CN105062493A - Preparation method of microbial soil amendment - Google Patents
Preparation method of microbial soil amendment Download PDFInfo
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- CN105062493A CN105062493A CN201510454635.3A CN201510454635A CN105062493A CN 105062493 A CN105062493 A CN 105062493A CN 201510454635 A CN201510454635 A CN 201510454635A CN 105062493 A CN105062493 A CN 105062493A
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Abstract
The invention discloses a preparation method of a microbial soil amendment. The preparation method comprises the following steps: inoculating lactobacillus acidophilus, streptococcus faecium, candida utilis, lactobacillus planetarium, and rhodopseudomonas palustris into an anaerobic medium, a light irritation medium, and an aerobic medium respectively, culturing, when the cell concentration of each bacterium seed solution reaches 106/mL, inoculating the bacteria into a mixed medium, mixing, and then carrying out anaerobic fermentation. According to the preparation method, several safe and beneficial bacterium strains are chosen; according to the biological characters of the bacterium strains, the bacterium strains are reasonably compounded so as to obtain a mixed bacterium group for fermentation. The medium is cheap and pertinent and can provide proper culture/fermentation conditions for different bacteria. The prepared microbial soil amendment can improve the soil, degrade and convert residual substances of continuous cropping in the soil, enhance the urease activity, and improve the soil fertility. Moreover, the water retaining performance and breathability of soil are improved, the soil's physical and chemical properties are also improved, the soil microecological environment is balanced, and a beneficial soil microecological environment is established.
Description
Technical field
The present invention relates to a kind of preparation method of microbe soil conditioner.
Background technology
Over nearly 20 years, China's soil fertility sharply declines, and soil ecology worsens, and heavy metal severe overweight, has a strong impact on the health of our people and the Sustainable development of economy.Microbial technique has become with its safety, feature that is efficient, environmental protection the important channel solving China's soil issues.
Summary of the invention
Object of the present invention is just to provide a kind of preparation method of microbe soil conditioner, and prepared microbe soil conditioner can be increase soil fertility, decompose soil organic substance, balanced soil Tiny ecosystem relation.
The preparation method of microbe soil conditioner of the present invention, comprises the following steps:
1, Lactobacterium acidophilum, streptococcus faecium, plant lactobacillus are linked into row Anaerobic culturel in anaerobic culture medium with the ratio of 1% respectively, the weight percent of this Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract paste: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Be linked into producing the ratio of Ruan's candidiasis with 1% in aerobic culture medium oxygen cultivation of acting charitably, the weight percent of this aerobic culture medium raw material is, water: molasses: urea: yeast extract paste=60%:35%:4.5%:0.5%; By Rhodopseudomonas palustris with 1% ratio be linked into the capable illumination cultivation of photosynthetic bacteria culture medium, illuminance 3000-4000LX, the weight percent of this photosynthetic bacteria culture medium raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract paste: potassium primary phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
2, when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with after mixing in the ratio access mixed culture medium of 1%, in loading mash-back, anaerobically fermenting 20 days, stops fermentation to pH value 3-4, the weight percent of described mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%;
3, filtration, clarification get product.
The preparation method of microbe soil conditioner of the present invention, screened several safety, meet national relevant laws and regulations, the useful bacterial strain of tool improvement ecological environment of soil effect, according to the biological characteristics of each bacterial strain, reasonably combined, be combined into fermentation composite flora; Substratum is with strong points, cheap and easy to get; According to the physiological ecological habit of different strain, provide the cultivation and fermentation condition being suitable for different strain.
The preparation method of microbe soil conditioner of the present invention, prepared microbe soil conditioner has following functions:
1, improve soil, eliminate soil continuous cropping obstacle, continuous cropping residuals in decomposition and inversion soil, strengthen soil urease liveness, increase soil fertility;
2, decompose soil organic substance, improve soil water-retaining, permeability, improvement soil physical and chemical property;
3, suppress harmful miscellaneous bacteria propagation, balanced soil Tiny ecosystem relation, builds useful soil microenvironment;
4, change residual heavy metal valence state in soil, make it be converted into nontoxic material.
Embodiment
A preparation method for microbe soil conditioner, comprises the following steps:
1, Lactobacterium acidophilum, streptococcus faecium, plant lactobacillus are linked into row Anaerobic culturel in anaerobic culture medium with the ratio of 1% respectively, the weight percent of this Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract paste: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Be linked into producing the ratio of Ruan's candidiasis with 1% in aerobic culture medium oxygen cultivation of acting charitably, the weight percent of this aerobic culture medium raw material is, water: molasses: urea: yeast extract paste=60%:35%:4.5%:0.5%; By Rhodopseudomonas palustris with 1% ratio be linked into the capable illumination cultivation of photosynthetic bacteria culture medium, illuminance 4000LX, the weight percent of this photosynthetic bacteria culture medium raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract paste: potassium primary phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
2, when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with after mixing in the ratio access mixed culture medium of 1%, in loading mash-back, anaerobically fermenting 20 days, stops fermentation to pH value 3.6, the weight percent of described mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%;
3, filtration, clarification get product.
Claims (1)
1. a preparation method for microbe soil conditioner, is characterized in that: it comprises the following steps:
(1), Lactobacterium acidophilum, streptococcus faecium, plant lactobacillus are linked into row Anaerobic culturel in anaerobic culture medium with the ratio of 1% respectively, the weight percent of this Anaerobic culturel based raw material is, molasses: water: dipotassium hydrogen phosphate: yeast extract paste: dregs of beans=35%:60%:0.1%:0.4%:4.5%; Be linked into producing the ratio of Ruan's candidiasis with 1% in aerobic culture medium oxygen cultivation of acting charitably, the weight percent of this aerobic culture medium raw material is, water: molasses: urea: yeast extract paste=60%:35%:4.5%:0.5%; By Rhodopseudomonas palustris with 1% ratio be linked into the capable illumination cultivation of photosynthetic bacteria culture medium, illuminance 3000-4000LX, the weight percent of this photosynthetic bacteria culture medium raw material is, water: molasses: dipotassium hydrogen phosphate: yeast extract paste: potassium primary phosphate: calcium chloride: magnesium chloride: ammonium chloride=60%:35%:0.5%:1%:0.5%:1%:1%:1%;
(2), when above-mentioned each bacterial classification seed liquor cell concentration reaches 10
6during/ml, respectively with after mixing in the ratio access mixed culture medium of 1%, in loading mash-back, anaerobically fermenting 20 days, stops fermentation to pH value 3-4, the weight percent of described mixed culture based raw material is, molasses: water: dipotassium hydrogen phosphate: magnesium sulfate=35%:63%:0.5%:1.5%;
(3), filtration, clarification get product.
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| CN201510454635.3A CN105062493A (en) | 2015-07-30 | 2015-07-30 | Preparation method of microbial soil amendment |
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| CN201510454635.3A CN105062493A (en) | 2015-07-30 | 2015-07-30 | Preparation method of microbial soil amendment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828696A (en) * | 2017-11-30 | 2018-03-23 | 盖萌 | A kind of composite fermentation microbe soil conditioner and preparation method thereof |
| CN114480203A (en) * | 2022-02-18 | 2022-05-13 | 北京意科乐生态科技有限公司 | Composite microbial preparation for resisting continuous cropping obstacles of Chinese herbal medicines and application method |
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| CN102249752A (en) * | 2011-04-15 | 2011-11-23 | 北京康源三色生物科技有限公司 | Microbial fertilizer and preparation method and application thereof |
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2015
- 2015-07-30 CN CN201510454635.3A patent/CN105062493A/en active Pending
Patent Citations (5)
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| CN101486969A (en) * | 2009-02-18 | 2009-07-22 | 新疆农业科学院土壤肥料与农业节水研究所 | Preparation of compost fermentation complex bacterial agent |
| CN101974428A (en) * | 2010-09-30 | 2011-02-16 | 北京意科乐生态科技有限公司 | Complex microbial preparation capable of resisting replant obstacle resistance and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107828696A (en) * | 2017-11-30 | 2018-03-23 | 盖萌 | A kind of composite fermentation microbe soil conditioner and preparation method thereof |
| CN114480203A (en) * | 2022-02-18 | 2022-05-13 | 北京意科乐生态科技有限公司 | Composite microbial preparation for resisting continuous cropping obstacles of Chinese herbal medicines and application method |
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Application publication date: 20151118 |