CN108658798A - The fermentation preparation process of particle threonine - Google Patents
The fermentation preparation process of particle threonine Download PDFInfo
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- CN108658798A CN108658798A CN201810414666.XA CN201810414666A CN108658798A CN 108658798 A CN108658798 A CN 108658798A CN 201810414666 A CN201810414666 A CN 201810414666A CN 108658798 A CN108658798 A CN 108658798A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C227/00—Preparation of compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C227/38—Separation; Purification; Stabilisation; Use of additives
- C07C227/40—Separation; Purification
- C07C227/42—Crystallisation
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Abstract
The invention belongs to technical field of amino acid production, the fermentation preparation processes of particle threonine comprising following steps：Threonine fermentation liquid first passes around disk plate centrifuge centrifugation, collects supernatant, then passes through ceramic membrane filter, then filtrate is concentrated, then with sulfuric acid tune pH, is heated to 75 DEG C, then detach through decanter centrifuge, and removal precipitation collects supernatant；Then pass through ultrafiltration membrance filter, collect filtered solution, the intermittent single-action condensing crystallizing pot of filtered solution is crystallized, crystal is collected by centrifugation, then 120 DEG C of dryings are compressed slabbing, then are put into granulation tower, fluidized bed drying, again through being crushed whole grain, sieving is collected the granule of target grain size, is packed to obtain the final product.Threonine is prepared into graininess by the present invention, can be improved mobility, convenient for storage and transport, control solubility, be improved the quality and added value of threonine.
Description
Technical field
The invention belongs to amino acids production fields, and in particular to the fermentation preparation process of particle threonine.
Background technology
Threonine（Threonine is abbreviated as Thr）, scientific name 2 amino 3 hydroxybutyric acid belongs to aliphatic amino acid, micro-
Sweet tea is gained the name because structure is similar to threose, is a kind of essential amino acid for constituting people and plant animal protein, is mainly used for curing
Medicine, chemical reagent, nutrition fortifier can strengthen dairy products, have and restore human-body fatigue, the effect of enhancing development.Closely
Nian Lai, with the development of economy, market to threonine requirement sustained, stable growth, be the most fast amino acid kind of demand growth it
One, especially the dosage rapid development in chemical and biochemistry, food additives, feed addictive etc., big substituted color ammonia
Acid and as in addition to lysine, methionine development most rapidly the third-largest amino acid.L- Soviet Unions ammonia is added in mixed feed
Acid has the characteristics that as follows：1. the amino acid balance of feed can be adjusted, promote poultry growth；2. meat can be improved；3. can change
The nutritive value of the low feed of kind amino acid digestibility；4. feedstuff cost can be reduced；Therefore in China, European Union member countries and U.S.
Continent country, has been widely used in feedstuff industry.
Currently, the production method of threonine mainly has 3 kinds of fermentation method protein Hydrolyze method and chemical synthesis, microorganism hair
Ferment method produces threonine, because the advantages that its simple process and low cost is honest and clean has become current main stream approach.Domestic threonine at present
The most commonly used is microbe fermentation methods for manufacturer, but there are still fermentation production rate is low, and the shortcomings of cost of material is higher；Soviet Union
The extraction process of propylhomoserin is that threonine fermentation liquid intercepts filtering mycoprotein by ceramic membrane, and ceramic membrane filtrate is concentrated by evaporator and tied
Brilliant technique concentrates threonine fermentation liquid with ceramic membrane filter, due to mycoprotein concentration in zymotic fluid when being concentrated to 5-7 times
Rise, the flux of ceramic membrane is begun to decline, and for further recycling threonine, starts to add water at this moment, amount of water is fermented liquid
Long-pending 20%, zymotic fluid are finally concentrated to 9-10 times, and the filtrate of ceramic membrane carries out condensing crystallizing with four-effect evaporator, finally dries
Packaging.But ceramic membrane, which concentrates, in the technique also contains a certain amount of threonine in 9-10 times of feed liquid, this part threonine can not carry out
The drawbacks of recycling, threonine yield caused to reduce, and also contain certain mycoprotein in feed liquid, cause purity to reduce.
Invention content
In order to solve the above technical problem, the present invention provides the fermentation preparation processes of particle threonine, improve threonine
Yield and extract yield；Technique is prepared into graininess threonine through the invention, can improve mobility, convenient for storage and fortune
Defeated, control solubility, improves the quality and added value of threonine.
This law invention is achieved by the following technical solution：
The fermentation preparation process of particle threonine comprising following steps：
Threonine fermentation liquid first passes around disk plate centrifuge centrifugation, collects supernatant, then passes through ceramic membrane filter, then by filtrate
Concentration is that the one third of original volume is heated to 75 DEG C, then detach through decanter centrifuge then with sulfuric acid tune pH4.2, and removal is heavy
It forms sediment, collects supernatant；Then pass through ultrafiltration membrance filter, filtered solution is collected, by the intermittent single-action condensing crystallizing pot knot of filtered solution
Crystal is collected by centrifugation in crystalline substance, and then 120 DEG C of dryings to moisture are 0.8wt%, compress slabbing, then put into granulation tower,
It is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through being crushed whole grain, pass sequentially through 20 mesh and 50 mesh sieve, sieve
It removed thick, meticulous particle, and collected the granule of target grain size, pack to obtain the final product.
Preferably,
The molecular cut off of the ceramic membrane is 10,000 Da.
Preferably,
The ultrafiltration retaining molecular weight is 300Da.
Preferably,
The centrifugal speed of the disk plate centrifuge is 4000rpm, centrifugation time 5min.
Preferably,
The centrifugal speed of the decanter centrifuge is 5000rpm, centrifugation time 3min.
Preferably,
The preparation method of the threonine fermentation liquid includes the following steps：The inoculation of the Escherichia coli of threonine according to 10% will be produced
Amount, which is linked into the fermentation tank containing fermentation medium, is fermented to obtain threonine fermentation liquid, 32 DEG C of fermentation temperature, and tank pressure is
0.05MPa, air quantity 600L/h, rotating speed 100rpm, fermentation time 96h.
Preferably,
The preparation method of the fermentation medium includes the following steps：
Maize straw is crushed, then with maize peel according to 2：1 mass ratio mixing, then it is 50 mesh to be crushed to grain size, is then added
It is added in the water of 5 times of weight, warming while stirring keeps the temperature 30min to 80 DEG C, then adds potassium dihydrogen phosphate 1g/L, phosphoric acid hydrogen
Dipotassium 1g/L, epsom salt 0.2g/L, ferrous sulfate heptahydrate 0.01g/L, manganese sulfate monohydrate 0.01g/L, stir evenly, cooling
To 32 DEG C, trichoderma culture solution is obtained；Trichoderma reesei is linked into trichoderma culture solution according to 10% inoculum concentration, cultivates 80h, culture
When control mixing speed be 100rpm, cultivation temperature be 32 DEG C；It after the completion of culture, is ultrasonically treated, then it is 55 DEG C to adjust temperature, is added
It is 6 that sulfuric acid, which adjusts pH, is separately added into lysozyme and acid protease, under heat-retaining condition, is digested 12 hours；Then 100 DEG C of enzyme deactivations
5min, last cooled to room temperature to get.
Preferably,
The sonication treatment time is 5min, ultrasonic power 500W.
Preferably,
The additive amount of the lysozyme is 20,000 U：The additive amount of 1L solution, acid protease is 10,000 U：1L is molten
Compared with prior art, it includes mainly the following aspects that the technology of the present invention, which uses above-mentioned technique, advantage,：
Maize straw is handled using trichoderma reesei, Li's Trichoderma is then digested, obtains the training containing reduced sugar and mycoprotein
Base is supported, is used for Escherichia coli, is improved fermentation efficiency, and agricultural wastes are utilized, reduce cost.The present invention utilizes
Ultrasonic wave cavitation, generate partial high pressure high temperature, somatic cells are impacted, cytomorphosis and rupture are caused, it is auxiliary
It helps lysozyme to carry out broken wall dissolving, and is assisted using acid protease, improve thalline sporoderm-broken rate and reach 95% or more；
The mycoprotein liquid of preparation can be used as nitrogen source, reduce cost；
Zymotic fluid prepared by the present invention removes most of thalline through centrifuge first, is then concentrated after ceramic membrane filter, then
It is centrifuged by decanter centrifuge, is mostly the clear liquid containing micro mycoprotein after separation, avoids mycoprotein to ceramics
Film is blocked, and a small amount of pureed mycoprotein is only generated in clear liquid, and clear liquid is filtered by ultrafiltration membrane again, improves threonine
Yield and purity；Powdered threonine is prepared into graininess threonine by the present invention, can improve mobility, convenient for storage and
Transport, control solubility, improve the quality and added value of threonine.
Specific implementation mode
Those skilled in the art can use for reference present disclosure, be suitably modified technological parameter realization.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.The product and method of the present invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified or are suitably changed and combined in bright content, spirit and scope, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
The fermentation preparation process of particle threonine comprising following steps：
Maize straw is crushed, then with maize peel according to 2：1 mass ratio mixing, then it is 50 mesh to be crushed to grain size, is then added
It is added in the water of 5 times of weight, warming while stirring keeps the temperature 30min to 80 DEG C, then adds potassium dihydrogen phosphate 1g/L, phosphoric acid hydrogen
Dipotassium 1g/L, epsom salt 0.2g/L, ferrous sulfate heptahydrate 0.01g/L, manganese sulfate monohydrate 0.01g/L, stir evenly, cooling
To 32 DEG C, trichoderma culture solution is obtained；By trichoderma reesei ATCC 13631 according to 10% inoculum concentration（Inoculum density be 4 ×
106cfu/mL）It is linked into trichoderma culture solution, cultivates 80h, control mixing speed is 100rpm when culture, and cultivation temperature is 32 DEG C；
After the completion of culture, it is ultrasonically treated 5min, ultrasonic power 500W, then it is 55 DEG C to adjust temperature, it is 6 to add sulfuric acid adjustment pH, respectively
Lysozyme and acid protease is added, the additive amount of lysozyme is 20,000 U：The additive amount of 1L solution, acid protease is 10,000 U：
1L solution under heat-retaining condition, digests 12 hours；Then 100 DEG C of enzyme deactivation 5min, last cooled to room temperature are trained to get fermentation
Support base（Content of reducing sugar is 85.9g/L, and albumen and polypeptide total content are 34.7g/L）；
By colibacillus engineering K12 △ dapA seed liquors（1×108cfu/mL）It is linked into containing hair according to 10% inoculum concentration
Ferment in the fermentation tank of ferment culture medium, 32 DEG C of temperature, tank pressure be 0.05MPa, air quantity 600L/h, rotating speed 100rpm,
Fermentation time culture is 96h；Threonine content：12.9g/100ml, residual sugar amount 0.44%.
Threonine fermentation liquid first passes around disk plate centrifuge and centrifuges 5min with 4000rpm, collects supernatant, then by pottery
Porcelain film（10000 Da molecular cut offs）Filtering, then the one third that filtrate concentration is original volume is added then with sulfuric acid tune pH4.2
Heat is detached to 75 DEG C, then through decanter centrifuge, centrifugal speed 5000rpm, centrifugation time 3min, removal precipitation, in collection
Clear liquid（Protein content is less than 0.5%）；Then pass through ultrafiltration membrance filter, collect filtered solution, ultrafiltration retaining molecular weight is 300Da；
The intermittent single-action condensing crystallizing pot of filtered solution is crystallized, crystal is collected by centrifugation, then 120 DEG C of drying to moistures are
0.8%, slabbing is compressed, then put into granulation tower, is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then
Through being crushed whole grain, 20 mesh and 50 mesh sieve are passed sequentially through, thick, meticulous particle was weeded out, and collected the granule of target grain size, are packed
To obtain the final product；Its major parameter is：Threonine total recovery >=90%, purity >=98%, moisture≤0.4%, scorching hot residue≤0.5%, accumulation
Density：0.6-0.7g/ml, granularity：20 mesh oversizes are 18%, 20-50 mesh oversize 76%, 20 mesh screenings 6%.
Comparative example 1
Fermentation medium：The culture medium that patented technology " a kind of ultralow moisture content threonine production method " before applicant is recorded：
Glucose 80g/L, corn steep liquor 20g/L, ammonium sulfate 2g/L, calcium carbonate 0.75g/L, KH2PO40.2g/L, K2HPO4 0.2g/L,
NaCl 0.2g/L, pH value 6.5；Remaining step is the same as embodiment 1.The content of threonine in zymotic fluid：10.1g/100ml, residual sugar amount
0.89%, production amount of threonine is significantly lower than embodiment 1, and residual sugar amount is also above embodiment 1；By calculating, embodiment 1 is fermented into
This is significantly lower than comparative example 1, can be that enterprise saves fermented and cultured cost, improve enterprise profit.
Comparative example 2
Technique does not use supersound process with embodiment 1.Albumen and content of peptides in fermentation medium reduce by 20%, can
Can be because only with the poor reason of lysozyme broken wall dissolving thalline effect, so as to cause effective nitrogen source component in culture medium
It reduces.The content of threonine in zymotic fluid：11.2g/100ml production amount of threonine reduces 15% than embodiment 1.
Finally, it should also be noted that it is listed above be only the present invention several specific embodiments.Obviously, the present invention is not
It is limited to above example, acceptable there are many deformations.Those skilled in the art can be direct from present disclosure
All deformations for exporting or associating, are considered as protection scope of the present invention.
Claims (9)
1. the fermentation preparation process of particle threonine comprising following steps：
Threonine fermentation liquid first passes around disk plate centrifuge centrifugation, collects supernatant, then passes through ceramic membrane filter, then by filtrate
Concentration is that the one third of original volume is heated to 75 DEG C, then detach through decanter centrifuge then with sulfuric acid tune pH4.2, and removal is heavy
It forms sediment, collects supernatant；Then pass through ultrafiltration membrance filter, filtered solution is collected, by the intermittent single-action condensing crystallizing pot knot of filtered solution
Crystal is collected by centrifugation in crystalline substance, and then 120 DEG C of dryings to moisture are 0.8wt%, compress slabbing, then put into granulation tower,
It is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through being crushed whole grain, pass sequentially through 20 mesh and 50 mesh sieve, sieve
It removed thick, meticulous particle, and collected the granule of target grain size, pack to obtain the final product.
2. fermentation preparation process according to claim 1, which is characterized in that the molecular cut off of the ceramic membrane is 10,000
Da。
3. fermentation preparation process according to claim 1, which is characterized in that the ultrafiltration retaining molecular weight is 300Da.
4. fermentation preparation process according to claim 1, which is characterized in that the centrifugal speed of the disk plate centrifuge is
4000rpm, centrifugation time 5min.
5. fermentation preparation process according to claim 1, which is characterized in that the centrifugal speed of the decanter centrifuge is
5000rpm, centrifugation time 3min.
6. fermentation preparation process according to claim 1, which is characterized in that the preparation method packet of the threonine fermentation liquid
Include following steps：The Escherichia coli for producing threonine are linked into according to 10% inoculum concentration in the fermentation tank containing fermentation medium
Fermented to obtain threonine fermentation liquid, 32 DEG C of fermentation temperature, tank pressure is 0.05MPa, air quantity 600L/h, and rotating speed is
100rpm, fermentation time 96h.
7. fermentation preparation process according to claim 6, which is characterized in that the preparation method of the fermentation medium includes
Following steps：
Maize straw is crushed, then with maize peel according to 2：1 mass ratio mixing, then it is 50 mesh to be crushed to grain size, is then added
It is added in the water of 5 times of weight, warming while stirring keeps the temperature 30min to 80 DEG C, then adds potassium dihydrogen phosphate 1g/L, phosphoric acid hydrogen
Dipotassium 1g/L, epsom salt 0.2g/L, ferrous sulfate heptahydrate 0.01g/L, manganese sulfate monohydrate 0.01g/L, stir evenly, cooling
To 32 DEG C, trichoderma culture solution is obtained；Trichoderma reesei is linked into trichoderma culture solution according to 10% inoculum concentration, cultivates 80h, culture
When control mixing speed be 100rpm, cultivation temperature be 32 DEG C；It after the completion of culture, is ultrasonically treated, then it is 55 DEG C to adjust temperature, is added
It is 6 that sulfuric acid, which adjusts pH, is separately added into lysozyme and acid protease, under heat-retaining condition, is digested 12 hours；Then 100 DEG C of enzyme deactivations
5min, last cooled to room temperature to get.
8. fermentation preparation process according to claim 7, which is characterized in that the sonication treatment time is 5min, ultrasound
Power is 500W.
9. fermentation preparation process according to claim 7, which is characterized in that the additive amount of the lysozyme is 20,000 U：1L
The additive amount of solution, acid protease is 10,000 U：1L solution.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109355325A (en) * | 2018-10-18 | 2019-02-19 | 许传高 | The symbiosis production. art of particle threonine and granule protein |
| CN109400410A (en) * | 2018-10-18 | 2019-03-01 | 许传高 | Utilize the method for Threonine Fermentation waste liquid production biological organic matter |
| CN109439702A (en) * | 2018-10-18 | 2019-03-08 | 许传高 | The technique for handling threonine high gravity fermentation waste water |
| CN109486876A (en) * | 2018-12-24 | 2019-03-19 | 齐齐哈尔龙江阜丰生物科技有限公司 | A method of threonine is extracted and is purified in fermentation |
| CN109706195A (en) * | 2018-12-27 | 2019-05-03 | 齐齐哈尔龙江阜丰生物科技有限公司 | The production technology of granular pattern threonine |
| CN110004192A (en) * | 2018-10-17 | 2019-07-12 | 许传高 | A kind of method of preparing granular type threonine |
| CN110051634A (en) * | 2019-05-29 | 2019-07-26 | 绥化象屿金谷生化科技有限公司 | A kind of L-threonine processes for wet granulation |
| CN110885864A (en) * | 2019-12-01 | 2020-03-17 | 齐齐哈尔龙江阜丰生物科技有限公司 | Process for preparing threonine fermentation medium by using corn bran hydrolysate |
| CN110894522A (en) * | 2019-12-01 | 2020-03-20 | 齐齐哈尔龙江阜丰生物科技有限公司 | Corn bran hydrolysate and application thereof in preparation of threonine through fermentation |
| CN110923273A (en) * | 2019-12-02 | 2020-03-27 | 齐齐哈尔龙江阜丰生物科技有限公司 | Method for improving production of threonine by microbial fermentation |
| CN111018732A (en) * | 2019-12-26 | 2020-04-17 | 齐齐哈尔龙江阜丰生物科技有限公司 | Extraction and preparation process of granular threonine |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759580A (en) * | 2008-12-24 | 2010-06-30 | 中国科学院过程工程研究所 | Method for preparing threonine crystal by threonine fermentation liquid |
| CN104313105A (en) * | 2014-09-29 | 2015-01-28 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing 65% threonine by using threonine fermenting liquor and waste mother liquor |
| CN104757273A (en) * | 2015-04-12 | 2015-07-08 | 呼伦贝尔东北阜丰生物科技有限公司 | Process for preparing granular type threonine product |
| CN106554977A (en) * | 2016-06-26 | 2017-04-05 | 宁夏海诚电化信息科技有限公司 | A kind of threonine production technology |
-
2018
- 2018-05-03 CN CN201810414666.XA patent/CN108658798A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759580A (en) * | 2008-12-24 | 2010-06-30 | 中国科学院过程工程研究所 | Method for preparing threonine crystal by threonine fermentation liquid |
| CN104313105A (en) * | 2014-09-29 | 2015-01-28 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing 65% threonine by using threonine fermenting liquor and waste mother liquor |
| CN104757273A (en) * | 2015-04-12 | 2015-07-08 | 呼伦贝尔东北阜丰生物科技有限公司 | Process for preparing granular type threonine product |
| CN106554977A (en) * | 2016-06-26 | 2017-04-05 | 宁夏海诚电化信息科技有限公司 | A kind of threonine production technology |
Non-Patent Citations (1)
| Title |
|---|
| 徐礼生等: "基因工程菌生产氨基酸的研究进展", 《基因组学与应用生物学》 * |
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110004192A (en) * | 2018-10-17 | 2019-07-12 | 许传高 | A kind of method of preparing granular type threonine |
| CN109400410A (en) * | 2018-10-18 | 2019-03-01 | 许传高 | Utilize the method for Threonine Fermentation waste liquid production biological organic matter |
| CN109439702A (en) * | 2018-10-18 | 2019-03-08 | 许传高 | The technique for handling threonine high gravity fermentation waste water |
| CN109355325A (en) * | 2018-10-18 | 2019-02-19 | 许传高 | The symbiosis production. art of particle threonine and granule protein |
| CN109400410B (en) * | 2018-10-18 | 2021-09-17 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for producing biological organic matter by using threonine fermentation waste liquid |
| CN109486876B (en) * | 2018-12-24 | 2021-07-30 | 齐齐哈尔龙江阜丰生物科技有限公司 | Method for fermenting, extracting and purifying threonine |
| CN109486876A (en) * | 2018-12-24 | 2019-03-19 | 齐齐哈尔龙江阜丰生物科技有限公司 | A method of threonine is extracted and is purified in fermentation |
| CN109706195A (en) * | 2018-12-27 | 2019-05-03 | 齐齐哈尔龙江阜丰生物科技有限公司 | The production technology of granular pattern threonine |
| CN109706195B (en) * | 2018-12-27 | 2022-04-08 | 齐齐哈尔龙江阜丰生物科技有限公司 | Production process of granular threonine |
| CN110051634A (en) * | 2019-05-29 | 2019-07-26 | 绥化象屿金谷生化科技有限公司 | A kind of L-threonine processes for wet granulation |
| CN110894522A (en) * | 2019-12-01 | 2020-03-20 | 齐齐哈尔龙江阜丰生物科技有限公司 | Corn bran hydrolysate and application thereof in preparation of threonine through fermentation |
| CN110885864A (en) * | 2019-12-01 | 2020-03-17 | 齐齐哈尔龙江阜丰生物科技有限公司 | Process for preparing threonine fermentation medium by using corn bran hydrolysate |
| CN110923273A (en) * | 2019-12-02 | 2020-03-27 | 齐齐哈尔龙江阜丰生物科技有限公司 | Method for improving production of threonine by microbial fermentation |
| CN111018732A (en) * | 2019-12-26 | 2020-04-17 | 齐齐哈尔龙江阜丰生物科技有限公司 | Extraction and preparation process of granular threonine |
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