CN109706195A - The production technology of granular pattern threonine - Google Patents
The production technology of granular pattern threonine Download PDFInfo
- Publication number
- CN109706195A CN109706195A CN201811606355.XA CN201811606355A CN109706195A CN 109706195 A CN109706195 A CN 109706195A CN 201811606355 A CN201811606355 A CN 201811606355A CN 109706195 A CN109706195 A CN 109706195A
- Authority
- CN
- China
- Prior art keywords
- threonine
- fermentation
- production technology
- granular pattern
- prepares
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention belongs to technical field of amino acid production, disclose the production technology of granular pattern threonine comprising following steps: step 1) prepares fermentation medium, step 2 fermentation, and step 3) is centrifuged, and step 4) prepares granular pattern threonine.Production amount of threonine of the present invention is high, and by-product is less.
Description
Technical field
The invention belongs to amino acids production fields, and in particular to the production technology of granular pattern threonine.
Background technique
Threonine is soluble easily in water and does not dissolve in the organic solvents such as ethyl alcohol, ether, belongs to aliphatic amino acid, slightly sweet, is
Constitute a kind of essential amino acid of the mankind and plant animal protein.Threonine is as one of amino acid necessary to human body, in people
Life in play increasingly important role.With the development of aquaculture, animal and fowl fodder demand increases sharply, ammonia of reviving
Acid, which plays, to be increasingly taken seriously from the role of the nutritional ingredient of external world's intake.Medicine, food and in terms of
All it is widely used.Threonine belongs to one of product of industrial fermentation, and according to statistics, global threonine is supplied within 2017
It should measure and reach 68.5 ten thousand tons, increase by 15.5% on a year-on-year basis, or increase by 9.2 ten thousand tons, the 80% of increment is from China.2017
The supply of Chinese threonine reaches 53.5 ten thousand tons, increases by 15.6% on a year-on-year basis, accounts for the 78% of world market.Since 2013,
Global threonine supply is averaged compound growth rate 13.4%, than -2013 years 2003 between speedup be dropped by nearly half.2017
Year Chinese threonine manufacturing enterprise is based on plum blossom, Fu Feng, Yi Pin, Cheng Fu, great achievement, uncommon outstanding supplement；International manufacturing enterprise is main
With aginomoto and ADM.Chinese threonine exports 37.4 ten thousand tons within 2017, accounts for the 69.9% of yield, domestic supply 16.1
Ten thousand tons, 130,000 tons of domestic demand.
Colibacillus engineering strain is the main bacterial strain of microbe industrial fermentation production threonine, generates L-threonine in fermentation
While, the metabolic by-products such as acetic acid, alanine, valine and arginine can be also generated, it is raw to influence thallus to a certain extent
Long and L-threonine synthesis and accumulation.Wherein, the inhibitory effect of acetic acid is particularly evident, when Acetic Acid Accumulation is to a certain concentration,
The specific growth rate of thallus declines rapidly, and Product formation substantially reduces, and forms vicious circle, while the expression of foreign gene
It is severely impacted.Acetic Acid Accumulation is adversely affected caused by cell metabolism, is outer using Escherichia coli as host strain expression
One very important problem of source protein.How the content of acetic acid is reduced, to improve biomass and production amount of threonine is
The emphasis that we study.Document " control of acetic acid in L-threonine fermentation process, science and technology of fermenting communication 2012 " is in large intestine bar
During bacterium fermentation prepares threonine, the generation of its by-product acetic acid is controlled by selecting suitable fermentation condition, can be dropped
The generation of low acetate, but reduce amplitude and be reduced to unobvious, the yield of threonine cannot be greatly improved, there is no industrialization promotions
Value.The culture medium that patented technology " a kind of ultralow moisture content threonine production method " before applicant is recorded: glucose
80g/L, corn pulp 20g/L, ammonium sulfate 2g/L, calcium carbonate 0.75g/L, KH2PO40.2g/L, K2HPO4 0.2g/L, NaCl
0.2g/L, pH value 6.5；The content of threonine can reach 10g/100ml in fermentation liquid, but the phase is dead after fermentation for bacterial strain
Rate is higher, and glucose utilization is larger, also there is to be hoisted threonine yield in fermentation liquid.On this basis, applicant after
It is continuous to improve, a kind of method of preparing granular type threonine is proposed, fermentation, can by being inoculated with Chlamydomonas reinhardtii in fermentation
Carry out non-light and effect as carbon source using the acetic acid in fermentation liquid, and it is more difficult use glycerol as carbon source, to relieve pair
The inhibiting effect of Escherichia coli production threonine, additionally it is possible to carry out micro photosynthesis release oxygen, be produced for Escherichia coli fermentation
Threonine come using；By adding Chlamydomonas reinhardtii, it is not only able to improve the yield of threonine, and mycoprotein yield is also corresponding
It improves.But bacterial strain mixed fermentation technology is more difficult to control, fermentation costs improve, and the later period separates product, and there is also certain difficulties.
It needs that this method is further improved.
Summary of the invention
In order to solve the defects of prior art, the invention proposes the production technologies of granular pattern threonine.Present invention process
It can be improved granular pattern production amount of threonine, simple process is feasible, has a extensive future.
The present invention is achieved by the following technical solution:
The production technology of granular pattern threonine comprising following steps: step 1) prepares fermentation medium, step 2 fermentation, step
Rapid 3) to be centrifuged, step 4) prepares granular pattern threonine.
Further, the step 1) prepares fermentation medium, includes the following steps:
Step 1) prepares fermentation medium: glucose 20-30g/L, glycerol 15-20g/L, corn pulp 15-20g/L, ammonium sulfate
1.5-2g/L, potassium dihydrogen phosphate 0.1-0.2g/L, dipotassium hydrogen phosphate 0.1-0.2g/L, epsom salt 0.1-0.2g/L, seven water
Ferrous sulfate 5-10mg/L, manganese sulfate monohydrate 5-10mg/L, isoleucine 50-400mg/L, fulvic acid 5-40mg/L, pH value
5.5-6.0。
Further, the step 2 fermentation, includes the following steps: the colibacillus engineering seed liquor that will produce threonine
It is linked into the fermentor containing fermentation medium and ferments according to the inoculum concentration of 10-12%, 30 DEG C of temperature, tank pressure is 0.03-
Then 0.04MPa, ventilatory capacity 0.4-0.6vvm, revolving speed 100rpm, fermentation time 30-36h add inositol 100-
200mg/L continues fermented and cultured 24-30h, controls sugar content between 0.3-0.5% by stream plus glucose solution, stops hair
Ferment collects fermentation liquid；In entire fermentation process, controlling pH by Feeding ammonia water is 5.5-6.0.
Further, step 3) centrifugation, include the following steps: fermentation liquid first pass around disk plate centrifuge with
4000rpm is centrifuged 5min, collects supernatant liquid；
Further, the step 4) prepares granular pattern threonine, includes the following steps: to pass through supernatant liquid obtained by step 3)
Ceramic membrane filter collects filtered solution, by filtered solution by being centrifuged, collects supernatant；Then pass through ultrafiltration membrance filter, collect ultrafiltration
Liquid；Ultrafiltrate is subjected to condensing crystallizing, crystal is collected by centrifugation, then drying to moisture content is 0.8%, slabbing is compressed, then
It puts into granulation tower, is in fluidized state under the action of thermal current；Fluidized bed drying, then through broken whole grain, sieving obtains particle
Type threonine.
Preferably, in the fermentation medium, isoleucine 200-400mg/L, fulvic acid 10-20mg/L.
Preferably, the molecular cut off of the ceramic membrane is 10,000 Da.
Preferably, the molecular cut off of the ultrafiltration membrane is 300Da.
The beneficial effect of starting point and acquirement that the present invention studies mainly includes but is not limited to the following aspects:
Fermenting carbon source selection glucose and glycerol of the present invention, earlier fermentation, cell density is low, and oxygen-supplying amount is sufficient, and Escherichia coli are excellent
First with glucose as carbon source, the generation of growing microorganism and threonine can be promoted；It ferments the middle and later periods, concentration of glucose drop
Low, Escherichia coli use glycerol as carbon source at this time, since the rate that cell absorbs glycerol is lower, under the carbon flow of glycolysis
Drop, to reduce the accumulation of acetic acid, while improving the yield of threonine；
Oxaloacetic acid derive from tricarboxylic acid cycle, the main path of oxaloacetic acid is to flow to glutamic acid route of synthesis, can by pair
The feedback inhibition of the approach improves production amount of threonine；Earlier fermentation is conducive to paddy ammonia by adjusting the pH slant acidity of fermentation liquid
The synthesis of acid, and makes cell permeability be deteriorated, and glutamic acid will not flow out extracellular, is conducive to Rapid Accumulation glutamic acid,
To generate feedback inhibition to glutamic acid route of synthesis within a short period of time, so that oxaloacetic acid flows to aspartic acid way earlier
Diameter；But pH is too low, is unfavorable for neutralizing the by-products such as acetic acid, lactic acid, it is suitable for selecting 5.5-6.
Oxaloacetic acid produces aspartic acid by transamination, and then synthesizes threonine, and threonine can be closed further
At isoleucine；Feedback inhibition can be carried out by adding isoleucine in the fermentation medium, so that threonine be promoted to accumulate.
Appropriate fulvic acid is added in fermentation medium, in containing the groups such as a large amount of phenolic hydroxyl groups, carbonyl, electrolysis degree is higher,
It can promote to utilize O during Amino acid synthesis2As hydrogen acceptor, and then pyruvic acid is reduced as hydrogen acceptor, therefore lactic acid etc.
By-product production quantity is reduced.Suitable inositol is added in fermentation process, CO can be strengthened2Fixed reaction weakens glyoxalic acid and follows
Ring improves the yield of amino acid.
Powdered threonine is prepared into granular pattern threonine by the present invention, can improve mobility, convenient for preservation and transport,
Solubility is controlled, the quality and added value of threonine are improved.
Figure of description
Fig. 1: influence of the fermentation factor to threonine content in fermentation liquor；
Fig. 2: influence of the fermentation factor to acetic acid content in fermentation liquid；
Fig. 3: influence of the fermentation factor to Lactic Acid from Fermentation Broth content；
Fig. 4: influence of the additive amount of isoleucine in the medium to threonine content in fermentation liquor；
Fig. 5: influence of the additive amount of fulvic acid in the medium to threonine content in fermentation liquor.
Specific embodiment
Those skilled in the art can use for reference present disclosure, be suitably modified realization of process parameters.In particular, it should be pointed out that
All similar substitutions and modifications are apparent to those skilled in the art, they are considered as being included in this hair
It is bright.Product and method of the invention is described by preferred embodiment, and related personnel can obviously not depart from this hair
Product as described herein and method are modified in bright content, spirit and scope or appropriate changes and combinations, to realize and answer
Use the technology of the present invention.For a further understanding of the present invention, the following describes the present invention in detail with reference to examples.
Embodiment 1
The production technology of granular pattern threonine comprising following steps:
Step 1) prepares fermentation medium: glucose 25g/L, glycerol 20g/L, corn pulp 20g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.1g/L, dipotassium hydrogen phosphate 0.1g/L, epsom salt 0.1g/L, ferrous sulfate heptahydrate 5mg/L, manganese sulfate monohydrate
5mg/L, isoleucine 200mg/L, fulvic acid 10mg/L, pH value 5.5；
Step 2 fermentation: by colibacillus engineering K12 △ dapA seed liquor, (concentration of seed liquor is 1 × 108Cfu/mL it) presses
It is linked into the fermentor containing fermentation medium and ferments according to 12% inoculum concentration, 30 DEG C of temperature, tank pressure is 0.03MPa, is led to
Tolerance is 0.4vvm, revolving speed 100rpm, fermentation time 36h, then adds inositol 100mg/L, continues fermented and cultured for 24 hours,
Controlling sugar content by the glucose solution of stream plus 100g/L is 0.5%, stops fermentation, collects fermentation liquid；Entire fermentation process
In, controlling pH by the ammonium hydroxide of stream plus 30% is 5.5；
Step 3) centrifugation: fermentation liquid first passes around disk plate centrifuge and is centrifuged 5min with 4000rpm, collects supernatant liquid and precipitating；
Step 4) prepares granular pattern threonine: supernatant liquid is filtered by ceramic membrane (10,000 Da molecular cut off), collects filtration
Liquid separates filtered solution through decanter centrifuge, centrifugal speed 5000rpm, centrifugation time 3min, collects supernatant (albumen
Content is less than 0.5%)；Then pass through ultrafiltration membrance filter, collect ultrafiltrate, ultrafiltration retaining molecular weight is 300Da；By ultrafiltrate
It is crystallized with intermittent single-action condensing crystallizing pot, crystal is collected by centrifugation, then 120 DEG C of dryings to moisture content are 0.8%, are compressed into
Sheet, then put into granulation tower, it is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through being crushed whole grain, according to
It is secondary to pass through 20 mesh and 50 meshes, it weeded out thick, meticulous particle, and collected the granular pattern threonine of target grain size, pack to obtain the final product.
Embodiment 2
The production technology of granular pattern threonine comprising following steps:
Step 1) prepares fermentation medium: glucose 30g/L, glycerol 15g/L, corn pulp 15g/L, ammonium sulfate 2g/L, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.2g/L, dipotassium hydrogen phosphate 0.2g/L, epsom salt 0.2g/L, ferrous sulfate heptahydrate 10mg/L, manganese sulfate monohydrate
10mg/L, isoleucine 300mg/L, fulvic acid 20mg/L, pH value 5.5；
Step 2 fermentation: by colibacillus engineering K12 △ dapA seed liquor, (concentration of seed liquor is 1 × 108Cfu/mL it) presses
It is linked into the fermentor containing fermentation medium and ferments according to the inoculum concentration of 10-12%, 30 DEG C of temperature, tank pressure is
Then 0.04MPa, ventilatory capacity 0.6vvm, revolving speed 100rpm, fermentation time 32h add inositol 200mg/L, after supervention
Ferment culture 28h, controlling sugar content by the glucose solution of stream plus 100g/L is 0.4%, stops fermentation, collects fermentation liquid；Entirely
In fermentation process, controlling pH by the ammonium hydroxide of stream plus 20-30% is 6.0；
Step 3) centrifugation: fermentation liquid first passes around disk plate centrifuge and is centrifuged 5min with 4000rpm, collects supernatant liquid and precipitating；
Step 4) prepares granular pattern threonine: supernatant liquid is filtered by ceramic membrane (10,000 Da molecular cut off), collects filtration
Liquid separates filtered solution through decanter centrifuge, centrifugal speed 5000rpm, centrifugation time 3min, collects supernatant (albumen
Content is less than 0.5%)；Then pass through ultrafiltration membrance filter, collect ultrafiltrate, ultrafiltration retaining molecular weight is 300Da；By ultra filtration
Liquid is crystallized with intermittent single-action condensing crystallizing pot, and crystal is collected by centrifugation, and then 120 DEG C of dryings to moisture content are 0.8%, compression
Slabbing, then put into granulation tower, it is in fluidized state under the action of thermal current；65 DEG C of fluidized bed dryings, then through being crushed whole grain,
It passes sequentially through 20 mesh and 50 meshes, weeded out thick, meticulous particle, collect the granular pattern threonine of target grain size, pack to obtain the final product.
Embodiment 3
Influence of the different factors to by-products contents such as production amount of threonine, acetic acid and lactic acid in zymotechnique of the present invention.
Group is set:
Experimental group: embodiment 1；
Control group 1: not adding isoleucine, remaining is the same as embodiment 1；
Control group 2: not adding fulvic acid, remaining is the same as embodiment 1；
Control group 3: not adding inositol, remaining is the same as embodiment 1；
Glycerol: being replaced with the glucose of equal quality by control group 4, remaining is the same as embodiment 1.
The content of threonine, acetic acid and lactic acid is as shown in Figs. 1-3 in each final fermentation liquid of group.It is compareed by setting
Group has detected influence of the fermentation factors such as glycerol, fulvic acid, isoleucine and inositol to threonine, acetic acid and lactic acid；It is logical
Fig. 1-3 is crossed as it can be seen that the fermentation factors such as glycerol, fulvic acid, isoleucine and inositol have positive influence to the yield of threonine,
Wherein, inositol influences maximum, and fulvic acid and isoleucine take second place, and the influence of glycerol is minimum；But influence of the glycerol to by-product
Larger, control group 4 does not add glycerol, and the content of by-product acetic acid and lactic acid is highest；And isoleucine is to by-product acetic acid
Influence it is smaller, influence of the fulvic acid to by-product lactic acid is smaller；Factor based on the above effects optimizes fermentation condition,
It is able to ascend the yield of threonine, by-product also accordingly reduces.
Embodiment 4
Influence of the additive amount of isoleucine, fulvic acid in the medium to threonine content in fermentation liquor.
The additive amount of isoleucine is respectively set to 0,50,100,200,400,800(mg/L)；As shown in figure 4, with different
The increase of leucine additive amount, the metabolic pathway for generating isoleucine to threonine generate certain feedback effect, lead to ammonia of reviving
Acid accumulation rate is accelerated, and after increasing to the additive amount of 200mg/L, amplification slows down, after increasing to 400mg/L, threonine content
Do not obviously increase.
The additive amount of fulvic acid is respectively set to 0,5,10,20,40,80(mg/L)；As shown in figure 5, as fulvic acid adds
The increase of dosage promotes to utilize O during Amino acid synthesis2As hydrogen acceptor, and then reduce pyruvic acid as hydrogen acceptor, therefore
The by-products production quantity such as lactic acid is reduced, and the corresponding yield of threonine promoted, and continues growing the yield of fulvic acid to 20mg/L
Afterwards, the content of threonine does not have significant change.
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, though
The right present invention has been described by way of example and in terms of the preferred embodiments, however, being not intended to limit the invention, any technology people for being familiar with this profession
Member can make a little change or modification using the technology contents disclosed certainly without departing from the scope of the present invention, at
For the equivalent embodiment of equivalent variations, but anything that does not depart from the technical scheme of the invention content, according to the technical essence of the invention
Any simple modification, equivalent change and modification to the above embodiments, belong in the range of technical solution of the present invention.
Claims (8)
1. the production technology of granular pattern threonine comprising following steps: step 1) prepares fermentation medium, and step 2 is fermented,
Step 3) centrifugation, step 4) prepare granular pattern threonine.
2. production technology according to claim 1, which is characterized in that the step 1) prepares fermentation medium, including such as
Lower step:
Step 1) prepares fermentation medium: glucose 20-30g/L, glycerol 15-20g/L, corn pulp 15-20g/L, ammonium sulfate
1.5-2g/L, potassium dihydrogen phosphate 0.1-0.2g/L, dipotassium hydrogen phosphate 0.1-0.2g/L, epsom salt 0.1-0.2g/L, seven water
Ferrous sulfate 5-10mg/L, manganese sulfate monohydrate 5-10mg/L, isoleucine 50-400mg/L, fulvic acid 5-40mg/L, pH value
5.5-6.0。
3. production technology according to claim 2, which is characterized in that the step 2 fermentation includes the following steps: to produce
The colibacillus engineering seed liquor of threonine is linked into the fermentor containing fermentation medium according to the inoculum concentration of 10-12%
It ferments, 30 DEG C of temperature, tank pressure is 0.03-0.04MPa, ventilatory capacity 0.4-0.6vvm, revolving speed 100rpm, fermentation time
For 30-36h；Then inositol 100-200mg/L is added, fermented and cultured 24-30h is continued, is contained by stream plus glucose solution control
Sugar amount stops fermentation between 0.3-0.5%, collects fermentation liquid；In entire fermentation process, controlling pH by Feeding ammonia water is
5.5-6.0。
4. production technology according to claim 3, which is characterized in that the step 3) centrifugation includes the following steps: to ferment
Liquid first passes around disk plate centrifuge and is centrifuged 5min with 4000rpm, collects supernatant liquid.
5. production technology according to claim 4, which is characterized in that the step 4) prepares granular pattern threonine, including
Following steps: passing through ceramic membrane filter for supernatant liquid obtained by step 3), collect filtered solution, by filtered solution by being centrifuged, collects
Supernatant；Then pass through ultrafiltration membrance filter, collect ultrafiltrate；Ultrafiltrate is subjected to condensing crystallizing, crystal is collected by centrifugation, then does
Dry to moisture content is 0.8%, compresses slabbing, then puts into granulation tower, is in fluidized state under the action of thermal current；Fluidisation
Bed is dry, then through broken whole grain, sieving obtains granular pattern threonine.
6. according to claim 2-6 be allowed to one described in production technology, which is characterized in that in the fermentation medium, different bright ammonia
Acid is 200-400mg/L, fulvic acid 10-20mg/L.
7. production technology according to claim 6, which is characterized in that the molecular cut off of the ceramic membrane is 10,000 Da.
8. production technology according to claim 6, which is characterized in that the molecular cut off of the ultrafiltration membrane is 300Da.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811606355.XA CN109706195B (en) | 2018-12-27 | 2018-12-27 | Production process of granular threonine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811606355.XA CN109706195B (en) | 2018-12-27 | 2018-12-27 | Production process of granular threonine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109706195A true CN109706195A (en) | 2019-05-03 |
| CN109706195B CN109706195B (en) | 2022-04-08 |
Family
ID=66258624
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811606355.XA Active CN109706195B (en) | 2018-12-27 | 2018-12-27 | Production process of granular threonine |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109706195B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110051634A (en) * | 2019-05-29 | 2019-07-26 | 绥化象屿金谷生化科技有限公司 | A kind of L-threonine processes for wet granulation |
| CN110846349A (en) * | 2019-08-22 | 2020-02-28 | 赵兰坤 | Preparation method of feed additive threonine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104203227A (en) * | 2012-03-07 | 2014-12-10 | 南彻森英国有限公司 | Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria |
| CN106554977A (en) * | 2016-06-26 | 2017-04-05 | 宁夏海诚电化信息科技有限公司 | A kind of threonine production technology |
| CN106967224A (en) * | 2017-04-19 | 2017-07-21 | 兰州理工大学 | Vinasse fulvic acid and its extracting method and application |
| CN108658798A (en) * | 2018-05-03 | 2018-10-16 | 齐齐哈尔龙江阜丰生物科技有限公司 | The fermentation preparation process of particle threonine |
| CN110004192A (en) * | 2018-10-17 | 2019-07-12 | 许传高 | A kind of method of preparing granular type threonine |
-
2018
- 2018-12-27 CN CN201811606355.XA patent/CN109706195B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104203227A (en) * | 2012-03-07 | 2014-12-10 | 南彻森英国有限公司 | Fulvic acid and antibiotic combination for the inhibition or treatment of multi-drug resistant bacteria |
| CN106554977A (en) * | 2016-06-26 | 2017-04-05 | 宁夏海诚电化信息科技有限公司 | A kind of threonine production technology |
| CN106967224A (en) * | 2017-04-19 | 2017-07-21 | 兰州理工大学 | Vinasse fulvic acid and its extracting method and application |
| CN108658798A (en) * | 2018-05-03 | 2018-10-16 | 齐齐哈尔龙江阜丰生物科技有限公司 | The fermentation preparation process of particle threonine |
| CN110004192A (en) * | 2018-10-17 | 2019-07-12 | 许传高 | A kind of method of preparing granular type threonine |
Non-Patent Citations (4)
| Title |
|---|
| 周茜等: "增强乙醛酸循环对大肠杆菌合成L-苏氨酸的影响", 《现代食品科技》 * |
| 梁媛: "糖酵解途径和转运系统的改造对大肠杆菌发酵L-苏氨酸的影响", 《中国优秀硕士学位论文全文数据库（电子期刊） 工程科学I辑》 * |
| 马占龙: "强化CO2固定反应提高发酵转化率的探讨", 《发酵科技通讯》 * |
| 高金岗等: "生化黄腐酸抑菌作用的研究", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110051634A (en) * | 2019-05-29 | 2019-07-26 | 绥化象屿金谷生化科技有限公司 | A kind of L-threonine processes for wet granulation |
| CN110846349A (en) * | 2019-08-22 | 2020-02-28 | 赵兰坤 | Preparation method of feed additive threonine |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109706195B (en) | 2022-04-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11198890B2 (en) | Preparation of (R)-3-hydroxybutyric acid or its salts by one-step fermentation | |
| CN102174449B (en) | Method for producing high-yield gamma-propalanine and application thereof | |
| CN105368766B (en) | One plant of method for producing the genetic engineering bacterium of pentanediamine and its preparing pentanediamine | |
| CN109486876A (en) | A method of threonine is extracted and is purified in fermentation | |
| CN109439702A (en) | The technique for handling threonine high gravity fermentation waste water | |
| CN109706195A (en) | The production technology of granular pattern threonine | |
| CN109609566A (en) | A method of improving production amount of threonine | |
| CN102978252A (en) | L-tryptophan fed-batch fermentation technology | |
| CN109439703A (en) | A kind of culture medium for Threonine Fermentation technique | |
| CN109593797A (en) | A kind of method of fermenting and producing γ-aminobutyric acid | |
| CN112322673B (en) | Fermentation method of glutamic acid | |
| CN107058414B (en) | Method for preparing L-alanine | |
| KR20160112227A (en) | Method for production of GABA with lactic acid bacteria through L-glutamic acid feeding | |
| CN105368887B (en) | A kind of fermentation manufacturing technique of epsilon-poly-L-lysine | |
| CN109161507B (en) | Corynebacterium glutamicum capable of producing L-ornithine at high yield and application thereof | |
| CN110004192A (en) | A kind of method of preparing granular type threonine | |
| CN107988288B (en) | Method for producing propionibacterium bacteriocin through high-density fermentation | |
| JP2018531602A (en) | Bio-based N-acetyl-L-methionine and uses thereof | |
| CN109943511A (en) | One plant of brevibacterium flavum for producing Valine and its application | |
| CN110396530A (en) | A method of improving production amount of threonine and yield | |
| CN1807592A (en) | Method for producing lactic acid bacteria formulation and lactic acid bacteria bacteriocin using ethanol draff liquid | |
| KR20170089451A (en) | Method for producing dry yeast containing selenium using fermentation | |
| CN109355325A (en) | The symbiosis production. art of particle threonine and granule protein | |
| CN101892172A (en) | Bare-glass fly arthrobacter strain and method for producing feeding proteins by using bare-glass fly arthrobacter strain to decompose gossypol | |
| CN110923275A (en) | Fermentation and extraction process of glutamic acid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |