CN107760616A - A kind of microbial bacterial agent for rubbish from cooking of degrading and preparation method thereof - Google Patents
A kind of microbial bacterial agent for rubbish from cooking of degrading and preparation method thereof Download PDFInfo
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- CN107760616A CN107760616A CN201710610446.XA CN201710610446A CN107760616A CN 107760616 A CN107760616 A CN 107760616A CN 201710610446 A CN201710610446 A CN 201710610446A CN 107760616 A CN107760616 A CN 107760616A
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- bacterial agent
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F9/00—Fertilisers from household or town refuse
- C05F9/04—Biological compost
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Abstract
The present invention provides a kind of microbial bacterial agent for rubbish from cooking of degrading and preparation method thereof, and component and its mass ratio are：100 300 parts of bacillus subtilis, 100 150 parts of hot streptomyces diastaticus, 120 150 parts of stearothermophilus spore bud bacillus, 80 120 parts of saccharomyces cerevisiae, 150 300 parts of Lactobacillus plantarum, 200 300 parts of Enteromorpha proteinase.Preparation method includes actication of culture, mixed fermentation, three big step of dry mixed, the degradation speed that the product of the present invention formed can speed up rubbish from cooking is prepared, reduces energy consumption, quick environmental protection, and rubbish from cooking is contributed to preferably to improve crop disease-resistant ability after the processing of fertile powder.
Description
Technical field
The present invention relates to microbial bacterial agent field, more particularly to one kind degraded rubbish from cooking microbial bacterial agent and its
Preparation method.
Background technology
The quickening of urbanization process, the surge of population and growth in the living standard so that people are enjoying abundant material
While with cultural life, substantial amounts of family domestic garbage is also generated, the family domestic garbage of substantial amounts influences all the more people
Quality of life, living environment, paying attention to environmental protection, today of quality of life stressed, for house refuse especially kitchen
Efficient, the environmentally friendly and recycling treatment of remaining rubbish turns into the effective countermeasure solved the problems, such as.
At present, for rubbish from cooking, major way that people take for directly abandon or by refuse treatment plant by its
Directly into blow-off line after smashing, the biologic treating techniques such as garbage disposal mainly takes unified landfill, burned, efficiency is poor,
Reuse ratio is relatively low after processing, is unfavorable for green, especially rubbish from cooking, lacks targetedly special disposal, fails
The material such as carbohydrate, protein, starch, cellulose in rubbish from cooking is recycled.
Plant rennet, which has to reduce, to be consumed energy, and the excellent results such as quick environmental protection, its application is also increasingly closed by people
Note.And the phyteral that wherein Enteromorpha proteinase uses is Enteromorpha, its raw material is easily obtained, cheap, prepares culture not by bar
Part limits, due to Enteromorpha growth characteristics, using a kind of Enteromorpha of my company granted patent Application No. 201310496220.3
The preparation method of protease, the Enteromorpha proteinase of preparation, enzyme activity is higher with purity, and the units of enteromorpha protein enzyme activity ＞ 2,300,000/
Gram, be advantageous to shorten course of reaction, accelerate the degradation speed of rubbish from cooking, reduce energy consumption, quick environmentally friendly, the waterside in course of reaction
Tongue polysaccharide can help rubbish from cooking preferably to improve crop disease-resistant ability after the processing of fertile powder.
The content of the invention
It is an object of the invention to solve background section above by one kind degraded rubbish from cooking microbial bacterial agent
The problem of mentioning, by the good synergy of each bacterial strain in microbial inoculum, rubbish from cooking of effectively degrading, organic fertilizer powder is converted into,
Reduce caused peculiar smell in garbage processing procedure.
To achieve the above object, the present invention uses following technical scheme：
The present invention relates to a kind of microbial bacterial agent, by bacillus subtilis, hot streptomyces diastaticus, stearothermophilus spore bud bacillus,
Lactobacillus acidophilus, saccharomyces cerevisiae, Enteromorpha proteinase composition.
The microbial bacterial agent, component and its mass ratio are：Bacillus subtilis 100-300 parts, hot streptomyces diastaticus
100-150 parts, stearothermophilus spore bud bacillus 120-150 parts, saccharomyces cerevisiae 80-120 parts, Lactobacillus plantarum 150-300 parts, Enteromorpha
Protease 200-300 parts.
The bacillus subtilis is that China General Microbiological culture presevation administrative center deposit number is（CGMCC
NO.7088）, the hot streptomyces diastaticus be China General Microbiological culture presevation administrative center preservation, numbering is
（CGMCC4.1759）, the stearothermophilus spore bud bacillus be China General Microbiological culture presevation administrative center preservation, numbering is
（CGMCC1.1145）, the saccharomyces cerevisiae be China General Microbiological culture presevation administrative center preservation, numbering is
(CGMCC2.3868), the Lactobacillus plantarum is China General Microbiological culture presevation administrative center preservation, and numbering is
（CGMCC1.6971 ）, bacterial strain described above can be that market sale is any.
The preparation method of the microbial bacterial agent is as follows：
Step 1, actication of culture：Each strain is inoculated with corresponding culture medium by the ratio, wherein,
The bacillus subtilis activation medium uses beef-protein medium, is formulated as beef extract 0.3g/L, peptone
1.0g/L, sodium chloride 0.5g/L, sodium hydroxide 0.5g/L, cultivation temperature are 35-40 DEG C；
The saccharomyces cerevisiae activation medium uses brewer's wort potato sucrose culture medium, is formulated as the g/L of dehydrated potato powder 7.0, sugarcane
20.0 g/L of sugar, cultivation temperature are 30-35 DEG C；
The stearothermophilus spore bud baccilus medium uses glucase peptone water medium, is formulated as peptone
5.0g/L, glucose 0.25g/L, bromocresol purple 0.010g/L, cultivation temperature are 50-52 DEG C；
The hot streptomyces diastaticus culture medium uses yeast extract malt extract culture medium, is formulated as yeast extract 2.0g/L, malt extract
5.0 g/L, glucose 2.0g/L, cultivation temperature are 30-35 DEG C；
The Lactobacillus plantarum activation medium uses lactic acid bacteria culture medium, is formulated as albumen 5.0g/L, beef extract 5.0g/
L, yeast extract 2.5g/L, glucose 10.0g/L, Tween 80 0.5g/L, dipotassium hydrogen phosphate 1.0g/L, the g/L of sodium acetate 2.5, lemon
The sour g/L of two ammonium 1, epsom salt 0.025g/L, cultivation temperature are 20-35 DEG C.
Step 2, mixed fermentation：By the good bacillus subtilis of activation culture, hot streptomyces diastaticus, stearothermophilus spore
Bud bacillus, saccharomyces cerevisiae, Lactobacillus plantarum are mixed in the ratio, are inoculated into fermentation tank, volume is shared by fermentation medium
60%, inoculation volume is 3%, uses fermentation medium as beancake powder 5-20g/L, corn steep liquor 5-15g/L, urea 5-10g/L, sulfuric acid
Magnesium 0.1-0.5g/L, calcium carbonate 1-5g/L, sodium sulphate 0.1-0.5 g/L, glucose 15-20 g/L, 30-45 DEG C of cultivation temperature,
After high density stirring fermentation, mixed bacteria liquid is obtained.
Step 3, dry mixed：The mixed bacteria liquid of atomization is made by dried microorganism bacterium powder using the method for spray drying,
Dried microorganism bacterium powder is stirred with enteromorpha protein enzyme crystal again by proportion of composing after mixing, formed of the present invention micro-
Bacteria agent.
The various microorganism fungus kinds of the present invention have completed antagonistic experiment before carrying out mixed fermentation, it was demonstrated that are not present between strain
Antagonism, the synergy of various microorganism fungus kinds can be realized.
Embodiment
Below in conjunction with specific embodiment, it is further elaborated for the present invention.Illustrated for embodiment, it should be appreciated that these
Embodiment is merely to illustrate the present invention, does not limit the scope of the invention, those skilled in the art institute on the basis of present invention
The various changes carried out and modification, also belong to the application appended claims and limit scope.
Embodiment 1
A kind of microbial bacterial agent, the component of sample 1 and its mass ratio are：200 parts of bacillus subtilis, hot streptomyces diastaticus
120 parts, 120 parts of stearothermophilus spore bud bacillus, 100 parts of saccharomyces cerevisiae, 150 parts of Lactobacillus plantarum；
The component of sample 2 and its mass ratio are：200 parts of bacillus subtilis, 120 parts of hot streptomyces diastaticus, stearothermophilus spore bud
120 parts of bacillus, 100 parts of saccharomyces cerevisiae, 150 parts of Lactobacillus plantarum, 250 parts of Enteromorpha proteinase；
Rubbish from cooking 100kg is put into, puts into the microbial bacterial agent, input amount is the 8% of rubbish from cooking weight, through more sub-samplings
Observation obtains data below：
Wherein, rubbish from cooking degradation rate=（Weight after input rubbish from cooking weight+input microbial bacterial agent weight-processing）/ input
Rubbish from cooking weight X100%.Enteromorpha proteinase is added in the present invention can play acceleration reaction, improve the effect of degradation rate.
Embodiment 2, application of the microbial inoculum in family expenses kitchen refuse processing apparatus：
From rubbish from cooking caused by family, after being smashed by Domestic garbage-treatment device completion drying, by rubbish from cooking weight
The 8% input microbial bacterial agent, choose microbial bacterial agent component and its mass ratio be：250 parts of bacillus subtilis, heat are formed sediment
120 parts of powder enzyme streptomycete, 120 parts of stearothermophilus spore bud bacillus, 120 parts of saccharomyces cerevisiae, 150 parts of Lactobacillus plantarum, enteromorpha protein
250 parts of enzyme, after being stirred again, after fermenting 24 hours, measure, wherein input rubbish from cooking weight is 2kg, after processing
Rubbish from cooking weight is 0.22kg, is computed, and using the present invention, rubbish from cooking degradation rate is 98%.
Claims (4)
1. a kind of microbial bacterial agent for rubbish from cooking of degrading, it is characterised in that component and its mass ratio are：Bacillus subtilis
100-300 parts, hot streptomyces diastaticus 100-150 parts, stearothermophilus spore bud bacillus 120-150 parts, saccharomyces cerevisiae 80-120 parts,
Lactobacillus plantarum 150-300 parts, Enteromorpha proteinase 200-300 parts.
2. according to claim 1, it is characterised in that the preparation method of microbial bacterial agent of the present invention is divided into three steps
Suddenly, affiliated step 1 is actication of culture：Each strain is inoculated with corresponding culture medium by the ratio；Its
In, the bacillus subtilis activation medium uses beef-protein medium, is formulated as beef extract 0.3g/L, peptone
1.0g/L, sodium chloride 0.5g/L, sodium hydroxide 0.5g/L, cultivation temperature are 35-40 DEG C；The saccharomyces cerevisiae activation medium is adopted
With brewer's wort potato sucrose culture medium, it is formulated as the g/L of dehydrated potato powder 7.0, sucrose 20.0 g/L, cultivation temperature 30-35
℃；The stearothermophilus spore bud baccilus medium uses glucase peptone water medium, is formulated as peptone
5.0g/L, glucose 0.25g/L, bromocresol purple 0.010g/L, cultivation temperature are 50-52 DEG C；The hot streptomyces diastaticus training
Support base and use yeast extract malt extract culture medium, be formulated as yeast extract 2.0g/L, malt extract 5.0 g/L, glucose 2.0g/L, culture
Temperature is 30-35 DEG C；The Lactobacillus plantarum activation medium uses lactic acid bacteria culture medium, is formulated as albumen 5.0g/L, ox
Meat extract 5.0g/L, yeast extract 2.5g/L, glucose 10.0g/L, Tween 80 0.5g/L, dipotassium hydrogen phosphate 1.0g/L, sodium acetate
2.5 g/L, the g/L of dibasic ammonium citrate 1, epsom salt 0.025g/L, cultivation temperature is 20-35 DEG C.
3. according to claim 2, it is characterised in that the preparation method step 2 of microbial bacterial agent of the present invention is mixing
Fermentation：By the good bacillus subtilis of activation culture, hot streptomyces diastaticus, stearothermophilus spore bud bacillus, saccharomyces cerevisiae, plant
Lactobacillus is mixed in the ratio, is inoculated into fermentation tank, and volume shared by fermentation medium is 60%, and inoculation volume is 3%, is used
Fermentation medium is beancake powder 5-20g/L, corn steep liquor 5-15g/L, urea 5-10g/L, magnesium sulfate 0.1-0.5g/L, calcium carbonate 1-
5g/L, sodium sulphate 0.1-0.5 g/L, glucose 15-20 g/L, 30-45 DEG C of cultivation temperature, after high density stirring fermentation, obtain
To mixed bacteria liquid.
4. according to claim 2, it is characterised in that the preparation method step 3 of microbial bacterial agent of the present invention is drying
Mixing：The mixed bacteria liquid of atomization is made by dried microorganism bacterium powder using the method for spray drying, by dried microorganism bacterium powder with
After enteromorpha protein enzyme crystal is stirred mixing again by proportion of composing, microbial bacterial agent of the present invention is formed.
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN108690819A (en) * | 2018-05-30 | 2018-10-23 | 叶荫人 | A kind of Geobacillus stearothermophilus bacterial strain and its apply and methods for using them |
| CN109504623A (en) * | 2018-11-20 | 2019-03-22 | 江南大学 | One plant of thermophilic short bud bacillus for producing amylase and its application |
| CN110408576A (en) * | 2019-08-26 | 2019-11-05 | 厦门市民安创享实业有限公司 | A kind of rubbish from cooking leavening and preparation method thereof |
| CN111286478A (en) * | 2020-03-09 | 2020-06-16 | 西南交通大学 | Grease degradation composite bacterial agent and preparation method and application thereof |
| CN111961606A (en) * | 2020-06-24 | 2020-11-20 | 人与自然环保生物科技(南京)有限公司 | Kitchen waste composite microbial degradation microbial inoculum and preparation method and application thereof |
| CN112007315A (en) * | 2020-08-28 | 2020-12-01 | 南京沃德思环保科技有限公司 | Microbial environment-friendly garbage treating agent |
| CN112094782A (en) * | 2020-09-27 | 2020-12-18 | 深圳市家家分类环保技术开发有限公司 | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof |
| CN112111425A (en) * | 2020-09-10 | 2020-12-22 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage and preparation method thereof |
| CN112481152A (en) * | 2020-11-05 | 2021-03-12 | 上海应用技术大学 | Microbial agent-enzyme preparation compound and method for degrading kitchen garbage by using same |
| CN112616997A (en) * | 2021-01-06 | 2021-04-09 | 安徽工业大学 | Method for preparing feed by utilizing kitchen waste |
| CN113881600A (en) * | 2021-10-29 | 2022-01-04 | 内蒙古自治区农牧业科学院 | Liquid culture medium for simultaneously culturing two different bacilli and culture method thereof |
| CN114904879A (en) * | 2022-03-02 | 2022-08-16 | 上海市园林科学规划研究院 | Preparation method of kitchen waste biogas residue with low phytotoxicity |
| CN115125168A (en) * | 2022-06-21 | 2022-09-30 | 华沃德源环境技术(济南)有限公司 | Composite bacterial enzyme agent for kitchen waste treatment and preparation method and application thereof |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531412A (en) * | 2018-03-20 | 2018-09-14 | 大连三净集团有限公司 | A kind of preparation method of ECMD Efficient Rings control microbial deodorant |
| CN108690819A (en) * | 2018-05-30 | 2018-10-23 | 叶荫人 | A kind of Geobacillus stearothermophilus bacterial strain and its apply and methods for using them |
| CN109504623A (en) * | 2018-11-20 | 2019-03-22 | 江南大学 | One plant of thermophilic short bud bacillus for producing amylase and its application |
| CN110408576A (en) * | 2019-08-26 | 2019-11-05 | 厦门市民安创享实业有限公司 | A kind of rubbish from cooking leavening and preparation method thereof |
| CN110408576B (en) * | 2019-08-26 | 2021-04-13 | 厦门市民安创享实业有限公司 | Kitchen waste leavening agent and preparation method thereof |
| CN111286478A (en) * | 2020-03-09 | 2020-06-16 | 西南交通大学 | Grease degradation composite bacterial agent and preparation method and application thereof |
| CN111961606A (en) * | 2020-06-24 | 2020-11-20 | 人与自然环保生物科技(南京)有限公司 | Kitchen waste composite microbial degradation microbial inoculum and preparation method and application thereof |
| CN112007315A (en) * | 2020-08-28 | 2020-12-01 | 南京沃德思环保科技有限公司 | Microbial environment-friendly garbage treating agent |
| CN112111425A (en) * | 2020-09-10 | 2020-12-22 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage and preparation method thereof |
| CN112094782A (en) * | 2020-09-27 | 2020-12-18 | 深圳市家家分类环保技术开发有限公司 | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof |
| CN112481152A (en) * | 2020-11-05 | 2021-03-12 | 上海应用技术大学 | Microbial agent-enzyme preparation compound and method for degrading kitchen garbage by using same |
| CN112616997A (en) * | 2021-01-06 | 2021-04-09 | 安徽工业大学 | Method for preparing feed by utilizing kitchen waste |
| CN113881600A (en) * | 2021-10-29 | 2022-01-04 | 内蒙古自治区农牧业科学院 | Liquid culture medium for simultaneously culturing two different bacilli and culture method thereof |
| CN114904879A (en) * | 2022-03-02 | 2022-08-16 | 上海市园林科学规划研究院 | Preparation method of kitchen waste biogas residue with low phytotoxicity |
| CN115125168A (en) * | 2022-06-21 | 2022-09-30 | 华沃德源环境技术(济南)有限公司 | Composite bacterial enzyme agent for kitchen waste treatment and preparation method and application thereof |
| CN115125168B (en) * | 2022-06-21 | 2023-09-26 | 华沃德源环境技术(济南)有限公司 | Composite bacterial enzyme agent for kitchen waste treatment and preparation method and application thereof |
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