CN107164247A - A kind of method of utilization L alanine fermentation wastes culture yeasts - Google Patents
A kind of method of utilization L alanine fermentation wastes culture yeasts Download PDFInfo
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- CN107164247A CN107164247A CN201710410683.1A CN201710410683A CN107164247A CN 107164247 A CN107164247 A CN 107164247A CN 201710410683 A CN201710410683 A CN 201710410683A CN 107164247 A CN107164247 A CN 107164247A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a kind of method of utilization L alanine fermentation wastes culture yeasts, it is related to yeast fermented and cultured technical field, thalline and high molecular weight protein in L alanine zymotic fluids is filtered out first；Then the thalline and high molecular weight protein that filter out are hydrolyzed with acid-hydrolysis method, form discarded mycoprotein hydrolyzate；Discarded mycoprotein hydrolyzate pH value is neutralized to 6.8 ~ 7.2 with alkaline reagent again, finally yeast fermentation medium is prepared with the discarded mycoprotein hydrolyzate after neutralizing.Direct hydrolysis L alanine discarded object of the present invention provides abundant amino acid nitrogen source for Yeast Cultivation, can realized the re-using of waste as microbial fermentation nutriment, reduce the cost of yeast fermentation medium as yeast fermentation raw material；Yeast well-grown in its culture medium being formulated, significantly reduces the treating capacity of fermentation wastes during fermentation method production L alanine, not only reduces environmental pollution, and greatly improve its added value.
Description
Technical field
It is particularly a kind of to utilize ALANINE fermentation wastes culture ferment the present invention relates to yeast fermented and cultured technical field
Female method.
Background technology
Biofermentation enterprise produces in substantial amounts of discarded object, the discarded object and had during the various amino acid of fermenting and producing
There are COD, SO4 2-、PO4 3-And NH3The features such as-N content is high, and containing substantial amounts of discarded thalline, wherein rich in protein, nucleic acid
Deng nutriment.Processing method cost height at present for the discarded object, difficulty are big, how to effectively utilize discarded mycoprotein
It is the problem of needing to solve.
Yeast has very big value as single cell protein, and its main component is protein, and protein almost account for
The content of yeast dry matter half, and essential amino acid content is sufficient, is especially enriched in the lysine relatively lacked in cereal；
On the other hand, also containing a large amount of vitamin B1s, vitamin B2 and niacin.Moreover, its amino acid ratio is close to the United Nations
The comparatively ideal amino acid composition value that food and agricultural organization (FAO) is recommended.Yeast is less demanding to culture medium, can using multiple protein and
Amino acid does nitrogen source.
The content of the invention
In view of the above-mentioned problems, the invention provides a kind of method of utilization ALANINE fermentation wastes culture yeasts, it is full
Sufficient modern industry automation, the demand of serialization.
A kind of method of utilization ALANINE fermentation wastes culture yeasts, filters out the bacterium in ALANINE zymotic fluid first
Body and high molecular weight protein；Then the thalline and high molecular weight protein that filter out are hydrolyzed with acid-hydrolysis method, form discarded thalline egg
White hydrolyzate；Discarded mycoprotein hydrolyzate pH value is neutralized to 6.8 ~ 7.2 with alkaline reagent again, finally with discarded after neutralization
Mycoprotein hydrolyzate prepares yeast fermentation medium.
Further, the thalline being filtrated to get and high molecular weight protein are configured to concentration for 10g/100mL ~ 30g/100mL
Discarded mycoprotein liquid, sour water solution then is carried out to it again；The sour hydrolyzed PH value condition is 0.5 ~ 2.5, and temperature conditionss are
85 ~ 115 DEG C, hydrolysis time is controlled in 24 ~ 72h.
Further, the discarded mycoprotein hydrolyzate accounts for the 5% ~ 25% of yeast fermentation medium cumulative volume.
Further, confactor is also included in the yeast fermentation medium, the confactor is dusty yeast, soybean
The combination of any one or more in peptone, beef extract-peptone, glucose, inorganic microelement；The dusty yeast concentration
Control in 5 ~ 10g/L, soy peptone concentration control in 5 ~ 10g/L, the beef extract-peptone concentration control 5 ~
10g/L, the concentration of glucose control is 1 ~ 4%, and the inorganic microelement concentration control is in 6 ~ 10mg/L.
Further, discarded mycoprotein hydrolyzate, 0.5 ~ 1% soybean egg of the yeast fermentation medium for 5 ~ 25%
White peptone, 1 ~ 4% glucose, 6 ~ 10mg/L inorganic microelement are sequentially added in water, are stirred, then at 115 ~ 121 DEG C
At a temperature of maintain 30min to carry out sterilization treatment, then be cooled to 25 ~ 30 DEG C, be formulated.
Further, the saccharomycete uses saccharomyces cerevisiae, the saccharomycete connecing in the yeast fermentation medium
Amount control is planted 3% ~ 10%, and 3 ~ 5d of culture at a temperature of 25 ~ 35 DEG C.
Direct hydrolysis ALANINE discarded object of the present invention provides abundant ammonia for Yeast Cultivation as yeast fermentation raw material
Base acid nitrogen source, the re-using of waste can be realized as microbial fermentation nutriment, reduce yeast fermentation medium into
This；Yeast well-grown in its culture medium being formulated, fermentation is useless during significantly reducing fermentation method production ALANINE
The treating capacity of gurry, not only reduces environmental pollution, and greatly improve its added value.
Embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only a part of embodiment of the invention, rather than whole embodiments.Based on the embodiment in the present invention, the common skill in this area
The every other embodiment that art personnel are obtained, belongs to the scope of protection of the invention.
Embodiment 1
200 L ALANINE zymotic fluids are filtered by ceramic membrane, ALANINE discarded object is obtained.ALANINE is discarded
Thing initial pH value is 6.98, ALANINE discarded object is adjusted into the g/100 mL waste liquids of concentration 30, with 6 mol/L hydrochloric acid
Solution adjusts pH value to 0.5, and 72 h are hydrolyzed at a temperature of 95 DEG C, mycoprotein hydrolyzate must be both discarded；Then with 6 mol/L's
Sodium hydroxide solution is by discarded mycoprotein hydrolyzate and pH value is to 7.2.
Each raw material for standby is taken according to mass ratio, wherein discarded mycoprotein hydrolyzate 15%, soy peptone 2.5%, grape
Sugar 7%, the mg/L of inorganic microelement 6, remaining is water；By discarded mycoprotein hydrolyzate, soy peptone, glucose and inorganic
Trace element is sequentially added in water, is stirred, and 30 min that then held time under the conditions of 115 DEG C of temperature are carried out at sterilizing
Reason, then it is cooled to 25 ~ 35 DEG C, obtained yeast fermentation medium.
Below by way of control group experiment, illustrate feasibility and its effect of the present invention for Yeast Cultivation.
Control group：Fermentation medium uses 3% dusty yeast, 4% soy peptone, 4% glucose and the inorganic micro members of 6 mg/L
Element, remaining is water.
The present embodiment and control group zymotechnique are in accordance with the following steps：By the saccharomyces cerevisiae in exponential phase according to
5%（Volume ratio）Fermentation medium in the inoculum concentration access control medium and the present embodiment of ratio, at a temperature of 27 ~ 30 DEG C,
200rpm fermented and cultureds 3 days, and survey its OD in 550 nm（Optical density, optical density）Value.Two groups of yeast fermented and cultureds
Yeast growth situation see the table below 1 in base.
| Fermentation time | Control group OD550 | Implementation group OD550 |
| Blank cultures | 0.7 | 1.41 |
| Culture 1 day | 10.6 | 7.6 |
| Culture 2 days | 15.1 | 14.05 |
| Culture 3 days | 18.6 | 17.5 |
Table 1
Conclusion：Saccharomycete bacterium is dense after the present embodiment and control group culture 3 days is more or less the same, and the present embodiment is lower slightly；Pass through cost core
Test, the culture medium cost that the present embodiment is formulated only accounts for 1/3rd or so of control group culture medium cost, greatlys save
Enterprise puts into, and improves enterprise's net income, while ALANINE fermentation wastes are further utilized, reduces resource wave
Take.
Finally, in addition it is also necessary to it is noted that listed above is only a specific embodiment of the invention.Obviously, the present invention is not limited
In above example, there can also be many deformations.One of ordinary skill in the art can directly lead from present disclosure
All deformations for going out or associating, are considered as protection scope of the present invention.
Claims (7)
1. a kind of method of utilization ALANINE fermentation wastes culture yeasts, it is characterised in that filter out ALANINE hair first
Thalline and high molecular weight protein in zymotic fluid；Then the thalline and high molecular weight protein that filter out are hydrolyzed with acid-hydrolysis method, formed
Discarded mycoprotein hydrolyzate；Discarded mycoprotein hydrolyzate pH value is neutralized to 6.8 ~ 7.2, in finally using with alkaline reagent again
Discarded mycoprotein hydrolyzate with after prepares yeast fermentation medium.
2. the method for utilization ALANINE fermentation wastes culture yeasts according to claim 1, it is characterised in that incited somebody to action
Filter obtained thalline and high molecular weight protein is configured to the discarded mycoprotein liquid that concentration is 10g/100mL ~ 30g/100mL, then
Sour water solution is carried out to it again；The sour hydrolyzed PH value condition is 0.5 ~ 2.5, and temperature conditionss are 85 ~ 115 DEG C, hydrolysis time control
In 24 ~ 72h.
3. the method for utilization ALANINE fermentation wastes culture yeasts according to claim 2, it is characterised in that described
Discarded mycoprotein hydrolyzate accounts for the 5% ~ 25% of yeast fermentation medium cumulative volume.
4. the method for utilization ALANINE fermentation wastes culture yeasts according to claim 3, it is characterised in that described
In yeast fermentation medium also include confactor, the confactor be dusty yeast, soy peptone, beef extract-peptone,
The combination of any one or more in glucose, inorganic microelement.
5. the method for utilization ALANINE fermentation wastes culture yeasts according to claim 4, it is characterised in that described
Dusty yeast concentration is controlled in 5 ~ 10g/L, and the soy peptone concentration control is in 5 ~ 10g/L, the beef extract-peptone concentration
Control is in 5 ~ 10g/L, and the concentration of glucose control is 1 ~ 4%, and the inorganic microelement concentration control is in 6 ~ 10mg/L.
6. the method for utilization ALANINE fermentation wastes culture yeasts according to claim 5, it is characterised in that described
Yeast fermentation medium for 5 ~ 25% discarded mycoprotein hydrolyzate, 0.5 ~ 1% soy peptone, 1 ~ 4% glucose, 6 ~
10mg/L inorganic microelement is sequentially added in water, is stirred, and then maintains 30min to carry out at a temperature of 115 ~ 121 DEG C
Sterilization treatment, then it is cooled to 25 ~ 30 DEG C, it is formulated.
7. the method for the utilization ALANINE fermentation wastes culture yeasts according to claim 1-6 any one, it is special
Levy and be, the saccharomycete uses saccharomyces cerevisiae, inoculum concentration control of the saccharomycete in the yeast fermentation medium exists
3% ~ 10%, and 3 ~ 5d of culture at a temperature of 25 ~ 35 DEG C.
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| CN201710410683.1A CN107164247A (en) | 2017-06-03 | 2017-06-03 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110846351A (en) * | 2019-12-22 | 2020-02-28 | 赵兰坤 | Threonine fermentation medium prepared by using mycoprotein as raw material |
| CN113999059A (en) * | 2021-11-15 | 2022-02-01 | 秦皇岛华恒生物工程有限公司 | Amino acid slow release fertilizer and preparation method and application thereof |
Citations (5)
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|---|---|---|---|---|
| CN101979627A (en) * | 2010-10-08 | 2011-02-23 | 天津科技大学 | Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli |
| CN102719510A (en) * | 2012-06-26 | 2012-10-10 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN102732589A (en) * | 2012-06-26 | 2012-10-17 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating threonine mother liquor |
| CN104726335A (en) * | 2015-04-13 | 2015-06-24 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for manufacturing granule proteins by aid of waste mother liquid of lysine |
| CN104817376A (en) * | 2015-04-11 | 2015-08-05 | 呼伦贝尔东北阜丰生物科技有限公司 | Bio-organic fertilizer prepared from lysine waste mother liquor |
-
2017
- 2017-06-03 CN CN201710410683.1A patent/CN107164247A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101979627A (en) * | 2010-10-08 | 2011-02-23 | 天津科技大学 | Method for preparing glutamic acid fermentation organic nitrogen additive from glutamic acid fermentation waste thalli |
| CN102719510A (en) * | 2012-06-26 | 2012-10-10 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN102732589A (en) * | 2012-06-26 | 2012-10-17 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for treating threonine mother liquor |
| CN104817376A (en) * | 2015-04-11 | 2015-08-05 | 呼伦贝尔东北阜丰生物科技有限公司 | Bio-organic fertilizer prepared from lysine waste mother liquor |
| CN104726335A (en) * | 2015-04-13 | 2015-06-24 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for manufacturing granule proteins by aid of waste mother liquid of lysine |
Non-Patent Citations (3)
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| CAZZULO JUAN JOSÉ等: "On the production of glycerol and L-alanine during the aerobic fermentation of glucose by trypanosomatids", 《FEMS MICROBIOLOGY LETTERS》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110846351A (en) * | 2019-12-22 | 2020-02-28 | 赵兰坤 | Threonine fermentation medium prepared by using mycoprotein as raw material |
| CN113999059A (en) * | 2021-11-15 | 2022-02-01 | 秦皇岛华恒生物工程有限公司 | Amino acid slow release fertilizer and preparation method and application thereof |
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Application publication date: 20170915 |