CN104286383A - Tea seed meal detoxifying method - Google Patents
Tea seed meal detoxifying method Download PDFInfo
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- CN104286383A CN104286383A CN201410487785.XA CN201410487785A CN104286383A CN 104286383 A CN104286383 A CN 104286383A CN 201410487785 A CN201410487785 A CN 201410487785A CN 104286383 A CN104286383 A CN 104286383A
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- tea oil
- aspergillus niger
- lactococcus lactis
- oil slag
- leached tea
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Abstract
The invention discloses a tea seed meal detoxifying method. The tea seed meal detoxifying method comprises the step of mixing and fermenting tea seed meal raw materials by using Aspergillus niger and Lactococcus lactis. The method comprises the following specific steps: adding water which is 3-5 times heavier than tea seed meal into the tea seed meal and stirring to be uniform; treating at 60-90 DEG C for 1-3 hours; respectively cultivating Aspergillus niger strains and Lactococcus lactis strains; adjusting the pH of the treated tea seed meal to 6.5-7.5; adding cane molasses; carrying out heat treatment at 60-90 DEG C for one hour and inoculating; and fermenting for 72 hours, and drying and crushing a fermented product to obtain the detoxified tea seed meal. According to the tea seed meal detoxifying method, the Aspergillus niger strains and the Lactococcus lactis strains are used for fermenting and have an excellent synergic effect; and after the fermentation, the content of tea saponin is remarkably reduced and the content of small molecule peptides is remarkably improved.
Description
Technical field
The present invention relates to biological technical field, particularly relate to a kind of leached tea oil slag poison-removing method.
Background technology
Oil tea is the distinctive a kind of pure natural special oil tannin plant of China, accounts for more than 80% of national woody edible oil material.China's oil tea area about has fifty-five million mu, and tea seed annual production is about 1,000,000 tons, and produce tea oil per year up to 200,000 tons, estimating the output value is 11,000,000,000 yuan.According to oil tea tea seed oil content 26% ~ 39%, oil yield 70% calculates, camellia seed meal annual production about 700,000 tons, and this year, tea oil output increased year by year, causes leached tea oil slag output also to enter growth fast traffic lane.
The ANFs such as Tea Saponin, polyphenols too high levels in the leached tea oil slag that tea oil produces in producing, be not suitable for being directly used in animal feed, major part is used as pool-cleaning agent, fertilizer, fuel, even discarded, on a small quantity for the extraction of Tea Saponin, cause the significant wastage of resource.Leached tea oil slag generally contains crude fat, the protein of 10% ~ 20%, crude fibre, the glucide of 30% ~ 60%, the NFE of 20% ~ 50% of 15% ~ 25% of 0.5% ~ 7%.Containing 18 seed amino acids in camellia seed meal protein, comprise 10 kinds of essential amino acids needed for growth of animals or poultry, wherein threonine, glutamic acid, histidine and arginine equal size comparatively horn of plenty.Meanwhile, mineral matter and trace elements of Ca, K, Mg, Fe, Mn equal size also comparatively horn of plenty.If the ANFs in removing camellia seed meal, it just can be made to become the higher feedstuff of a kind of nutritive value.
Tea saponin is the major toxicity material in leached tea oil slag, and its toxicity main manifestations is hemolytic.Hemolytic is that its aglycon cholesterine on red blood cell wall is combined and generates water-fast complex precipitate, destroys Premeabilisation of cells pressure, makes red blood cell generation disintegration, and then cause haemolysis.Saponin(e is relevant with sapogenin with or without hemolytic activity, and the connection between active strong and weak size with glycan molecule, glycan molecule, the substituting group on glycan molecule etc. are relevant, relevant with the concentration of saponin(e in addition.Therefore those skilled in the art be devoted to exploitation one can significantly reduce tea saponin content, improve the technology of leached tea oil slag animal feed quality.
Cane molasses is the juice from sugar cane will squeezed out in sugar industry, through heating, neutralization, precipitation, filter, for dense fluids remaining after the sugaring operation such as concentrated, crystallization, be commonly called as malt sugar, belong to liquid energy feed.Cane molasses can stimulate cud (first stomach of ruminant) movable, improves a poor appetite, thus increases forage and dry matter intakes amount and help digest.The delicious food of molasses makes the absorption of forage increase.Other distasteful composition tastes are covered by molasses, thus ensure that the absorption of important element.Cane molasses, as the natural byproduct in a kind of sugared industry, is good energy source and vital movement attemperator.
Summary of the invention
Because the above-mentioned defect of prior art, technical problem to be solved by this invention is to provide a kind of method of leached tea oil slag detoxification.
For achieving the above object, the invention provides a kind of leached tea oil slag poison-removing method, comprise step:
Use aspergillus niger (Aspergillus niger) and Lactococcus lactis (Lactococcus lactis) mixed culture fermentation leached tea oil slag raw material.
Preferably, the concrete steps of described method are:
(1) leached tea oil slag pretreatment
Add the water of 3-5 times of weight portion in leached tea oil slag, stir, 60 DEG C-90 DEG C process 1-3h;
(2) Spawn incubation
Cultivate aspergillus niger strain and Lactococcus lactis bacterial classification respectively;
(3) semisolid fermentation
Leached tea oil slag pH to 6.5-7.5 pretreated in regulating step (1), adds cane molasses, gained bacterial classification in inoculation step (2) after 60 DEG C-90 DEG C heat treatment 1h, fermentation 72h;
(4) drying and crushing
Namely detoxification leached tea oil slag is obtained by after step (3) tunning drying and crushing.
Preferably, in step (1), in leached tea oil slag preprocessing process, pH is 9-12; Be more preferably 10-11.
Preferably, in step (2), aspergillus niger strain condition of culture is: sucrose 2.5% (w/v), dusty yeast 0.3% (w/v), peptone 1.0% (w/v), K
2hPO
40.1% (w/v), MgSO47H
2o 0.01% (w/v), cultivates 1-2 days for 25 DEG C.
Preferably, in step (2), Lactococcus lactis Spawn incubation condition is: peptone 1.0% (w/v), beef extract 1.0% (w/v), yeast extract 0.5% (w/v), glucose 2% (w/v), K
2hPO
40.2% (w/v), MgSO
47H
2o 0.002% (w/v), 37 DEG C of Anaerobic culturel 12-24 hour.
Preferably, in step (3), aspergillus niger strain inoculum concentration is 5-10% (v/v).
Preferably, in step (3), Lactococcus lactis strain inoculation amount is 20-30% (v/v).
Preferably, in step (3), the addition of cane molasses is the 5-10% of leached tea oil slag quality.
Technique effect
Technical scheme of the present invention, due to the existence of Tea Saponin in leached tea oil slag, make leached tea oil slag viscosity larger, the present inventor is surprised to find that in research, high-temperature process leached tea oil slag in the basic conditions, the viscosity of leached tea oil slag raw material can greatly be reduced, follow-up semisolid fermentation can be carried out smoothly.
The present invention uses aspergillus niger strain and Lactococcus lactis strain fermentation, has excellent synergisticing performance, and after fermentation, tea saponin content reduces significantly, and small-molecular peptides content significantly improves.
The present invention uses cane molasses as the raw material of fermentation medium, not only cheap, and the nutritional need in fermenting can be met completely, be suitable as very much the carbon source of aspergillus niger strain and the mixed culture fermentation of Lactococcus lactis bacterial classification, without the need to adding other composition again, simplify zymotechnique.The result of study in early stage shows, mixed culture fermentation is carried out with aspergillus niger and Lactococcus lactis, use cane molasses as carbon source, in tunning, the content of small-molecular peptides ferments apparently higher than using glucose (exceeding about 20%) and sucrose (exceeding about 10%) as carbon source.
Detailed description of the invention
In an embodiment of the present invention, relevant data detects by the following method:
Crude protein content detection method reference: standard GB/T/T6432-94 " in feed crude protein determining method ";
Water-solubility protein detection method reference: agricultural industry criteria NY/T1205-2006 " mensuration of soybean water-soluble protein content " performs;
Small-molecular peptides detection method of content reference: perform according to light industry standard QB/T2653-2004 " soy peptide powder ";
Tea Saponin detection method of content reference: Zhong Shian etc., Tea Saponin [J] in rp-hplc determination tea seed cake, physical and chemical inspection, 2008;
Content of cellulose detection method reference: standard GB/T/T5009.10-23.
Aspergillus niger (Aspergillus niger) and Lactococcus lactis (Lactococcus lactis) are purchased from Beijing North Na Kaichuan Bioisystech Co., Ltd.
Embodiment 1
(1) leached tea oil slag pretreatment
Add the water of 20 kilograms in 5 kilograms of leached tea oil slags, stir, regulate pH to be 10,90 DEG C of process 1h;
(2) Spawn incubation
The cultivation of aspergillus niger strain:
Sucrose 2.5% (w/v), dusty yeast 0.3% (w/v), peptone 1.0% (w/v), K
2hPO
40.1% (w/v), MgSO47H
2o 0.01% (w/v), cultivates 2 days for 25 DEG C.
Lactococcus lactis is cultivated:
Peptone 1.0% (w/v), beef extract 1.0% (w/v), yeast extract 0.5% (w/v), glucose 2% (w/v), K
2hPO
40.2% (w/v), MgSO
47H
2o 0.002% (w/v), 37 DEG C of Anaerobic culturel 24 hours.
(3) semisolid fermentation
Leached tea oil slag pH to 6.5 pretreated in regulating step (1), adds cane molasses 500g, inoculated aspergillus niger strain liquid 2.5L after 90 DEG C of heat treatment 1h, Lactococcus lactis strain liquid 5L, fermentation 72h;
(4) drying and crushing
Namely detoxification leached tea oil slag is obtained by after step (3) tunning drying and crushing.
With unfermentable leached tea oil slag for contrast, detect, in setting control group, projects content is 100%, and testing result is as shown in table 1.
Table 1
| Project | Embodiment 1 (%) | Contrast (%) |
| Crude protein | 156 | 100 |
| Water-solubility protein | 142 | 100 |
| Small-molecular peptides | 330 | 100 |
| Tea Saponin | 13 | 100 |
| Cellulose | 62 | 100 |
In experiment, inventor finds, if without pre-treatment step, is directly fermented by leached tea oil slag, and not only viscosity is comparatively large, and microscopy finds that Lactococcus lactis is difficult to growth, and the relative amount of tunning small molecular peptide is only about 160%.Through lot of experiments, applicant finally finds, in the basic conditions, after heat treatment leached tea oil slag, then ferments, and aspergillus niger and Lactococcus lactis all can normal growths, and the indices of tunning all has great improvement.
Embodiment 2
(1) leached tea oil slag pretreatment
Add the water of 15 kilograms in 5 kilograms of leached tea oil slags, stir, regulate pH to be 11,70 DEG C of process 2h;
(2) Spawn incubation
The cultivation of aspergillus niger strain:
Sucrose 2.5% (w/v), dusty yeast 0.3% (w/v), peptone 1.0% (w/v), K
2hPO
40.1% (w/v), MgSO47H
2o 0.01% (w/v), cultivates 2 days for 25 DEG C.
Lactococcus lactis is cultivated:
Peptone 1.0% (w/v), beef extract 1.0% (w/v), yeast extract 0.5% (w/v), glucose 2% (w/v), K
2hPO
40.2% (w/v), MgSO
47H
2o 0.002% (w/v), 37 DEG C of Anaerobic culturel 24 hours.
(3) semisolid fermentation
Leached tea oil slag pH to 7.5 pretreated in regulating step (1), adds cane molasses 1000g, inoculated aspergillus niger strain liquid 4L after 90 DEG C of heat treatment 1h, Lactococcus lactis strain liquid 4L, fermentation 60h;
(4) drying and crushing
Namely detoxification leached tea oil slag is obtained by after step (3) tunning drying and crushing.
With unfermentable leached tea oil slag for contrast, detect, in setting control group, projects content is 100%, and testing result is as shown in table 2.
Table 2
| Project | Embodiment 2 (%) | Contrast (%) |
| Crude protein | 143 | 100 |
| Water-solubility protein | 130 | 100 |
| Small-molecular peptides | 221 | 100 |
| Tea Saponin | 8 | 100 |
| Cellulose | 66 | 100 |
In the present embodiment, aspergillus niger strain liquid inoculum concentration is larger, relative Lactococcus lactis strain liquid inoculum concentration is less, as can be seen from the result of table 2, in tunning, albumen index (crude protein, water-solubility protein, small-molecular peptides) is not as embodiment 1, but Tea Saponin surplus index is better than embodiment 1, the degraded strengthening aspergillus niger inoculum concentration and be conducive to Tea Saponin is described.
Embodiment 3
(1) leached tea oil slag pretreatment
Add the water of 25 kilograms in 5 kilograms of leached tea oil slags, stir, regulate pH to be 9,80 DEG C of process 1h;
(2) Spawn incubation
The cultivation of aspergillus niger strain:
Sucrose 2.5% (w/v), dusty yeast 0.3% (w/v), peptone 1.0% (w/v), K
2hPO
40.1% (w/v), MgSO47H
2o 0.01% (w/v), cultivates 2 days for 25 DEG C.
Lactococcus lactis is cultivated:
Peptone 1.0% (w/v), beef extract 1.0% (w/v), yeast extract 0.5% (w/v), glucose 2% (w/v), K
2hPO
40.2% (w/v), MgSO
47H
2o 0.002% (w/v), 37 DEG C of Anaerobic culturel 24 hours.
(3) semisolid fermentation
Leached tea oil slag pH to 7.0 pretreated in regulating step (1), adds cane molasses 1300g, inoculated aspergillus niger strain liquid 3L after 80 DEG C of heat treatment 1h, Lactococcus lactis strain liquid 5L, fermentation 60h;
(4) drying and crushing
Namely detoxification leached tea oil slag is obtained by after step (3) tunning drying and crushing.
With unfermentable leached tea oil slag for contrast, detect, in setting control group, projects content is 100%, and testing result is as shown in table 3.
Table 3
| Project | Embodiment 3 (%) | Contrast (%) |
| Crude protein | 126 | 100 |
| Water-solubility protein | 143 | 100 |
| Small-molecular peptides | 362 | 100 |
| Tea Saponin | 16 | 100 |
| Cellulose | 73 | 100 |
In the present embodiment, aspergillus niger strain liquid inoculum concentration is less, relative Lactococcus lactis strain liquid inoculum concentration is larger, as can be seen from the result of table 3, in tunning, albumen index (crude protein, water-solubility protein, small-molecular peptides) is better, the inoculum concentration strengthening Lactococcus lactis bacterial classification is described, is conducive to the generation of leached tea oil slag small molecular peptide.
Comparative example 1
In this comparative example, only inoculated aspergillus niger or Lactococcus lactis ferment, and other conditions are with embodiment 1, and fermentation results is as shown in table 4.
Table 4
| Project | Only inoculated aspergillus niger (%) | Only inoculating lactic acid galactococcus (%) | Contrast (%) |
| Crude protein | 121 | 138 | 100 |
| Water-solubility protein | 108 | 112 | 100 |
| Small-molecular peptides | 132 | 183 | 100 |
| Tea Saponin | 18 | 32 | 100 |
| Cellulose | 76 | 86 | 100 |
As can be seen from the above results, only use aspergillus niger or Lactococcus lactis to ferment, ferment effect is obviously not as the mixed culture fermentation of two kinds of bacterial strains.
More than describe preferred embodiment of the present invention in detail.Should be appreciated that those of ordinary skill in the art just design according to the present invention can make many modifications and variations without the need to creative work.Therefore, all technical staff in the art, all should by the determined protection domain of claims under this invention's idea on the basis of existing technology by the available technical scheme of logical analysis, reasoning, or a limited experiment.
Claims (8)
1. a leached tea oil slag poison-removing method, is characterized in that, comprises step: use aspergillus niger (Aspergillus niger) and Lactococcus lactis (Lactococcus lactis) mixed culture fermentation leached tea oil slag raw material.
2. the method for claim 1, wherein the concrete steps of described method are:
(1) leached tea oil slag pretreatment
Add the water of 3-5 times of weight portion in leached tea oil slag, stir, 60 DEG C-90 DEG C process 1-3h;
(2) Spawn incubation
Cultivate aspergillus niger strain and Lactococcus lactis bacterial classification respectively;
(3) semisolid fermentation
Leached tea oil slag pH to 6.5-7.5 pretreated in regulating step (1), adds cane molasses, gained bacterial classification in inoculation step (2) after 60 DEG C-90 DEG C heat treatment 1h, fermentation 72h;
(4) drying and crushing
Namely detoxification leached tea oil slag is obtained by after step (3) tunning drying and crushing.
3., the method for claim 1, wherein in step (1), in leached tea oil slag preprocessing process, pH is 9-12; Be more preferably 10-11.
4., the method for claim 1, wherein in step (2), aspergillus niger strain condition of culture is: sucrose 2.5% (w/v), dusty yeast 0.3% (w/v), peptone 1.0% (w/v), K
2hPO
40.1% (w/v), MgSO47H
2o 0.01% (w/v), cultivates 1-2 days for 25 DEG C.
5. the method for claim 1, wherein, in step (2), Lactococcus lactis Spawn incubation condition is: peptone 1.0% (w/v), beef extract 1.0% (w/v), yeast extract 0.5% (w/v), glucose 2% (w/v), K
2hPO
40.2% (w/v), MgSO
47H
2o 0.002% (w/v), 37 DEG C of Anaerobic culturel 12-24 hour.
6., the method for claim 1, wherein in step (3), aspergillus niger strain inoculum concentration is 5-10% (v/v).
7., the method for claim 1, wherein in step (3), Lactococcus lactis strain inoculation amount is 20-30% (v/v).
8., the method for claim 1, wherein in step (3), the addition of cane molasses is the 5-10% of leached tea oil slag quality.
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|---|---|---|---|
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028920A (en) * | 2015-08-05 | 2015-11-11 | 安徽大别山科技开发有限公司 | High-quality tea dreg used for feed adding and preparing method thereof |
| CN108977367A (en) * | 2018-08-28 | 2018-12-11 | 中南林业科技大学 | One plant of Aspergillus niger strain and its degradation cake of camellia oleifera seeds in Tea Saponin application |
| CN110384183A (en) * | 2019-09-02 | 2019-10-29 | 佛山播恩生物科技有限公司 | A kind of fermentation leached tea oil slag biological feedstuff and preparation method thereof |
| CN113604508A (en) * | 2021-09-01 | 2021-11-05 | 安徽农业大学 | Fermentation improvement method of peony seed meal for feed, fermentation product and application thereof |
-
2014
- 2014-09-16 CN CN201410487785.XA patent/CN104286383A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105028920A (en) * | 2015-08-05 | 2015-11-11 | 安徽大别山科技开发有限公司 | High-quality tea dreg used for feed adding and preparing method thereof |
| CN108977367A (en) * | 2018-08-28 | 2018-12-11 | 中南林业科技大学 | One plant of Aspergillus niger strain and its degradation cake of camellia oleifera seeds in Tea Saponin application |
| CN108977367B (en) * | 2018-08-28 | 2022-04-15 | 中南林业科技大学 | Aspergillus niger strain and application thereof in degradation of tea saponin in camellia oleifera cake |
| CN110384183A (en) * | 2019-09-02 | 2019-10-29 | 佛山播恩生物科技有限公司 | A kind of fermentation leached tea oil slag biological feedstuff and preparation method thereof |
| CN113604508A (en) * | 2021-09-01 | 2021-11-05 | 安徽农业大学 | Fermentation improvement method of peony seed meal for feed, fermentation product and application thereof |
| CN113604508B (en) * | 2021-09-01 | 2024-02-02 | 安徽农业大学 | Fermentation improvement method of feeding peony seed meal, fermentation product and application thereof |
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