CN101899397B - Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application - Google Patents
Composite efficient microbial preparation for treating difficultly-degradable waste water and preparation and application Download PDFInfo
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Abstract
The invention discloses a composite efficient microbial preparation for treating difficultly-degradable waste water. The composite efficient microbial preparation comprises the following active ingredients of: bacillus, pseudomonas, alcaligenes eutrophus, aspergillus and yeast. The composite efficient microbial preparation has a unique treating effect on high-concentration organic sewage and high ammonia and nitrogen sewage which are difficult to treat by the conventional activated sludge process and contain macromolecules and difficultly-degradable harmful constituents.
Description
Technical field
The present invention relates to a kind of composite and highly effective microorganism preparation and preparation and application that used water difficult to degradate is handled that be used for.
Background technology
The biologic treating technique of waste water is the dominant technology of current field of waste water treatment, and it is improving the water body environment quality, preventing bringing into play important effect aspect the water pollution as a kind of economy, reasonable, effective wastewater processing technology.
The biological treatment of waste water is the metabolism that utilizes mikrobe, and the decomposition and inversion pollutants in waste water reaches the purpose that purifies water.Its core is to cultivate the superior microorganism flora that domestication is fit to waste water characteristic; And maintenance and contaminated wastewater the substrate concentration microbial biomass and the activity that adapt; The superior microorganism flora is handled in best degrading activity; Give full play to its degradation function, improve the biological treatment ability and the processing efficiency of system to greatest extent.
Nature culturing sludge acclimated microorganism is generally adopted in the biological treatment of waste water at present, and active sludge has been brought into play important role as traditional microorganism culturing domestication mode in field of waste water treatment especially municipal wastewater process field.But; In recent decades because the continuous development of industry; The continuous use of new technology, novel material; Make the pollutent in the environment become complicated more; Particularly a large amount of artificial-synthetic compounds get into environment, and this type material mainly is waste water or the solid useless environment that gets into along with factory's discharging such as the organism of alkanes, olefines, alicyclic hydrocarbon type, arene, synthetic, especially macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene,toluene,xylene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), cyanogen class, amine and ammonia nitrogen; Because complicacy of these these body structures of material and biological strange property; At short notice can not be by the microbiological degradation utilization, traditional method of wastewater treatment is cultivated acclimated microorganism with active sludge and can not be removed these pollutents effectively, and these materials accumulate in environment for a long time; The ecotope of depending on for existence to us causes very big pollution, brings very big harm for human physical and mental health.In addition, because the continuous development of urban construction, urban land is nervous year by year, and the traditional activated sludge process processing technological flow is long, and floor space is big, and contradictions such as complicated operation are also outstanding day by day.And can bring into play the potentiality of mikrobe well to the special composite and highly effective microorganism preparation of preparing of the pollutent of these difficult degradations; Can effectively degrade and remove these difficult degradations, poisonous and hazardous pollutent; Purify our ecotope, effectively address these problems and contradiction.
Composite and highly effective microorganism preparation is exactly the efficient bacterial strain that filters out from nature to different difficult degradation pollutent or the engineering bacteria of cultivating domestication through the biotechnology means; According to special formula,, be mixed with high-efficiency microorganism preparation with the various bacterial classifications that can form dominant microflora; Be added in the Waste Water Treatment by a certain amount of; Can cultivate at short notice and tame out the dominant microflora that is fit to waste water characteristic, improve microbic activity and quantity greatly, quicken the degraded of mikrobe pollutent; To improve the biological treatment efficient of system, guarantee system stable operation.So the high-effective microorganism Study on Technology is rapid with the application development in recent years, has broad application prospects.
Summary of the invention
In order to solve the problems of the technologies described above, the invention provides a kind of active high, degradation capability is strong, processing efficiency is high, to composite and highly effective microorganism preparation and the preparation and the application that used water difficult to degradate is handled that be used for of difficult degradation, hazardous and noxious substances and ammonia nitrogen degradation ability and strong shock resistance.Above-mentioned used water difficult to degradate is meant the organism that contains alkanes, olefines, alicyclic hydrocarbon type, arene, synthetic, especially the waste water of macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene,toluene,xylene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), cyanogen class, amine and ammonia nitrogen.
The activeconstituents of composite and highly effective microorganism preparation provided by the invention comprises: genus bacillus, pseudomonas, Alcaligenes, aspergillus and yeast.
As preferably; Above-mentioned genus bacillus is a kind of bacterial classification of bacillus or the arbitrary combination of two or more bacterial classifications; Said pseudomonas is a kind of bacterial classification of Rhodopseudomonas or the arbitrary combination of two or more bacterial classifications; Above-mentioned Alcaligenes is a kind of or its combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, and above-mentioned aspergillus is a kind of or its combination in black mold and the aspergillus oryzae, and above-mentioned yeast is a kind of or its combination in yeast saccharomyces cerevisiae and the Candida utilis.
As preferably, above-mentioned genus bacillus is subtilis, bacillus amyloliquefaciens, separate one or more arbitrary combination in sugared clostridium spp and the Bacillus sphaericus; Above-mentioned pseudomonas is a kind of in Pseudomonas fluorescens, Pseudomonas aeruginosa, Pseudomonas alcaligenes and the denitrifying pseudomonas or combination that they are two or more.
As preferably, the mentioned microorganism composite fungus agent is made up of the culture of bacterium and fungi, comprises following compositions in portion by weight:
Bacillus culture 25~35,
Rhodopseudomonas culture 15~25,
Alkaligenes culture 10~20,
Aspergillus culture 10~20,
Saccharomycodes culture 10~20,
Wherein, said bacillus culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the bacillus, and wherein the content of genus bacillus is hundred million of 50-100/gram; Said Rhodopseudomonas culture is the culture of a kind of bacterial classification or two or more bacterial classification combination in the Rhodopseudomonas, and wherein the content of pseudomonas is hundred million of 50-100/gram; Said Alkaligenes culture is the culture of a kind of or its combination in Bacillus foecalis alkaligenes and the denitration Alcaligenes, and wherein the content of Alcaligenes is hundred million of 50-100/gram; Said Aspergillus culture is the culture of a kind of or its combination in black mold and the aspergillus oryzae, wherein hundred million/gram of aspergillar content 10-50; Said Saccharomycodes culture is the culture of a kind of or its combination in yeast saccharomyces cerevisiae and the Candida utilis, and wherein saccharomycetic content is hundred million of 10-50/gram.
The present invention provides a kind of method for preparing above-mentioned composite and highly effective microorganism preparation, comprises the steps:
1) from bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus and Saccharomycodes, respectively selects one or more bacterial classifications, carry out seed enlarged culturing step by step respectively, obtain the kind daughter bacteria liquid of enlarged culturing;
The kind daughter bacteria liquid of the enlarged culturing that 2) step 1) is obtained inserts fermentor tank respectively; Inoculum size by weight or volumeter; Be the 5-10% of fermention medium, different Pseudomonas carries out fermentation culture respectively separately, and the bacterial classification more than 2 or 2 of same Pseudomonas carries out mixing fermentation culture;
3) fermention medium initial p H is adjusted to 6.0-8.0, and controlled temperature 30-40 ℃, fermentation time 24-48 hour, fermenting process adopted mechanical stirring or feeds the filtrated air oxygen supply;
4) with bacillus culture, Rhodopseudomonas culture, Alkaligenes culture, Aspergillus culture and the Saccharomycodes culture of said amount, mix, obtain composite and highly effective microorganism preparation, preserve.
As preferably, step 2) said fermention medium is a liquid nutrient medium, contains the aqueous solution of following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, an ammonium nitrate 1500-2000
Mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03mg/L.The said composite and highly effective microorganism preparation of step 4) is the liquid composite and highly effective microorganism preparation, preserves with canning sealing.
As preferably; Step 2) said fermention medium is made up of 1: 1 solid medium of weight ratio and liquid nutrient medium; Wherein, Said solid medium is made up of following ingredients by weight percentage: wheat bran 80-90%, Semen Maydis powder 5-10% and soybean cake powder 5-10%, said liquid nutrient medium are the aqueous solution that contains following concentration composition: glucose 40000-60000mg/L, saltpetre 1500-2500mg/L, an ammonium nitrate 1500-2000mg/L, calcium chloride 400-500mg/L, sal epsom 450-500mg/L, potassium primary phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganous sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, potassiumiodide 0.5-1.0mg/L, Sodium orthomolybdate 0.2-0.3mg/L and copper sulfate 0.02-0.03
mg/L。The said composite and highly effective microorganism preparation of step 4) is the solid composite and highly effective microorganism preparation, the oven dry packed preservation in back under 40-45 ℃ of temperature.
The present invention also provides the application of above-mentioned composite and highly effective microorganism preparation in used water difficult to degradate is handled, and above-mentioned composite and highly effective microorganism preparation is added in the wastewater treatment biochemistry pool.Addition method and dosage are: activated sludge process adds by the biochemistry pool volume, and every cubic metre of biochemistry pool volume adds above-mentioned composite and highly effective microorganism preparation 20-30 gram at every turn, adds every day 1 time, adds continuously 10-15 days; Biomembrance process adds by the volume of filler, and every cubic metre of packing volume adds above-mentioned composite and highly effective microorganism preparation 30-50 gram at every turn, adds every day 1 time, adds continuously 10-15 days.
The present invention can reach following technique effect:
Composite and highly effective microorganism preparation of the present invention contains the multiple mikrobe that the difficult degradation pollutent is had good degradation capability; Active high; Living weight is big; Breeding is fast; Add in Waste Water Treatment, it is short to cultivate acclimation period, can in 10-30 days, fast culture tame out the superior microorganism flora that is fit to waste water characteristic; Especially macromole, difficult degradation, hazardous and noxious substances such as phenols (phenol, chlorophenol, cresols, nitrophenol etc.), arene (benzene,toluene,xylene, oil of mirbane, phenol type substances, naphthalene, anthracene, quinone etc.), cyanogen class, amine and ammonia nitrogen, phosphorus etc. there are good degradation effect, reluctant high concentration organic sewage of traditional activated sludge process and high-ammonia-nitrogen sewage are had unique treatment effect.Be particularly suitable for the cultivation domestication of newly-built industrial sewage treatment plant superior microorganism and the dilatation and the technological transformation of old sewage work, can improve and handle the water yield and water quality treatment, reduce working cost, the assurance qualified discharge.Be simple, economic, the effective modern technique that quick startup of new system and old sewage work carry out extending capacity reformation, can practice thrift investment cost greatly, obtain the optimum handling effect.
Embodiment
Below in conjunction with specific embodiment the present invention is described further so that those skilled in the art can better understand the present invention and implementing, but the embodiment that lifts not conduct to qualification of the present invention.
Embodiment 1:
1, selects subtilis, Pseudomonas fluorescens, Bacillus foecalis alkaligenes, black mold and yeast saccharomyces cerevisiae, above-mentioned bacterial classification is carried out seed enlarged culturing step by step respectively.
2, add the clean tap water of 5000mL in the container after disinfects; Add glucose 200g, saltpetre 7.5g, an ammonium nitrate 7.5g, calcium chloride 2.0g, sal epsom 2.25g, potassium primary phosphate 750mg, inositol 750mg, ferrous sulfate 125mg, manganous sulfate 100mg, zinc sulfate 25mg, boric acid 25mg, potassiumiodide 2.5mg, Sodium orthomolybdate 1.0mg, copper sulfate 0.1mg respectively; Fully stirring and dissolving, mix, regulate PH to 6.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 1000mL.
3, in each beaker, inoculate the kind liquid 100mL of subtilis, Pseudomonas fluorescens, Bacillus foecalis alkaligenes, black mold and yeast saccharomyces cerevisiae enlarged culturing successively; Put into 30 ℃ thermostat container fermentation culture 24 hours after stirring; Whenever stirred 1 time at a distance from 2 hours between yeast phase; Control PH in the fermenting process to 6.0-8.0, after fermentation was accomplished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
4, by weight with cultured fermented product; Press following mixed: 25 parts of Bacillus subtillis cultures, 25 parts of Pseudomonas fluorescens cultures, 20 parts of Bacillus foecalis alkaligenes cultures, 10 parts of black mold cultures; 10 parts of yeast saccharomyces cerevisiae cultures mix the back canning sealing.
Embodiment 2:
1, choose shuttles shape genus bacillus, Pseudomonas alcaligenes, denitration Alcaligenes, aspergillus oryzae and Candida utilis bacterium are with above-mentioned bacterial classification, carry out seed enlarged culturing step by step respectively.
2, the clean tap water that adds 2500mL in the container after disinfects; Add glucose 150g, saltpetre 6.25g, an ammonium nitrate 5g, calcium chloride 1.25g, sal epsom 1.25g, potassium primary phosphate 500mg, inositol 625mg, ferrous sulfate 87.5mg, manganous sulfate 62 respectively; 5mg, zinc sulfate 25mg, boric acid 25mg, potassiumiodide 2.5mg, Sodium orthomolybdate 0.75mg, copper sulfate 0.075mg; Fully stirring and dissolving, mix, regulate PH to 8.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 500mL.
3, in the beaker of above-mentioned 5 nutrient solutions, add wheat bran 400g, Semen Maydis powder 50g, soybean cake powder 50g after sterilising treatment respectively, mixing and stirring.
4, in each beaker, inoculate the kind liquid 50mL of Bacillus sphaericus, Pseudomonas alcaligenes, denitration Alcaligenes, aspergillus oryzae and Candida utilis bacterium enlarged culturing successively; Put into 40 ℃ thermostat container fermentation culture 48 hours after stirring; Whenever stirred 1 time at a distance from 2 hours between yeast phase; Control PH in the fermenting process to 6.0-8.0, after fermentation was accomplished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
5, by weight with cultured fermented product; Press following mixed: 35 parts of Bacillus sphaericus cultures, 15 parts of Pseudomonas alcaligenes cultures, 10 parts of denitration Alcaligenes cultures, 20 parts of aspergillus oryzae cultures, 20 parts of Candida utilis bacterium cultures.It is packed to mix oven dry back under 40-45 ℃ of temperature, back.
Embodiment 3:
1, selects the bacillus amyloliquefaciens of bacillus to conciliate Pseudomonas aeruginosa and the denitrifying pseudomonas of sugared clostridium spp, Rhodopseudomonas, Bacillus foecalis alkaligenes and denitration Alcaligenes, the black mold of Aspergillus and the yeast saccharomyces cerevisiae and the Candida utilis of aspergillus oryzae and Saccharomycodes of Alkaligenes, above-mentioned bacterial classification, each bacterial classification are carried out seed enlarged culturing step by step respectively.
2, add the clean tap water of 2500mL in the container after disinfects; Add glucose 125g, saltpetre 5g, an ammonium nitrate 4.5g, calcium chloride 1.2g, sal epsom 1.2g, potassium primary phosphate 450mg, inositol 500mg, ferrous sulfate 75mg, manganous sulfate 55mg, zinc sulfate 20mg, boric acid 20mg, potassiumiodide 2mg, Sodium orthomolybdate 0.7mg, copper sulfate 0.07mg respectively; Fully stirring and dissolving, mix, regulate PH to 7.0.Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 500mL.
3, in the beaker of above-mentioned 5 nutrient solutions, add wheat bran 450g, Semen Maydis powder 25g, soybean cake powder 25g after sterilising treatment respectively, mixing and stirring.
4, in each beaker, inoculate the kind liquid of bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus, Saccharomycodes enlarged culturing successively; The kind liquid of two strain expanded culture in each Pseudomonas meets 50mL respectively; Put into 35 ℃ thermostat container fermentation culture 36 hours after stirring; Whenever stirred 1 time at a distance from 2 hours between yeast phase; Control PH 6.0-8.0 in the fermenting process, after fermentation was accomplished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram.
5, by weight with cultured fermented product; Press following mixed: 30 parts of Bacillus cultures, 20 parts of Rhodopseudomonas cultures, 15 parts of Alkaligenes cultures, 17 parts of Aspergillus cultures; 18 parts of Saccharomycodes cultures, packed after the oven dry under 40-45 ℃ of temperature after mixing.
The present invention enumerates the several application example to further specify the present invention according to instance:
Application examples 1: adopt composite and highly effective microorganism preparation of the present invention to handle high saliferous aniline, oil of mirbane, nitrophenol wastewater; Treatment process is anaerobic biofilter+BAF; Add composite and highly effective microorganism preparation 30 grams that embodiment 1 makes by every cubic metre of filtrate at every turn; Add every day 1 time, added continuously 15 days; Cultivate domestication after 20 days, treatment effect such as following table:
Oil of mirbane | 1478 | 698.4 | 1.0 | 0.2 | 60-90 | 99.97 |
Nitrophenol | 3000 | 1700 | 2.6 | 0.23 | 60-90 | 99.98 |
Cl ions | 38000 |
Application examples 2: adopt composite and highly effective microorganism preparation of the present invention to handle coal chemical industrial waste water; Treatment process is the filter of A/O+ deposition+aeration and biological; The A/O pond adds composite and highly effective microorganism preparation 20 grams that embodiment 2 makes by every cubic metre of biochemistry pool volume at every turn; Add every day 1 time, added continuously 15 days; BAF adds this composite and highly effective microorganism preparation 40 grams by every cubic meter filler, adds every day 1 time, adds continuously 10 days; Cultivate domestication after 25 days, treatment effect such as following table:
Application examples 3: adopt composite and highly effective microorganism preparation Treatment of Wastewater in Coking of the present invention; Treatment process is acidification hydrolization+BAF+MBR; Add composite and highly effective microorganism preparation 50 grams that embodiment 3 makes by every cubic metre of filler at every turn, add every day once, added continuously 15 days; Cultivate domestication after 30 days, treatment effect such as following table:
Application examples 4: adopt composite and highly effective microorganism preparation of the present invention to handle medicine intermediate waste water; Treatment process is UASB+ catalytic oxidation+BAF; UASB adds composite and highly effective microorganism preparation 30 grams that embodiment 3 makes by every cubic metre of biochemistry pool volume at every turn; Add every day 1 time, added continuously 15 days; Catalytic oxidation and BAF add this composite and highly effective microorganism preparation 30 grams by every cubic metre of filler, add every day 1 time, add continuously 15 days; Cultivate domestication after 30 days, treatment effect such as following table:
The above embodiment is the preferred embodiment that proves absolutely that the present invention lifts, and protection scope of the present invention is not limited thereto.Being equal to that the technician in present technique field is done on basis of the present invention substitutes or conversion, all within protection scope of the present invention.Protection scope of the present invention is as the criterion with claims.
Claims (2)
1. one kind is used for the complex microorganism preparations that used water difficult to degradate is handled, and it is characterized in that said complex microorganism preparations is prepared from following method:
1) selects the bacillus amyloliquefaciens of bacillus to conciliate Pseudomonas aeruginosa and the denitrifying pseudomonas of sugared clostridium spp, Rhodopseudomonas, Bacillus foecalis alkaligenes and denitration Alcaligenes, the black mold of Aspergillus and the yeast saccharomyces cerevisiae and the Candida utilis of aspergillus oryzae and Saccharomycodes of Alkaligenes, above-mentioned bacterial classification, each bacterial classification are carried out seed enlarged culturing step by step respectively;
2) add the clean tap water of 2500mL in the container after disinfects; Add glucose 125g, saltpetre 5g, an ammonium nitrate 4.5g, calcium chloride 1.2g, sal epsom 1.2g, potassium primary phosphate 450mg, inositol 500mg, ferrous sulfate 75mg, manganous sulfate 55mg, zinc sulfate 20mg, boric acid 20mg, potassiumiodide 2mg, Sodium orthomolybdate 0.7mg, copper sulfate 0.07mg respectively; Fully stirring and dissolving, mix; Regulate pH to 7.0; Get the beaker of 5 2000mL after disinfecting again, the above-mentioned solution for preparing is divided equally in 5 beakers each beaker 500mL;
3) in the beaker of above-mentioned 5 nutrient solutions, add wheat bran 450 g, Semen Maydis powder 25 g, soybean cake powder 25 g after sterilising treatment respectively, mixing and stirring;
4) in each beaker, inoculate the kind liquid of bacillus, Rhodopseudomonas, Alkaligenes, Aspergillus, Saccharomycodes enlarged culturing successively; The kind liquid of two strain expanded culture in each Pseudomonas meets 50mL respectively; Put into 35 ℃ thermostat container fermentation culture 36 hours after stirring; Whenever stirred 1 time at a distance from 2 hours between yeast phase; Control pH 6.0 ~ 8.0 in the fermenting process, after fermentation was accomplished, the single kind of mensuration Pseudomonas culture bacteria content reached hundred million of 50-100/gram, fungi content reaches hundred million of 10-50/gram;
5) with cultured fermented product by weight; Press following mixed: 30 parts of Bacillus cultures, 20 parts of Rhodopseudomonas cultures, 15 parts of Alkaligenes cultures, 17 parts of Aspergillus cultures; 18 parts of Saccharomycodes cultures, packed after the oven dry under 40-45 ℃ of temperature after mixing.
2. the application of the described complex microorganism preparations of claim 1 in Treatment of Wastewater in Coking is characterized in that, said complex microorganism preparations is added in the coking chemical waste water biochemistry pool.
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