CN1271171C - Plant edaphon ecological rehabilitation modifier and method for making same - Google Patents
Plant edaphon ecological rehabilitation modifier and method for making same Download PDFInfo
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- CN1271171C CN1271171C CN 200310107802 CN200310107802A CN1271171C CN 1271171 C CN1271171 C CN 1271171C CN 200310107802 CN200310107802 CN 200310107802 CN 200310107802 A CN200310107802 A CN 200310107802A CN 1271171 C CN1271171 C CN 1271171C
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Abstract
The present invention relates to a modifying agent for repairing microbial ecosystem of planting soil, which is characterized in that the present invention is formed by fermenting composite microbial inoculum, soil extract, nutrient culture medium and trace element solution (a solid product also comprises substrate carriers) in solid and liquid anaerobic fermentation modes. The method for preparing the modifying agent for repairing microbial ecosystem of planting soil is characterized in that all used strains are obtained by separating from water body of soil and soil environment, and are optimized by using biological engineering technology of alternative electric fields and plasmogamy technology; optimized strains are mixed with the nutrient culture medium with special components, the soil extract and the trace element solution, and prepared into the modifying agent by technology of anaerobic fermentation of microorganisms. The present invention contains various active microorganisms, enzymes, amino acids, vitamins, organic acids, antioxidation substances and antibiotic substances. The present invention has the advantages that when used, the present invention can form an optimum micro-ecological environment in crop soil to make organic and inorganic nutrients dissoluble, and thus, can promote crop to absorb and convert the nutrients, improve soil aggregate, inhibit growth and propagation of harmful pathogenic bacterium, and decompose remnant pesticide in soil and crop.
Description
Technical field
The present invention relates to a kind of planting soil microbial ecological and repair modifying agent and making method, belong to soil improvement
Technical field.
Background technology
With the degeneration of soil with agingly change an angle and see, be exactly in fact the state that soil has been absorbed in rotten to the core type.The corrupt type soil of narrow sense is meant and has a large amount of spoilage organism in the soil, and generalized is said, even spoilage organism seldom, in case use organic substance, spoilage organism is just bred in a large number and preponderated, and this also is corrupt type soil.
The gas that the organic substance of using in this soil will be converted into reductibility is that alkene, hydrogen sulfide, ammonia etc. discharge, and not only endangers root system of plant, and can be enzyme inhibitor matter by the secondary metabolic conversion, thereby suppress plant growth significantly.In addition, inorganic nutrients is the indissoluble state under reduced state, causes that the hardness of soil increases and micro-shortage.
Especially in the soil of frequent use based on the chemical fertilizer of nitrogenous fertilizer, organic decomposition is rapid, thereby causes producing unbalanced micro-flora in the soil, makes soil become fragile.In this case, the usually easy formation advantage of corrupt type microorganism becomes the basis of improving pathogenic microbes density.
When applying pesticides or weedicide, no matter useful or harmful, the microorganism in the soil all is killed, and causes the decomposition of soil organic matter to be interrupted, and accounts for most spoilage organism at occurring in nature thereafter and also occupy advantage easily in soil.As long as this intermediateness continues, pathogenic bacteria or insect with certain resistance just always can obtain chance, and residual toxicity is strong more, just may promote the resistant gene reorganization of pathogenic bacteria or insect more.
At present, openly be called the patent of plant soil modifier, the patent No. is CN1320675, is made after evenly stirring by polyacrylamide resin, silicate magnesium powder, urea and carrier weighting material; This plant soil modifier moiety and complete processing are simple, and only the physical behavior to soil changes to some extent, can not solve the root problem of soil improvement.Another is the patent of composite biological fertilizer by name openly, the patent No. is CN1380271, composite biological fertilizer,, under aerobic condition, form by sterilized water, glucose, subtilis, bacillus mycoides, azotobacter, streptomyces microflavus, silicate bacteria and yeast through adding strain fermentation successively; Cultivate owing to bacterial classification is not carried out compound domestication, what play a major role in this bacterial manure is metabolic substd, can't the fungus strain benign balance in the soil be exerted an influence, and in fact this product belongs to a kind of fertilizer.
Summary of the invention
The purpose of this invention is to provide the planting soil microbial ecological reparation modifying agent that a kind of tool solid-liquid two kinds of forms, probioticss are counted height and contained the various active metabolic substd.After use, can form optimum ecotope in the crop soil, promote that crop transforms nutrient absorbing, improve Soil structure, suppress harmful growth of pathogenic bacteria and breeding, decompose the remaining agricultural chemicals in soil and the crop.
For realizing above purpose, technical scheme of the present invention provides a kind of solid-state planting soil microbial ecological and repairs modifying agent, it is characterized in that it is prepared by following weight percentages:
Composite bacteria stoste 10-25%
Soil extract 15-30%
Nutritional medium 10-40%
Trace element solution 0.1-2%
Base carrier 20-60%
Described composite bacteria is the mixture of mucormycosis, streptomycete, yeast, photosynthetic bacteria, milk-acid bacteria, vinelandii,
Described soil extract is the liquid that is leached after aseptic soil soaked through 24-36 hour.
Wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 18%
Soil extract 22%
Nutritional medium 20%
Trace element solution 0.5%
Base carrier 39.5%
A kind of liquid planting soil microbial ecological is repaired modifying agent, it is characterized in that it is prepared by following weight percentages:
Composite bacteria stoste 10-25%
Soil extract 36-70%
Nutritional medium 10-40%
Trace element solution 0.1-3%
Described composite bacteria is the mixture of photosynthetic bacteria, genus bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria, milk-acid bacteria,
Described soil extract is the liquid that is leached after aseptic soil soaked through 24-36 hour.
Wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 20%
Soil extract 49.2%
Nutritional medium 30%
Trace element solution 0.8%
Described nutritional medium is one of glucose, starch, Semen Maydis powder, soybean cake powder, yeast powder and sal epsom, potassium primary phosphate, dipotassium hydrogen phosphate, manganous sulfate, ammonium salt or their mixture; Described trace element solution is EDTA-2Na, FeSO
47H
2O, H
3BO
3, CoCl6H
2O, Na
2MoO
4H
2O, Na
2SeO
3, CuCl2H
2One of O or their mixture; Described base carrier is one of blue or green chaff, straw powder, peanut hull meal or their mixture.
All bacterial classifications that adopt all filter out from proportion of crop planting soil and edatope water body; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) soil and edatope water body are sampled separation, each bacterial classification of purifying;
(2) utilize alternating electric field biotechnology and protoplasma integration technology to carry out preferably;
(3) the former bacterial classification that separation is obtained is cultivated in same minimum medium, and the ph value is to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 36 hours, shaking speed 200r/min;
(4) each bacterial classification that grows in the substratum is cultivated under this substratum again, repeat 2-3 time after, obtain composite fungus agent stoste;
(5) with composite fungus agent stoste Ensure Liquid substratum, trace element solution, the solid form product also need add the base carrier, insert in the closed tin behind the uniform mixing, 30-35 ℃ of bottom fermentation 72-96 of solid form product hour, obtain pulverous planting soil microbial ecological after drying is pulverized and repair the modifying agent finished product; Liquid form is under 30-35 ℃, and agitator speed is 60r/min, ferments to get final product in 72 hours.
The present invention adopts the bacterial classification that filters out from multiple soil and edatope water body to mix with nutritional medium, soil extract and the trace element solution of special composition, make by the microbiological anaerobic fermentation technique, it contains various active microorganism, enzyme, amino acid, VITAMIN, organic acid, antioxidant and antibiotics, and the quantity of probiotics viable count has reached 3,000,000,000/gram.
Consider the effect that suppresses disease and organism decomposition aspect, Ideal Match is that the flora of secretion lactic acid combines with saccharomycetic.And in the soil decomposition of fresh organic substance usually based on corrupt type, even adjustment organism of fermentation, the microorganism of the corrupt type of part also can remaining get off, therefore, when considering effectively the utilizing of organic substance, it is not enough only relying on the fermented type microorganism, also must form interaction in conjunction with the synthesis type microorganism.
The present invention repairs modifying agent by what a large amount of screening studies obtained by the planting soil microbial ecological that ferments, the synthesis type microorganism is formed, can in soil, form stable system, the various organic substances that in such system, produce such as amino acid, carbohydrate, VITAMIN, Ester and physiologically active substance etc., not only absorbed by plant easily, and can be become other beneficial microorganism matrix.In addition,, also can produce the material with active effect of many nonionicizations, the effect of highly significant is arranged for the formation of soil water-stable granular structure from the ionic angle.
After using planting soil microbial ecological reparation modifying agent that soil is improved, no matter energy in the soil is organically or inorganic, can not exist with the form of pollution substance, edatope is improved completely, the eliminate the effects of the act injurious factor of root activity, the stripping of soil nutrient will be more smooth and easy also.Simultaneously, the organism major part of using is a soluble conformation by microbial transformation, and therefore, the nitrogen of plant absorbing is many to be organism such as amino acid, in the process of synthetic protein, needs the sugar amount few; Although also there is inorganic nitrogen to be absorbed, because the carbohydrate of solubility is also absorbed simultaneously, less by the consumption of the synthetic carbohydrate of leaf, consequently amount of savings increases, and has realized high-quality and the high yield of farm crop.
Advantage of the present invention is:
1. the special-purpose probiotics that from soil and edatope water body, screens, specially at the improvement of microecological balance in the edatope, the tool specificity;
2. utilize alternating electric field biotechnology and protoplasma integration technology that bacterial classification is optimized, improved the ability to function of bacterial classification greatly;
3. utilize soil extract that bacterial classification is cultivated, make the bacterial classification after the cultivation more can adapt to natural environmental condition;
4. by the microbial transformation effect, nutrient can be dissolved, promote absorption and the growth of crop;
5. by the effect of beneficial microorganism and various antioxidant, physiological metabolism material, suppress the growth of spoilage microorganisms in the soil, also can eliminate the harmful insect worm's ovum simultaneously, improve the resistance against diseases of crop;
6. improve soil aggregate, keep soil from packing together, improve water permeability, decompose the remaining agricultural chemicals in the soil;
7. life-time service, the organic farm products of green, safety is produced in the use that can reduce or stop agricultural chemicals, chemical fertilizer.
Embodiment
Below in conjunction with embodiment the present invention is described in further detail:
The planting soil microbial ecological is repaired the making method of modifying agent:
(1) strain screening from soil and edatope water body takes by weighing by following weight proportion:
Solid form product: mucormycosis (Mucor sp.) 15%, streptomycete (Stretomyces sp.) 20%, yeast (Candida sp.﹠amp; Saccharomyces sp.) 25%, photosynthetic bacteria (Rhodopseudomonassp.) 10%, milk-acid bacteria (Lactobacillus sp.) 20%, vinelandii (Azotobacter sp.) 10%;
Liquid form product: photosynthetic bacteria (Rhodopseudomonas sp.) 20%, genus bacillus (Bcillussp.) 25%, methane-oxidizing bacteria (methyl Zymomonas mobilis Methylomonas methanica) 15%, ammonia oxidation bacteria (Nitrosomonas europaea) 15%, milk-acid bacteria (Lactobacillus sp.) 25%.
Take by weighing substratum by following weight proportion:
Molasses 10%, peptone 3%, glucose 5%, sodium-chlor 0.5%, tween-80 0.1%, dipotassium hydrogen phosphate 0.05%, yeast extract paste 5%, sal epsom 1%, soil extract 30%;
(2) all bacterial classifications are cultivated ph value to 7 in molasses, peptone, glucose, sodium-chlor, tween-80, dipotassium hydrogen phosphate, yeast extract paste, sal epsom and soil extract; Culture temperature is that 33 ℃, time are 36 hours, shaking speed 200r/min; Each bacterial classification that grows in the substratum is cultivated under this substratum again, repeat 3 times after, obtain composite fungus agent stoste;
Take by weighing composite fungus agent and raw material by following weight proportion:
The solid form product:
Composite bacteria stoste 18%
Soil extract 22%
Nutritional medium 20%
Trace element solution 0.5%
Base carrier 39.5%
The liquid form product:
Composite bacteria stoste 20%
Soil extract 49.2%
Nutritional medium 30%
Trace element solution 0.8%
(3) with composite fungus agent Ensure Liquid substratum, trace element solution, the solid form product also need add the base carrier, insert behind the uniform mixing in the closed tin, 33 ℃ of bottom fermentations of solid form product 86 hours obtain pulverous planting soil microbial ecological after drying is pulverized and repair the modifying agent finished product; Liquid form is under 33 ℃, and agitator speed is 60r/min, ferments to get final product in 72 hours.
In use, according to the different soils condition, the solid form product can use separately by different ratios, or mixes the back with fertilizer and use, and the liquid form product can dilute that the back is sprayed or mix the back with certain proportion and water with watering to pour water.
Claims (8)
1. a solid-state planting soil microbial ecological is repaired modifying agent, it is characterized in that it is prepared by following weight percentages:
Composite bacteria stoste 10-25%
Soil extract 15-30%
Nutritional medium 10-40%
Trace element solution 0.1-2%
Base carrier 20-60%
Described composite bacteria is the mixture of mucormycosis, streptomycete, yeast, photosynthetic bacteria, milk-acid bacteria, vinelandii,
Described soil extract is the liquid that is leached after aseptic soil soaked through 24-36 hour.
2. a kind of solid-state planting soil microbial ecological according to claim 1 is repaired modifying agent, it is characterized in that wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 18%
Soil extract 22%
Nutritional medium 20%
Trace element solution 0.5%
Base carrier 39.5%.
3. a liquid planting soil microbial ecological is repaired modifying agent, it is characterized in that it is prepared by following weight percentages:
Composite bacteria stoste 10-25%
Soil extract 36-70%
Nutritional medium 10-40%
Trace element solution 0.1-3%
Described composite bacteria is the mixture of photosynthetic bacteria, genus bacillus, methane-oxidizing bacteria, ammonia oxidation bacteria, milk-acid bacteria,
Described soil extract is the liquid that is leached after aseptic soil soaked through 24-36 hour.
4. a kind of liquid planting soil microbial ecological according to claim 3 is repaired modifying agent, its feature
Be that wherein the optimum weight proportioning of each raw material is:
Composite bacteria stoste 20%
Soil extract 49.2%
Nutritional medium 30%
Trace element solution 0.8%.
5. planting soil microbial ecological according to claim 1 is repaired modifying agent, it is characterized in that described nutritional medium is one of glucose, starch, Semen Maydis powder, soybean cake powder, yeast powder and sal epsom, potassium primary phosphate, dipotassium hydrogen phosphate, manganous sulfate, ammonium salt or their mixture.
6. planting soil microbial ecological according to claim 1 is repaired modifying agent, it is characterized in that described trace element solution is EDTA-2Na, FeSO
47H
2O, H
3BO
3, CoCl6H
2O, Na
2MoO
4H
2O, Na
2SeO
3, CuCl2H
2One of O or their mixture.
7. planting soil microbial ecological according to claim 1 is repaired modifying agent, it is characterized in that described base carrier is one of blue or green chaff, straw powder, peanut hull meal or their mixture.
8. planting soil microbial ecological according to claim 1 is repaired the making method of modifying agent, it is characterized in that all bacterial classifications of employing all filter out from proportion of crop planting soil and edatope water body; Adopt the bacterial classification compound culture method, all bacterial classifications are cultivated in same substratum, it comprises the following steps:
(1) soil and edatope water body are sampled separation, each bacterial classification of purifying;
(2) utilize alternating electric field biotechnology and protoplasma integration technology to carry out preferably;
(3) the former bacterial classification that separation is obtained is cultivated in same minimum medium, and the ph value is to 6.8-7.2; Culture temperature is that 30-35 ℃, time are 36 hours, shaking speed 200r/min;
(4) each bacterial classification that grows in the substratum is cultivated under this substratum again, repeat 2-3 time after, obtain composite fungus agent stoste;
(5) with composite fungus agent stoste Ensure Liquid substratum, trace element solution, the solid form product also need add the base carrier, insert in the closed tin behind the uniform mixing, 30-35 ℃ of bottom fermentation 72-96 of solid form product hour, obtain pulverous planting soil microbial ecological after drying is pulverized and repair the modifying agent finished product; Liquid form is under 30-35 ℃, and agitator speed is 60r/min, ferments to get final product in 72 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107802 CN1271171C (en) | 2003-10-08 | 2003-10-08 | Plant edaphon ecological rehabilitation modifier and method for making same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310107802 CN1271171C (en) | 2003-10-08 | 2003-10-08 | Plant edaphon ecological rehabilitation modifier and method for making same |
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| CN1605614A CN1605614A (en) | 2005-04-13 |
| CN1271171C true CN1271171C (en) | 2006-08-23 |
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| CN 200310107802 Expired - Fee Related CN1271171C (en) | 2003-10-08 | 2003-10-08 | Plant edaphon ecological rehabilitation modifier and method for making same |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101157860B (en) * | 2007-02-14 | 2011-06-01 | 林文辉 | Formula of soil biological oxidation agent and method for making same |
| CN103865826B (en) * | 2012-12-15 | 2016-05-25 | 重庆江合生物科技有限责任公司 | The method of the high gemma rate of a kind of High Density Cultivation bacillus subtilis |
| CN103583106A (en) * | 2013-11-22 | 2014-02-19 | 上海创博生态工程有限公司 | Method for biologically improving saline-alkali soil |
| CN104946550A (en) * | 2014-05-12 | 2015-09-30 | 山东黎昊源生物工程有限公司 | Leaf-protection biological strain repair agent |
| CN105255756A (en) * | 2015-09-29 | 2016-01-20 | 北京市畜牧业环境监测站 | Deodorant complex microbial inoculant and preparation method and application thereof |
| CN105967941A (en) * | 2016-05-04 | 2016-09-28 | 韦小琪 | Preparation method of regenerated fertilizer for restoring soil, and soil restoration method |
| CN106518521A (en) * | 2016-10-28 | 2017-03-22 | 贵州省生物研究所 | Method for rapidly increasing abies fanjingshanensis rhizosphere soil microorganism |
| CN108728103A (en) * | 2018-04-17 | 2018-11-02 | 仁维国际股份有限公司 | The extracting method of geobiont element |
| CN109135754A (en) * | 2018-09-04 | 2019-01-04 | 孙爱友 | A kind of microorganism in situ cultural method for agricultural soil |
| CN113880379A (en) * | 2021-10-29 | 2022-01-04 | 河北地势坤环保工程有限公司 | Composite bottom mud modifier and production process thereof |
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2003
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Owner name: SHANGHAI CHONG BO BIOTECHNOLOGY CO., LTD. Free format text: FORMER OWNER: SHANGHAI CHUANGBO ECOLOGICAL ENGINEERING CO., LTD. Effective date: 20080905 |
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