CN106010561A - Soil conditioning agent containing threonine fermentation waste - Google Patents
Soil conditioning agent containing threonine fermentation waste Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K17/00—Soil-conditioning materials or soil-stabilising materials
- C09K17/40—Soil-conditioning materials or soil-stabilising materials containing mixtures of inorganic and organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
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Abstract
The invention discloses a soil conditioning agent containing threonine fermentation waste, which belongs to the field of soil conditioning agents. The soil conditioning agent is prepared through the following steps: 1) collection of mycoprotein and wastewater; 2) hydrolysis and neutralization; 3) preparation of a biological preparation A; 4) preparation of a biological preparation B; 5) preparation of a biological preparation C; and 6) preparation of the soil conditioning agent. The soil conditioning agent can realize yield increase of crops like paddy rice and sweet potatoes and improves soil quality.
Description
Technical field
The invention belongs to soil conditioner field, be specifically related to a kind of soil conditioner containing Threonine Fermentation garbage.
Background technology
Threonine is a kind of essential amino acid, it is the second limiting amino acid and the 3rd limiting amino acid of poultry feed of pig feed, along with the extensive application in mixed feed of lysine, methionine composite, threonine is increasingly becoming the major limiting factors affecting growth of animals or poultry.Threonine is mainly used as feed additive, and its production stage is concentration, crystallization, fox extracting thallus protein, and the tropina produced during it and mother liquid disposal difficulty are relatively big, relatively costly, it is difficult to the side-product that acquisition sexual valence is higher.
In recent years, production and the amount of application of various chemical fertilizer are continuously increased, and the development to agricultural production serves important impetus.But it is as being continuously increased of applying quantity of chemical fertilizer, it is used shortcoming and the most gradually comes out, analyze to get up to be mainly manifested in the following aspects: fertilizer efficiency utilization rate is low, cause soil compaction serious, deteriorate the physicochemical property of soil, blocking soil aperture, destroys soil aggregate, causes that soil nutrient elements is unbalance, hardens, pollutes and fertility decline.For above-mentioned drawback, cultivation of fields is sought after one, energy activating soil, reduces the usage amount of chemical fertilizer, reduce the pollution to environment, and the soil conditioner to increasing crop yield.Conventional compound fertilizer is coordinated to use, with the shortcoming overcoming chemical fertilizer with soil conditioner.
Summary of the invention
In order to overcome the defect of prior art, the invention provides a kind of soil conditioner containing Threonine Fermentation garbage, this soil conditioner energy activating soil, reduces the usage amount of chemical fertilizer, has weeding function concurrently.
For achieving the above object, the present invention is by the following technical solutions:
A kind of soil conditioner containing Threonine Fermentation garbage, it comprises the steps:
1) tropina and waste water are collected: the Threonine Fermentation garbage produced by fermentable threonine filters, and collects tropina and waste water；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological preparation A is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological preparation A；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 20-30:15-20:8-12:5-7；
Described microbial inoculum A is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:2:2:1, to obtain final product；
4) prepare biological preparation B: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, adding bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, then are heated to 100 DEG C with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation microbial inoculum B, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological preparation B；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 17-22:120-150:12-15:7-10:4-5:2-3；
Described microbial inoculum B is prepared according to following technique: trichoderma aureoviride, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 1:1, to obtain final product；
5) adjuvant C is prepared: by potassium humate, peat soil and attapulgite according to the quality of 2:2:1 than mixing and stirring, then grind, cross 200 mesh sieves, obtain adjuvant C；
6) soil conditioner is prepared: biological preparation A, biological preparation B and adjuvant C are mixed according to the mass ratio of 12-20:7-9:3-4, stir, then lyophilization, to obtain final product.
Preferably, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC
No.3770, described azotobacter chroococcum is ATCC No.4412, and described bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Above-mentioned bacterial strains all can be bought from preservation center or other commercial channel and obtain.
The beneficial effect that the present invention obtains includes the following aspects:
Threonine Fermentation garbage is applied all in soil conditioner preparation by the present invention, it is to avoid is evaporated waste liquid concentrating and subtract dirty process, decreases energy waste, improve utilization rate, and be prepared for soil conditioner, kill two birds with one stone；The tropina that direct hydrolysis of the present invention is discarded is as fermentation raw material, it is provided that abundant ammonium chloride and amino acid nitrogen source, can not only be used for fermentable nutriment, it is possible to use as the fertilizer efficiency part in soil conditioner；Bacterial strain compatibility is reasonable, uses different microbial inoculum different fermentations processing mode so that different microbial inoculums are in optimal fermentation condition, it is to avoid part bacterial strain can not be survived in culture fluid prepared by waste water；The present invention is by effectively utilizing the agricultural waste material of naturally occurring, in conjunction with microbial technique so that expect with low cost, regulates soil granular, maintains element balance, the molten potassium of fixed nitrogen；Composite mineral matter can improve soil, and discharges multiple middle and trace element in soil, increases soil fertility, improves soil compaction, moreover it is possible to use as microbe carrier, kills two birds with one stone；Microbial inoculum of the present invention is through screening and reasonable compatibility so that regulator possesses fixed nitrogen water conservation, and increasing crop yield increases income, and also has both certain weeding function, environment friendly and pollution-free.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and be easy to understand with effect, below in conjunction with specific embodiment, the present invention is expanded on further.
Embodiment 1
A kind of soil conditioner containing Threonine Fermentation garbage, is prepared according to following technique:
1) tropina and waste water are collected: the Threonine Fermentation garbage produced by fermentable threonine filters, and collects tropina and waste water；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological preparation A is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological preparation A；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 20:15:8:5；
Described microbial inoculum A is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:2:2:1, to obtain final product；
4) prepare biological preparation B: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, adding bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, then are heated to 100 DEG C with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation microbial inoculum B, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological preparation B；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 17:120:12:7:4:2；
Described microbial inoculum B is prepared according to following technique: trichoderma aureoviride, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 1:1, to obtain final product；
5) adjuvant C is prepared: by potassium humate, peat soil and attapulgite according to the quality of 2:2:1 than mixing and stirring, then grind, cross 200 mesh sieves, obtain adjuvant C；
6) soil conditioner is prepared: biological preparation A, biological preparation B and adjuvant C are mixed according to the mass ratio of 12:7:3, stir, then lyophilization, to obtain final product.
Wherein, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC
No.3770, described azotobacter chroococcum is ATCC No.4412, and described bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Embodiment 2
A kind of soil conditioner containing Threonine Fermentation garbage, is prepared according to following technique:
1) tropina and waste water are collected: the Threonine Fermentation garbage produced by fermentable threonine filters, and collects tropina and waste water；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological preparation A is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological preparation A；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 30:20:12:7；
Described microbial inoculum A is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:2:2:1, to obtain final product；
4) prepare biological preparation B: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, adding bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, then are heated to 100 DEG C with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation microbial inoculum B, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological preparation B；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 22:150:15:10:5:3；
Described microbial inoculum B is prepared according to following technique: trichoderma aureoviride, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 1:1, to obtain final product；
5) adjuvant C is prepared: by potassium humate, peat soil and attapulgite according to the quality of 2:2:1 than mixing and stirring, then grind, cross 200 mesh sieves, obtain adjuvant C；
6) soil conditioner is prepared: biological preparation A, biological preparation B and adjuvant C are mixed according to the mass ratio of 20:9:4, stir, then lyophilization, to obtain final product.
Wherein, described arthrobacterium is CGMCC No.7779, and described bacillus megaterium is CGMCC
No.3770, described azotobacter chroococcum is ATCC No.4412, and described bacillus polymyxa is CGMCC No.1325, and described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC No.22023.
Embodiment 3
The field test effect of the soil conditioner of the embodiment of the present invention 2 preparation: as a example by Oryza sativa L..
Matched group: Common compound fertilizer (N15P15K17)；
Test group: the soil conditioner of Common compound fertilizer (N15P15K17)+embodiment 2 preparation.
Experimental technique: select experimental plot rice cultivation, the equal 10 mu of ground of experimental plot area that two groups of fertilizer processes respectively.
Matched group: Common compound fertilizer usage amount is 60kg, test group: Common compound fertilizer usage amount is the soil conditioner of 50kg+10kg embodiment 2 preparation；Planting conditions is identical, gathers in the crops Oryza sativa L. simultaneously, measures Oryza sativa L. per mu yield, Oryza sativa L. grain number per spike and mass of 1000 kernel.Experimental result: see table 1
Table 1
| Group | Grain number per spike (grain/fringe) | Mass of 1000 kernel (g) | Per mu yield (kg) |
| Matched group | 64.9 | 28.46 | 479.5 |
| Test group | 68.8 | 32.18 | 528.3 |
Conclusion: compare discovery by table 1, test group grain number per spike, mass of 1000 kernel and per mu yield, apparently higher than matched group, possess statistical significance.
Second Year plants Radix Ipomoeae at rice stubble, and fertilizer efficiency experiment is as follows: matched group uses compound fertilizer (N16-P16-K16) 60kg/ mu, and experimental group uses the soil conditioner 10g/ mu of compound fertilizer (N16-P16-K16) 50kg/ mu+embodiment 2.Soil property is identical with planting conditions, and area is 10 mu.Experimental group per mu yield is 2734Kg, and matched group per mu yield is 2296Kg, per mu increases production 20%.
Herbicidal effect: rice test group is compared with matched group, after applying fertilizer 1 month: the quantity of the weeds such as barnyard grass, Herba Digitariae, Herba Acalyphae significantly reduces, and wherein, barnyard grass and Herba Digitariae all decrease 60%, and Herba Acalyphae decreases 65%；Herbicidal effect is good, can replace or reduce the use of herbicide, and environmentally safe.
The detection of soil and fertilizer: rice test group is compared with matched group, and the content of available phosphorus improves 25.3%, and the content of available potassium improves 33.6%.
Present disclosure is not limited to cited by embodiment, and the conversion of any equivalence that technical solution of the present invention is taked by those of ordinary skill in the art by reading description of the invention, the claim being the present invention is contained.
Claims (4)
1. containing a soil conditioner for Threonine Fermentation garbage, it is prepared according to following technique: 1) collect tropina and waste water, and 2) hydrolyze and neutralize, 3) biological preparation A is prepared, 4) adjuvant C prepare biological preparation B, 5) is prepared, 6) prepare soil conditioner.
Soil conditioner the most according to claim 1, it is characterized in that, described soil conditioner is prepared according to following technique: 1) collect tropina and waste water: the Threonine Fermentation garbage produced by fermentable threonine filters, and collects tropina and waste water；
2) hydrolyze and neutralize: tropina is dried, pulverizer is ground into powder, it is subsequently placed in retort, add the hydrochloric acid of 5mol/L, not have raw material to be as the criterion, stirring hydrolysis 24 hours at a temperature of 60 DEG C, mixing speed is 300 turns/min, using in ammonia after reaction terminating and remaining hydrochloric acid, the pH controlling solution is 7.0-7.2；
3) biological preparation A is prepared: pulverized by Pericarppium arachidis hypogaeae and obtain peanut hull meal, then compound is obtained with Testa Tritici, rapeseed meal and fishbone powder mixing and stirring, add the waste water of the step 1) accounting for compound twice quality, soak 12 hours, boil 15min again, be cooled to room temperature, according to 10%(v/v) inoculum concentration kind microbial inoculum A, ferment 36 hours in the condition that temperature is 28-30 DEG C, prepare biological preparation A；Wherein, the mass ratio of peanut hull meal, Testa Tritici, rapeseed meal and fishbone powder is 20-30:15-20:8-12:5-7；
Described microbial inoculum A is prepared according to following technique: arthrobacterium, bacillus megaterium, azotobacter chroococcum, bacillus polymyxa are cultivated respectively to concentration be 1 × 108The bacterium solution of individual/ml, then according to the volume ratio mixing of 3:2:2:1, to obtain final product；
4) prepare biological preparation B: peanut hull meal is broken into peanut hull meal, add step 2 to) solution in, stir, stand 12 hours, adding bean cake, distiller grains, conch meal and glucose the most successively, 500 revs/min are stirred 3 minutes, then are heated to 100 DEG C with the programming rate of 5 DEG C/min, then 30min it is incubated, naturally cool to room temperature, according to 10%(v/v) inoculum concentration inoculation microbial inoculum B, control at pH6.8-7.2, temperature is that the condition of 28-30 DEG C is fermented 36 hours, obtains biological preparation B；Wherein, peanut hull meal, step 2) the mass ratio of solution, bean cake, distiller grains, conch meal and glucose be 17-22:120-150:12-15:7-10:4-5:2-3；
Described microbial inoculum B is prepared according to following technique: trichoderma aureoviride, candidiasis are cultivated respectively to concentration be 1 × 107The bacterium solution of individual/ml, then according to the volume ratio mixing of 1:1, to obtain final product；
5) adjuvant C is prepared: by potassium humate, peat soil and attapulgite according to the quality of 2:2:1 than mixing and stirring, then grind, cross 200 mesh sieves, obtain adjuvant C；
6) soil conditioner is prepared: biological preparation A, biological preparation B and adjuvant C are mixed according to the mass ratio of 12-20:7-9:3-4, stir, then lyophilization, to obtain final product.
Soil conditioner the most according to claim 2, it is characterised in that preferably, described arthrobacterium is CGMCC
No.7779, described bacillus megaterium is CGMCC
No.3770, described azotobacter chroococcum is ATCC
No.4412, described bacillus polymyxa is CGMCC
No.1325, described trichoderma aureoviride is ACCC
No.32248；Described candidiasis is ATCC
No.22023。
4. claim 1-3 is allowed to the application in proportion of crop planting of the soil conditioner described in.
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