CN104817376B - The biological organic fertilizer prepared using lysine waste mother liquor - Google Patents
The biological organic fertilizer prepared using lysine waste mother liquor Download PDFInfo
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Abstract
The invention belongs to biofermentation industry Wastewater treating field, discloses the biological organic fertilizer prepared using lysine waste mother liquor, and the biological organic fertilizer effectively utilizes fermented abandoned mother liquor and biological organic matter, employs ammonification and granulating technique.Biological organic fertilizer overall process of the present invention produces without discarded object, environment friendly and pollution-free；The present invention is also prepared for high purity protein and feed while fertilizer is prepared.
Description
Technical field
The invention belongs to biofermentation industry Wastewater treating field, and in particular to be prepared using lysine waste mother liquor
Biological organic fertilizer.
Background technology
Lysine is one of essential amino acid, can promote human development, enhancing immunologic function, and be improved maincenter god
Effect through function of organization.Lysine is alkaline essential amino acid.Because the lysine content in cereal foods is very low, and adding
Easily it is destroyed and lacks during work, therefore referred to as the first limiting amino acids.Lysine be human body need a kind of amino acid, one
The indispensable nutriment of kind.It is known that protein is the Main Ingredients and Appearance for forming human body cell, the protein in food enters
Enter by digesting first breaks down into amino acids after human body, then human body synthesizes new human body protein again using these amino acid again,
Such as immune antiboidy, digestive ferment, plasma protein, growth hormone are all the human body proteins after synthesis.In each of synthetic protein
In kind amino acid, 1B is most important one kind, has lacked it, and other amino acid are just restricted or are not used, section
Scholar it be referred to as the essential amino acid of human body first.Scientist also found that 1B is the important substance suppression for controlling growth in humans
Most important and most required composition in long element (Somatotation, ss), to the nervous centralis and peripheral nervous system of people
System all plays an important role.Human body itself can not synthesize 1B, it is necessary to lysine is drawn from food and is to aid in other battalion
Support material only could improve food by the key substance that human body fully absorbs and utilizes, human body supplemented with enough 1Bs
The absorption and utilization of protein, reach balanced nutritious, enhancing development.At present, producing the main method of lysine includes two
Walk fermentation method, direct fermentation, enzyme process etc..But the cost of fermentation medium is higher at present, many enterprises for producing amino acid
Loss is faced, how to reduce fermentation costs is that enterprise needs to solve the problems, such as.
At present, after fermentation byproduct mycoprotein is by flocculation sediment plate-frame filtering, directly as cheap forage protein pin
Sell, added value of product is relatively low；Waste water is expelled directly out by simple process；But the above method does not enter various waste materials respectively
Row processing, does not utilize added value to greatest extent.Fermented abandoned mother liquor how is effectively utilized, improves industry to greatest extent
Added value, it is the direction that enterprise is pursued always.
The development of China's fertilizer and application history, it is from farm manure to the use of inorganic fertilizer being Main Stage, because farm manure is dirty
Dye source is more, and freight volume is big, operationally wastes time and energy and effect is not particularly evident；And inorganic fertilizer composition is single, utilization rate
It is relatively low, and often cause soil hardening and with soil erosion polluted source, influence ecological environment.At present, market is adopted more
Inorganic composite fertilizer with Nitrogen-and Phosphorus-containing potassium etc., the fertilizer efficiency of the composite fertilizer is comprehensive, but not lasting there is also fertilizer efficiency, and easily loss etc. lacks
Fall into；Importantly, the price of composite fertilizer is higher at present, larger burden is brought to peasant, how to reduce fertilizer cost, improved
Farmers' income is the agricultural modernization technical issues that need to address.
The content of the invention
The defects of in order to overcome prior art, the invention provides the biological organic prepared using lysine waste mother liquor
Fertilizer, the biological organic fertilizer are effectively utilized waste mother liquor composition, and overall process produces without discarded object, environment friendly and pollution-free；Prepare fertilizer
While, also it is prepared for high purity protein and feed.
For achieving the above object, the present invention uses following technical scheme：
The biological organic fertilizer prepared using lysine waste mother liquor, the preparation method of the biological organic fertilizer include following step
Suddenly：
Step 1）Lysine fermentation liquor collects filtrate A and solid fiber material by the filter that aperture is 1mm；Filtrate A
Through micro-filtrate membrane filtration, trapped substance and liquor B are collected respectively；Then liquor B is continued into ultrafiltration membrance filter, collect concentrate and
Liquor C；Liquor C is concentrated into a quarter of stoste volume by evaporator, then crystallizes, threonine crystal is finally collected by centrifugation
And waste liquid；Merge above-mentioned trapped substance, concentrate and waste liquid, obtain waste mother liquor；
Step 2）Waste mother liquor is heated, temperature is reached 100 DEG C, is incubated 10min, the phage solution after being denatured,
Room temperature is subsequently cooled to, the phage solution after denaturation is pumped into disk centrifugal separator, 12min is centrifuged with 4000r/min rotating speed
Afterwards, lower floor's thalline and supernatant liquid are collected；Then thalline is placed in 40 DEG C of vacuum drying chambers and dried to constant weight, subsequent ultraviolet shines
Penetrate 5min；Then thalline is added in stirred reactor, and adds the hydrochloric acid solution of the 1M equivalent to thalline double weight,
100 turns/min stirring reaction 15min, cell membrane is then removed with 3000r/min rotating speed centrifugation 5min with disc separator, received
Collect supernatant, be condensed into paste, finally by slurry-spraying pelletizing fluidized bed drying, obtain albumen；
Step 3）By step 1）Gained solid fiber material, step 2）Gained supernatant liquid and step 2）Gained cell membrane
Merge, natural subsidence separation of solid and liquid, obtain supernatant and sediment；By sediment, sorghum flour, rice chaff, egg shell powder and wheat bran
According to 5：2：1：1：1 mass ratio mixing, adds and accounts for the ammoniacal liquor of mixture quality 3%, and addition pure water adjusts moisture content to 50%,
It is well mixed, it is placed in ammonification 72 hours under closed environment；The pH for adjusting the mixture after ammonification is 5.0, and addition accounts for compounding substances
2% cellulase and 1% zytase of amount, it is well mixed, digests 20h；100 DEG C of enzyme deactivation 5min, last 30 DEG C of ventilations are dry
Dry, crushing obtains ox feed；
Step 4）Toward step 3）Humic acid potassium, diatomite, zeolite powder, dregs of beans and turf are added in gained supernatant, is stirred
Mix uniformly, stand 12 hours, then add ammoniacal liquor, stir, be placed in ammonification 72 hours under closed environment, obtain major ingredient；Will
Major ingredient, MAP, poly-aspartate, zinc sulfate are according to 200：10：1：1 mass ratio is well mixed, and is added to twin-screw and is squeezed
Go out in comminutor, be granulated packaging and Chemical Mixed Fertilizer is made.
Preferably,
The step 1）In, microfiltration membranes are inorganic ceramic membrane, and molecular cut off 3000Da, micro-filtration temperature is 36 DEG C；It is super
Membrane retention molecular weight is 300Da, and ultrafiltrate temperature is 36 DEG C.
The intensity of the ultraviolet is 2000uW/cm2。
The enzyme activity of the cellulase and zytase is respectively 5000U/g and 10000U/g.
The sorghum flour and egg shell powder are crushed by sorghum and egg shell be made respectively.
The step 4）In, step 3）Gained supernatant, humic acid potassium, diatomite, zeolite powder, dregs of beans, turf and ammonia
The mass ratio of water is 100：30：10：8：6：6：5.
Beneficial effect of the present invention mainly includes：
Because lysine waste mother liquor contains substantial amounts of fermentation thalli, albumen, sugar and fiber substance, the present invention exists
While producing lysine, make use of waste mother liquor, eliminate mother liquid disposal process, reduce production cost, realize change give up into
Treasured, the utilization rate of resource is greatly improved；The present invention is prepared for high purity protein, feed and biological organic fertilizer simultaneously, has
Effect make use of waste mother liquor composition, and overall process produces without discarded object, environment friendly and pollution-free；The present invention is using classification processing mode, system
The standby high purity protein added value of industry is high, and the feed culture efficiency of preparation is good, and the Chemical Mixed Fertilizer fertilizer efficiency of preparation is lasting, and cost is low
It is honest and clean, suitable for various crop；The present invention combines high speed centrifugation, ultraviolet irradiation and sour water solution multiple technologies, substantially increases bacterium
The cleavage rate of body, improves protein yield；Feed of the present invention uses ammonification and enzyme treatment technique, be effectively utilized fermentation waste and
Agricultural residue so that feed nutrient is more comprehensively；The present invention prepares fertilizer using waste water, avoids waste water resource, and
And provide trace element and aminoacid ingredient so that fertilizer nutrient more comprehensive and lasting；Present invention, avoiding by wastewater application
In feed, the security to feed brings hidden danger.
Embodiment
In order that those skilled in the art more fully understand the technical scheme in the application, have below in conjunction with the application
Body embodiment, the present invention is more clearly and completely described, it is clear that described embodiment is only the application one
Divide embodiment, rather than whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art are not making
The every other embodiment obtained under the premise of creative work, should all belong to the scope of protection of the invention.
Embodiment 1
The biological organic fertilizer prepared using lysine waste mother liquor, its preparation method are comprised the following steps：
1）Lysine fermentation liquor collects filtrate A and solid fiber material by the filter that aperture is 1mm；Filtrate A is through micro-
Membrane filtration, trapped substance and liquor B are collected respectively；Then liquor B is continued into ultrafiltration membrance filter, collects concentrate and filtrate
C；Liquor C is concentrated into a quarter of stoste volume by evaporator, then crystallizes, and threonine crystal is finally collected by centrifugation and gives up
Liquid；Merge above-mentioned trapped substance, concentrate and waste liquid, obtain waste mother liquor；Wherein, microfiltration membranes are inorganic ceramic membrane, retain molecule
It is 36 DEG C to measure as 3000 Da, micro-filtration temperature；Ultrafiltration retaining molecular weight is 300 Da, and ultrafiltrate temperature is 36 DEG C；
2）Waste mother liquor is heated, temperature is reached 100 DEG C, is incubated 10min, the phage solution after being denatured, then
Room temperature is cooled to, the phage solution after denaturation is pumped into disk centrifugal separator, after 4000r/min rotating speed centrifugation 12min, is received
Collect lower floor's thalline and supernatant liquid；Then thalline is placed in 40 DEG C of vacuum drying chambers and dried to constant weight, then ultraviolet irradiates
5min, thalline thickness are 10cm, uitraviolet intensity 2000uW/cm2；Then thalline is added in stirred reactor, and added
Enter the 1M of double weight hydrochloric acid solution, 100 turns/min stirring reaction 15min, then with disc separator with 3000r/min's
Rotating speed centrifugation 5min removes cell membrane, collects supernatant, is condensed into paste, finally by slurry-spraying pelletizing fluidized bed drying, obtains
To albumen；
3）By step 1）Gained solid fiber material, step 2）Gained supernatant liquid and step 2）Gained cell membrane closes
And natural subsidence separation of solid and liquid, obtain supernatant and sediment；Sediment, sorghum flour, rice chaff, egg shell powder and wheat bran are pressed
According to 5：2：1：1：1 mass ratio mixing, adds the ammoniacal liquor for accounting for mixture quality 3%, and addition pure water adjusts moisture content to 50% matter
Fraction is measured, is well mixed, is placed in ammonification 72 hours under closed environment；The pH for adjusting the mixture after ammonification is 5.0, and addition accounts for mixed
2% cellulase and 1% zytase of compound quality, it is well mixed, digests 20h；100 DEG C of enzyme deactivation 5min, last 30 DEG C
Aeration-drying, crushing obtain ox feed；The enzyme activity of the cellulase and zytase is respectively 5000U/g and 10000U/g；
The sorghum flour and egg shell powder are crushed by sorghum and egg shell be made respectively；
4）Toward step 3）Humic acid potassium, diatomite, zeolite powder, dregs of beans and turf are added in gained supernatant, stirring is equal
It is even, 12 hours are stood, ammoniacal liquor is then added, stirs, be placed in ammonification 72 hours under closed environment, obtain major ingredient；By major ingredient,
MAP, poly-aspartate, zinc sulfate are according to 200：10：1：1 mass ratio is well mixed, and is added to double-screw extruding pelletizing
In machine, it is granulated packaging and Chemical Mixed Fertilizer is made；Wherein, step 3）Gained supernatant, humic acid potassium, diatomite, zeolite powder, dregs of beans, grass
The mass ratio of charcoal and ammoniacal liquor is 100：30：10：8：6：6：5.
Embodiment 2
The albumen that the present invention prepares embodiment 1, purity can reach more than 90%, substitute in commercial standard YPD culture mediums
Dusty yeast, remaining components unchanged, saccharomyces cerevisiae and Pichia pastoris is cultivated under the same conditions, by the growing state for comparing cell
（OD600）Evaluate the culture effect of product.Specifically it is shown in Table 1：
Table 1
Conclusion：Utilize turbidimetry for Determination OD600The growing state of cell is characterized, shows that with this product commercially available ferment can be substituted
Female powder product, culture effect more preferably, can bring huge economic benefit to enterprise.
Embodiment 3
The influence that feed prepared by embodiment 1 grows for calf
It is grouped in control group and test group, every group 50, male and female half and half, is surveyed in 180 ages in days from the nascent calf of health
Determine the body weight of ox.Feed colostrum within 0-10 days after calf birth, the nursing of basal diet and feed is carried out after the 10th day.Count respectively
Average adding weight, 120~180 days average adding weights, the 180th day bust and the 180th day body were high by four at 30~90 days for calf
Item index.Control group：Basal diet：Plain edition feed=8：1；
Test group：Basal diet：Feed=8 of embodiment 1：1.It the results are shown in Table 2.
The growth indexes measure of the calf of table 2
| Group | Average daily gain in 30-90 days（Kg/ heads） | Average daily gain in 120-180 days（Kg/ heads） | 180th day bust（cm） | 180th day body height（cm） |
| Control group | 0.51 | 0.53 | 115.9 | 93.5 |
| Test group | 0.57 | 0.60 | 121.3 | 98.7 |
Conclusion：The calf speed of growth of test group is better than control group, and more obvious than having for control group on bust and body height
Advantage, illustrate eat the present invention feed after, beneficial to calf grow.
Embodiment 4
The field test effect of biological organic fertilizer prepared by the embodiment of the present invention 1：By taking wheat as an example.
Control group：Common compound fertilizer：N-P-K content is 15: 15: 15；
Test group：Biological organic fertilizer prepared by embodiment 1.
Experimental method：Experimental plot Winter Wheat Planted is selected, the equal 10 mu of ground of the experimental plot area that two groups of fertilizer are handled respectively, every group
The usage amount of experimental plot fertilizer is 40kg, and other planting conditions are identical；Harvest wheat simultaneously, measure wheat per mu yield with
And rate of growth；Detect wheat grains per spike, mass of 1000 kernel simultaneously.Experimental result：With reference to table 3.
Table 3
| Group | Grain number per spike | Mass of 1000 kernel（g） | Per mu yield (kg) |
| Control group | 28.9 | 45.3 | 365 |
| Test group | 31.2 | 46.9 | 387 |
Conclusion：By table 3 it was found that, test group wheat grains per spike, mass of 1000 kernel and per mu yield apparently higher than control group,
Possesses statistical significance.
Although above detailed explanation is made to this case with generality explanation and embodiment, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
The modification or improvement made without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (4)
1. the biological organic fertilizer prepared using lysine waste mother liquor, it is characterised in that the preparation method of the biological organic fertilizer
Comprise the following steps：
Step 1）Lysine fermentation liquor collects filtrate A and solid fiber material by the filter that aperture is 1mm；Filtrate A is through micro-
Membrane filtration, trapped substance and liquor B are collected respectively；Then liquor B is continued into ultrafiltration membrance filter, collects concentrate and filtrate
C；Liquor C is concentrated into a quarter of stoste volume by evaporator, then crystallizes, and lysine crystal is finally collected by centrifugation and gives up
Liquid；Merge above-mentioned trapped substance, concentrate and waste liquid, obtain waste mother liquor；
Step 2）Waste mother liquor is heated, temperature is reached 100 DEG C, is incubated 10min, the phage solution after being denatured, then
Room temperature is cooled to, the phage solution after denaturation is pumped into disk centrifugal separator, after 4000r/min rotating speed centrifugation 12min, is received
Collect lower floor's thalline and supernatant liquid；Then thalline is placed in 40 DEG C of vacuum drying chambers and dried to constant weight, subsequent ultraviolet irradiation
5min；Then thalline is added in stirred reactor, and adds the hydrochloric acid solution of the 1M equivalent to thalline double weight, 100
Turn/min stirring reaction 15min, cell membrane is then removed with 3000r/min rotating speed centrifugation 5min with disc separator, collected
Supernatant, paste is condensed into, finally by slurry-spraying pelletizing fluidized bed drying, obtains albumen；
Step 3）By step 1）Gained solid fiber material, step 2）Gained supernatant liquid and step 2）Gained cell membrane closes
And natural subsidence separation of solid and liquid, obtain supernatant and sediment；Sediment, sorghum flour, rice chaff, egg shell powder and wheat bran are pressed
According to 5：2：1：1：1 mass ratio mixing, adds the ammoniacal liquor for accounting for mixture quality 3%, and addition pure water adjusts moisture content to 50%, mixed
Close uniformly, be placed in ammonification 72 hours under closed environment；The pH for adjusting the mixture after ammonification is 5.0, and addition accounts for mixture quality
2% cellulase and 1% zytase, be well mixed, digest 20h；100 DEG C of enzyme deactivation 5min, last 30 DEG C of aeration-drying,
Crushing obtains ox feed；
Step 4）Toward step 3）Humic acid potassium, diatomite, zeolite powder, dregs of beans and turf are added in gained supernatant, stirring is equal
It is even, 12 hours are stood, ammoniacal liquor is then added, stirs, the supernatant, humic acid potassium, diatomite, zeolite powder, dregs of beans, grass
The mass ratio of charcoal and ammoniacal liquor is 100：30：10：8：6：6：5, ammonification 72 hours under closed environment are placed in, obtain major ingredient；Will be main
Material, MAP, poly-aspartate, zinc sulfate are according to 200：10：1：1 mass ratio is well mixed, and is added to twin-screw extrusion
In comminutor, it is granulated packaging and Chemical Mixed Fertilizer is made.
2. biological organic fertilizer according to claim 1, it is characterised in that the step 1）In, microfiltration membranes are inorganic ceramic
Film, molecular cut off 3000Da, micro-filtration temperature are 36 DEG C；Ultrafiltration retaining molecular weight is 300Da, and ultrafiltrate temperature is 36 DEG C.
3. biological organic fertilizer according to claim 1, it is characterised in that the intensity of the ultraviolet is 2000uW/cm2。
4. biological organic fertilizer according to claim 1, it is characterised in that the enzyme activity of the cellulase and zytase point
Wei not 5000U/g and 10000U/g.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106045626B (en) * | 2016-06-07 | 2019-12-24 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological bacterial fertilizer by using amino acid fermentation waste liquid and corn straw |
| CN106045701B (en) * | 2016-06-07 | 2019-07-23 | 内蒙古阜丰生物科技有限公司 | The compound fertilizer prepared using Threonine Fermentation waste |
| CN106010561B (en) * | 2016-06-08 | 2019-06-21 | 内蒙古阜丰生物科技有限公司 | A kind of soil conditioner containing Threonine Fermentation waste |
| CN106187423B (en) * | 2016-06-30 | 2019-05-24 | 内蒙古阜丰生物科技有限公司 | A kind of bio-bacterial manure comprising amino acid fermentation waste |
| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN107337569A (en) * | 2017-09-07 | 2017-11-10 | 界首市金牛家庭农场 | A kind of preparation method of tomato planting organic fertilizer |
| CN112047519A (en) * | 2020-09-18 | 2020-12-08 | 安徽华恒生物科技股份有限公司 | Comprehensive utilization method of microbial fermentation waste liquid |
| CN112142519A (en) * | 2020-09-27 | 2020-12-29 | 浙华欣农(嘉兴)生物科技有限公司 | Utilize equipment of abandonment eggshell protein preparation amino acid calcium magnesium liquid fertilizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US8623110B2 (en) * | 2007-08-02 | 2014-01-07 | Archer Daniels Midland Company | Protein and isolated or purified amino acid product containing compositions and uses thereof |
| CN101538179B (en) * | 2009-04-29 | 2011-09-28 | 马健 | Method for preparing compound amino acid organic liquid fertilizer |
| CN101812009B (en) * | 2010-04-28 | 2011-12-14 | 河南巨龙生物工程股份有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
| CN102442919B (en) * | 2010-10-13 | 2013-09-04 | 中粮生物化学（安徽）股份有限公司 | Method for producing lysine product |
| CN102442920B (en) * | 2010-10-13 | 2014-10-15 | 中粮生物化学（安徽）股份有限公司 | Method for processing lysine fermentation broth |
| CN102719510B (en) * | 2012-06-26 | 2013-12-18 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN102911070A (en) * | 2012-11-13 | 2013-02-06 | 河南巨龙生物工程股份有限公司 | Technology for separating and extacting L-threonine from fermentation broth |
| CN104058844B (en) * | 2014-06-09 | 2016-04-20 | 李学春 | Threonine mother liquor is utilized to prepare the method for multicomponent chelate thing fertilizer |
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