CN104726335A - Method for manufacturing granule proteins by aid of waste mother liquid of lysine - Google Patents
Method for manufacturing granule proteins by aid of waste mother liquid of lysine Download PDFInfo
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- CN104726335A CN104726335A CN201510171657.9A CN201510171657A CN104726335A CN 104726335 A CN104726335 A CN 104726335A CN 201510171657 A CN201510171657 A CN 201510171657A CN 104726335 A CN104726335 A CN 104726335A
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Abstract
The invention belongs to the field of fermentation waste liquid treatment in the biological fermentation industries, and discloses a method for manufacturing granule proteins by the aid of waste mother liquid of lysine. The method includes collecting lysine crystals and the waste mother liquid; manufacturing the granule proteins by means of centrifugal hydrolysis; manufacturing fodder and compound fertilizers by the aid of waste. The method has the advantages that the granule proteins manufactured by the aid of the method are high in purity and can replace commercially available yeast powder products, excellent culture effects can be realized, and the method can bring huge economic benefits for enterprises.
Description
Technical field
The invention belongs to biological fermentation industry Wastewater treating field, be specifically related to a kind of method utilizing Methionin waste liquor to produce granule protein.
Background technology
Methionin, as one of most important amino acid, is widely used in the industries such as medicine, food, feed.In recent years, domestic and international fodder industry and foodstuffs industry fast development, the purposes that on medicine industry, Methionin is new is constantly found, and Methionin has become good development prospect on a kind of world market, the product that domestic market is very good.After 2003, along with the numerous and confused operation of large-scale Methionin enterprise, output also starts significantly to increase year by year.Within 2003, China's lysine production reaches 7.8 ten thousand tons; 2004 17.3 ten thousand tons; 2005 33.6 ten thousand tons, and change Methionin net exporter into.Within 2006, produce 52.2 ten thousand tons, account for 60% of Gross World Product.
Methionin produce in major part be using corn steep liquor and molasses as fermentation nitrogen source, lysine fermentation liquor is by directly carrying out Methionin extraction after membrane filtration, fermentation byproduct tropina is by after flocculation sediment Plate Filtration, in product albumen and amino acid products content lower, directly sell as cheap tropina, added value of product is lower.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of method utilizing Methionin waste liquor to produce granule protein.Membrane filtration and mist projection granulating fluidized-bed combine by the method, prepare a kind of high purity granule protein.Products obtained therefrom character is: protein content >=85%, aminoacids content >=85%, moisture≤3.0%, particle diameter 0.6-1.6mm.The method has prepared feed and Chemical Mixed Fertilizer simultaneously, and effectively make use of waste mother liquor composition, whole process produces without waste, environment friendly and pollution-free.
For achieving the above object, the present invention is by the following technical solutions:
Utilize Methionin waste liquor to produce a method for granule protein, it comprises the steps:
Step 1) lysine fermentation liquor is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Methionin crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste liquor;
Step 2) waste liquor is heated, make temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, then uviolizing 5min; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of the 1M being equivalent to thalline twice quality, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant liquor, be condensed into paste, then mist projection granulating fluidized-bed is entered, wet stock and warm air are at air distribution plate overlying contact, material particles is suspended among air-flow, form fluidized state, evenly, fully mix with warm air, thus reach the object preparing granule protein.
Step 3) is by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50%, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain feed;
Step 4) adds potassium humate, diatomite and straw powder in step 3) gained supernatant liquor, stirs, and leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Mixed by major ingredient, monoammonium phosphate, poly aspartic acid and the zinc sulfate mass ratio according to 200:10:1:1, join in double-screw extruding pelletizing machine, granulation is packed and be get final product.
In described step 1), microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300 Da, and ultrafiltrate temperature is 36 DEG C.
Described ultraviolet intensity is 2000uW/cm
2;
The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
Described step 2) in, mist projection granulating fluidized-bed is made up of 1 cover mist projection granulating fluidized-bed main frame 1# bed, 1 cover dry cooling bed 2# bed, air inlet system, outlet system of air, product processing system and slip treatment system; Dry inlet temperature 150-165 DEG C of 1# bed, slurry temperature 50 DEG C, each spray gun (by 26 rod spears) flow 0.4m3/h, bed pressure reduction 8.3-8.8KPa; Dry inlet temperature≤150 of 2# bed DEG C, cooling air intake 20 DEG C, bed pressure reduction 3.5-5.0KPa.
In described step 4), the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, straw powder and ammoniacal liquor is 50:20:12:10:5
Beneficial effect of the present invention mainly comprises:
Because lysine mother liquor contains a large amount of fermentation thalli, albumen, sugar and fiber substance, the present invention, while production Methionin, make use of waste mother liquor, eliminate mother liquid disposal process, reduce production cost, achieve and turn waste into wealth, increased substantially the utilization ratio of resource; The present invention has prepared high purity granule protein, feed and Chemical Mixed Fertilizer simultaneously, and effectively make use of waste mother liquor composition, whole process produces without waste, environment friendly and pollution-free; The present invention adopts stage treatment mode, and the high purity protein added value of industry of preparation is high, and the feed culture efficiency of preparation is good, and the Chemical Mixed Fertilizer fertilizer efficiency of preparation is lasting, with low cost, is applicable to various crop; The present invention, in conjunction with high speed centrifugation, uv irradiating and acid hydrolysis multiple technologies, substantially increases the cleavage rate of thalline, improves protein yield; Feed of the present invention adopts ammonification and enzyme treatment technique, effectively make use of fermentation waste and agricultural waste, makes feed nutrient more comprehensively; The present invention adopts waste water to prepare fertilizer, avoids waste water resource, and provides trace element and aminoacid component, makes fertilizer nutrient comprehensive and lasting more.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, carry out clearly, intactly describing to the present invention, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
Utilize Methionin waste liquor to produce a method for granule protein, it comprises the steps:
1) lysine fermentation liquor (lysine content 10g/100ml) is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Methionin crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste liquor; Wherein, microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000 Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300 Da, and ultrafiltrate temperature is 36 DEG C;
2) waste liquor is heated, make temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, then uviolizing 5min, thalline thickness is 10cm, and uitraviolet intensity is 2000uW/cm
2; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of 1M, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min subsequently with disc separator, collect supernatant liquor, be condensed into paste, finally by mist projection granulating fluidised bed drying, obtain granule protein; Wherein, the mass ratio of thalline and hydrochloric acid soln is 1:2;
3) by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50% massfraction, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain feed;
The enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g;
4) in step 3) gained supernatant liquor, add potassium humate, diatomite and straw powder, stir, leave standstill 12 hours, then add ammoniacal liquor, stir, to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Major ingredient, monoammonium phosphate, poly aspartic acid and the zinc sulfate mass ratio according to 200:10:1:1 is mixed, joins in double-screw extruding pelletizing machine, the obtained Chemical Mixed Fertilizer of granulation packaging; Wherein, the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, straw powder and ammoniacal liquor is 50:20:12:10:5.
Embodiment 2
The granule protein that embodiment 1 is prepared by the present invention, purity can reach more than 85%, substitutes the yeast powder in commercial standard YPD substratum, all the other components unchanged, cultivate yeast saccharomyces cerevisiae and pichia spp under the same conditions, evaluated the culture effect of product by the growing state (OD600) comparing cell.Specifically in table 1:
Table 1
| Group | Yeast saccharomyces cerevisiae cultivates 20h | Pichia spp cultivates 20h |
| Embodiment 1 | 2.34 | 1.63 |
| Commercially available yeast powder | 1.99 | 1.51 |
Conclusion: utilize turbidimetry for Determination OD
600characterize the growing state of cell, show can substitute commercially available yeast powder product with this product, culture effect is better, can bring huge economic benefit to enterprise.
Embodiment 3
Feed culture experiment prepared by embodiment 1:
Choose the piglet 60 of 60 days, be divided into two groups, often organize 30, wherein the diet prepare with the present embodiment 1 of experimental group, control group is with leading to hog grower feed in prestige; Other rearing conditions two group is identical.Raise experimental group and control group after 9 weeks and do not have significant difference in the index such as day weight gain and feed-weight ratio, the day weight gain of control group and test group is 0.68 and 0.69kg respectively, feed-weight ratio is respectively 2.39 and 2.43, two group raising situations are suitable, feed prepared by the present invention can substitute market common feedstuffs, can greatly reduce cost.Embodiment 4
Chemical Mixed Fertilizer rice cultivation test prepared by embodiment 1:
Arrange two process experimental plots, area is ten mu, is control group and experimental group.Control group: composite fertilizer (N16-P16-K16); Experimental group: Chemical Mixed Fertilizer prepared by embodiment 1; The usage quantity often organizing experimental plot fertilizer is 60kg, and planting conditions is identical, gathers in the crops paddy rice simultaneously, measures paddy rice per mu yield and stimulation ratio; Detect paddy rice grain number per spike, thousand seed weight and unfilled gtains number simultaneously.Test-results is in table 2:
Table 2
| Group | Unfilled gtains number (grain/fringe) | Grain number per spike (grain/fringe) | Thousand seed weight (g) | Per mu yield (kg) |
| Control group | 5.1 | 64.9 | 29.7 | 473.2 |
| Experimental group | 4.9 | 65.3 | 30.1 | 481.7 |
30,000 tons of Methionin projects are produced per year for Hulunbuir Northeast Fufeng Biotechnology Co., Ltd., monthly can produce granule protein 300 tons, price 4000 yuan/ton, former tropina price 2000 yuan/ton, monthly also can increase sales revenue 600,000 yuan, for enterprise brings good economic benefit.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (6)
1. utilize Methionin waste liquor to produce a method for granule protein, it is characterized in that, described method comprises the steps:
Step 1): lysine fermentation liquor is the strainer of 1mm through aperture, collects filtrate A and solid fiber material; Filtrate A, through micro-filtrate membrane filtration, collects trapped substance and liquor B respectively; Then liquor B is proceeded ultrafiltration membrance filter, collect concentrated solution and liquor C; Liquor C is concentrated into 1/4th of stoste volume through evaporator, then crystallization, last collected by centrifugation Methionin crystal and waste liquid; Merge above-mentioned trapped substance, concentrated solution and waste liquid, obtain waste liquor;
Step 2): waste liquor is heated, makes temperature reach 100 DEG C, insulation 10min, obtain the phage solution after sex change, be then cooled to room temperature, the phage solution after sex change is pumped in disk centrifugal separator, after the centrifugal 12min of the rotating speed of 4000r/min, collect lower floor's thalline and supernatant liquid; Then thalline is placed in 40 DEG C of vacuum drying ovens to dry to constant weight, uviolizing 5min subsequently; Then thalline is added in stirred reactor, and add the hydrochloric acid soln of the 1M being equivalent to thalline twice quality, 100 turns/min stirring reaction 15min, cell walls is removed with the centrifugal 5min of the rotating speed of 3000r/min again with disc separator, collect supernatant liquor, be condensed into paste, finally by mist projection granulating fluidised bed drying, obtain granule protein;
Step 3): by step 1) gained solid fiber material, step 2) gained supernatant liquid and step 2) gained cell walls merges, and natural subsidence solid-liquid separation, obtains supernatant liquor and throw out; Throw out, rice chaff, egg shell powder and the wheat bran mass ratio according to 5:2:1:1 is mixed, adds the ammoniacal liquor accounting for mixture quality 3%, add pure water adjustment water ratio to 50%, mix, to be placed under closed environment ammonification 72 hours; The pH of the mixture after adjustment ammonification is 5.0, adds the cellulase of 2% and the zytase of 1% that account for mixture quality, mixes, enzymolysis 20h; 100 DEG C of enzyme 5min that go out, last 30 DEG C of air seasonings, pulverize and obtain feed;
Step 4): add potassium humate, diatomite and straw powder in step 3) gained supernatant liquor, stir, leaves standstill 12 hours, then adds ammoniacal liquor, stir, and to be placed under closed environment ammonification 72 hours, to obtain major ingredient; Mixed by major ingredient, monoammonium phosphate, poly aspartic acid and the zinc sulfate mass ratio according to 200:10:1:1, join in double-screw extruding pelletizing machine, granulation is packed and be get final product.
2. method according to claim 1, is characterized in that, in described step 1), microfiltration membrane is inorganic ceramic film, and molecular weight cut-off is 3000Da, and micro-filtration temperature is 36 DEG C; Ultra-filtration membrane molecular weight cut-off is 300Da, and ultrafiltrate temperature is 36 DEG C.
3. method according to claim 1 and 2, is characterized in that, described ultraviolet intensity is 2000uW/cm
2.
4. method according to claim 1, is characterized in that, described mist projection granulating fluidized-bed is made up of 1 cover mist projection granulating fluidized-bed main frame 1# bed, 1 cover dry cooling bed 2# bed, air inlet system, outlet system of air, product processing system and slip treatment system; Dry inlet temperature 150-165 DEG C of 1# bed, slurry temperature 50 DEG C, each gun traffic 0.4m3/h, bed pressure reduction 8.3-8.8KPa; Dry inlet temperature≤150 of 2# bed DEG C, cooling air intake 20 DEG C, bed pressure reduction 3.5-5.0KPa.
5. let alone the method described according to claim 1-3, it is characterized in that, the enzyme of described cellulase and zytase is lived and is respectively 5000U/g and 10000U/g.
6. let alone the method described according to claim 1-4, it is characterized in that, in described step 4), the mass ratio of step 3) gained supernatant liquor, potassium humate, diatomite, straw powder and ammoniacal liquor is 50:20:12:10:5.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN108796004A (en) * | 2018-06-20 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of technique of preparing lysine through fermentation |
| CN109321434A (en) * | 2018-12-05 | 2019-02-12 | 江苏澳创生物科技有限公司 | A kind of tryptophan feed additive production system |
| CN113896579A (en) * | 2021-09-18 | 2022-01-07 | 贵州省贵福生态肥业有限公司 | Special carbon-based microbial fertilizer deep fermentation device for tea and use method thereof |
| CN116584576A (en) * | 2023-05-25 | 2023-08-15 | 寿光玖鼎生物科技有限公司 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
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| CN101812009A (en) * | 2010-04-28 | 2010-08-25 | 河南巨龙淀粉实业有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
| CN102719510A (en) * | 2012-06-26 | 2012-10-10 | 呼伦贝尔东北阜丰生物科技有限公司 | Amino acid fermentation bacteria utilization method |
| CN103918873A (en) * | 2013-01-14 | 2014-07-16 | 上海凯鑫分离技术有限公司 | Comprehensive utilization process of glutamic acid crystal mother liquid |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101812009A (en) * | 2010-04-28 | 2010-08-25 | 河南巨龙淀粉实业有限公司 | Novel technique for extracting L-tryptophan from fermentation broth |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164247A (en) * | 2017-06-03 | 2017-09-15 | 秦皇岛华恒生物工程有限公司 | A kind of method of utilization L alanine fermentation wastes culture yeasts |
| CN108796004A (en) * | 2018-06-20 | 2018-11-13 | 齐齐哈尔龙江阜丰生物科技有限公司 | A kind of technique of preparing lysine through fermentation |
| CN109321434A (en) * | 2018-12-05 | 2019-02-12 | 江苏澳创生物科技有限公司 | A kind of tryptophan feed additive production system |
| CN113896579A (en) * | 2021-09-18 | 2022-01-07 | 贵州省贵福生态肥业有限公司 | Special carbon-based microbial fertilizer deep fermentation device for tea and use method thereof |
| CN116584576A (en) * | 2023-05-25 | 2023-08-15 | 寿光玖鼎生物科技有限公司 | Method for mixed fermentation of feed yeast protein particles by amino acid mother liquor and corn steep liquor |
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