CN101676225B

CN101676225B - A new microbe agent and preparation method thereof

Info

Publication number: CN101676225B
Application number: CN200810211985A
Authority: CN
Inventors: 范德朋
Original assignee: 佛山市碧沃丰生物科技有限公司
Priority date: 2008-09-16
Filing date: 2008-09-16
Publication date: 2012-10-17
Also published as: CN101676225A

Abstract

A new microbe agent comprises enzyme and microbe, nutrient and anticrustator, wherein the enzyme comprises protease, amylase, cellulose, lipase, with weight percentage of 0.025-5%; the microbe comprises: lactococcus lactis, lactobacillus plantarum, bacillus subtilis, beer yeast or saccharomyces cervisiae, streptococcus faecium, with weight percentage of 0.125-25%; the nutrient comprises: sucrose, whey, molasses, carrier without wheat bran and rice bran, with weight percentage of 68-95%; the weight percentage of sodium alumino-silicate as the anticrustator is 0.5-3%.The preparation method comprises: culturing the inoculant in a bio-fermentor for at least 12 hours at temperature of 7.2-65.5 DEG and detecting; detecting, supplying sterile sucrose and oxygen at pH value of 5.5-9.5 and detecting; obtaining concentrated bacteria in 24 hours and loading the dextran capsule; quickly freezing at -4.5-0 DEG and freeze-drying for 2-4 days and mixing the other components with the concentrated bacteria and packaging the mixture into finished products.

Description

A kind of new microbe agent and preparation method thereof

Technical field

The present invention relates to a kind of microbiobacterial agent and preparation method thereof, particularly a kind of new microbe agent and preparation method thereof.

Background technology

Biotechnology has been to use the widest, of paramount importance single technology in the environment protection, and all respects of the environment protection such as reparation of its degraded in water pollution control, hazardous and noxious substances, changing waste into resources, contaminate environment are being brought into play very important effect.

When the applied environment biotechnology was handled pollutent, final product all was nontoxic, stable material, like carbonic acid gas, water and nitrogen.

Utilizing biological method to handle pollutent can settle at one go usually; Avoided the repeatedly transfer of pollutent, so it is a kind of decontamination safety and method, the particularly develop rapidly of modern biotechnology such as cell engineering and enzyme engineering and application completely; Strengthened the environmental organism treating processes greatly; Make biological treatment have higher efficient, lower cost and better specificity are for more wide prospect has been showed in the application of biotechnology in environment protection.

EPA (EPA) also points out " distinguishing feature that the biological treating technology is superior to other new technologies is that it is pollutent technology for eliminating rather than separated from contaminants technology " when estimating Environmental Biotechnology.

Along with increasing of world population, The development in society and economy, the improving constantly of people's level of consumption; Each pollutant accumulates fast, and the physical environment that the mankind depend on for existence suffers more and more serious destruction, and the survival and development pressure that the mankind face is increasing; And traditional chemical physics processing mode can't thoroughly solve problem of environmental pollution, and therefore the compound prescription of " mikrobe+enzyme " arises at the historic moment; This nature of following utilizes the mikrobe of nature existence itself and the method for enzyme, can not break nature original ecological balance and material element circulation law; Adhere to the theory of biological prosthetic, thereby accomplish thorough degraded and reduction pollutent.

Aspect aquaculture; Originally in order to control water pollution and harmful germ; What adopt is the regular disinfectant method of pharmaceutical chemicals, and water quality can't be really controlled on the one hand, often worsens repeatedly; The a large amount of mud of pond bottom sediments, the drug residue problem has seriously reduced the quality and the price of fishery products on the other hand.And adopt the enzyme compound prescription, and then can the residual bait in the water body, ight soil thoroughly be degraded into carbonic acid gas, nitrogen and water, mud at the bottom of the minimizing pond, it is stable that water quality keeps, and cultures the product quality and be greatly improved.

Aspect WWT, original sewage work unloading phase of system, can only arrive other Sewage Plant fortune mud as provenance, and the effect of promptly requiring great effort is bad again, need 2-3 month start time, and success ratio is low.Add the enzyme prescription and adopt, do not need to transport again mud, can reduce the manpower and materials input, can guarantee in week to start successfully at 3-4.In sewage work's operational process, use flocculation agent in a large number, behind flocculation sediment, produce a large amount of excess sludges, these mud can't be disposed, and can only arrive the field in the past and bury, and cause secondary pollution.And add the enzyme prescription since the bacterium in the prescription to organism decompose more thorough, then can reduce the excess sludge generation.Simultaneously, with excess sludge with after animal excrement mix, through adding enzyme compost series products; Ferment, make it become fertilizer, price per ton can reach 600-800 unit; Both solved excess sludge waste residue problem; Created new economic worth again,, realized the sustainable development path of recycling economy the refuse back to nature.

Therefore, the microbiobacterial agent compound prescription of " mikrobe+enzyme " is the new technology that promotes the well-being of mankind that is worthy to be popularized very much.

Summary of the invention

The object of the present invention is to provide a kind of novel utilize biological prosthetic to the thorough degraded of pollutent and reduction, applied widely, result of use stable, microbiobacterial agent of long service life, safe and convenient to use, environmental protection and preparation method thereof.

The objective of the invention is to realize through following technical scheme:

A kind of new microbe agent comprises enzyme and bacterium, nutrition and good antiscale property agent:

(1) said enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 0.025～5%;

(2) said bacterium comprises lactococcus lactis ssp, plant lactobacillus, subtilis, cereuisiae fermentum or yeast saccharomyces cerevisiae, streptococcus faecalis, and its composition weight percent is: 0.125～25%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 68～95%;

(4) weight percent of said good antiscale property agent sodium aluminum silicate is: 0.5～3%.

Said lactococcus lactis ssp is a streptococcus acidi lactici.

Said streptococcus faecalis is a faecium.

A kind of method for preparing above-mentioned new microbe agent the steps include:

(1) bacterium through screening grows in substratum, and after detecting, the inoculum of the first-generation is transferred to the growth of in the biological fermentation device of aseptically process, carrying out at least ten two hours, and said bacterial growth temperature is: 7.2 ℃～65.5 ℃;

(2) after purity is confirmed in quality examination; Inoculum is transferred in the stainless steel fermentation container of sealed, sterile, and aseptic sugar and oxygen supply are given bacterium and controlled suitable pH value, said pH value: average range is 5.5～9.5; In the whole cycle, sampling check does not regularly have assorted bacterium situation and growth indexes;

In (3) 24 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium, micro capsule technology installs spissated bacterium additional VISOSE outer capsule, and this technology both makes it when storing and mixing with vehicle that protection against the tide also maintains vigour;

(4) subsequently product in the fluid refrigeration system by quick freezing; And at large-scale refrigeration chamber; Temperature is under-4.5～0 ℃ of condition, carries out 2～4 days lyophilize, and twice freezing dry process eliminated 95% moisture; Guaranteed the high viability of bacterial classification, mixing behind other system component is packaging final prod.

Said dissolved oxygen amount >=2 mg/litre.

Said trace element: nitrogen >=5ppm.

Said trace element: phosphorus >=1ppm.

Said salinity≤40 ‰.

The present invention can reach following beneficial effect after adopting above technical scheme

1, initiative.The present invention is " bacterium+enzyme ", rather than independent " bacterium " or " enzyme ".

Biologics before or be " bacterium prescription ", or be " the diseaseful prescription of enzyme " and " enzyme prescription "; Yet microbiology shows, the help digest specific enzyme of food of all natural generation of all bacteriums, and enzyme becomes the convenient nutritive substance that absorbs for a short time and supplies with bacterium and use through big mixture (pollutent) is changed.The product of " bacterium prescription " or " the diseaseful prescription of enzyme " relies on bacterium generation enzyme itself to carry out work, and regrettably these products are unstable, and the bacterial strain that relies on effectiveness to weaken greatly obviously can't be accomplished mission; Only use the product of " enzyme " can only food source be converted into simpler form but not complete digestion is so just shirked next level and caused more serious secondary pollution problem.The present invention makes it suitable bacterial strain and the preparation of enzyme bonded can bring into play effect immediately, and digestion at once can make bacterial strain be attached to tightly on the source of pollution until till eating to the greatest extent.

In view of the consideration of theoretical and actual two aspects, spontaneous growth, the mikrobe of no genetic modification is added in the product,, forms through microcapsule packaging process and the configuration of unique enzyme treatment technique from the bacterium of occurring in nature screening by fully.

At first, select mikrobe and in the laboratory, isolated and check, analyze it separately to the degradation capability of chemical structure.

The further interaction of test bacterium family under varying environment.

Identify and in entire test, all show good bacterium family, and their required energy derives.

In numerous tests, draw feasible biology and expand mixture, in various systems, as one man remove target contaminant, and economical, efficient.

2, applied widely.Product among the present invention can be widely used in industries such as aquaculture and the biological compost in municipal administration and industrial sewage processing, landscape water improvement, petroleum-contaminated soil reparation, the ecological agriculture, the following characteristics of product tool: its stability, ageing unprecedented in the same industry; Safe and convenient to use, nontoxic, there is not corrosion, harmless to the people and animals plant-animal, tool biological degradability, ecological security, no negative impact.

3, stable performance.Product among the present invention is the bacterium of screening from occurring in nature fully, forms through microcapsule packaging process and the configuration of unique enzyme treatment technique.The microcapsule packaging process makes bacterium be in " dormancy " state, can make shelf life of products at least more than 2 years, when product with after water mixes, its resist is dissolved, can guarantee that the first-generation bacterium of maximum dose level is devoted oneself to work immediately.

4, reasonableness.Product among the present invention is " bacterium+enzyme " rather than single bacterium or enzyme.

Enzyme becomes the nutritive substance of bacterium rapid absorption through big pollutent is cracked into small molecules, makes it bring into play effect at once, and digestion at once can make bacterial strain be attached on the source of pollution till degraded fully.

The effectiveness of enzyme-unique enzyme treatment process

Enzyme is the protein that is produced naturally and depended on for existence by plant, animal, bacterium, fungi and all life entities, and it is to promote chemical reaction and the catalyzer that do not change self.The enzyme of commercial use results from bacterium and fungi, as: Bacillaceae, genus aspergillus and trichoderma etc.

Enzyme changes into the micromolecular convenient nutritive substance supply bacterium that absorbs with macromolecular mixture (substrate) and uses, and the enzyme in source reaches best effectiveness together, requires specific temperature and pH; This needs emphasis to consider for selecting enzyme product, can classify by the substrate of its effect, for example; Proteolytic enzyme acts on protein, and protein is resolved into amino acid and peptide, and the cellulase decomposition of cellulose becomes simpler sugar; And Mierocrystalline cellulose is the composition that plant cell wall can not be digested, and a spot of enzyme just can a large amount of substrate of catalysis, and typical enzyme-to-substrate ratio is 1:1; 000 to 1:1, and 000,000.

In order to keep rendeing a service, test that enzyme require stands to store and opposing acidifying, hydrolytic action.Enzyme among the present invention uses preferred microorganism bacterial strain and best-of-breed technology to keep its stability, and after mixing, the effect of enzyme does not receive long-time storage, oxide compound (like VITAMINs and mineral substance) and digestive environmental influence.

5, environmental protection.Microbiobacterial agent ecological security among the present invention, nontoxic, there is not corrosion, the tool biological degradability, no negative impact, harmless to people, plant-animal.

6, investment is little.Production fixed investment of the present invention is few, and cost is low, and efficient is high, and speed is fast, and is easy and simple to handle, do not destroy the working-yard physical environment.

7, pure biological.The present invention thoroughly takes leave of or reduces costliness and the use of the big pharmaceutical chemicals of negative impact, and product is innoxious substances such as carbonic acid gas, water, nitrogen, does not produce secondary pollution.

8, effective.Flexibility of the present invention is strong, and its high-strength performance makes it Working environment and reaches bigger pH, temperature, salinity range.

9, cut down the consumption of energy.Reaction conditions of the present invention is gentle, does not need HTHP, reduces energy consumption.

Embodiment

Below in conjunction with specific embodiment the present invention is done further explain:

Embodiment 1

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 0.125～25%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 0.5～3%.

The preparation method of said new microbe agent, step is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 0.125～25%; In substratum, grow; After detecting; The inoculum of the first-generation is transferred in 250 liters of biological fermentation devices of aseptically process and carries out at least ten two hours growth, and said bacterial growth temperature is: 7.2 ℃～65.5 ℃.

(2) after purity was confirmed in quality examination, inoculum was transferred in 5000 liters of stainless steel fermentation containers of sealed, sterile.Aseptic sugar and oxygen supply are given bacterium and are controlled suitable pH.In the whole cycle, sampling check does not regularly have assorted bacterium situation and growth indexes.

In (3) 24 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium.Micro capsule technology installs spissated bacterium additional VISOSE outer capsule.This technology both makes it when storing and mixing with vehicle, and protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-4.5～0 ℃ of condition; Carry out 2～4 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 0.025～5%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 68～95%; The weight percent of good antiscale property agent sodium aluminum silicate (NaAlSi3O8) is: 0.5～3%, be finished product.

(5) every batch of product all passes through no Salmonellas detection.

Said bacterial classification: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also is claimed faecium (Enterococcus faecium); All buying is from " Guangdong Microbes Inst ", and other entity and individual all can obtain above-mentioned bacterial classification through buying to this unit at any time.

Above-mentioned bacterial classification is extensively to exist at nature, and the engineered product of non-genomic is listed in " allow use fodder additives kind catalogue " by China Ministry of Agriculture, explain that above bacterial classification is nontoxic, and is harmless to people and animals, plant-animal, the security of tool environmental ecology.

The validity that microbiobacterial agent is applied to the biological prosthetic reinforcement schedule depends on the environment of place to use at that time; These envrionment conditionss have determined the efficient and the corresponding using dosage of degraded, and the biodegradable time also depends on emulsification unit, envrionment conditions, blend level and other factors simultaneously.Through test shows, following physics and chemical parameters are grown up the most effective to bacterium:

1, pH value: average range is between 5.5～9.5.

2, temperature: can between 7.2 ℃～65.5 ℃, come into force.Temperature is higher than 65.5 ℃, can cause the death of bacterium; Bacterium can be not dead when temperature was lower than 7.2 ℃, but its cell growth can receive very big restriction.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount reach 2 mg/litre at least; The bacterium family of contained high adaptive faculty will accelerate 5～7 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

4, trace element: the meeting in its growth of proprietary bacterium family needs many elements, like potassium, iron, calcium, sulphur, magnesium etc., all can contain the element of above capacity usually in soil and the water source.

5, nitrogen: need 5ppm at least.

6, phosphorus: need 1ppm at least.

7, salinity: in seawater and fresh water, all be suitable for the highest 40 ‰ the salinity that tolerates.

8, toxin immunity: can resist the chemical toxicity material more effectively, comprise muriate, prussiate and heavy metal etc.

9, note: when sterilant is contained in contaminated district, should study them in advance action of microorganisms.

Product efficacy

1, degraded COD, BOD, TSS, ammonia nitrogen, nitrite, phosphorus etc.

2, decompose mud, organic compositions such as specific degraded bloodstain, grease, scum silica frost, alluvial.

3, odorous gas such as removal of hydrogen sulfide, ammonia nitrogen and foam, scum silica frost.

4, organic dirts such as degraded ight soil, kitchen thing, rubbish.

5, degraded oil hydrocarbon polymer.

6, degraded polycyclic aromatic hydrocarbons.

The biological prosthetic intensifying technology of Waste Water Treatment

Select " bacterium+enzyme " product for use, the remediation intensification Sewage treatment systems improves system efficiency and stability, makes indexs such as COD, ammonia nitrogen reach state emission standard.

With biochemical section is main remediation intensification unit, increases anaerobism (acidic hydrolysis) unit and renders a service, and COD is changed to BOD, increases biodegradability, renders a service to promote for aerobic unit and carries out pre-treatment.Fast culture is tamed aerobic flora in the aeration tank, start-up system, rapid biofilm success; Increase dominant microflora quantity on the unit surface, improve volumetric loading, increase the residence time relatively; Reach lifting aerobic unit efficient, significantly strengthen the purpose of integrated artistic level of performance, can in a heavy pond and second pond, add Black Liquor with Efficient Bacteria in case of necessity; Increase active sludge subsequent disposal effectiveness in a heavy pond acidic hydrolysis and the second pond, it is escalated into the unit with biochemical function, prolong the effectively biochemical time.

(1) adopt biological reinforced mode, the design and construction treatment project than the conventional design scheme, can reduce by 20～30% fixed investments.

(2) adopt the old system of biological prosthetic, need not increase new investment in fixed assets, improve system efficacy in combination 30～50%, can let system's water outlet up to standard, reduce at least 20% than other mode costs.

(3) start recovery system fast, can shorten to 15～20 days cultivating the domestication time.Use manpower and material resources sparingly 10～20%.

(4) system's long-time running adds microbial preparation, can improve system stability and impact resistance, reduces the use of pharmaceutical chemicalss such as flocculation agent, reduces the electric power energy consumption, reduces running cost 20～30%, and the microbial preparation medicament expense only increases by 0.2～0.5 yuan of/ton water.

(5) surplus sludge volume is 1/2 of a common process, reduces the sludge disposal expense.

(6) can special some specific pollutant of degraded, solve a waste water difficult problem not up to standard.

The ecological reestablishment of rivers and lakes landscape water body

Through adding external source bacterial classification and enzyme, organic pollutant and remove nitrogen, phosphorus in the digestion degradation water improves water body species diversity and self-purification capacity, solves the pollution problem of algae and bed mud.

Through artificial-strengthening measures such as water body oxygenation, bacterizations, impel the single type ecosystem to compound ecosystem succession, thereby improve water body species diversity and self-purification capacity.According to the raw water quality water yield, water body is carried out preoxygenation, activated bacterium and enzyme get in the water body then, when carrying out abundant oxygenation, detect each contamination index and change, and for raising the efficiency the mode of taking the secondary sowing, strengthen the improvement phase after 7 to 10 days.Get into to strengthen and administer after date, according to Analysis Results of Water Quality, the prescription kind of adjustment mikrobe and enzyme is carried out the specific aim reparation, and each item water-quality guideline is progressively reached governance standard, and the reinforcement improvement phase needs 30～60 day time.

(1) fixation means less investment, the construction time is short, and noise is few, does not destroy ecotope on every side.

(2) the similar sewage work of result compares with similar conventional art, and construction investment and working cost can save at least 30%.

(3) processing cost is low, administers to take 0.5～1.5 yuan of/ton water, and the long-time maintenance cost is 0.1～0.3 yuan of/ton water year only.

(4) through intensified process, go the speed of mud to be about 0.1 meter in every month, fundamentally administer and pollute, reduce the desilting disposal costs.

(5) instant effect is treated both principal and secondary aspect of disease, and need not add any chemical agent, does not produce secondary pollution, the graceful ecotope of real establishment.

Culture fishery ecologic breeding new model is promoted

In breeding environment, add external source viable bacteria and enzyme; With refuse effective conversion in the water body is innoxious substance, through competitive inhibition, reduces the quantity of vibrios, blue-green algae and photogenic bacterium etc.; Regulate and culture balance of ecosystem in the pond, the purpose of reach cultivated animals health, growing fast.

Select 8 identical breed ponds of condition to experimentize, establish A, B, C, D altogether, 4 groups, establish 2 for every group and culture the pond as parallel-group, wherein the A group is control group, B, C, D are experimental group, to B, C, D component Bie Shijiaben product and other two kinds of microbiobacterial agents.Each pond daily administration of experimental session all is consistent, each culture the pond every at a distance from 10 days in 8:00 fixed point (3 points are got in every pond) water sampling in the morning, mensuration ammonia nitrogen, chemical oxygen demand, basicity and pH.All data are the MV in each 2 pond of experimental group.After the results, experiment finishes, and the data such as survival rate, unit per mu yield, pure profit and ratio for input and output of each experimental group are analyzed, and sums up.

(1) than the conventional cultivation pattern, improve weed survival rate 20～30%, improve the seed speed of growth 10～15% relatively.

(2) more common aquaculture model, indexs such as water quality ammonia nitrogen, COD, basicity on average descend 30%, 10%, 25%.

(3) than the conventional cultivation pattern, per mu yield, pure profit and ratio for input and output improve 30～40%, 80～90% and 50～60% respectively.

(4) an ecologic breeding moisturizing, (every day, moisturizing 2～5cm), and tradition is cultured every mu and saved 300～500 yuan of the electricity charge relatively not change water.

(5) in the ecologic breeding process, the cultivated animals desire of ingesting is vigorous, and digestibility is high and stablize, reduces feed coefficient 0.1～0.3,200～300 yuan of every mu of saving feed costs.

(6) no longer timing is to water body disinfection in the ecologic breeding process, and zeolite powder (ground dolomite), lime powder etc. are used in minimizing, saves 100～150 yuan of costs for every mu.

(7) ecologic breeding is in time cleared up bottom sludge, reduces the desilting work of culturing in time season.

(8) finished product of ecologic breeding, body colour is bright, brawniness is full, delicious flavour, the common relatively article of price are high.

The improvement technology of crude oil and verivate contaminated soil thereof

Add degrade specifically function yeast and enzyme in contaminated soil, pollutent is degraded into carbonic acid gas and water fast or changes into harmless material, make its natural function of soil restoration.

Confirm soil structure type, soil is carried out scarifying.Detect pH, adjust to 6.6～7.4.Spray water soluble composite fertilizer, adjust to 100:5:1 to major general C:N:P.After the product dissolving, 1/2 solution is evenly splashed on soil.Stir soil, the residue of evenly splashing 1/2 solution.Water spray, it is best regulating soil humidity to 25～50%, 25～30%.Cover (use black plastic film when thickness 0.15～0.51mm), temperature are low with the white plastic film.Detect soil humidity every day, remain on 25～30%.Stir soil weekly, detect pH, detect C:N:P, adjust if necessary.After 2 weeks, 4 weeks, detect oleaginousness and change.

(1) original position reparation, on-the-spot disposal has been avoided the secondary pollution in the defeated process of collection, has practiced thrift processing costs.

(2) than physico-chemical processes costs such as traditional excavation, burning, washing low 30%.

(3) remaining residual contamination thing can be reduced to extremely low-levelly, does not produce secondary pollution, does not destroy the required edatope of plant-growth.

(4) remote districts can be repaired, the place of legacy equipment can not be used.

Embodiment 2

(1) said enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 0.5%;

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 14%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 85%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 0.5%.

The preparation method of said new microbe agent, step is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 14%; In substratum, grow; After detecting; The inoculum of the first-generation is transferred in 250 liters of biological fermentation devices of aseptically process and carries out 12 hours growth, and said bacterial growth temperature is: 10.5 ℃.

(2) after purity was confirmed in quality examination, inoculum was transferred in 5000 liters of stainless steel fermentation containers of sealed, sterile, and aseptic sugar and oxygen supply are given bacterium and controlled suitable pH, and in the whole cycle, sampling check does not regularly have assorted bacterium situation and growth indexes.

(3) 24 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium, micro capsule technology installs spissated bacterium additional VISOSE outer capsule, and this technology both makes it when storing and mixing with vehicle that protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-3.5 ℃ of conditions; Carry out 3 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 0.5%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 85%; Good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 0.5%, be finished product.

(5) every batch of product all passes through no Salmonellas detection.

The validity that microbiobacterial agent is applied to the biological prosthetic reinforcement schedule depends on the environment of place to use at that time.These envrionment conditionss have determined the efficient and the corresponding using dosage of degraded.The biodegradable time also depends on emulsification unit, envrionment conditions, blend level and other factors simultaneously.Through test shows, following physics and chemical parameters are grown up the most effective to bacterium:

1, pH value: 8.0-9.5.

2, temperature: 7.2 ℃-16.6 ℃.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount reach 5 mg/litre.The bacterium family of contained high adaptive faculty will accelerate 5 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

5, nitrogen: 10-15ppm.

6, phosphorus: 4-6ppm.

7, salinity: in seawater and fresh water, all be suitable for, can tolerate 40 ‰ salinity.

Other are identical with embodiment 1.

Embodiment 3

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 25%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 73%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 1.5%.

The preparation method of said new microbe agent, step is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 25% grows in substratum; After detecting; The inoculum of the first-generation is transferred in 250 liters of biological fermentation devices of aseptically process and carries out 16 hours growth, and said bacterial growth temperature is: 30.5 ℃.

(3) 20 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium.Micro capsule technology installs spissated bacterium additional VISOSE outer capsule.This technology both makes it when storing and mixing with vehicle, and protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-2.5 ℃ of conditions; Carry out 2 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 0.5%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 73%; Good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 1.5%, be finished product.

(5) every batch of product all passes through no Salmonellas detection.

1, pH value: 7.5-8.6.

2, temperature: 26.6 ℃-38.4 ℃.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount reach 6 mg/litre.The bacterium family of contained high adaptive faculty will accelerate 7 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

5, nitrogen: 15-20ppm.

6, phosphorus: 5-7ppm.

7, salinity: in seawater and fresh water, all be suitable for, can tolerate 30 ‰ salinity.

Other are identical with embodiment 1.

Embodiment 4

(1) said enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 4%;

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 23%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 70.5%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 2.5%.

The preparation method of said new microbe agent, step is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 23% grows in substratum; After detecting; The inoculum of the first-generation is transferred in 250 liters of biological fermentation devices of aseptically process and carries out 18 hours growth, and said bacterial growth temperature is: 47.2 ℃.

(3) 16 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium.Micro capsule technology installs spissated bacterium additional VISOSE outer capsule.This technology both makes it when storing and mixing with vehicle, and protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-1.5 ℃ of conditions; Carry out 4 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 4%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 70.5%; Good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 2.5%, be finished product.

(5) every batch of product all passes through no Salmonellas detection.

1, pH value: 6.6-7.2.

2, temperature: 39.8 ℃-55.3 ℃ come into force.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount 6 mg/litre.The bacterium family of contained high adaptive faculty will accelerate 6 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

5, nitrogen: 15-20ppm.

6, phosphorus: 8-10ppm.

7, salinity: in seawater and fresh water, all be suitable for, can tolerate 20 ‰.

Other are identical with embodiment 1.

Embodiment 5

(1) said enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 5%;

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 12%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 80%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 3%.

The preparation method of said new microbe agent, step is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 12% grows in substratum; After detecting; The inoculum of the first-generation is transferred in 250 liters of biological fermentation devices of aseptically process and carries out 20 hours growth, and said bacterial growth temperature is: 55.5 ℃.

(3) 21 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium.Micro capsule technology installs spissated bacterium additional VISOSE outer capsule.This technology both makes it when storing and mixing with vehicle, and protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-0.5 ℃ of condition; Carry out 3 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 5%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 80%; Good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 3%, be finished product.

(5) every batch of product all passes through no Salmonellas detection.

1, pH value: 7.5-8.4.

2, temperature: 50.8 ℃-65.5 ℃.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount reach 8 mg/litre.The bacterium family of contained high adaptive faculty will accelerate 6 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

5, nitrogen: 20-25ppm.

6, phosphorus: 10-15ppm.

7, salinity: in seawater and fresh water, all be suitable for, can tolerate 20 ‰ salinity.

Other are identical with embodiment 1.

Embodiment 6

(1) said enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 1%;

(2) said bacterium comprises that lactococcus lactis ssp (Lactococcus lactis) claims streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillus plantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomycescerevisiae), streptococcus faecalis (Streptococcus faecium) not only but also claim faecium (Enterococcus faecium), and it is formed weight percent and is: 2%;

(3) said nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 95%;

(4) said good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 2%.

Preparing method's step of said new microbe agent is following:

(1) bacterium through screening: lactococcus lactis ssp (Lactococcus lactis) is claimed streptococcus acidi lactici (Streptococcus lactis), plant lactobacillus (Lactobacillusplantarum), subtilis (Bacillus subtilis), cereuisiae fermentum or yeast saccharomyces cerevisiae (Saccharomyces cerevisiae), streptococcus faecalis (Streptococcusfaecium) not only but also is claimed faecium (Enterococcus faecium); It forms weight percent: 2% grows in substratum; After detecting; The inoculum of the first-generation is transferred to the growth of in 250 liters of biological fermentation devices of aseptically process, carrying out twenty four hours, and said bacterial growth temperature is: 28.5 ℃.

(2) after purity was confirmed in quality examination, inoculum was transferred in 5,000 liters of stainless steel fermentation containers of sealed, sterile.Aseptic sugar and oxygen supply are given bacterium and are controlled suitable pH.In the whole cycle, sampling check does not regularly have assorted bacterium situation and growth indexes.

(3) 10 hours, just can gather in the crops through the ultra centrifugation of gentleness and spissated bacterium.Micro capsule technology installs spissated bacterium additional VISOSE outer capsule.This technology both makes it when storing and mixing with vehicle, and protection against the tide also maintains vigour.

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, under-3 ℃ of conditions; Carry out 2 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, mixes other prescription: enzyme, nutrition and good antiscale property agent; Enzyme comprises proteolytic enzyme, glycase, cellulase, lypase, and its composition weight percent is: 1%; Nutrition comprises sucrose, whey, molasses, does not contain carriers such as wheat bran, rice bran, and its composition weight percent is: 95%; Good antiscale property agent sodium aluminum silicate (NaAlSi ₃O ₈) weight percent be: 2%, be packaging final prod behind the composition.

(5) every batch of product all passes through no Salmonellas detection.

1, pH value: 5.5-6.7.

2, temperature: 26 ℃-32 ℃.

3, dissolved oxygen: the aeration tank in WWT, dissolved oxygen amount 9 mg/litre.The bacterium family of contained high adaptive faculty will accelerate 6 times at metabolism and degradation speed to target substance in competent oxygen.In soil remediation, need suitable loose soil or ventilation oxygenating.

5, nitrogen: 10-20ppm.

6, phosphorus: 1-5ppm.

7, salinity: in seawater and fresh water, all be suitable for, can tolerate 10 ‰ salinity.

Other are identical with embodiment 1.

Claims

1. microbiobacterial agent, it is characterized in that: said microbiobacterial agent comprises enzyme and bacterium, nutrition and good antiscale property agent:

(3) said nutrition comprises sucrose, whey, molasses, does not contain wheat bran, rice bran carrier, and its composition weight percent is: 68～95%;

2. a kind of microbiobacterial agent according to claim 1 is characterized in that: said lactococcus lactis ssp is a streptococcus acidi lactici.

3. a kind of microbiobacterial agent according to claim 1 is characterized in that: said streptococcus faecalis is a faecium.

4. method for preparing the described microbiobacterial agent of claim 1, its step is following:

(4) subsequently product in the fluid refrigeration system by quick freezing, and at large-scale refrigeration chamber, freezing temp is at-4.5～0 ℃; Carry out 2～4 days lyophilize; Twice freezing dry process eliminated 95% moisture, guaranteed the high viability of bacterial classification, and mixing behind other system component is packaging final prod.