CN110950702A - Comprehensive treatment process of threonine fermentation waste - Google Patents
Comprehensive treatment process of threonine fermentation waste Download PDFInfo
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- CN110950702A CN110950702A CN201911331366.6A CN201911331366A CN110950702A CN 110950702 A CN110950702 A CN 110950702A CN 201911331366 A CN201911331366 A CN 201911331366A CN 110950702 A CN110950702 A CN 110950702A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention belongs to the technical field of treatment of threonine fermentation wastes, and discloses a comprehensive treatment process of threonine fermentation wastes, which comprises the following steps: step 1) hydrolyzing mycoprotein; step 2), treating fermentation wastewater; step 3) preparing biocontrol bacterium liquid; and 4) preparing a fertilizer additive. The invention not only comprehensively treats the fermentation waste, but also prepares the additive used by the crops by utilizing the waste, thereby achieving two purposes.
Description
Technical Field
The invention belongs to the technical field of treatment of threonine fermentation wastes, and particularly relates to a comprehensive treatment process of threonine fermentation wastes.
Background
The threonine industry in China is developed later, basically no production is produced in China before the 90 s of the last century, and the production is started after the 90 s. Through the development of ten years, the yield cannot break through 200 tons at the end of the 20 th century, the main market of the product is also the pharmaceutical industry, the product is mainly used for various amino acid infusion, the amino acid infusion is commonly used for the body recovery before and after the operation, the adjuvant therapy of malnutrition, chronic consumptive diseases and the like, and the product is a variety with large clinical dosage. In recent years, domestic manufacturers mostly supply domestic requirements for threonine, and with the wide application of threonine in the international market, some domestic famous feed enterprises begin to apply threonine in feeds in large quantities so as to improve the quality of the feeds and further meet the requirements of the feed industry. The production mainly adopts a fermentation method, and mycoprotein and mother liquor generated in the production process are difficult to treat and have high cost, and byproducts with high cost performance are difficult to obtain.
Threonine is mainly produced by a fermentation method, and a large amount of threonine waste liquid is produced in the production process, contains pigments, sugar, inorganic salts, threonine, protein and other organic substances, and has the characteristics of high COD and high NH 3-N. The threonine fermentation waste refers to waste generated in the process of preparing threonine by microbial fermentation, and comprises mycoprotein and waste mother liquor. In the traditional production process, the threonine fermentation by-product mycoprotein is directly sold as cheap mycoprotein after flocculation precipitation and plate-frame filtration, and the added value of the product is low. The project carries out targeted enzymolysis on threonine mycoprotein, and hydrolysate contains a large amount of amino acids, abundant soluble proteins, polypeptides and the like so as to be used for replacing yeast powder as a nutrient substance to provide a nitrogen source for microorganisms.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides a comprehensive treatment process of threonine fermentation waste, which comprehensively and comprehensively treats the fermentation waste, prepares a microbial additive and can be applied to agricultural production increase and biocontrol.
The invention is realized by the following technical scheme.
The comprehensive treatment process of threonine fermentation waste comprises the following steps:
step 1) hydrolyzing mycoprotein; step 2), treating fermentation wastewater; step 3) preparing biocontrol bacterium liquid; and 4) preparing a fertilizer additive.
Further, the step 1) of hydrolyzing the mycoprotein comprises the following steps:
adding aqueous solution of calcium salt, manganese salt and zinc salt into mycoprotein to prepare thallus cell suspension with the concentration of 50g/L, homogenizing the thallus cell suspension by using a high-pressure homogenizer, stopping homogenizing, adjusting the temperature to 45 ℃, hydrolyzing by using neutral protease for 6 hours, then heating to 60 ℃, and preserving heat for 2 hours to obtain chelating solution.
Further, the step 2) for treating the fermentation wastewater comprises the following steps: adding sorghum straws into a pulverizer for pulverizing, sieving with a 50-mesh sieve to obtain sorghum straw powder, sequentially adding the sorghum straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12h, then placing in a granulator for granulation, placing particles in a tubular furnace, carbonizing in a nitrogen atmosphere, controlling the temperature at 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized particles.
Further, the step 3) of preparing the biocontrol bacterium liquid comprises the following steps:
culturing Streptomyces viridis and Bacillus licheniformis to density of 1 × 1010And mixing the bacterial liquid according to the volume ratio of 1:1 to obtain the biocontrol bacterial liquid.
Further, the step 4) of preparing the fertilizer additive comprises the following steps:
and uniformly stirring the biocontrol bacteria liquid, the chelating liquid and the carbonized particles, and drying at low temperature until the water content is 10-15% to obtain the fertilizer additive.
Preferably, the concentrations of the calcium salt, the manganese salt and the zinc salt are all 0.05 mol/L.
Preferably, the calcium salt is calcium nitrate, the manganese salt is manganese sulfate, and the zinc salt is zinc sulfate.
Preferably, the parameters of the homogenization treatment are: homogenizing at 25 deg.C under 90Mpa for 3 times.
Preferably, the fermentation wastewater: sorghum straw powder: bentonite = 1L: 300 g: 200 g.
Preferably, the ratio of the biocontrol bacteria liquid to the chelating liquid to the carbonized particles is 1L to 2 kg.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the present invention will be described more clearly and completely below with reference to specific embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
The comprehensive treatment process of threonine fermentation waste comprises the following steps:
the mode of producing threonine fermentation waste (including fermentation wastewater and mycoprotein) includes, for example: separating the mycoprotein in the threonine fermentation liquor by using a high-speed disc separator, recovering the mycoprotein, and collecting the supernatant; extracting threonine from the supernatant through membrane filtration, concentration and isoelectric precipitation to generate wastewater, namely fermentation wastewater;
adding water solution of calcium salt, manganese salt and zinc salt into thallus protein to prepare thallus cell suspension with concentration of 50g/L, and homogenizing the thallus cell suspension by using a high-pressure homogenizer at a homogenizing pressure of 90MPa and a temperature of 25 ℃ for 3 times; stopping homogenizing, adjusting the temperature to 45 ℃, hydrolyzing by using neutral protease, wherein the addition amount is 2000u enzyme activity/L, the enzymolysis time is 6h, then heating to 60 ℃, and preserving heat for 2h to obtain chelating solution;
the concentrations of the calcium salt, the manganese salt and the zinc salt are all 0.05 mol/L;
calcium salt is calcium nitrate; manganese sulfate is selected as manganese salt; zinc salt is zinc sulfate;
adding sorghum straws into a pulverizer for pulverizing, sieving with a 50-mesh sieve to obtain sorghum straw powder, and then sequentially adding the sorghum straw powder and bentonite into fermentation wastewater, wherein the addition amount is fermentation wastewater: sorghum straw powder: bentonite = 1L: 300 g: 200g, uniformly mixing, standing for 12h, then placing in a granulator for granulation (the particle size is 1 mm), placing the granules in a tube furnace, carbonizing in a nitrogen atmosphere at 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized granules;
culturing Streptomyces viridis ATCC29814 and Bacillus licheniformis ATCC25972 to density of 1 × 1010Mixing the bacteria liquid according to the volume ratio of 1:1 to obtain biocontrol bacteria liquid;
uniformly stirring the biocontrol bacteria solution, the chelating solution and the carbonized particles, and drying at low temperature (15-25 ℃) until the water content is 10-15% to prepare a fertilizer additive; the ratio of the biocontrol bacteria liquid to the chelating liquid to the carbonized particles is 1L to 2 kg.
Example 2
The microorganism used for threonine fermentation is escherichia coli engineering bacteria.
Determination of total nitrogen: measuring total nitrogen by adopting a Kjeldahl method, wherein the total nitrogen is counted as N1;
determination of amino acid nitrogen: the amino acid nitrogen is measured by formaldehyde titration and is counted as N2.
Degree of hydrolysis: (N2/N1). times.100%.
The effect of high pressure homogenization on the degree of hydrolysis was examined. See table 1 specifically:
TABLE 1
| Number of times of high-pressure homogenization | Cell disruption rate% | Degree of hydrolysis% |
| 1 | 78.1 | 3.4 |
| 2 | 87.9 | 4.9 |
| 3 | 95.6 | 6.1 |
| 4 | 96.1 | 6.2 |
Increase high pressure homogenate number of times, cell breakage rate and degree of hydrolysis all improve to some extent, and the effect of 4 times homogenate only slightly promotes than 3 times, considers the damage of homogenate to equipment, and it is comparatively suitable to select 3 times homogenate.
Example 3
And (3) testing the performance of the amino acid metal chelating solution:
chelation rate (%) = (W1-W0) × 100/Wl;
w1: the total metal ion content;
w0: content of free metal ions.
Grouping:
experimental groups: example 1;
control group 1: the procedure of example 1 was otherwise the same;
control group 2: after the enzymolysis is finished, the same amount of metal ions are added, and the rest is the same as the example 1;
the specific results are shown in Table 2.
TABLE 2
| Index (I) | Chelate ratio (%) | Amino acid content g/L |
| The invention | 90.4 | 3.19 |
| Control group 1 | 72.9 | 2.03 |
| Control group 2 | 85.6 | 2.83 |
As shown in table 2 above, the homogenization treatment process is simple and convenient, the time is short, and more than 90% of cell walls of the somatic cells can be destroyed, which is beneficial to subsequent enzymolysis; according to the invention, calcium salt, manganese salt and zinc salt are added into the mycoprotein solution, so that osmotic pressure can be improved to promote thallus cracking, enzyme activity can be improved, the mycoprotein solution can be used as metal ions for amino acid chelation to prepare amino acid chelates, enzymolysis and chelation are carried out simultaneously, and cost and operation flow are saved.
Example 4
Influence of the carbonized particles on the adsorption amount of the chelate and the thallus.
The comparison was performed using a variety of common adsorbents.
Control group 1: bentonite;
control group 2: sorghum straw powder;
control group 3: diatomaceous earth.
The specific adsorption amounts of the carbonized particles to the chelate and the bacteria are shown in Table 3.
TABLE 3
| Group of | Chelate adsorption capacity (mg/g adsorbent) | Adsorption amount of bacteria (mg/g adsorbent) |
| Control group 1 | 0.27 | 10.4 |
| Control group 2 | 0.35 | 8.7 |
| Control group 3 | 0.59 | 14.9 |
| The invention | 1.12 | 21.8 |
As shown in the above table 3, the carbonized particles prepared by the present invention utilize fermentation wastewater and sorghum straws, and through the addition of bentonite, the adsorption effect is enhanced, amino acid chelates and thalli can be adsorbed by coordination, hydrogen bonds, coulomb force and other manners, so that the defects of amino acid loss and too fast release along with water are avoided, and the carbonized particles can be finally fully utilized by plants to provide nutrients for the plants; the biological control bacteria has larger carrying capacity and is beneficial to preventing and controlling crop diseases and insect pests.
Although the present invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the present invention. Accordingly, it is intended that all such modifications and variations as fall within the true spirit of this invention be included within the scope thereof.
Claims (10)
1. The comprehensive treatment process of threonine fermentation waste comprises the following steps:
step 1) hydrolyzing mycoprotein; step 2), treating fermentation wastewater; step 3) preparing biocontrol bacterium liquid; and 4) preparing a fertilizer additive.
2. The process of claim 1, wherein the step 1) of hydrolyzing mycoprotein comprises the steps of:
adding aqueous solution of calcium salt, manganese salt and zinc salt into mycoprotein to prepare thallus cell suspension with the concentration of 50g/L, homogenizing the thallus cell suspension by using a high-pressure homogenizer, stopping homogenizing, adjusting the temperature to 45 ℃, hydrolyzing by using neutral protease for 6 hours, then heating to 60 ℃, and preserving heat for 2 hours to obtain chelating solution.
3. The process as claimed in claim 2, wherein the step 2) of treating the fermentation wastewater comprises the steps of: adding sorghum straws into a pulverizer for pulverizing, sieving with a 50-mesh sieve to obtain sorghum straw powder, sequentially adding the sorghum straw powder and bentonite into fermentation wastewater, uniformly mixing, standing for 12h, then placing in a granulator for granulation, placing particles in a tubular furnace, carbonizing in a nitrogen atmosphere, controlling the temperature at 500 ℃ for 30min, taking out, and naturally cooling to obtain carbonized particles.
4. The process as claimed in claim 3, wherein the step 3) of preparing the biocontrol bacteria liquid comprises the following steps:
culturing Streptomyces viridis and Bacillus licheniformis to density of 1 × 1010And mixing the bacterial liquid according to the volume ratio of 1:1 to obtain the biocontrol bacterial liquid.
5. The process of claim 4, wherein step 4) of preparing a fertilizer additive comprises the steps of:
and uniformly stirring the biocontrol bacteria liquid, the chelating liquid and the carbonized particles, and drying at low temperature until the water content is 10-15% to obtain the fertilizer additive.
6. The process according to claim 2, wherein the concentrations of the calcium salt, manganese salt and zinc salt are all 0.05 mol/L.
7. The process as claimed in claim 2, wherein the calcium salt is calcium nitrate, the manganese salt is manganese sulfate, and the zinc salt is zinc sulfate.
8. The process according to claim 2, characterized in that the parameters of the homogenization treatment are: homogenizing at 25 deg.C under 90Mpa for 3 times.
9. The process of claim 3, wherein the fermentation wastewater: sorghum straw powder: bentonite = 1L: 300 g: 200 g.
10. The process of claim 5, wherein the ratio of the biocontrol bacteria liquid to the chelating liquid to the carbonized particles is 1L to 2 kg.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112409057A (en) * | 2020-12-30 | 2021-02-26 | 赵兰坤 | Method for preparing organic fertilizer by using amino acid fermentation waste |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112409057A (en) * | 2020-12-30 | 2021-02-26 | 赵兰坤 | Method for preparing organic fertilizer by using amino acid fermentation waste |
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