CN104046363A - Method for preparing soil restoration modifier by utilization of spore fermentation wastewater - Google Patents
Method for preparing soil restoration modifier by utilization of spore fermentation wastewater Download PDFInfo
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- CN104046363A CN104046363A CN201310085536.3A CN201310085536A CN104046363A CN 104046363 A CN104046363 A CN 104046363A CN 201310085536 A CN201310085536 A CN 201310085536A CN 104046363 A CN104046363 A CN 104046363A
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Abstract
The invention provides a method for preparing a soil restoration modifier by the utilization of spore fermentation wastewater, comprising the following steps: inoculating a composite strain to a medium and carrying out anaerobic cultivation to obtain a composite microbial system; and inoculating the composite microbial system to a fermentation medium and carrying out anaerobic fermentation to obtain the soil restoration modifier. The soil restoration modifier contains a plurality of active microorganisms, enzyme, organic acid and antibiotics, can play a role in promoting a benign micro-ecological environment in crop soil, can be used to solubilize organic and inorganic nutrients, promote nutrient absorption and conversion of crops, improve granular structure of soil and simultaneously inhibit propagation of harmful pathogen. Pesticide residues in soil are decomposed to some extent. By the method, the purpose of emission reduction can be achieved, and the environment is protected. In addition, nutritional ingredients in wastewater can be fully used in the production of the soil restoration modifier. Cost and water resources are saved. The method is simple and easy to operate, has low operation cost and is easy for industrial application.
Description
Technical field
The present invention relates to a kind of method of preparing soil remediation modifying agent, relate in particular to a kind of method of utilizing gemma fermented waste fluid to prepare soil remediation modifying agent.
Background technology
Genus bacillus (Bacillaceae), a section for bacterium, can form bacillus or the coccus of gemma (statospore).Comprise bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and gemma Sarcina etc.Injurious factor resistibility is strong to external world for they, distributes wide, is present in soil, water, air and animal intestinal etc. and locates.One of genus bacillus bacillus Bacteriaceae belongs to bacterium.
Research shows, genus bacillus can produce multiple digestive ferment, helps animal digesting and assimilating nutritive substance.Genus bacillus has stronger proteolytic enzyme, amylase and lipase activity, the enzyme also with complex carbohydrates in degraded feed simultaneously, as polygalacturonase, dextranase, cellulase etc., these endonuclease capables destroy the cell walls of plant feed cell, impel the nutritive substance of cell to discharge, and can eliminate the antinutritional factor in feed, reduce the obstacle that antinutritional factor is utilized animal digestion.Therefore, feeding genus bacillus has broad application prospects in animal cultivation.
But in producing the fermented waste fluid that feeding genus bacillus produces, there is ammonia nitrogen that fermentation produces, phosphorus, multiple enzyme, residual fermention medium and the materials such as gemma of a small amount of dormant state, waste liquid COD value is in 1500mg/L left and right, can not utilize these materials, fermented abandoned liquid directly discharge can cause body eutrophication and environmental pollution.But the cultivation this waste liquid for other bacterial classifications, can make full use of residue nutritive ingredient wherein, is prepared into product innovation simultaneously.As CN101671644A provides a kind of method of utilizing gemma fermented waste fluid to prepare breeding environment scavenging agent; gemma fermented waste fluid and composite bacteria sealed fermenting are obtained to Water body cleansing agent; the method can not only reach the object of reduction of discharging; protection of the environment; and make full use of nutritive ingredient in waste liquid for the production of breeding environment modifying agent, cost-saving and water resources.And for example CN102334589A discloses a kind of method with preparing fermented feed from waste bacillus fermentation liquor; the method utilizes fermentation of bacillus waste liquid to be used for cultivating lactic acid bacteria culturers as nutrient solution, thereby be conducive to protection of the environment, solves discharging of waste liquid produced pollution problem.The present invention utilizes fermentation of bacillus waste liquid to substitute the water of productive use in fermented feed, can reach water-saving object.
Genus bacillus also can be used as bio-feritlizer, when acting on crop or soil, can be at crop rhizosphere or Colonization inside plants, and play specific fertilizer effect.At present; microbial fertilizer is at culture fertility; improve chemical fertilizer utilization ratio; suppress the absorption of farm crop to nitric nitrogen, heavy metal, agricultural chemicals; purify and rehabilitating soil, reduce corps diseases and occur, promote the utilization of becoming thoroughly decomposed of agricultural crop straw and municipal wastes; protection of the environment, and improve the aspects such as crop products quality and food safety and shown irreplaceable effect.Therefore can consider gemma fermented waste fluid for improveing soil, but not yet develop at present a kind of effective method.
Summary of the invention
The object of the invention is to address the above problem, a kind of method of utilizing gemma fermented waste fluid to prepare soil remediation modifying agent is provided.
First object of the present invention is to provide a kind of method of utilizing gemma fermented waste fluid to prepare soil remediation modifying agent, specifically comprises the following steps:
Step 1, is seeded in composite bacteria in substratum, and anaerobism is cultivated, and obtains composite microbial system; Wherein, described composite bacteria comprises two or more in Rhodopseudomonas palustris, plant lactobacillus, streptococcus uberis and saccharomyces cerevisiae; The weight ratio of composite bacteria and substratum is (0.5-10): (90-99.5);
Step 2, is seeded in the composite microbial system obtaining in step 1 in fermention medium, and anaerobically fermenting obtains soil remediation modifying agent; Wherein, composite microbial system accounts for the 10-20wt% of the total mass of composite microbial system and fermention medium; Described fermention medium comprises substrate carrier 20-50wt%, gemma fermented waste fluid 10-15wt%, industrial culture medium 10-20wt%, soil extract 2-5wt%, growth factor solution 0.5-1wt% and excess water.
Preferably, in step 1, anaerobic fermentation conditions is: anaerobically fermenting 100-200h at 20-40 ℃.
Further preferably, in step 1, anaerobic fermentation conditions is: anaerobically fermenting 120-130h at 30-37 ℃.
Preferably, in step 1, the weight ratio of composite bacteria and substratum is (1-2): (98-99).
Preferably, described in step 1, composite bacteria comprises 0.5 * 10
9cfu/ml Rhodopseudomonas palustris 5-7wt%, 5 * 10
9cfu/ml plant lactobacillus 35-40wt%, 5 * 10
9cfu/ml streptococcus uberis 40-45wt% and 2.5 * 10
9two or more in cfu/ml saccharomyces cerevisiae 20-25wt%.
Preferably, substratum described in step 1 comprises the water of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 3-3.5wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt%, soil extract 10-15wt%, gemma fermented waste fluid 5-7wt% and surplus.
Preferably, described growth factor solution is the aqueous solution that comprises vitamin H 2-2.3wt%, vitamin B12-15wt%.
Further preferably, described growth factor solution is comprised of the aqueous solution of vitamin H 2-2.3wt%, vitamin B12-15wt%.
Preferably, the pH value of substratum described in step 1 is 5-8,6-7 more preferably, more preferably 6.5.
Preferably, in step 2, anaerobic fermentation conditions is: anaerobically fermenting 50-120h at 20-40 ℃.
Further preferably, in step 2, anaerobic fermentation conditions is: anaerobically fermenting 72-90h at 30-35 ℃.
Preferably, described gemma fermented waste fluid is centrifugal by producing the fermented waste fluid containing gemma that gemma microorganism feed addictive produces, and gets supernatant and makes.
Wherein, the fermented waste fluid centrifugal condition containing gemma is preferably the centrifugal 3-3.5h of 6000-7000rpm.
Preferably, the carrier of substrate described in step 2 is selected from least one in blue or green Kang, peanut hull meal, corn stalk powder and the assorted dregs of rice.
Preferably, industrial culture medium described in step 2 is one or more the mixture in molasses, W-Gum, soybean cake powder, magnesium sulfate, potassium primary phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride and sodium-chlor.
Preferably, described soil extract is the liquid that aseptic soil leaches after 30-36 hour soaks.
Preferably, provided by the inventionly utilize the method that gemma fermented waste fluid is prepared soil remediation modifying agent also to comprise post-processing step: the tunning drying and crushing that step 2 is obtained, sieve.
Second object of the present invention is to provide a kind of soil remediation modifying agent of preparing according to the above-mentioned method of utilizing gemma fermented waste fluid to prepare soil remediation modifying agent.
The 3rd object of the present invention is to provide a kind of soil remediation modification method, utilizes gemma fermented waste fluid to prepare soil remediation modifying agent, uses described soil remediation modifying agent to carry out soil remediation improvement.
Method provided by the invention is inoculated in composite microbial system in the substratum of the composition such as mixings of using fermented waste fluid, soil extract, somatomedin (trace element) solution and prepares soil remediation modifying agent, it contains various active microorganism, enzyme, organic acid and antibiotics, can play promoter action to optimum micro-ecological environment in crop soil, make organic and inorganic nutrients solubilization, promote crop to transform the absorption of nutrient, improve soil aggregate, suppress growing of harmful pathogenic bacteria simultaneously, decompose to a certain extent the residual harm of agriculture in soil.
Method of the present invention can not only reach the object of reduction of discharging; protection of the environment; and make full use of nutritive ingredient in waste liquid for the production of soil remediation modifying agent, cost-saving and water resources, have easy to operation, running cost is low, be easy to the outstanding features such as industrial applications.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described, to understand better the present invention.
Rhodopseudomonas palustris in embodiment, Rhodopseudomonas palustris, purchased from Chinese common micro-organisms culture presevation administrative center, numbering CGMCC1.2349; Described plant lactobacillus, Lactobacillus plantarum, purchased from Chinese agriculture microbial strains preservation administrative center, numbering ACCC11118; Described streptococcus uberis, Streptococcus lactis, purchased from microbial collection center, Sichuan Province, numbering SCTCC100731; Described saccharomyces cerevisiae, Saccharomyces cerevisiae, purchased from Chinese agriculture microbial strains preservation administrative center, numbering ACCC20065.
Embodiment 1
The first step, accesses composite bacteria in substratum, under 30 ℃ of conditions, cultivates 120h, obtains composite microbial system.
Wherein, described composite bacteria comprises 0.5 * 10
9cfu/ml Rhodopseudomonas palustris 5wt%, 5 * 10
9cfu/ml plant lactobacillus 35wt%, 5 * 10
9cfu/ml streptococcus uberis 40wt% and 2.5 * 10
9cfu/ml saccharomyces cerevisiae 20wt%.The weight ratio of composite bacteria and substratum is 1:99.
Wherein, described substratum is comprised the aqueous solution of vitamin H 2wt%, vitamin B12 wt% by molasses 1wt%, peptone 0.4wt%, yeast extract paste 1wt%, calcium chloride 0.1wt%, sodium-chlor 0.2wt%, ferric sulfate 3.0wt%, magnesium sulfate 0.02wt%, growth factor solution 1wt%(), the water of soil extract 10wt% and surplus forms, pH6.5.
Second step, the composite microbial system that the first step is obtained is seeded in fermention medium, and after evenly mixing, at 33.3 ℃, under the condition of agitator speed 60r/min, anaerobically fermenting is 72 hours.Wherein composite microbial system accounts for the 15wt% of the total mass of composite microbial system and fermention medium.Fermention medium is comprised of substrate carrier 30 as one kind t%, gemma fermented waste fluid 10wt%, industrial culture medium 15wt%, soil extract 2-5wt%, growth factor solution 1wt% and excess water.
Wherein, described gemma fermented waste fluid,, is got supernatant and makes with the centrifugal 3h of 6000rpm by the fermented waste fluid containing gemma that production gemma microorganism feed addictive is produced.
Wherein, the liquid that the aseptic soil in described soil extract position leaches after 30 hours soak.
Wherein, described growth factor solution, is comprised of the aqueous solution of vitamin H 2wt%, vitamin B12 wt%.
Wherein, described industrial culture medium is by the compositions of mixtures of molasses, W-Gum, soybean cake powder, magnesium sulfate, potassium primary phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, sodium-chlor.
Wherein, described substrate carrier is by the compositions of mixtures of blue or green Kang, peanut hull meal, corn stalk powder.
The 3rd step, by gained tunning drying and crushing, crosses 50 mesh sieves, obtains soil remediation modifying agent.Embodiment 2
The first step, accesses composite bacteria in substratum, under 37 ℃ of conditions, cultivates 130h, obtains composite microbial system.
Wherein, described composite bacteria comprises 0.5 * 10
9cfu/ml Rhodopseudomonas palustris 7wt%, 5 * 10
9cfu/ml plant lactobacillus 45wt%, 5 * 10
9cfu/ml streptococcus uberis 45wt% and 2.5 * 10
9two or more in cfu/ml saccharomyces cerevisiae 25wt%.The weight ratio of composite bacteria and substratum is 2:98.
Wherein, described substratum is comprised the aqueous solution of vitamin H 2.3wt%, vitamin B15 wt% by molasses 1.2wt%, peptone 0.6wt%, yeast extract paste 1.2wt%, calcium chloride 0.15wt%, sodium-chlor 0.25wt%, ferric sulfate 3.5wt%, magnesium sulfate 0.03wt%, growth factor solution 1.2wt%(), the water of soil extract 13wt% and surplus forms, pH6.5.
Second step, the composite microbial system mother liquor that the first step is obtained accesses in the large-scale fermention medium being comprised of substrate carrier 30 as one kind t%, gemma fermented waste fluid 10wt%, industrial culture medium 15wt%, soil extract 2-5wt%, growth factor solution 1wt% excess water with the ratio of 15wt%, after evenly mixing, at 33.3 ℃, under the condition of agitator speed 60r/min, anaerobically fermenting is 72 hours.
Wherein, described gemma fermented waste fluid,, is got supernatant and makes with the centrifugal 3.5h of 7000rpm by the gemma fermented waste fluid that production gemma microorganism feed addictive is produced.
Wherein, the liquid that the aseptic soil in described soil extract position leaches after 36 hours soak.
Wherein, described growth factor solution, is comprised of the aqueous solution of vitamin H 2wt%, vitamin B15 wt%.
Wherein, described industrial culture medium is by the compositions of mixtures of molasses, W-Gum, soybean cake powder, magnesium sulfate, potassium primary phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride, sodium-chlor.
Wherein, described substrate carrier is by the compositions of mixtures of blue or green Kang, peanut hull meal, corn stalk powder.
The 3rd step, by gained tunning drying and crushing, crosses 50 mesh sieves, obtains soil remediation modifying agent.
Above specific embodiments of the invention be have been described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and alternative also all among category of the present invention.Therefore, equalization conversion and the modification done without departing from the spirit and scope of the invention, all should contain within the scope of the invention.
Claims (10)
1. utilize gemma fermented waste fluid to prepare a method for soil remediation modifying agent, it is characterized in that, comprise the following steps:
Step 1, is seeded in composite bacteria in substratum, and anaerobism is cultivated, and obtains composite microbial system; Wherein, described composite bacteria comprises two or more in Rhodopseudomonas palustris, plant lactobacillus, streptococcus uberis and saccharomyces cerevisiae; The weight ratio of composite bacteria and substratum is (0.5-10): (90-99.5);
Step 2, is seeded in the composite microbial system obtaining in step 1 in fermention medium, and anaerobically fermenting obtains soil remediation modifying agent; Wherein, composite microbial system accounts for the 10-20wt% of the total mass of composite microbial system and fermention medium; Described fermention medium comprises substrate carrier 20-50wt%, gemma fermented waste fluid 10-15wt%, industrial culture medium 10-20wt%, soil extract 2-5wt%, growth factor solution 0.5-1wt% and excess water.
2. method according to claim 1, is characterized in that, in step 1, anaerobic fermentation conditions is: anaerobically fermenting 100-200h at 20-40 ℃.
3. method according to claim 1, is characterized in that, in step 2, anaerobic fermentation conditions is: anaerobically fermenting 50-120h at 20-40 ℃.
4. method according to claim 1, is characterized in that, described in step 1, composite bacteria comprises 0.5 * 10
9cfu/ml Rhodopseudomonas palustris 5-7wt%, 5 * 10
9cfu/ml plant lactobacillus 35-40wt%, 5 * 10
9cfu/ml streptococcus uberis 40-45wt% and 2.5 * 10
9two or more in cfu/ml saccharomyces cerevisiae 20-25wt%.
5. method according to claim 1, it is characterized in that, substratum described in step 1 comprises the water of molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract paste 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium-chlor 0.2-0.25wt%, ferric sulfate 3-3.5wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt%, soil extract 10-15wt%, gemma fermented waste fluid 5-7wt% and surplus.
6. method according to claim 1 or 5, is characterized in that, described growth factor solution is the aqueous solution that comprises vitamin H 2-2.3wt%, vitamin B12-15wt%.
7. preparation method according to claim 1, it is characterized in that, industrial culture medium described in step 2 is one or more the mixture in molasses, W-Gum, soybean cake powder, magnesium sulfate, potassium primary phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride and sodium-chlor.
8. method according to claim 1 or 5, is characterized in that, described gemma fermented waste fluid is centrifugal by producing the fermented waste fluid containing gemma that gemma microorganism feed addictive produces, and gets supernatant and makes.
One kind in accordance with the method for claim 1 preparation soil remediation modifying agent.
10. a soil remediation modification method, comprises according to method described in claim 1, utilizes gemma fermented waste fluid to prepare soil remediation modifying agent, uses described soil remediation modifying agent to carry out soil remediation improvement.
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| CN105331556A (en) * | 2015-11-12 | 2016-02-17 | 河南兴禾生物科技有限公司 | Composite micro-ecological preparation and preparation method and application thereof |
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| CN105062493A (en) * | 2015-07-30 | 2015-11-18 | 江西新太阳生态肥业有限公司 | Preparation method of microbial soil amendment |
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| CN105967889A (en) * | 2016-06-07 | 2016-09-28 | 内蒙古阜丰生物科技有限公司 | Soil conditioner containing glutamic acid fermentation mother liquor |
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| CN106010561A (en) * | 2016-06-08 | 2016-10-12 | 内蒙古阜丰生物科技有限公司 | Soil conditioning agent containing threonine fermentation waste |
| CN106010561B (en) * | 2016-06-08 | 2019-06-21 | 内蒙古阜丰生物科技有限公司 | A kind of soil conditioner containing Threonine Fermentation waste |
| CN108184836A (en) * | 2017-11-23 | 2018-06-22 | 北京联合大学 | A kind of combined fermentation object for accelerating crops degrading pesticide residue |
| CN112522155A (en) * | 2020-11-17 | 2021-03-19 | 上海圣珑环境修复材料有限公司 | Bacillus licheniformis and application thereof |
| CN112522155B (en) * | 2020-11-17 | 2021-09-21 | 上海圣珑环境修复材料有限公司 | Bacillus licheniformis and application thereof |
| CN112961796A (en) * | 2021-01-29 | 2021-06-15 | 湖北华扬科技发展有限公司 | Method for producing clostridium butyricum by fermenting enterococcus faecium fermentation waste liquid |
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