CN104212746B - A kind of changing food waste of salt tolerant processes composite bacteria agent and its preparation method and application - Google Patents
A kind of changing food waste of salt tolerant processes composite bacteria agent and its preparation method and application Download PDFInfo
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- CN104212746B CN104212746B CN201410468958.3A CN201410468958A CN104212746B CN 104212746 B CN104212746 B CN 104212746B CN 201410468958 A CN201410468958 A CN 201410468958A CN 104212746 B CN104212746 B CN 104212746B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention discloses a kind of changing food waste of salt tolerant processes composite bacteria agent, the composite bacteria agent is made up of composite thallus and carrier auxiliary material, the composite thallus include based process thalline, the based process thalline is made up of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae, wherein, in based process thalline, bacillus subtilis accounts for the 30～65% of total viable count, and bacillus coagulans accounts for the 20～65% of total viable count, and saccharomyces cerevisiae accounts for the 5～50% of total viable count.The composite bacteria agent of the present invention is applied to the kitchen garbage treater with insulation, ventilation and agitating function, microorganism contained by microbial inoculum can form stable Tiny ecosystem, and it is resistant to high salinity, therefore process the residue after changing food waste to continue to process changing food waste next time as microbial inoculum, realize once throwing bacterium Long-Time Service.
Description
Technical field
The present invention relates to technical field of waste treatment, more particularly to the method and microbial inoculum of changing food waste process.
Background technology
With China's expanding economy and the quickening of urbanization process, the discharge capacity of domestic waste is increasing, its
Ratio shared by middle changing food waste day by day rises, and has accounted for the 37～62% of domestic waste.Due to containing in changing food waste
Substantial amounts of carbohydrate, starch, protein, and salt and fat content are also very high, if be not acted upon, changing food waste easily becomes
Matter, corruption, harmful microorganism and mosquitos and flies are grown, produce a large amount of toxin and distribute foul gas, appearance of city can be had a strong impact on, damaged
Evil resident is healthy, welding quality.But changing food waste has abundant nutrient, if energy effective process can become tool
There is the resource of certain value.
Existing biological treatment method for kitchen garbage has aerobic fermentation compost, anaerobic fermentation to make compost, anaerobic fermentation production is raised
Material, producing methane through anaerobic fermentation etc..Anaerobic fermentation often produces larger stink, and fermentation time is up to 1～3 month, leads to
Factory's operation is only applicable to often.The aerobic fermentation compost time, the stink for producing that ferments was relatively small within 1 month.Aerobic
Ferment composting technology can depart from factory in theory, break the whole up into parts and in street, cell, restaurant, family realize changing food waste in source
Head treatment in situ.To realize changing food waste in source treatment in situ using aerobic composting technology, it is necessary to while meeting efficient process
Daily changing food waste, once throwing bacterium Long-Time Service, peculiar smell are little, safe and harmless these conditions of bacterial classification, otherwise it is difficult to promote.Great majority
Compost bacterium can not all meet, and need constantly to put into the microbial inoculum of activation, otherwise degraded effect
Fruit is deteriorated and peculiar smell is very big.Therefore, existing aerobic compost microbial inoculum is difficult the requirement for meeting family and restaurant degraded changing food waste,
Minimizing and the resource of food wastes can not be realized from source.
In view of mould serves Main Function in traditional Natural compost, the aerobic compost of domestic invention processes meal at present
The microbial inoculum of kitchen rubbish is generally using mould as major function bacterium.The patent of such as Application No. CN CN201010272823.1 is situated between
The microbial inoculum for continuing uses Mucor, the microbial inoculum that the patent of Application No. CN201210067350.0 is introduced using Mucor, aspergillus, trichoderma,
Mould.It is although less without the microbial inoculum of mould but also have, such as the patent of Application No. CN201110353751.8, the bacterium of introduction
Agent is made up of lactobacillus acidophilus and saccharomycete.Although mould can improve the rotten degree of composting production, have the disadvantage fermenting speed
It is relatively slow and be not suitable for once throwing bacterium Long-Time Service.The extensive heap of composting plant is commonly used to the microbial inoculum that mould is main function bacterium
Fertilizer, a batch at least heap two weeks, per batch new microbial inoculum of addition.Because mould is irreproducible under agitation passing on, and it is high
Effect aerobic compost must come mixed material and supply oxygen by stirring again, so der Pilz microbial inoculum is in Small-scale kitchen garbage process
The application of aspect is restricted.If by mould for Small-scale kitchen garbage process, as microbial inoculum Central Plains is containing a large amount of mould
The digestive ferment of bacterium secretion, initial stage treatment effeciency is very high, but constantly can decline as enzyme is constantly diluted treatment effeciency.
With the compost bacterium that yeast or bacterium are main function bacterium, growth and breeding speed is fast, suitable to stir, but also may not necessarily
Bacterium Long-Time Service is thrown once.There is a following difficult problem to need to overcome：(1) compost bacterium will can form stable Tiny ecosystem, or extremely
Can be with common fermentation between few bacterial classification.(2) there may be much harmful spoilage organisms in changing food waste itself and environment, compost bacterium will
There is the ability for suppressing harmful corruption bacteria growing.(3) salt content of changing food waste about 1～1.5%, these salt branches are in changing food waste
Accumulation in processor, makes the salt content of composting reach 3～5%, therefore compost bacterium must be resistant to high salt point.Must simultaneously gram
Take a three above difficult problem to realize once throwing bacterium Long-Time Service, but domestic existing compost bacterium is generally unable to reach and wants
Ask, so service life is often less than 3 months.
The content of the invention
Present invention aim to address the defect of above-mentioned prior art, there is provided a kind of high degradation efficiency, free from extraneous odour, salt tolerant,
And can once throw the changing food waste process microbial inoculum of bacterium Long-Time Service.
To achieve these goals, this invention takes technical scheme below：A kind of changing food waste of salt tolerant processes compound
Microbial inoculum, the composite bacteria agent are made up of composite thallus and carrier auxiliary material, and the composite thallus include based process thalline, the base
Plinth processes thalline and is made up of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae, wherein, withered grass in based process thalline
Bacillus accounts for the 30～65% of total viable count, and bacillus coagulans accounts for the 20～65% of total viable count, and saccharomyces cerevisiae accounts for total work
The 5～50% of bacterium number.
Preferably, in the based process thalline, bacillus subtilis accounts for the 40～55% of total viable count, condenses gemma bar
Bacterium accounts for the 30～45% of total viable count, and saccharomyces cerevisiae accounts for the 15～30% of total viable count.
Preferably, viable bacteria content of the bacillus subtilis in composite bacteria agent described in the based process thalline for (2～
9)×108The viable bacteria content of CFU/g, bacillus coagulans in composite bacteria agent is (1～8) × 108CFU/g, saccharomyces cerevisiae are multiple
It is (3～9) × 10 to close the viable bacteria content in microbial inoculum7CFU/g。
Further, the composite thallus can be with addition function bacterial classification, the function bacterium outside based process thalline
Planting must be with based process thalline symbiosis.The function bacterial classification includes efficient degreasing bacterium, or efficiently plastics bacterium, or both it is mixed
Close.
Specifically, the carrier auxiliary material needs the composition and loose ventilative load of carbon source and nitrogen source by providing Institute of Micro-biology
Body is mixed.
Preferably, the carrier auxiliary material is mixed by bean cake powder, corn flour, Icing Sugar, wood wool.
It is further preferred that the weight proportion in the carrier auxiliary material is bean cake powder：Corn flour：Icing Sugar：Wood wool=(2
～5)：(2～3)：(1～3)：(0.5～1).
Used as another optional embodiment, the carrier auxiliary material rice bran and peanut press pulp are preferably used as carbon source and nitrogen source
Wood wool, or bagasse powder, or peanut shell powder, or two or more mixing is used as loose ventilative carrier.
A kind of changing food waste of salt tolerant as above processes the preparation method of composite bacteria agent, and which comprises the steps：
(1) to composite bacteria agent in various bacterium carry out independent Spawn incubation respectively, then by the bacterium that will be obtained is collected by centrifugation
Body mixes, and makes composite thallus.
(2) carrier auxiliary material each component is mixed and made into into carrier auxiliary material.
(3) take the carrier auxiliary material obtained by wet-milling shape composite thallus and step 2 obtained by step 1 and be mixed to prepare composite bacteria agent,
The wet-milling shape composite thallus are (0.8～1.3) with the weight ratio of carrier auxiliary material：1, preferably 1：1, by composite bacteria agent dry to
Moisture is 15%, then vacuumizes packaging again.
Preferably, it is by half carrier auxiliary material in the composite thallus of wet-milling shape in step 1 first and step 2 in the step 3
Mixing, is dried after being 15% to moisture, then is mixed with second half carrier auxiliary material.
As above application of arbitrary composite bacteria agent in changing food waste process.
The salt tolerant changing food waste of the present invention processes microbial inoculum, and which utilizes 3 kinds of beneficial bacteriums harmless to environment and human body, by proportioning
Combination forms stable Tiny ecosystem.Beneficial bacterium in formula is the relation of mutual reciprocity and mutual benefit, and common fermentation can efficiently by kitchen rubbish
Rubbish is converted into humus.Jing repetition tests, find the ability that the microbial inoculum has efficient degradation changing food waste, free from extraneous odour, resistance to high salt
Point, and can once throw bacterium Long-Time Service.The humus of conversion can adsorb the salinity in changing food waste, so as to reduce soluble-salt
The content divided, so can both make beneficial compost bacterium keep higher activity, can mitigate due to using changing food waste heap again
Fertilizer applies fertilizer and the caused soil salinization.Beneficial bacterium in formula can suppress while efficient degradation changing food waste again
The generation of stink.
The composite bacteria agent of the present invention is applied to the kitchen garbage treater with insulation, ventilation and agitating function, most suitable work
Make temperature for 36～45 DEG C.Microorganism contained by microbial inoculum can form stable Tiny ecosystem, and be resistant to high salinity, therefore process
Residue after changing food waste can continue to process changing food waste as microbial inoculum next time, realize once throwing bacterium Long-Time Service.Due to multiple
In closing microbial inoculum, types of spawn is few, and culture presevation, rejuvenation and fermentation costs workload are greatly reduced, and the workload accounts for microbial inoculum life
More than the 80% of totle drilling cost is produced, therefore greatly reduces the production cost of microbial inoculum.
Specific embodiment
In the present invention, formation base processes three bacterial classifications of thalline：Bacillus subtilis, bacillus coagulans and wine brewing ferment
It is female to obtain from the purchase of various market channels, for the bacterial strain for being used and have no special requirements.And in inventive embodiments
Bacterial strain comes from Guangdong Province's Culture Collection：Bacillus subtilis numbering GIM 1.222, bacillus coagulans numbering
GIM 1.421, saccharomyces cerevisiae numbering GIM 2.135.
Embodiment one
Preparation example of this example for composite bacteria agent.
(1) composite thallus are prepared
1) inclined-plane culture：
Bacillus subtilis is seeded in into nutrient agar slant medium respectively, bacillus coagulans is seeded in MRS inclined-planes
Culture medium, saccharomyces cerevisiae are seeded in PDA slant mediums.Bacillus subtilis and bacillus coagulans are cultivated at a temperature of 37 DEG C
48h, saccharomyces cerevisiae cultivate 72h at a temperature of 28 DEG C, carry out the activation of bacterial strain.
2) primary seed solution culture：
Bacillus subtilis after activation obtained by step 1, bacillus coagulans, saccharomyces cerevisiae are inoculated in respectively
250mL fluid nutrient mediums.Bacillus subtilis and bacillus coagulans cultivate 48h at a temperature of 37 DEG C, and saccharomyces cerevisiae is at 28 DEG C
At a temperature of cultivate 48h, carry out the activation of bacterial strain.
3) fermentation tank culture：
Primary seed solution obtained by step 2 is inoculated in 10L fermentor liquid culture mediums respectively and is cultivated.Bacillus subtilis
Bacterium, bacillus coagulans cultivate 48h at a temperature of 37 DEG C, and saccharomyces cerevisiae cultivates 48h at a temperature of 28 DEG C.
The Liquid Culture based formulas of bacillus subtilis：Glucose 1%, peptone 1%, beef extract 0.5%, sodium chloride
0.5%, pH7～7.4.
The Liquid Culture based formulas of bacillus coagulans：Emulsification peptone 1.5%, yeast extract 0.5%, potassium dihydrogen phosphate
0.2%th, glucose 1%, tomato juice 10%, Tween 80 0.1%, pH6.6～7.
The Liquid Culture based formulas of saccharomyces cerevisiae：Malt dry powder adds four times of water, and the water-bath at 65 DEG C is saccharified 3～4 hours, uses
Iodine solution is determined to without blue reaction；Boil, filter, be diluted to 5～6 Baume degrees (Be °) or 10 ° of pols, pH6.0～6.4.
4) zymotic fluid obtained by step 3 is collected by centrifugation respectively and obtains each bacteroid, by 1：1：1 mass parts are mixed
Wet-milling shape composite thallus.
(2) prepare carrier auxiliary material
Bean cake powder, corn flour, Icing Sugar, wood wool are pressed into 3：3：2：0.5 mass parts are mixed and made into carrier auxiliary material.
(3) prepare composite bacteria agent
Wet-milling shape composite thallus are mixed with the carrier auxiliary material of identical weight, it is 15% to dry to moisture, is dried bacterium
Body accounts for the 30% of microbial inoculum weight.The work of the bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae in composite bacteria agent
Bacterial content is to be followed successively by (4～6) × 108CFU/g, (3～5) × 108CFU/g, (5～7) × 107CFU/g。
Embodiment two
Preparation example of this example for microbial inoculum.
(1) composite thallus are prepared
Each bacterial classification is individually cultivated by the training method of embodiment one, be then centrifuged for collects thalline, and press following mass parts
It is mixed and made into wet-milling shape composite thallus, bacillus subtilis：Bacillus coagulans：Saccharomyces cerevisiae=2：1：1.
(2) prepare carrier auxiliary material
Bean cake powder, corn flour, Icing Sugar, wood wool are pressed into 4：2：2：0.8 mass parts are mixed and made into carrier auxiliary material, and carrier is auxiliary
The gross weight of material is identical with wet-milling shape composite thallus.
(3) prepare composite bacteria agent
Wet-milling shape composite thallus are mixed with half carrier auxiliary material, dry to moisture be 15% after, then with second half
Carrier auxiliary material mixes.So, thalline can obtain drying effect evenly, be conducive to keeping activity.Microbial inoculum water content about 15%,
Be dried that thalline accounts for microbial inoculum weight 30%.The bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae are in composite bacteria agent
In viable bacteria content to be followed successively by (7～9) × 108CFU/g, (2～4) × 108CFU/g, (4～6) × 107CFU/g。
Embodiment three
Preparation example of this example for microbial inoculum.
(1) composite thallus are prepared
Each bacterial classification is individually cultivated by the training method of embodiment one, be then centrifuged for collects thalline, and press following mass parts
Wet-milling shape based process thalline is mixed and made into, wherein：Bacillus subtilis：Bacillus coagulans：Saccharomyces cerevisiae=2：1.2：
0.8。
Further, add the efficient plastic degradation bacterium of function thalline in based process thalline and prepare composite thallus, by matter
Amount part meter, processes the 20% of thalline based on function thalline addition.
(2) prepare carrier auxiliary material
Rice bran, peanut press pulp, Icing Sugar, bagasse powder are pressed into 4：1：1：1 mass parts are mixed and made into carrier auxiliary material, carrier auxiliary material
Gross weight it is identical with wet-milling shape composite thallus.
(3) prepare composite bacteria agent
Wet-milling shape composite thallus are mixed with half carrier auxiliary material, dry to moisture be 15% after, then with second half
Carrier auxiliary material mixes.Microbial inoculum water content about 15%, be dried that thalline accounts for microbial inoculum weight 30%.It is the bacillus subtilis, solidifying
The viable bacteria content of knot bacillus and saccharomyces cerevisiae in composite bacteria agent is to be followed successively by (7～9) × 108CFU/g, (3～5) ×
108CFU/g, (3～5) × 107CFU/g。
Example IV
This example is with Degrading experiment of the microbial inoculum to changing food waste obtained in embodiment one.
(1) composition of changing food waste：The food collected in the swill bucket of dining room, removes bulk, the hard parts such as bone.
(2) composite bacteria agent 2.5kg obtained in embodiment one is put in small-sized kitchen garbage treater, add water 1L and
Brown sugar 350g activates 24h.Add changing food waste 1kg after activation daily, it is continuous to add 30 days.
(3) measure of changing food waste degradation rate：When weighing does not add changing food waste, the gross weight of processor and microbial inoculum is
20.5kg, adds 1kg changing food wastes to add 30kg altogether for continuous 30 days, then weighs the gross weight of processor and composting for 28.2kg.
According to the formula of loss of weight efficiency
A=20.5+30=50.5 (kg), B=28.2 (kg), C=30 (kg),
The loss of weight efficiency for calculating changing food waste after processing 30 days is 74.3%.
Degrading experiment is carried out with microbial inoculum obtained in embodiment two, three to changing food waste, step is ibid.Table 1 below lists three
The result of the test of Formulation Example：
Table 1
| One microbial inoculum of embodiment | Two microbial inoculum of embodiment | Three microbial inoculum of embodiment | |
| Loss of weight efficiency | 74.3% | 78.5% | 74.1% |
Embodiment five
This example is that the test of soluble-salt content in kitchen waste compost material is reduced with microbial inoculum obtained in embodiment one.
(1) composition of changing food waste：The food collected in the swill bucket of dining room, removes bulk, the hard parts such as bone.
(2) microbial inoculum 2.5kg obtained in embodiment one is put in small-sized kitchen garbage treater, adds water 1L and brown sugar
350g activates 24h.Add the changing food waste 1kg that mean salt amount is 1.2% after activation daily.Changing food waste heap after testing 30 days
Fertilizer net weight 9.3kg, soluble-salt content are 1.8%.According to the formula of less salt efficiency
X=30 × 0.012 ÷ 9.3 × 100%=3.9%, Y=1.8%,
The less salt efficiency for calculating kitchen waste compost material after processing 30 days is 53.8%.
Wherein, soluble-salt content is measured with electrical conductivity method：(1) 5g samples shake mixing 30min in 50mL distilled water,
Filtering solution；(2) survey sample solution electrical conductivity and normal concentration (0%, 0.05%, 0.1%, 0.15%, 0.3%,
0.5%) electrical conductivity of sodium chloride solution；(3) draw " electrical conductivity-sodium chloride mass fraction " calibration curve；(4) calculate with
The salt content of the mass fraction of the sodium chloride solution of the conductivity values such as sample solution, as sample solution；(5) as sample is molten
Liquid is diluted with water 10 times by sample and obtains, so the soluble-salt content of sample is equal to 10 times of sample solution salt content.
Carry out reducing the test of soluble-salt content in kitchen waste compost material with microbial inoculum obtained in embodiment two, three, step
Suddenly ibid.Table 2 below lists the result of the test of three Formulation Examples：
Table 2
| One microbial inoculum of embodiment | Two microbial inoculum of embodiment | Three microbial inoculum of embodiment | |
| Less salt efficiency | 53.8% | 52.6% | 52.1% |
Claims (8)
1. a kind of changing food waste of salt tolerant processes composite bacteria agent, it is characterised in that：The composite bacteria agent is by composite thallus and carrier
Auxiliary material is constituted, and the composite thallus are separately formed by based process thalline or by based process thalline and common group of function bacterial classification
Into, the based process thalline is made up of bacillus subtilis, bacillus coagulans and saccharomyces cerevisiae, wherein, based process
In thalline, bacillus subtilis accounts for the 30～65% of total viable count, and bacillus coagulans accounts for the 20～65% of total viable count, wine brewing
Yeast accounts for the 5～50% of total viable count；The carrier auxiliary material is by providing composition that Institute of Micro-biology needs carbon source and nitrogen source and loose
The carrier of gas is mixed.
2. the changing food waste of salt tolerant according to claim 1 processes composite bacteria agent, it is characterised in that：The based process bacterium
In body, bacillus subtilis accounts for the 40～55% of total viable count, and bacillus coagulans accounts for the 30～45% of total viable count, ferment of making wine
Mother accounts for the 15～30% of total viable count.
3. the changing food waste of salt tolerant according to claim 1 processes composite bacteria agent, it is characterised in that：The based process bacterium
Viable bacteria content of the bacillus subtilis described in body in composite bacteria agent is (2～9) × 108CFU/g, bacillus coagulans are multiple
It is (1～8) × 10 to close the viable bacteria content in microbial inoculum8The viable bacteria content of CFU/g, saccharomyces cerevisiae in composite bacteria agent be (3～9) ×
107CFU/g。
4. the changing food waste of salt tolerant according to claim 1 processes composite bacteria agent, it is characterised in that：The carrier auxiliary material by
Bean cake powder, corn flour, Icing Sugar, wood wool are mixed, and its weight proportion is bean cake powder：Corn flour：Icing Sugar：Wood wool=(2
～5)：(2～3)：(1～3)：(0.5～1).
5. the changing food waste of salt tolerant according to claim 1 processes composite bacteria agent, it is characterised in that：The carrier auxiliary material is used
Rice bran and peanut press pulp are used as carbon source and nitrogen source.
6. a kind of changing food waste of salt tolerant as described in Claims 1 to 5 any claim processes the preparation side of composite bacteria agent
Method, it is characterised in that comprise the steps：
(1) to composite bacteria agent in various bacterium carry out independent Spawn incubation respectively, then mixed by the thalline being collected by centrifugation acquisition
Close, make wet-milling shape composite thallus；
(2) carrier auxiliary material each component is mixed and made into into carrier auxiliary material；
(3) take the carrier auxiliary material obtained by wet-milling shape composite thallus and step 2 obtained by step 1 and be mixed to prepare composite bacteria agent, it is described
Wet-milling shape composite thallus are (0.8～1.3) with the weight ratio of carrier auxiliary material：1, composite bacteria agent is dried to moisture it is
15%, packaging is then vacuumized again.
7. the changing food waste of salt tolerant according to claim 6 processes the preparation method of composite bacteria agent, it is characterised in that：It is described
It is that the composite thallus of wet-milling shape in step 1 are first mixed with half carrier auxiliary material in step 2 in step 3, dries to moisture
After 15%, then mix with second half carrier auxiliary material.
8. application of the arbitrary composite bacteria agent of Claims 1 to 5 in changing food waste process.
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| CN104774788B (en) * | 2015-03-30 | 2017-12-29 | 天津师范大学 | Lawn salt tolerant strengthens the preparation method and application of complex microbial community in garbage compost |
| CN105567616B (en) * | 2016-03-22 | 2020-03-24 | 青岛东海药业有限公司 | Microbial composition and application thereof |
| CN106977250B (en) * | 2017-03-28 | 2018-06-22 | 惠州市牧兴农业科技有限公司 | A kind of microbial premix and organic compost |
| CN106929449B (en) * | 2017-03-30 | 2019-10-11 | 汉宇集团股份有限公司 | It is a kind of degrade grease bacillus subtilis and its application |
| CN107043725A (en) * | 2017-04-25 | 2017-08-15 | 西安鑫汉宝生物科技有限公司 | Method of bacillus subtilis and bacillus coagulans mixed fermentation and application thereof |
| CN112094782A (en) * | 2020-09-27 | 2020-12-18 | 深圳市家家分类环保技术开发有限公司 | Composite microbial inoculum for degrading kitchen garbage and preparation method thereof |
| CN112159783A (en) * | 2020-10-28 | 2021-01-01 | 深圳市家家分类环保技术开发有限公司 | Acid-resistant salt-resistant microbial agent for degrading kitchen garbage and preparation method thereof |
| CN112400396A (en) * | 2020-11-09 | 2021-02-26 | 华东师范大学 | Method for improving soil of reclaimed land in reclamation area |
| CN112980825B (en) * | 2020-12-23 | 2023-04-07 | 南方医科大学 | Microbial agent for eliminating kitchen waste and preparation method and application thereof |
| CN113481118B (en) * | 2021-06-04 | 2022-08-19 | 上海交通大学 | Acid-resistant, salt-resistant, heat-resistant and organic matter degradation bacillus subtilis and application thereof in kitchen waste recycling |
| CN114231428B (en) * | 2021-07-15 | 2023-09-01 | 中农新科(苏州)有机循环研究院有限公司 | Low-temperature acid-resistant saccharomyces cerevisiae as well as screening method and application thereof |
| CN113956998A (en) * | 2021-07-31 | 2022-01-21 | 菏泽嘉麟生物科技有限公司 | Method for preparing thermophilic deodorizing city domestic garbage treating microbial inoculum and application thereof |
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| CN103865847B (en) * | 2014-03-06 | 2016-06-08 | 深圳市三林生物科技工程有限公司 | Compound microbial preparation for garbage deodorization and preparation method thereof |
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