CN106010561B - A kind of soil conditioner containing Threonine Fermentation waste - Google Patents
A kind of soil conditioner containing Threonine Fermentation waste Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
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- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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Abstract
The invention belongs to soil conditioner fields, disclose a kind of soil conditioner containing Threonine Fermentation waste, it is prepared according to following technique: 1) collecting mycoprotein and waste water, 2) hydrolysis and neutralization, 3) biological agent A is prepared, 4) auxiliary material C prepare biological agent B, 5) is prepared, 6) prepare soil conditioner.It uses soil conditioner of the present invention that can make the crop yields such as rice, sweet potato, and improves soil property.
Description
Technical field
The invention belongs to soil conditioner fields, and in particular to a kind of soil adjusting containing Threonine Fermentation waste
Agent.
Background technique
Threonine is a kind of essential amino acid, is the third limitation of the second limiting amino acid and poultry feed of pannage
Amino acid, with the extensive use of lysine, methionine composite in mixed feed, threonine, which is increasingly becoming, influences livestock and poultry life
Long major limiting factors.Threonine is mainly used as feed addictive, and production stage is concentration, crystallization, extracts thallus egg
It is white, and the mycoprotein and mother liquid disposal difficulty that generate during it are larger, higher cost, it is difficult to obtain the relatively high pair of sexual valence
Product.
In recent years, the production of various chemical fertilizer and amount of application are continuously increased, and are played to the development of agricultural production important
Impetus.But being continuously increased with applying quantity of chemical fertilizer, application disadvantage are also gradually exposed, analysis is got up main
Show the following aspects: fertilizer efficiency utilization rate is low, causes soil hardening serious, deteriorates the physicochemical property of soil, blocks soil
Hole destroys soil aggregate structure, causes unbalance soil nutrient elements, hardened, pollution and fertility decline.For above-mentioned drawback,
One kind is sought after in cultivation of fields, energy activating soil reduces the usage amount of chemical fertilizer, reduces the pollution to environment, and to farming
The soil conditioner of object volume increase.Conventional compound fertilizer is cooperated to use with soil conditioner, the shortcomings that overcome chemical fertilizer.
Summary of the invention
In order to overcome the drawbacks of the prior art, the present invention provides a kind of, and the soil containing Threonine Fermentation waste is adjusted
Agent, the soil conditioner energy activating soil, reduces the usage amount of chemical fertilizer, has both weeding function.
For achieving the above object, the invention adopts the following technical scheme:
A kind of soil conditioner containing Threonine Fermentation waste comprising following steps:
1) collect mycoprotein and waste water: the Threonine Fermentation waste that microbial fermentation threonine is generated filters, and receives
Collect mycoprotein and waste water；
2) hydrolysis and neutralization: mycoprotein is dried, and pulverizer is pulverized into powder, and is subsequently placed in reactor tank, is added
The hydrochloric acid of 5mol/L is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
With, with remaining hydrochloric acid, the pH for controlling solution is 7.0-7.2 in ammonium hydroxide after should terminating；
3) prepare biological agent A: peanut shell being crushed to obtain peanut shell powder, then with wheat bran, rapeseed dregs and fishbone dust
It is mixed evenly to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnate 12 hours, then boil
15min is cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, temperature be 28-30 DEG C condition ferment 36 hours,
Biological agent A is made；Wherein, peanut shell powder, wheat bran, rapeseed dregs and the mass ratio of fishbone dust are 20-30:15-20:8-12:
5-7；
The microbial inoculum A is prepared according to following technique: by arthrobacterium, bacillus megaterium, azotobacter chroococcum, mostly viscous bud
It is 1 × 10 that spore bacillus, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:2:2:1 volume ratio mixing to get；
4) it prepares biological agent B: peanut shell powder being broken into peanut shell powder, is added in the solution of step 2, is stirred evenly,
12 hours are stood, dregs of beans, vinasse, conch meal and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with 5
DEG C/heating rate of min is heated to 100 DEG C, then keep the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculum concentration
It is inoculated with microbial inoculum B, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains biological agent B；Wherein, flower
The mass ratio for giving birth to shell powder, the solution of step 2, dregs of beans, vinasse, conch meal and glucose is 17-22:120-150:12-15:
7-10:4-5:2-3；
The microbial inoculum B is prepared according to following technique: it is 1 that trichoderma aureoviride, candidiasis, which are cultivated respectively to concentration,
×107The bacterium solution of a/ml, then according to 1:1 volume ratio mixing to get；
5) it prepares auxiliary material C: potassium humate, peat soil and attapulgite is mixed according to the mass ratio of 2:2:1
It is even, it then grinds, crosses 200 meshes to get auxiliary material C；
6) soil conditioner is prepared: the matter by biological agent A, biological agent B and auxiliary material C according to 12-20:7-9:3-4
Amount than mixing, stir evenly, be then freeze-dried to get.
Preferably, the arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, described
Azotobacter chroococcum is ATCC No.4412, and the bacillus polymyxa is CGMCC No.1325, and the trichoderma aureoviride is ACCC
No.32248；The candidiasis is ATCC No.22023.
Above-mentioned bacterial strains can be obtained from collection or the purchase of other commercial channel.
The beneficial effect that the present invention obtains includes the following aspects:
Threonine Fermentation waste is applied all to avoid in soil conditioner preparation and steam waste liquid by the present invention
Hair is concentrated and subtracts dirty processing, reduces energy waste, improves utilization rate, and be prepared for soil conditioner, kill two birds with one stone；
The discarded mycoprotein of direct hydrolysis of the present invention provides ammonium chloride and amino acid nitrogen source abundant, both as fermentation raw material
Can be used as microbial fermentation nutriment, also can be used as the fertilizer efficiency part in soil conditioner come using；Bacterial strain compatibility is reasonable, using not
Avoid part bacterial strain cannot be in waste water so that different microbial inoculums are in optimal fermentation condition with microbial inoculum different fermentations processing mode
It survives in the culture solution of preparation；The present invention is made by the agricultural waste material of effective use naturally occurring in conjunction with microbial technique
It must expect low in cost, adjusting soil granular, maintenance element balance, the molten potassium of fixed nitrogen；Composite mineral matter can improve soil, and to soil
A variety of middle and trace elements are discharged in earth, are increase soil fertility, soil hardening is improved, moreover it is possible to be used as microbe carrier, at one stroke
Two；Microbial inoculum of the present invention is by screening and reasonable compatibility, so that regulator has fixed nitrogen water conservation, increasing crop yield is increased income, also simultaneous
Standby certain weeding function, it is environment friendly and pollution-free.
Specific embodiment
In order to be easy to understand the technical means, the creative features, the aims and the efficiencies achieved by the present invention, tie below
Specific embodiment is closed, the present invention is further explained.
Embodiment 1
A kind of soil conditioner containing Threonine Fermentation waste, is prepared according to following technique:
1) collect mycoprotein and waste water: the Threonine Fermentation waste that microbial fermentation threonine is generated filters, and receives
Collect mycoprotein and waste water；
2) hydrolysis and neutralization: mycoprotein is dried, and pulverizer is pulverized into powder, and is subsequently placed in reactor tank, is added
The hydrochloric acid of 5mol/L is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
With, with remaining hydrochloric acid, the pH for controlling solution is 7.0-7.2 in ammonium hydroxide after should terminating；
3) prepare biological agent A: peanut shell being crushed to obtain peanut shell powder, then with wheat bran, rapeseed dregs and fishbone dust
It is mixed evenly to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnate 12 hours, then boil
15min is cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, temperature be 28-30 DEG C condition ferment 36 hours,
Biological agent A is made；Wherein, peanut shell powder, wheat bran, rapeseed dregs and the mass ratio of fishbone dust are 20:15:8:5；
The microbial inoculum A is prepared according to following technique: by arthrobacterium, bacillus megaterium, azotobacter chroococcum, mostly viscous bud
It is 1 × 10 that spore bacillus, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:2:2:1 volume ratio mixing to get；
4) it prepares biological agent B: peanut shell powder being broken into peanut shell powder, is added in the solution of step 2, is stirred evenly,
12 hours are stood, dregs of beans, vinasse, conch meal and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with 5
DEG C/heating rate of min is heated to 100 DEG C, then keep the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculum concentration
It is inoculated with microbial inoculum B, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains biological agent B；Wherein, flower
The mass ratio for giving birth to shell powder, the solution of step 2, dregs of beans, vinasse, conch meal and glucose is 17:120:12:7:4:2；
The microbial inoculum B is prepared according to following technique: it is 1 that trichoderma aureoviride, candidiasis, which are cultivated respectively to concentration,
×107The bacterium solution of a/ml, then according to 1:1 volume ratio mixing to get；
5) it prepares auxiliary material C: potassium humate, peat soil and attapulgite is mixed according to the mass ratio of 2:2:1
It is even, it then grinds, crosses 200 meshes to get auxiliary material C；
6) it prepares soil conditioner: biological agent A, biological agent B and auxiliary material C is mixed according to the mass ratio of 12:7:3
Close, stir evenly, be then freeze-dried to get.
Wherein, the arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the circle
Brown nitrogen-fixing bacteria are ATCC No.4412, and the bacillus polymyxa is CGMCC No.1325, and the trichoderma aureoviride is ACCC
No.32248；The candidiasis is ATCC No.22023.
Embodiment 2
A kind of soil conditioner containing Threonine Fermentation waste, is prepared according to following technique:
1) collect mycoprotein and waste water: the Threonine Fermentation waste that microbial fermentation threonine is generated filters, and receives
Collect mycoprotein and waste water；
2) hydrolysis and neutralization: mycoprotein is dried, and pulverizer is pulverized into powder, and is subsequently placed in reactor tank, is added
The hydrochloric acid of 5mol/L is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
With, with remaining hydrochloric acid, the pH for controlling solution is 7.0-7.2 in ammonium hydroxide after should terminating；
3) prepare biological agent A: peanut shell being crushed to obtain peanut shell powder, then with wheat bran, rapeseed dregs and fishbone dust
It is mixed evenly to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, impregnate 12 hours, then boil
15min is cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, temperature be 28-30 DEG C condition ferment 36 hours,
Biological agent A is made；Wherein, peanut shell powder, wheat bran, rapeseed dregs and the mass ratio of fishbone dust are 30:20:12:7；
The microbial inoculum A is prepared according to following technique: by arthrobacterium, bacillus megaterium, azotobacter chroococcum, mostly viscous bud
It is 1 × 10 that spore bacillus, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:2:2:1 volume ratio mixing to get；
4) it prepares biological agent B: peanut shell powder being broken into peanut shell powder, is added in the solution of step 2, is stirred evenly,
12 hours are stood, dregs of beans, vinasse, conch meal and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with 5
DEG C/heating rate of min is heated to 100 DEG C, then keep the temperature 30min, cooled to room temperature, according to 10%(v/v) inoculum concentration
It is inoculated with microbial inoculum B, control is fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtains biological agent B；Wherein, flower
The mass ratio for giving birth to shell powder, the solution of step 2, dregs of beans, vinasse, conch meal and glucose is 22:150:15:10:5:3；
The microbial inoculum B is prepared according to following technique: it is 1 that trichoderma aureoviride, candidiasis, which are cultivated respectively to concentration,
×107The bacterium solution of a/ml, then according to 1:1 volume ratio mixing to get；
5) it prepares auxiliary material C: potassium humate, peat soil and attapulgite is mixed according to the mass ratio of 2:2:1
It is even, it then grinds, crosses 200 meshes to get auxiliary material C；
6) it prepares soil conditioner: biological agent A, biological agent B and auxiliary material C is mixed according to the mass ratio of 20:9:4
Close, stir evenly, be then freeze-dried to get.
Wherein, the arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the circle
Brown nitrogen-fixing bacteria are ATCC No.4412, and the bacillus polymyxa is CGMCC No.1325, and the trichoderma aureoviride is ACCC
No.32248；The candidiasis is ATCC No.22023.
Embodiment 3
The field test effect of soil conditioner prepared by the embodiment of the present invention 2: by taking rice as an example.
Control group: Common compound fertilizer (N15P15K17)；
Test group: soil conditioner prepared by Common compound fertilizer (N15P15K17)+embodiment 2.
Experimental method: experimental plot rice cultivation, the equal 10 mu of ground of the experimental plot area that two groups of fertilizer are handled respectively are selected.
Control group: Common compound fertilizer usage amount is 60kg, and test group: Common compound fertilizer usage amount is 50kg+10kg implementation
Soil conditioner prepared by example 2；Planting conditions are identical, while harvesting rice, measurement rice per mu yield, rice grain number per spike with
And mass of 1000 kernel.Experimental result: referring to table 1
Table 1
| Group | Grain number per spike (grain/fringe) | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Control group | 64.9 | 28.46 | 479.5 |
| Test group | 68.8 | 32.18 | 528.3 |
Conclusion: by table 1, it was found that, test group grain number per spike, mass of 1000 kernel and per mu yield are apparently higher than control group, have
Statistical significance.
Second year in rice stubble plants sweet potato, and fertilizer efficiency experiment is as follows: control group uses compound fertilizer (N16-P16-K16)
60kg/ mus, experimental group uses 10g/ mus of soil conditioner of compound fertilizer (N16-P16-K16) 50kg/ mus+embodiment 2.Soil property and
Planting conditions are identical, and area is 10 mu.Experimental group per mu yield is 2734Kg, and control group per mu yield is 2296Kg, per mu
20% can be increased production.
Herbicidal effect: rice test group compared with the control group, fertilising 1 month after: the weeds such as barnyard grass, herba digitariae, iron amaranth grass
Quantity significantly reduces, wherein barnyard grass and herba digitariae reduce 60%, and iron amaranth grass reduces 65%；Herbicidal effect is good, can replace or subtract
The use of few herbicide, and no pollution to the environment.
The detection of soil and fertilizer: compared with the control group, the content of available phosphorus improves 25.3% to rice test group, available potassium
Content improve 33.6%.
The contents of the present invention are not limited to cited by embodiment, and those of ordinary skill in the art are by reading description of the invention
And to any equivalent transformation that technical solution of the present invention is taken, all are covered by the claims of the invention.
Claims (2)
1. a kind of soil conditioner containing Threonine Fermentation waste, which is characterized in that the soil conditioner is according to as follows
Technique is prepared:
1) collect mycoprotein and waste water: the Threonine Fermentation waste that microbial fermentation threonine is generated filters, and collects bacterium
Body protein and waste water；
2) hydrolysis and neutralization: mycoprotein is dried, and pulverizer is pulverized into powder, and is subsequently placed in reactor tank, is added
The hydrochloric acid of 5mol/L is subject to and does not cross raw material, and stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, instead
With, with remaining hydrochloric acid, the pH for controlling solution is 7.0-7.2 in ammonium hydroxide after should terminating；
3) it prepares biological agent A: peanut shell being crushed to obtain peanut shell powder, is then mixed with wheat bran, rapeseed dregs and fishbone dust
It is uniformly mixing to obtain mixture, the waste water for accounting for the step 1) of twice of mass of mixture is added, is impregnated 12 hours, then boil 15min,
It is cooled to room temperature, the inoculum concentration kind microbial inoculum A for being 10% according to V/V, ferments 36 hours in the condition that temperature is 28-30 DEG C, life is made
Object preparation A；Wherein, peanut shell powder, wheat bran, rapeseed dregs and the mass ratio of fishbone dust are 20-30:15-20:8-12:5-7；
The microbial inoculum A is prepared according to following technique: by arthrobacterium, bacillus megaterium, azotobacter chroococcum, mostly viscous gemma bar
It is 1 × 10 that bacterium, which is cultivated respectively to concentration,8The bacterium solution of a/ml, then according to 3:2:2:1 volume ratio mixing to get；
4) it prepares biological agent B: peanut shell powder being broken into peanut shell powder, is added in the solution of step 2, is stirred evenly, stand
12 hours, dregs of beans, vinasse, conch meal and glucose are then successively added, 500 revs/min are stirred 3 minutes, then with 5 DEG C/min
Heating rate be heated to 100 DEG C, then keep the temperature 30min, cooled to room temperature is inoculated with bacterium according to the inoculum concentration that V/V is 10%
Agent B, control are fermented 36 hours in pH6.8-7.2, the condition that temperature is 28-30 DEG C, obtain biological agent B；Wherein, peanut shell
Powder, the solution of step 2, dregs of beans, vinasse, conch meal and glucose mass ratio be 17-22:120-150:12-15:7-10:
4-5:2-3；
The microbial inoculum B is prepared according to following technique: it is 1 × 10 that trichoderma aureoviride, candidiasis, which are cultivated respectively to concentration,7
The bacterium solution of a/ml, then according to 1:1 volume ratio mixing to get；
5) it prepares auxiliary material C: potassium humate, peat soil and attapulgite being mixed evenly according to the mass ratio of 2:2:1, so
After grind, cross 200 meshes to get auxiliary material C；
6) soil conditioner is prepared: the mass ratio by biological agent A, biological agent B and auxiliary material C according to 12-20:7-9:3-4
Mixing, stir evenly, be then freeze-dried to get；
The arthrobacterium is CGMCC No.7779, and the bacillus megaterium is CGMCC No.3770, the azotobacter chroococcum
For ATCC No.4412, the bacillus polymyxa is CGMCC No.1325, and the trichoderma aureoviride is ACCC No.32248；Institute
Stating candidiasis is ATCC No.22023.
2. application of the soil conditioner described in claim 1 in crop planting.
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