CN101747093A - Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C - Google Patents
Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C Download PDFInfo
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- CN101747093A CN101747093A CN200910248459A CN200910248459A CN101747093A CN 101747093 A CN101747093 A CN 101747093A CN 200910248459 A CN200910248459 A CN 200910248459A CN 200910248459 A CN200910248459 A CN 200910248459A CN 101747093 A CN101747093 A CN 101747093A
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- organic fertilizer
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- tropina
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
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Abstract
The invention relates to a method for producing mycoprotein and polypeptide organic fertilizer by waste fermented mash and gulonic acid mother solution formed in the vitamin C production technology, which belongs to the field of biotechnology and waste resource utilization technology, and comprises the following steps: adding 5 to 20 parts of gulonic acid mother solution in 100 parts of waste vitamin C fermented mash protein, adjusting pH value to 2.0 to 5.0, and hydrolyzing for 0.5 to 12 hours under the temperature of 40 to 70 DEG C. On the one hand part of protein in fermented mash protein is hydrolyzed to become polypeptide and amino acid to dissolve in water, through adjusting pH value and appropriate concentration, right amount of trace element is added, obtaining polypeptide organic fertilizer, on the other hand the rest protein in mash protein is precipitated, and the precipitated protein is made into mycoprotein through filter-pressing, drying and smashing. The invention recycles the vitamin C fermentation industry waste to produce mycoprotein and polypeptide organic fertilizer, thus not only solving the problem of industrial pollution, but also providing organic fertilizer and feed protein for agricultural production.
Description
Technical field
The present invention relates to a kind of method of producing tropina and polypeptide organic fertilizer, belong to biotechnology and utilization of waste as resource technical field with discarded fermentation liquid of vitamins C and gulonic acid mother solution.
Background technology
Tropina is microbial proteinous, single cell protein again.Press the raw materials for production difference, can be divided into petroprotein, methyl alcohol albumen, methane albumen etc.; By producing bacterial classification class difference, can be divided into bacterioprotein, mycoprotein etc. again.In the whole world single cell protein meeting first time, the microbial bacteria body protein was referred to as single cell protein in 1967.The contained nutritive substance of single cell protein is abundant, and wherein, protein content is up to 40wt%～80wt%, and is higher by 10%～20% than soybean, and is higher more than 20% than meat, fish, cheese; It is comparatively complete that amino acid is formed, and contains the less Methionin of content in 8 seed amino acids, the especially cereal of needed by human.Also contain multivitamin, carbohydrate, lipid, mineral substance in the single cell protein, and abundant enzyme and biologically active substance, as: coenzyme A, ubiquinone, gsh, ergosterol etc.
At present, the microbe species that can be used for the manufacture order cell protein is a lot, comprises bacterium, actinomycetes, yeast, mould and some protobiont.These microorganisms need possess following condition usually: 1) the nutrition content height of being produced such as protein, do not have pathogenic effects to human body, delicious and easy absorption; 2) culture condition and production process are required simply, growth and breeding is rapid etc., and single cell protein has been widely used in food-processing and the feed.
Polypeptide is had nutrition and is regulated the small-molecule substance of physiological function by what amino acid was formed, is the biologically active substance that a class relates to various cell functions in the organism, has huge using value.Therefore, now be widely applied to human lives's every field, as: bio-pharmaceuticals, protective foods, bio-feritlizer, animal-feed etc.
Vitamins C claims xitix again, and it is prevalent in the vegetable and fruit, must additionally obtain from food.Now, China mainly adopts the artificial synthesise vitamins C of biological fermentation process.But producing two kinds of wastes that quantity is bigger in the Vc biological fermentation process production technique, is respectively mash albumen and gulonic acid mother solution, and its main characteristic is described below:
The bio-fermentation process that discarded mash albumen: Vc production is adopted gives off a large amount of waste fermented mash albumen.This waste fermented mash is beige, slightly thick, COD value 7-10 ten thousand mg/L, be that Vc fermentation liquid process ultra-filtration membrane in the subsequent extracted step is held back the macromolecular substance more than remaining 100,000 dalton in back, main component is tropina and residue substratum etc., so claim mash albumen.Treatment process in the past that should discarded mash is difficult to practical application because of power consumption, poor efficiency or seriously polluted etc.Therefore, both waste resource, also polluted environment.
Also produce a large amount of discarded gulonic acid mother solutions during discarded gulonic acid mother solution: Vc produces, to be the Vc bio-fermented liquid concentrate and the residue raffinate after respectively going on foot operation such as ancient imperial acid crystal extraction through centrifugal, ultrafiltration, ion exchange column exchange, triple effect evaporation, secondary this gulonic acid mother solution.Mainly contain formic acid, acetate, oxalic acid, the acid of ancient dragon, sorbose, protein, nucleic acid etc. in the gulonic acid mother solution.This waste is brownish black, is thick that the COD value reaches 1 * 10
6Mg/L, the pH value is then less than 1.0.At present, this gulonic acid mother solution falls as waste discharge, serious environment pollution.
How these two kinds of wastes being carried out recycling then is a current technical barrier.
Summary of the invention
The object of the present invention is to provide a kind of method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C, two kinds of important wastes of vitamin c fermenting industry: mash albumen and gulonic acid mother solution give recycling, produce the tropina and the polypeptide organic fertilizer that can be used as commodity, thereby both solved the industrial pollution problem, provided organic fertilizer and feedstuff protein for agriculture production again.
The technical solution used in the present invention is:
A kind of method of producing tropina and polypeptide organic fertilizer with fermentation wastes, meter adds the discarded gulonic acid mother solution of 5-20 part in the discarded mash albumen of 100 parts of vitamins Cs by weight, reconciles the pH value to 2.0-5.0; Then, under 40-90 ℃ of condition, be incubated 0.5-12 hour, this process is that mild hydrolysis is held concurrently and to be precipitated with time variation under acidic conditions.On the one hand, the partial protein in the mash albumen is hydrolyzed, and becomes polypeptide and amino acid is soluble in water, adopting Wingdale, unslaked lime or slaked lime etc. to reconcile the pH value is 6-8, through adding an amount of trace element and crop material, drying or airing more promptly becomes polypeptide organic fertilizer.
The polypeptide organic fertilizer that the present invention is made, organic content are 40～60wt%, and wherein: polypeptide and aminoacids content are 8～15wt%; Total nutrient (full nitrogen+P
2O
5+ KCl) be 4～7wt%, micronutrient levels 2～10wt%, pH6-8, its technical performance index meet organic fertilizer national sector standard (NY525-2002).
Among the present invention, the full nitrogen in the total nutrient, P
2O
5With the weight ratio of KCl be 1: (0.1～0.5): (0.1～0.5).
Among the present invention, trace element adds as required, can be one or more of Fe, Zn, Mn, Mo, B, and by weight percentage, 2%≤Fe+Zn+Mn+Mo+B 10%.
And on the other hand, the opposing party's partial protein in the mash albumen is then precipitated to get off, and the protein of post precipitation promptly is made for tropina through press filtration, drying, pulverizing.
The tropina that the present invention is made, its protein content are 45～75wt%, and water content is 10～15wt%.
Waste in the discarded mash albumen of the present invention bio-fermentation process that production is adopted from Vc, this waste fermented mash is beige, slightly thick, COD value 7-10 ten thousand mg/L, be that Vc fermentation liquid process ultra-filtration membrane in the subsequent extracted step is held back the macromolecular substance more than remaining 100,000 dalton in back, dry matter content in the discarded mash albumen is at 8-20wt%, be mainly tropina and residue substratum etc., the tropina in the dry-matter accounts for 45～75wt%.
Discarded gulonic acid mother solution of the present invention is from the Vc production technique, and being the Vc bio-fermented liquid concentrates and the residue raffinate after respectively going on foot operation such as ancient imperial acid crystal extraction through centrifugal, ultrafiltration, ion exchange column exchange, triple effect evaporation, secondary.Essential substance is formic acid, acetate, oxalic acid, the acid of ancient dragon, sorbose, protein and nucleic acid in the gulonic acid mother solution, and the gross weight of the formic acid in the gulonic acid mother solution, acetate, oxalic acid, the acid of ancient dragon, sorbose, protein and nucleic acid accounts for 60-96wt%.In the main component of above-mentioned gulonic acid mother solution, formic acid accounts for 30～45wt%, and acetate accounts for 10～30wt%, oxalic acid accounts for 10～15wt%, and ancient imperial acid accounts for 15～25wt%, and sorbose accounts for 2～10wt%, protein accounts for 1～5wt%, and nucleic acid accounts for 0.2～2wt%.This waste is brownish black, is thick that the COD value reaches 1 * 10
6Mg/L, the pH value is 0.6～1.5.
The present invention has following advantage:
1. the present invention makes the proteic pH value of mash conditioning agent with gulonic acid mother solution, has both saved the chemical agent that is used to regulate the pH value originally, more organicly provides for follow-up polypeptide organic fertilizer production again.
The present invention simultaneously with mash albumen and two kinds of wastes of gulonic acid mother solution as raw material, can save the material cost in the production in a large number.
3. the present invention can produce two kinds of product-tropinas and polypeptide organic fertilizer simultaneously, makes starting material obtain greatly to utilize and fully transform.
4. the present invention utilizes factory's waste pollutant with the Organic farming development again and merges, and has realized doulbe-sides' victory.
Embodiment
Embodiment 1
Discarded mash protein 20 ton is pumped in the reactor, pump into 1 ton of discarded gulonic acid mother solution again, stirring back pH value is 5.0, and reactor is heated to 90 ℃, is incubated 1 hour, and keeps static.Supernatant liquor in the reactor is pumped (about 17 tons), through 40 kilograms of slaked lime Ca (OH)
2Transfer pH to 7.2, through evaporation concentration extremely: polypeptide and amino acid account for 9wt%, add an amount of trace element by the fertilizer standard again and crop material promptly becomes polypeptide organic fertilizer.In the present embodiment, trace element can be Fe, Zn, Mn, Mo and B, by weight percentage, and Fe 1.2%, Zn 0.8%, Mn 0.2%, Mo 0.1%, B 0.1%.Precipitation part in the reactor pumps into plate-and-frame filter press, after press filtration, Tumblies Dry, and promptly is made for tropina (about 1.5 tons) after the pulverizing.
By weight percentage, the organic content 45% in the polypeptide organic fertilizer of obtaining, wherein: polypeptide and aminoacids content 11%, total nutrient (full nitrogen+P
2O
5+ KCl) 5.6%, micronutrient levels 2.4%, pH6.9.By weight percentage, obtain protein content 65% in the tropina, water content 11%.
Wherein, full nitrogen, the P in the total nutrient
2O
5With the weight ratio of KCl be 1: 0.2: 0.1.
Embodiment 2
Discarded mash protein 20 ton is pumped in the reactor, pump into discarded 3 tons of the imperial acid mother liquors that account for again, stirring back pH value is 4.0, and reactor is heated to 50 ℃, is incubated 2 hours, and keeps static.Supernatant liquor in the reactor is pumped (about 18 tons), through 100 kilograms of niter tone pH to 7.2, through evaporation concentration extremely: polypeptide and amino acid account for 7.6wt%, add an amount of trace element by the fertilizer standard again and crop material promptly becomes polypeptide organic fertilizer.In the present embodiment, trace element can be Fe, Zn, Mn and B, by weight percentage, and Fe 1.0%, Zn 0.7%, Mn 0.1%, B 0.2%.Precipitation part in the reactor pumps into plate-and-frame filter press, after press filtration, Tumblies Dry, and promptly is made for tropina (about 1.0 tons) after the pulverizing.
By weight percentage, the organic content 47% in the polypeptide organic fertilizer of obtaining, wherein: polypeptide and aminoacids content 10%, total nutrient (full nitrogen+P
2O
5+ KCl) 6.8%, micronutrient levels 2.0%, pH7.2.By weight percentage, the tropina that obtains, its protein content 62%, water content 11%.
Wherein, full nitrogen, the P in the total nutrient
2O
5With the weight ratio of KCl be 1: 0.3: 0.15.
Embodiment 3
Discarded mash protein 20 ton is pumped in the reactor, pump into 2 tons of discarded gulonic acid mother solutions again, stirring back pH value is 3.0, and reactor is heated to 40 ℃, is incubated 12 hours, and keeps static.Supernatant liquor in the reactor is pumped (about 15 tons), through 65 kilograms of niter tone pH to 7.2, through evaporation concentration extremely: polypeptide and amino acid account for 10wt%, add an amount of trace element by the fertilizer standard again and crop material promptly becomes polypeptide organic fertilizer.In the present embodiment, trace element can be Fe, Mn and B, by weight percentage, and Fe 1.5%, Mn 0.7%, B 0.3%.Precipitation part in the reactor pumps into plate-and-frame filter press, after press filtration, Tumblies Dry, and promptly is made for tropina (about 1.7 tons) after the pulverizing.
By weight percentage, the organic content 44% in the polypeptide organic fertilizer of obtaining, wherein: polypeptide and aminoacids content 13%, total nutrient (full nitrogen+P
2O
5+ KCl) 6.0%, micronutrient levels 2.5%, pH6.7.By weight percentage, the tropina that obtains, its protein content 57%, water content 12%.
Wherein, full nitrogen, the P in the total nutrient
2O
5With the weight ratio of KCl be 1: 0.15: 0.2.
Claims (5)
1. a method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C is characterized in that, meter adds the discarded gulonic acid mother solution of 5-20 part in the discarded mash albumen of 100 parts of vitamins Cs by weight, reconciles the pH value to 2.0-5.0; Then, under 40-90 ℃ of condition, be incubated 0.5-12 hour; The gained supernatant liquor is pressed the standard of fertilizer and is reconciled the pH value to neutrality with after concentrating, and adds trace element again and makes polypeptide organic fertilizer; Gained precipitation part promptly is made for tropina through conventional press filtration, drying, pulverizing.
2. according to the described method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C of claim 1, it is characterized in that, discarded mash albumen is the waste in the vitamin C bio zymotechnique, and dry matter content is at 8-20wt%, and the tropina in the dry-matter accounts for 45～75wt%.
3. according to the described method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C of claim 1, it is characterized in that, discarded gulonic acid mother solution is the waste in the vitamin C bio zymotechnique, the pH value is 0.6～1.5, main component is formic acid, acetate, oxalic acid, the acid of ancient dragon, sorbose, protein, nucleic acid in the discarded gulonic acid mother solution, and its total amount accounts for 60～96wt%.
4. according to the described method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C of claim 1, it is characterized in that, organic content in the described polypeptide organic fertilizer is 40～60wt%, and wherein: polypeptide and aminoacids content are 8～15wt%, total nutrient: full nitrogen+P
2O
5+ KCl is 4～7wt%, micronutrient levels 2～10wt%, pH6-8.
5. according to the described method of producing tropina and polypeptide organic fertilizer with fermented waste of vitamin C of claim 1, it is characterized in that the protein content in the described tropina is 45～75wt%, water content is 10～15wt%.
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|---|---|---|---|
| CN2009102484592A CN101747093B (en) | 2009-12-16 | 2009-12-16 | Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C |
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|---|---|---|---|
| CN2009102484592A CN101747093B (en) | 2009-12-16 | 2009-12-16 | Method for producing mycoprotein and polypeptide organic fertilizer by fermented waste of vitamin C |
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| CN101747093A true CN101747093A (en) | 2010-06-23 |
| CN101747093B CN101747093B (en) | 2012-07-04 |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103506009A (en) * | 2013-10-09 | 2014-01-15 | 沈阳农业大学 | Deep dehydration technique of Vc two-step fermentation ultrafiltration waste slag liquid |
| CN104710232A (en) * | 2013-12-16 | 2015-06-17 | 中国科学院沈阳应用生态研究所 | Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof |
| CN105175188A (en) * | 2015-09-24 | 2015-12-23 | 辽宁普天科技有限公司 | Method for preparing fulvic acid calcium magnesium fertilizers |
| CN106010561A (en) * | 2016-06-08 | 2016-10-12 | 内蒙古阜丰生物科技有限公司 | Soil conditioning agent containing threonine fermentation waste |
| CN106242680A (en) * | 2016-08-02 | 2016-12-21 | 李红旗 | Aminoacid water soluble fertilizer prepared by a kind of mother solution utilizing fermentative Production riboflavin and preparation method thereof |
| CN109265215A (en) * | 2017-07-17 | 2019-01-25 | 何立肖 | The processing method of gulonic acid mother solution in a kind of VC production |
| CN109354521A (en) * | 2018-09-26 | 2019-02-19 | 广东省农业科学院农业资源与环境研究所 | A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof |
| CN110295206A (en) * | 2019-07-11 | 2019-10-01 | 沈阳中科微道生物科技有限公司 | A kind of method and its application using the fermented abandoned mash preparation association preparation of Vc |
| CN111000028A (en) * | 2019-12-30 | 2020-04-14 | 南通励成生物工程有限公司 | Animal feed and preparation method thereof |
| CN111995467A (en) * | 2020-09-08 | 2020-11-27 | 山东鲁维制药有限公司 | Method for producing mycoprotein and polypeptide organic fertilizer by using vitamin C fermentation waste |
| CN112142510A (en) * | 2019-06-28 | 2020-12-29 | 沈阳颐康环境生物科技开发有限公司 | Regulating solution for improving vitamin C content of crops and preparation method and application thereof |
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| CN1305811C (en) * | 2004-12-01 | 2007-03-21 | 中国科学院沈阳应用生态研究所 | Pentone liquid fertilizer produced with vitamin c fermenting and super filtering waste liquid and its preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103506009B (en) * | 2013-10-09 | 2016-09-07 | 沈阳农业大学 | The Deep dehydration technique of Vc two-step fermentation ultrafiltration waste liquid |
| CN103506009A (en) * | 2013-10-09 | 2014-01-15 | 沈阳农业大学 | Deep dehydration technique of Vc two-step fermentation ultrafiltration waste slag liquid |
| CN104710232B (en) * | 2013-12-16 | 2018-06-08 | 中国科学院沈阳应用生态研究所 | One kind discards culture medium and fermented waste of vitamin C gulonic acid mother solution production organic fertilizer and preparation method thereof with Cordceps militaris |
| CN104710232A (en) * | 2013-12-16 | 2015-06-17 | 中国科学院沈阳应用生态研究所 | Organic fertilizer prepared by use of cordyceps militaris waste medium and vitamin C fermentation waste gulonic acid mother liquor and preparation method thereof |
| CN105175188A (en) * | 2015-09-24 | 2015-12-23 | 辽宁普天科技有限公司 | Method for preparing fulvic acid calcium magnesium fertilizers |
| CN105175188B (en) * | 2015-09-24 | 2018-12-28 | 辽宁普天科技有限公司 | A kind of preparation method of fulvic acid calcium and magnesium fertilizer |
| CN106010561A (en) * | 2016-06-08 | 2016-10-12 | 内蒙古阜丰生物科技有限公司 | Soil conditioning agent containing threonine fermentation waste |
| CN106010561B (en) * | 2016-06-08 | 2019-06-21 | 内蒙古阜丰生物科技有限公司 | A kind of soil conditioner containing Threonine Fermentation waste |
| CN106242680A (en) * | 2016-08-02 | 2016-12-21 | 李红旗 | Aminoacid water soluble fertilizer prepared by a kind of mother solution utilizing fermentative Production riboflavin and preparation method thereof |
| CN109265215A (en) * | 2017-07-17 | 2019-01-25 | 何立肖 | The processing method of gulonic acid mother solution in a kind of VC production |
| CN109354521A (en) * | 2018-09-26 | 2019-02-19 | 广东省农业科学院农业资源与环境研究所 | A kind of Amino acid liquid fertilizer mother liquor and preparation method thereof |
| CN112142510A (en) * | 2019-06-28 | 2020-12-29 | 沈阳颐康环境生物科技开发有限公司 | Regulating solution for improving vitamin C content of crops and preparation method and application thereof |
| CN110295206A (en) * | 2019-07-11 | 2019-10-01 | 沈阳中科微道生物科技有限公司 | A kind of method and its application using the fermented abandoned mash preparation association preparation of Vc |
| CN111000028A (en) * | 2019-12-30 | 2020-04-14 | 南通励成生物工程有限公司 | Animal feed and preparation method thereof |
| CN111995467A (en) * | 2020-09-08 | 2020-11-27 | 山东鲁维制药有限公司 | Method for producing mycoprotein and polypeptide organic fertilizer by using vitamin C fermentation waste |
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