KR101231977B1 - Biological processing method for removing green algae and offensive odor from a river or lakes and marshes - Google Patents

Abstract

The present invention Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens) to KS-4, Bacillus agar ladder lances (Bacillus agaradhaerens ) KS-5, Paenibacillus lentimorbus ) KS-6, Bacillus laevolacticus KS-7, Leuconostoc paramesenteroides KS-9, Kurthia sibirica KS-13 and Sphingobacterium Spiriborum-GC subgroup B (Flavobacterium ) ( Sphingobacterium spiritivorum-GC subgroup B (Flavobacterium) ) It is characterized by removing the algae and odor by incorporating a complex microbial agent containing KS-18 in the river or lake It provides a method of removing green algae and odor in rivers or lakes.

Description

BIOLOGICAL PROCESSING METHOD FOR REMOVING GREEN ALGAE AND OFFENSIVE ODOR FROM A RIVER OR LAKES AND MARSHES}

The present invention relates to a method for removing green algae and odors in a river or lake using a complex microbial agent, and more particularly, by simply flowing to the running water, the green algae breeding in a river or lake and various odors generated therefrom are simplified. The present invention relates to a method for removing green algae and odor in a river or lake using a removable microbial agent.

In general, organics and inorganics flowing from the surroundings of rivers and lakes are deposited on the river to generate green algae, which causes odors and damages nearby residents.

Primary water purification is required to improve water quality upstream of streams and lakes, and this usually requires special treatment equipment for sewage treatment, which results in a large amount of excess sludge. Such surplus sludge is usually landfilled to contaminate the environment, and furthermore, there is a lot of difficulties in disposal as marine dumping is prohibited.

Therefore, an effort to reduce the pollution of the green algae and various pollutants caused by the green algae and various pollutants in the downstream by improving the contaminated water quality without having a separate treatment tank upstream of the stream or the appeal is urgently needed.

The present invention has been proposed to solve the problems of the prior art as described above, the object of the present invention is simply to flow the algae breeding in rivers or lakes and various odors generated therefrom by simply flowing to the flowing or stored water. The present invention provides a method for removing green algae and odor in a river or lake using a removable microbial agent.

The technical problem of the present invention as described above is achieved by the following means.

(1) Bacillus amyl Laurier Quebec Pacifico Enschede (Bacillus amyloliquefaciens) to KS-4, Bacillus agar ladder lances (Bacillus agaradhaerens ) KS-5, Paenibacillus lentimorbus) KS-6, the novolak Bacillus La Ti kusu (Bacillus laevolacticus ) KS-7, Leuconostoc paramesenteroides ) KS-9, Kurthia sibirica ) KS-13 and sphingobacterium spiritiborum-GC subgroup B ( flavobacterium ) ( Sphingobacterium spiritivorum - GC subgroup B ( Flavobacterium ) ) A method for removing green algae and odors in a river or lake by introducing a complex microbial agent containing KS-18 into a river or lake.

(2) The method according to claim 1,

The complex microbial agent Bacillus sonorensis ( Bacillus sonorensis ) KS-33, Bacillus rickenformis ( Bacillus licheniformis ) KS-30, Bacillus circulans ) Green algae and odor removal method in a river or lake characterized in that it further comprises KS-80.

(3) Bacillus amyl Laurier Quebec Pacifico Enschede (Bacillus amyloliquefaciens) to KS-4, Bacillus agar ladder lances (Bacillus agaradhaerens ) KS-5, Paenibacillus lentimorbus ) KS-6, Bacillus laevolacticus KS-7, Leuconostoc paramesenteroides KS-9, Kurthia sibirica sibirica ) KS-13 and sphingobacterium spiritiborum-GC subgroup B ( flavobacterium ) ( Sphingobacterium spiritivorum - GC subgroup B ( Flavobacterium ) ) Algae and deodorants in rivers or lakes containing complex microbial agents comprising KS-18.

(4) The method according to claim 3,

The complex microbial agent Bacillus sonorensis ( Bacillus sonorensis ) KS-33, Bacillus rickenformis ( Bacillus licheniformis ) KS-30, Bacillus circulans ) Green algae and deodorants in rivers or lakes, characterized in that it further comprises KS-80.

According to the present invention, by simply flowing the complex microbial agent into the flowing or stored water, it is possible to easily and quickly remove the algae that grow in the river or the lake and various odors generated therefrom.

1 is a map showing a water collection point for measuring the water quality of the domestic A reservoir after the injection of the composite microbial agent according to the present invention.

Hereinafter, the contents of the present invention will be described in more detail.

The present invention, as described above, Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) KS-4, Bacillus agaradarens ( Bacillus agaradhaerens) KS-5, wave Enigma Bacillus alkylene Timothy booth (Paenibacillus lentimorbus) KS-6, the novolak Bacillus La Ti kusu (Bacillus laevolacticus ) KS-7, Leuconostoc paramesenteroides ) KS-9, Kurthia sibirica ) KS-13 and sphingobacterium spiritiborum-GC subgroup B ( flavobacterium ) ( Sphingobacterium spiritivorum - GC subgroup B ( Flavobacterium ) ) Provides a method for removing green algae and odors in a stream or lake by adding a complex microbial agent containing KS-18 to a stream or lake.

The microorganism used in the present invention is preferably Bacillus amyloliquefaciens ( Bacillus amyloliquefaciens ) KS-4 (KCTC 11186BP, available from Korea Institute of Biotechnology Gene Bank), Bacillus agaradaerens ( Bacillus agaradhaerens ) KS-5 (KCTC 11187BP, available from Korea Institute of Biotechnology Gene Bank), Paenibacillus lentibus lentimorbus) KS-6 (KCTC 11188BP , available pre-sale at the Korea Research Institute of Bioscience and Biotechnology Gene Bank), the Bacillus rockfish La Tea Syracuse (Bacillus laevolacticus ) KS-7 (KCTC 11189BP, available from Korea Institute of Biotechnology Gene Bank), Leuconostoc paramesenteroides ) KS-9 (KCTC 11190BP, available from Korea Biotechnology Research Institute Gene Bank), Kurthia Sibirica sibirica ) KS-13 (KCTC 11191BP, available from the Korea Institute of Biotechnology and Gene Bank) and Sphingobacterium Spiriborum - GC subgroup B ( Flambobacterium ) ( Sphingobacterium - spiritivorum - GC subgroup B ( Flavobacterium ) ) It is a complex microbial agent that contains KS-18 (KCTC 11192BP, available from Korea Biotechnology Research Institute Gene Bank).

Complex microbial agent according to the present invention does not require a special limitation, but each microbial strain may be mixed in a ratio of 1 to 30% by weight. The composite microbial agent according to the present invention may exert synergy by mixing the seven microorganisms to effectively remove green algae in rivers or lakes and the odor generated thereby.

In addition, the complex microbial agent according to the present invention Bacillus sonorensis ( Bacillus sonorensis ) KS-33 (KCTC 11354BP, Korea Institute of Bioscience and Biotechnology, deposited on June 25, 2008), Bacillus rickenformis ( Bacillus licheniformis) KS-30 (KCTC 11353BP , Korea Research Institute of Bioscience and Biotechnology Gene Bank, accession date 06/25/2008), Bacillus circulator Lance (Bacillus circulans ) KS-80 (KCTC 11355BP, Korea Research Institute of Bioscience and Biotechnology, deposited on June 25, 2008) is preferably included. When the composite microbial agent of the present invention further comprising the three microorganisms is introduced into the upstream or inlet of the stream or the lake, the sludge peeling rate is increased, and thus, the removal rate of the green alga is further improved. The three microbial strains may be added at a rate of 1 to 30% by weight, respectively.

Complex microbial preparations according to the present invention containing such microorganisms can be prepared by the following method.

0.001 to 0.02% by weight of the microorganism mixed species consisting of the above-mentioned microorganism strains (7 or 10 species), and the remaining water in 2 to 5% by weight of rice bran, 2 to 4% by molasses and 2 to 4% by weight of brown sugar After mixing to make 100% by weight to maintain a temperature of 20 to 25 ℃ repeatedly performed 2 to 6 times a day of aeration of air for 5 to 10 ㎥ / h for 1 to 3 hours at intervals of 2 to 6 hours And it can be cultured for 15 to 21 days to prepare a liquid fermentation agent. Conditions such as aeration is an optimized result of the study of the present inventors, and those skilled in the art will be able to add some modifications to the conditions, but it will be appreciated that such modifications do not depart from the scope of the present invention.

After preparing the liquid fermentation agent, a mixed material comprising 20 to 30% by weight of one or more culture materials selected from the group consisting of rice bran, agricultural by-products, and agricultural and forest products dry powder and 70 to 80% by weight of the liquid fermentation agent is prepared. Then, the inoculation step of inoculating 0.01 to 0.1% by weight of the microbial mixed seed according to the present invention can be performed.

The complex microbial agent of the present invention may be used as a cultivating material, rice bran, agricultural by-products or agro-forest products, which are relatively easy to obtain and have a low cost. The culture raw material may be used alone or in combination with the rice bran, agricultural by-products or agricultural products, preferably 35 to 45% by weight of rice bran, 25 to 35% by weight of agricultural by-products, 20 to 30% by weight of agricultural products It is preferable that it consists of the composition ratio of. The culture raw material is preferably ground to about 100 mesh to use in powder form. The mixed and pulverized culture raw materials are put into a direct incubator, and the water is adjusted with a liquid fermentation agent to maintain an environment suitable for microbial fermentation, followed by inoculation of the microbial mixed seed.

After performing the inoculation step, the inoculated mixed raw material is subjected to a high temperature culture step of incubating for 2 to 8 hours at 80 to 85 ℃. As described above, the method for producing a complex microbial agent of the present invention is characterized by culturing at an ultrahigh temperature of 80 ° C or higher. Considering that the general microbial culture is generally performed in the range of 20 to 40 ° C., it can be seen that the complex microbial preparation of the present invention is carried out under ultrahigh temperature conditions. In particular, in one embodiment of the present invention is cultured inoculated with the mixed seed is incubated at high temperature while stirring at a rate of 30 to 80rpm / min for 2 to 8 hours at 80 to 85 ℃. At this time, the culture temperature is carried out at 80 ℃ or more to suppress the growth of unnecessary microorganisms, it is preferable to perform at 85 ℃ or less to maintain the activity of the microorganism complex according to the present invention.

The method of injecting the complex microbial agent which can be prepared as described above is simply installed by installing a culture tank containing the complex microbial agent according to the present invention in a stream or an upstream portion of the lake and discharged through a guide device such as a hose. Can be processed. The complex microbial agent to be added depends on the degree of water pollution and the flow rate of the stream or the lake, but in general, 10 to 30 times the activation solution of the complex microbial stock solution is made 1 to 5 tons, preferably 2 to 4 tons daily The water is introduced several times at an interval of 1 to 3 days upstream of the stream or the lake.

The composite microbial stock solution is prepared by adding 0.1 to 1.0 parts by weight, preferably 0.2 to 0.7 parts by weight, and most preferably 0.5 parts by weight of molasses, based on 100 parts by weight of water, and 0.5 to 2.0 parts by weight of molasses. Preferably, 0.8 to 1.2 parts by weight, most preferably 1 part by weight of the culture was maintained while aeration for about 72 hours while maintaining at 18 to 25 ℃. For example, the activating solution may be prepared by adding 10 times water by weight to the composite microbial stock solution during the preparation of the 10-fold activating solution and containing 0.2 wt% molasses. Thereafter, the activation solution may be obtained by incubating with aeration for about 48 hours while maintaining the temperature at 18 to 25 ° C.

After completing the complex microbial preparation according to the present invention in the upstream or inlet of the stream or appeal, SS showed a tendency to fall continuously during the measurement period, and BOD and COD were measured the next day when the microorganism was completed. It is getting lower and lowering after peaking. In particular, COD, which is an important indicator of reservoir water quality, tends to be continuously lowered during the measurement period. The continuous reduction of COD means that microbial input can be a positive means of water quality improvement. In addition, T-N and T-P also peaked one week after the introduction of the combined microbial agent, or increased temporarily for a week and then gradually decreased.

When the composite microbial agent according to the present invention is introduced into a stream or an appeal, sludge containing green algae propagated in the sludge of the bed floats to the surface, and the odor is almost completely removed. In addition, the sludge is removed and the dark green of the riverbed returns to its original earthy color. According to an embodiment of the present invention, aquatic organisms sensitive to water quality, such as a snail breeding after one month after the incorporation of the complex microbial preparation according to the present invention, were found to be remarkably made.

Hereinafter, the present invention will be described in detail with reference to Examples and Test Examples. However, these are for the purpose of illustrating the present invention in more detail, and the scope of the present invention is not limited thereto.

≪ Example 1 >

Bacillus amyl Laurier Quebec Pacifico Enschede (Bacillus amyloliquefaciens ) KS-4 (KCTC 11186BP), Bacillus agaradaerens ( Bacillus agaradhaerens ) KS-5 (KCTC 11187BP), Paenibacillus lentimorbus ) KS-6 (KCTC 11188BP), Bacillus laevolacticus KS-7 (KCTC 11189BP), Leuconostoc paramesenteroides KS-9 (KCTC 11190BP), Curcia Sibirica ( Kurthia sibirica ) KS-13 (KCTC 11191BP) and Sphingobacterium Spiriborum-GC subgroup B ( Flambobacterium ) ( Sphingobacterium -spiritivorum-GC subgroup B ( Flavobacterium ) ) KS-18 (KCTC 11192BP) The mixed spawn 0.01kg, rice bran 4kg, molasses 2kg and brown sugar 4kg mixed with water suitable for drinking water standards to the weight of 100kg and then maintained at 20 to 25 ℃ and air of 10㎥ / h at intervals of 4 hours The process of aeration for 2 hours was repeated four times a day and cultured for 21 days to prepare a liquid fermentation agent. The liquid fermentation agent was 4.3 x 10 ⁹ cfu / g total bacteria.

Separately, the pre-crushed 65kg rice bran and 35kg agricultural by-products were placed in an incubator with a mixing function and mixed for 30 minutes. 30 kg of the liquid fermentation agent was added to the mixed raw materials to adjust the water concentration to 70%, and the microbial mixed seed according to the present invention was inoculated at 0.01% of the total weight of the culture raw material. The inoculated culture raw material was subjected to ultra high temperature culture for 4 hours while stirring at an outdoor temperature of 300 ° C., an incubator internal temperature of 80 to 85 ° C., and 30 to 80 rpm / min to prepare a complex microbial preparation of the present invention.

1 ton of water was added to 5 kg of the complex microbial agent, and 10 kg of molasses was added thereto, followed by incubation at 25 ° C. for 72 hours with aeration to obtain a stock solution. 10 times of water was added to the stock solution by weight, and 0.2 wt% molasses was added thereto to obtain a 10 times active solution.

The culture vessel containing the 10-fold active solution was installed in the water inlet of the domestic A reservoir (settling water storage amount: 12,000 tons), and then injected into the influent by 5 tons per day four times over 6 days, and then shown in FIG. The water quality was measured by measuring the degree of water quality improvement, and the results are shown in Table 1 (inflow water quality measurement results) and Table 2 (island, outer water quality measurement results).

As can be seen from the above results, SS was continuously decreased during the measurement period after the completion of the input of the composite microbial preparation according to the present invention, and BOD and COD showed the highest point when measuring the next day when the microorganism was completed, and then gradually decreased. In particular, it can be seen that COD, which is used as an important indicator of reservoir water quality, is continuously lowered during the measurement period.

<Example 2>

The 10-fold active solution of the composite microbial preparation of Example 1 was accumulated in the organic and inorganic substances on the bottom of the green algae, resulting in a bad smell of domestic B river for 2 days was injected into the upstream of the river for 37 days.

3 days after the input of the composite microbial preparation according to the present invention, green algae breeding sludge floated, odors disappeared, the color of the black sludge layer of the river is discolored, the top of the discolored sludge layer is microbial decomposition It was confirmed that it floated like a shell by action.

After 37 days of input, a large number of terrestrial surface organisms, which were difficult to find, appeared in large numbers, and real earthworms were continuously appearing on the bottom of the river. Odor was still not occurred, and sludge injuries were sustained, and the area where the original color of the river appeared was markedly increased, and the water quality improvement effect was confirmed.

<Example 3>

In addition to the strain of Example 1, the same amount of Bacillus sonorensis KS-33 (KCTC 11354BP), Bacillus rickenformis ( Bacillus) licheniformis) KS-30 (KCTC 11353BP ), and Bacillus circulator lance (Bacillus circulans ) 10 times the active microbial compound containing KS-80 (KCTC 11355BP), organic and inorganic materials accumulated in the riverbed, causing green algae. It was put in secondary artificial wetland.

After injecting the complex microbial agent according to the present invention, the green algae that began to thrive on the roots of the plants and the ground of the wetland facilities gradually dropped, and the earthworms increased in the artificial wetland facilities. The turbidity of the water was significantly improved and the odor of the effluent from the wetland facility was removed.

At the bottom of the wetland, the turbidity of the upstream, which had a high initial turbidity, gradually improved. In particular, it was confirmed that sludge and green algae in the bottom floated and removed, and mycelia occurred mainly in the nutrient-rich part of the river in the early stage of microbial input.

Near the middle and lower streams, a large amount of suspended solids occurred in the stream which had almost no suspended solids before treatment, and the odor disappeared toward the downstream. Compared with the existing amount of actual earthworms, a large amount of actual earthworms occurred, and sludge peeling proceeded. Mycelia and earthworms occurred after the microbial spray treatment on the sludge deposits at the edges of the stream, and the hyphae occurred where the green algae color of the stream changed and a large amount of contaminants were injected.

C The results of the following questionnaire survey on 67 persons through the face-to-face contact of riverside passengers and the face-to-face contact of three-sided riverside shopping malls are shown in Table 3.

※ Question: How does the current smell of C rivers compared to the previous year?

As described above, although described with reference to a preferred embodiment of the present invention, those skilled in the art will be variously modified and modified within the scope of the present invention without departing from the spirit and scope of the invention described in the claims below. It will be appreciated that it can be changed.

Korea Biotechnology Research Institute KCTC11353 20080625 Korea Biotechnology Research Institute KCTC11354 20080625 Korea Biotechnology Research Institute KCTC11355 20080625

Claims (4)

Bacillus amyloliquefaciens KS-4, Bacillus agaradhaerens KS-5, Paenibacillus lentimorbus KS-6, Bacillus laevolactus ) KS-7, Leuconostoc paramesenteroides KS-9, Kurthia sibirica KS-13, Sphingobacterium spiritiborum -GC subgroup B (Flavobacterium) ( Sphingobacterium spiritivorum-GC subgroup B (Flavobacterium) ) KS-18, Bacillus sonorensis KS-33, Bacillus licheniformis KS-30, and Bacillus circulans KS- Green microorganisms and odor removal method in a stream or lake characterized in that the microbial composite agent consisting of 80 to the river or lake to remove the algae and odor. delete Bacillus amyloliquefaciens KS-4, Bacillus agaradhaerens KS-5, Paenibacillus lentimorbus KS-6, Bacillus laevolactus ) KS-7, Leuconostoc paramesenteroides KS-9, Kurthia sibirica KS-13, Sphingobacterium spiritiborum -GC subgroup B (Flavobacterium) ( Sphingobacterium spiritivorum-GC subgroup B (Flavobacterium) ) KS-18, Bacillus sonorensis KS-33, Bacillus licheniformis KS-30, and Bacillus circulans KS- Green algae and deodorants in rivers or lakes comprising a microbial complex comprising 80.

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