CN107384833A - A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of administering method of black-odor riverway - Google Patents
A kind of complex microorganism combines microbial inoculum, its preparation method and application, a kind of administering method of black-odor riverway Download PDFInfo
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Abstract
The invention discloses a kind of complex microorganism to combine microbial inoculum, its preparation method and application, a kind of administering method of black-odor riverway.The complex microorganism combination microbial inoculum includes microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ and combines three kinds of microbial inoculum combinations, and each strain in the microbial inoculum A combinations is respectively bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans；Each strain in the microbial inoculum B combinations is respectively bacillus coagulans, silicate bacteria, bacillus firmus, lactic acid bacteria；Each strain in the microbial inoculum γ combinations is respectively lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus coagulans.Complex microorganism combination microbial inoculum can be used for the improvement of black-odor riverway, and it is administered, and the cycle is short, can effectively solve the odorous gas hydrogen sulfide in the exceeded water body with elimination of nitrogen phosphorus of black and odorous water.
Description
Technical field
The invention belongs to field of sewage treatment, and in particular to a kind of complex microorganism combines microbial inoculum, its preparation method and should
With a kind of, administering method of black-odor riverway.
Background technology
The urban water-bodies such as river are the important components of urban ecological system, it has, and environmental capacity of water is small, water body from
Net ability, the features such as easily polluting, and frequently as the important blowdown in city incity, dirty passage of receiving, water quality is easier to deteriorate.It is a large amount of dirty
The inflow of dye thing causes urban water-body eutrophication serious, causes the drastically consumption of Dissolved Oxygen in Water, causes water body long term hypoxia
So as to which black smelly state be presented.The urban water-bodies such as river occur black smelly, it has also become the universal phenomenon in China city, have a strong impact on bank
Resident living and city image.
The urban water-bodies such as river it is black it is smelly receive dirt mainly due to excessive and cause water ecology unbalance, water hypoxia or even anaerobism
Under the conditions of pollutant convert and produce ammonia nitrogen, the black such as odorant and iron, manganese sulfide such as hydrogen sulfide, VFA
Material.
At present, the administering method of city black and odorous water has Physical, chemical method and bioanalysis.Wherein, in bioanalysis usually
Microbe preparation administers city black and odorous water, and a certain amount of microorganism formulation is added into water body, accelerates to pollute in water body
Thing is degraded, and strengthens the self-purification function of water body.
Single microorganism preparation is usually present in the application process for administering black and odorous water to be polluted to nitrogen, phosphorus and organic matter etc.
The problems such as degradation capability of thing is weak, treatment effeciency is low, and the elimination to odorous gas hydrogen sulfide in black and odorous water acts on unobvious.
The content of the invention
In order to solve the above technical problems, the invention provides a kind of complex microorganism combine microbial inoculum, its preparation method and should
With a kind of, administering method of black-odor riverway.Microbial inoculum A combinations, microbial inoculum B combinations and bacterium are included in the complex microorganism combination microbial inoculum
Agent γ combines three kinds of microbial inoculum combinations, improvement of the microbial inoculum combination available for black-odor riverway, can effectively reduce the black and odorous water
In the pollutant component such as nitrogen, phosphorus and organic matter, while there is obvious eliminate to make to odorous gas hydrogen sulfide in the black and odorous water
With so as to reach the quick purpose for improving black-odor riverway water quality, recovering water ecology balance.
The technical scheme that the present invention takes is：
A kind of complex microorganism combines microbial inoculum, including microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations；
The microbial inoculum A combinations include bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans；
The microbial inoculum B combinations include bacillus coagulans, silicate bacteria, bacillus firmus and lactic acid bacteria；
The microbial inoculum γ combinations include lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and condense gemma
Bacillus.
The weight ratio of each strain in the microbial inoculum A combinations is bacillus coagulans：Lactic acid bacteria：Saccharomycete：Aspergillus sojae：
Thiobacillus denitrificans=(2.7~3.3)：(1.8~2.2)：(0.45~0.55)：(1.35~1.65)：(2.7~3.3).
The weight ratio of each strain in the microbial inoculum B combinations is bacillus coagulans：Silicate bacteria：Hard gemma bar
Bacterium：Lactic acid bacteria=(4.5~5.5)：(94.5~110.5)：(94.5~110.5)：(1.8~2.2).
The weight ratio of each strain in the microbial inoculum γ combinations is lactic acid bacteria：Bacillus subtilis：Saccharomycete：Lichens gemma
Bacillus：Bacillus coagulans=(3.15~3.85)：(1.8~2.2)：(0.45~0.55)：(1.35~1.65)：(2.25~
2.75)。
In the complex microorganism combination microbial inoculum, the weight ratio of microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations is
1：1：1.
The preparation method of each microbial inoculum combination comprises the following steps：
(1) each strain is inoculated in 36~48h of fluid nutrient medium culture respectively；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, volume production 36~48h of culture, and strain is trained with fermentation
The weight ratio for supporting base is 1：9~15；
(3) weight proportion of the strain during the strain after step (2) volume production culture is combined according to each microbial inoculum mixes, and intersects
Antagonism 36~48h of culture, it is 4~9 × 10 to viable count10Cfu/ml, microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ are obtained respectively
Combination.Mixed bacteria is 1 with the weight ratio for intersecting antagonism culture medium：15~20.
Logical filtrated air is stirred in the step (2), in fermentation process for every eight hours 2 minutes, the rotating speed of stirring is 70rpm/
min.The flow of the filtrated air is 90L/min.
The preparation method of microbial inoculum A combination is：
(1) bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans are inoculated in liquid training respectively
Support base, 28~32 DEG C of culture 48h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 7.2~
8.8g, 9~11g of peptone, 2.7~3.3g of yeast extract, 4.5~5.5g of sodium chloride, 0.9~1.1g of potassium dihydrogen phosphate, micro member
0.09~0.11g of element, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；The trace element is Mn2+、Mg2 +Or Fe2+, used is their sulfate；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 48h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：9~15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef
7.2~8.8g of cream, 9~11g of peptone, 2.7~3.3g of yeast extract, 4.5~5.5g of sodium chloride, 0.9~1.1g of potassium dihydrogen phosphate,
0.09~0.11g of trace element, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；The trace element is
Mn2+、Mg2+Or Fe2+, used is their sulfate；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Lactic acid bacteria：Saccharomycete：Soy sauce
Aspergillus：Thiobacillus denitrificans=(2.7~3.3)：(1.8~2.2)：(0.45~0.55)：(1.35~1.65)：(2.7~3.3) mix
Close, intersect antagonism culture 48h, logical filtrated air 2 minutes, speed of agitator 70rpm/min, institute are stirred for every eight hours in incubation
The flow for stating filtrated air is 90L/min, and mixed bacteria is 1 with the weight ratio for intersecting antagonism culture medium：15~20, obtain bacterium
Agent A is combined, and its viable count is 4~6 × 1010cfu/ml.The antagonism culture medium that intersects is the composition contained in every liter of culture medium
It is as follows：7.2~8.8g of beef extract, 9~11g of peptone, 2.7~3.3g of yeast extract, 4.5~5.5g of sodium chloride, potassium dihydrogen phosphate
0.9~1.1g, 0.09~0.11g of trace element, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；It is described
Trace element is Mn2+、Mg2+Or Fe2+, used is their sulfate.
The preparation method of microbial inoculum B combination is：
(1) bacillus coagulans, silicate bacteria, bacillus firmus and lactic acid bacteria are inoculated in Liquid Culture respectively
Base, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 7.2~
8.8g, 9~11g of peptone, 3.6~4.4g of yeast extract, calcium carbonate 1.53g~1.87g, 18~22g of glucose, Tween 80 0.9
~1.1ml, 0.9~1.1g of potassium dihydrogen phosphate, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：9~15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef
7.2~8.8g of cream, 9~11g of peptone, 3.6~4.4g of yeast extract, calcium carbonate 1.53g~1.87g, 18~22g of glucose, tell
80 0.9~1.1ml of temperature, 0.9~1.1g of potassium dihydrogen phosphate, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~
7.2；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Silicate bacteria：Hard bud
Spore bacillus：Lactic acid bacteria=(4.5~5.5):(94.5~110.5):(94.5~110.5):(1.8~2.2) mix, intersect antagonism
36h is cultivated, mixed bacteria is 1 with the weight ratio for intersecting antagonism culture medium：15~20, microbial inoculum B combinations are obtained, its viable count is
7~9 × 1010cfu/ml.The antagonism culture medium that intersects is that the composition contained in every liter of culture medium is as follows：Beef extract 7.2~
8.8g, 9~11g of peptone, 3.6~4.4g of yeast extract, calcium carbonate 1.53g~1.87g, 18~22g of glucose, Tween 80 0.9
~1.1ml, 0.9~1.1g of potassium dihydrogen phosphate, 27~33g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2.
The preparation method of microbial inoculum γ combination is：
(1) lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus coagulans are inoculated in respectively
Fluid nutrient medium, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract
7.2~8.8g, 2.7~3.3g of peptone, 3.15~3.85g of yeast extract, 0.9~1.1ml of Tween 80, epsom salt 1.35
~1.65g, 18~22g of glucose, 45~55g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：9~15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef
7.2~8.8g of cream, 2.7~3.3g of peptone, 3.15~3.85g of yeast extract, 0.9~1.1ml of Tween 80, epsom salt
1.35~1.65g, 18~22g of glucose, 45~55g of sucrose, remaining be distilled water, acid-base value is 6.8~7.2；
(3) strain after step (2) volume production culture is compared into lactic acid bacteria according to weight：Bacillus subtilis：Saccharomycete：Lichens
Bacillus：Bacillus coagulans=(3.15~3.85)：(1.8~2.2)：(0.45~0.55)：(1.35~1.65)：
(2.25~2.75) mix, intersect antagonism culture 36h, mixed bacteria is 1 with the weight ratio for intersecting antagonism culture medium：15~
20, microbial inoculum γ combinations are obtained, its viable count is 4~7 × 1010cfu/ml.The intersection antagonism culture medium is in every liter of culture medium
The composition contained is as follows：7.2~8.8g of beef extract, 2.7~3.3g of peptone, 3.15~3.85g of yeast extract, Tween 80 0.9~
1.1ml, 1.35~1.65g of epsom salt, 18~22g of glucose, 45~55g of sucrose, remaining be distilled water, acid-base value is
6.8~7.2.
Present invention also offers application of the complex microorganism combination microbial inoculum in black-odor riverway improvement.
A kind of administering method of black-odor riverway, the administering method comprise the following steps：
A, the pollution of the external source of black-odor riverway is controlled, carries out and cut a dirt and receive pipe work；
B, microbial inoculum A is combined and microbial inoculum B combined hybrids, launched in black-odor riverway；
C, after 5~6 months, microbial inoculum γ combinations is launched in river course, put aquatic animals and plants in a suitable place to breed, recover aquatic ecosystem
Recover water body self-purification ability；
D, long term maintenance is carried out to river course afterwards.
The injected volume of the microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations is 20~50ppm.This launches dosage
In the range of, strain can be got up with fast-growth, with flora advantage suppress harmful bacteria growth, can quick decomposing pollutant, enter
And make it that effect is quickly obvious.Launch the very few growth breeding of dosage and do not have flora advantage slowly, regulation effect is undesirable, throws
Let off more as the effect for launching this dosage, therefore dispensing can excessively cause to waste.
The function of each strain in complex microorganism combination microbial inoculum disclosed by the invention is as follows：
Bacillus coagulans：Secreting amylase and protease, effectively suppress the growth of harmful bacteria；
Lactic acid bacteria：Protein in water is decomposed, nutrition is provided for aquatic eastern plant；
Saccharomycete：Decompose the polysaccharide such as starch in water body；
Aspergillus sojae：Heavy metal is absorbed, phosphorus decomposing, nutrition is provided for water plant；
Thiobacillus denitrificans：Sulfide is converted into sulfate or elemental sulfur, by nitrate, Nitrite transformation is nitrogen；
Silicate bacteria：Slightly solubility potassium and phosphorus are dissolved out and utilized in itself for crop and thalline, while produces hormone, ammonia
The materials such as base acid, polysaccharide promote the growth of plant；
Bacillus firmus：Secreting amylase and protease, effectively suppress the growth of harmful bacteria；
Bacillus subtilis：Secreting amylase, lipase, protease, cellulase；
Bacillus licheniformis：Decompose organic matter, secreting amylase.
Microbial inoculum A combination be by bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans by weight
For (2.7~3.3):(1.8~2.2):(0.45~0.55):(1.35~1.65):(2.7~3.3) intersect antagonism culture to obtain,
These strain collective effects so that microbial inoculum A combinations being capable of the black sordes matter such as ammonia, methyl mercaptan, hydrogen sulfide in fast decoupled water.
Microbial inoculum B combinations are to be by weight by bacillus coagulans, silicate bacteria, bacillus firmus, lactic acid bacteria
(4.5~5.5):(94.5~110.5):(94.5~110.5):(1.8~2.2) intersect antagonism culture to obtain, these strains are total to
Same-action so that microbial inoculum B combinations can ammonia nitrogen substances in rapid conversion degradation water, body eutrophication material can be removed, changed
Kind river water quality and microflora.
Microbial inoculum γ combination be by lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis, bacillus coagulans by
Weight ratio is (3.15~3.85):(1.8~2.2):(0.45~0.55):(1.35~1.65):(2.25~2.75) intersect short of money
Anti- culture obtains, these strain collective effects so that microbial inoculum γ combinations have regulation water PH value, adjustment microorganisms in water ring
Border, the effect for improving water transparency, can recover water ecology, to after being repaired through microbial inoculum A combinations and microbial inoculum B combinations
While microbial inoculum γ combination is launched in black and odorous water, put in a suitable place to breed aquatic animals and plants water body is regulated and controled, monitored, conditioning system each group
Into unit, so as to optimize river course index, Balance Water Quality and the harmony stabilization for keeping each biological flora, the biology for increasing system is more
Sample, recover aquatic ecosystem, recover water body self-purification ability.
More than each strain in the combination of each microbial inoculum dosage be required to be strict controlled in it is disclosed by the invention within the scope of, this
In the range of can make between each strain that both mutual antagonism and can syntrophism was not in the dead situation of single strain, so as to influence
Using effect.Because antagonism promotes thalline to secrete more more effective enzymes faster in incubation is determined, make its effect will
Much larger than single culture.
In the governance process of black-odor riverway, first water body environment, abatement river are repaired using microbial inoculum A combinations and microbial inoculum B combinations
The silt depth at bottom, reach requirement in water body indices and then launch microbial inoculum γ combinations, to recover the ecology in water body
Balance.
Compared with prior art, the present invention possesses advantages below：
1. being cultivated in every kind of microbial inoculum combination during each Spawn incubation using unified culture medium, ensureing that strain is optimal
On the premise of Reproduction Conditions, simplify the preparation process of microbial inoculum combination, save cost；
2. every kind of microbial inoculum combination forms by a variety of strains, each strain plays synergy, quick shape in sewage disposal
Into dominant microflora, all kinds of eutrophy of fast decoupled black and odorous water, peculiar smell is eliminated, harmful substance of degrading；
3. the combination of these three microbial inoculums is combined to administer the dirt of black-odor riverway using microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ
Dye problem, it is administered, and cycle is short, and the nitrogen phosphorus that can effectively solve black and odorous water is exceeded, the too high problem of COD value, can effectively eliminate water body
In odorous gas hydrogen sulfide, cut down bed-silt thickness；
4. after the water quality of black-odor riverway is repaired in microbial inoculum A combinations and microbial inoculum B combined hybrids, microbial inoculum γ combinations are launched to put down
Flora in Hengshui, improve aquatic environment, recover the ecological balance in water body；
5. the present invention first uses microbial treatment water body, after water body administered by the self-purification capacity of water body, reach and take stopgap measures
The purpose effected a permanent cure, secondary pollution will not be produced；
6. there is the features such as safe efficient, cost is low, reduced investment, simple to operate.
Embodiment
All strains disclosed by the invention are through being commercially available.
Embodiment 1
A kind of complex microorganism combines microbial inoculum, including microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations；
The preparation method of microbial inoculum A combination is：
(1) bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans are inoculated in liquid training respectively
Support base, 28~32 DEG C of culture 48h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8g, albumen
Peptone 10g, yeast extract 3g, sodium chloride 5g, potassium dihydrogen phosphate 1g, micro- 0.1g, sucrose 30g, remaining be distilled water, acid-base value
For 6.8~7.2；The trace element is Mn2+、Mg2+Or Fe2+, used is their sulfate；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 48h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：10；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8g, protein 10 g, yeast extract 3g, sodium chloride 5g, potassium dihydrogen phosphate 1g, micro- 0.1g, sucrose 30g, remaining be distilled water,
Acid-base value is 6.8~7.2；The trace element is Mn2+、Mg2+Or Fe2+, used is their sulfate；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Lactic acid bacteria：Saccharomycete：Soy sauce
Aspergillus：Thiobacillus denitrificans=3：2：0.5：1.5：3 mixing, intersect antagonism culture 48h, logical nothing is stirred for every eight hours in incubation
Bacterium air 2 minutes, speed of agitator 70rpm/min, the flow of the filtrated air is 90L/min, and mixed bacteria is with intersecting antagonism
The weight ratio of culture medium is 1：15, microbial inoculum A combinations are obtained, its viable count is 5 × 1010cfu/ml.The intersection antagonism culture
Base is that the composition contained in every liter of culture medium is as follows：Beef extract 8g, peptone 10g, yeast extract 3g, sodium chloride 5g, biphosphate
Potassium 1g, micro- 0.1g, sucrose 30g, remaining be distilled water, acid-base value is 6.8~7.2；The trace element is Mn2+、Mg2 +Or Fe2+, used is their sulfate.
The preparation method of microbial inoculum B combination is：
(1) bacillus coagulans, silicate bacteria, bacillus firmus and lactic acid bacteria are inoculated in Liquid Culture respectively
Base, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8g, peptone
10g, yeast extract 4g, calcium carbonate 1.7g, glucose 20g, Tween 80 1.0ml, potassium dihydrogen phosphate 1.0g, sucrose 30g, remaining be
Distilled water, acid-base value are 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：10；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8g, peptone 10g, yeast extract 4g, calcium carbonate 1.7g, glucose 20g, Tween 80 1.0ml, potassium dihydrogen phosphate 1.0g, sucrose
30g, remaining be distilled water, acid-base value is 6.8~7.2；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Silicate bacteria：Hard bud
Spore bacillus：Lactic acid bacteria=5：105：105：2 mixing, intersect antagonism culture 36h, mixed bacteria and the weight for intersecting antagonism culture medium
The ratio between be 1：15, microbial inoculum B combinations are obtained, its viable count is 8 × 1010cfu/ml.The intersection antagonism culture medium is every liter of culture
The composition contained in base is as follows：Beef extract 8g, peptone 10g, yeast extract 4g, calcium carbonate 1.7g, glucose 20g, Tween 80
1.0ml, potassium dihydrogen phosphate 1.0g, sucrose 30g, remaining be distilled water, acid-base value is 6.8~7.2.
The preparation method of microbial inoculum γ combination is：
(1) lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus coagulans are inoculated in respectively
Fluid nutrient medium, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8g, peptone 3g, yeast extract 3.5g, Tween 80 1.0ml, epsom salt 1.5g, glucose 20g, sucrose 50g, remaining for steam
Distilled water, acid-base value are 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：10；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8g, peptone 3g, yeast extract 3.5g, Tween 80 1.0ml, epsom salt 1.5g, glucose 20g, sucrose 50g, remaining for steam
Distilled water, acid-base value are 6.8~7.2；
(3) strain after step (2) volume production culture is compared into lactic acid bacteria according to weight：Bacillus subtilis：Saccharomycete：Lichens
Bacillus：Bacillus coagulans=3.5：2：0.5：1.5：2.5 mixing, intersect antagonism culture 36h, and mixed bacteria is short of money with intersecting
The weight ratio of anti-culture medium is 1：15, microbial inoculum γ combinations are obtained, its viable count is 6 × 1010cfu/ml.The intersection antagonism training
It is that the composition contained in every liter of culture medium is as follows to support base：Beef extract 8g, peptone 3g, yeast extract 3.5g, Tween 80 1.0ml, seven
Water magnesium sulfate 1.5g, glucose 20g, sucrose 50g, remaining be distilled water, acid-base value is 6.8~7.2.
The complex microorganism combination microbial inoculum can be used for the improvement to black-odor riverway, reduces the ammonia-nitrogen content in water body, divides
All kinds of eutrophy of black and odorous water are solved, eliminate peculiar smell, harmful substance of degrading, the pollution problem different to water body carries out specific aim solution
Certainly.
Black-odor riverway to be administered using complex microorganism combination microbial inoculum, certain development zone river course is about 3000 meters,
Width about 15-35 rice, depth of water 1-3.5 rice, bed mud thickness 0.5-0.7 rice.For a long time due to a large amount of sanitary sewages and trade effluent
Be discharged into naturally, river seriously pollutes.River wawter bloom is serious, low in eutrophication, black and odorous water, transparency, mud hair
Black, river surface has more white garbage and greasy dirt.
Its administering method is as follows：
A, the pollution of the external source of black-odor riverway is controlled, carries out and cut a dirt and receive pipe work；
B, microbial inoculum A being combined and microbial inoculum B combined hybrids, launched in black-odor riverway, both dispensing dosage is 20~
50ppm；
C, after 6 months, by microbial inoculum γ combinations, according to dosage 20~50ppm is launched in river course, while puts aquatic dynamic plant in a suitable place to breed
Thing, regulated and controled, monitored, each component units of conditioning system, so as to optimize river course index, Balance Water Quality and each biological flora of holding
Harmony it is stable, increase the bio-diversity of system, recover aquatic ecosystem, recover water body self-purification ability.
D, long term maintenance is carried out to river course afterwards.
After microbial inoculum A combinations and microbial inoculum B combined hybrids input river course, the monitoring process in governance process is as follows：
First stage：After launching 48h, water surface and environment stink lower 50% or so.
Second stage：After launching 72h, creek bottom mud is under the microbial inoculum A combinations and the effect of microbial inoculum B combined hybrids of the present invention
Float after fermentation, floatation surface on river surge silt layer, into water body and bottom sediment odor eliminates and the silt displacement phase.According to creek
Silt depth and streamflow regime, the stage length is variant, general 14~20 days.
Phase III：Launch 21~62 days, win initial success, black smelly gradually elimination, riverbed water level deepen, and water transparency carries
, there is more microorganisms in height, and the life condition of aquatic animal is improved.
Fourth stage：Launch 63~90 days, water quality is clearly better, and various water plants start to breed, every water quality index
Change 60% or so, bed mud reduce 20cm or so.
5th stage：Launch 6 months, cut down 30~40cm of mud, water quality is significantly improved.
Each index result in river regulation different phase, water body is as shown in table 1：
Each index result in the river regulation different phase water body of table 1
As it can be seen from table 1 combining the microbial inoculum improvement of 6 months by complex microorganism, the COD value in water body reduces
91.7%, NH3- N content reduces 88.9%, and for total phosphorus content by low 92.2%, river water quality has reached national surface water IV
Class water standard.
Embodiment 2
A kind of complex microorganism combines microbial inoculum, including microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations；
The preparation method of microbial inoculum A combination is：
(1) bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans are inoculated in liquid training respectively
Support base, 28~32 DEG C of culture 48h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8.8g, egg
White peptone 11g, yeast extract 3.3g, sodium chloride 5.5g, potassium dihydrogen phosphate 1.1g, micro- 0.11g, sucrose 33g, remaining be distillation
Water, acid-base value are 6.8~7.2；The trace element is Mn2+、Mg2+Or Fe2+, used is their sulfate；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 48h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8.8g, peptone 11g, yeast extract 3.3g, sodium chloride 5.5g, potassium dihydrogen phosphate 1.1g, micro- 0.11g, sucrose 33g, its
Remaining is distilled water, and acid-base value is 6.8~7.2；The trace element is Mn2+、Mg2+Or Fe2+, used is them
Sulfate；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Lactic acid bacteria：Saccharomycete：Soy sauce
Aspergillus：Thiobacillus denitrificans=2.7：1.8：0.55：1.65：3.3 mix, intersection antagonism culture 48h, in incubation for every eight hours
The logical filtrated air 2 minutes, speed of agitator 70rpm/min of stirring, the flow of the filtrated air be 90L/min, mixed bacteria and
The weight ratio for intersecting antagonism culture medium is 1：20, microbial inoculum A combinations are obtained, its viable count is 6 × 1010cfu/ml.The intersection
Antagonism culture medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8.8g, peptone 11g, yeast extract 3.3g, sodium chloride
5.5g, potassium dihydrogen phosphate 1.1g, micro- 0.11g, sucrose 33g, remaining be distilled water, acid-base value is 6.8~7.2；It is described
Trace element is Mn2+、Mg2+Or Fe2+, used is their sulfate.
The preparation method of microbial inoculum B combination is：
(1) bacillus coagulans, silicate bacteria, bacillus firmus and lactic acid bacteria are inoculated in Liquid Culture respectively
Base, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8.8g, albumen
Peptone 11g, yeast extract 4.4g, calcium carbonate 1.87g, glucose 22g, Tween 80 1.1ml, potassium dihydrogen phosphate 1.1g, sucrose 33g, its
Remaining is distilled water, and acid-base value is 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8.8g, peptone 11g, yeast extract 4.4g, calcium carbonate 1.87g, glucose 22g, Tween 80 1.1ml, potassium dihydrogen phosphate 1.1g,
Sucrose 33g, remaining be distilled water, acid-base value is 6.8~7.2；
(3) strain after step (2) volume production culture is compared into bacillus coagulans according to weight：Silicate bacteria：Hard bud
Spore bacillus：Lactic acid bacteria=5.5：110.5：110.5：2.2 mixing, intersect antagonism culture 36h, mixed bacteria is with intersecting antagonism culture
The weight ratio of base is 1：20, microbial inoculum B combinations are obtained, its viable count is 9 × 1010cfu/ml.It is described intersection antagonism culture medium be
The composition contained in every liter of culture medium is as follows：Beef extract 8.8g, peptone 11g, yeast extract 4.4g, calcium carbonate 1.87g, glucose
22g, Tween 80 1.1ml, potassium dihydrogen phosphate 1.1g, sucrose 33g, remaining be distilled water, acid-base value is 6.8~7.2.
The preparation method of microbial inoculum γ combination is：
(1) lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and bacillus coagulans are inoculated in respectively
Fluid nutrient medium, 28~32 DEG C of culture 36h；The fluid nutrient medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8.8g, peptone 3.3g, yeast extract 3.85g, Tween 80 1.1ml, epsom salt 1.65g, glucose 22g, sucrose 55g, its
Remaining is distilled water, and acid-base value is 6.8~7.2；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, 28~32 DEG C of volume production culture 36h, fermentation process
In stir logical filtrated air for every eight hours 2 minutes, the flow of the filtrated air be 90L/min, rotating speed 70rpm/min, strain and
The weight ratio of fermentation medium is 1：15；The fermentation medium is that the composition contained in every liter of culture medium is as follows：Beef extract
8.8g, peptone 3.3g, yeast extract 3.85g, Tween 80 1.1ml, epsom salt 1.65g, glucose 22g, sucrose 55g, its
Remaining is distilled water, and acid-base value is 6.8~7.2；
(3) strain after step (2) volume production culture is compared into lactic acid bacteria according to weight：Bacillus subtilis：Saccharomycete：Lichens
Bacillus：Bacillus coagulans=3.85：2.2：0.55：1.65：2.75 mixing, intersect antagonism culture 36h, mixed bacteria with
The weight ratio for intersecting antagonism culture medium is 1：20, microbial inoculum γ combinations are obtained, its viable count is 7 × 1010cfu/ml.The intersection
Antagonism culture medium is that the composition contained in every liter of culture medium is as follows：Beef extract 8.8g, peptone 3.3g, yeast extract 3.85g, tween
80 1.1ml, epsom salt 1.65g, glucose 22g, sucrose 55g, remaining be distilled water, acid-base value is 6.8~7.2.
The complex microorganism combination microbial inoculum can be used for the improvement to black-odor riverway, reduces the ammonia-nitrogen content in water body, divides
All kinds of eutrophy of black and odorous water are solved, eliminate peculiar smell, harmful substance of degrading, the pollution problem different to water body carries out specific aim solution
Certainly.
Black-odor riverway is administered using complex microorganism combination microbial inoculum, certain village's river regulation scope about 1500
Rice, wide about 2~6 meters, 1~3 meter of the depth of water, bed mud thickness 0.5-0.8 rice, total capacity is about 20000m3.This river is mainly with life
Based on sewage, a small number of trade effluents, which converge, shows as black, and wawter bloom is serious, and in eutrophication, transparency is extremely low, and mud, which blacks, to be dissipated
Send out putrefactive odor strong.
Its administering method is as follows：
A, the pollution of the external source of black-odor riverway is controlled, carries out and cut a dirt and receive pipe work；
B, microbial inoculum A being combined and microbial inoculum B combined hybrids, launched in black-odor riverway, both dispensing dosage is 20~
50ppm；
C, after 6 months, by microbial inoculum γ combinations, according to dosage 20~50ppm is launched in river course, while puts aquatic dynamic plant in a suitable place to breed
Thing, regulated and controled, monitored, each component units of conditioning system, so as to optimize river course index, Balance Water Quality and each biological flora of holding
Harmony it is stable, increase the bio-diversity of system, recover aquatic ecosystem, recover water body self-purification ability.
D, long term maintenance is carried out to river course afterwards.
After microbial inoculum A combinations and microbial inoculum B combined hybrids input river course, the monitoring process in governance process is as follows：
First stage：After launching 48h, water surface and environment stink lower 70% or so.
Second stage：After launching 72h, creek bottom mud is under the microbial inoculum A combinations and the effect of microbial inoculum B combined hybrids of the present invention
Float after fermentation, floatation surface on river surge silt layer, into water body and bottom sediment odor eliminates and the silt displacement phase.According to creek
Silt depth and streamflow regime, the stage length is variant, general 14~20 days.
Phase III：Launch 21~62 days, win initial success, black smelly gradually elimination, riverbed water level deepen, and water transparency carries
, there is more microorganisms in height, and the life condition of aquatic animal is improved.
Fourth stage：Launch 63~90 days, water quality is clearly better, and various water plants start to breed, every water quality index
Change 60% or so, bed mud reduce 20cm or so.
5th stage：Launch 6 months, cut down 30~40cm of mud, water quality is significantly improved.
Each index result in river regulation different phase, water body is as shown in table 2：
Each index result in the river regulation different phase water body of table 2
From table 2 it can be seen that combining the microbial inoculum improvement of 6 months by complex microorganism, the COD value in water body reduces
81.1%, NH3- N content reduces 94.3%, and for total phosphorus content by low 92.2%, river water quality has reached national surface water IV
Class water standard.
Comparative example 1
Other simply throw the mixing of microbial inoculum A combinations and microbial inoculum B combinations in the previous stage of river regulation with embodiment 1
Enter to replace with and only put into single microbial inoculum B combinations, it is 40~100ppm that it, which puts into dosage, and other conditions keep constant, in river course
Different phase is administered, each index result in water body is as shown in table 3：
Each index result in the river regulation different phase water body of table 3
From table 3 it can be seen that combining the improvement of 6 months by microbial inoculum B, the COD value in water body reduces 57.9%, NH3-
N content reduces 92.7%, and for total phosphorus content by low 72.8%, although river water quality has approached national surface water V classes water quality mark
Standard, but its regulation effect is far below the regulation effect after microbial inoculum A combinations and microbial inoculum B combined hybrids.
Comparative example 2
Other simply throw the mixing of microbial inoculum A combinations and microbial inoculum B combinations in the previous stage of river regulation with embodiment 2
Enter to replace with and only put into single microbial inoculum A combinations, it is 40~100ppm that it, which puts into dosage, and other conditions keep constant, in river course
Different phase is administered, each index result in water body is as shown in table 4：
Each index result in the river regulation different phase water body of table 4
From table 4, it can be seen that combining the improvement of 6 months by microbial inoculum A, the COD value in water body reduces 39.1%, NH3-
N content reduces 76.2%, and for total phosphorus content by low 78.1%, river water quality has approached national surface water V class water standards, but
It is that its regulation effect combines and the regulation effect after microbial inoculum B combined hybrids far below microbial inoculum A.
It is above-mentioned that microbial inoculum, its preparation method and application, a kind of black-odor riverway are combined to a kind of complex microorganism with reference to embodiment
Administering method carry out detailed description, be illustrative rather than limited, can be included according to limited scope some
Individual embodiment, therefore changing and modifications in the case where not departing from present general inventive concept, should belong within protection scope of the present invention.
Claims (10)
1. a kind of complex microorganism combines microbial inoculum, it is characterised in that the complex microorganism combination microbial inoculum include microbial inoculum A combinations,
Microbial inoculum B is combined and microbial inoculum γ combinations；
The microbial inoculum A combinations include bacillus coagulans, lactic acid bacteria, saccharomycete, Aspergillus sojae and thiobacillus denitrificans；
The microbial inoculum B combinations include bacillus coagulans, silicate bacteria, bacillus firmus and lactic acid bacteria；
The microbial inoculum γ combinations include lactic acid bacteria, bacillus subtilis, saccharomycete, bacillus licheniformis and condense gemma bar
Bacterium.
2. complex microorganism according to claim 1 combines microbial inoculum, it is characterised in that each bacterium in the microbial inoculum A combinations
The weight ratio of kind is bacillus coagulans：Lactic acid bacteria：Saccharomycete：Aspergillus sojae：Thiobacillus denitrificans=(2.7~3.3):(1.8~
2.2):(0.45~0.55):(1.35~1.65):(2.7~3.3).
3. complex microorganism according to claim 1 or 2 combines microbial inoculum, it is characterised in that each in the microbial inoculum B combinations
The weight ratio of strain is bacillus coagulans：Silicate bacteria：Bacillus firmus：Lactic acid bacteria=(4.5~5.5):(94.5~
110.5):(94.5~110.5):(1.8~2.2).
4. the complex microorganism combination microbial inoculum according to claim 1-3 any one, it is characterised in that the microbial inoculum γ groups
The weight ratio of each strain in conjunction is lactic acid bacteria：Bacillus subtilis：Saccharomycete：Bacillus licheniformis：Bacillus coagulans=
(3.15~3.85):(1.8~2.2):(0.45~0.55):(1.35~1.65):(2.25~2.75).
5. the complex microorganism combination microbial inoculum according to claim 1-4 any one, it is characterised in that the composite microbial
In thing combination microbial inoculum, the weight ratio of microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations is 1：1：1.
6. according to the complex micro organism fungicide described in claim 1-5 any one, it is characterised in that the preparation of each microbial inoculum combination
Method comprises the following steps：
(1) each strain is inoculated in 36~48h of fluid nutrient medium culture respectively；
(2) strain after step (1) is cultivated is respectively placed in fermentation tank, volume production 36~48h of culture, strain and fermentation medium
Weight ratio be 1：9~15；
(3) weight proportion of the strain during the strain after step (2) volume production culture is combined according to each microbial inoculum mixes, and intersects antagonism
36~48h is cultivated, is 4~9 × 10 to viable count10Cfu/ml, microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ groups are obtained respectively
Close.
7. complex micro organism fungicide according to claim 6, it is characterised in that every in fermentation process in the step (2)
The logical filtrated air of stirring in 8 hours 2 minutes, the rotating speed of stirring is 70rpm/min.
8. application of the complex microorganism combination microbial inoculum according to claim 1 in black-odor riverway improvement.
9. a kind of administering method of black-odor riverway, it is characterised in that the administering method comprises the following steps：
A, the pollution of the external source of black-odor riverway is controlled, carries out and cut a dirt and receive pipe work；
B, microbial inoculum A is combined and microbial inoculum B combined hybrids, launched in black-odor riverway；
C, after 5~6 months, microbial inoculum γ combinations is launched in river course, put aquatic animals and plants in a suitable place to breed, recovered aquatic ecosystem and recover
Water body self-purification ability；
D, long term maintenance is carried out to river course afterwards.
10. administering method according to claim 8, it is characterised in that microbial inoculum A combinations, microbial inoculum B combinations and microbial inoculum γ combinations
Injected volume be 20~50ppm.
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