CN104046363B - A kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid - Google Patents
A kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid Download PDFInfo
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- CN104046363B CN104046363B CN201310085536.3A CN201310085536A CN104046363B CN 104046363 B CN104046363 B CN 104046363B CN 201310085536 A CN201310085536 A CN 201310085536A CN 104046363 B CN104046363 B CN 104046363B
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Abstract
The present invention provides a kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid, and by composite bacteria inoculation in the medium, Anaerobic culturel, obtains composite microbial system；By composite microbial system inoculation in the fermentation medium, anaerobic fermentation, obtains soil remediation modifying agent.Gained soil remediation modifying agent contains various active microorganism, enzyme, organic acid and antibiotics, facilitation can be played to benign micro-ecological environment in crop soil, make organic and inorganic nutrients solubilized, promote sorption enhanced of the crop to nutrient, improve soil aggregate structure, suppress the growth of harmful pathogen at the same time, decompose the residual harm of agriculture in soil to a certain extent.This method can not only achieve the purpose that emission reduction; environmental protection; and making full use of nutritional ingredient in waste liquid to be used for the production of soil remediation modifying agent, cost-effective and water resource has that easy to operation, operating cost is low, is easy to the outstanding features such as industrial applications.
Description
Technical field
The present invention relates to a kind of method for preparing soil remediation modifying agent, more particularly to one kind to utilize gemma fermented waste fluid system
The method of standby soil remediation modifying agent.
Background technology
Bacillus（Bacillaceae）, a section for bacterium, can form gemma（Endospore）Bacillus or coccus.Bag
Include bacillus, Sporolactobacillus, fusobacterium, Desulfotomaculum and Sporosarcina etc..They have the external world
The sub- resistance of noxa is strong, and distribution is wide, is present in soil, water, air and animal intestinal tract etc..Bacillus bacillus bacillus
The one of section belongs to bacterium.
Research shows that bacillus can produce a variety of digestive ferments, helps animal to digest and assimilate nutriment.Gemma bar
Bacterium has stronger protease, amylase and lipase active, while also has the enzyme of complex carbohydrates in degraded feed,
Such as pectase, dextranase, cellulase, these enzymes can destroy the cell membrane of plant feed cell, promote the battalion of cell
Foster substance release comes out, and can eliminate the anti-nutritional factors in feed, reduces the obstacle that anti-nutritional factors utilizes animal digestion.
Therefore, feeding bacillus has broad application prospects in animal-breeding.
But there is ammonia nitrogen, phosphorus, a variety of enzymes that fermentation produces, residual in the fermented waste fluid that feeding bacillus produces is produced
The material such as the fermentation medium stayed and the gemma of a small amount of resting state, waste liquid COD value is in 1500mg/L or so, it is impossible to utilizes this
A little materials, fermented abandoned liquid, which directly discharges, can cause body eutrophication and environmental pollution.But this waste liquid is used for other strains
Culture, remaining nutritional ingredient therein can be made full use of, while be prepared into new product.As CN101671644A provides one kind
The method that breeding environment cleanser is prepared using gemma fermented waste fluid, water is obtained by gemma fermented waste fluid and compound bacteria sealed fermenting
Body cleanser, this method can not only achieve the purpose that emission reduction, environmental protection, and make full use of the nutritional ingredient in waste liquid to be used for
The production of breeding environment modifying agent, cost-effective and water resource.And for example CN102334589A discloses one kind and is sent out with bacillus
The method that ferment waste liquid prepares fermented feed, this method are used for cultivating lactic acid bacteria bacterium by the use of fermentation of bacillus waste liquid as nutrient solution
Kind, be conducive to environmental protection so as to solve the problems, such as the discharging of waste liquid produced pollution.The present invention utilizes fermentation of bacillus waste liquid
The industrial water in fermented feed is substituted, water-saving purpose can be reached.
Bacillus also is used as bio-feritlizer, can be in crop rhizosphere or fixed in vivo when acting on crop or soil
Grow, and play specific fertilizer effect.At present, microbial manure improves chemical fertilizer utilization ratio in culture fertility, suppresses crops to nitre
The absorption of state nitrogen, heavy metal, pesticide, purification and rehabilitating soil, reduce corps diseases, promote agricultural crop straw and city
The decomposed utilization of rubbish, environmental protection, and improve crop products quality and food security etc. and show and can not replace
The effect in generation.It can be considered to be used to gemma fermented waste fluid improve soil, but it is effective not yet to develop one kind at present
Method.
The content of the invention
It is an object of the invention to solve the above problems, there is provided one kind prepares soil remediation improvement using gemma fermented waste fluid
The method of agent.
First purpose of the present invention is to provide a kind of method that soil remediation modifying agent is prepared using gemma fermented waste fluid,
Specifically include following steps：
Step 1, by composite bacteria inoculation in the medium, Anaerobic culturel, obtains composite microbial system；Wherein, the compound bacteria
Kind includes two or more in Rhodopseudomonas palustris, lactobacillus plantarum, streptococcus lactis and saccharomyces cerevisiae；Composite bacteria with
The weight ratio of culture medium is（0.5-10）:（90-99.5）；
Step 2, by the composite microbial system obtained in step 1 inoculation in the fermentation medium, anaerobic fermentation, obtains soil remediation
Modifying agent；Wherein, composite microbial system accounts for the 10-20wt% of the gross mass of composite microbial system and fermentation medium；The fermentation medium bag
Include substrate vectors 20-50wt%, gemma fermented waste fluid 10-15wt%, industrial culture medium 10-20wt%, soil extract 2-5wt%,
Growth factor solution 0.5-1wt% and excess water.
Preferably, anaerobic fermentation conditions are in step 1：Anaerobic fermentation 100-200h at 20-40 DEG C.
It is further preferred that anaerobic fermentation conditions are in step 1：Anaerobic fermentation 120-130h at 30-37 DEG C.
Preferably, the weight ratio of composite bacteria and culture medium is in step 1（1-2）:（98-99）.
Preferably, composite bacteria described in step 1 includes 0.5 × 109Cfu/ml Rhodopseudomonas palustris 5-7wt%, 5 ×
109Cfu/ml lactobacillus plantarums 35-40wt%, 5 × 109Cfu/ml streptococcus lactis 40-45wt% and 2.5 × 109Cfu/ml beer
Two or more in saccharomycete 20-25wt%.
Preferably, culture medium described in step 1 includes molasses 1-1.2wt%, peptone 0.4-0.6wt%, yeast extract 1-
1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, ferric sulfate 3-3.5wt%, magnesium sulfate 0.02-0.03wt%,
Growth factor solution 1-1.2wt%, soil extract 10-15wt%, the water of gemma fermented waste fluid 5-7wt% and surplus.
Preferably, the growth factor solution is to include biotin 2-2.3wt%, the aqueous solution of vitamin B12-15wt%.
It is further preferred that the growth factor solution by biotin 2-2.3wt%, vitamin B12-15wt% it is water-soluble
Liquid forms.
Preferably, the pH value of culture medium described in step 1 is 5-8, more preferably 6-7, more preferably 6.5.
Preferably, anaerobic fermentation conditions are in step 2：Anaerobic fermentation 50-120h at 20-40 DEG C.
It is further preferred that anaerobic fermentation conditions are in step 2：Anaerobic fermentation 72-90h at 30-35 DEG C.
Preferably, the fermentation containing gemma that the gemma fermented waste fluid is produced by production gemma additive for microbe feedstuff
Waste liquid centrifuges, and takes supernatant to be made.
Wherein, the fermented waste fluid centrifugal condition containing gemma is preferably 6000-7000rpm centrifugations 3-3.5h.
Preferably, substrate vectors described in step 2 in blue or green Kang, peanut shell powder, corn stalk powder and miscellaneous dregs of rice at least
It is a kind of.
Preferably, industrial culture medium described in step 2 for molasses, cornstarch, beancake powder, magnesium sulfate, potassium dihydrogen phosphate,
One or more of mixtures in dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride and sodium chloride.
Preferably, the liquid that the soil extract leaches after being soaked by sterile soil when 30-36 is small.
Preferably, the method provided by the invention using gemma fermented waste fluid preparation soil remediation modifying agent is located after further including
Manage step：The tunning drying and crushing that step 2 is obtained, sieving.
Second object of the present invention is to provide one kind and prepares soil remediation improvement using gemma fermented waste fluid according to above-mentioned
Soil remediation modifying agent prepared by the method for agent.
Third object of the present invention is to provide a kind of soil remediation modification method, and soil is prepared using gemma fermented waste fluid
Modifying agent is repaired, soil remediation improvement is carried out using the soil remediation modifying agent.
Composite microbial system is inoculated in using fermented waste fluid, soil extract, growth factor by method provided by the invention（It is micro
Element）The mixing such as solution composition culture medium in soil remediation modifying agent is prepared, it contain various active microorganism, enzyme,
Organic acid and antibiotics, can play facilitation to benign micro-ecological environment in crop soil, make organic and inorganic nutrients can
Dissolve, promote sorption enhanced of the crop to nutrient, improve soil aggregate structure, while suppress the growth of harmful pathogen, one
Determine to decompose the residual harm of agriculture in soil in degree.
The method of the present invention can not only achieve the purpose that emission reduction, environmental protection, and make full use of nutrition in waste liquid into
Point for soil remediation modifying agent production, cost-effective and water resource, have it is easy to operation, operating cost is low, is easy to
The outstanding features such as industrial applications.
Embodiment
With reference to specific embodiment, the invention will be further described, to more fully understand the present invention.
Rhodopseudomonas palustris in embodiment, Rhodopseudomonas palustris, purchased from Chinese common micro- life
Thing culture presevation administrative center, numbering CGMCC1.2349；The lactobacillus plantarum, Lactobacillus plantarum, purchase
From Chinese agriculture Microbiological Culture Collection administrative center, numbering ACCC11118；The streptococcus lactis, Streptococcus
Lactis, purchased from Sichuan Province microbial collection center, numbering SCTCC100731；The saccharomyces cerevisiae,
Saccharomyces cerevisiae, purchased from Chinese agriculture Microbiological Culture Collection administrative center, numbering ACCC20065.
Embodiment 1
The first step, composite bacteria is accessed in culture medium, 120h is cultivated under the conditions of 30 DEG C, obtains composite microbial system.
Wherein, the composite bacteria includes 0.5 × 109Cfu/ml Rhodopseudomonas palustris 5wt%, 5 × 109Cfu/ml plants
Lactobacillus 35wt%, 5 × 109Cfu/ml streptococcus lactis 40wt% and 2.5 × 109Cfu/ml saccharomyces cerevisiaes 20wt%.Composite bacteria
Weight ratio with culture medium is 1:99.
Wherein, the culture medium is by molasses 1wt%, peptone 0.4wt%, yeast extract 1wt%, calcium chloride 0.1wt%, sodium chloride
0.2wt%, ferric sulfate 3.0wt%, magnesium sulfate 0.02wt%, growth factor solution 1wt%（Including biotin 2wt%, vitamin
The aqueous solution of B12wt%）, soil extract 10wt% and surplus water composition, pH6.5.
Second step, the composite microbial system inoculation that the first step is obtained in the fermentation medium, are uniformly stirred after mixing at 33.3 DEG C
Mix anaerobic fermentation 72 under conditions of device rotating speed 60r/min it is small when.Wherein composite microbial system accounts for the total of composite microbial system and fermentation medium
The 15wt% of quality.Fermentation medium is soaked by substrate vectors 30t%, gemma fermented waste fluid 10wt%, industrial culture medium 15wt%, soil
Go out liquid 2-5wt%, growth factor solution 1wt% and excess water composition.
Wherein, the gemma fermented waste fluid, by the hair containing gemma for being produced production gemma additive for microbe feedstuff
Ferment waste liquid centrifuges 3h with 6000rpm, takes supernatant to be made.
Wherein, the liquid that the sterile soil in soil extract position is leached after being soaked when 30 is small.
Wherein, the growth factor solution, is made of the aqueous solution of biotin 2wt%, vitamin B12 wt%.
Wherein, the industrial culture medium is by molasses, cornstarch, beancake powder, magnesium sulfate, potassium dihydrogen phosphate, phosphoric acid hydrogen two
Potassium, magnesium sulfate, calcium chloride, the mixture composition of sodium chloride.
Wherein, the substrate vectors are made of the mixture of blue or green Kang, peanut shell powder, corn stalk powder.
3rd step, by gained tunning drying and crushing, crosses 50 mesh sieves, that is, obtains soil remediation modifying agent.Embodiment 2
The first step, composite bacteria is accessed in culture medium, 130h is cultivated under the conditions of 37 DEG C, obtains composite microbial system.
Wherein, the composite bacteria includes 0.5 × 109Cfu/ml Rhodopseudomonas palustris 7wt%, 5 × 109Cfu/ml plants
Lactobacillus 45wt%, 5 × 109Cfu/ml streptococcus lactis 45wt% and 2.5 × 109Two kinds in cfu/ml saccharomyces cerevisiaes 25wt%
It is or a variety of.The weight ratio of composite bacteria and culture medium is 2:98.
Wherein, the culture medium by molasses 1.2wt%, peptone 0.6wt%, yeast extract 1.2wt%, calcium chloride 0.15wt%,
Sodium chloride 0.25wt%, ferric sulfate 3.5wt%, magnesium sulfate 0.03wt%, growth factor solution 1.2wt%（Including vitamin
The aqueous solution of H2.3wt%, pangamic acid wt%）, soil extract 13wt% and surplus water composition, pH6.5.
Second step, the composite microbial system mother liquor that the first step is obtained are accessed by substrate vectors 30t%, gemma with the ratio of 15wt%
Fermented waste fluid 10wt%, industrial culture medium 15wt%, soil extract 2-5wt%, growth factor solution 1wt% excess waters are formed
In large-scale fermentation medium, uniformly after mixing when anaerobic fermentation 72 is small under conditions of 33.3 DEG C, agitator speed 60r/min.
Wherein, the gemma fermented waste fluid, is fermented useless by the gemma for being produced production gemma additive for microbe feedstuff
Liquid centrifuges 3.5h with 7000rpm, takes supernatant to be made.
Wherein, the liquid that the sterile soil in soil extract position is leached after being soaked when 36 is small.
Wherein, the growth factor solution, is made of the aqueous solution of biotin 2wt%, pangamic acid wt%.
Wherein, the industrial culture medium is by molasses, cornstarch, beancake powder, magnesium sulfate, potassium dihydrogen phosphate, phosphoric acid hydrogen two
Potassium, magnesium sulfate, calcium chloride, the mixture composition of sodium chloride.
Wherein, the substrate vectors are made of the mixture of blue or green Kang, peanut shell powder, corn stalk powder.
3rd step, by gained tunning drying and crushing, crosses 50 mesh sieves, that is, obtains soil remediation modifying agent.
The specific embodiment of the present invention is described in detail above, but it is intended only as example, it is of the invention and unlimited
It is formed on particular embodiments described above.To those skilled in the art, it is any to the equivalent modifications that carry out of the present invention and
Substitute also all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Modification, all should be contained within the scope of the invention.
Claims (9)
- A kind of 1. method that soil remediation modifying agent is prepared using gemma fermented waste fluid, it is characterised in that comprise the following steps：Step 1, by composite bacteria inoculation in the medium, Anaerobic culturel, obtains composite microbial system；Wherein, the composite bacteria bag Include Rhodopseudomonas palustris, lactobacillus plantarum, streptococcus lactis and saccharomyces cerevisiae；The weight ratio of composite bacteria and culture medium is (0.5-10):(90-99.5)；Step 2, by the composite microbial system obtained in step 1 inoculation in the fermentation medium, anaerobic fermentation, obtains soil remediation improvement Agent；Wherein, composite microbial system accounts for the 10-20wt% of the gross mass of composite microbial system and fermentation medium；The fermentation medium includes Substrate vectors 20-50wt%, gemma fermented waste fluid 10-15wt%, industrial culture medium 10-20wt%, soil extract 2- 5wt%, growth factor solution 0.5-1wt% and excess water；The growth factor solution is to include biotin 2-2.3wt%, dimension The aqueous solution of raw element B12-15wt%.
- 2. according to the method described in claim 1, it is characterized in that, anaerobic fermentation conditions are in step 1：Anaerobism at 20-40 DEG C Ferment 100-200h.
- 3. according to the method described in claim 1, it is characterized in that, anaerobic fermentation conditions are in step 2：Anaerobism at 20-40 DEG C Ferment 50-120h.
- 4. according to the method described in claim 1, it is characterized in that, composite bacteria described in step 1 includes 0.5 × 109cfu/ml Rhodopseudomonas palustris 5-7wt%, 5 × 109Cfu/ml lactobacillus plantarums 35-40wt%, 5 × 109Cfu/ml streptococcus lactis 40- 45wt% and 2.5 × 109Cfu/ml saccharomyces cerevisiaes 20-25wt%.
- 5. according to the method described in claim 1, it is characterized in that, culture medium described in step 1 include molasses 1-1.2wt%, Peptone 0.4-0.6wt%, yeast extract 1-1.2wt%, calcium chloride 0.1-0.15wt%, sodium chloride 0.2-0.25wt%, sulfuric acid Iron 3-3.5wt%, magnesium sulfate 0.02-0.03wt%, growth factor solution 1-1.2wt%, soil extract 10-15wt%, bud The water of spore fermented waste fluid 5-7wt% and surplus；The growth factor solution be include biotin 2-2.3wt%, vitamin B12- The aqueous solution of 15wt%.
- 6. preparation method according to claim 1, it is characterised in that industrial culture medium described in step 2 is molasses, corn One or more in starch, beancake powder, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, magnesium sulfate, calcium chloride and sodium chloride Mixture.
- 7. method according to claim 1 or 5, it is characterised in that the gemma fermented waste fluid is by production gemma microorganism The centrifugation of the fermented waste fluid containing gemma that feed addictive is produced, takes supernatant to be made.
- A kind of 8. soil remediation modifying agent prepared in accordance with the method for claim 1.
- 9. a kind of soil remediation modification method, including according to claim 1 the method, soil is prepared using gemma fermented waste fluid Modifying agent is repaired, soil remediation improvement is carried out using the soil remediation modifying agent.
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105062493A (en) * | 2015-07-30 | 2015-11-18 | 江西新太阳生态肥业有限公司 | Preparation method of microbial soil amendment |
| CN105331556B (en) * | 2015-11-12 | 2019-02-22 | 河南兴禾生物科技有限公司 | Compound micro-ecological preparation and its preparation method and application |
| CN105967889B (en) * | 2016-06-07 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | A kind of soil conditioner comprising glutamic acid fermentation mother liquor |
| CN106010561B (en) * | 2016-06-08 | 2019-06-21 | 内蒙古阜丰生物科技有限公司 | A kind of soil conditioner containing Threonine Fermentation waste |
| CN108184836B (en) * | 2017-11-23 | 2020-12-01 | 北京联合大学 | Combined fermentation product for accelerating degradation of residual pesticide on crops |
| CN112522155B (en) * | 2020-11-17 | 2021-09-21 | 上海圣珑环境修复材料有限公司 | Bacillus licheniformis and application thereof |
| CN112961796A (en) * | 2021-01-29 | 2021-06-15 | 湖北华扬科技发展有限公司 | Method for producing clostridium butyricum by fermenting enterococcus faecium fermentation waste liquid |
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