CN103966298A - Recycling method of tetracycline fungi residue - Google Patents
Recycling method of tetracycline fungi residue Download PDFInfo
- Publication number
- CN103966298A CN103966298A CN201410221646.2A CN201410221646A CN103966298A CN 103966298 A CN103966298 A CN 103966298A CN 201410221646 A CN201410221646 A CN 201410221646A CN 103966298 A CN103966298 A CN 103966298A
- Authority
- CN
- China
- Prior art keywords
- tsiklomitsin
- bacterium slag
- starch
- secondary fermentation
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention relates to a recycling method of tetracycline fungi residue. The method applies tetracycline fungi residue secondary fermentation liquor or enzymatic hydrolysate thereof or extract prepared by separating hydrolysate as a substitute of organic nitrogen sources such as yeast powder or corn pulp, and the like, in a tetracycline fermentation system, so that tetracycline fungi residue emission load is reduced, and therefore, a purpose of recycling the fungi residue is achieved. The method disclosed by the invention can lower fungi residue treatment cost, can lower material cost of the tetracycline fermentation, and improves fermentation level at the same time. Compared with the existing tetracycline fermentation process, on the condition that a fermentation process control process is not changed, the recycling method disclosed by the invention grows 160-180hr placing tanks, has titer of 31000-32500 mu/ml, improves fermentation titer by over 8%, lowers cost by over 30%, applies a great deal of tetracycline fungi residue and reduces emission load.
Description
Technical field
The present invention relates to biological and medical technical field, particularly relate to a kind of circulation utilization method of tsiklomitsin bacterium slag.
Background technology
Bacterium slag is containing of producing in production of antibiotics process to remain substratum and broken mycelial refuse, in tsiklomitsin fermentation production process, produce a large amount of bacterium slags, it is with starch, soybean cake powder, the waste residue that the organic carbon nitrogenous sources such as yeast powder produce after fermenting process, except containing a large amount of moisture, in tsiklomitsin bacterium slag, contain abundant carbon source, nitrogenous source, VITAMIN, the nutritive substances such as trace element, dry-matter accounts for more than 30%, total reducing sugar accounts for more than 15%, nitrogen class material accounts for more than 50% (butt meter), but due to the impact of its residual microbiotic and metabolic intermediate etc., it is again a kind of special danger wastes that tsiklomitsin bacterium slag is not only a kind of nutritive substance simultaneously, if can not get rational disposal, can bring harm to human health and ecological environment security, its harm has the features such as disguised and hysteresis quality.
Chinese patent " a kind for the treatment of process of tsiklomitsin bacterium slag " (application number is 201310527166.4) provides a kind of second fermentation process of tsiklomitsin bacterium slag, can obtain nutritious organic nitrogen source by this harmless treatment mode, in this patent, pointed out can using the surrogate of this organic nitrogen source yeast powder or corn steep liquor in tsiklomitsin fermenting process or dry after as feed or Fertilizer application, reach the recycle of bacterium slag, reduce the discharge of bacterium slag, turn waste into wealth, cost-saving effect.But do not set forth with regard to the concrete application mode of this organic nitrogen source material in this patent, if be especially back to use in tsiklomitsin fermentation system, how is feasibility? fermentation titer is there to impact? can which type of condition reach the requirement that improves fermentation titer? at present, the research of the recycle to tsiklomitsin bacterium slag is substantially without referential mature technology.
Summary of the invention
The object of the present invention is to provide one to make full use of tsiklomitsin waste residue, reduce tsiklomitsin fermentation residue quantity discharged, reach the recycle object of bacterium slag, reduce bacterium slag processing cost, reduce the material cost of tsiklomitsin fermentation, improve the circulation utilization method of the tsiklomitsin bacterium slag of fermentation level.
For realizing the technical scheme that foregoing invention order takes be:
A circulation utilization method for tsiklomitsin bacterium slag, is characterized in that: using the raw material integral part of fermention medium and the supplemented medium in tsiklomitsin fermentation system of the tsiklomitsin bacterium slag Secondary Fermentation liquid after deactivation, wherein
Fermention medium consists of: starch 6～8%, soybean cake powder 1.5～2.5%, tsiklomitsin bacterium slag Secondary Fermentation liquid (giving money as a gift) after deactivation: 2.0～3.0%, corn steep liquor 1.0～1.5%, potassium primary phosphate 0.02～0.04%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15～0.25%;
Supplemented medium consists of: starch 35～50%, and the Secondary Fermentation liquid (giving money as a gift) of the tsiklomitsin bacterium slag after deactivation: 1.0～2.0%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%;
Aforementioned proportion is in mass volume ratio.
A circulation utilization method for tsiklomitsin bacterium slag, is characterized in that: using after tsiklomitsin bacterium slag Secondary Fermentation liquid enzymolysis processing in tsiklomitsin fermentation system the raw material integral part of fermention medium and supplemented medium, wherein
Fermention medium consists of: starch 6～9%, soybean cake powder 2～2.5%, the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 2.0～3.0%, potassium primary phosphate 0.03～0.06%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15-0.25%;
Supplemented medium consists of: starch 35～50%, and the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 1.5～2.0%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%;
Aforementioned proportion is in mass volume ratio.
A kind of circulation utilization method of tsiklomitsin bacterium slag, it is characterized in that: the raw material integral part of yeast extract seed culture medium, fermention medium and the supplemented medium in tsiklomitsin fermentation system of making after the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid separates, wherein
Seed culture medium consists of: starch 3～5%, soybean cake powder 1.5～3%, the yeast extract (giving money as a gift) 0.8～1.2% that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating, potassium primary phosphate 0.01～0.025%, calcium carbonate 0.2～0.4%, ammonium sulfate 0.4～0.6%;
Fermention medium consists of: starch 6～9%, soybean cake powder 1.5～2.0%, the yeast extract (giving money as a gift) that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating: 1.5～3.0%, potassium primary phosphate 0.03～0.06%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15～0.25%;
The composition of supplemented medium: starch 35～50%, the yeast extract (giving money as a gift) that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating: 1.0～1.5%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%.
Existing tsiklomitsin fermentation adopts second order fermentation, in fermenting process, fills into carbon nitrogen mixture according to fermented liquid residual sugar level, fills into ammoniacal liquor according to PH level.Wherein
Seed culture based formulas (mass volume ratio): starch 3～5%, soybean cake powder 1.5～3%, yeast powder 0.5～1.5%, corn steep liquor 0.5～1.0%, potassium primary phosphate 0.01～0.025%, calcium carbonate 0.2～0.4%, ammonium sulfate 0.4～0.6%.
Seed culture condition: 30～33 DEG C of temperature, air flow 1:1.2～1.5V/V/min, stirs, and the approximately 26～30hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 7～10%, soybean cake powder 2～3.5%, yeast powder 0.5～2.0%, corn steep liquor 1.5～3.0%, potassium primary phosphate 0.01～0.02%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15～0.25%.
Fermentation culture conditions: 30～33 DEG C of temperature, air flow 1:1.2～1.5V/V/min, stirs, control dissolved oxygen 20～50%, fermenting process fills into carbon nitrogen mixture according to remaining sugar concentration, and controlled fermentation liquid residual sugar is in 4.0～2.5% left and right, fill into ammoniacal liquor according to PH, control PH 5.6～6.0.
Carbon nitrogen mixture proportioning (mass volume ratio): starch 35～50%, yeast powder 0.2～0.5%, corn steep liquor 1.0～2.5%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%.
By above-mentioned technique, final fermentation culture 160～180hr is put tank, and 27000～29000u/ml tires.
The present invention is by carrying out Secondary Fermentation by tsiklomitsin bacterium slag, albumen and total glucides material in bacterium slag are degraded, and cultivate a large amount of yeast, obtain after treatment the abundanter organic nitrogen source of a kind of aminoacids content, be back in tsiklomitsin fermentation system, as the surrogate of the organic nitrogen sources such as yeast powder or corn steep liquor in tsiklomitsin fermenting process, thereby reduce tsiklomitsin fermentation residue quantity discharged, reached the recycle object of bacterium slag.Can reduce bacterium slag processing cost by method of the present invention, reduce the material cost of tsiklomitsin fermentation, improve fermentation level simultaneously.Compared with existing tetracycline fermentation process, in the situation that zymotechnique control process is constant, the present invention 160～180hr that grows is put tank, 31000～the 32500u/ml that tires, fermentation titer improves more than 8%, reduces costs more than 30%, use a large amount of tsiklomitsin bacterium slags, reduce quantity discharged.
Embodiment
Comparative example 1:
Seed culture based formulas (mass volume ratio): starch 4.0%, soybean cake powder 2.8%, yeast powder 1.3%, corn steep liquor 0.7%, potassium primary phosphate 0.01%, calcium carbonate 0.38%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
33 DEG C of temperature, air flow 1:1.4V/V/min, stirs, and the about 29hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 8.5%, soybean cake powder 3.0%, yeast powder 1.8%, corn steep liquor 2.5%, potassium primary phosphate 0.01%, calcium carbonate 0.6%, ammonium sulfate 0.7%, Sodium Bromide 0.2%.
Fermentation culture conditions: 33 DEG C of temperature, 60hr is cooled to 32 DEG C, air flow 1:1.3V/V/min, stir, control dissolved oxygen 20-50%, fermenting process fills into carbon nitrogen mixture according to remaining sugar concentration, controlled fermentation liquid residual sugar, at 3.5-2.5%, fills into ammoniacal liquor according to PH, controls PH at 5.7-5.9.
Carbon nitrogen mixture proportioning (mass volume ratio): starch 40%, yeast powder 0.5%, corn steep liquor 2.0%, calcium carbonate 0.6%, Sodium Bromide 0.15%.
Fermentation culture 170hr is put tank, and 28930u/ml tires.
Comparative example 2:
Seed culture based formulas (mass volume ratio): starch 3.5%, soybean cake powder 2.5%, yeast powder 1.2%, corn steep liquor 0.8%, potassium primary phosphate 0.015%, calcium carbonate 0.4%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
32 DEG C of temperature, air flow 1:1.4V/V/min, stirs, and the about 30hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 8.0%, soybean cake powder 2.5%, yeast powder 1.5%, corn steep liquor 2.8%, potassium primary phosphate 0.015%, calcium carbonate 0.65%, ammonium sulfate 0.6%, Sodium Bromide 0.25%.
Fermentation culture conditions: 33 DEG C of temperature, 70hr is cooled to 32 DEG C, air flow 1:1.4V/V/min, stir, control dissolved oxygen 30-50%, fermenting process fills into carbon nitrogen mixture according to remaining sugar concentration, controlled fermentation liquid residual sugar, at 4.0-3.0%, fills into ammoniacal liquor according to PH, controls PH at 5.7-5.9.
Carbon nitrogen mixture proportioning: starch 38%, yeast powder 0.8%, corn steep liquor 1.8%, calcium carbonate 0.6%, Sodium Bromide 0.15%
Fermentation culture 170hr is put tank, and 29080u/ml tires.
Embodiment 1:
The enzymolysis solution Free Amino Acids total amount 4280mg/100ml of tsiklomitsin bacterium slag tsiklomitsin bacterium slag Secondary Fermentation of gained after Secondary Fermentation, enzymolysis.
Seed culture medium proportioning (mass volume ratio): starch 3.5%, soybean cake powder 2.5%, yeast powder 1.0%, corn steep liquor 0.75%, potassium primary phosphate 0.015%, calcium carbonate 0.3%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
33 DEG C of temperature, air flow 1:1.3V/V/min, stirs, and the about 28hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 7%, soybean cake powder 2%, the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation: 2.8%, potassium primary phosphate 0.03%, calcium carbonate 0.5%, ammonium sulfate 0.6%, Sodium Bromide 0.15%.
Supplemented medium (mass volume ratio): starch 40%, the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation: 1.5%, calcium carbonate 0.5%, Sodium Bromide 0.15%.
Other conditions are with existing tetracycline fermentation process, and in fermenting process, according to fermented liquid residual sugar control carbon nitrogen source mixture, growth 168hr is put tank, the 30680u/ml that tires, fermentation titer improves more than 6%, reduces costs more than 22%, use a large amount of tsiklomitsin bacterium slags, reduce bacterium slag quantity discharged.
Embodiment 2:
Tsiklomitsin bacterium slag concentrated medicinal extract total amino acid content 7650mg/100ml after Secondary Fermentation enzymolysis.
Seed culture based formulas (mass volume ratio): starch 3.5%, soybean cake powder 2.0%, the yeast extract (giving money as a gift) 1.0% that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating, potassium primary phosphate 0.015%, calcium carbonate 0.35%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
32 DEG C of temperature, air flow 1:1.2V/V/min, stirs, and the about 7hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 8.0%, soybean cake powder 1.7%, bacterium slag Secondary Fermentation separates concentrated extract (giving money as a gift): 2.8%, potassium primary phosphate 0.04%, calcium carbonate 0.65%, ammonium sulfate 0.6%, Sodium Bromide 0.2%.
Supplemented medium (mass volume ratio): starch 45%, the yeast extract (giving money as a gift) that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating: 1.25%, calcium carbonate 0.55%, Sodium Bromide 0.15%.
Other conditions are with existing tetracycline fermentation process, and in fermenting process, according to fermented liquid residual sugar control carbon nitrogen source mixture, growth 166hr is put tank, the 32240u/ml that tires, fermentation titer improves more than 10%, reduces costs more than 30%, use a large amount of tsiklomitsin bacterium slags, reduce bacterium slag quantity discharged.
Embodiment 3
Tsiklomitsin bacterium slag is gained tsiklomitsin bacterium slag Secondary Fermentation liquid Free Amino Acids total amount 1568mg/100ml after Secondary Fermentation deactivation.
Seed culture medium proportioning (mass volume ratio): starch 3.5%, soybean cake powder 2.5%, yeast powder 1.0%, corn steep liquor 0.75%, potassium primary phosphate 0.015%, calcium carbonate 0.3%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
33 DEG C of temperature, air flow 1:1.3V/V/min, stirs, and the about 28hr that grows moves into fermention medium.
Fermentative medium formula (mass volume ratio): starch 6%, soybean cake powder 1.5%, tsiklomitsin bacterium slag Secondary Fermentation liquid (giving money as a gift): 3.0%, corn steep liquor 1.0%, potassium primary phosphate 0.03%, calcium carbonate 0.5%, ammonium sulfate 0.6%, Sodium Bromide 0.15%
Supplemented medium (mass volume ratio): starch 35%, tsiklomitsin bacterium slag Secondary Fermentation liquid (giving money as a gift): 1.0%, calcium carbonate 0.5%, Sodium Bromide 0.15%.
Fermentation culture conditions: 33 DEG C of temperature, 70hr is cooled to 32 DEG C, and air flow 1:1.4V/V/min stirs, control dissolved oxygen 30-50%, according to fermented liquid residual sugar control carbon nitrogen source mixture, growth 168hr is put tank, and 31420u/ml tires, fermentation titer improves more than 8%, reduce costs more than 25%, use a large amount of tsiklomitsin bacterium slags, reduce bacterial classification quantity discharged.
Embodiment 4
The tsiklomitsin bacterium slag Secondary Fermentation liquid Free Amino Acids total amount 1760mg/100ml of tsiklomitsin bacterium slag gained after Secondary Fermentation deactivation.
Seed culture medium: starch 3.0%, soybean cake powder 2.2%, yeast powder 1.2%, corn steep liquor 0.6%, potassium primary phosphate 0.01%, calcium carbonate 0.3%, ammonium sulfate 0.5%.
To in seed culture medium good the slant pore access sterilizing of tsiklomitsin bacterial classification, cultivate by following condition:
33 DEG C of temperature, air flow 1:1.3V/V/min, stirs, and the about 27hr that grows moves into fermention medium.
Fermentative medium formula: starch 8%, soybean cake powder 2.5%, the deactivation liquid (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 2.0%, corn steep liquor 1.5%, potassium primary phosphate 0.02%, calcium carbonate 0.6%, ammonium sulfate 0.7%, Sodium Bromide 0.2%.
Supplemented medium: starch 45%, the deactivation liquid (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 2.0%, calcium carbonate 0.7%, Sodium Bromide 0.2%.
Fermentation culture conditions: 33 DEG C of temperature, 60hr is cooled to 32 DEG C, and air flow 1:1.4V/V/min stirs, control dissolved oxygen 30-50%, according to fermented liquid residual sugar control carbon nitrogen source mixture, growth 168hr is put tank, and 30850u/ml tires, fermentation titer improves more than 6%, reduce costs more than 20%, use a large amount of tsiklomitsin bacterium slags, reduce bacterial classification quantity discharged.
In above-described embodiment, the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid, tsiklomitsin bacterium slag Secondary Fermentation liquid all adopts the method for Chinese patent " a kind for the treatment of process of tsiklomitsin bacterium slag " (application number is 201310527166.4) to obtain.The enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is carried out after solid-liquid separation, and gained liquid is by the concentrated medicinal extract that obtains.
Claims (3)
1. a circulation utilization method for tsiklomitsin bacterium slag, is characterized in that: using the raw material integral part of fermention medium and the supplemented medium in tsiklomitsin fermentation system of the tsiklomitsin bacterium slag Secondary Fermentation liquid after deactivation, wherein
Fermention medium consists of: starch 6～8%, soybean cake powder 1.5～2.5%, tsiklomitsin bacterium slag Secondary Fermentation liquid (giving money as a gift) after deactivation: 2.0～3.0%, corn steep liquor 1.0～1.5%, potassium primary phosphate 0.02～0.04%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15～0.25%;
Supplemented medium consists of: starch 35～50%, and the Secondary Fermentation liquid (giving money as a gift) of the tsiklomitsin bacterium slag after deactivation: 1.0～2.0%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%;
Aforementioned proportion is in mass volume ratio.
2. a circulation utilization method for tsiklomitsin bacterium slag, is characterized in that: using after tsiklomitsin bacterium slag Secondary Fermentation liquid enzymolysis processing in tsiklomitsin fermentation system the raw material integral part of fermention medium and supplemented medium, wherein
Fermention medium consists of: starch 6～9%, soybean cake powder 2～2.5%, the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 2.0～3.0%, potassium primary phosphate 0.03～0.06%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15-0.25%;
Supplemented medium consists of: starch 35～50%, and the enzymolysis solution (giving money as a gift) of tsiklomitsin bacterium slag Secondary Fermentation liquid: 1.5～2.0%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%;
Aforementioned proportion is in mass volume ratio.
3. the circulation utilization method of a tsiklomitsin bacterium slag, it is characterized in that: the raw material integral part of yeast extract seed culture medium, fermention medium and the supplemented medium in tsiklomitsin fermentation system of making after the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid separates, wherein
Seed culture medium consists of: starch 3～5%, soybean cake powder 1.5～3%, the yeast extract (giving money as a gift) 0.8～1.2% that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating, potassium primary phosphate 0.01～0.025%, calcium carbonate 0.2～0.4%, ammonium sulfate 0.4～0.6%;
Fermention medium consists of: starch 6～9%, soybean cake powder 1.5～2.0%, the yeast extract (giving money as a gift) that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating: 1.5～3.0%, potassium primary phosphate 0.03～0.06%, calcium carbonate 0.5～0.7%, ammonium sulfate 0.6～0.8%, Sodium Bromide 0.15～0.25%;
The composition of supplemented medium: starch 35～50%, the yeast extract (giving money as a gift) that the enzymolysis solution of tsiklomitsin bacterium slag Secondary Fermentation liquid is made after separating: 1.0～1.5%, calcium carbonate 0.5～0.7%, Sodium Bromide 0.1～0.2%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410221646.2A CN103966298A (en) | 2014-05-23 | 2014-05-23 | Recycling method of tetracycline fungi residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410221646.2A CN103966298A (en) | 2014-05-23 | 2014-05-23 | Recycling method of tetracycline fungi residue |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103966298A true CN103966298A (en) | 2014-08-06 |
Family
ID=51236300
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410221646.2A Pending CN103966298A (en) | 2014-05-23 | 2014-05-23 | Recycling method of tetracycline fungi residue |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103966298A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151381A (en) * | 2021-04-26 | 2021-07-23 | 山东胜利生物工程有限公司 | Method for preparing nosiheptide fermentation liquor |
| CN114292755A (en) * | 2021-12-06 | 2022-04-08 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation of antibiotic fungi residue lysate |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463320A (en) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | Biological nitrogen, and preparation and use thereof |
| CN102586115A (en) * | 2012-03-28 | 2012-07-18 | 宁夏大学 | Method for aerobically producing yeast extract by using tetracycline fungi residues |
| CN102864202A (en) * | 2012-10-19 | 2013-01-09 | 河北圣雪大成制药有限责任公司 | Method for producing tetracycline through fermenting |
| CN103555780A (en) * | 2013-10-31 | 2014-02-05 | 宁夏启元药业有限公司 | Method for processing tetracycline bacterial dreg |
| CN103602714A (en) * | 2013-11-20 | 2014-02-26 | 宁夏启元药业有限公司 | Method for producing tetracycline by fermentation of streptomyces aureus |
-
2014
- 2014-05-23 CN CN201410221646.2A patent/CN103966298A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101463320A (en) * | 2007-12-17 | 2009-06-24 | 华北制药集团有限责任公司 | Biological nitrogen, and preparation and use thereof |
| CN102586115A (en) * | 2012-03-28 | 2012-07-18 | 宁夏大学 | Method for aerobically producing yeast extract by using tetracycline fungi residues |
| CN102864202A (en) * | 2012-10-19 | 2013-01-09 | 河北圣雪大成制药有限责任公司 | Method for producing tetracycline through fermenting |
| CN103555780A (en) * | 2013-10-31 | 2014-02-05 | 宁夏启元药业有限公司 | Method for processing tetracycline bacterial dreg |
| CN103602714A (en) * | 2013-11-20 | 2014-02-26 | 宁夏启元药业有限公司 | Method for producing tetracycline by fermentation of streptomyces aureus |
Non-Patent Citations (3)
| Title |
|---|
| 惠兴育: "利用四环素菌渣好氧生产酵母浸膏的应用研究", 《宁夏大学论文集》 * |
| 李月海等: "抗生物菌渣的综合利用", 《山东畜牧兽医》 * |
| 蔡翔: "可利霉素菌渣作为氮源的再利用研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113151381A (en) * | 2021-04-26 | 2021-07-23 | 山东胜利生物工程有限公司 | Method for preparing nosiheptide fermentation liquor |
| CN114292755A (en) * | 2021-12-06 | 2022-04-08 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation of antibiotic fungi residue lysate |
| CN114292755B (en) * | 2021-12-06 | 2024-01-26 | 内蒙古常盛制药有限公司 | Method for recycling penicillin fermentation from antibiotic fungus dreg lysate |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103602714B (en) | A kind of method of streptomyces aureus fermenting and producing tetracycline | |
| CN101709272B (en) | Method for preparing feed yeast | |
| CN101748161A (en) | Succinic acid purification process through anaerobic fermentation | |
| CN104355759A (en) | Garden flower/tree matrix fertilizer produced from antibiotic bacterium slag and preparation method thereof | |
| CN103848699B (en) | A kind of cultivation culture material of the Ji mushroom containing oil-tea camellia husks and making method | |
| CN102951966A (en) | Production method for compound microbial manure | |
| CN101747093A (en) | Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C | |
| CN103695519A (en) | Nitrogen source, culture medium and culture method for producing riboflavin | |
| CN104876761A (en) | Organic biological fertilizer special for codonopsis pilosula | |
| CN109429911A (en) | A kind of fermentation cordyceps sinensis culture solution and preparation method thereof prepared with jerusalem artichoke | |
| CN106591375A (en) | Production technology for microbial activated liquid | |
| CN105085023A (en) | Foliar fertilizer prepared through Chinese medicine residue fermentation and preparation method thereof | |
| CN103204713A (en) | Method for promoting high efficiency decomposition of enteromorpha powder by using enzymatically hydrolyzed sea-tangle residues and application of product | |
| CN106348817A (en) | Process for preparing liquid bio-fertilizer by using corn starch sugar wastes | |
| CN103642885A (en) | Method for producing wheat biological active peptide by fermentation | |
| CN113817635A (en) | Method for culturing bacillus by using soybean whey wastewater | |
| CN101390567A (en) | Production method of DDS biology protein feedstuff | |
| CN103304308A (en) | Lysine biological salt decomposition bacteria mixture, organic fertilizer thereof and preparation methods of lysine biological salt decomposition bacteria mixture and organic fertilizer | |
| CN103966298A (en) | Recycling method of tetracycline fungi residue | |
| CN109234334A (en) | A kind of method of fermenting and producing tremella polysaccharides and fermentation medium used | |
| CN101302476A (en) | Method for preparing microorganism fermentation medium by means of biogas fluid | |
| CN105152805A (en) | Mineral foliar fertilizer suitable for fruit trees and preparation method of fertilizer | |
| CN102787153B (en) | Method for producing enramycin by microbial fermentation supplement feed | |
| CN105110899A (en) | Humic acid foliar fertilizer and preparation method therefor | |
| CN102389024A (en) | Method for producing feeding peanut peptide by anaerobic fermentation of peanut meal |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140806 |
|
| RJ01 | Rejection of invention patent application after publication |