CN105967889A - Soil conditioner containing glutamic acid fermentation mother liquor - Google Patents
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
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- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention belongs to the field of soil conditioners and discloses a soil conditioner containing glutamic acid fermentation mother liquor. The soil conditioner is prepared according to the following steps of 1, collection of the mother liquor and mycoprotein; 2, hydrolysis and neutralization; 3, preparation of a biological nitrogen fixation preparation; 4, preparation of a biological weeding preparation; 5, preparation of composite minerals; 6, preparation of the soil conditioner. The soil conditioner achieves nitrogen fixation and potassium maintenance effects in soil, soil is improved, and soil quality is improved.
Description
Technical field
The invention belongs to soil conditioner field, be specifically related to a kind of soil conditioner comprising glutamic acid fermentation mother solution.
Background technology
Glutamic acid, is a kind of acidic amino acid.Intramolecular contains two carboxyls, and chemical name is alpha-amido 1,3-propanedicarboxylic acid.Glutamic acid is that inner Suo Xun finds for 1856, and for clear crystal, having delicate flavour, be present in a large number in grain protein, in animal brain, content is the most more, can be used to produce monosodium glutamate.Account for critical role during glutamic acid protein metabolism in vivo, participate in the important chemical reaction of many in animal, plant and microorganism.Sodium glutamate is commonly called as monosodium glutamate, is important tasty agents, fragrance is had potentiation.
Glutamic acid fermentation mother solution have that CODCr is high, BOD5 high, thalline content is high, ammonia-nitrogen content is high and pH value (1.5-3.2) low " etc. feature.It it is the industrial wastewater that a kind of difficulty of governance is the biggest.Owing to can not effectively administer gourmet powder waste water, many Gourmet Powder Factories are put into the row of major polluting sources, whole nation unit, and the improvement of gourmet powder waste water has become as the great difficult problem of restriction glutamate production enterprise development.
In recent years, production and the amount of application of various chemical fertilizer are continuously increased, and the development to agricultural production serves important impetus.But it is as being continuously increased of applying quantity of chemical fertilizer, it is used shortcoming and the most gradually comes out, analyze to get up to be mainly manifested in the following aspects: fertilizer efficiency utilization rate is low, cause soil compaction serious, deteriorate the physicochemical property of soil, blocking soil aperture, destroys soil aggregate, causes that soil nutrient elements is unbalance, hardens, pollutes and fertility decline.For above-mentioned drawback, cultivation of fields is sought after one, energy activating soil, reduces the usage amount of chemical fertilizer, reduce the pollution to environment, and the soil conditioner to increasing crop yield.Conventional compound fertilizer is coordinated to use, with the shortcoming overcoming compound fertilizer with soil conditioner.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of soil conditioner comprising glutamic acid fermentation mother solution, this soil conditioner energy activating soil, reduces the usage amount of chemical fertilizer, has weeding function concurrently.
For achieving the above object, the present invention is by the following technical solutions:
A kind of soil conditioner comprising glutamic acid fermentation mother solution, it obtains in accordance with the following steps:
1) collect mother solution and tropina: glutami acid fermentation liquor filters, collect tropina and filtered solution respectively；Then filtered solution is used for preparing glutamic acid, collects the mother solution produced；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological nitrogen fixation preparation is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the mother solution of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind azotobacterin, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological nitrogen fixation preparation；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 10-15:8-12:5-7:3-5；
Described azotobacterin is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 4:3:3:2, to obtain final product；
4) biological weed control preparation is prepared: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, add bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, it is heated to 100 DEG C again with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation herbicidal formulations, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological weed control preparation；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 25-35:150-200:20-25:8-12:4-6:2-3；
Described herbicidal formulations is prepared according to following technique: trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:3:3, to obtain final product；
5) prepare composite mineral matter: by potassium humate, peat soil, bentonite and attapulgite according to the quality of 3:2:1:1 than mixing and stirring, then grind, cross 200 mesh sieves, to obtain final product；
6) soil conditioner is prepared: composite mineral matter, biological nitrogen fixation preparation, biological weed control preparation are mixed according to the mass ratio of 2-3:8-12:3-5, stir, then lyophilization, to obtain final product.
Preferably, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC
No.3770, described azotobacter chroococcum is ATCC No.4412, and bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Pseudomonas aeruginosa is CGMCC No.5721；Described candidiasis is ATCC No.22023.
Above-mentioned bacterial strains all can be bought from preservation center or other commercial channel and obtain.
The beneficial effect that the present invention obtains includes the following aspects:
Glutamic acid fermentation mother solution and tropina are applied all in soil conditioner preparation by the present invention, it is to avoid be evaporated waste liquid concentrating and subtract dirty process, decrease energy waste, improve utilization rate, and be prepared for soil conditioner, kill two birds with one stone；The tropina that direct hydrolysis of the present invention is discarded is as fermentation raw material, it is provided that abundant ammonium chloride and amino acid nitrogen source, can not only be used for fermentable nutriment, it is possible to use as the fertilizer efficiency part in soil conditioner；Bacterial strain compatibility is reasonable, uses different microbial inoculum different fermentations processing mode so that different microbial inoculums are in optimal fermentation condition, it is to avoid part bacterial strain can not be survived in culture fluid prepared by waste water；The present invention is by effectively utilizing the agricultural waste material of naturally occurring, in conjunction with microbial technique so that expect with low cost, regulates soil granular, maintains element balance, the molten potassium of fixed nitrogen；Composite mineral matter can improve soil, and discharges multiple middle and trace element in soil, increases soil fertility, improves soil compaction, moreover it is possible to use as microbe carrier, kills two birds with one stone；Microbial inoculum of the present invention is through screening and reasonable compatibility so that regulator possesses fixed nitrogen water conservation, and increasing crop yield increases income, and also has both certain weeding function, environment friendly and pollution-free.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of soil conditioner comprising glutamic acid fermentation mother solution, it obtains in accordance with the following steps:
1) collect mother solution and tropina: glutami acid fermentation liquor filters, collect tropina and filtered solution respectively；Then filtered solution is used for preparing glutamic acid, collects the mother solution produced；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological nitrogen fixation preparation is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the mother solution of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind azotobacterin, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological nitrogen fixation preparation；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 10:8:5:3；
Described azotobacterin is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 4:3:3:2, to obtain final product；
4) biological weed control preparation is prepared: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, add bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, it is heated to 100 DEG C again with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation herbicidal formulations, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological weed control preparation；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 25:150:20:8:4:2；
Described herbicidal formulations is prepared according to following technique: trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:3:3, to obtain final product；
5) prepare composite mineral matter: by potassium humate, peat soil, bentonite and attapulgite according to the quality of 3:2:1:1 than mixing and stirring, then grind, cross 200 mesh sieves, to obtain final product；
6) soil conditioner is prepared: composite mineral matter, biological nitrogen fixation preparation, biological weed control preparation are mixed according to the mass ratio of 2:8:3, stir, then lyophilization, to obtain final product.
Wherein, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC No.3770, and described azotobacter chroococcum is ATCC
No.4412, bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；Described candidiasis is ATCC No.22023.
Embodiment 2
A kind of soil conditioner comprising glutamic acid fermentation mother solution, it obtains in accordance with the following steps:
1) collect mother solution and tropina: glutami acid fermentation liquor filters, collect tropina and filtered solution respectively；Then filtered solution is used for preparing glutamic acid, collects the mother solution produced；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological nitrogen fixation preparation is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the mother solution of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind azotobacterin, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological nitrogen fixation preparation；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 15:12:7:5；
Described azotobacterin is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 4:3:3:2, to obtain final product；
4) biological weed control preparation is prepared: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, add bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, it is heated to 100 DEG C again with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation herbicidal formulations, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological weed control preparation；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 35:200:25:12:6:3；
Described herbicidal formulations is prepared according to following technique: trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:3:3, to obtain final product；
5) prepare composite mineral matter: by potassium humate, peat soil, bentonite and attapulgite according to the quality of 3:2:1:1 than mixing and stirring, then grind, cross 200 mesh sieves, to obtain final product；
6) soil conditioner is prepared: composite mineral matter, biological nitrogen fixation preparation, biological weed control preparation are mixed according to the mass ratio of 3:12:5, stir, then lyophilization, to obtain final product.
Wherein, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC No.3770, and described azotobacter chroococcum is ATCC
No.4412, bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC No.32248；Pseudomonas aeruginosa is CGMCC No.5721；Described candidiasis is ATCC No.22023.
Embodiment 3
The field test effect of the soil conditioner of the embodiment of the present invention 2 preparation: as a example by Semen Tritici aestivi.
Matched group: Common compound fertilizer (N-P-K content is 15: 15: 17)；
Test group: the soil conditioner of Common compound fertilizer (N-P-K content is 15: 15: 17)+embodiment 2 preparation.
Experimental technique: select experimental plot Winter Wheat Planted, the equal 10 mu of ground of experimental plot area that two groups of fertilizer processes respectively.
Matched group: Common compound fertilizer usage amount is 40kg, test group: Common compound fertilizer usage amount is the Chemical Mixed Fertilizer of 30kg+10kg embodiment 2 preparation；Other planting conditions are identical；Gather in the crops Semen Tritici aestivi simultaneously, measure Semen Tritici aestivi per mu yield and rate of growth；Detect wheat grains per spike, mass of 1000 kernel simultaneously.Experimental result: with reference to table 1.
Table 1
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Matched group | 26.8 | 47.1 | 362 |
| Test group | 30.7 | 48.8 | 393 |
Same treatment conditions, Second Year multiple cropping Semen Tritici aestivi, experimental result: with reference to table 2.
Table 2
| Group | Grain number per spike | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Matched group | 26.2 | 46.3 | 351 |
| Test group | 30.9 | 49.1 | 402 |
Conclusion: compare discovery by table 1-2, test group wheat grains per spike, mass of 1000 kernel and per mu yield, apparently higher than matched group, possess statistical significance；And the effect of high-yield of Second Year is more preferably, illustrates that soil conditioner serves fixed nitrogen in soil and protects the effects such as potassium, improve soil quality, it is achieved that sustainable development.
Herbicidal effect: test group is compared with matched group, after applying fertilizer 1 month: the quantity of the weeds such as Herba chenopodii, Herba Portulacae, barnyard grass, Herba Acalyphae significantly reduces, and wherein, Herba Portulacae all decreases 50%, and barnyard grass decreases 60%；Herbicidal effect is good, can replace or reduce the use of herbicide, and environmentally safe.
The detection of soil and fertilizer: test group is compared with matched group, and the content of available phosphorus improves 22.1%, and the content of available potassium improves 31.5%.
Present disclosure is not limited to cited by embodiment, and the conversion of any equivalence that technical solution of the present invention is taked by those of ordinary skill in the art by reading description of the invention, the claim being the present invention is contained.
Claims (4)
1. comprising a soil conditioner for glutamic acid fermentation mother solution, it is prepared in accordance with the following steps: 1) collection mother solution and tropina, and 2) hydrolyze and neutralize, 3) biological nitrogen fixation preparation is prepared, 4) biological weed control preparation is prepared, 5) prepare composite mineral matter, 6) prepare soil conditioner.
Soil conditioner the most according to claim 1, it is characterised in that described soil conditioner is prepared in accordance with the following steps:
1) collect mother solution and tropina: glutami acid fermentation liquor filters, collect tropina and filtered solution respectively；Then filtered solution is used for preparing glutamic acid, collects the mother solution produced；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological nitrogen fixation preparation is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the mother solution of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind azotobacterin, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological nitrogen fixation preparation；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 10-15:8-12:5-7:3-5；
4) biological weed control preparation is prepared: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, add bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, it is heated to 100 DEG C again with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation herbicidal formulations, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological weed control preparation；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 25-35:150-200:20-25:8-12:4-6:2-3；
5) prepare composite mineral matter: by potassium humate, peat soil, bentonite and attapulgite according to the quality of 3:2:1:1 than mixing and stirring, then grind, cross 200 mesh sieves, to obtain final product；
6) soil conditioner is prepared: composite mineral matter, biological nitrogen fixation preparation, biological weed control preparation are mixed according to the mass ratio of 2-3:8-12:3-5, stir, then lyophilization, to obtain final product.
Soil conditioner the most according to claim 2, it is characterised in that
Described azotobacterin is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 4:3:3:2, to obtain final product.
Soil conditioner the most according to claim 2, it is characterised in that described herbicidal formulations is prepared according to following technique: trichoderma aureoviride, Pseudomonas aeruginosa, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 5:3:3, to obtain final product.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107522572A (en) * | 2017-08-08 | 2017-12-29 | 河南五吉元肥业有限公司 | A kind of organic fertilizer and preparation method thereof |
| CN110643526A (en) * | 2019-08-31 | 2020-01-03 | 暨南大学 | Method for promoting colonization of azotobacter chroococcum in plant rhizosphere |
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| CN1413963A (en) * | 2002-09-19 | 2003-04-30 | 上海九康农业科技发展有限公司 | Compound application technology for nano-biological fertilizer and soil conditioner |
| CN104046363A (en) * | 2013-03-15 | 2014-09-17 | 上海微明生物科技有限公司 | Method for preparing soil restoration modifier by utilization of spore fermentation wastewater |
| CN104261947A (en) * | 2014-10-07 | 2015-01-07 | 内蒙古阜丰生物科技有限公司 | Fertilizer prepared by utilizing threonine fermented wastes |
| CN104591928A (en) * | 2015-02-04 | 2015-05-06 | 周一鸿 | Bio-organic fertilizer with soil remediation function |
| CN104725147A (en) * | 2015-02-12 | 2015-06-24 | 新疆阜丰生物科技有限公司 | Process for preparing organic-inorganic compound fertilizer from bio-fermented amino acid wastewater |
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| CN107522572A (en) * | 2017-08-08 | 2017-12-29 | 河南五吉元肥业有限公司 | A kind of organic fertilizer and preparation method thereof |
| CN110643526A (en) * | 2019-08-31 | 2020-01-03 | 暨南大学 | Method for promoting colonization of azotobacter chroococcum in plant rhizosphere |
| CN110643526B (en) * | 2019-08-31 | 2022-06-14 | 暨南大学 | Method for promoting colonization of azotobacter chroococcum in plant rhizosphere |
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