CN105174491B - For deodorant microorganism formulation of water body and its preparation method and application - Google Patents

For deodorant microorganism formulation of water body and its preparation method and application Download PDF

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CN105174491B
CN105174491B CN201510624972.2A CN201510624972A CN105174491B CN 105174491 B CN105174491 B CN 105174491B CN 201510624972 A CN201510624972 A CN 201510624972A CN 105174491 B CN105174491 B CN 105174491B
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microorganism formulation
water
bacillus pumilus
pseudomonas stutzeri
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CN105174491A (en
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吴山功
管玉柱
陈家庆
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Nanjing Runzhong Environmental Technology Co.,Ltd.
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Nanjing Runzhong Biotechnology Co Ltd
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Abstract

For the deodorant microorganism formulation of water body, the active component of microorganism formulation is consisted of the following composition by weight:25 35 parts of thiobacillus denitrificans, 15 25 parts of bacillus pumilus, 15 25 parts of candida utili, 10 20 parts of lactobacterium acidophilus, 10 20 parts of Pseudomonas stutzeri.Thiobacillus denitrificans are Thiobacillus denitrificans, and preserving number is:ATCC‑25259;Bacillus pumilus is Bacillus pumilus, and preserving number is:ATCC‑27142;Candida utili is Candidautilis, and preserving number is:CCTCC No:AY92020;Lactobacterium acidophilus are Lactobacillus acidophilus, and preserving number is:CGMCC No:8491;Pseudomonas stutzeri is Pseudomonas stutzeri, preserving number:CCTCC No:M209107;Microorganism formulation is reprocessed and microbiological oxidation step after the completion of preparing by sewage disinfection treatment, sewage, you can cleared up black foul sewage water.Beneficial effects of the present invention are:The active height of microorganism formulation, breeding is fast, environment friendly and pollution-free, with low cost, and between each strain compatibility rationally, symbiosis is coordinated, the mutual not advantage of antagonism.It is high for water body odor removal efficient, there is good removal effect particularly with hydrogen sulfide and ammonia.

Description

For deodorant microorganism formulation of water body and its preparation method and application
Technical field
The present invention paddles, the multistep treatment of waste water or sewage, more particularly, to the deodorant microorganism formulation of water body and its Preparation method and application.
Background technology
The water bodys such as municipal lake pool, river course are important parts in human settlement environment in city, but due to its easily pollution, water The features such as environmental carrying capacity is small, water body self-purification ability is poor, it is easy to the receptor as resident living sewage, rainwater and rubbish, from And cause a large amount of consumption of Dissolved Oxygen in Water, and water hypoxia and darkly smelly state are caused, the whole ecosystem crisis is occurred. Urban river occur it is black smelly, it has also become many big or middle cities of China jointly exist pollution problem, have a strong impact on resident living, City image and ecological environment.
When oxygen supply of water body and unbalance oxygen consumption, pollutant produce ammonia nitrogen, hydrogen sulfide, the stink substance such as VFA and The atraments such as iron, manganese sulfide.Those skilled in the art often select biological treatment mode in black and odorous water processing procedure. Using the metabolism of microorganism, the pollutant in decomposition and inversion waste water reaches the purpose for purifying water.Its core is training Domesticating is adapted to the superior microorganism flora of waste water characteristic, and keeps microbial biomass and the work being adapted with Wastewater Pollutant concentration Property, superior microorganism flora is processed in optimal degrading activity, its degradation function is given full play to, system is improved to greatest extent Biological treatment capabilities and treatment effeciency.
But, combined type microorganism formulation, because bacterial activity is different, growing environment is different, causes reproduction inhibition system, altogether Life is uncoordinated, the shortcomings of mutual antagonism.And microorganism formulation also may cause secondary pollution to environment.Existing water body has again High, low for the water body odor removal efficient problem of pollution disposal costs.
The content of the invention
The present invention solves that water body deodorizing process efficiency is low, bacterial activity is poor, degradation capability is weak, to odorous gas hydrogen sulfide The unconspicuous shortcoming of oxidation.
In order to achieve the above object, the present invention provides following technical scheme:It is described for the deodorant microorganism formulation of water body The active component of microorganism formulation is consisted of the following composition by weight:Thiobacillus denitrificans 25-35 parts, bacillus pumilus 15- 25 parts, candida utili 15-25 parts, lactobacterium acidophilus 10-20 parts, Pseudomonas stutzeri 10-20 parts;The thiobacillus denitrificans It is Thiobacillus denitrificans, preserving number is:ATCC-25259;The bacillus pumilus is Bacillus Pumilus, preserving number is:ATCC-27142;The candida utili is Candidautilis, and preserving number is:CCTCC No:AY92020;The lactobacterium acidophilus are Lactobacillus acidophilus, and preserving number is:CGMCC No:8491; The Pseudomonas stutzeri is Pseudomonas stutzeri, preserving number:CCTCC No:M209107.
Wherein, the active component of the microorganism formulation is consisted of the following composition by weight:30 parts of thiobacillus denitrificans;It is short 20 parts of bacillus pumilus, 20 parts of candida utili, 15 parts of lactobacterium acidophilus, 15 parts of Pseudomonas stutzeri.
Wherein, the active component of the microorganism formulation is consisted of the following composition by weight:25 parts of thiobacillus denitrificans;It is short 15 parts of bacillus pumilus, 15 parts of candida utili, 10 parts of lactobacterium acidophilus, 10 parts of Pseudomonas stutzeri.
Wherein, the active component of the microorganism formulation is consisted of the following composition by weight:35 parts of thiobacillus denitrificans;It is short 25 parts of bacillus pumilus, 25 parts of candida utili, 20 parts of lactobacterium acidophilus, 20 parts of Pseudomonas stutzeri.
Wherein, also include, carrier, the carrier is by activated carbon and shitosan in mass ratio 1:1 is made.
For the preparation method of the deodorant microorganism formulation of water body, comprise the following steps:
S1:To thiobacillus denitrificans, bacillus pumilus, candida utili, lactobacterium acidophilus, Pseudomonas stutzeri difference Seed Amplification Culture step by step is carried out, the seed bacterium solution after Amplification Culture is obtained;
S2:The seed bacterium solution that step S1 is obtained is respectively connected to fermentation tank, and inoculum concentration is fermentation medium by weight 5-10%, different Pseudomonas individually carries out fermented and cultured, and the strain of 2 of same Pseudomonas or more than 2 carries out mixing hair Ferment culture;
S3:The seed bacterium solution after fermented and cultured is taken, by weight:Thiobacillus denitrificans 25-35 parts, bacillus pumilus 15- 25 parts, candida utili 15-25 parts, lactobacterium acidophilus 10-20 parts, Pseudomonas stutzeri 10-20 parts, mixed, obtained Mixed bacteria liquid;
S4:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, stands 6-8 hours, is subsequently placed in 4 Low temperature drying under DEG C environment, water content is 6% after drying.
Wherein, the fermentation medium in the step S2 is fluid nutrient medium, is the aqueous solution containing following concentration components: Glucose 40000-60000mg/L, potassium nitrate 1500-2500mg/L, ammonium nitrate 1500-2000mg/L, calcium chloride 400- 500mg/L, magnesium sulfate 450-500mg/L, potassium dihydrogen phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25- 35mg/L, manganese sulfate 20-25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, KI 0.5-1.0mg/L, sodium molybdate 0.2-0.3mg/L and copper sulphate 0.02-0.03mg/L.
Wherein, the fermentation medium PH in the step S2 is 6.0-8.0, and temperature is 30-40 DEG C, fermentation time 24-48 Hour, fermentation process using mechanical agitation and be passed through filtering after air.
For the application of the deodorant microorganism formulation of water body, sewage is entered into microbial reaction pond, regulation pH is 7, by every Cubic meter liquid delivers 10 grams of the microorganism formulation every time, delivers 1 time daily, continuously adds one week, stands 3 days, obtains everywhere Water body after the completion of reason.
Beneficial effects of the present invention are:The active height of microorganism formulation, breeding is fast, environment friendly and pollution-free, with low cost, and Compatibility rationally, coordinate, mutually the not advantage of antagonism by symbiosis between each strain.It is high for water body odor removal efficient, particularly with hydrogen sulfide There is good removal effect with ammonia.
Specific embodiment
Technical scheme will be clearly and completely described below, it is clear that described embodiment is only A part of embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art The every other embodiment obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
Thiobacillus denitrificans are thiobacillus denitrificans (Thiobacillus denitrificans) ATCC-25259;Short and small gemma Bacillus is bacillus pumilus (Bacillus pumilus) ATCC-27142;Candida utili is candida utili (Candida utilis)CCTCC No:AY92020;The lactobacterium acidophilus are lactobacterium acidophilus (Lactobacillus acidophilus)CGMCC No:8491;Pseudomonas stutzeri is Amur vacation Bi born of the same parents bacterium (Pseudomonas stutzeri) CCTCC No:M209107。
Embodiment 1
For the deodorant microorganism formulation of water body, its active component is consisted of the following composition by weight:Thiobacillus denitrificans 30 parts;20 parts of bacillus pumilus, 20 parts of candida utili, 15 parts of lactobacterium acidophilus, 15 parts of Pseudomonas stutzeri.
Microorganism formulation is made up of following steps:
S1:To thiobacillus denitrificans, bacillus pumilus, candida utili, lactobacterium acidophilus, Pseudomonas stutzeri difference Seed Amplification Culture step by step is carried out, the seed bacterium solution after Amplification Culture is obtained.
S2:The seed bacterium solution that step S1 is obtained is respectively connected to fermentation tank, and inoculum concentration is fermentation medium by weight 5-10%, different Pseudomonas individually carries out fermented and cultured, and the strain of 2 of same Pseudomonas or more than 2 carries out mixing hair Ferment culture.
S3:The seed bacterium solution after fermented and cultured is taken, by weight:30 parts of thiobacillus denitrificans, 20 parts of bacillus pumilus produces 20 parts of protein Candida, 15 parts of lactobacterium acidophilus, 15 parts of Pseudomonas stutzeri is mixed, and obtains mixed bacteria liquid.
S4:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, stands 6-8 hours, is subsequently placed in 4 Low temperature drying under DEG C environment, water content is 6% after drying.
Wherein, fermentation medium is fluid nutrient medium, by the aqueous solution containing following concentration components is made:Glucose 40000mg/L, potassium nitrate 1500mg/L, ammonium nitrate 1500mg/L, calcium chloride 400mg/L, magnesium sulfate 450mg/L, biphosphate Potassium 150mg/L, inositol 150mg/L, ferrous sulfate 25mg/L, manganese sulfate 20mg/L, zinc sulfate 5mg/L, boric acid 5mg/L, iodate Potassium 0.5mg/L, sodium molybdate 0.2mg/L and copper sulphate 0.02mg/L.
Wherein, fermentation medium PH is 6.0-8.0, and temperature is 30-40 DEG C, and fermentation time 24-48 hours, fermentation process was adopted With mechanical agitation and be passed through filtering after air.
Its application:
Sewage is entered into microbial reaction pond, regulation pH is 7, and the microorganism formulation is delivered every time by every cubic metre of liquid 10 grams, deliver 1 time daily, continuously add one week, stand 3 days, the water body after the completion of being processed.
After testing, pollutant load compares before and after the sewage for the treatment of, is shown in Table:
Project name Before processing pollutant load (mg/L) Pollutant load (mg/L) after treatment Clearance
NH3-N (ammonia nitrogen) 298 3.2 98.9%
S 42 0.5 98.8%
COD 8524 267 96.9%
Embodiment 2
For the deodorant microorganism formulation of water body, mixed bacteria liquid is made up of following weight portion:25 parts of thiobacillus denitrificans;It is short and small 15 parts of bacillus, 15 parts of candida utili, 10 parts of lactobacterium acidophilus, 10 parts of Pseudomonas stutzeri.Wherein, preparation method and Applicating adn implementing example 1 is identical.
After testing, pollutant load compares before and after the sewage for the treatment of, is shown in Table:
Project name Before processing pollutant load (mg/L) Pollutant load (mg/L) after treatment Clearance
NH3-N (ammonia nitrogen) 298 13.2 95.6%
S 42 0.4 99.0%
COD 8524 267 96.9%
Embodiment 3
For the deodorant microorganism formulation of water body, mixed bacteria liquid is made up of following weight portion:35 parts of thiobacillus denitrificans;It is short and small 25 parts of bacillus, 25 parts of candida utili, 20 parts of lactobacterium acidophilus, 20 parts of Pseudomonas stutzeri.Wherein, preparation method and Applicating adn implementing example 1 is identical.
After testing, pollutant load compares before and after the sewage for the treatment of, is shown in Table:
Project name Before processing pollutant load (mg/L) Pollutant load (mg/L) after treatment Clearance
NH3-N (ammonia nitrogen) 298 2.7 99.1%
S 42 0.7 98.3%
COD 8524 267 96.9%
Understand that the comprehensive treatment effect of embodiment 1 is better than, embodiment 2 and 3 by detection.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, should all contain Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.

Claims (10)

1. be used for the deodorant microorganism formulation of water body, it is characterised in that the active component of the microorganism formulation by weight by Following component is constituted:Thiobacillus denitrificans 25-35 parts, bacillus pumilus 15-25 parts, candida utili 15-25 parts, acidophilus breast Sour bacterium 10-20 parts, Pseudomonas stutzeri 10-20 parts;The thiobacillus denitrificans are Thiobacillus denitrificans, are protected Tibetan number is:ATCC-25259;The bacillus pumilus is Bacillus pumilus, and preserving number is:ATCC-27142;It is described Candida utili is Candida utilis, and preserving number is:CCTCC No:AY92020;The lactobacterium acidophilus are Lactobacillus acidophilus, preserving number is:CGMCC No:8491;The Pseudomonas stutzeri is Pseudomonas stutzeri, preserving number:CCTCC No:M209107.
2. microorganism formulation according to claim 1, it is characterised in that the active component of the microorganism formulation is by weight Part consists of the following composition:30 parts of thiobacillus denitrificans;20 parts of bacillus pumilus, 20 parts of candida utili, lactobacterium acidophilus 15 parts, 15 parts of Pseudomonas stutzeri.
3. microorganism formulation according to claim 1, its characteristic is that the active component of the microorganism formulation is by weight Part consists of the following composition:25 parts of thiobacillus denitrificans;15 parts of bacillus pumilus, 15 parts of candida utili, lactobacterium acidophilus 10 parts, 10 parts of Pseudomonas stutzeri.
4. microorganism formulation according to claim 1, its characteristic is that the active component of the microorganism formulation is by weight Part consists of the following composition:35 parts of thiobacillus denitrificans;25 parts of bacillus pumilus, 25 parts of candida utili, lactobacterium acidophilus 20 parts, 20 parts of Pseudomonas stutzeri.
5. the microorganism formulation according to claim any one of 2-4, its characteristic is also to include, carrier, the carrier by Activated carbon and shitosan in mass ratio 1:1 is made.
6. the preparation method of the microorganism formulation deodorant for water body as described in claim any one of 1-5, it is characterised in that Comprise the following steps:
S1:Thiobacillus denitrificans, bacillus pumilus, candida utili, lactobacterium acidophilus, Pseudomonas stutzeri are carried out respectively Seed Amplification Culture, obtains the seed bacterium solution after Amplification Culture step by step;
S2:The seed bacterium solution that step S1 is obtained is respectively connected to fermentation tank, and inoculum concentration is the 5- of fermentation medium by weight 10%, different Pseudomonas individually carries out fermented and cultured, and the strain of 2 of same Pseudomonas or more than 2 carries out mixed fermentation Culture;
S3:The seed bacterium solution after fermented and cultured is taken, by weight:Thiobacillus denitrificans 25-35 parts, bacillus pumilus 15-25 parts, Candida utili 15-25 parts, lactobacterium acidophilus 10-20 parts, Pseudomonas stutzeri 10-20 parts, mixed, obtained Mixed Microbes Liquid;
S4:By mixed bacteria liquid and carrier according to 1:1 weight stirs than mixing, stands 6-8 hours, is subsequently placed in 4 DEG C of rings Low temperature drying under border, water content is 6% after drying.
7. the preparation method of the microorganism formulation deodorant for water body according to claim 6, it is characterised in that the step Fermentation medium in rapid S2 is fluid nutrient medium, is the aqueous solution containing following concentration components:Glucose 40000- 60000mg/L, potassium nitrate 1500-2500mg/L, ammonium nitrate 1500-2000mg/L, calcium chloride 400-500mg/L, magnesium sulfate 450-500mg/L, potassium dihydrogen phosphate 150-200mg/L, inositol 150-250mg/L, ferrous sulfate 25-35mg/L, manganese sulfate 20- 25mg/L, zinc sulfate 5-10mg/L, boric acid 5-10mg/L, KI 0.5-1.0mg/L, sodium molybdate 0.2-0.3mg/L and sulfuric acid Copper 0.02-0.03mg/L.
8. the preparation method of the microorganism formulation deodorant for water body according to claim 7, it is characterised in that the step Fermentation medium PH in rapid S2 is 6.0-8.0, and temperature is 30-40 DEG C, and fermentation time 24-48 hours, fermentation process used machine The air that tool is stirred and is passed through after filtering.
9. microorganism formulation as claimed in claim 1 is used for the deodorant application of water body.
10. microorganism formulation according to claim 9 is used for the deodorant application of water body, and its characteristic is to enter sewage Microbial reaction pond, regulation pH is 7, and 10 grams of the microorganism formulation is delivered every time by every cubic metre of liquid, is delivered 1 time daily, Continuously add one week, stand 3 days, the water body after the completion of being processed.
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CN107601678B (en) * 2017-09-26 2018-09-14 惠州学院 A kind of cities and towns black and odorous water and sediment in-situ quickly administer material
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