CN104261947A - Fertilizer prepared by utilizing threonine fermented wastes - Google Patents
Fertilizer prepared by utilizing threonine fermented wastes Download PDFInfo
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- CN104261947A CN104261947A CN201410519206.5A CN201410519206A CN104261947A CN 104261947 A CN104261947 A CN 104261947A CN 201410519206 A CN201410519206 A CN 201410519206A CN 104261947 A CN104261947 A CN 104261947A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
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- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention belongs to the field of fermented waste liquid treatment in the biological fermentation industry and discloses a fertilizer prepared by utilizing threonine fermented wastes. The fertilizer is prepared by the following steps: (1) preparing threonine fermentation liquor; (2) filtering and discoloring; (3) separating crystals and a mother solution; (4) hydrolyzing mycoprotein; (5) preparing feed liquid A; and (6) preparing the fertilizer. According to the fertilizer, amino acid fermented wastes are effectively utilized, the production cost of the fertilizer is reduced, the wastes are changed into valuable materials, the fertilizer efficiency is lasting, and the fertilizer is superior to like products in the market.
Description
Technical field
The invention belongs to biological fermentation industry Wastewater treating field, be specifically related to a kind of fertilizer utilizing Threonine Fermentation waste to prepare.
Background technology
Threonine is a kind of essential amino acid, the second limiting amino acid of pig feed and the 3rd limiting amino acid of poultry feed, along with Methionin, the widespread use of methionine(Met) synthetics in mixed feed, Threonine becomes the major limiting factors affecting growth of animals or poultry gradually.Threonine is mainly used as fodder additives, and its production stage is concentrated, crystallization, fox extracting thallus protein, and the tropina produced in its process and mother liquid disposal difficulty are comparatively large, and cost is higher, is difficult to obtain the higher byproduct of sexual valence.
The development of China's fertilizer and application history, be main phase from farm manure to use inorganic fertilizer, because farm manure source of pollution are many, traffic capacity is large, operationally wastes time and energy and effect is not obvious especially; And inorganic fertilizer composition is single, utilization ratio is lower, and often causes soil compaction and adjoint soil erosion polluted source, influence ecological environment.At present, the inorganic composite fertilizer etc. of market many employings Nitrogen-and Phosphorus-containing potassium, the fertilizer efficiency of this composite fertilizer is comprehensive, but it is lasting also to there is fertilizer efficiency, the defects such as easy loss; The more important thing is, the price of current composite fertilizer is higher, brings larger burden to peasant, how to reduce fertilizer cost, and improving farmers' income is the agricultural modernization technical issues that need to address.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of fertilizer utilizing Threonine Fermentation waste to prepare, this fertilizer effectively make use of amino acid fermentation waste material, reduce fertiliser production cost, achieve and turn waste into wealth, and fertilizer efficiency is lasting, is better than market like product.
For achieving the above object, the present invention is by the following technical solutions:
Utilize a fertilizer prepared by Threonine Fermentation waste, it comprises the steps:
Step 1) prepares threonine fermentation liquid, step 2) to filter and decolouring, step 3) isolation of crystalline and mother liquor, step 4) hydrolysis tropina, step 5) prepares feed liquid A, and step 6) prepares fertilizer;
Particularly, be prepared from accordance with the following steps:
1) threonine fermentation liquid is prepared: colibacillus engineering K12 △ dapA and brevibacterium flavum ATCC14067 mixed bacteria liquid are cultivated according in the inoculum size access seeding tank of 10%, it is 32 DEG C in temperature, shaking speed is 180-200r/min, cultivates and obtains liquid A in 12-16 hour; Then according to liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 32 DEG C, cultivates 5 days, obtains fermented liquid;
2) filter and decolour: fermentation liquor mocromembrane filters, and described mocromembrane aperture is the centrifugal 3-5 minute of 0.04-0.06 μm, 3000r/min, collecting bacteria-removing liquid and tropina; Then bacteria-removing liquid is pumped into bleacher and carry out desolventing technology, add accounting for the gac of bacteria-removing liquid quality 1-1.5% in bleacher, the temperature controlled in bleacher is 45 DEG C, decolour after 30 points, obtains liquid B with Plate Filtration removal gac;
3) isolation of crystalline and mother liquor: liquid B is concentrated into 1/3 of stoste volume through evaporator, then crystallization, last collected by centrifugation Threonine crystal and mother liquor;
4) tropina is hydrolyzed: dried by tropina, pulverizer is ground into Powdered, then reactor is placed in, add the hydrochloric acid of 6-8mol/L, be as the criterion not have raw material, hydrolysis 12-15 hour is stirred at 60 DEG C of temperature, stirring velocity is 100 turns/min, use in potassium hydroxide after reaction terminating and remaining hydrochloric acid, obtain amino acid solution, then the ratio being 3:1 according to the mol ratio of amino acid and metal ion adds metal ion, control temperature is 40 DEG C, time is 30min, pH carries out chelatropic reaction 7.0, finally chelate products is concentrated, drying and pulverizing, obtain amino-acid chelate,
5) feed liquid A is prepared: add in stirred pot by amino-acid chelate, corn stalk powder, diatomite and mother liquor according to the mass ratio of 1:3:3:10,500 revs/min are stirred 10 minutes, then retort is entered, the temperature controlling retort is 100 DEG C, react 15 minutes, then be cooled to room temperature, obtain feed liquid A; Wherein, amino-acid chelate, corn stalk powder or diatomaceous particle diameter all control at 50-100 order;
6) fertilizer is prepared: according to the mixing of feed liquid A, monoammonium phosphate, urea and water, 1000 turns/min stirs 5min, then joins granulation in double-screw extruding pelletizing machine, dry packing and get final product; Wherein, the part by weight of feed liquid A, monoammonium phosphate, urea and water is 3:2:2:1.
Wherein, preferably, described mixed bacteria liquid is that 5: 1 mixing are obtained by colibacillus engineering liquid and brevibacterium flavum liquid according to volume ratio;
Described metal ion is formed by cupric ion, calcium ion and the zine ion mixed in molar ratio according to 1:1:1.
The beneficial effect that the present invention obtains mainly comprises:
The present invention amino acid whosely make use of tropina and mother liquor in production simultaneously, eliminates mother liquid disposal process, reduces production cost, achieves and turn waste into wealth, increased substantially the utilization ratio of resource; The present invention is prepared into chelating amino acid by utilizing tropina, avoids using expensive protein raw materials, and substantially increases fertilizer efficiency; Fertilizer prepared by the present invention not only employs fermentation fertilizer, also added wastes of crops, reduces raw materials cost, and fertilizer efficiency is better, is applicable to multiple kinds of crops; The present invention adds potassium hydroxide as neutralizing agent, and its sylvite produced can be used as composite fertilizer's potassium component; The present invention on the basis of existing technology, found by lot of experiments, two kinds of bacterium liquid are adopted to mix according to a certain percentage, therebetween certain synergy is possessed, than conventional single strain fermentation method, greatly can improve the output of Threonine, adopt best proportion 5: 1 to mix, under the prerequisite that other conditions are constant, output can improve more than 20% compared with the single bacterial strain of intestinal bacteria.
Embodiment
Technical scheme in the application is understood better in order to make those skilled in the art person, below in conjunction with the application's specific embodiment, carry out clearly, intactly describing to the present invention, obviously, described embodiment is only some embodiments of the present application, instead of whole embodiments.Based on the embodiment in the application, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, should belong to the scope of protection of the invention.
Embodiment 1
Utilize a fertilizer prepared by Threonine Fermentation waste, it comprises the steps:
Step 1) prepares threonine fermentation liquid, step 2) to filter and decolouring, step 3) isolation of crystalline and mother liquor, step 4) hydrolysis tropina, step 5) prepares feed liquid A, and step 6) prepares fertilizer;
1) threonine fermentation liquid is prepared: can see " colibacillus engineering K12 △ dapA ferments and produces the optimization of L-threonine substratum " by colibacillus engineering K12 △ dapA(, Agriculture in Hunan science) and brevibacterium flavum ATCC14067(can see documents such as CN1305009A and CN1284560A) mixed bacteria liquid cultivates according in the inoculum size access seeding tank of 10%, it is 32 DEG C in temperature, shaking speed is 180r/min, cultivates and obtains liquid A in 12 hours; Then according to liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 32 DEG C, cultivates 5 days, obtains fermented liquid; The basic parameter of obtained fermented liquid is: threonine content: 13g/100ml, dry: 15g/100ml; Wherein, by colibacillus engineering liquid, (concentration is 1 × 10 to mixed bacteria liquid
8individual/mL) and brevibacterium flavum liquid (concentration is 1 × 10
8individual/mL) be that 5: 1 mixing are obtained according to volume ratio; Seed tank culture base component is (1L): yeast extract paste 5g, glucose 3g, ammonium sulfate 0.5g, iron vitriol 0.02g, MgSO
40.02g, KH
2pO
40.1g, all the other are water, pH value 6.5; Described Fermenter Medium Component is: glucose 60g/L, corn steep liquor 20g/L, ammonium sulfate 2g/L, KH
2pO
40.1g/L, MgSO
42g/L, NaCl 0.2g/L, pH value 6.5;
2) filter and decolour: fermentation liquor mocromembrane filters, and described mocromembrane aperture is 0.04 μm, centrifugal 3 minutes of 3000r/min, collecting bacteria-removing liquid and tropina; Then bacteria-removing liquid is pumped into bleacher and carry out desolventing technology, add accounting for the gac of bacteria-removing liquid quality 1.5% in bleacher, the temperature controlled in bleacher is 45 DEG C, decolour after 30 points and obtains liquid B with Plate Filtration removal gac;
3) isolation of crystalline and mother liquor: liquid B is concentrated into 1/3 of stoste volume through evaporator, with the crystallization of intermittent type single-action condensing crystal pot, start to add crystal seed when feed liquid in pot is concentrated into degree Beaume 27, crystallisation process control temperature 65 DEG C, vacuum tightness 0.08MPa, last collected by centrifugation Threonine crystal and mother liquor;
4) tropina is hydrolyzed: dried by tropina, pulverizer is ground into Powdered, then reactor is placed in, add the hydrochloric acid of 8mol/L, be as the criterion not have raw material, hydrolysis is stirred 12 hours at 60 DEG C of temperature, stirring velocity is 100 turns/min, use in potassium hydroxide after reaction terminating and remaining hydrochloric acid, solution is made to be neutral, obtain amino acid solution, then the ratio being 3:1 according to the mol ratio of amino acid and metal ion adds metal ion, control temperature is 40 DEG C, time is 30min, pH carries out chelatropic reaction 7.0, finally chelate products is concentrated, drying and pulverizing, obtain amino-acid chelate, described metal ion is by cupric ion (Cu
+ 2), calcium ion (Ca
+ 2) and zine ion (Zn
+ 2) form according to the mixed in molar ratio of 1:1:1,
5) feed liquid A is prepared: add in stirred pot by amino-acid chelate, corn stalk powder, diatomite and mother liquor according to the mass ratio of 1:3:3:10,500 revs/min are stirred 10 minutes, then retort is entered, the temperature controlling retort is 100 DEG C, react 15 minutes, then be cooled to room temperature, obtain feed liquid A; Wherein, amino-acid chelate, corn stalk powder or diatomaceous particle diameter all control at 50 orders;
6) fertilizer is prepared: according to the mixing of feed liquid A, monoammonium phosphate, urea and water, 1000 turns/min stirs 5min, then joins granulation in double-screw extruding pelletizing machine, dry packing and get final product; Wherein, the part by weight of feed liquid A, monoammonium phosphate, urea and water is 3:2:2:1.
Embodiment 2
Utilize a fertilizer prepared by Threonine Fermentation waste, it comprises the steps:
Step 1) prepares threonine fermentation liquid, step 2) to filter and decolouring, step 3) isolation of crystalline and mother liquor, step 4) hydrolysis tropina, step 5) prepares feed liquid A, and step 6) prepares fertilizer;
1) threonine fermentation liquid is prepared: can see " colibacillus engineering K12 △ dapA ferments and produces the optimization of L-threonine substratum " by colibacillus engineering K12 △ dapA(, Agriculture in Hunan science) and brevibacterium flavum ATCC14067(can see documents such as CN1305009A and CN1284560A) mixed bacteria liquid cultivates according in the inoculum size access seeding tank of 5%, it is 32 DEG C in temperature, shaking speed is 200r/min, cultivates and obtains liquid A in 16 hours; Then according to liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 32 DEG C, cultivates 5 days, obtains fermented liquid; The basic parameter of obtained fermented liquid is: threonine content: 14g/100ml, dry: 16g/100ml; Wherein, by colibacillus engineering liquid, (concentration is 1 × 10 to mixed bacteria liquid
8individual/mL) and brevibacterium flavum liquid (concentration is 1 × 10
8individual/mL) be that 5: 1 mixing are obtained according to volume ratio; Seed tank culture base component is (1L): yeast extract paste 5g, glucose 3g, ammonium sulfate 0.5g, iron vitriol 0.02g, MgSO
40.02g, KH
2pO
40.1g, all the other are water, pH value 6.5; Described Fermenter Medium Component is: glucose 60g/L, corn steep liquor 20g/L, ammonium sulfate 2g/L, KH
2pO
40.1g/L, MgSO
42g/L, NaCl 0.2g/L, pH value 6.5;
2) filter and decolour: fermentation liquor mocromembrane filters, and described mocromembrane aperture is 0.06 μm, centrifugal 3 minutes of 3000r/min, collecting bacteria-removing liquid and tropina; Then bacteria-removing liquid is pumped into bleacher and carry out desolventing technology, add accounting for the gac of bacteria-removing liquid quality 1% in bleacher, the temperature controlled in bleacher is 45 DEG C, decolour after 30 points and obtains liquid B with Plate Filtration removal gac;
3) isolation of crystalline and mother liquor: liquid B is concentrated into 1/3 of stoste volume through evaporator, with the crystallization of intermittent type single-action condensing crystal pot, the spontaneous nucleation when feed liquid in pot is concentrated into degree Beaume 27, crystallisation process control temperature 65 DEG C, vacuum tightness 0.08MPa, last collected by centrifugation Threonine crystal and mother liquor;
4) tropina is hydrolyzed: dried by tropina, pulverizer is ground into Powdered, then reactor is placed in, add the hydrochloric acid of 6mol/L, be as the criterion not have raw material, hydrolysis is stirred 15 hours at 60 DEG C of temperature, stirring velocity is 200 turns/min, use in potassium hydroxide after reaction terminating and remaining hydrochloric acid, obtain amino acid solution, then the ratio being 3:1 according to the mol ratio of amino acid and metal ion adds metal ion, control temperature is 40 DEG C, time is 30min, pH carries out chelatropic reaction 7.5, finally chelate products is concentrated, drying and pulverizing, obtain amino-acid chelate, described metal ion is by cupric ion (Cu
+ 2), calcium ion (Ca
+ 2) and zine ion (Zn
+ 2) form according to the mixed in molar ratio of 1:1:1,
5) feed liquid A is prepared: add in stirred pot by amino-acid chelate, corn stalk powder, diatomite and mother liquor according to the mass ratio of 1:3:3:10,500 revs/min are stirred 10 minutes, then retort is entered, the temperature controlling retort is 100 DEG C, react 15 minutes, then be cooled to room temperature, obtain feed liquid A; Wherein, amino-acid chelate, corn stalk powder or diatomaceous particle diameter all control at 100 orders;
6) fertilizer is prepared: according to the mixing of feed liquid A, monoammonium phosphate, urea and water, 1000 turns/min stirs 5min, then joins granulation in double-screw extruding pelletizing machine, dry packing and get final product; Wherein, the part by weight of feed liquid A, monoammonium phosphate, urea and water is 3:2:2:1.
Embodiment 3
The manure trial of fertilizer of the present invention
One, the effect of plantation Chinese sorghum is fat tests:
Control group adopts composite fertilizer (nitrogen-phosphorus-potassium=23-7-6); The fertilizer that experimental group adopts the embodiment of the present invention 1 to prepare; The soil property in experimental plot is identical with planting conditions, and area is one mu; Rate of fertilizer application is 80kg/ mu.Concrete test-results is in table 1:
Table 1
| Group | Per mu yield (Kg) | Volume increase (%) | Fertilising drops into (unit) |
| Control group | 487.9 | / | 208 |
| Experimental group | 523.1 | 7.21 | 168 |
Note: control group fertilizer calculates according to 2.6 yuan/kg, experimental group calculates according to 2.1 yuan/kg.
Two, the experiment of Ipomoea batatas fertilizer efficiency is planted:
Arrange two process experimental plots, area is one mu, is control group and experimental group.Control group: Nitrogen, Phosphorus and Potassium (N16-P22-K7); Experimental group: fertilizer prepared by embodiment 1; The equal mu fertilising in each experimental plot 60Kg, on May 17th, 2013 plants, and October 20 dug to obtain gather in then.Concrete test-results is in table 2:
Table 2
| ? | Per mu yield (Kg) | Per mu yield increment (Kg) | Mu fertilising drops into (unit) |
| Control group | 2456 | / | 162 |
| Experimental group | 2673 | 217 | 126 |
Remarks: control group fertilizer calculates according to price 2.7 yuan/kg, experimental group calculates according to 2.1 yuan/kg.
Three, rice cultivation manure trial:
Arrange two process experimental plots, area is one mu, is control group and experimental group.Control group: composite fertilizer (N16-P22-K7); Experimental group: fertilizer prepared by embodiment 2; The usage quantity often organizing experimental plot fertilizer is 50kg, and planting conditions is identical, gathers in the crops paddy rice simultaneously, measures paddy rice per mu yield and stimulation ratio; Detect paddy rice grain number per spike, thousand seed weight and unfilled gtains number simultaneously.Test-results is in table 3:
Table 3
| Group | Unfilled gtains number (grain/fringe) | Grain number per spike (grain/fringe) | Thousand seed weight (g) | Per mu yield (kg) |
| Control group | 5.3 | 64.5 | 28.7 | 471.9 |
| Experimental group | 3.2 | 67.8 | 30.1 | 489.2 |
Conclusion (of pressure testing): found by the test of plantation multiple kinds of crops, fertilizer prepared by the present invention commonly uses composite fertilizer's obvious effect of increasing production than market, with low cost, is conducive to the income improving plantation food crop, improves the enthusiasm of peasant; This fertilizer is applicable to the field crops such as longer jowar in vegetative period, Ipomoea batatas and paddy rice, and its effect of increasing production is comparatively remarkable, can use by further genralrlization.
Although above done detailed explanation with general explanation and embodiment to this case, on basis of the present invention, can make some modifications or improvements it, this will be apparent to those skilled in the art.Therefore, amendment done without departing from theon the basis of the spirit of the present invention or improvement, all belong to the scope of protection of present invention.
Claims (5)
1. the fertilizer utilizing Threonine Fermentation waste to prepare, is characterized in that, described fertilizer is prepared from accordance with the following steps:
Step 1) prepares threonine fermentation liquid, step 2) filter and decolouring, step 3) isolation of crystalline and mother liquor, step 4) hydrolysis tropina, step 5) prepares feed liquid A, and step 6) prepares fertilizer.
2. fertilizer according to claim 1, is characterized in that, described fertilizer is prepared from accordance with the following steps:
1) threonine fermentation liquid is prepared: cultivated according in the inoculum size access seeding tank of 10% by the mixed bacteria liquid of colibacillus engineering K12 △ dapA and brevibacterium flavum ATCC14067, it is 32 DEG C in temperature, shaking speed is 180-200r/min, cultivates and obtains liquid A in 12-16 hour; Then according to liquid A: fermentation tank culture medium be 1: 10 volume ratio proceed in fermentor tank and cultivate, temperature 32 DEG C, cultivates 5 days, obtains fermented liquid;
2) filter and decolour: fermentation liquor mocromembrane filters, and described mocromembrane aperture is the centrifugal 3-5 minute of 0.04-0.06 μm, 3000r/min, collecting bacteria-removing liquid and tropina; Then bacteria-removing liquid is pumped into bleacher and carry out desolventing technology, add accounting for the gac of bacteria-removing liquid quality 1-1.5% in bleacher, the temperature controlled in bleacher is 45 DEG C, and decolour after 30 points, removal gac obtains liquid B;
3) isolation of crystalline and mother liquor: liquid B is concentrated into 1/3 of stoste volume through evaporator, then crystallization, last collected by centrifugation Threonine crystal and mother liquor;
4) tropina is hydrolyzed: dried by tropina, pulverizer is ground into Powdered, then reactor is placed in, add the hydrochloric acid of 6-8mol/L, be as the criterion not have raw material, hydrolysis 12-15 hour is stirred at 60 DEG C of temperature, stirring velocity is 100 turns/min, use in potassium hydroxide after reaction terminating and remaining hydrochloric acid, obtain amino acid solution, then the ratio being 3:1 according to the mol ratio of amino acid and metal ion adds metal ion, control temperature is 40 DEG C, time is 30min, pH is 7.0, carry out chelatropic reaction, finally chelate products is concentrated, drying and pulverizing, obtain amino-acid chelate,
5) feed liquid A is prepared: add in stirred pot by amino-acid chelate, corn stalk powder, diatomite and mother liquor according to the mass ratio of 1:3:3:10,500 revs/min are stirred 10 minutes, then retort is entered, the temperature controlling retort is 100 DEG C, react 15 minutes, then be cooled to room temperature, obtain feed liquid A; Wherein, amino-acid chelate, corn stalk powder or diatomaceous particle diameter all control at 50-100 order;
6) fertilizer is prepared: according to the mixing of feed liquid A, monoammonium phosphate, urea and water, 1000 turns/min stirs 5min, then joins granulation in double-screw extruding pelletizing machine, dry packing and get final product; Wherein, the part by weight of feed liquid A, monoammonium phosphate, urea and water is 3:2:2:1.
3. let alone the fertilizer described in one according to claim 1-2, it is characterized in that, described mixed bacteria liquid mixes obtained by colibacillus engineering liquid and brevibacterium flavum liquid according to volume ratio 5: 1.
4. let alone the fertilizer described in one according to claim 1-3, it is characterized in that, described metal ion is formed by cupric ion, calcium ion and the zine ion mixed in molar ratio according to 1:1:1.
5. let alone the fertilizer described in one according to claim 1-4, it is characterized in that, described seed tank culture base is: yeast extract paste 5g, glucose 3g, ammonium sulfate 0.5g, iron vitriol 0.02g, MgSO
40.02g, KH
2pO
40.1g, all the other are water, configuration 1L, pH value 6.5; Described fermentation tank culture medium is: glucose 60g/L, corn steep liquor 20g/L, ammonium sulfate 2g/L, KH
2pO
40.1g/L, MgSO
42g/L, NaCl 0.2g/L, pH value 6.5.
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