CN102776138A - Compound microorganism preparation for materialized sludge treatment and method for preparing same - Google Patents
Compound microorganism preparation for materialized sludge treatment and method for preparing same Download PDFInfo
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Abstract
The invention discloses a compound microorganism preparation for materialized sludge treatment and a method for preparing the same. The preparation comprises 2-3.8% of photosynthetic bacteria group, 0.6-1.8% of lactobacillus series, 1-1.8% of saccharomyces cerevisiae, 1-3.8% of gram-positive actinomycetes series, 1-3.8% of filamentous flora of fermentation series, 10-38% of culture medium, and 65-80% of de-ionized water, wherein the content is calculated by weight percent. The method for producing the compound microorganism preparation comprises the following steps of: performing enlarging cultivation on each strain independently by three stages to obtain a production bacterial liquid; and adding de-ionized water and medium culture to a stainless steel fermentation tank, and inoculating each production bacterial liquid for fermentation until the pH value is stabilized in a range of 3.8-4 and the number of live bacteria is not less than 2*10<9>/mL, thereby obtaining the compound microorganism preparation. The preparation is capable of quickly reconstructing the microorganism flora in the materialized sludge and improving the physicochemical states of the sludge, thus prompting decrement, stabilization and harmlessness of the materialized sludge and effectively reducing the water content of the sludge. The method is capable of preparing the compound microorganism preparation.
Description
Technical field
The present invention relates to a kind of complex microorganism preparations and preparation method that materialized mud is handled that be used for.
Background technology
Along with development and national economy and the people pay attention to day by day to environment protection, the sewage amount of focusing on of industrial and agricultural production discharging is increasing, along with and the mud generation that comes is also increasing.
Classify according to State Standard of the People's Republic of China GB/T 23484-2009 " urban wastewater treatment firm sludge disposal "; And the related request of the industry standard CJJ 131-2009 of the People's Republic of China " urban wastewater treatment firm sludge treatment TR ", table 1 is seen in the urban wastewater treatment firm sludge disposal classification that country allows.
The classification of table 1 urban wastewater treatment firm sludge disposal
At present, high density or poisonous and harmful sewage hard to manage such as leather waste water, dyeing waste water, wastewater from chemical industry etc., common processing technological flow is that the materialization processing adds biological process, the characteristics of this sewage treatment process have:
1, the physico-chemical pretreatment to the biochemistry water inlet requires high;
2, additive amount of medicament is directly proportional with the processing water yield, and reagent consumption is bigger, and the materialized mud amount that precipitate and separate is come out is very big.According to statistics, the dyeing and printing sewage treatment station of 10,000 tons of scales, the materialized mud of the water ratio about 80% that produce every day is more than 50 tons;
3, generally all contain multiple hazardous and noxious substances in the mud,, have higher environmental exposure like the compound of heavy metal ion and various forms thereof, the organism of difficult degradation etc.;
4, to be used for the landfill cost minimum for this type of mud; But need take a large amount of land resources; Small sewage treatment plant promptly need take landfill place 10-30 mu every year; Shared landfill place all can not be used effectively filling up between later decades again, and waste sludge very easily to surrounding enviroment particularly underground water cause secondary pollution;
5, burning disposal is to realize sludge reduction, stabilization, innoxious efficient manner, but needs water ratio to be reduced to the active combustion of ability 45% below; Still there is not at present the cost-effective mechanical dehydration mode that can make dehydration rate be reduced to stage like this; Traditional low temperature mummification energy consumption is very big, has aggravated energy shortage, has directly caused handling high;
6, the burning disposal vent gas treatment quite bothers, and exhaust emissions causes environmental pollution, wherein has the Dioxins of a large amount of utmost point difficult degradations, and its transformation period is about 14-273, and toxicity is 900 times of arsenic; As a kind of carcinogens of difficult degradation, Dioxins has thermostability, has only when temperature is higher than 850 ℃; The Dioxins destruction that just is decomposed; So general requirement, the temperature of combustion of incinerator should be higher than this temperature, and guarantees that the Dioxins residence time therein is more than 2 seconds; It is minimum that the concentration of Dioxins is dropped to, and can not work the mischief to the environment and the mankind basically after the discharging; There is not cost-effective mode to make the burning furnace temperature reach so high temperature at present.
In general, in disposal methods such as the transportation of mud, landfill, burning, economical, to reduce water ratio effectively be crucial; Have only water ratio to be reduced to the ideal degree, could fundamentally solve the final of mud and rationally dispose.
Chinese invention patent, application publication number CN 101792247A, " method for sludge treatment that a kind of powerful dehydration combines with the low temperature mummification " discloses the mode that the later mud of a kind of powerful dehydration utilizes the mummification of high-temperature tail gas waste heat again.Its technological deficiency is that usable afterheat need be arranged in appropriate distance, and needs powerful dehydration, does not have general applicability.
Chinese invention patent, publication number CN 1688687A, " new mikrobe and the treatment process of utilizing the organic solid-state thing of this mikrobe " discloses a kind of mikrobe with fluidized solid organic waste.Its technological deficiency is the materialized mud that contains a large amount of inorganic componentss that is not suitable for dyeing waste water and so on, and this type of materialized mud is removed the meaningless act of liquefaction genus again just because of conventional biological treatment is difficult to prove effective with regard to deposition at pretreatment stage.
Chinese invention patent, application publication number CN 101781131 A, " a kind of sludge composting method " discloses through adding auxiliary material, microbe inoculation, the mode of processing organic sludge.Its technological deficiency is characteristics and the use occasion that does not meet materialized mud.
Chinese invention patent, application publication number CN 102173914 A, " a kind of solid-liquid waste oxygen enrichment bioremediation and system " discloses a kind of technology with solid-liquid waste, livestock and poultry resolution, the fermentation of dried spike mixing oxygen enrichment.But still do not meet the characteristics and the follow-up industrial chain use occasion of materialized mud.
Summary of the invention
Technical problem to be solved by this invention provides a kind of complex microorganism preparations and preparation method that materialized mud is handled that be used for; This preparation can rebuild the microorganism species in the materialized mud rapidly; Improve the physics and chemistry state of mud; And then impel materialized mud minimizing, stabilization and innoxious, effectively reduce moisture percentage in sewage sludge; Can obtain this complex microorganism preparations through this preparation method.
For solving the problems of the technologies described above; The complex microorganism preparations that the present invention is used for the materialized mud processing comprises thread flora 1-3.8%, substratum 10-38%, the deionized water 65-80% that photosynthetic bacteria group 2-3.8%, lactobacillus 0.6-1.8%, saccharomyces cerevisiae 1-1.8%, Gram positive actinomycetes crowd 1-3.8%, fermentation are, wherein said per-cent is weight percentage.
Further, above-mentioned photosynthetic bacteria group is one or more in Rhodopseudomonas palustris, rhodopseudomonas acidophilus, colloid rhodopseudomonas, capsula Rhodopseudomonas, the Rhodopseudomonas spheroides.
Further, above-mentioned lactobacillus is one or more in lactobacillus delbruckii, lactobacillus leichmannii, lactobacillus lactis bacteria, lactobicillus bulgaricus, Lactobacterium acidophilum, lactobacterium casei, the plant lactobacillus.
Further, above-mentioned Gram positive actinomycetes crowd is a kind of or whole in cheese streptomycete, the Fu Lankeshi actinomycetes.
Further, the thread flora of above-mentioned fermentation system is in mould one or more of eight zhang island streptoverticilliums, bacterionema matruchotii, distiller's yeast.
Further, above-mentioned substratum is made up of following weight percent raw material: sucrose 25%-75%, potassium primary phosphate 0.5-0.8%, Sodium phosphate, dibasic 0.2-0.6%, Ferric Chloride Hydrated 0.4-1.2%, Zinc vitriol 0.1-0.3%, Salzburg vitriol 0-0.001%, Xiao Suangu 0-0.001%, sal epsom 0.1-0.2%, water 23.7-70%.
The preparation method who is used for the complex microorganism preparations of materialized mud processing comprises the steps:
Step 1, adopt three grades of enlarged culturing to producing bacterium liquid respectively separately each bacterial classification;
Step 2, in the stainless steel fermentor tank of sealing, add deionized water and substratum; Stir also and be heated to 100-140 ℃, be cooled to 25-35 ℃ then rapidly, inoculation step one is cultivated respectively produces bacterium liquid; Maintain the temperature at 25-35 ℃; Ferment, at 3.8-4, the viable bacteria number is not less than 2 * 10 up to the pH value stabilization
9Individual/mL, obtain complex microorganism preparations.
Because being used for the complex microorganism preparations and the preparation method of materialized mud processing, the present invention adopted technique scheme; Be thread flora 1-3.8%, substratum 10-38%, the deionized water 65-80% that this preparation comprises photosynthetic bacteria group 2-3.8%, lactobacillus 0.6-1.8%, saccharomyces cerevisiae 1-1.8%, Gram positive actinomycetes crowd 1-3.8%, fermentation system, wherein content is weight percentage.The preparation method adopts three grades of enlarged culturing to producing bacterium liquid respectively separately each bacterial classification; In the stainless steel fermentor tank, add deionized water and substratum, inoculation is respectively produced bacterium liquid and is fermented, and at 3.8-4, the viable bacteria number is not less than 2 * 10 up to the pH value stabilization
9Individual/mL, obtain complex microorganism preparations.This preparation can rebuild the microorganism species in the materialized mud rapidly, improves the physics and chemistry state of mud, and then impels materialized mud minimizing, stabilization and innoxious, effectively reduces moisture percentage in sewage sludge; Can obtain this complex microorganism preparations through this preparation method.
Embodiment
The complex microorganism preparations that the present invention is used for the materialized mud processing comprises thread flora 1-3.8%, substratum 10-38%, the deionized water 65-80% that photosynthetic bacteria group 2-3.8%, lactobacillus 0.6-1.8%, saccharomyces cerevisiae 1-1.8%, Gram positive actinomycetes crowd 1-3.8%, fermentation are, wherein said per-cent is weight percentage.
Further, above-mentioned photosynthetic bacteria group is one or more in Rhodopseudomonas palustris, rhodopseudomonas acidophilus, colloid rhodopseudomonas, capsula Rhodopseudomonas, the Rhodopseudomonas spheroides.
Further, above-mentioned lactobacillus is one or more in lactobacillus delbruckii, lactobacillus leichmannii, lactobacillus lactis bacteria, lactobicillus bulgaricus, Lactobacterium acidophilum, lactobacterium casei, the plant lactobacillus.
Further, above-mentioned Gram positive actinomycetes crowd is a kind of or whole in cheese streptomycete, the Fu Lankeshi actinomycetes.
Further, the thread flora of above-mentioned fermentation system is in mould one or more of eight zhang island streptoverticilliums, bacterionema matruchotii, distiller's yeast.
Further, above-mentioned substratum is made up of following weight percent raw material: sucrose 25%-75%, potassium primary phosphate 0.5-0.8%, Sodium phosphate, dibasic 0.2-0.6%, Ferric Chloride Hydrated 0.4-1.2%, Zinc vitriol 0.1-0.3%, Salzburg vitriol 0-0.001%, Xiao Suangu 0-0.001%, sal epsom 0.1-0.2%, water 23.7-70%.
The preparation method who is used for the complex microorganism preparations of materialized mud processing comprises the steps:
Step 1, adopt three grades of enlarged culturing to producing bacterium liquid respectively separately each bacterial classification;
Step 2, in the stainless steel fermentor tank of sealing, add deionized water and substratum; Stir also and be heated to 100-140 ℃, be cooled to 25-35 ℃ then rapidly, inoculation step one is cultivated respectively produces bacterium liquid; Maintain the temperature at 25-35 ℃; Ferment, at 3.8-4, the viable bacteria number is not less than 2 * 10 up to the pH value stabilization
9Individual/mL, obtain complex microorganism preparations.
It is the developing direction of environmental protection industry that the present invention utilizes the microbiological treatment materialized mud.Microorganism treatment is a kind of benefit incremental process, and expense reduces with the increase of microbial biomass, is that most economical and effective is administered mud, can not constitute the best solution of safety-problems again.
Complex microorganism preparations of the present invention is not a kind of mikrobe of specific culture, but the multiple microorganisms symbiosis of process screening together, can produce the effective microbe composite fungus agent of multiple function.
In concrete the use; Adopt the gondola water faucet watering can that this microbial preparation evenly is sprayed onto materialized mud material surface; Use suitable turning machinery to turn over then and smash,, change the approach and the product of pollution substance reaction in the materialized mud through in materialized mud, adding this complex microorganism preparations; Nurse one's health the physico-chemical property of mud, reached effective reduction moisture percentage in sewage sludge, while stabilization and innoxious.
Multiple beneficial mikrobe in the five big quasi-microorganisms that this preparation is all existed by the whole ecological system is formed.The preparation method adopts proper proportion and unique zymotechnique; To pass through the good gas property and the anaerobies useful microbe mixed culture that carefully screen; Form diversified microflora, the matrix (food) that benefit materials that these floras produce in growth and secretory substance thereof become separately or grow each other is through a kind of like this symbiosis propagation relation; Formed complicated and stable microecosystem, formation diverse in function, powerful and special advantages.
Photosynthetic bacteria group in this preparation (good gas property and anaerobies) belongs to the autotrophy mikrobe like photosynthetic bacterium and blue algae, and thalline itself contains the protein more than 60%, and is rich in multivitamin, also contains Coenzyme Q10 99.0, antiviral substance and somatomedin; The light and heat that it is accepted with Sewage treatment systems is the energy; Sulfhydryl compound in the sewage and the hydrogen in the hydrocarbon polymer are separated; The change objectionable impurities is innoxious substance; And can organism, obnoxious flavour (hydrogen sulfide etc.) and carbonic acid gas that sewage in the Sewage treatment systems contained, nitrogen etc. be matrix, synthetic carbohydrate, amino acids, vitamins, nitrogen compound, antiviral substance and physiologically active substance etc. are the main strength that promotes other microorganism growth.The metabolic substd of photosynthetic bacteria group can directly be absorbed by each quasi-microorganism, protozoon, metazoan, can also become the nutrient of other microbial reproduction.If photosynthetic bacterium propagation, then other useful microbe also can be bred.
Lactobacillus (anaerobies) is taken as the leading factor with Lactobacterium acidophilum, and it forms lactic acid by the carbohydrate that picked-up photosynthetic bacterium, yeast produce; Lactic acid has very strong sterilizing ability, can effectively suppress the movable and organic rapid corrupt decomposition of harmful microbe, and can decompose not labile under normal conditions xylogen and Mierocrystalline cellulose, and organic matter fermentation is decomposed.
Yeast flora (good gas property) is to utilize photosynthetic bacteria group synthetic amino acid, carbohydrate and other organic substance to produce fermenting power, the fissional sensitization material of synthetic promotion.Yeast provides important subsistence support for promoting other effective microbe (like milk-acid bacteria, actinomycetes) to breed needed matrix (food) in this preparation.In addition, the single cell protein of yeast generation is protozoon, the indispensable nutrient of metazoan growth and breeding indispensable in the Sewage treatment systems of operational excellence.
Gram positive actinomycetes crowd (good gas property) obtains amino acid, nitrogen etc. as matrix from photosynthetic bacterium, produce various antibiotics, VITAMINs and enzyme, can directly suppress pathogenic bacteria.It obtains harmful mould and the needed matrix of bacterial multiplication in advance, thereby suppresses the propagation of harmful mould and bacterium, and creates the living environment of other useful microbe propagation.The mixed clean bacterium effect of actinomycetes and photosynthetic bacterium is more much bigger than the lethality of actinomycetes individual combat.It has Degradation to the material that difficulty is decomposed like xylogen, Mierocrystalline cellulose, chitin etc.
The genus aspergillus that the thread flora (anaerobies) of fermentation system uses during with fermentation alcohol is main body, its can with other mikrobes coexistences, the generation of ester class is had good result.Because the alcohol generative power is strong, can prevents the generation of maggot and other insects, and can eliminate stench.
Therefore each quasi-microorganism is all being brought into play important effect separately, and central role is that photosynthetic bacterium and lactobacillus acidophilus are taken as the leading factor; Its synthesis capability is supporting the activity of other mikrobes, also utilizes the material of other microorganisms simultaneously, forms the relation of coexistence and co-prosperity; Guarantee that this preparation is in stable condition, multiple functional, given play to the powerful energy of multiple flora; Bring up optimum ecology, produce antioxidant, remove oxidizing substance; Eliminate corruption, suppress pathogenic bacteria, form the good environment that is suitable for the active sludge growth; Also produce a large amount of benefit materials of absorbing of being prone to simultaneously, as amino acid, organic acid, Polysaccharides, various VITAMINs, various ENZYMES, somatomedin, microbiotic and antiviral substance etc.Ubiquitous mikrobe in the pending mud environment can be worked in coordination with or dominated to this preparation rapidly; Through symbiosis propagation relation; Formed complicated and stable diversified microecosystem; Through approach such as oxidation, reduction, fermentation, all kinds of organic and inorganic pollutents in the stabilization mud quickly and efficiently.
As a concrete embodiment, each pure strain all can be buied by national DSMZ in this preparation, and the weight percent of each raw material of preparation is: Rhodopseudomonas palustris 3.8%, plant lactobacillus 1.6%, Fu Lankeshi actinomycetes 1.1%; Distiller's yeast is mould 2.3%, substratum 13%, deionized water 77%;
Wherein the weight percent of each raw material of substratum is: sucrose 46%, potassium primary phosphate 0.6%, Sodium phosphate, dibasic 0.4%, Ferric Chloride Hydrated 0.598%, Zinc vitriol 0.2%, Salzburg vitriol 0.001%, Xiao Suangu 0.001%, sal epsom 0.2%, water 52%;
Before compound cultivation, the method that each bacterial classification adopts this specialty to generally acknowledge is respectively separately made three grades of enlarged culturing to producing bacterium liquid, when preparing this preparation; In the stainless steel fermentor tank of sealing, add quantitative deionized water earlier, add substratum, be heated to 120 ℃ under the stirring state; Be cooled to 30 ℃ rapidly, inoculate the production appearance of above-mentioned selected bacterial strain, maintain the temperature at 30 ℃ of fermentations; 3.8, the viable bacteria number is not less than 2 * 10 up to the pH value stabilization
9Individual/mL, can make this preparation and packing warehouse-in.
The present invention is applied to certain dyeing and printing sewage centralized treatment plant, 12000 tons of this sewage work's average treatment amount every days, produces materialized mud 50-60 ton.Need charter the farmland every year and be used for landfill for about 15 mu.After adopting the present invention, newly-built 1000 square meter daylighting booths are handled mud in Sewage Plant, and the later mud of mummification is used for certain proportion to be added clay to and fire building brick and footway brick, and industrial chain is complete, and overall process is respond well.Application result shows, when the present invention is applied to the processing of materialized mud, can adapts to single material and not add the direct stabilization of amendment, innoxious, minimizing, and Sewage treatment systems is had no influence; Mud is through biological dewatered processing, and the ripening degree of becoming thoroughly decomposed reaches more than the 70-90%, and water cut is reduced to below 30%, can satisfy the building materials auxiliary material and add standard, does not influence building material quality, and sintering procedure does not have stink, stacks no mosquitos and flies; Use the newly-built Sewage treatment systems of the present invention because the raising of processing efficiency reaches the initial cost saving 30% of same processing horizontal; The electricity charge are practiced thrift more than 75%, and the integrated operation expense reduces more than 20%, are a kind of sludge treatment new technologies that is fit to energy-saving and emission-reduction at present.
The present invention is through practice test for many years, but has the general flexibility of good mud, reduced investment, unrivaled advantages such as dispersion treatment, no sewage and the mummification of foul smell secondary pollution normal temperature on the spot.
Claims (7)
1. one kind is used for the complex microorganism preparations that materialized mud is handled; It is characterized in that: this microbial preparation comprises thread flora 1-3.8%, substratum 10-38%, the deionized water 65-80% of photosynthetic bacteria group 2-3.8%, lactobacillus 0.6-1.8%, saccharomyces cerevisiae 1-1.8%, Gram positive actinomycetes crowd 1-3.8%, fermentation system, and wherein said per-cent is weight percentage.
2. the complex microorganism preparations that is used for the materialized mud processing according to claim 1, it is characterized in that: said photosynthetic bacteria group is one or more in Rhodopseudomonas palustris, rhodopseudomonas acidophilus, colloid rhodopseudomonas, capsula Rhodopseudomonas, the Rhodopseudomonas spheroides.
3. the complex microorganism preparations that is used for the materialized mud processing according to claim 1, it is characterized in that: said lactobacillus is one or more in lactobacillus delbruckii, lactobacillus leichmannii, lactobacillus lactis bacteria, lactobicillus bulgaricus, Lactobacterium acidophilum, lactobacterium casei, the plant lactobacillus.
4. according to claim 1ly be used for the complex microorganism preparations that materialized mud is handled, it is characterized in that: a kind of or whole in cheese streptomycete, the Fu Lankeshi actinomycetes of said Gram positive actinomycetes crowd.
5. according to claim 1ly be used for the complex microorganism preparations that materialized mud is handled, it is characterized in that: the thread flora of said fermentation system is in mould one or more of eight zhang island streptoverticilliums, bacterionema matruchotii, distiller's yeast.
6. the complex microorganism preparations that is used for the materialized mud processing according to claim 1, it is characterized in that: said substratum is made up of following weight percent raw material: sucrose 25%-75%, potassium primary phosphate 0.5-0.8%, Sodium phosphate, dibasic 0.2-0.6%, Ferric Chloride Hydrated 0.4-1.2%, Zinc vitriol 0.1-0.3%, Salzburg vitriol 0-0.001%, Xiao Suangu 0-0.001%, sal epsom 0.1-0.2%, water 23.7-70%.
7. the described preparation method who is used for the complex microorganism preparations of materialized mud processing of claim 1 is characterized in that present method comprises the steps:
Step 1, adopt three grades of enlarged culturing to producing bacterium liquid respectively separately each bacterial classification;
Step 2, in the stainless steel fermentor tank of sealing, add deionized water and substratum; Stir also and be heated to 100-140 ℃, be cooled to 25-35 ℃ then rapidly, inoculation step one is cultivated respectively produces bacterium liquid; Maintain the temperature at 25-35 ℃; Ferment, at 3.8-4, the viable bacteria number is not less than 2 * 10 up to the pH value stabilization
9Individual/mL, obtain complex microorganism preparations.
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| CN104152378A (en) * | 2014-08-01 | 2014-11-19 | 石家庄开发区德赛化工有限公司 | Compound microbial agent for anaerobic digestion treatment of sludge and production method of compound microbial agent |
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| CN106277659A (en) * | 2015-05-15 | 2017-01-04 | 轻工业环境保护研究所 | A kind of method of sludge organism acidifying dehydration |
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| CN109734263A (en) * | 2017-10-31 | 2019-05-10 | 中环沃野环保有限公司 | A method of domestic sludge is quickly repaired using CPPM collaboration recovery scenario |
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