CN105400723A - Composite microbial preparation used for river treatment - Google Patents
Composite microbial preparation used for river treatment Download PDFInfo
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- CN105400723A CN105400723A CN201510949257.6A CN201510949257A CN105400723A CN 105400723 A CN105400723 A CN 105400723A CN 201510949257 A CN201510949257 A CN 201510949257A CN 105400723 A CN105400723 A CN 105400723A
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/101—Sulfur compounds
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/105—Phosphorus compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
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- Biodiversity & Conservation Biology (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
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Abstract
The invention relates to the technical field of microbes, and especially discloses a composite microbial preparation used for river treatment. The composite microbial preparation used for river treatment comprises a compound microorganism, and the compound microorganism is composed of the following raw materials in percent by weight: 1-2% of nitrosococcus, 5-8% of thiobacillus thiooxidans, 8-15% of paracoccus denitrificans, 20-30% of bacillus spp, 8-18% of rhodospira, 6-15% of lactobacillus spp, 10-15% of saccharomyces cerevisiae, and 20-30% of bacillus subtilis. After being poured into polluted water, the composite microbial preparation used for river treatment is capable of obviously improving the water body blackening smelliness problem, reducing water-quality ammonium nitrogen and total phosphorus content, decomposing hydrogen sulfide and other harmful substances and improving water body environment. The composite microbial preparation used for river treatment is capable of improving the absorption utilization efficiency of water plants on nutrition elements and obviously improving the water body transparency, and possesses the advantages of stabile microbial preparation, strong purification capability, simple preparation, low cost and applicability to river treatment.
Description
Technical field
The present invention relates to microbial technology field, particularly relate to a kind of complex microorganism preparations for river regulation.
Background technology
Along with population growth and urban construction and development, a large amount of mankind's activities creates tremendous influence to natural water, and a large amount of pollutent directly enters in river course, causes the organism in water body, ammonia nitrogen, total phosphorus, H
2the objectionable impuritiess such as S continue to increase, the natural purifying ability wretched insufficiency of water body.
The impact that microbiobacterial agent effectively can promote the detergent power opposing water pollutions of natural water is added on eutrophication or polluted-water.Microbial preparation is applied to river course and improves water ecological setting, suppress to kill pathogenic micro-organism, probiotics is had the advantage level.But because natural water is administered at the early-stage, the microbiobacterial agent for river eutrophication is less.And be applicable to the microbiobacterial agent of trade effluent and be not suitable for the river water body differed greatly with trade effluent water quality.
Summary of the invention
For the defect that prior art exists, the invention provides a kind of microbial inoculum and stablize, detergent power is strong, the complex microorganism preparations being applicable to river regulation that preparation is simple, cost is low.
Realize foregoing invention object, the present invention adopts following technical scheme:
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: nitrous acid pure coccus 1 ∽ 2%, thiobacillus thiooxidant 5 ∽ 8%, Paracoccus denitrificans 8 ∽ 15%, genus bacillus 20 ∽ 30%, red spirobacteria 8 ∽ 18%, lactobacillus 6 ∽ 15% and S. cervisiae 10 ∽ 15%, Bacillus subtilus 20 ∽ 30%.
As preferably, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 5%, Paracoccus denitrificans 8%, genus bacillus 30%, red spirobacteria 10%, lactobacillus 15% and S. cervisiae 10%, Bacillus subtilus 20%.
As preferably, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 7%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 8%, lactobacillus 8% and S. cervisiae 10%, Bacillus subtilus 30%.
As preferably, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 1%, thiobacillus thiooxidant 8%, Paracoccus denitrificans 15%, genus bacillus 20%, red spirobacteria 15%, lactobacillus 6% and S. cervisiae 10%, Bacillus subtilus 25%.
As preferably, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 6%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 18%, lactobacillus 9% and S. cervisiae 10%, Bacillus subtilus 20%.
As preferably, exist with the form of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material mixture.
As preferably, also comprise solid adjuvant material, described auxiliary material and the weight ratio of complex microorganism are 4:1.
A kind of complex microorganism preparations for river regulation of the present invention, pure for nitrous acid coccus, thiobacillus thiooxidant, Paracoccus denitrificans, genus bacillus, red spirobacteria, lactobacillus and the profitable strain such as S. cervisiae, Bacillus subtilus are carried out scientific and reasonable integration, the complex micro organism fungicide cultivated, have in stable condition, strong adaptability, collaborative symbiosis, the features such as miscellaneous bacteria rate is extremely low.Drop in polluted-water, utilize bacteria metabolism movable, reduce water pollutant concentration, obviously can improve water body and to turn black smelly problem, reduce Water quality ammonia nitrogen and total phosphorous, the objectionable impuritiess such as decomposing hydrogen sulfide, improve water body environment; Can improve the absorption and use efficiency of waterplant to nutritive element, significantly improve water transparency, have microbial inoculum and stablize, detergent power is strong, the advantage prepare simply, cost is low, being suitable for river regulation.
Embodiment
Below by embodiment, the present invention is described further.
Embodiment 1
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 5%, Paracoccus denitrificans 8%, genus bacillus 30%, red spirobacteria 10%, lactobacillus 15% and S. cervisiae 10%, Bacillus subtilus 20%.
A kind of preparation process of the complex microorganism preparations for river regulation is:
1) the independent enlarged culturing of each microorganism:
The pure coccus of nitrous acid: the pure coccus of nitrous acid is on Nitrite bacteria substratum, and 28-30 DEG C, first slant culture, then secondary seed solution is cultivated, and mixing fermentation culture reaches 1-2 × 10 to bacterium is dense
8individual/mL.Described nitrococcus substratum: ammonium sulfate 0.5g, sodium-chlor 0.3g, ferrous sulfate 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 7.5g, distilled water 1000mL, PH=8.
Thiobacillus thiooxidant: on thiobacillus thiooxidant is cultivated in thiosulphate solid medium, first slant culture, then secondary seed solution is cultivated, and mixing fermentation culture reaches 1-2 × 10 to bacterium is dense
8individual/.Thiosulphate solid medium: peptone 10g, yeast powder 5g, bovine bile powder 8g, Sodium Citrate 10g, Sulfothiorine 10g, ironic citrate 1g, bromothymol blue 0.04g, thymol blue 0.04g;
Genus bacillus: genus bacillus is on beef-protein medium, and 28-30 DEG C, makes primary inclined plane and cultivate, is then inoculated into triangular flask and does the cultivation of vibration secondary liquid, reach 3-4 × 10 to bacterium number
8individual.
Yeast saccharomyces cerevisiae: yeast is on lactic acid-Potato-dextrose substratum, and 28-30 DEG C, makes primary inclined plane and cultivate, is then inoculated into and does vibration secondary liquid in triangular flask and be cultured to bacterium number and reach 1.0-2.5 × 10
8individual/mL.
Red spirobacteria: photosynthetic bacterium stoste is inoculated in liquid test tube with cover, 28-30 DEG C, illumination cultivation 3-5 days, obtains red spirobacteria bacteria suspension, the bacteria suspension getting 1% (volume ratio) puts into the substratum of 500mL triangular flask deactivation, with Fan Shi substratum multiplication culture, constant temperature oscillation shaking table 28-30 DEG C, cultivate 36-72 hour, obtain the secondary pure growth of photosynthetic bacterium, the photosynthetic bacterium secondary seed getting 1% (volume ratio) is inoculated in by sodium acetate 2g, 1% (mass ratio) ammonium chloride 1g, potassium primary phosphate 0.1g, magnesium sulfate 0.5g, sodium-chlor 0.2g, amine molybdate 3g, yeast extract 1g, calcium chloride 0.05g, fermentation propagation in the substratum that iron(ic) chloride 0.05g forms, 28-30 DEG C of illumination static gas wave refrigerator or not illumination shaking culture, obtain bacterium number and reach 1.0-2.0 × 10
8individual/mL fermenting agent,
Denitrogenation coccus: Sodium Nitrite 1g, magnesium sulfate 0.03g, manganous sulfate 0.01g, dipotassium hydrogen phosphate 0.75g, sodium carbonate 1g, SODIUM PHOSPHATE, MONOBASIC 0.25g, distilled water 1000mL, PH=7.2; Make primary inclined plane to cultivate, be then inoculated into and do vibration secondary liquid in triangular flask and be cultured to bacterium number and reach 3-4 × 10
8individual.
Bacillus subtilus: Bacillus subtilus is on beef-protein medium, and 28-30 DEG C, makes primary inclined plane and cultivate, is then inoculated into triangular flask and does the cultivation of vibration secondary liquid, reach 3-4 × 10 to bacterium number
8individual/mL.
2) lyophilized powder is made
Each for described complex microorganism raw material is prepared fermented liquid respectively, after mass ratio mixing, makes lyophilized powder according to this area ordinary method.
River course, Zhejiang Hangzhou Jianggan District, is subject to the impact of neighbouring sanitary sewage discharge throughout the year.COD is about 40mg/L, and ammonia nitrogen concentration is about 15mg/L, and total phosphorus is about 1.5mg/L, and it is smelly that too high nitrogen and phosphorus content causes water body to turn black, and transparency only has 15cm.Belong to the black smelly river course of moderate.Start a kind of complex microorganism preparations for river regulation used described in the present embodiment in May, 2015, after river dilution, evenly splash in water body, after 15 days, transparency brings up to 40cm; Dissolved oxygen rises to 4mg/L from 1.5mg/L, and ammonia nitrogen drops to 6mg/L, and total phosphorus drops to 1mg/L; After 45 days, transparency brings up to 80cm; Dissolved oxygen rises to 6.2mg/L from 4mg/L, and ammonia nitrogen drops to 2mg/L, and total phosphorus drops to 0.4mg/L, reaches " water environment quality standard " GB3838-2002 " in five class water levels, water body improve clearly.
Embodiment 2
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 7%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 8%, lactobacillus 8% and S. cervisiae 10%, Bacillus subtilus 30%.
Cultivation and the complex microorganism preparations making method of each microorganism are identical with embodiment 1.
Embodiment 3
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 1%, thiobacillus thiooxidant 8%, Paracoccus denitrificans 15%, genus bacillus 20%, red spirobacteria 15%, lactobacillus 6% and S. cervisiae 10%, Bacillus subtilus 25%.
Cultivation and the complex microorganism preparations making method of each microorganism are identical with embodiment 1.
Embodiment 4
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 6%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 18%, lactobacillus 9% and S. cervisiae 10%, Bacillus subtilus 20%.
The cultivation of each microorganism is as identical in embodiment 1, is uniformly mixed by mentioned microorganism microbial inoculum afterwards by above-mentioned mass ratio, and low temperature (≤60 DEG C) is dry, and moisture content controls, below 20%, to obtain complex microorganism preparations.
Embodiment 5
A kind of complex microorganism preparations for river regulation, comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 1%, thiobacillus thiooxidant 5%, Paracoccus denitrificans 12%, genus bacillus 20%, red spirobacteria 10%, lactobacillus 12% and S. cervisiae 15%, Bacillus subtilus 25%.
The cultivation of each microorganism is as identical in embodiment 1, is undertaken being uniformly mixed formation complex microorganism afterwards, using zeolite powder (40-80 order) as carrier by cultivating kind of agent above by above-mentioned mass ratio; The ratio of zeolite powder and complex microorganism is that 1:4 obtains microbial inoculum and solid adjuvant material mixture.
River course, Zhejiang Hangzhou Shangcheng District, receive throughout the year upstream sewage import and neighbouring garbage loading embeading factory impact.COD is about 75mg/L, and ammonia nitrogen concentration is about 20mg/L, and total phosphorus is about 2.5mg/L, and river turns black smelly throughout the year, and transparency only has 7cm.Belong to highly black smelly river course.Start a kind of complex microorganism preparations for river regulation used described in the present embodiment in July, 2015, after river dilution, evenly splash in water body, after 15 days, transparency brings up to 20cm; Dissolved oxygen rises to 2mg/L from 1mg/L, and ammonia nitrogen drops to 9mg/L, and total phosphorus drops to 1.5mg/L; After 60 days, transparency brings up to 25cm; Dissolved oxygen rises to 4mg/L from 2mg/L, and ammonia nitrogen drops to 3mg/L, and total phosphorus drops to 0.8mg/L, and the clearance of ammonia nitrogen and total phosphorus reaches 75%.
Be only preferred embodiment of the present invention in sum, be not used for limiting practical range of the present invention, all equivalences done according to the content of the application's the scope of the claims change and modify, and all should be technology category of the present invention.
Claims (7)
1. the complex microorganism preparations for river regulation, it is characterized in that: comprise complex microorganism, described complex microorganism is made up of the raw material of following weight percent: nitrous acid pure coccus 1 ∽ 2%, thiobacillus thiooxidant 5 ∽ 8%, Paracoccus denitrificans 8 ∽ 15%, genus bacillus 20 ∽ 30%, red spirobacteria 8 ∽ 18%, lactobacillus 6 ∽ 15% and S. cervisiae 10 ∽ 15%, Bacillus subtilus 20 ∽ 30%.
2. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 5%, Paracoccus denitrificans 8%, genus bacillus 30%, red spirobacteria 10%, lactobacillus 15% and S. cervisiae 10%, Bacillus subtilus 20%.
3. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 7%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 8%, lactobacillus 8% and S. cervisiae 10%, Bacillus subtilus 30%.
4. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 1%, thiobacillus thiooxidant 8%, Paracoccus denitrificans 15%, genus bacillus 20%, red spirobacteria 15%, lactobacillus 6% and S. cervisiae 10%, Bacillus subtilus 25%.
5. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: described complex microorganism is made up of the raw material of following weight percent: the pure coccus of nitrous acid 2%, thiobacillus thiooxidant 6%, Paracoccus denitrificans 10%, genus bacillus 25%, red spirobacteria 18%, lactobacillus 9% and S. cervisiae 10%, Bacillus subtilus 20%.
6. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: exist with the form of lyophilized powder, microbial inoculum or microbial inoculum and solid adjuvant material mixture.
7. a kind of complex microorganism preparations for river regulation according to claim 1, is characterized in that: also comprise solid adjuvant material, and described auxiliary material and the weight ratio of complex microorganism are 1:4.
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011020A (en) * | 2016-07-01 | 2016-10-12 | 宁波市川宁环保科技有限公司 | Compound microbial preparation for processing polluted water and preparing method thereof |
| CN106282061A (en) * | 2016-08-16 | 2017-01-04 | 南京中科水治理股份有限公司 | For complex microorganism preparations that black-odor riverway is administered and its preparation method and application |
| CN106635883A (en) * | 2016-11-09 | 2017-05-10 | 东营晟宇环保科技有限公司 | Composite microbial preparation for treating watercourse organic matter |
| CN108358322A (en) * | 2018-02-07 | 2018-08-03 | 山东九生物科技股份有限公司 | A kind of strain formula and preparation method thereof for administering black and odorous water |
| CN110117567A (en) * | 2019-06-04 | 2019-08-13 | 西安工程大学 | A kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization |
| CN110205269A (en) * | 2019-06-04 | 2019-09-06 | 广东子辉生态环境科技有限公司 | A kind of Tiny ecosystem flora and its application method and application for repairing artificial swamp |
| CN111534463A (en) * | 2020-05-06 | 2020-08-14 | 河南林泉环保科技有限公司 | Composite microbial preparation for river treatment |
| CN112062292A (en) * | 2020-09-01 | 2020-12-11 | 湖南景翌湘台环保高新技术开发有限公司 | Compound bacterium agent for purifying black and odorous water body |
| CN112077126A (en) * | 2020-09-17 | 2020-12-15 | 杭州楠大环保科技有限公司 | Household kitchen waste phase-change water production degradation treatment system and treatment method thereof |
| CN112760261A (en) * | 2021-01-28 | 2021-05-07 | 海宁一泓环境科技有限公司 | Composite microbial preparation for river treatment |
-
2015
- 2015-12-18 CN CN201510949257.6A patent/CN105400723A/en active Pending
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106011020A (en) * | 2016-07-01 | 2016-10-12 | 宁波市川宁环保科技有限公司 | Compound microbial preparation for processing polluted water and preparing method thereof |
| CN106282061A (en) * | 2016-08-16 | 2017-01-04 | 南京中科水治理股份有限公司 | For complex microorganism preparations that black-odor riverway is administered and its preparation method and application |
| CN106635883A (en) * | 2016-11-09 | 2017-05-10 | 东营晟宇环保科技有限公司 | Composite microbial preparation for treating watercourse organic matter |
| CN108358322A (en) * | 2018-02-07 | 2018-08-03 | 山东九生物科技股份有限公司 | A kind of strain formula and preparation method thereof for administering black and odorous water |
| CN110117567B (en) * | 2019-06-04 | 2023-03-03 | 西安工程大学 | Paracoccus denitrificans strain screening and application thereof in deodorization |
| CN110117567A (en) * | 2019-06-04 | 2019-08-13 | 西安工程大学 | A kind of Paracoccus denitrificans bacterial strain screening and its application in deodorization |
| CN110205269A (en) * | 2019-06-04 | 2019-09-06 | 广东子辉生态环境科技有限公司 | A kind of Tiny ecosystem flora and its application method and application for repairing artificial swamp |
| CN111534463A (en) * | 2020-05-06 | 2020-08-14 | 河南林泉环保科技有限公司 | Composite microbial preparation for river treatment |
| CN112062292A (en) * | 2020-09-01 | 2020-12-11 | 湖南景翌湘台环保高新技术开发有限公司 | Compound bacterium agent for purifying black and odorous water body |
| CN112062292B (en) * | 2020-09-01 | 2023-12-01 | 湖南景翌湘台环保高新技术开发有限公司 | Black and odorous water body purifying composite microbial agent |
| CN112077126A (en) * | 2020-09-17 | 2020-12-15 | 杭州楠大环保科技有限公司 | Household kitchen waste phase-change water production degradation treatment system and treatment method thereof |
| CN112077126B (en) * | 2020-09-17 | 2023-07-25 | 杭州楠大环保科技有限公司 | Household kitchen waste phase-change water-making degradation treatment system and treatment method thereof |
| CN112760261A (en) * | 2021-01-28 | 2021-05-07 | 海宁一泓环境科技有限公司 | Composite microbial preparation for river treatment |
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