000012015 potatoes Nutrition 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 6
- 210000000582 semen Anatomy 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 3
- 235000007164 Oryza sativa Nutrition 0.000 claims description 3
- 229920002472 Starch Polymers 0.000 claims description 3
- 235000009566 rice Nutrition 0.000 claims description 3
- 239000008107 starch Substances 0.000 claims description 3
- 235000019698 starch Nutrition 0.000 claims description 3
- 241001478240 Coccus Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 claims 1
- 235000009754 Vitis X bourquina Nutrition 0.000 claims 1
- 235000012333 Vitis X labruscana Nutrition 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- 235000014787 Vitis vinifera Nutrition 0.000 claims 1
- 229960005486 vaccine Drugs 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 4
- 239000002054 inoculum Substances 0.000 abstract description 4
- 235000013339 cereals Nutrition 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 235000013311 vegetables Nutrition 0.000 abstract description 3
- 241000251468 Actinopterygii Species 0.000 abstract description 2
- 231100000614 poison Toxicity 0.000 abstract description 2
- 230000007096 poisonous effect Effects 0.000 abstract description 2
- 235000013594 poultry meat Nutrition 0.000 abstract description 2
- 241000520272 Pantoea Species 0.000 abstract 2
- 239000003344 environmental pollutant Substances 0.000 abstract 1
- 235000019688 fish Nutrition 0.000 abstract 1
- 231100000719 pollutant Toxicity 0.000 abstract 1
- 239000001913 cellulose Substances 0.000 description 8
- 229920002678 cellulose Polymers 0.000 description 8
- 239000012531 culture fluid Substances 0.000 description 7
- IQFVPQOLBLOTPF-HKXUKFGYSA-L congo red Chemical compound [Na+].[Na+].C1=CC=CC2=C(N)C(/N=N/C3=CC=C(C=C3)C3=CC=C(C=C3)/N=N/C3=C(C4=CC=CC=C4C(=C3)S([O-])(=O)=O)N)=CC(S([O-])(=O)=O)=C21 IQFVPQOLBLOTPF-HKXUKFGYSA-L 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 241000675208 Paenibacillus cineris Species 0.000 description 5
- 244000052616 bacterial pathogen Species 0.000 description 5
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000012876 carrier material Substances 0.000 description 4
- 239000010794 food waste Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 230000000813 microbial effect Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 230000002159 abnormal effect Effects 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000000796 flavoring agent Substances 0.000 description 3
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- 240000005007 Actinomucor elegans Species 0.000 description 2
- 235000013650 Actinomucor elegans Nutrition 0.000 description 2
- 108010010803 Gelatin Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 235000015278 beef Nutrition 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000004567 concrete Substances 0.000 description 2
- 239000003085 diluting agent Substances 0.000 description 2
- 230000008030 elimination Effects 0.000 description 2
- 238000003379 elimination reaction Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 239000008273 gelatin Substances 0.000 description 2
- 229920000159 gelatin Polymers 0.000 description 2
- 235000019322 gelatine Nutrition 0.000 description 2
- 235000011852 gelatine desserts Nutrition 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- LPUQAYUQRXPFSQ-DFWYDOINSA-M monosodium L-glutamate Chemical compound [Na+].[O-]C(=O)[C@@H](N)CCC(O)=O LPUQAYUQRXPFSQ-DFWYDOINSA-M 0.000 description 2
- 235000013923 monosodium glutamate Nutrition 0.000 description 2
- 239000004223 monosodium glutamate Substances 0.000 description 2
- 238000012797 qualification Methods 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- HGUFODBRKLSHSI-UHFFFAOYSA-N 2,3,7,8-tetrachloro-dibenzo-p-dioxin Chemical compound O1C2=CC(Cl)=C(Cl)C=C2OC2=C1C=C(Cl)C(Cl)=C2 HGUFODBRKLSHSI-UHFFFAOYSA-N 0.000 description 1
- 241001037822 Bacillus bacterium Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 238000013475 authorization Methods 0.000 description 1
- 238000006065 biodegradation reaction Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001722 carbon compounds Chemical class 0.000 description 1
- 235000010980 cellulose Nutrition 0.000 description 1
- 238000005660 chlorination reaction Methods 0.000 description 1
- 238000009264 composting Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000017854 proteolysis Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
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- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Medicinal Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.
Description
Technical field
The invention belongs to biological technical field, is related to a kind of rubbish from cooking complex microorganism degradation bacterial agent and preparation method thereof
With application.
Background technology
In recent years, China is in the quick phase of Urbanization Progress, and urban population quantity is all existed with higher speed every year
Increase.And with the aggregation of population, the discharge capacity of municipal refuse is more and more big, wherein, the ratio shared by changing food waste by
Year rises.Due to containing larger molecular organics matter and the moisture such as substantial amounts of starch, cellulose, protein, oils and fatss in changing food waste,
So which provides good living environment for various microorganisms, and then cause a large amount of of pathogenic bacteria, mosquito and other harmful organisms
Breeding, this to people health and urban environment it is very harmful.Although rubbish from cooking has higher value, and which is aqueous
Rate is high, and calorific value is relatively low, and transport and burning disposal are collected in inconvenience, if dealing with improperly, it is easy to produce the secondary pollutions such as Leachate site
And it is perishable and produce stench.
At present, the method for processing such rubbish all over the world has following several：
1st, landfill method：By refuse collection, it is shipped to landfill yard and paves compacting.Its advantage is for simple and easy to do, and shortcoming is
Subsoil water, air, ambient contamination are easily caused, and takes a large amount of soils.
2nd, burning method：Solid waste high temperature decomposes, the integrated disposal processing of deep oxidation.Its advantage is for rapidly significantly
Garbage volume is reduced, noxious bacteria is eliminated, moreover it is possible to is generated electricity for heat supply, and shortcoming is big for investment, to airborne release nuisance
Matter (such as dioxin etc.) simultaneously spreads bad dust, and generated energy is limited.
3rd, composting process：Become harmless humus after rubbish fermentation.But fermentation period is long, nutrient content is low, body after process
Product is big, is only applicable to ungetable rural area.
Therefore, innoxious, resource is carried out to rubbish from cooking and minimizing processes in short supply to the current per capita resources of China
Present situation is significant.And biodegradation or bioconversion are carried out using biotechnology, not only can effective process city rubbish
Rubbish, and the recycling of resource can be realized.Therefore, compared with Physical, chemical method, biologic treating technique is because with environmental protection peace
The advantages of overall height is imitated and be increasingly becoming the main development direction of following garbage disposal.
Authorization Notice No. discloses a kind of rubbish from cooking degraded elimination type microbial bacteria for the patent of 102010832 B of CN
Agent II, microbial inoculum II are 3.0 × 108～1.5 × 1011The Karl Jaspers (Actinomucor elegans) of cfu/ml
CGMCC No.3881 and 3.0 × 108～1.3 × 1011The Paenibacillus cineris CGMCC No.3883 of cfu/ml
Composite fluid microbial inoculum.Preparation method specifically includes following steps：
1), slant culture：
Karl Jaspers (Actinomucor elegans) CGMCC No.3881 are inoculated in into PDA inclined-planes, will
Paenibacillus cineris CGMCC No.3883 are inoculated in beef extract-peptone inclined-plane, cultivate respectively at 28～30 DEG C
24～72h, carries out the activation of bacterial strain；
2), primary seed solution culture：
By step 1) obtained by activation after Karl Jaspers be inoculated in PDA liquid medium, after the activation of gained
Paenibacillus cineris are inoculated in beef extract-peptone fluid medium, respectively at 28～30 DEG C, 100～250r/
Min shaking table 48～120h of shaken cultivation；The primary seed solution and Paenibacilluscineris of Karl Jaspers are obtained respectively
Primary seed solution；
3), bacterial liquid fermentation culture：
Culture fluid I is placed in the fermentation tank, by step 2) obtained by Paenibacillus cineris primary seed solution
Inoculum concentration according to 5～25% volume ratios of culture fluid I are accounted for is inoculated in culture fluid I, in 30 DEG C～32 DEG C, 30～100r/min
48～72h of fermentation culture；Obtain bacterial solution；
The culture fluid I is：Monosodium glutamate waste liquid is diluted into 10～200 times, and adds adjusting PH with base to 7.1～7.3；
4), fungi liquid fermentation culture：
Culture fluid II is placed in the fermentation tank, by step 2) obtained by Karl Jaspers primary seed solution according to accounting for training
The inoculum concentration of nutrient solution II5～25% volume ratio is inoculated in culture fluid II, in 28～30 DEG C, 30～100r/min fermentation culture 48
～72h；Obtain funguses bacterium solution；
The culture fluid II is：Monosodium glutamate waste liquid is diluted into 10～200 times, and adds adjusting PH with base to 5.4～5.6；
5), by step 3) obtained by bacterial solution and step 4) obtained by funguses bacterium solution mixed, obtain rubbish from cooking
Degraded elimination type microbial bacterial agent II.
Compared to above-mentioned patent, the method have the characteristics that bacterial strain uses therefor is antibacterial, without funguses, easily cultivate.And
The bacteria growth of present invention effect is fast, and fermentation time is short, only 24～48h.Complex microorganism degraded obtained in of the invention
Microbial inoculum can the most rubbish from cooking of fast degradation at short notice, do not produce abnormal flavour, and can once put into and be used for multiple times.
The content of the invention
The purpose of the present invention is exactly to provide one kind and effectively can degrade often to overcome the defect of above-mentioned prior art presence
The rubbishes from cooking such as the vegetable that sees, grain, the flesh of fish, poultry meat, degradation rate are high, produce that abnormal flavour is little, the food waste that nonhazardouss material is produced
Rubbish complex microorganism degradation bacterial agent and preparation method and application.
The purpose of the present invention can be achieved through the following technical solutions：
A kind of rubbish from cooking complex microorganism degradation bacterial agent, the degradation bacterial agent are by bacillus amyloliquefaciens, radiation hardness first
Base shaft bacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, citrobacter freundii, the mixed bacteria of staphylococcus cohnii composition and carrier
Mix, wherein, described mixed bacteria accounts for the 15～25% of degradation bacterial agent gross mass, the matter of water in described degradation bacterial agent
Amount percentage composition is 10～20%, and the pH of degradation bacterial agent is 5.5～8.5, and the viable count that every gram of degradation bacterial agent contains is 106～109
It is individual.
Described bacillus amyloliquefaciens, radiation hardness Methylobacterium, disperse general bacterium, Pseudomonas oryzihabitans, Freund citric acid
The mass ratio of bacillus and staphylococcus cohnii is 1.5～3:1～1.5:1～1.5:1～1.5:1～1.5:1～1.5.
Described bacillus amyloliquefaciens are preserved in Wuhan City, Hubei Province Wuchang District Luo Jia Shan (Bayi Road 299) China allusion quotation
Type culture collection (CCTCC), preservation date are on December 12nd, 2014, and deposit number is CCTCC:AB2014337, point
Class is named as bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
Described bacillus amyloliquefaciens are sieved from the woods soil for piling up corrupt fallen leaves all the year round, concrete to screen and reflect
Determining step is：
(1) take sieved soil 1g sterilized water and dilute 104 times；
(2) 100 μ L diluents are taken and coats aseptic Congo red cellulose culture medium, 28 DEG C of culture 36h；
(3) in the above-mentioned culture medium of picking, the larger bacterium colony of transparent circle carries out purification culture, and preserves in -80 DEG C；
(4) above-mentioned bacterial strain after purification is delivered to Shanghai Shanghai Major Biological Medical Technology Co., Ltd. to be identified.
The aperture of the standard screen used by step (1) is 200mm.
Congo red cellulose culture medium used by step (2), solvent are water, and pH is nature pH, and solute and concentration are：Crystallite
Cellulose 14g/L, magnesium sulfate 0.25g/L, dipotassium hydrogen phosphate 0.5g/L, gelatin 2g/L, Congo red 0.2g/L, agar 14g/L.
The bacterium colony selected in step (3) its transparent loop diameter is all higher than 6mm.
The pure medium that purification culture described in step (3) is adopted, solvent is water, and pH is nature pH, solute and dense
Spend and be：Peptone 10g/L, yeast extract 5g/L, Sodium Chloride 10g/L, agar 15g/L.
The qualification result of step (4) is bacillus amyloliquefaciens, and is preserved in Wuhan City, Hubei Province on December 12nd, 2014
City Wuchang District Luo Jia Shan (Bayi Road 299) China typical culture collection center.
A kind of preparation method of rubbish from cooking complex microorganism degradation bacterial agent, the method specifically include following steps：
(1) respectively to bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund Fructus Citri Limoniae
Acidfast bacilli, staphylococcus cohnii carry out independent strain fermentation culture, obtain bacillus amyloliquefaciens zymocyte liquid, radiation hardness methyl
Bacillus fermentation bacterium solution, the general bacterium zymocyte liquid of dispersion, Pseudomonas oryzihabitans zymocyte liquid, citrobacter freundii zymocyte liquid, section
Family name's Staphylococcal fermentation bacterium solution；
(2) each strain fermentation bacterium solution for taking following parts by weight is mixed, and obtains composite bacteria liquid：Bacillus amyloliquefaciens
15～30 parts of zymocyte liquid, 10～15 parts of radiation hardness Methylobacterium zymocyte liquid disperse 10～15 parts of general bacterium zymocyte liquid, rice skin
10～15 parts of pseudomonass zymocyte liquid, 10～15 parts of citrobacter freundii zymocyte liquid and staphylococcus cohnii zymocyte liquid
10～15 parts；
(3) dry sawdust and wheat bran are taken, is 1 by volume:3～5 mixing, are obtained carrier；
(4) it is 1 in mass ratio by the composite bacteria liquid of step (2) and carrier obtained in step (3):25～30 mix homogeneously,
Described rubbish from cooking complex microorganism degradation bacterial agent is obtained.
For to bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion in step (1)
The culture medium of strain culturing is carried out, solvent is water, and solute and concentration are：3～10g/L of Carnis Bovis seu Bubali cream, 3～10g/L of peptone, chlorination
2～7g/L of sodium, 2～7g/L of dipotassium hydrogen phosphate, 2～5g/L of potassium dihydrogen phosphate, 0.1～1g/L of Magnesium sulfate heptahydrate, ammonium sulfate 1～
1.5g/L。
For Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing in step (1), solvent is water, and pH is nature pH,
Solute and concentration are：Rhizoma Solani tuber osi 200～300g/L of liquor, 15～20g/L of glucose；
Wherein, the preparation method of described Rhizoma Solani tuber osi liquor is：200～300g of peeled potatoes is taken, is thinly sliced, claimed
Then weight, heated and boiled 30min filtered through gauze retain filtrate.
The culture medium of strain culturing is carried out in step (1) for staphylococcus cohnii, solvent is water, and pH is 7.0～7.6,
Solute and concentration are：5～15g/L of casein, 5～10g/L of Semen sojae atricolor powder, 15～20g/L of glucose, 5～10g/L of Sodium Chloride.
It is general to described bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, dispersion in step (1)
Bacterium carries out the condition of culture of spawn culture：25～35 DEG C of temperature, 180～220r/min of rotating speed, 24～48h of incubation time.
Step in (1) to the condition of culture that described Pseudomonas oryzihabitans carry out strain culturing is：30～35 DEG C of temperature, shakes
Bottle shaking table culture, 200～220r/min of rotating speed, 24～36h of incubation time；
To the condition of culture that described staphylococcus cohnii carries out strain culturing it is：28～32 DEG C of temperature, the training of shaking flask shaking table
Support, 180～220r/min of rotating speed, 24～36h of incubation time.
In step (3), in described wheat bran and sawdust, the mass content of moisture is less than 10%.
A kind of application of rubbish from cooking complex microorganism degradation bacterial agent, described complex microorganism degradation bacterial agent are used to degrade
Rubbish from cooking.
In practical application, 0.5～2kg rubbishes from cooking and complex microorganism degradation bacterial agent obtained above are together put into
Into garbage disposer, uniform stirring, at 50～60 DEG C, degradation time is 24～36h to temperature control.
In the present invention, described radiation hardness Methylobacterium, disperse general bacterium, Pseudomonas oryzihabitans, citrobacter freundii and
Staphylococcus cohnii is selected from commercially available industrialization commercialization strain.
Complex microorganism degradation bacterial agent obtained in of the invention can Reusability, that is, treat that the rubbish from cooking of last input is abundant
After degraded, new pending rubbish from cooking is added to proceed degraded again.
The major function of bacillus amyloliquefaciens of the present invention is protein degradation matter and starch, radiation hardness methyl bar
The major function of bacterium is fatty and many carbon compounds of degrading, and the major function for disperseing general bacterium is degraded macromole glucide, and
The major function of Pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii is degraded cellulose.The present invention is adopted
Each bacterial strain degrade rubbish from cooking when each other be synergistic function, i.e., in the case of identical bacteria concentration, six kinds of bacterium
The mix bacterium agent degradation effect of strain is better than single culture microbial inoculum or other several mix bacterium agents.
Compared with prior art, the present invention will be bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, rice skin false
Zymomonas mobiliss, citrobacter freundii, staphylococcus cohnii carry out liquid culture, and the bacterium solution of each bacterial strain is mixed composite microbial
Thing bacterium solution, then mix with wheat bran, sawdust carrier material, rubbish from cooking degradation bacterial agent is obtained, is had the characteristics that：
1) there are various strains with different degradation functions in degradation bacterial agent, can be to containing various things such as vegetable, grain, meat
The rubbish from cooking of matter is effectively degraded；
2) microbial activity in degradation bacterial agent is high, and degradation rate reaches more than 80%, and degradation rate is fast, is processing food waste rubbish
During rubbish, consumption is few, and generation abnormal flavour is little, and low cost is easy to use, does not produce poisonous and harmful substance, safe and reliable environmental protection.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
The preparation method of the present embodiment rubbish from cooking complex microorganism degradation bacterial agent, specifically includes following steps：
1. the preparation of complex microorganism bacterium solution
(1) bacterial strain training is carried out to bacillus amyloliquefaciens, Fo Shi citric acid bacillus, radiation hardness Methylobacterium and the general bacterium of dispersion
Foster culture medium, solvent are water, and solute and concentration are as follows：Carnis Bovis seu Bubali cream 8g/L, peptone 5g/L, Sodium Chloride 5g/L, phosphoric acid hydrogen two
Potassium 5g/L, potassium dihydrogen phosphate 5g/L, Magnesium sulfate heptahydrate 1g/L, ammonium sulfate 1g/L, pH are 7.2.
Condition of culture is：28 DEG C of temperature, rotating speed 200r/min, incubation time 20h.
Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing, solvent is water, and solute and concentration are as follows：Rhizoma Solani tuber osi
200g/L, glucose 15g/L, pH are 7.5.Wherein Rhizoma Solani tuber osi liquor method is as follows：Peeled potatoes 200g is taken, is thinly sliced,
Weigh, then heated and boiled 25min filtered through gauze retains filtrate.
Condition of culture is：30 DEG C of temperature, rotating speed 220r/min, incubation time 24h.
(3) culture medium of strain culturing is carried out to staphylococcus cohnii, solvent is water, and solute and concentration are as follows：Casein
10g/L, Semen sojae atricolor powder 10g/L, glucose 15g/L, Sodium Chloride 5g/L, pH are 7.0.
Condition of culture is：28 DEG C of temperature, rotating speed 220r/min, incubation time 28h.
(4) bacillus amyloliquefaciens bacterium solution 90mL is taken, radiation hardness Methylobacterium bacterium solution 45mL disperses general bacterium bacterium solution 45mL,
Pseudomonas oryzihabitans bacterium solution 45mL, Fo Shi citric acid bacillus bacterium solutions 45mL, staphylococcus cohnii bacterium solution 30mL mix homogeneously are obtained
Complex microorganism bacterium solution.
2. the preparation of carrier material
Take 3L wheat brans and 15L sawdusts, the carrier material of mix homogeneously.
The preparation of degradation bacterial agent
Take complex microorganism bacterium solution 300mL in 1 to mix homogeneously with the carrier material of 12L in 2, rubbish from cooking degradation bacteria is obtained
Agent.
Take 12L rubbish from cooking degradation bacterial agents, pour in rubbish stirring datatron, degradation bacterial agent top add 0.6～
1.2kg rubbishes from cooking, cover datatron lid and form enclosed environment, and heated and stirred temperature control is at 50 DEG C.Per 24 hours to rubbish
The gross weight of rubbish datatron is once weighed, and calculates degradation efficiency.
Degradation efficiency reduces efficiency to embody with weight：
Degradation efficiency=(B-C)/A × 100%
A:Input rubbish from cooking weight (kg) B：Before processing gross weight (kg) C：Gross weight (kg) after process.
Experimental result is shown in Table 1.
1 degradation bacterial agent of table to rubbish from cooking degradation efficiency over time
Knowable to table 1 is analyzed, degradation bacterial agent of the present invention can quickly reduce the weight and effect of rubbish from cooking at short notice
Fruit is good, provides theoretical basiss and experimental basis to process rubbish from cooking with biotechnology.
Embodiment 2
The present embodiment rubbish from cooking complex microorganism degradation bacterial agent be by bacillus amyloliquefaciens, radiation hardness Methylobacterium,
Disperse general bacterium, Pseudomonas oryzihabitans, citrobacter freundii, staphylococcus cohnii composition mixed bacteria mix with carrier and
Into, wherein, mixed bacteria accounts for the 15% of degradation bacterial agent gross mass, and in degradation bacterial agent, the weight/mass percentage composition of water is 20%, degraded
The pH of microbial inoculum is 5.5, and the viable count that every gram of degradation bacterial agent contains is 106It is individual.
Wherein, bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund citric acid bar
The mass ratio of bacterium and staphylococcus cohnii is 1.5:1:1:1:1:1.
The bacillus amyloliquefaciens adopted by the present embodiment are preserved in Wuhan City, Hubei Province Wuchang District Luo Jia Shan (Bayi Road
No. 299) China typical culture collection center (CCTCC), preservation date is on December 12nd, 2014.Deposit number is CCTCC:
AB2014337, Classification And Nomenclature are bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
In the present embodiment, bacillus amyloliquefaciens are sieved from the woods soil for piling up corrupt fallen leaves all the year round, concrete to screen
And authentication step is：
(1) take sieved soil 1g sterilized water and dilute 104 times；
(2) 100 μ L diluents are taken and coats aseptic Congo red cellulose culture medium, 28 DEG C of culture 36h；
(3) in the above-mentioned culture medium of picking, the larger bacterium colony of transparent circle carries out purification culture, and preserves in -80 DEG C；
(4) above-mentioned bacterial strain after purification is delivered to Shanghai Shanghai Major Biological Medical Technology Co., Ltd. to be identified.
The aperture of the standard screen used by step (1) is 200mm.
Congo red cellulose culture medium used by step (2), solvent are water, and pH is nature pH, and solute and concentration are：Crystallite
Cellulose 14g/L, magnesium sulfate 0.25g/L, dipotassium hydrogen phosphate 0.5g/L, gelatin 2g/L, Congo red 0.2g/L, agar 14g/L.
The bacterium colony selected in step (3) its transparent loop diameter is all higher than 6mm.
The pure medium that purification culture described in step (3) is adopted, solvent is water, and pH is nature pH, solute and dense
Spend and be：Peptone 10g/L, yeast extract 5g/L, Sodium Chloride 10g/L, agar 15g/L.
The qualification result of step (4) is bacillus amyloliquefaciens, and is preserved in Wuhan City, Hubei Province on December 12nd, 2014
City Wuchang District Luo Jia Shan (Bayi Road 299) China typical culture collection center.
The preparation method of the present embodiment rubbish from cooking complex microorganism degradation bacterial agent, specifically includes following steps：
(1) respectively to bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund Fructus Citri Limoniae
Acidfast bacilli, staphylococcus cohnii carry out independent strain fermentation culture, obtain bacillus amyloliquefaciens zymocyte liquid, radiation hardness methyl
Bacillus fermentation bacterium solution, the general bacterium zymocyte liquid of dispersion, Pseudomonas oryzihabitans zymocyte liquid, citrobacter freundii zymocyte liquid, section
Family name's Staphylococcal fermentation bacterium solution；
(2) each strain fermentation bacterium solution for taking following parts by weight is mixed, and obtains composite bacteria liquid：Bacillus amyloliquefaciens
15 parts of zymocyte liquid, 10 parts of radiation hardness Methylobacterium zymocyte liquid disperse 10 parts of general bacterium zymocyte liquid, Pseudomonas oryzihabitans fermentation
10 parts of 10 parts of bacterium solution, 10 parts of citrobacter freundii zymocyte liquid and staphylococcus cohnii zymocyte liquid；
(3) dry sawdust and wheat bran are taken, is 1 by volume:3 mixing, are obtained carrier；
(4) it is 1 by volume by the composite bacteria liquid of step (2) and carrier obtained in step (3):25 mix homogeneously, that is, make
Obtain rubbish from cooking complex microorganism degradation bacterial agent.
For to bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion in step (1)
The culture medium of strain culturing is carried out, solvent is water, and solute and concentration are：Carnis Bovis seu Bubali cream 3g/L, peptone 3g/L, Sodium Chloride 2g/L,
Dipotassium hydrogen phosphate 2g/L, potassium dihydrogen phosphate 2g/L, Magnesium sulfate heptahydrate 0.1g/L, ammonium sulfate 1g/L.
For Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing in step (1), solvent is water, and pH is nature pH,
Solute and concentration are：Rhizoma Solani tuber osi liquor 200g/L, glucose 15g/L；
Wherein, the preparation method of Rhizoma Solani tuber osi liquor is：Peeled potatoes 200g is taken, is thinly sliced, weighed, heated and boiled
30min, then filtered through gauze retains filtrate.
The culture medium of strain culturing is carried out in step (1) for staphylococcus cohnii, solvent is water, and pH is 7.0, solute and
Concentration is：Casein 5g/L, Semen sojae atricolor powder 5g/L, glucose 15g/L, Sodium Chloride 5g/L.
Bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion are carried out in step (1)
The condition of culture of spawn culture is：25 DEG C of temperature, rotating speed 180r/min, incubation time 48h.
Step in (1) to the condition of culture that Pseudomonas oryzihabitans carry out strain culturing is：30 DEG C of temperature, the training of shaking flask shaking table
Support, rotating speed 200r/min, incubation time 36h；
To the condition of culture that described staphylococcus cohnii carries out strain culturing it is：28 DEG C of temperature, shaking flask shaking table culture turn
Fast 180r/min, incubation time 36h.
Rubbish from cooking complex microorganism degradation bacterial agent obtained in the present embodiment is used for rubbish from cooking of degrading.
Embodiment 3
The present embodiment rubbish from cooking complex microorganism degradation bacterial agent be by bacillus amyloliquefaciens, radiation hardness Methylobacterium,
Disperse general bacterium, Pseudomonas oryzihabitans, citrobacter freundii, staphylococcus cohnii composition mixed bacteria mix with carrier and
Into, wherein, mixed bacteria accounts for the 25% of degradation bacterial agent gross mass, and in degradation bacterial agent, the weight/mass percentage composition of water is 10%, degraded
The pH of microbial inoculum is 8.5, and the viable count that every gram of degradation bacterial agent contains is 109It is individual.
Wherein, bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund citric acid bar
The mass ratio of bacterium and staphylococcus cohnii is 3:1.5:1.5:1.5:1.5:1.
The bacillus amyloliquefaciens adopted by the present embodiment are preserved in Wuhan City, Hubei Province Wuchang District Luo Jia Shan (Bayi Road
No. 299) China typical culture collection center (CCTCC), preservation date is on December 12nd, 2014, and deposit number is CCTCC:
AB2014337, Classification And Nomenclature are bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
The preparation method of the present embodiment rubbish from cooking complex microorganism degradation bacterial agent, specifically includes following steps：
(1) respectively to bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund Fructus Citri Limoniae
Acidfast bacilli, staphylococcus cohnii carry out independent strain fermentation culture, obtain bacillus amyloliquefaciens zymocyte liquid, radiation hardness methyl
Bacillus fermentation bacterium solution, the general bacterium zymocyte liquid of dispersion, Pseudomonas oryzihabitans zymocyte liquid, citrobacter freundii zymocyte liquid, section
Family name's Staphylococcal fermentation bacterium solution；
(2) each strain fermentation bacterium solution for taking following parts by weight is mixed, and obtains composite bacteria liquid：Bacillus amyloliquefaciens
30 parts of zymocyte liquid, 15 parts of radiation hardness Methylobacterium zymocyte liquid disperse 15 parts of general bacterium zymocyte liquid, Pseudomonas oryzihabitans fermentation
10 parts of 15 parts of bacterium solution, 15 parts of citrobacter freundii zymocyte liquid and staphylococcus cohnii zymocyte liquid；
(3) dry sawdust and wheat bran are taken, is 1 by volume:5 mixing, are obtained carrier；
(4) it is 1 by volume by the composite bacteria liquid of step (2) and carrier obtained in step (3):30 mix homogeneously, that is, make
Obtain rubbish from cooking complex microorganism degradation bacterial agent.
For to bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion in step (1)
The culture medium of strain culturing is carried out, solvent is water, and solute and concentration are：Carnis Bovis seu Bubali cream 10g/L, peptone 10g/L, Sodium Chloride 7g/
L, dipotassium hydrogen phosphate 7g/L, potassium dihydrogen phosphate 5g/L, Magnesium sulfate heptahydrate 1g/L, ammonium sulfate 1.5g/L.
For Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing in step (1), solvent is water, and pH is nature pH,
Solute and concentration are：Rhizoma Solani tuber osi liquor 300g/L, glucose 20g/L；
Wherein, the preparation method of Rhizoma Solani tuber osi liquor is：Peeled potatoes 300g is taken, is thinly sliced, weighed, heated and boiled
30min, then filtered through gauze retains filtrate.
The culture medium of strain culturing is carried out in step (1) for staphylococcus cohnii, solvent is water, and pH is 7.6, solute and
Concentration is：Casein 15g/L, Semen sojae atricolor powder 10g/L, glucose 20g/L, Sodium Chloride 10g/L.
Bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion are carried out in step (1)
The condition of culture of spawn culture is：35 DEG C of temperature, rotating speed 220r/min, incubation time 24h.
Step in (1) to the condition of culture that Pseudomonas oryzihabitans carry out strain culturing is：35 DEG C of temperature, the training of shaking flask shaking table
Support, rotating speed 220r/min, incubation time 24h；
To the condition of culture that described staphylococcus cohnii carries out strain culturing it is：32 DEG C of temperature, shaking flask shaking table culture turn
Fast 220r/min, incubation time 24h.
Rubbish from cooking complex microorganism degradation bacterial agent obtained in the present embodiment is used for rubbish from cooking of degrading.
Embodiment 4
The present embodiment rubbish from cooking complex microorganism degradation bacterial agent be by bacillus amyloliquefaciens, radiation hardness Methylobacterium,
Disperse general bacterium, Pseudomonas oryzihabitans, citrobacter freundii, staphylococcus cohnii composition mixed bacteria mix with carrier and
Into, wherein, mixed bacteria accounts for the 20% of degradation bacterial agent gross mass, and in degradation bacterial agent, the weight/mass percentage composition of water is 16%, degraded
The pH of microbial inoculum is 7.5, and the viable count that every gram of degradation bacterial agent contains is 108It is individual.
Wherein, bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund citric acid bar
The mass ratio of bacterium and staphylococcus cohnii is 2:1.2:1.2:1:1.2:1.5.
The bacillus amyloliquefaciens adopted by the present embodiment are preserved in Wuhan City, Hubei Province Wuchang District Luo Jia Shan (Bayi Road
No. 299) China typical culture collection center (CCTCC), preservation date is on December 12nd, 2014, and deposit number is CCTCC:
AB2014337, Classification And Nomenclature are bacillus amyloliquefaciens (Bacillus amyloliquefaciens).
The preparation method of the present embodiment rubbish from cooking complex microorganism degradation bacterial agent, specifically includes following steps：
(1) respectively to bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund Fructus Citri Limoniae
Acidfast bacilli, staphylococcus cohnii carry out independent strain fermentation culture, obtain bacillus amyloliquefaciens zymocyte liquid, radiation hardness methyl
Bacillus fermentation bacterium solution, the general bacterium zymocyte liquid of dispersion, Pseudomonas oryzihabitans zymocyte liquid, citrobacter freundii zymocyte liquid, section
Family name's Staphylococcal fermentation bacterium solution；
(2) each strain fermentation bacterium solution for taking following parts by weight is mixed, and obtains composite bacteria liquid：Bacillus amyloliquefaciens
20 parts of zymocyte liquid, 12 parts of radiation hardness Methylobacterium zymocyte liquid disperse 12 parts of general bacterium zymocyte liquid, Pseudomonas oryzihabitans fermentation
15 parts of 10 parts of bacterium solution, 12 parts of citrobacter freundii zymocyte liquid and staphylococcus cohnii zymocyte liquid；
(3) dry sawdust and wheat bran are taken, is 1 by volume:4 mixing, are obtained carrier；
(4) it is 1 by volume by the composite bacteria liquid of step (2) and carrier obtained in step (3):28 mix homogeneously, that is, make
Obtain rubbish from cooking complex microorganism degradation bacterial agent.
For to bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion in step (1)
The culture medium of strain culturing is carried out, solvent is water, and solute and concentration are：Carnis Bovis seu Bubali cream 7g/L, peptone 7g/L, Sodium Chloride 3g/L,
Dipotassium hydrogen phosphate 4g/L, potassium dihydrogen phosphate 3g/L, Magnesium sulfate heptahydrate 0.8g/L, ammonium sulfate 1.2g/L.
For Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing in step (1), solvent is water, and pH is nature pH,
Solute and concentration are：Rhizoma Solani tuber osi liquor 240g/L, glucose 18g/L；
Wherein, the preparation method of Rhizoma Solani tuber osi liquor is：Peeled potatoes 240g is taken, is thinly sliced, weighed, heated and boiled
30min, then filtered through gauze retains filtrate.
The culture medium of strain culturing is carried out in step (1) for staphylococcus cohnii, solvent is water, and pH is 7.3, solute and
Concentration is：Casein 12g/L, Semen sojae atricolor powder 7g/L, glucose 16g/L, Sodium Chloride 8g/L.
Bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion are carried out in step (1)
The condition of culture of spawn culture is：30 DEG C of temperature, rotating speed 200r/min, incubation time 36h.
Step in (1) to the condition of culture that Pseudomonas oryzihabitans carry out strain culturing is：35 DEG C of temperature, the training of shaking flask shaking table
Support, rotating speed 200r/min, incubation time 28h；
To the condition of culture that described staphylococcus cohnii carries out strain culturing it is：30 DEG C of temperature, shaking flask shaking table culture turn
Fast 200r/min, incubation time 28h.
Rubbish from cooking complex microorganism degradation bacterial agent obtained in the present embodiment is used for rubbish from cooking of degrading.
The above-mentioned description to embodiment is to be understood that for ease of those skilled in the art and use invention.
Person skilled in the art obviously easily can make various modifications to these embodiments, and described herein general
Principle is applied in other embodiment without through performing creative labour.Therefore, the invention is not restricted to above-described embodiment, ability
Field technique personnel announcement of the invention, the improvement made without departing from scope and modification all should be the present invention's
Within protection domain.
Claims (10)
1. a kind of rubbish from cooking complex microorganism degradation bacterial agent, it is characterised in that the degradation bacterial agent be by bacillus amyloliquefaciens,
Radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, citrobacter freundii, the mixed vaccine of staphylococcus cohnii composition
Plant and mix with carrier, wherein, described mixed bacteria accounts for the 15～25% of degradation bacterial agent gross mass, described degradation bacterial agent
The weight/mass percentage composition of middle water is 10～20%, and the pH of degradation bacterial agent is 5.5～8.5, the viable count that every gram of degradation bacterial agent contains
For 106～109It is individual.
2. a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 1, it is characterised in that described Xie Dian
Afnyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii
Mass ratio be 1.5～3:1～1.5:1～1.5:1～1.5:1～1.5:1～1.5.
3. a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 1, it is characterised in that described Xie Dian
Afnyloliquefaciens are preserved in China typical culture collection center, and address is Wuhan City, Hubei Province Wuchang District Luo Jia Shan Bayi Road
No. 299, preservation date is on December 12nd, 2014, and deposit number is CCTCC:AB2014337, Classification And Nomenclature are solution starch spore
Bacillus (Bacillus amyloliquefaciens).
4. a kind of preparation method of the rubbish from cooking complex microorganism degradation bacterial agent as described in any one of claims 1 to 3, which is special
Levy and be, the method specifically includes following steps：
(1) respectively to bacillus amyloliquefaciens, radiation hardness Methylobacterium, the general bacterium of dispersion, Pseudomonas oryzihabitans, Freund citric acid bar
Bacterium, staphylococcus cohnii carry out independent strain fermentation culture, obtain bacillus amyloliquefaciens zymocyte liquid, radiation hardness Methylobacterium
Zymocyte liquid, the general bacterium zymocyte liquid of dispersion, Pseudomonas oryzihabitans zymocyte liquid, citrobacter freundii zymocyte liquid, Coriolis Portugal
Grape coccus zymocyte liquid；
(2) each strain fermentation bacterium solution for taking following parts by weight is mixed, and obtains composite bacteria liquid：Bacillus amyloliquefaciens ferment
15～30 parts of bacterium solution, 10～15 parts of radiation hardness Methylobacterium zymocyte liquid disperse 10～15 parts of general bacterium zymocyte liquid, and rice skin is false single
10～15 parts of born of the same parents bacterium zymocyte liquid, 10～15 parts of citrobacter freundii zymocyte liquid and staphylococcus cohnii zymocyte liquid 10～
15 parts；
(3) dry sawdust and wheat bran are taken, is 1 by volume:3～5 mixing, are obtained carrier；
(4) it is 1 by volume by the composite bacteria liquid of step (2) and carrier obtained in step (3):25～30 mix homogeneously, that is, make
Obtain described rubbish from cooking complex microorganism degradation bacterial agent.
5. the preparation method of a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 4, it is characterised in that
In step (1) for bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, disperse general bacterium to carry out bacterial strain
The culture medium of culture, solvent are water, and solute and concentration are：3～10g/L of Carnis Bovis seu Bubali cream, 3～10g/L of peptone, 2～7g/ of Sodium Chloride
L, 2～7g/L of dipotassium hydrogen phosphate, 2～5g/L of potassium dihydrogen phosphate, 0.1～1g/L of Magnesium sulfate heptahydrate, 1～1.5g/L of ammonium sulfate.
6. the preparation method of a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 4, it is characterised in that
For Pseudomonas oryzihabitans are carried out with the culture medium of strain culturing in step (1), solvent is water, and pH is nature pH, solute and dense
Spend and be：Rhizoma Solani tuber osi 200～300g/L of liquor, 15～20g/L of glucose；
Wherein, the preparation method of described Rhizoma Solani tuber osi liquor is：200～300g of peeled potatoes is taken, is thinly sliced, weighed, plus
Heat boils 30min, then filtered through gauze retains filtrate.
7. the preparation method of a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 4, it is characterised in that
Carry out the culture medium of strain culturing in step (1) for staphylococcus cohnii, solvent is water, and pH is 7.0～7.6, solute and dense
Spend and be：5～15g/L of casein, 5～10g/L of Semen sojae atricolor powder, 15～20g/L of glucose, 5～10g/L of Sodium Chloride.
8. the preparation method of a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 4, it is characterised in that
Bacterium is carried out to described bacillus amyloliquefaciens, citrobacter freundii, radiation hardness Methylobacterium, the general bacterium of dispersion in step (1)
Planting the condition of culture cultivated is：25～35 DEG C of temperature, 180～220r/min of rotating speed, 24～48h of incubation time.
9. the preparation method of a kind of rubbish from cooking complex microorganism degradation bacterial agent according to claim 4, it is characterised in that
Step in (1) to the condition of culture that described Pseudomonas oryzihabitans carry out strain culturing is：30～35 DEG C of temperature, the training of shaking flask shaking table
Support, 200～220r/min of rotating speed, 24～36h of incubation time；
To the condition of culture that described staphylococcus cohnii carries out strain culturing it is：28～32 DEG C of temperature, shaking flask shaking table culture turn
Speed 180～220r/min, 24～36h of incubation time.
10. a kind of application of the rubbish from cooking complex microorganism degradation bacterial agent as described in any one of claims 1 to 3, its feature
It is that described complex microorganism degradation bacterial agent is used for rubbish from cooking of degrading.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN107282593A (en) * | 2017-06-17 | 2017-10-24 | 江苏奔拓电气科技有限公司 | A kind of kitchen garbage degraded specific complex microbial bacterial agent |
| CN107435032A (en) * | 2017-06-30 | 2017-12-05 | 浙江华庆元生物科技有限公司 | A kind of kitchen garbage degraded compound bacteria and its application |
| CN107760616A (en) * | 2017-07-25 | 2018-03-06 | 青岛海芬健康产业科技有限公司 | A kind of microbial bacterial agent for rubbish from cooking of degrading and preparation method thereof |
| CN108902447A (en) * | 2018-06-22 | 2018-11-30 | 苏州净瑞环保科技有限公司 | Disease prevention type fly maggot feeding feed, preparation method and prophylactic fly-maggot feed |
| CN110760460A (en) * | 2019-09-30 | 2020-02-07 | 浙江工业大学 | Compound microbial inoculum capable of efficiently degrading kitchen waste oil components and application thereof |
| CN110894477A (en) * | 2019-09-18 | 2020-03-20 | 浙江工业大学 | Compound microbial inoculum for degrading kitchen waste, application and kitchen waste degradation method |
| CN111286478A (en) * | 2020-03-09 | 2020-06-16 | 西南交通大学 | Grease degradation composite bacterial agent and preparation method and application thereof |
| CN111662853A (en) * | 2020-07-17 | 2020-09-15 | 中国科学院成都生物研究所 | Kitchen waste biological drying stabilizing microbial agent and preparation method and application thereof |
| CN111961606A (en) * | 2020-06-24 | 2020-11-20 | 人与自然环保生物科技(南京)有限公司 | Kitchen waste composite microbial degradation microbial inoculum and preparation method and application thereof |
| CN112195132A (en) * | 2020-10-26 | 2021-01-08 | 中国科学院上海高等研究院 | Methylobacterium thermophilum and application thereof in organic solid waste |
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| CN113652371A (en) * | 2021-08-17 | 2021-11-16 | 湖南泰洁环保科技有限公司 | Ultrahigh-temperature kitchen waste treatment microbial inoculum and preparation method thereof |
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| WO2022038305A1 (en) * | 2020-08-17 | 2022-02-24 | Eino Elias Hakalehto | Microbiological method and equipment for the fractionating and utilization of slaughter house and sauce industry wastes and side streams |
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| CN115216433A (en) * | 2022-09-01 | 2022-10-21 | 浙江永尔佳环保科技有限公司 | Kitchen waste degrading composite bacterium and application thereof |
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| CN112195132A (en) * | 2020-10-26 | 2021-01-08 | 中国科学院上海高等研究院 | Methylobacterium thermophilum and application thereof in organic solid waste |
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| CN113322251B (en) * | 2021-04-09 | 2022-05-10 | 杭州楠大环保科技有限公司 | Composite microbial degradation microbial inoculum for kitchen waste treatment and preparation method and application thereof |
| CN113444657A (en) * | 2021-05-20 | 2021-09-28 | 华东师范大学 | Solid-state microbial composite microbial agent for promoting aerobic composting of kitchen waste and preparation and application thereof |
| CN113652370A (en) * | 2021-08-11 | 2021-11-16 | 桃墨环境技术(上海)有限公司 | Composite microbial inoculant for degrading kitchen garbage and degradation method thereof |
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| CN115140846A (en) * | 2022-07-06 | 2022-10-04 | 江苏聚庚科技有限公司 | Composite treating agent, preparation method and application thereof in wastewater purification |
| CN115216433A (en) * | 2022-09-01 | 2022-10-21 | 浙江永尔佳环保科技有限公司 | Kitchen waste degrading composite bacterium and application thereof |
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