CN106635883A - Composite microbial preparation for treating watercourse organic matter - Google Patents
Composite microbial preparation for treating watercourse organic matter Download PDFInfo
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- CN106635883A CN106635883A CN201610982439.8A CN201610982439A CN106635883A CN 106635883 A CN106635883 A CN 106635883A CN 201610982439 A CN201610982439 A CN 201610982439A CN 106635883 A CN106635883 A CN 106635883A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/347—Use of yeasts or fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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Abstract
The invention discloses a composite microbial preparation for treating watercourse organic matter. The composite microbial preparation is prepared from the following ingredients in percentage by weight: 10 to 20% of pseudomonas, 10 to 15% of vibrio, 10 to 15% of flavobacterium, 10 to 15% of pure nitrite coccus, 10 to 15% of thiobacillus thiooxidant, 10 to 20% of bacillus subtilis, 5 to 15% of bacillus licheniformis, 5 to 10% of aneurinibacillus aneurinilyticus, 5 to 10% of saccharomycetes, 5 to 10% of micrococcus denitrificans and 5 to 10% of actinomycetes. Compared with the prior art, the composite microbial preparation disclosed by the invention can rapidly decompose organic wastes, and effectively inhibits growth of harmful microorganisms; when the composite microbial preparation is put into polluted water, by utilizing metabolic activity of the microorganisms, concentration of pollutants in the water is reduced, the problems of blackening and smelliness of the water can be obviously improved, ammonia nitrogen content and total phosphorus content in the water are reduced, harmful substances such as hydrogen sulfide are decomposed, the water environment is improved, and transparency of the water is obviously improved; the composite microbial preparation has the advantages of simplicity for preparation, low cost and application to watercourse treatment, and has great economic and environmental-friendly significance for watercourse treatment.
Description
Technical field
The present invention relates to microbial technology field, more particularly to a kind of complex microorganism system administered for river course organic matter
Agent.
Background technology
As population increases and urban construction and development, substantial amounts of mankind's activity generates tremendous influence to natural water, greatly
Amount pollutant is directly discharged into river course, causes the harmful substances such as the organic matter in water body, ammonia nitrogen to continue to increase, water body naturally only
Change ability wretched insufficiency.Microbial bacterial agent is added to eutrophication or polluted-water can effectively lift the purification energy of natural water
Power resists the impact of water pollution.
It is domestic at present general for the improvement of lake pollution using desilting or the method for implantation methods water plant, both
Method is required for larger manpower and materials, pollutes control expense height, and dredging method can also cause secondary pollution；And plant species
Plant method needs to drain lake water, the method and unrealistic when relatively large lake is administered.Complex microorganism preparations be by two kinds or
Two or more and mutual not microorganism formulation made by the beneficial microbe bacterial classification of antagonism.Beneficial bacterium using the physiological metabolism of itself come
The undesirable elements such as the organic matter in water, ammonia nitrogen, nitrite, hydrogen sulfide in decomposition water are processed, is purified water, solve various poison
The quality problem that element is caused, reduces the risk of cultivated animals, and available energy suppresses the generation of cultivated animals symptom, reduces cultivated animals
Demand to antibiotic, using the interaction of composite biological agent beneficial bacterium healthy aquaculture is reached.
It is an object of the invention to microorganism formulation is applied into river course improves water ecological setting, complex microorganism is studied
Preparation energy efficient degradation debirs, solve the weak existing method degradation capability for processing sewage, time length, the skill of secondary pollution
Art problem.
The content of the invention
The complex microorganism preparations of this efficient degradation debirs of the present invention, containing pseudomonad, vibrios, yellow bar
The pure coccus of bacterium, nitrous acid, thiobacillus thiooxidant, bacillus subtilis, bacillus licheniformis, bacillus aneurinolytieus, saccharomycete,
Denitrogenation coccus, actinomyces, in described complex microorganism preparations, the bacterium number of pseudomonad account for the percentage of total bacteria count be 10 ~
20%, it is 10 ~ 15% that the bacterium number of vibrios accounts for the percentage of total bacteria count, and it is 10 ~ 15% that the bacterium number of Flavobacterium accounts for the percentage of total bacteria count,
It is 10 ~ 15% that the bacterium number of the pure coccus of nitrous acid accounts for the percentage of total bacteria count, and the bacterium number of thiobacillus thiooxidant accounts for the percentage of total bacteria count and is
10 ~ 15%, it is 10 ~ 20% that the bacterium number of bacillus subtilis accounts for the percentage of total bacteria count, and the bacterium number of bacillus licheniformis accounts for total bacteria count
Percentage be 5 ~ 15%, it is 5 ~ 10% that the bacterium number of bacillus aneurinolytieus accounts for the percentage of total bacteria count, and saccharomycetic bacterium number is accounted for
The percentage of total bacteria count is 5 ~ 10%, and it is 5 ~ 10% that the bacterium number of denitrogenation coccus accounts for the percentage of total bacteria count, and actinomycetic bacterium number is accounted for always
The percentage of bacterium number is 5 ~ 10%.
Preferably, described complex microorganism is made up of the raw material of following percentage by weight：Pseudomonad 15%, vibrios
10%th, the pure coccus 15% of Flavobacterium 10%, nitrous acid, thiobacillus thiooxidant 13%, bacillus subtilis 10%, bacillus licheniformis 5%, solution
Thiamine bacillus 5%, saccharomycete 7%, denitrogenation coccus 5%, actinomyces 5%.
Preferably, in described complex microorganism preparations, described total bacteria count is at 1,000,000,000 more than per gram.
Preferably, in the form of freeze-dried powder, microbial inoculum or microbial inoculum and solid adjuvant material mixture.
Preferably, described solid adjuvant material is zeolite powder（40-80 mesh）, the ratio of zeolite powder and complex microorganism is 1：
4 obtain microbial inoculum and solid adjuvant material mixture.
Preferably, the above-mentioned bacterial classification employed in the present invention for this area conventional bacterial classification, can commercially,
Or from China General Microbiological culture presevation administrative center（CGMCC）Purchase.
The preparation method of the complex microorganism preparations of this efficient degradation debirs of the present invention can be according to routine
Method, first carries out the amplification culture of single bacterial classification, then counts, and is mixed according still further to ratio.
There is excellent synergy between the microorganism contained in complex microorganism preparations of the present invention so that dirty
Debirs clearance in water is higher, decomposes more thorough.Wherein, each microorganism fungus kind is in metabolic debirs
During, various kinds of cell excision enzyme is secreted out of, effectively decompose various larger molecular organicses, such as cellulose, starch, albumen
Matter, grease and arene pollutant, eliminate stench.The present invention is compared with prior art, and its technological progress is obvious.
The present invention can rapidly decompose debirs, effectively suppress harmful microbe growth, new using microorganism in input polluted-water
Old metabolic activity, reduces water pollutant concentration, can be obviously improved the smelly problem of water body nigrescence, reduces Water quality ammonia nitrogen and contains with total phosphorus
The harmful substances such as amount, decomposing hydrogen sulfide, improve water body environment；Water transparency is significantly improved, detergent power stable with microbial inoculum
By force, the advantage for prepare simple, low cost, being suitable for river regulation, is effectively improved Colority of Sewage Water, turbidity, eliminates stench, and river course is controlled
Reason has great economical and environmentally friendly meaning.
Specific embodiment
Below by specific embodiment, the present invention is described further.
Embodiment 1
It is a kind of to be for a kind of preparation process of the complex microorganism preparations administered for river course organic matter：
（1）The independent Amplification Culture of each microorganism：
Pseudomonad：Pseudomonad on pseudomonad culture medium, 28-30 DEG C, inclined-plane culture first, then secondary seed solution training
Support, mixing fermentation culture reaches 1-2 × 108/mL to bacterium is dense.Described pseudomonad culture medium：Peptone 5g, yeast peptone
1g, agar powder 20g, distilled water 1000mL, PH=7.8.
Vibrios：On vibrios culture medium, 28-30 DEG C, inclined-plane culture, then secondary seed solution culture first mixes vibrios
Fermented and cultured reaches 1-2 × 108/mL to bacterium is dense.Described vibrios culture medium：Peptone 5g, yeast peptone 1g, agar powder 20g,
Distilled water 1000mL, PH=7.8.
Flavobacterium：Flavobacterium on Flavobacterium culture medium, 28-30 DEG C, inclined-plane culture first, then secondary seed solution training
Support, mixing fermentation culture reaches 1-2 × 108/mL to bacterium is dense.Described Flavobacterium culture medium：Peptone 5g, yeast peptone 1g,
Agar powder 20g, distilled water 1000mL, PH=7.8.
The pure coccus of nitrous acid：The pure coccus of nitrous acid on nitrite bacteria culture medium, 28-30 DEG C, inclined-plane culture first, so
Secondary seed solution culture afterwards, mixing fermentation culture reaches 1-2 × 108/mL to bacterium is dense.Described nitrococcus culture medium：Sulphur
Sour ammonium 0.5g, sodium chloride 0.3g, ferrous sulfate 0.03g, dipotassium hydrogen phosphate 1g, magnesium sulfate 0.03g, calcium chloride 7.5g, distilled water
1000mL、PH=8。
Thiobacillus thiooxidant：On thiobacillus thiooxidant is cultivated in thiosulfate solid medium, inclined-plane culture first, then
Secondary seed solution culture, mixing fermentation culture to bacterium it is dense reach 1-2 × 108/.Thiosulfate solid medium：Peptone
10g, dusty yeast 5g, bovine bile powder 8g, sodium citrate 10g, sodium thiosulfate 10g, ironic citrate 1g, bromothymol blue
0.04g, thymol blue 0.04g;
Bacillus subtilis：Bacillus subtilis 28-30 DEG C, makees primary inclined plane culture, so on beef-protein medium
After be inoculated into triangular flask do vibration secondary liquid culture, reach 3-4 × 108/mL to bacterium number.
Bacillus licheniformis：Bacillus licheniformis 28-30 DEG C, make primary inclined plane training on beef-protein medium
Support, be then seeded into triangular flask and do vibration secondary liquid culture, to bacterium number 3-4 × 108/mL is reached.
Bacillus aneurinolytieus：Bacillus aneurinolytieus 28-30 DEG C, makees one on beef-protein medium
Level inclined-plane culture, is then seeded into triangular flask and does vibration secondary liquid culture, and to bacterium number 3-4 × 108/mL is reached.
Saccharomycete：Saccharomycete 28-30 DEG C, makees primary inclined plane culture, then on lactic acid-Potato-dextrose culture medium
Be inoculated into triangular flask do vibration secondary liquid culture to bacterium number reach 1.0-2.5 × 108/mL.
Denitrogenation coccus：Denitrogenation coccus 28-30 DEG C, makees primary inclined plane culture on denitrogenation coccus culture medium, is then seeded into
Vibration secondary liquid culture to bacterium number is done in triangular flask and reaches 3-4 × 108/mL.Described denitrogenation coccus culture medium：Nitrous acid
Sodium 1g, magnesium sulfate 0.03g, manganese sulfate 0.01g, dipotassium hydrogen phosphate 0.75g, sodium carbonate 1g, sodium dihydrogen phosphate 0.25g, distilled water
1000mL、PH=7.2；.
Actinomyces：Actinomyces 28-30 DEG C, make primary inclined plane culture on Gause I culture medium, are then seeded into triangle
Vibration secondary liquid culture to bacterium number is done in bottle and reaches 3-4 × 108/mL.
（2）Make freeze-dried powder
The each raw material of the complex microorganism is prepared into respectively zymotic fluid, after mixing according to mass ratio, according to this area routine side
Method makes freeze-dried powder.
Embodiment 2
A kind of complex microorganism preparations administered for river course organic matter, including complex microorganism pseudomonad, vibrios, yellow bar
The pure coccus of bacterium, nitrous acid, thiobacillus thiooxidant, bacillus subtilis, bacillus licheniformis, bacillus aneurinolytieus, saccharomycete,
Denitrogenation coccus, actinomyces, described complex microorganism is made up of the raw material of following percentage by weight：Pseudomonad 15%, vibrios
10%th, the pure coccus 15% of Flavobacterium 10%, nitrous acid, thiobacillus thiooxidant 13%, bacillus subtilis 10%, bacillus licheniformis 5%, solution
Thiamine bacillus 5%, saccharomycete 7%, denitrogenation coccus 5%, actinomyces 5%.The above-mentioned bacterial classification for being adopted for this area routine
Bacterial classification, can commercially, or from China General Microbiological culture presevation administrative center（CGMCC）Purchase.Compound
In microorganism formulation, total bacteria count is mixed with freeze-dried powder, microbial inoculum or microbial inoculum and solid adjuvant material at 1,000,000,000 more than per gram
In the form of compound.Described solid adjuvant material is zeolite powder（40-80 mesh）, the ratio of zeolite powder and complex microorganism is 1：4
Obtain microbial inoculum and solid adjuvant material mixture.
Various enzymes and the antibiotic such as the complex microorganism preparations energy extracellular proteinase that the present invention is used, make to be accumulated in water body
Abundant residues bait, discharged waste, that plant and animal residues and pernicious gas (ammonia, hydrogen sulfide etc.) are first decomposed into small molecule is (many
Peptide, higher fatty acids etc.), after be decomposed into the organic matter (amino acid, lower fatty acid, monose, disaccharides etc.) of more small molecule, finally
Carbon dioxide, nitrate and sulfate etc. are decomposed into, COD, BOD in water is effectively reduced, the ammonia nitrogen (NH3- in water body is made
N), nitroso nitrogen (NO2-N) and sulfide concentration are reduced, so as to effectively improve water quality.
Embodiment 3
Jinan, Shandong Province river course, is affected by the discharge of neighbouring sanitary sewage throughout the year.COD is 32mg/L or so, and BOD is 8mg/L
Left and right, ammonia nitrogen concentration is 1.7mg/L or so, and sulfide concentration is 0.6 mg/L or so, and PH is 8-10, and water body blacks smelly, thoroughly
Lightness only has 25cm.Take a kind of 10 tons of complex microorganism systems for river regulation applied described in the present embodiment of river water quality
Agent, after river dilution, uniformly splashes in water body, and after applying 30 days, COD is 23mg/L or so, and BOD is 5mg/L or so, transparent
Degree brings up to 50cm；Dissolved oxygen rises to 4mg/L from 1.5mg/L, and ammonia nitrogen drops to 0.85mg/L, and sulfide concentration is dropped to
0.35 mg/L or so, PH are stable in 6.5-7.5；After 60 days, COD is 17mg/L or so, and BOD is 3mg/L or so, and transparency is carried
Height arrives 80cm；Dissolved oxygen rises to 6.2mg/L from 4mg/L, and ammonia nitrogen drops to 0.65mg/L, and sulfide concentration drops to 0.2
Mg/L or so, PH are stable in 7-7.5, reach《Water environment quality standard》GB3838-2002》In five class water levels, water
Structural reform is kind clearly.
The present invention can adopt naphthodiamide AAS or other common detection methods to the biological water of aquatic
Matter nitrite number is measured；PH value can be measured using pH indicator, pH precision test papers or other pH method of testings；
Ammonia nitrogen value can be measured using Berthelot spectrophotometry or other detection methods.
It should be noted last that, above example is only unrestricted to illustrate implementer's case of this material, to the greatest extent
Pipe has been described in detail with reference to preferred embodiment to the present invention, it will be understood by those within the art that, can be to this
The technical scheme of invention is modified or equivalent, and without deviating from the spirit and scope of technical solution of the present invention, it all should
Cover in the middle of scope of the presently claimed invention.
Claims (6)
1. it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that it is described including complex microorganism
Complex microorganism is made up of the raw material of following percentage by weight：It is 10 ~ 20% that the bacterium number of pseudomonad accounts for the percentage of total bacteria count,
It is 10 ~ 15% that the bacterium number of vibrios accounts for the percentage of total bacteria count, and it is 10 ~ 15% that the bacterium number of Flavobacterium accounts for the percentage of total bacteria count, nitrous
It is 10 ~ 15% that the bacterium number of the pure coccus of acid accounts for the percentage of total bacteria count, the bacterium number of thiobacillus thiooxidant account for the percentage of total bacteria count be 10 ~
15%, it is 10 ~ 20% that the bacterium number of bacillus subtilis accounts for the percentage of total bacteria count, and the bacterium number of bacillus licheniformis accounts for the hundred of total bacteria count
Point than being 5 ~ 15%, it is 5 ~ 10% that the bacterium number of bacillus aneurinolytieus accounts for the percentage of total bacteria count, and saccharomycetic bacterium number accounts for total bacterium
Several percentage is 5 ~ 10%, and it is 5 ~ 10% that the bacterium number of denitrogenation coccus accounts for the percentage of total bacteria count, and actinomycetic bacterium number accounts for total bacteria count
Percentage be 5 ~ 10%.
2. it is according to claim 1 it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that：Institute
The complex microorganism stated is made up of the raw material of following percentage by weight：Pseudomonad 15%, vibrios 10%, Flavobacterium 10%, nitrous acid
Pure coccus 15%, thiobacillus thiooxidant 13%, bacillus subtilis 10%, bacillus licheniformis 5%, bacillus aneurinolytieus 5%, ferment
Female bacterium 7%, denitrogenation coccus 5%, actinomyces 5%.
3. it is according to claim 1 it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that：
In described complex microorganism preparations, described total bacteria count is at 1,000,000,000 more than per gram.
4. it is according to claim 1 it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that：Institute
The complex microorganism preparations stated are in the form of freeze-dried powder, microbial inoculum or microbial inoculum and solid adjuvant material mixture.
5. it is according to claim 4 it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that：Institute
The solid adjuvant material stated is zeolite powder（40-80 mesh）, the ratio of zeolite powder and complex microorganism is 1：4 obtain microbial inoculum and consolidate
Body auxiliary material mixture.
6. it is according to claim 1 it is a kind of for river course organic matter administer complex microorganism preparations, it is characterised in that：Institute
Above-mentioned bacterial classification employed in the complex microorganism preparations stated for this area conventional bacterial classification, can commercially, or
From China General Microbiological culture presevation administrative center（CGMCC）Purchase.
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Cited By (6)
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| CN108178334A (en) * | 2018-01-15 | 2018-06-19 | 上海山恒生态科技股份有限公司 | A kind of black smelly complex microorganism preparations of quick elimination water body |
| CN108358322A (en) * | 2018-02-07 | 2018-08-03 | 山东九生物科技股份有限公司 | A kind of strain formula and preparation method thereof for administering black and odorous water |
| CN109133361A (en) * | 2017-06-27 | 2019-01-04 | 扬州四代实业有限公司 | A kind of biological slow-released ball and preparation method and application for black smelly river improvement |
| CN110028166A (en) * | 2019-03-12 | 2019-07-19 | 宜兴国际环保城科技发展有限公司 | A kind of novel C OD remover |
| CN111534463A (en) * | 2020-05-06 | 2020-08-14 | 河南林泉环保科技有限公司 | Composite microbial preparation for river treatment |
| CN113185072A (en) * | 2021-04-30 | 2021-07-30 | 江苏若焱环境设计有限公司 | River channel ecological restoration method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109133361A (en) * | 2017-06-27 | 2019-01-04 | 扬州四代实业有限公司 | A kind of biological slow-released ball and preparation method and application for black smelly river improvement |
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| CN113185072A (en) * | 2021-04-30 | 2021-07-30 | 江苏若焱环境设计有限公司 | River channel ecological restoration method |
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Application publication date: 20170510 |