CN103305225A - Method for preparing soil remediation conditioner by using spore fermentative waste - Google Patents
Method for preparing soil remediation conditioner by using spore fermentative waste Download PDFInfo
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- CN103305225A CN103305225A CN2013102173284A CN201310217328A CN103305225A CN 103305225 A CN103305225 A CN 103305225A CN 2013102173284 A CN2013102173284 A CN 2013102173284A CN 201310217328 A CN201310217328 A CN 201310217328A CN 103305225 A CN103305225 A CN 103305225A
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Abstract
The invention provides a method for preparing soil remediation conditioner by using spore fermentative waste. The method comprises the following steps: inoculating pseudomonas pseudoacaligenes into a culture medium, anaerobically culturing to obtain the pseudomonas pseudoacaligenes liquor, inoculating the pseudomonas pseudoacaligenes liquor into a fermentation culture medium, anaerobically culturing to obtain the soil remediation conditioner. The obtained remediation conditioner comprises a plurality of active microorganisms, enzymes, organic acids and antibiotics, and is capable of promoting the good micro-ecological environment in the crop soil, solubilizing the organic and inorganic nutrients, promoting the nutrient adsorption and conversion of the crop, improving the soil granular structure as well as inhibiting the breeding of the harmful pathogenic bacteria, and the pesticide residue harm in the soil is dissolved to a certain degree. The method provided by the invention is capable of reducing emission and protecting environment, and the nutrient component in the waste liquid can be sufficiently used for producing the soil remediation conditioner, the cost and water resource are saved, the operation is convenient, simple and easy, the operation cost is low, and the industrial application can be easily realized.
Description
Technical field
The present invention relates to a kind of method for preparing the soil remediation modifying agent, relate in particular to a kind of method of utilizing the gemma fermented waste fluid to prepare the soil remediation modifying agent.
Background technology
Genus bacillus (Bacillaceae), the section of bacterium can form bacillus or the coccus of gemma (statospore).Comprise bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and gemma Sarcina etc.The injurious factor resistibility is strong to external world for they, and it is wide to distribute, and is present in soil, water, air and animal intestinal etc. and locates.One of genus bacillus bacillus Bacteriaceae belongs to bacterium.
Studies show that genus bacillus can produce multiple digestive ferment, help animal digesting and assimilating nutritive substance.Genus bacillus has stronger proteolytic enzyme, amylase and lipase activity, the enzyme that also has complex carbohydrates in the degraded feed simultaneously, as polygalacturonase, dextranase, cellulase etc., these endonuclease capables destroy the cell walls of plant feed cell, impel the nutritive substance of cell to discharge, and can eliminate antinutritional factor in the feed, reduce the obstacle that antinutritional factor is utilized animal digestion.Therefore, feeding genus bacillus has broad application prospects in animal cultivation.
But in producing the fermented waste fluid that feeding genus bacillus produces, there are ammonia nitrogen that fermentation produces, phosphorus, multiple enzyme, residual fermention medium and the materials such as gemma of a small amount of dormant state, waste liquid COD value is about 1500mg/L, can not utilize these materials, fermented abandoned liquid directly discharging can cause body eutrophication and environmental pollution.But this waste liquid is used for other culture of strains, can takes full advantage of residue nutritive ingredient wherein, be prepared into product innovation simultaneously.Provide a kind of method of utilizing the gemma fermented waste fluid to prepare the breeding environment scavenging agent as CN101671644A; gemma fermented waste fluid and composite bacteria sealed fermenting are obtained the water body purification agent; this method can not only reach the purpose of reduction of discharging; the protection environment; and take full advantage of the production that nutritive ingredient in the waste liquid is used for the breeding environment modifying agent, save cost and water resources.And for example CN102334589A discloses a kind of method for preparing fermented feed with the fermentation of bacillus waste liquid; this method utilizes the fermentation of bacillus waste liquid to be used for cultivating lactic acid bacteria culturers as nutrient solution, thereby is conducive to protect environment to solve discharging of waste liquid produced pollution problem.The present invention utilizes the water of productive use in the alternative fermented feed of fermentation of bacillus waste liquid, can reach water-saving purpose.
Genus bacillus also can be used as bio-feritlizer, when acting on crop or soil, can at crop rhizosphere or body is decided at the higher level but not officially announced grows, and plays the specific fertilizer effect.At present; microbial fertilizer is at culture fertility; improve chemical fertilizer utilization ratio; suppress farm crop to the absorption of nitric nitrogen, heavy metal, agricultural chemicals; purify and rehabilitating soil, reduce corps diseases and take place, promote the utilization of becoming thoroughly decomposed of agricultural crop straw and municipal wastes; the protection environment, and aspects such as raising crop products quality and food safety have shown irreplaceable effect.Therefore can consider the gemma fermented waste fluid is used for improving the soil, but still untappedly at present go out a kind of efficient ways.
Summary of the invention
The objective of the invention is to address the above problem, a kind of method of utilizing the gemma fermented waste fluid to prepare the soil remediation modifying agent is provided.
First purpose of the present invention provides a kind of method of utilizing the gemma fermented waste fluid to prepare the soil remediation modifying agent, specifically may further comprise the steps:
Step 1 is seeded in pseudomonas in the substratum, and anaerobically fermenting obtains the sub-liquid of pseudomonas; Wherein, pseudomonas accounts for the 0.5-5wt% of the total mass of pseudomonas and substratum;
Step 2 is seeded in the sub-liquid of the pseudomonas that obtains in the step 1 in the fermention medium, and anaerobically fermenting obtains the soil remediation modifying agent; Wherein, the sub-liquid of pseudomonas accounts for the 0.5-5wt% of the total mass of the sub-liquid of pseudomonas and fermention medium; Described fermention medium comprises solid state substrate and gemma fermented waste fluid, and the mass ratio of solid state substrate and gemma fermented waste fluid is 1:(1-5).
Wherein, the anaerobically fermenting temperature is 20-60 ℃ in the step 1, is preferably 25-55 ℃, is preferably 30-50 ℃, more preferably 30-45 ℃, and more preferably 35-40 ℃.
Wherein, the anaerobically fermenting time is 10-60h in the step 1, is preferably 15-55h, is preferably 20-50h, more preferably 24-48h, more preferably 30-40h.
Wherein, the anaerobically fermenting temperature is 20-60 ℃ in the step 2, is preferably 25-55 ℃, is preferably 30-50 ℃, more preferably 30-45 ℃, and more preferably 35-40 ℃.
Wherein, the anaerobically fermenting time is 50-150h in the step 2, is preferably 60-130h, is preferably 72-120h, more preferably 80-100h.
Wherein, pseudomonas described in the step 1 is selected from the following type pseudomonas one or more in the step 1: Pseudomonas amygdali, hazel look pseudomonas (P.avellanae), Pseudomonas caricapapayae (P.caricapapayae), Pseudomonas cichorii (P.cichorii), Fructus Fici pseudomonas (P.ficuserectae), Pseudomonas meliae (P.meliae), Sa Shi pseudomonas (P.savastanoi), pseudomonas syringae (P.syringae), Pseudomonas viridiflava (P.viridiflava), Pseudomonas aurantica (P.aurantiaca), Pseudomonas chlororaphis (P.chlororaphis), mould real pseudomonas (P.fragi), Longde pseudomonas (P.1undensis), Pseudomonas taetrolens (P.taetrolens), produce nitrogen pseudomonas (P.azotoformans), P.cedrella, shrinkage pseudomonas (P.corrugata), Pseudomonas fluorescens (P.Zuorescens), P.gessardii, P.libaniensis, edge pseudomonas (P.marginalis), P.migulae, Pseudomonas mucidolens (P.mucidolens), P.orientalis, Luo Shi pseudomonas (P.rhodesiae), Pseudomonas pseudoflava (P.synxantha), pseudomonas thomasii (P.tolaasii), Wei Long Shi pseudomonas (P.veronii), yellowish-brown pseudomonas (P.fulva), Meng Shi pseudomonas (P.monteilii), P.mosselii, pseudomonas putida (P.putida), Bali Ali pseudomonas (P.balearica), Pseudomonas stutzeri (P.stutzeri), Pseudomonas anguilliseptica (P.anguilliseptica), Pseudomonas alcaligenes (P.alcaligenes), pseudomonas citronelloalis (P.citronellolis), flavescence pseudomonas (P.flavescens), multi-door Sa pseudomonas (P.mendocina), Pseudomonas nitroreducens (P.nitroreducens), Pseudomonas oleovorans (P.oleovarans), pseudomonas pseudoalcaligenes (P.pseudoalcaligenes), food tree pseudomonas (P.resinovorans), Pseudomonas straminea (P.straminae), Pseuomonas denitrifican (P.denitrificans), pore-forming protein pseudomonas (P.pertucinogena), agaric pseudomonas (P.agarici), P.issenii, Pseudomonas asplenii (P.asplenii), Pseudomonas fuscovaginae (P.fuscowginae).
Preferably, substratum described in the step 1 comprises molasses 1-5wt%, agar powder 1-3wt% and surplus gemma fermented waste fluid.
Preferably, substratum described in the step 1 is used for the cultivation of pseudomonas behind 110-121 ℃ of sterilization 15-30min.
Preferably, the fermented waste fluid that contains gemma that described gemma fermented waste fluid is produced by production gemma microorganism feed addictive is centrifugal, gets supernatant and makes.
Wherein, the fermented waste fluid centrifugal condition that contains gemma is preferably the centrifugal 3-3.5h of 6000-7000rpm.
Preferably, the method of the invention also comprises preceding culturing step: with the pseudomonas bacterial classification inoculation on special culture media, 20-60 ℃ (is preferably 25-55 ℃, is preferably 30-50 ℃, more preferably 30-45 ℃, more preferably 35-40 ℃) descend cultivation 10-60h(to be preferably 15-55h, be preferably 20-50h, more preferably 24-48h, more preferably 30-40h), make the pseudomonas mother liquor, gained pseudomonas mother liquor is used for step 1.
Wherein, special culture media described in the preceding culturing step comprises peptone 1-2wt%, extractum carnis 1-2wt%, yeast extract paste 0.5-1wt%, hydrogen sulfate two ammonium 0.2-0.4wt%, glucose 2-3wt%, sodium acetate 0.2-0.4wt%, tween 80 0.1-0.3wt%, dipotassium hydrogen phosphate 0.2-0.5wt%, sal epsom 0.05-0.06wt%, four anhydrous manganese 0.025-0.03wt%, seven ferric sulfate hydrate 0.025-0.03wt%, calcium carbonate 0.1-0.2wt% and excess water.
Preferably, special culture media described in the preceding culturing step is used for the cultivation of pseudomonas behind 115-121 ℃ of sterilization 15-30min.
Preferably, the method for the invention also comprises post-processing step: with step 2 gained soil remediation modifying agent drying and/or pulverizing.
Preferably, step 2 gained soil remediation modifying agent is being dried to moisture less than 15%.
Wherein, solid state substrate described in the step 2 often is macromolecular cpd, as starch, Mierocrystalline cellulose, hemicellulose, pectin, xylogen, protein and lipid etc.
Second purpose of the present invention provides a kind of soil remediation modifying agent for preparing according to the above-mentioned method of utilizing the gemma fermented waste fluid to prepare the soil remediation modifying agent.
The 3rd purpose of the present invention provides a kind of soil remediation modification method, utilizes the gemma fermented waste fluid to prepare the soil remediation modifying agent, uses described soil remediation modifying agent to carry out the soil remediation improvement.
The 4th purpose of the present invention provides a kind of fermention medium, comprise solid state substrate and gemma fermented waste fluid, and the mass ratio of solid state substrate and gemma fermented waste fluid is 1:(1-5).
Method provided by the invention is inoculated in pseudomonas in the substratum of compositions such as mixing of using fermented waste fluid and solid state substrate and prepares the soil remediation modifying agent, it contains various active microorganism, enzyme, organic acid and antibiotics, can play promoter action to optimum micro-ecological environment in the crop soil, make organic and inorganic nutrients can dissolve, promote that crop transforms nutrient absorbing, improve soil aggregate, suppress growing of harmful pathogenic bacteria simultaneously, decompose the agricultural residual harm in the soil to a certain extent.
Method of the present invention can not only reach the purpose of reduction of discharging; the protection environment; and take full advantage of the production that nutritive ingredient in the waste liquid is used for the soil remediation modifying agent, save cost and water resources, have easy to operation, running cost is low, be easy to outstanding feature such as industrial applications.
Embodiment
The invention will be further described below in conjunction with specific embodiment, to understand the present invention better.
Pseudomonas among the embodiment is selected from one or more in the following type pseudomonas: Pseudomonas amygdali, hazel look pseudomonas (P.avellanae), Pseudomonas caricapapayae (P.caricapapayae), Pseudomonas cichorii (P.cichorii), Fructus Fici pseudomonas (P.ficuserectae), Pseudomonas meliae (P.meliae), Sa Shi pseudomonas (P.savastanoi), pseudomonas syringae (P.syringae), Pseudomonas viridiflava (P.viridiflava), Pseudomonas aurantica (P.aurantiaca), Pseudomonas chlororaphis (P.chlororaphis), mould real pseudomonas (P.fragi), Longde pseudomonas (P.1undensis), Pseudomonas taetrolens (P.taetrolens), produce nitrogen pseudomonas (P.azotoformans), P.cedrella, shrinkage pseudomonas (P.corrugata), Pseudomonas fluorescens (P.Zuorescens), P.gessardii, P.libaniensis, edge pseudomonas (P.marginalis), P.migulae, Pseudomonas mucidolens (P.mucidolens), P.orientalis, Luo Shi pseudomonas (P.rhodesiae), Pseudomonas pseudoflava (P.synxantha), pseudomonas thomasii (P.tolaasii), Wei Long Shi pseudomonas (P.veronii), yellowish-brown pseudomonas (P.fulva), Meng Shi pseudomonas (P.monteilii), P.mosselii, pseudomonas putida (P.putida), Bali Ali pseudomonas (P.balearica), Pseudomonas stutzeri (P.stutzeri), Pseudomonas anguilliseptica (P.anguilliseptica), Pseudomonas alcaligenes (P.alcaligenes), pseudomonas citronelloalis (P.citronellolis), flavescence pseudomonas (P.flavescens), multi-door Sa pseudomonas (P.mendocina), Pseudomonas nitroreducens (P.nitroreducens), Pseudomonas oleovorans (P.oleovarans), pseudomonas pseudoalcaligenes (P.pseudoalcaligenes), food tree pseudomonas (P.resinovorans), Pseudomonas straminea (P.straminae), Pseuomonas denitrifican (P.denitrificans), pore-forming protein pseudomonas (P.pertucinogena), agaric pseudomonas (P.agarici), P.issenii, Pseudomonas asplenii (P.asplenii), Pseudomonas fuscovaginae (P.fuscowginae).
Embodiment 1
The first step on special culture media, is cultivated the pseudomonas bacterial classification inoculation down 48h, is made the pseudomonas mother liquor for 37 ℃.
Wherein, described special culture media comprises peptone 2wt%, extractum carnis 2wt%, yeast extract paste 1wt%, hydrogen sulfate two ammonium 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, tween 80 0.2wt%, dipotassium hydrogen phosphate 0.2wt%, sal epsom 0.05wt%, four anhydrous manganese 0.025wt%, seven ferric sulfate hydrate 0.025wt%, calcium carbonate 0.1wt% and excess water, and 120 ℃ of sterilization 20min.
Second step was seeded in the pseudomonas mother liquor that makes in the substratum, and 37 ℃ of following anaerobically fermenting 48h obtain the sub-liquid of pseudomonas.Wherein, pseudomonas accounts for the 2.5wt% of the total mass of pseudomonas and substratum; Described substratum comprises molasses 5wt%, agar powder 3wt% and surplus gemma fermented waste fluid, and 120 ℃ of sterilization 20min.
The 3rd step was seeded in the sub-liquid of the pseudomonas that obtains in the fermention medium, 37 ℃ of following anaerobically fermenting 80h, and wherein, the sub-liquid of pseudomonas accounts for the 2.5wt% of the total mass of the sub-liquid of pseudomonas and fermention medium; Described fermention medium comprises solid state substrate and gemma fermented waste fluid, and 120 ℃ of sterilization 20min, and wherein, the mass ratio of solid state substrate and gemma fermented waste fluid is 1:3.
Wherein, described gemma fermented waste fluid by producing the fermented waste fluid that contains gemma that the gemma microorganism feed addictive produces with the centrifugal 3h of 6000rpm, is got supernatant and is made.Described solid state substrate is macromolecular cpd, as in starch, Mierocrystalline cellulose, hemicellulose, pectin, xylogen, protein and the lipid etc. one or more.
The 4th step, with 50 ℃ of gained tunnings dry to moisture less than 15%, pulverize and to obtain soil improvement agent.Embodiment 2
The first step on special culture media, is cultivated the pseudomonas bacterial classification inoculation down 36h, is made the pseudomonas mother liquor for 37 ℃.
Wherein, described special culture media comprises peptone 1wt%, extractum carnis 1wt%, yeast extract paste 0.5wt%, hydrogen sulfate two ammonium 0.4wt%, glucose 2wt%, sodium acetate 0.4wt%, tween 80 0.2wt%, dipotassium hydrogen phosphate 0.2wt%, sal epsom 0.06wt%, four anhydrous manganese 0.03wt%, seven ferric sulfate hydrate 0.025wt%, calcium carbonate 0.1wt% and excess water, and 120 ℃ of sterilization 20min.
Second step was seeded in the pseudomonas mother liquor that makes in the substratum, and 37 ℃ of following anaerobically fermenting 36h obtain the sub-liquid of pseudomonas.Wherein, pseudomonas accounts for the 0.5wt% of the total mass of pseudomonas and substratum; Described substratum comprises molasses 2wt%, agar powder 1wt% and surplus gemma fermented waste fluid, and 120 ℃ of sterilization 20min.
The 3rd step was seeded in the sub-liquid of the pseudomonas that obtains in the fermention medium, 37 ℃ of following anaerobically fermenting 120h, and wherein, the sub-liquid of pseudomonas accounts for the 5wt% of the total mass of the sub-liquid of pseudomonas and fermention medium; Described fermention medium comprises solid state substrate and gemma fermented waste fluid, and 120 ℃ of sterilization 20min, and wherein, the mass ratio of solid state substrate and gemma fermented waste fluid is 1:5.
Wherein, described gemma fermented waste fluid by producing the fermented waste fluid that contains gemma that the gemma microorganism feed addictive produces with the centrifugal 3h of 6000rpm, is got supernatant and is made.Described solid state substrate is macromolecular cpd, as in starch, Mierocrystalline cellulose, hemicellulose, pectin, xylogen, protein and the lipid etc. one or more.
The 4th step, with 40 ℃ of gained tunnings dry to moisture less than 15%, pulverize and to obtain soil improvement agent.
Pseudomonas can produce plurality of enzymes, organic acid and antibiotics, is used for the degradation property biological restoration of soil, regulates soil pH, promotes the improvement of soil micro-ecosystem environment.
More than specific embodiments of the invention are described in detail, but it is just as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and substituting also all among category of the present invention.Therefore, not breaking away from impartial conversion and the modification of doing under the spirit and scope of the present invention, all should contain within the scope of the invention.
Claims (10)
1. a method of utilizing the gemma fermented waste fluid to prepare the soil remediation modifying agent is characterized in that, may further comprise the steps:
Step 1 is seeded in pseudomonas in the substratum, and anaerobically fermenting obtains the sub-liquid of pseudomonas; Wherein, pseudomonas accounts for the 0.5-5wt% of the total mass of pseudomonas and substratum;
Step 2 is seeded in the sub-liquid of the pseudomonas that obtains in the step 1 in the fermention medium, and anaerobically fermenting obtains the soil remediation modifying agent; Wherein, the sub-liquid of pseudomonas accounts for the 0.5-5wt% of the total mass of the sub-liquid of pseudomonas and fermention medium; Described fermention medium comprises solid state substrate and gemma fermented waste fluid, and the mass ratio of solid state substrate and gemma fermented waste fluid is 1:(1-5).
2. method according to claim 1 is characterized in that, the anaerobically fermenting condition is in the step 1: 20-60 ℃ of following anaerobically fermenting 10-60h.
3. method according to claim 1 is characterized in that, the anaerobically fermenting condition is in the step 2: 20-60 ℃ of following anaerobically fermenting 50-150h.
4. method according to claim 1 is characterized in that, substratum described in the step 1 comprises molasses 1-5wt%, agar powder 1-3wt% and surplus gemma fermented waste fluid.
5. according to claim 1 or 4 described methods, it is characterized in that the fermented waste fluid that contains gemma that described gemma fermented waste fluid is produced by production gemma microorganism feed addictive is centrifugal, gets supernatant and makes.
6. method according to claim 1 is characterized in that, also comprises preceding culturing step: the pseudomonas bacterial classification inoculation on special culture media, is cultivated 10-60h down for 20-60 ℃, make the pseudomonas mother liquor, gained pseudomonas mother liquor is used for step 1.
7. method according to claim 6, it is characterized in that described special culture media comprises peptone 1-2wt%, extractum carnis 1-2wt%, yeast extract paste 0.5-1wt%, hydrogen sulfate two ammonium 0.2-0.4wt%, glucose 2-3wt%, sodium acetate 0.2-0.4wt%, tween 80 0.1-0.3wt%, dipotassium hydrogen phosphate 0.2-0.5wt%, sal epsom 0.05-0.06wt%, four anhydrous manganese 0.025-0.03wt%, seven ferric sulfate hydrate 0.025-0.03wt%, calcium carbonate 0.1-0.2wt% and excess water.
One kind in accordance with the method for claim 1 the preparation the soil remediation modifying agent.
9. a soil remediation modification method comprises according to the described method of claim 1, utilizes the gemma fermented waste fluid to prepare the soil remediation modifying agent, uses described soil remediation modifying agent to carry out the soil remediation improvement.
10. a fermention medium is characterized in that, comprises solid state substrate and gemma fermented waste fluid, and the mass ratio of solid state substrate and gemma fermented waste fluid is 1:(1-5).
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103664254A (en) * | 2013-12-10 | 2014-03-26 | 上海师范大学 | Preparation method of compound microbial fertilizer |
| CN110615721A (en) * | 2018-06-20 | 2019-12-27 | 四川省农业科学院生物技术核技术研究所 | Soil biological denitrifier and preparation method and application thereof |
| CN112961796A (en) * | 2021-01-29 | 2021-06-15 | 湖北华扬科技发展有限公司 | Method for producing clostridium butyricum by fermenting enterococcus faecium fermentation waste liquid |
| CN113403236A (en) * | 2021-07-26 | 2021-09-17 | 甘肃省科学院生物研究所 | Pseudomonas compound microbial agent and application thereof in disease-resistant, yield-increasing and quality-improving of angelica sinensis |
| CN113652365A (en) * | 2021-06-24 | 2021-11-16 | 沈阳农业大学 | Microbial inoculum for degrading straws at low temperature and preparation method thereof |
| CN113980829A (en) * | 2021-06-22 | 2022-01-28 | 兰州大学 | Pseudomonas flava, culture method thereof, culture product thereof, treatment agent, and repair method |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
| CN102334589A (en) * | 2011-08-03 | 2012-02-01 | 上海创博生态工程有限公司 | Method for preparing fermented feed from waste bacillus fermentation liquor |
-
2013
- 2013-05-31 CN CN201310217328.4A patent/CN103305225B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101671644A (en) * | 2009-09-28 | 2010-03-17 | 上海亘卓生物工程有限公司 | Method for preparing culture environment purifying agent by spore fermentation waste liquid |
| CN102334589A (en) * | 2011-08-03 | 2012-02-01 | 上海创博生态工程有限公司 | Method for preparing fermented feed from waste bacillus fermentation liquor |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103664254A (en) * | 2013-12-10 | 2014-03-26 | 上海师范大学 | Preparation method of compound microbial fertilizer |
| CN103664254B (en) * | 2013-12-10 | 2015-07-22 | 上海师范大学 | Preparation method of compound microbial fertilizer |
| CN110615721A (en) * | 2018-06-20 | 2019-12-27 | 四川省农业科学院生物技术核技术研究所 | Soil biological denitrifier and preparation method and application thereof |
| CN112961796A (en) * | 2021-01-29 | 2021-06-15 | 湖北华扬科技发展有限公司 | Method for producing clostridium butyricum by fermenting enterococcus faecium fermentation waste liquid |
| CN113980829A (en) * | 2021-06-22 | 2022-01-28 | 兰州大学 | Pseudomonas flava, culture method thereof, culture product thereof, treatment agent, and repair method |
| CN113652365A (en) * | 2021-06-24 | 2021-11-16 | 沈阳农业大学 | Microbial inoculum for degrading straws at low temperature and preparation method thereof |
| CN113403236A (en) * | 2021-07-26 | 2021-09-17 | 甘肃省科学院生物研究所 | Pseudomonas compound microbial agent and application thereof in disease-resistant, yield-increasing and quality-improving of angelica sinensis |
| CN113403236B (en) * | 2021-07-26 | 2022-03-08 | 甘肃省科学院生物研究所 | Pseudomonas compound microbial agent and application thereof in disease-resistant, yield-increasing and quality-improving of angelica sinensis |
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| CN103305225B (en) | 2016-08-17 |
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