CN106554977A - A kind of threonine production technology - Google Patents
A kind of threonine production technology Download PDFInfo
- Publication number
- CN106554977A CN106554977A CN201610469830.8A CN201610469830A CN106554977A CN 106554977 A CN106554977 A CN 106554977A CN 201610469830 A CN201610469830 A CN 201610469830A CN 106554977 A CN106554977 A CN 106554977A
- Authority
- CN
- China
- Prior art keywords
- threonine
- fermentation
- feedstuff
- seed
- tank
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
- C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
Landscapes
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of feedstuff production field, especially threonine production field.Threonine（That is L threonine）, 2 amino of chemical name, 3 hydroxybutyric acid because which is similar to that threose, therefore is named as threonine.Threonine is a kind of indispensable necessary aminoacid of ecological body, is mainly used in the aspects such as medicine, chemical reagent, food additive, feed additive.Especially the consumption in terms of feed additive increases quick, and feedstuff industry has become the maximum application of threonine requirement amount.
Description
Technical field
The present invention relates to a kind of feedstuff production field, especially threonine production field.
Background technology
Threonine（That is L-Threonine）, chemical name 2 amino 3 hydroxybutyric acid, because which is similar to that threose, therefore life
Entitled threonine.Threonine is a kind of indispensable necessary aminoacid of ecological body, is mainly used in medicine, chemical reagent, food
The aspects such as hardening agent, feed additive.Especially the consumption in terms of feed additive increases quick, and feedstuff industry has become Soviet Union
The application that propylhomoserin has the call.Threonine is added in the mixed feed of teenage piglet and poultry, is pig feed
3rd limiting amino acid of the second limiting amino acid and poultry feed.Threonine has amino in adjustment feedstuff in making an addition to feedstuff
Acid balance, save protein resource, reduce feedstuff cost, improve human body immune function, improve the functions such as poultry meat.
For a long time, international market continues steady-state growth to the demand of aminoacid, is the most fast aminoacid product of demand growth
One of kind.European overwhelming majority raising family is world market Soviet Union with the less Semen Tritici aestivi of threonine content as feedstuff, therefore for many years
Propylhomoserin production can not meet the market demand, need from the large quantities of imports of China every year.
Threonine is corn deep processing high value added product, and production can be given birth to using the method such as fermentation method and chemosynthesis
Produce.Mostly adopt fermentative Production at present in the world, be mainly obtained by raw material of casein, fibroin, sericin etc..With Portugal
Grape sugar and yeast powder and Semen Maydis pulp, add a small amount of adjuvant fermented and prepared.
The content of the invention
The threonine technological process of production
It is inoculated in 38 DEG C of inclined-plane to cultivate 24 hours after Threonine Fermentation strain is activated, then through shaking flask under the conditions of 38 DEG C
Culture was inoculated in seed tank after 24 hours.Pipeline and medium sterilization will first be carried out before seed tank inoculation.By bacterial suspension inoculation
To first class seed pot in divulge information stir culture, stir culture of then divulging information in secondary seed tank can be connect after microscopy is normal
Enter in fermentation tank.Sweat flows sugaring and liquefied ammonia supplementary carbon source and nitrogen source, cocurrent liquid feeding ammonia adjust pH it is stable 6 ~ 7 it
Between, after fermenting 40 ~ 48 hours, when residual sugar is down to below 1%, you can be considered as fermentation termination, put tank, membrane filtration, filter vacuum are dense
2-3 is wrapped in contracting, vacuum crystallization, drying.
Technological process technological process is as follows：Strain inclined plane-→ shake-flask seed-→ seed tank spreads cultivation-and → fermentation tank sends out
Ferment-→ fermentation liquid keep in-→ ceramic membrane filter-→ ultrafilter membrane remove impurity-→ concentration-→ stirred crystallization-→ centrifugation-→
Drying-→ finished product screen subpackage is filled.
Technical process is summarized as follows：
（1）It is prepared by seed
It is inoculated in 38 DEG C of inclined-plane to cultivate 24 hours after Threonine Fermentation strain is activated, then through shaking flask under the conditions of 38 DEG C
Culture was inoculated in seed tank after 24 hours.Pipeline and medium sterilization will first be carried out before seed tank inoculation.By bacterial suspension inoculation
To first class seed pot in divulge information stir culture, stir culture of then divulging information in secondary seed tank can be connect after microscopy is normal
Enter in fermentation tank.
（2）Fermentation
Raw materials of glucose, Semen Maydis pulp, inorganic salt etc. are eliminated through dispensing, even and after bacterium, enters fermentation tank, be inoculated with seed fermentation liquid
After fermented.The sugaring of sweat stream and liquefied ammonia supplementary carbon source and nitrogen source, adjust pH stable between 6-8.Pass through in fermentation
Circulation water management fermentation temperature, and continuous supplementation filtrated air.Sweat terminates fermentation after continuing 30-32 hours.
（3）Filtering fermentation liquor
The impurity such as thalline are removed using " inorganic ceramic film cross flow filter " technology, sheet frame, vacuum drum, centrifuge, diatom is compared
The tradition solid-liquid separation technique such as native machine, with separate thoroughly, fermentation liquid is pure, subsequent extracted is simple, with water displacement is little, product
The features such as high income.Thalline is through filter-press dehydration.
（4）Ultrafiltration
Activated carbon decolorizing in traditional zymotic technique be instead of using organic ceramic ultrafiltration through membranes equipment to filter, ion exchange chromatography tree
The operations such as fat, further clarified broth remove heteroion, and the selection of filter plant greatly reduces blowdown flow rate, and significantly
Improve product quality and yield.
（5）Condensing crystallizing
Using stirred crystallization technology, control to form little particle crystal by crystallization processes, the white product of content height, color and luster can be obtained
Product.
（6）Centrifugation
Dry to separate using flat scraper conveyer centrifugal and remove crystalline mother solution, deionized water washes crystal in separation process
The impurity on surface.Cleanout fluid and crystalline mother solution Jing are concentrated again, Crystallization Procedure further extracts product, and whole mother liquor concentrations are used as liquid
Fertilizer is taken out.After crystal threonine drying, screening, qualified entrance packaging process is checked.
（7）Packaging
Packaging adopts automation mechanized operation, product to store into warehouse for finished product Jing after outward appearance detection is qualified.
Claims (2)
1. the present invention relates to principle composition and proportioning.
2. the present invention relates to technological process technological process：Strain inclined plane-→ shake-flask seed-→ seed tank spreads cultivation-→ fermentation tank
Fermentation-→ fermentation liquid keep in-→ ceramic membrane filter-→ ultrafilter membrane remove impurity-→ concentration-→ stirred crystallization-→ centrifugation-
→ drying-→ finished product screen subpackage is filled.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610469830.8A CN106554977A (en) | 2016-06-26 | 2016-06-26 | A kind of threonine production technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610469830.8A CN106554977A (en) | 2016-06-26 | 2016-06-26 | A kind of threonine production technology |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106554977A true CN106554977A (en) | 2017-04-05 |
Family
ID=58417369
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610469830.8A Pending CN106554977A (en) | 2016-06-26 | 2016-06-26 | A kind of threonine production technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106554977A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108658798A (en) * | 2018-05-03 | 2018-10-16 | 齐齐哈尔龙江阜丰生物科技有限公司 | The fermentation preparation process of particle threonine |
| CN109706195A (en) * | 2018-12-27 | 2019-05-03 | 齐齐哈尔龙江阜丰生物科技有限公司 | The production technology of granular pattern threonine |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1865230A (en) * | 2006-06-14 | 2006-11-22 | 天津科技大学 | Method for extracting L-threonine from fermentation liquor |
| CN101293847A (en) * | 2007-04-29 | 2008-10-29 | 长春大成实业集团有限公司 | Method for extracting threonine with threonine fermentation liquor |
| CN101759580A (en) * | 2008-12-24 | 2010-06-30 | 中国科学院过程工程研究所 | Method for preparing threonine crystal by threonine fermentation liquid |
| CN104313105A (en) * | 2014-09-29 | 2015-01-28 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing 65% threonine by using threonine fermenting liquor and waste mother liquor |
| CN104757273A (en) * | 2015-04-12 | 2015-07-08 | 呼伦贝尔东北阜丰生物科技有限公司 | Process for preparing granular type threonine product |
-
2016
- 2016-06-26 CN CN201610469830.8A patent/CN106554977A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1865230A (en) * | 2006-06-14 | 2006-11-22 | 天津科技大学 | Method for extracting L-threonine from fermentation liquor |
| CN101293847A (en) * | 2007-04-29 | 2008-10-29 | 长春大成实业集团有限公司 | Method for extracting threonine with threonine fermentation liquor |
| CN101759580A (en) * | 2008-12-24 | 2010-06-30 | 中国科学院过程工程研究所 | Method for preparing threonine crystal by threonine fermentation liquid |
| CN104313105A (en) * | 2014-09-29 | 2015-01-28 | 呼伦贝尔东北阜丰生物科技有限公司 | Method for preparing 65% threonine by using threonine fermenting liquor and waste mother liquor |
| CN104757273A (en) * | 2015-04-12 | 2015-07-08 | 呼伦贝尔东北阜丰生物科技有限公司 | Process for preparing granular type threonine product |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108658798A (en) * | 2018-05-03 | 2018-10-16 | 齐齐哈尔龙江阜丰生物科技有限公司 | The fermentation preparation process of particle threonine |
| CN109706195A (en) * | 2018-12-27 | 2019-05-03 | 齐齐哈尔龙江阜丰生物科技有限公司 | The production technology of granular pattern threonine |
| CN109706195B (en) * | 2018-12-27 | 2022-04-08 | 齐齐哈尔龙江阜丰生物科技有限公司 | Production process of granular threonine |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108841758B (en) | Corynebacterium glutamicum mutant strain and application thereof in L-leucine production | |
| CN108299278B (en) | Method for extracting and separating L-tryptophan | |
| CN101182282A (en) | Method for separating and purifying erythritol from fermentation liquor | |
| CN102352393A (en) | Process for producing erythritol by utilizing Yarrowia lipolytica strains | |
| CN104313105B (en) | The method that a kind of utilization threonine fermentation liquid and waste liquor prepare 65% threonine | |
| CN110904163A (en) | Method for improving lactic acid content of corn steep liquor | |
| CN106554977A (en) | A kind of threonine production technology | |
| CN107201384A (en) | A kind of method of separation and Extraction D-ALPHA-Hydroxypropionic acid in sodium zymotic fluid from D-ALPHA-Hydroxypropionic acid | |
| CN109136299B (en) | Method for preparing, extracting and purifying threonine | |
| CN103695490B (en) | High-purity arginine production process | |
| CN111607622B (en) | Process method for producing 3-hydroxy butanone by using wheat B starch | |
| CN103966290B (en) | Yeast protein peptone and preparation method thereof | |
| CN109082449A (en) | A kind of L-Histidine production technology based on plant source | |
| CN108065026A (en) | A kind of preparation method of cereal feedstuff | |
| CN111100823B (en) | Polymyxin B sulfate production strain, preparation method and application of polymyxin B sulfate | |
| CN113545470A (en) | Production method for high-quality monosodium glutamate | |
| CN109182407A (en) | A kind of tryptophan preparation method and its fermentation medium and tryptophan that use fermentation special nutritional member | |
| CN105837460A (en) | Clean production process for separating and extracting glutamic acid from molasses fermented glutamic acid fermentation liquid | |
| CN112143770A (en) | Rhodotorula benthica and application thereof in production of beta-carotene by taking straws as raw material | |
| CN110483318A (en) | A kind of extracting method of L-threonine | |
| CN115141110B (en) | Continuous decoloring method of L-valine fermentation liquid | |
| CN104232703A (en) | L-phenylalanine production process | |
| CN110129289B (en) | Production method of enzyme preparation in methanol protein fermentation broth | |
| CN112384630B (en) | Anti-viscosity method for catalytic production of phosphatidylserine enzyme and method for producing phosphatidylserine by using same | |
| NL2028015B1 (en) | Process method for producing erythritol from wheat starch |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170405 |
|
| WD01 | Invention patent application deemed withdrawn after publication |