CN112111425A - Environment-friendly biological agent for treating organic garbage and preparation method thereof - Google Patents
Environment-friendly biological agent for treating organic garbage and preparation method thereof Download PDFInfo
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- 239000003124 biologic agent Substances 0.000 title claims abstract description 27
- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 23
- 238000002360 preparation method Methods 0.000 title abstract description 9
- 239000000843 powder Substances 0.000 claims abstract description 137
- 238000000855 fermentation Methods 0.000 claims abstract description 29
- 241000186660 Lactobacillus Species 0.000 claims abstract description 16
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 16
- 241000589152 Azotobacter chroococcum Species 0.000 claims abstract description 11
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 11
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 11
- 241000194107 Bacillus megaterium Species 0.000 claims abstract description 11
- 241000194103 Bacillus pumilus Species 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 11
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 11
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims abstract description 11
- 108010059892 Cellulase Proteins 0.000 claims abstract description 11
- 241000186673 Lactobacillus delbrueckii Species 0.000 claims abstract description 11
- 241000108664 Nitrobacteria Species 0.000 claims abstract description 11
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 11
- 241000589516 Pseudomonas Species 0.000 claims abstract description 11
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims abstract description 11
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 11
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims abstract description 11
- 229940106157 cellulase Drugs 0.000 claims abstract description 11
- 108010062085 ligninase Proteins 0.000 claims abstract description 11
- 244000068988 Glycine max Species 0.000 claims abstract description 10
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 8
- 241000589180 Rhizobium Species 0.000 claims abstract description 7
- DBPRUZCKPFOVDV-UHFFFAOYSA-N Clorprenaline hydrochloride Chemical compound O.Cl.CC(C)NCC(O)C1=CC=CC=C1Cl DBPRUZCKPFOVDV-UHFFFAOYSA-N 0.000 claims abstract description 6
- 230000004151 fermentation Effects 0.000 claims description 27
- 238000009655 industrial fermentation Methods 0.000 claims description 12
- 239000002068 microbial inoculum Substances 0.000 claims description 12
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 10
- 240000008042 Zea mays Species 0.000 claims description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 10
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 10
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 claims description 10
- 235000005822 corn Nutrition 0.000 claims description 10
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 claims description 5
- 241000254109 Tenebrio molitor Species 0.000 claims description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 5
- 239000004202 carbamide Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-M dihydrogenphosphate Chemical compound OP(O)([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-M 0.000 claims description 5
- 238000001035 drying Methods 0.000 claims description 5
- 238000004108 freeze drying Methods 0.000 claims description 5
- 235000012054 meals Nutrition 0.000 claims description 5
- 239000012452 mother liquor Substances 0.000 claims description 5
- 239000010815 organic waste Substances 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000004576 sand Substances 0.000 claims description 5
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims 1
- 239000002910 solid waste Substances 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 2
- 238000006555 catalytic reaction Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 210000003608 fece Anatomy 0.000 abstract 1
- 239000010871 livestock manure Substances 0.000 abstract 1
- 239000002689 soil Substances 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 230000000593 degrading effect Effects 0.000 description 4
- 239000003344 environmental pollutant Substances 0.000 description 4
- 231100000719 pollutant Toxicity 0.000 description 4
- 239000010802 sludge Substances 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 3
- 238000006731 degradation reaction Methods 0.000 description 3
- 229920001542 oligosaccharide Polymers 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000006065 biodegradation reaction Methods 0.000 description 2
- 244000144972 livestock Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000002957 persistent organic pollutant Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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Abstract
The invention relates to an environment-friendly biological agent for treating organic garbage and a preparation method thereof, wherein the environment-friendly biological agent comprises, by weight, 8-13 parts of bacillus licheniformis powder, 10-15 parts of bacillus pumilus powder, 13-16 parts of nitrobacteria powder, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of soybean rhizobium powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of pseudomonas fusca powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino-oligosaccharin, 0.05-1 part of brassinolide; the method can be applied to the treatment of different organic solid wastes, has low requirements on working conditions, can start self-fermentation at normal temperature, does not need high-temperature high-pressure catalysis, greatly shortens the treatment period to 24 hours to a week, is environment-friendly and harmless, and finally treats the product as biological bacterial manure to change waste into valuable.
Description
Technical Field
The invention relates to the field of microbial agents, in particular to an environment-friendly biological agent for treating organic garbage and a preparation method thereof.
Background
Most of the existing microbial inoculums can only treat sewage, the treatment of organic solid wastes needs a very harsh working environment (high temperature and high pressure), the treatment period is long (generally more than one month is needed), and the components of a treatment object are single (for example, only organic sludge can be treated singly), so that the requirement of large-scale treatment of organic solid wastes cannot be met. Finally, the existing microbial inoculum can not produce a final product with recycling value, and can not recycle organic waste resources.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides an environment-friendly biological agent for treating organic garbage and a preparation method thereof, which not only can independently and efficiently degrade various organic garbage such as organic sludge, kitchen garbage, landscaping garbage, livestock garbage and the like, but also can be directly used for the mixed treatment of the various organic garbage, has low requirement on working conditions, can start self-fermentation at normal temperature, does not need high-temperature high-pressure catalysis, greatly shortens the treatment period to 24 hours to a week, is environment-friendly and harmless, and can improve soil and change waste into valuable as a biological bacterial fertilizer which is a final treatment product.
In order to achieve the purpose, the invention adopts the technical scheme that: an environment-friendly biological agent for treating organic garbage comprises the following components in parts by weight, comprises 8-13 parts of bacillus licheniformis powder, 10-15 parts of bacillus pumilus powder, 13-16 parts of nitrobacteria powder, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of rhizobium japonicum powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of pseudomonas fusca powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino oligosaccharin and 0.05-1 part of brassinolide.
The invention also provides a preparation method of the environment-friendly biological agent for treating the organic garbage, which comprises the following steps:
s1: generating flora: adding bacillus licheniformis powder, bacillus pumilus powder, nitrobacteria powder, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, additionally adding 10-15 parts of peptone, 10-20 parts of lactobacillus fermentation mother liquor, 1-2 parts of brown sugar and 1-2 parts of urea, and performing constant-temperature fermentation for 10-14 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 10-20 parts of wort, 1-3 parts of monohydrogenphosphate and 1-2 parts of dihydrogen phosphate into the flora, adjusting the ph to 7-7.5, adding 0.3-1 part of calcium carbonate and 0.5-1 part of calcium nitrate to further activate the microbial inoculum, and continuously culturing on a shaking table for 10-24 hours;
s3, industrial fermentation propagation: on the basis of S2, adding 5-10 parts of corn germ meal, 10-12 parts of tenebrio molitor sand and 5-8 parts of corn bran into the microbial inoculum, performing industrial fermentation propagation for 1-3 days, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent.
Preferably, in S1, the temperature for constant temperature fermentation is 37 ℃.
Preferably, in S2, the speed of the shaker is 100-150 rpm.
Preferably, in S3, the temperature for industrial fermentation propagation is 40 ℃, and ph is 6.8-7.5.
Due to the application of the technical scheme, compared with the prior art, the invention has the following advantages:
1. the invention can be applied to the treatment of different organic solid wastes, the efficiency of degrading domestic sludge is about one week and is far stronger than other biodegradation methods (one to two months) in the market, the efficiency of degrading kitchen garbage is within 24 hours, the world leading level is achieved, the degradation of animal husbandry pollutants can be basically completed within 8 hours, the reaction condition is mild, a high-temperature high-pressure container is not needed, the fermentation can be started at normal temperature and normal pressure to degrade the organic pollutants, the reaction site is simple, the invention can be applied to rural cities, and only a basic stirring and heat-preserving device is needed;
2. all strains adopted by the invention do not contain pathogenic bacteria, have the effect of regulating and repairing soil, are fermented into nutrient soil after pollutant degradation is finished, and can be returned to the field, so that the garbage recycling treatment is really realized.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
The first embodiment is as follows:
the environment-friendly biological agent for treating organic garbage comprises, by weight, 13 parts of bacillus licheniformis powder, 15 parts of bacillus pumilus powder, 16 parts of nitrobacteria powder, 10 parts of lactobacillus powder, 10 parts of bacillus megaterium powder, 12 parts of bacillus subtilis powder, 9 parts of bacillus amyloliquefaciens powder, 5 parts of saccharomyces cerevisiae powder, 1 part of bacillus mucilaginosus powder, 1 part of soybean rhizobia powder, 2 parts of azotobacter chroococcum powder, 3 parts of pseudomonas fusca powder, 1 part of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.01 part of 90% amino-oligosaccharide and 0.09 part of brassinolide.
The invention also provides a preparation method of the environment-friendly biological agent for treating the organic garbage, which comprises the following steps:
s1: generating flora: adding bacillus licheniformis powder, bacillus pumilus powder, nitrobacteria powder, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, taking 100kg of fermentation as an example, additionally adding 10kg of peptone, 10kg of lactobacillus fermentation mother liquor, 1kg of brown sugar and 1kg of urea, and carrying out constant-temperature fermentation at 37 ℃ for 10 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 10kg of wort, 1kg of monohydrogenphosphate and 1kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 0.3kg of calcium carbonate and 0.5kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 24h on a shaking table at the speed of 100 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 5kg of corn germ meal, 10kg of tenebrio molitor sand and 5kg of corn bran into the microbial inoculum, carrying out 1-day industrial fermentation propagation at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
Example two:
the environment-friendly biological agent for treating organic garbage comprises, by weight, 10 parts of bacillus licheniformis powder, 13 parts of bacillus pumilus powder, 13 parts of nitrobacteria powder, 9 parts of lactobacillus powder, 9 parts of bacillus megaterium powder, 10 parts of bacillus subtilis powder, 12 parts of bacillus amyloliquefaciens powder, 6 parts of saccharomyces cerevisiae powder, 3 parts of bacillus mucilaginosus powder, 3 parts of soybean rhizobia powder, 3 parts of azotobacter chroococcum powder, 5 parts of pseudomonas fusca powder, 2 parts of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.03 part of 90% amino-oligosaccharide and 0.07 part of brassinolide.
The invention also provides a preparation method of the environment-friendly biological agent for treating the organic garbage, which comprises the following steps:
s1: generating flora: according to the weight parts, adding bacillus licheniformis powder, bacillus pumilus powder, nitrobacteria powder, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii, taking 100kg of fermentation as an example, additionally adding 12kg of peptone, 15kg of lactobacillus fermentation mother liquor, 1.5kg of brown sugar and 1.5kg of urea, and carrying out 37-degree constant-temperature fermentation for 12 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 15kg of wort, 2kg of monohydrogenphosphate and 1.5kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 0.5kg of calcium carbonate and 0.8kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 18h on a shaker at the speed of 120 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 8kg of corn germ meal, 11kg of tenebrio molitor sand and 6kg of corn bran into the microbial inoculum, carrying out industrial fermentation propagation for 2 days at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
Example three:
the environment-friendly biological agent for treating organic garbage comprises, by weight, 8 parts of bacillus licheniformis powder, 10 parts of bacillus pumilus powder, 13 parts of nitrobacteria powder, 8 parts of lactobacillus powder, 7 parts of bacillus megaterium powder, 10 parts of bacillus subtilis powder, 12 parts of bacillus amyloliquefaciens powder, 8 parts of saccharomyces cerevisiae powder, 5 parts of bacillus mucilaginosus powder, 5 parts of soybean rhizobia powder, 4 parts of azotobacter chroococcum powder, 5 parts of pseudomonas fusca powder, 3 parts of lactobacillus delbrueckii, 1 part of cellulase, 0.9 part of ligninase, 0.01 part of 90% amino-oligosaccharide and 0.09 part of brassinolide.
The invention also provides a preparation method of the environment-friendly biological agent for treating the organic garbage, which comprises the following steps:
s1: generating flora: adding bacillus licheniformis powder, bacillus pumilus powder, nitrobacteria powder, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, taking 100kg of fermentation as an example, additionally adding 15kg of peptone, 20kg of lactobacillus fermentation mother liquor, 2kg of brown sugar and 2kg of urea, and carrying out constant-temperature fermentation at 37 ℃ for 14 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 20kg of wort, 3kg of monohydrogenphosphate and 2kg of dihydrogenphosphate into the flora, adjusting the ph to 7-7.5, adding 1kg of calcium carbonate and 1kg of calcium nitrate to further activate the microbial inoculum, and continuously culturing for 10 hours on a shaking table at the speed of 150 rpm;
s3, industrial fermentation propagation: on the basis of S2, adding 10kg of corn germ meal, 12kg of tenebrio molitor sand and 8kg of corn bran into the microbial inoculum, carrying out industrial fermentation propagation for 3 days at 40 ℃ and ph of 6.8-7.5, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent, wherein the effective period is two years.
The environment-friendly biological agent obtained by the three embodiments can be applied to treatment of different organic solid wastes, the efficiency of degrading domestic sludge is about one week and is far stronger than that of other biodegradation methods (one to two months) in the market, the efficiency of degrading kitchen garbage is within 24 hours, the world leading level is achieved, the degradation of livestock pollutants can be basically completed within 8 hours, the reaction condition is mild, a high-temperature high-pressure container is not needed, the fermentation can be started to degrade the organic pollutants at normal temperature and normal pressure, the reaction site is simple, the environment-friendly biological agent can be applied to rural cities, only a basic stirring heat preservation device is needed, all strains do not contain pathogenic bacteria, the soil is regulated and repaired, the pollutants are fermented into nutrient soil after being degraded, the environment-friendly biological agent can be returned to the field, and the garbage recycling treatment is really achieved.
The above is only a specific application example of the present invention, and the protection scope of the present invention is not limited in any way. All the technical solutions formed by equivalent transformation or equivalent replacement fall within the protection scope of the present invention.
Claims (5)
1. An environment-friendly biological agent for treating organic garbage is characterized in that: the composition comprises, by weight, 8-13 parts of bacillus licheniformis powder, 10-15 parts of bacillus pumilus powder, 13-16 parts of nitrobacteria powder, 8-10 parts of lactobacillus powder, 7-10 parts of bacillus megaterium powder, 8-12 parts of bacillus subtilis powder, 9-14 parts of bacillus amyloliquefaciens powder, 5-8 parts of saccharomyces cerevisiae powder, 1-5 parts of bacillus mucilaginosus powder, 1-5 parts of soybean rhizobium powder, 2-4 parts of azotobacter chroococcum powder, 3-7 parts of pseudomonas fusca powder, 1-3 parts of lactobacillus delbrueckii, 1-3 parts of cellulase, 0.5-2 parts of ligninase, 0.01-0.05 part of 90% amino oligosaccharin and 0.05-1 part of brassinolide.
2. The method for preparing the environment-friendly biological agent for treating organic waste according to claim 1, characterized in that: the method comprises the following steps:
s1: generating flora: adding bacillus licheniformis powder, bacillus pumilus powder, nitrobacteria powder, lactobacillus powder, bacillus megaterium powder, bacillus subtilis powder, bacillus amyloliquefaciens powder, saccharomyces cerevisiae powder, bacillus mucilaginosus powder, soybean rhizobium powder, azotobacter chroococcum powder, brown high-temperature pseudomonas powder and lactobacillus delbrueckii according to the weight parts, additionally adding 10-15 parts of peptone, 10-20 parts of lactobacillus fermentation mother liquor, 1-2 parts of brown sugar and 1-2 parts of urea, and performing constant-temperature fermentation for 10-14 days, wherein the ph range is 6-8;
s2, secondary activation culture: on the basis of S1, adding 10-20 parts of wort, 1-3 parts of monohydrogenphosphate and 1-2 parts of dihydrogen phosphate into the flora, adjusting the ph to 7-7.5, adding 0.3-1 part of calcium carbonate and 0.5-1 part of calcium nitrate to further activate the microbial inoculum, and continuously culturing on a shaking table for 10-24 hours;
s3, industrial fermentation propagation: on the basis of S2, adding 5-10 parts of corn germ meal, 10-12 parts of tenebrio molitor sand and 5-8 parts of corn bran into the microbial inoculum, performing industrial fermentation propagation for 1-3 days, and completing fermentation when the concentration of strains reaches 1000 hundred million cfu/g;
s4, freeze-drying the fermentation liquor in the S3 to form powder, adding cellulase, ligninase, 90% of amino-oligosaccharin and brassinolide according to the weight parts, and drying and storing in a dark place to obtain the environment-friendly biological agent.
3. The method for preparing the environment-friendly biological agent for treating organic waste according to claim 2, characterized in that: in S1, the temperature for constant temperature fermentation is 37 ℃.
4. The method for preparing the environment-friendly biological agent for treating organic waste according to claim 2, characterized in that: in S2, the speed of the shaker was 100-150 rpm.
5. The method for preparing the environment-friendly biological agent for treating organic waste according to claim 2, characterized in that: in S3, the temperature for industrial fermentation propagation is 40 ℃, and the ph is 6.8-7.5.
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| CN113278539A (en) * | 2021-03-18 | 2021-08-20 | 惠州市通用机电设备有限公司 | Microbial inoculum for treating organic garbage and preparation and use methods thereof |
| CN113976601A (en) * | 2021-11-12 | 2022-01-28 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating kitchen waste |
| CN114015597A (en) * | 2021-11-04 | 2022-02-08 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating garden residual branch organic garbage |
| CN114058540A (en) * | 2021-11-04 | 2022-02-18 | 上海玖霖环保科技有限公司 | Environment-friendly biological agent for treating organic garbage of fruits and vegetables |
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Address after: 201600 Building 2, 117 Hulu Road, Xinbang Town, Songjiang District, Shanghai Applicant after: Shanghai Jiulin Environmental Protection Technology Co.,Ltd. Address before: No.398, Xinlu Road, Xinbang Town, Songjiang District, Shanghai, 201600 Applicant before: Shanghai Jiulin Environmental Protection Technology Co.,Ltd. |
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