CN102060389A - Composite microecological preparation capable of degrading nitrites in water body rapidly - Google Patents
Composite microecological preparation capable of degrading nitrites in water body rapidly Download PDFInfo
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- CN102060389A CN102060389A CN 201010538816 CN201010538816A CN102060389A CN 102060389 A CN102060389 A CN 102060389A CN 201010538816 CN201010538816 CN 201010538816 CN 201010538816 A CN201010538816 A CN 201010538816A CN 102060389 A CN102060389 A CN 102060389A
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Abstract
The invention discloses a composite microecological preparation capable of degrading nitrites in a water body rapidly. The composite microecological preparation is prepared from denitrifying bacteria, the lactic acid bacteria and the bacillus amyloliquefaciens are matched according to the ratio of 1:1:1 by taking denitrifying bacteria, lactic acid bacteria and bacillus amyloliquefaciens as active ingredients. The composite microecological preparation is characterized in that in the water body polluted with the nitrites, the composite microecological preparation with certain concentration is put, and the nitrites in the water body can be degraded rapidly in 24 h, therefore, the environment of the water body is improved, and the hazard of the nitrites to creatures in the water body is reduced.
Description
Technical field
The present invention relates to a kind of compound micro-ecological preparation of the water nitrite of degrading fast, belong to biological bacteria preparation technical field and environmental microorganism Application Areas.
Background technology
Nitrite mainly refers to Sodium Nitrite and potassium nitrite, because it can be converted into methemoglobin with the oxyphorase in the animal red blood corpuscle, makes the ability of blood cell forfeiture transportation oxygen, causes the animal hypoxia death, is a kind of highly toxic substance.
In recent years since chemical fertilizer application, sewage irrigation in the environment, form garbage and dejection, industrial nitrogenous waste, fuel combustion discharging nitrogenous discarded etc. under field conditions (factors), after decomposing, the precipitation leaching forms nitrite, inflow stream, lake also permeate the ground, thereby cause the nitrite of underground water and surface water to pollute.According to since 1978 to the investigation in nearly 50 cities, find to have the underground water in 21 cities to be subjected to the pollution of nitrite, wherein with serious pollution have 10 cities, had a strong impact on the safety of human drinking-water.And the pollution of the nitrite in the surface water worsens the water body coenocorrelation, has reduced the quality and the output of aquaculture product.Therefore, nitrite no matter be to human own, still can both cause direct or indirect harm to animal, plant to the pollution of water body.How to remove the nitrite in the water body, reduce its influence, become a problem that presses for solution the human lives.
Comprehensive domestic and international research present situation, the treatment process that is used for the water nitrite has several big classes such as chemical method, biological process and physics method.And chemical method and physics method are subjected to various treatment facilities in specific implementation process, economic condition, and the restriction of multiple factors such as later stage waste discharge, and biological method is with low cost, easy to use owing to it, multiple advantage such as no waste is more and more used.
Milk-acid bacteria is more and more used as the listed feed interpolation microbial inoculum of the Ministry of Agriculture, and the effect of its degraded water nitrite also has report more.Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) HN, identify through 16S rDNA order-checking, be preserved in China Committee for Culture Collection of Microorganisms common micro-organisms center, preserving number CGMCC No.3261, nontoxic after measured, have the effect of degraded water body Central Asia nitrate.Denitrifying bacterium is reduced to the nitrate in the water body, nitrite nitrogen and discharges into the atmosphere by the aerobic denitrification, thereby reaches the purpose of denitrogenation.The present invention is combined into efficient probiotics with three kinds of denitrification functions bacterial strains, the water body Central Asia nitrate of can degrading fast, and nitrate reaches the purpose that purifies water.
Summary of the invention:
The present invention is from multiple water surroundings such as pond and blowdown irrigation canals and ditches, separate can the efficient degradation water nitrite three class bacterial strains, and test by purification of water quality, filter out the best complex ratio of three kinds of bacterial strains, be developed into efficient compound micro-ecological preparation, nitrite and nitrate in the water body of can efficiently degrading fast.
The compound micro-ecological preparation of degraded water body Central Asia nitrate is by denitrifying bacterium among the present invention, milk-acid bacteria, and three kinds of bacterial strains of bacillus amyloliquefaciens are formed.Denitrifying bacterium wherein is that false unit cell belongs to a kind of of (Pseudomonassp.), and milk-acid bacteria is that streptococcus acidi lactici belongs to a kind of of (Streptococcus sp.), bacillus amyloliquefaciens preserving number CGMCC No.3261.
The bacterial concentration of three kinds of bacterium of the present invention is respectively and all reaches 3*10
9More than the cfu/mL.
Compound micro-ecological preparation among the present invention is that 1: 1: 1 proportioning is made by the fermented liquid of denitrifying bacterium, milk-acid bacteria and three kinds of bacterium of bacillus amyloliquefaciens by volume.
Indoor purification of water quality test shows that 29 ℃ of water temperatures, nitrite concentration is 12mgL in the simulated wastewater
-1The time, this compound micro-ecological preparation can make nitrite degradation more than 99% within the 24h, and significantly reduces the content (degradation rate reaches 99%) of nitrate simultaneously, reduces the pH value of water body, and it is constant relatively that ammonia nitrogen keeps, and decontamination effect improving significantly is better than contrast.25 ℃ of water temperatures, nitrite concentration is 13mgL in the simulated wastewater
-1The time, this compound micro-ecological preparation reaches 81.8% to the nitrite degradation rate within 24h, within 48h the nitrite degradation rate is reached 97.0%.22 ℃ of water temperatures, nitrite concentration is 12mgL in the simulated wastewater
-1The time, this compound micro-ecological preparation reaches 75.3% to the nitrite degradation rate within 48h.
The safety testing of microbial inoculum proves that this microbial inoculum is nontoxic to the growth of fry.Can with produce in go.
Embodiment:
The water quality purification function screening of embodiment 1 single strain
Denitrifying bacterium is to the degradation effect of nitrite
With dress 2.5L analog culture waste water in the Glass Containers, the starting point concentration of adjusting nitrite nitrogen is 35mgL
-1, place in 30 ℃ of incubators behind the access 50mL bacterium liquid and cultivate, be contrast not add bacterium, timing sampling is surveyed the content of nitrite in the water.Result such as Fig. 1.
As seen from Figure 1, insert denitrifying bacterium in water body, 24h is interior to be 32mgL to nitrite concentration
-1The degradation rate of simulated wastewater is 60.9%, within 40h nitrite is degraded fully, and degradation effect significantly is better than contrast.
Milk-acid bacteria is to the degradation effect of nitrite
With dress 3L analog culture waste water in the Glass Containers, the starting point concentration of adjusting nitrite nitrogen is 12mgL
-1, insert the bacterium mud behind the 30mL bacterium liquid centrifugal (the centrifugal 10min of 7500rpm), be contrast not add bacterium, cultivate the content of nitrite in the timing sampling survey every day water in placing 25 ℃.Result such as Fig. 2.
As seen from Figure 2, insert milk-acid bacteria in water body, 24h is interior to be 12mgL to nitrite concentration
-1The degradation rate of simulated wastewater is 76.9%, within 48h nitrite is degraded fully, and degradation effect is better.
Bacillus amyloliquefaciens is to the degradation effect of nitrite
With dress 2.5L analog culture waste water in the Glass Containers, the starting point concentration of adjusting nitrite nitrogen is 13mgL
-1, the bacterium mud behind the access 40ml bacterium liquid centrifugal (the centrifugal 10min of 7500rpm) is contrast not add bacterium, is placed in 30 ℃ of incubators and cultivates, every day, timing sampling was surveyed the content of nitrite in the water.Result such as Fig. 3.
As seen from Figure 3, insert bacillus amyloliquefaciens in water body, 24h is interior to be 12mgL to nitrite concentration
-1The degradation rate of simulated wastewater is 19.2%, and the nitrite degradation rate is 94.8% within 120h, and degradation speed is slower, but significantly is better than contrast.
The effects of purification quality of embodiment 2 compound micro-ecological preparations (29 ℃)
Configuration analog culture water: culturing pool mixes in indoor placement 2 days and tap water after the sand filter at 1: 1, measures initial nitrate, nitrite content and pH value in the mixing water.Add an amount of nitrite, making the nitrite nitrogen in the water is 12mgL
-1Each mug 3L water.
Connect the bacterium method: will remove supernatant behind the bacillus amyloliquefaciens 10mL and lactic acid bacterial liquid 10mL centrifugal (the centrifugal 10min of 7500rpm) respectively, bacterium mud is added water body, denitrifying bacterium directly adds 10ml.Not add bacterium is contrast, and each handles 3 repetitions.
Increase illumination 7-8h with incandescent light daytime, and water temperature is about 29 ℃.Each water sampling after 7500rpm is centrifugal, is got supernatant liquor and is measured.
As seen from Figure 4, connect bacterium under 29 ℃ and handle and compare with contrast, the nitrite degradation within 24h in the water body is comparatively complete, and degradation rate reaches 99.7%.As seen from Figure 5, within 24h, connect the degradation rate that bacterium handles nitrate and reach 97.8%.Degradation effect significantly is better than contrast.As seen from Figure 6, connect the bacterium processing and compare the pH value that can significantly reduce water body with contrast.
The effects of purification quality of embodiment 3 compound micro-ecological preparations (25 ℃)
Test method is with embodiment 2.Water temperature is about 25 ℃.
As seen from Figure 7, connect bacterium under 25 ℃ and handle and compare with contrast, the degradation rate to nitrite within 24h reaches 81.8%, and the degradation rate to nitrite within 48h reaches 99.9%.As seen from Figure 8, connect the degradation rate that bacterium handles nitrate and reach 82.7% within 24 hours, the degradation rate to nitrate within 48h reaches 97.0%.Degradation effect is lower than the degradation effect under 29 ℃.As seen from Figure 9, connect the bacterium processing and compare the pH value that can significantly reduce water body with contrast.
The effects of purification quality of embodiment 4 compound micro-ecological preparations (22 ℃)
Test method is with embodiment 2.Water temperature is about 22 ℃.
As seen from Figure 10, connect bacterium under 22 ℃ and handle and compare with contrast, the degradation rate to nitrite within 24h reaches 31.3%, and the degradation rate to nitrite within 48h reaches 76.0%, and nitrite content kept stable, no longer degraded later on.As seen from Figure 11, connect the degradation rate that bacterium handles nitrate and reach 27.0% within 24h, the degradation rate to nitrate within 48h reaches 75.3%, and nitrate content kept stable, no longer degraded later on.Degradation effect be lower than under 29 ℃ with 25 ℃ of degradation effects.Illustrate that temperature has considerable influence to the effect of composite bacteria degraded water body Central Asia nitrate.As seen from Figure 12, connect the bacterium processing and compare the pH value that can significantly reduce water body with contrast.
Claims (4)
1. the compound micro-ecological preparation of the water body Central Asia nitrate that is used to degrade is characterized in that: with denitrifying bacterium, and milk-acid bacteria, bacillus amyloliquefaciens is an activeconstituents, is composited by the fermented liquids of above-mentioned three kinds of bacteriums.
2. the compound micro-ecological preparation of the water body Central Asia nitrate that is used to degrade according to claim 1, it is characterized in that: the denitrifying bacterium in the preparation is that false unit cell belongs to a kind of of (Pseudomonas sp.), milk-acid bacteria is that streptococcus acidi lactici belongs to a kind of of (Streptococcus sp.), bacillus amyloliquefaciens preserving number CGMCC No.3261.
3. according to the compound micro-ecological preparation of the arbitrary described water body Central Asia nitrate that is used to degrade of claim 1 to 2, it is characterized in that: the fermented liquid of denitrifying bacterium, milk-acid bacteria and three kinds of bacterium of bacillus amyloliquefaciens is 1: 1: 1 by volume; The fermented liquid concentration of three kinds of bacterium is all more than 3*109cfu/mL.
4. according to claim 3 multiplexing in the compound micro-ecological preparation of degraded water body Central Asia nitrate, it is characterized in that: the nitrite in the water body of degrading fast, when 29 ℃ of water temperatures, this compound micro-ecological preparation within 24h to containing 12mgL
-1Nitrite degradation rate in the simulated wastewater of nitrite reaches 99.7%.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103667122A (en) * | 2013-11-27 | 2014-03-26 | 北京龙科方舟生物工程技术有限公司 | Compound bacterium and application thereof |
| CN104556403A (en) * | 2014-12-29 | 2015-04-29 | 天津振邦水产养殖有限公司 | Method for reducing eutrophication in sea cucumber growth waters |
| CN105543139A (en) * | 2016-01-12 | 2016-05-04 | 江南大学 | Bacillus amyloliquefaciens for effectively degrading nitrite |
| CN111348753A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Method for enhancing denitrification of denitrifying microorganisms |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1928072A (en) * | 2006-06-05 | 2007-03-14 | 宗绪成 | Microorganism bacterium agent for treating septic tank and city planting ductwork sewage and sludge and production method |
| CN101717724A (en) * | 2009-12-17 | 2010-06-02 | 天津市农业生物技术研究中心 | Compound microecological preparation used for water body purification of aquaculture |
-
2010
- 2010-11-10 CN CN 201010538816 patent/CN102060389A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1928072A (en) * | 2006-06-05 | 2007-03-14 | 宗绪成 | Microorganism bacterium agent for treating septic tank and city planting ductwork sewage and sludge and production method |
| CN101717724A (en) * | 2009-12-17 | 2010-06-02 | 天津市农业生物技术研究中心 | Compound microecological preparation used for water body purification of aquaculture |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102719383A (en) * | 2012-06-29 | 2012-10-10 | 武汉工业学院 | Bacillus amyloliquefaciens, inoculant and application thereof |
| CN103667122A (en) * | 2013-11-27 | 2014-03-26 | 北京龙科方舟生物工程技术有限公司 | Compound bacterium and application thereof |
| CN103667122B (en) * | 2013-11-27 | 2015-06-10 | 北京龙科方舟生物工程技术有限公司 | Compound bacterium and application thereof |
| CN104556403A (en) * | 2014-12-29 | 2015-04-29 | 天津振邦水产养殖有限公司 | Method for reducing eutrophication in sea cucumber growth waters |
| CN105543139A (en) * | 2016-01-12 | 2016-05-04 | 江南大学 | Bacillus amyloliquefaciens for effectively degrading nitrite |
| CN111348753A (en) * | 2018-12-21 | 2020-06-30 | 中国石油化工股份有限公司 | Method for enhancing denitrification of denitrifying microorganisms |
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Application publication date: 20110518 |